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Sequences

Sequence APAF1.eAug05

ATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACATCATGGATCACATGATTAGTGATGGAT
TTTTAACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACTTAAAAAAGATAATGATTC
CTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTCTCTTCTTCCAGTGGTAAA
GATTCAGTTAGTGGAATAACTTCGTATGTAAGGACAGTCCTGTGTGAAGGTGGAGTACCACAGAGGCCAGTTGTTTTTGTCACAAGGAAGAAGCTGGTGA
ATGCAATTCAGCAGAAGCTCTCCAAATTGAAAGGTGAACCAGGATGGGTCACCATACATGGAATGGCAGGCTGTGGGAAGTCTGTATTAGCTGCAGAAGC
TGTTAGAGATCATTCCCTTTTAGAAGGTTGTTTCCCAGGGGGAGTGCATTGGGTTTCAGTTGGGAAACAAGACAAATCTGGGCTTCTGATGAAACTGCAG
AATCTTTGCACACGGTTGGATCAGGATGAGAGTTTTTCCCAGAGGCTTCCACTTAATATTGAAGAGGCTAAAGACCGTCTCCGCATTCTGATGCTTCGCA
AACACCCAAGGTCTCTCTTGATCTTGGATGATGTTTGGGACTCTTGGGTGTTGAAAGCTTTTGACAGTCAGTGTCAGATTCTTCTTACAACCAGAGACAA
GAGTGTTACAGATTCAGTAATGGGTCCTAAATATGTAGTCCCTGTGGAGAGTTCCTTAGGAAAGGAAAAAGGACTTGAAATTTTATCCCTTTTTGTTAAT
ATGAAGAAGGCAGATTTGCCAGAACAAGCTCATAGTATTATAAAAGAATGTAAAGGCTCTCCCCTTGTAGTATCTTTAATTGGTGCACTTTTACGTGATT
TTCCCAATCGCTGGGAGTACTACCTCAAACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAATCTTCGTCTTATGATTATGAGGCTCTAGATGAAGC
CATGTCTATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGATTATTACACAGATCTTTCCATCCTTCAGAAGGACGTTAAGGTGCCTACAAAGGTGTTA
TGTATTCTCTGGGACATGGAAACTGAAGAAGTTGAAGACATACTGCAGGAGTTTGTAAATAAGTCTCTTTTATTCTGTGATCGGAATGGAAAGTCGTTTC
GTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATTGCAGCCAGCTTCAGGATCTACATAAGAAGATAATCACTCAGTTTCAGAGATA
TCACCAGCCGCATACTCTTTCACCAGATCAGGAAGACTGTATGTATTGGTACAACTTTCTGGCCTATCACATGGCCAGTGCCAAGATGCACAAGGAACTT
TGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAACAGAACTTGTAGGCCCTGCTCATCTGATTCATGAATTTGTGGAATACAGACATATACTAG
ATGAAAAGGATTGTGCAGTCAGTGAGAATTTTCAGGAGTTTTTATCTTTAAATGGACACCTTCTTGGACGACAGCCATTTCCTAATATTGTACAACTGGG
TCTCTGTGAGCCGGAAACTTCAGAAGTTTATCAGCAAGCTAAGCTGCAGGCCAAGCAGGAGGTCGATAATGGAATGCTTTACCTGGAATGGATAAACAAA
AAAAACATCACGAATCTTTCCCGCTTAGTTGTCCGCCCCCACACAGATGCTGTTTACCATGCCTGCTTTTCTGAGGATGGTCAGAGAATAGCTTCTTGTG
GAGCTGATAAAACCTTACAGGTGTTCAAAGCTGAAACAGGAGAGAAACTTCTAGAAATCAAGGCTCATGAGGATGAAGTGCTTTGTTGTGCATTCTCTAC
AGATGACAGATTTATAGCAACCTGCTCAGTGGATAAAAAAGTGAAGATTTGGAATTCTATGACTGGGGAACTAGTACACACCTATGATGAGCACTCAGAG
CAAGTCAATTGCTGCCATTTCACCAACAGTAGTCATCATCTTCTCTTAGCCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGGGATTTGAATCAAAAAG
AATGTCGAAATACCATGTTTGGTCATACAAATTCAGTCAATCACTGCAGATTTTCACCAGATGATAAGCTTTTGGCTAGTTGTTCAGCTGATGGAACCTT
AAAGCTTTGGGATGCGACATCAGCAAATGAGAGGAAAAGCATTAATGTGAAACAGTTCTTCCTAAATTTGGAGGACCCTCAAGAGGATATGGAAGTGATA
GTGAAGTGTTGTTCGTGGTCTGCTGATGGTGCAAGGATAATGGTGGCAGCAAAAAATAAAATCTTTTTGTGGAATACAGACTCACGTTCAAAGGTGGCTG
ATTGCAGAGGACATTTAAGTTGGGTTCATGGTGTGATGTTTTCTCCTGATGGATCATCATTTTTGACATCTTCTGATGACCAGACAATCAGGCTCTGGGA
GACAAAGAAAGTATGTAAGAACTCTGCTGTAATGTTAAAGCAAGAAGTAGATGTTGTGTTTCAAGAAAATGAAGTGATGGTCCTTGCAGTTGACCATATA
AGACGTCTGCAACTCATTAATGGAAGAACAGGTCAGATTGATTATCTGACTGAAGCTCAAGTTAGCTGCTGTTGCTTAAGTCCACATCTTCAGTACATTG
CATTTGGAGATGAAAATGGAGCCATTGAGATTTTAGAACTTGTAAACAATAGAATCTTCCAGTCCAGGTTTCAGCACAAGAAAACTGTATGGCACATCCA
GTTCACAGCCGATGAGAAGACTCTTATTTCAAGTTCTGATGATGCTGAAATTCAGGTATGGAATTGGCAATTGGACAAATGTATCTTTCTACGAGGCCAT
CAGGAAACAGTGAAAGACTTTAGACTCTTGAAAAATTCAAGACTGCTTTCTTGGTCATTTGATGGAACAGTGAAGGTATGGAATATTATTACTGGAAATA
AAGAAAAAGACTTTGTCTGTCACCAGGGTACAGTACTTTCTTGTGACATTTCTCACGATGCTACCAAGTTTTCATCTACCTCTGCTGACAAGACTGCAAA
GATATGGAATGTCTCAAACGGTGAGCTTCTTCATTTGTGTGCTCCGCTTTCAGAAGAAGGAGCTGCTACCCATGGAGGCTGGGTGACTGACCTTTGCTTT
TCTCCAGATGGCAAAATGCTTATCTCTGCTGGAGGATATATTAAGTGGTGGAACGTTGTCACTGGGGAATCCTCACAGACCTTCTACACAAATGGAACCA
ATCTTAAGAAAATACACGTGTCCCCTGACTTCAAAACATATGTGACTGTGGATAATCTTGGTATTTTATATATTTTACAGACTTTAGAATAAAATAGTTA
AGCATTAATGTAGTTGAACTTTTTAAATTTTTGAATTGGAAAAAAATTCTAATGAAACCCTGATATCAACTTTTTATAAAGCTCTTAATTGTTGTGCAGT
ATTGCATTCATTACAAAAGTGTTTGTGGTTGGATGAATAATATTAATGTAGCTTTTTCCCAAATGAACATACCTTTAATCTTGTTTTTCATGATCATCAT
TAACAGTTTGTCCTTAGGATGCAAATGAAAATGTGAATACATACCTTGTTGTACTGTTGGTAAAATTCTGTCTTGATGCATTCAAAATGGTTGACATAAT
TAATGAGAAGAATTTGGAAGAAATTGGTATTTTAATACTGTCTGTATTTATTACTGTTATGCAGGCTGTGCCTCAGGGTAGCAGTGGCCTGCTTTTTGAA
CCACACTTACCCCAAGGGGGTTTTGTTCTCCTAAATACAATCTTAGAGGTTTTTTGCACTCTTTAAATTTGCTTTAAAAATATTGTGTCTGTGTGCATAG
TCTGCAGCATTTCCTTTAATTGACTCAATAAGTGAGTCTTGGATTTAGCAGGCCCCCCCACCTTTTTTTTTTGTTTTTGGAGACAGAGTCTTGCTTTGTT
GCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACCACAATCGCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTA
CAGGTGTGCGCACATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCGGGATGGTCTCGATCTCTTGACCTCATGATCTA
CCCGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACCGTGCCTGGCCAGGCCCCTTCTCTTTTAATGGAGACAGGGTCTTGCACTATCACC
CAGGCTGGAGTGCAGTGGCATAATCATACCTCATTGCAGCCTCAGACTCCTGGGTTCAAGCAATCCTCCTGCCTCAGCCTCCCAAGTAGCTGAGACTACA
GGCACGAGCCACCACACCCAGCTAATTTTTAAGTTTTCTTGTAGAGACAGGGTCTCACTATGTTGTCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTAAT
CCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTGCAGATATGAGCCACTGGCCTGGCCTTCAGCAGTTCTTTTTGTGAAGTAAAACTTGTATGTTGGAAA
GAGTAGATTTTATTGGTCTACCCTTTTCTCACTGTAGCTGCTGGCAGCCCTGTGCCATATCTGGACTCTAGTTGTCAGTATCTGAGTTGGACACTATTCC
TGCTCCCTCTTGTTTCTTACATATCAGACTTCTTACTTGAATGAAACCTGATCTTTCCTAATCCTCACTTTTTTCTTTTTTAAAAAGCAGTTTCTCCACT
GCTAAATGTTAGTCATTGAGGTGGGGCCAATTTTAATCATAAGCCTTAATAAGATTTTTCTAAGAAATGTGAAATAGAACAATTTTCATCTAATTCCATT
TACTTTTAGATGAATGGCATTGTGAATGCCATTCTTTTAATGAATTTCAAGAGAATTCTCTGGTTTTCTGTGTAATTCCAGATGAGTCACTGTAACTCTA
GAAGATTAACCTTCCAGCCAACCTATTTTCCTTTCCCTTGTCTCTCTCATCCTCTTTTCCTTCCTTCTTTCCTTTCTCTTCTTTTATCTCCAAGGTTAAT
CAGGAAAAATAGCTTTTGACAGGGGAAAAAACTCAATAACTAGCTATTTTTGACCTCCTGATCAGGAACTTTAGTTGAAGCGTAAATCTAAAGAAACATT
TTCTCTGAAATATATTATTAAGGGCAATGGAGATAAATTAATAGTAGATGTGGTTCCCAGAAAATATAATCAAAATTCAAAGATTTTTTTTGTTTCTGTA
ACTGGAACTAAATCAAATGATTACTAGTGTTAATAGTAGATAACTTGTTTTTATTGTTGGTGCATATTAGTATAACTGTGGGGTAGGTCGGGGAGAGGGT
AAGGGAATAGATCACTCAGATGTATTTTAGATAAGCTATTTAGCCTTTGATGGAATCATAAATACAGTGAATACAATCCTTTGCATTGTTAAGGAGGTTT
TTTGTTTTTAAATGGTGGGTCAAGGAGCTAGTTTACAGGCTTACTGTGATTTAAGCAAATGTGAAAAGTGAAACCTTAATTTTATCAAAAGAAATTTCTG
TAAATGGTATGTCTCCTTAGAATACCCAAATCATAATTTTATTTGTACACACTGTTAGGGGCTCATCTCATGTAGGCAGAGTATAAAGTATTACCTTTTG
GAATTAAAAGCCACTGACTGTTATAAAGTATAACAACACACATCAGGTTTTAAAAAGCCTTGAATGGCCCTTGTCTTAAAAAGAAATTAGGAGCCAGGTG
CGGTGGCACGTGCCTGTAATCCCAGCTCCTTGGGAGGCTAAGACAGGAGGATTCCTTGAGCCCTGGAGTTTGAGTCCAGCCTGGGTGACATAGCAAGACC
CTGTCTTAAAAGAAAAATGGGAAGAAAGACAAGGTAACATGAAGAAAGAAGAGATACCTAGTATGATGGAGCTGCAAATTTCATGGCAGTTCATGCAGTC
GGTCAAGAGGAGGATTTTGTTTTGTAGTTTGCAGATGAGCATTTCTAAAGCATTTTCCCTTGCTGTATTTTTTTGTATTATAAATTACATTGGACTTCAT
ATATATAATTTTTTTTTACATTATATGTCTCTTGTATGTTTTGAAACTCTTGTATTTATGATATAGCTTATATGATTTTTTTGCCTTGGTATACATTTTA
AAATATGAATTTAAAAAATTTTTGTAAAAATAAAATTCACAAAATTGTTTTGAAAAAC
  • APAF1-3 (forward primer)
  • APAF1-4 (reverse primer)
  • Single exon skipping

Frame 0

MDAKARNCLLQHREALEKDIKTSYIMDHMISDG
FLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDS
YVSFYNALLHEGYKDLAALLHDGIPVVSSSSGK
DSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLV
NAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEA
VRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQ
NLCTRLDQDESFSQRLPLNIEEAKDRLRILMLR
KHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDK
SVTDSVMGPKYVVPVESSLGKEKGLEILSLFVN
MKKADLPEQAHSIIKECKGSPLVVSLIGALLRD
FPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEA
MSISVEMLREDIKDYYTDLSILQKDVKVPTKVL
CILWDMETEEVEDILQEFVNKSLLFCDRNGKSF
RYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRY
HQPHTLSPDQEDCMYWYNFLAYHMASAKMHKEL
CALMFSLDWIKAKTELVGPAHLIHEFVEYRHIL
DEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLG
LCEPETSEVYQQAKLQAKQEVDNGMLYLEWINK
KNITNLSRLVVRPHTDAVYHACFSEDGQRIASC
GADKTLQVFKAETGEKLLEIKAHEDEVLCCAFST
DDRFIATCSVDKKVKIWNSMTGELVHTYDEHSE
QVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQK
ECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTL
KLWDATSANERKSINVKQFFLNLEDPQEDMEVI
VKCCSWSADGARIMVAAKNKIFLWNTDSRSKVA
DCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWE
TKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHI
RRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYI
AFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQ
FTADEKTLISSSDDAEIQVWNWQLDKCIFLRGH
QETVKDFRLLKNSRLLSWSFDGTVKVWNIITGN
KEKDFVCHQGTVLSCDISHDATKFSSTSADKTAK
IWNVSNGELLHLCAPLSEEGAATHGGWVTDLCF
SPDGKMLISAGGYIKWWNVVTGESSQTFYTNGT
NLKKIHVSPDFKTYVTVDNLGILYILQTLE*NS*
ALM*LNFLNF*IGKKF**NPDINFL*SS*LLCS
IAFITKVFVVG*IILM*LFPK*TYL*SCFS*SS
LTVCP*DANENVNTYLVVLLVKFCLDAFKMVDII
NEKNLEEIGILILSVFITVMQAVPQGSSGLLFE
PHLPQGGFVLLNTILEVFCTL*ICFKNIVSVCI
VCSISFN*LNK*VLDLAGPPTFFFCFWRQSLALL
PGWSAVARSRLTTIAASWVQAILLPQPPE*LGL
QVCAHARLIFVFLVETGFHHVGRDGLDLLTS*S
TRLGLPKC*DYRREPPCLARPLLF*WRQGLALSP
RLECSGIIIPHCSLRLLGSSNPPASASQVAETT
GTSHHTQLIFKFSCRDRVSLCCLGWS*TLGLK*
SSCLSLPKCWDCRYEPLAWPSAVLFVK*NLYVGK
SRFYWSTLFSL*LLAALCHIWTLVVSI*VGHYS
CSLLFLTYQTSYLNET*SFLILTFFFFKKQFLH
C*MLVIEVGPILIISLNKIFLRNVK*NNFHLIPF
TFR*MAL*MPFF**ISREFSGFLCNSR*VTVTL
ED*PSSQPIFLSLVSLILFSFLLSFLFFYLQG*
SGKIAFDRGKNSITSYF*PPDQEL*LKRKSKETF
SLKYIIKGNGDKLIVDVVPRKYNQNSKIFFVSV
TGTKSNDY*C***ITCFYCWCILV*LWGRSGRG
*GNRSLRCILDKLFSL*WNHKYSEYNPLHC*GGF
LFLNGGSRS*FTGLL*FKQM*KVKP*FYQKKFL
*MVCLLRIPKS*FYLYTLLGAHLM*AEYKVLPF
GIKSH*LL*SITTHIRF*KALNGPCLKKKLGARC
GGTCL*SQLLGRLRQEDSLSPGV*VQPG*HSKT
LS*KKNGKKDKVT*RKKRYLV*WSCKFHGSSCS
RSRGGFCFVVCR*AFLKHFPLLYFFVL*ITLDFI
YIIFFYIICLLYVLKLLYL*YSLYDFFALVYIL
KYEFKKFL*K*NSQNCFEK

Frame 1

WMQKLEIVCFNIEKLWKRTSRHPTSWIT*LVMD
F*QYQKRKK*EMSPLNSKEQLC*LK*YLKKIMI
PTYHSTMLYYMKDIKILLPFSMMAFLLSLLPVVK
IQLVE*LRM*GQSCVKVEYHRGQLFLSQGRSW*
MQFSRSSPN*KVNQDGSPYMEWQAVGSLY*LQK
LLEIIPF*KVVSQGECIGFQLGNKTNLGF**NCR
IFAHGWIRMRVFPRGFHLILKRLKTVSAF*CFA
NTQGLS*SWMMFGTLGC*KLLTVSVRFFLQPET
RVLQIQ*WVLNM*SLWRVP*ERKKDLKFYPFLLI
*RRQICQNKLIVL*KNVKALPL*YL*LVHFYVI
FPIAGSTTSNSFRISSLRE*GNLRLMIMRL*MK
PCL*VLKCSEKTSKIITQIFPSFRRTLRCLQRCY
VFSGTWKLKKLKTYCRSL*ISLFYSVIGMESRF
VIIYMIFK*IFLQRRIAASFRIYIRR*SLSFRD
ITSRILFHQIRKTVCIGTTFWPITWPVPRCTRNF
VL*CFPWIGLKQKQNL*ALLI*FMNLWNTDIY*
MKRIVQSVRIFRSFYL*MDTFLDDSHFLILYNW
VSVSRKLQKFISKLSCRPSRRSIMECFTWNG*TK
KTSRIFPA*LSAPTQMLFTMPAFLRMVRE*LLV
ELIKPYRCSKLKQERNF*KSRLMRMKCFVVHSL
QMTDL*QPAQWIKK*RFGIL*LGN*YTPMMSTQS
KSIAAISPTVVIIFS*PLGQVTASSNFGI*IKK
NVEIPCLVIQIQSITADFHQMISFWLVVQLMEP
*SFGMRHQQMRGKALM*NSSS*IWRTLKRIWK**
*SVVRGLLMVQG*WWQQKIKSFCGIQTHVQRWL
IAEDI*VGFMV*CFLLMDHHF*HLLMTRQSGSG
RQRKYVRTLL*C*SKK*MLCFKKMK*WSLQLTI*
DVCNSLMEEQVRLII*LKLKLAAVA*VHIFSTL
HLEMKMEPLRF*NL*TIESSSPGFSTRKLYGTS
SSQPMRRLLFQVLMMLKFRYGIGNWTNVSFYEAI
RKQ*KTLDS*KIQDCFLGHLMEQ*RYGILLLEI
KKKTLSVTRVQYFLVTFLTMLPSFHLPLLTRLQ
RYGMSQTVSFFICVLRFQKKELLPMEAG*LTFAF
LQMAKCLSLLEDILSGGTLSLGNPHRPSTQMEP
ILRKYTCPLTSKHM*LWIILVFYIFYRL*NKIV
KH*CS*TF*IFELEKNSNETLISTFYKALNCCAV
LHSLQKCLWLDE*Y*CSFFPNEHTFNLVFHDHH
*QFVLRMQMKM*IHTLLYCW*NSVLMHSKWLT*
LMRRIWKKLVF*YCLYLLLLCRLCLRVAVACFLN
HTYPKGVLFS*IQS*RFFALFKFALKILCLCA*
SAAFPLIDSISESWI*QAPPPFFFVFGDRVLLC
CQAGVQWRDLGSPQSLPPGFKQFSCLSLPSSWDY
RCAHMPG*FLYF**RRGFTMLAGMVSIS*PHDL
PALASQSAEITGVSHRAWPGPFSFNGDRVLHYH
PGWSAVA*SYLIAASDSWVQAILLPQPPK*LRLQ
ARATTPS*FLSFLVETGSHYVV*AGLELLASSN
PPASASQSVGIADMSHWPGLQQFFL*SKTCMLE
RVDFIGLPFSHCSCWQPCAISGL*LSVSELDTIP
APSCFLHIRLLT*MKPDLS*SSLFSFLKSSFST
AKC*SLRWGQF*S*ALIRFF*EM*NRTIFI*FH
LLLDEWHCECHSFNEFQENSLVFCVIPDESL*L*
KINLPANLFSFPLSLSSSFPSFFPFSSFISKVN
QEK*LLTGEKTQ*LAIFDLLIRNFS*SVNLKKH
FL*NILLRAMEIN***MWFPENIIKIQRFFLFL*
LELNQMITSVNSR*LVFIVGAY*YNCGVGRGEG
KGIDHSDVF*ISYLAFDGIINTVNTILCIVKEV
FCF*MVGQGASLQAYCDLSKCEK*NLNFIKRNFC
KWYVSLEYPNHNFICTHC*GLISCRQSIKYYLL
ELKATDCYKV*QHTSGFKKP*MALVLKRN*EPG
AVARACNPSSLGG*DRRIP*ALEFESSLGDIARP
CLKRKMGRKTR*HEERRDT*YDGAANFMAVHAV
GQEEDFVL*FADEHF*SIFPCCIFLYYKLHWTS
YI*FFFTLYVSCMF*NSCIYDIAYMIFLPWYTF*
NMNLKNFCKNKIHKIVLKN

Frame 2

GCKSSKLFAST*RSSGKGHQDILHHGSHD**WI
FNNIRRGKSKK*AHSTAKSSYAD*NDT*KR**F
LRIILQCSTT*RI*RSCCPSP*WHSCCLFFQW*
RFS*WNNFVCKDSPV*RWSTTEASCFCHKEEAGE
CNSAEALQIER*TRMGHHTWNGRLWEVCISCRS
C*RSFPFRRLFPRGSALGFSWETRQIWASDETA
ESLHTVGSG*EFFPEAST*Y*RG*RPSPHSDASQ
TPKVSLDLG*CLGLLGVESF*QSVSDSSYNQRQ
ECYRFSNGS*ICSPCGEFLRKGKRT*NFIPFC*
YEEGRFARTSS*YYKRM*RLSPCSIFNWCTFT*F
SQSLGVLPQTASE*AV*ENKEIFVL*L*GSR*S
HVYKC*NAQRRHQRLLHRSFHPSEGR*GAYKGV
MYSLGHGN*RS*RHTAGVCK*VSFIL*SEWKVVS
LLFT*SSSRFSYREELQPASGST*EDNHSVSEI
SPAAYSFTRSGRLYVLVQLSGLSHGQCQDAQGT
LCFNVFPGLD*SKNRTCRPCSSDS*ICGIQTYTR
*KGLCSQ*EFSGVFIFKWTPSWTTAIS*YCTTG
SL*AGNFRSLSAS*AAGQAGGR*WNALPGMDKQ
KKHHESFPLSCPPPHRCCLPCLLF*GWSENSFLW
S**NLTGVQS*NRRETSRNQGS*G*SALLCILY
R*QIYSNLLSG*KSEDLEFYDWGTSTHL**ALR
ASQLLPFHQQ*SSSSLSHWVK*LLPQTLGFESKR
MSKYHVWSYKFSQSLQIFTR**AFG*LFS*WNL
KALGCDISK*EEKH*CETVLPKFGGPSRGYGSD
SEVLFVVC*WCKDNGGSKK*NLFVEYRLTFKGG*
LQRTFKLGSWCDVFS*WIIIFDIF**PDNQALG
DKESM*ELCCNVKARSRCCVSRK*SDGPCS*PY
KTSATH*WKNRSD*LSD*SSS*LLLLKSTSSVHC
IWR*KWSH*DFRTCKQ*NLPVQVSAQENCMAHP
VHSR*EDSYFKF**C*NSGMELAIGQMYLSTRP
SGNSERL*TLEKFKTAFLVI*WNSEGMEYYYWK*
RKRLCLSPGYSTFL*HFSRCYQVFIYLC*QDCK
DMECLKR*ASSFVCSAFRRRSCYPWRLGD*PLL
FSRWQNAYLCWRIY*VVERCHWGILTDLLHKWNQ
S*ENTRVP*LQNICDCG*SWYFIYFTDFRIK*L
SINVVELFKFLNWKKILMKP*YQLFIKLLIVVQ
YCIHYKSVCGWMNNINVAFSQMNIPLILFFMIII
NSLSLGCK*KCEYIPCCTVGKILS*CIQNG*HN
**EEFGRNWYFNTVCIYYCYAGCASG*QWPAF*
TTLTPRGFCSPKYNLRGFLHSLNLL*KYCVCVHS
LQHFL*LTQ*VSLGFSRPPHLFFLFLETESCFV
ARLECSGAISAHHNRCLLGSSNSPASASRVAGT
TGVRTCQANFCIFSRDGVSPCWPGWSRSLDLMIY
PPWPPKVLRLQA*ATVPGQAPSLLMETGSCTIT
QAGVQWHNHTSLQPQTPGFKQSSCLSLPSS*DY
RHEPPHPANF*VFL*RQGLTMLSRLVLNSWPQVI
LLPQPPKVLGLQI*ATGLAFSSSFCEVKLVCWK
E*ILLVYPFLTVAAGSPVPYLDSSCQYLSWTLF
LLPLVSYISDFLLE*NLIFPNPHFFLF*KAVSPL
LNVSH*GGANFNHKP**DFSKKCEIEQFSSNSI
YF*MNGIVNAILLMNFKRILWFSV*FQMSHCNS
RRLTFQPTYFPFPCLSHPLFLPSFLSLLLSPRLI
RKNSF*QGKKLNN*LFLTS*SGTLVEA*I*RNI
FSEIYY*GQWR*INSRCGSQKI*SKFKDFFCFC
NWN*IK*LLVLIVDNLFLLLVHISITVG*VGERV
RE*ITQMYFR*AI*PLMES*IQ*IQSFALLRRF
FVFKWWVKELVYRLTVI*ANVKSETLILSKEIS
VNGMSP*NTQIIILFVHTVRGSSHVGRV*SITFW
N*KPLTVIKYNNTHQVLKSLEWPLS*KEIRSQV
RWHVPVIPAPWEAKTGGFLEPWSLSPAWVT*QD
PVLKEKWEERQGNMKKEEIPSMMELQISWQFMQS
VKRRILFCSLQMSISKAFSLAVFFCIINYIGLH
IYNFFLHYMSLVCFETLVFMI*LI*FFCLGIHF
KI*I*KIFVKIKFTKLF*K

Sequence APAF1.dAug05

ATGAGCCGTGGCAGGAGTGCGCGGCGGCAGCGGTGGCCGCCCCTTGGGGCTTGGGGTGTGTTTATTTGCATAAGCGGGCGCGCGCCGTCCGGGCTGGGTG
GATCCGGCGGGATTTGACTGCTCCGCTGTCCAGAGGCGGAGAAGAAGAGGTAGCGAGTGGACGTGACTGCTCTATCCCGGGCAAAAGGGATAGAACCAGA
GGTGGGGAGTCTGGGCAGTCGGCGACCCGCGAAGACTTGAGGTGCCGCAGCGGCATCCGGAGTAGCGCCGGGCTCCCTCCGGGGTGCAGCCGCCGTCGGG
GGAAGGGCGCCACAGGCCGGGAAGACCTCCTCCCTTTGTGTCCAGTAGTGGGGTCCACCGGAGGGCGGCCCGTGGGCCGGGCCTCACCGCGGCGCTCCGG
GACTGTGGGGTCAGGCTGCGTTGGGTGGACGCCCACCTCGCCAACCTTCGGAGGTCCCTGGGGGTCTTCGTGCGCCCCGGGGCTGCAGAGATCCAGGGGA
GGCGCCTGTGAGGCCCGGACCTGCCCCGGGGCGAAGGGTATGTGGCGAGACAGAGCCCTGCACCCCTAATTCCCGGTGGAAAACTCCTGTTGCCGTTTCC
CTCCACCGGCCTGGAGTCTCCCAGTCTTGTCCCGGCAGTGCCGCCCTCCCCACTAAGACCTAGGCGCAAAGGCTTGGCTCATGGTTGACAGCTCAGAGAG
AGAAAGATCTGAGGGAAGATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACATCATGGATC
ACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACT
TAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TCTTCTTCCAGTGTAAGGACAGTCCTGTGTGAAGGTGGAGTACCACAGAGGCCAGTTGTTTTTGTCACAAGGAAGAAGCTGGTGAATGCAATTCAGCAGA
AGCTCTCCAAATTGAAAGGTGAACCAGGATGGGTCACCATACATGGAATGGCAGGCTGTGGGAAGTCTGTATTAGCTGCAGAAGCTGTTAGAGATCATTC
CCTTTTAGAAGGTTGTTTCCCAGGGGGAGTGCATTGGGTTTCAGTTGGGAAACAAGACAAATCTGGGCTTCTGATGAAACTGCAGAATCTTTGCACACGG
TTGGATCAGGATGAGAGTTTTTCCCAGAGGCTTCCACTTAATATTGAAGAGGCTAAAGACCGTCTCCGCATTCTGATGCTTCGCAAACACCCAAGGTCTC
TCTTGATCTTGGATGATGTTTGGGACTCTTGGGTGTTGAAAGCTTTTGACAGTCAGTGTCAGATTCTTCTTACAACCAGAGACAAGAGTGTTACAGATTC
AGTAATGGGTCCTAAATATGTAGTCCCTGTGGAGAGTTCCTTAGGAAAGGAAAAAGGACTTGAAATTTTATCCCTTTTTGTTAATATGAAGAAGGCAGAT
TTGCCAGAACAAGCTCATAGTATTATAAAAGAATGTAAAGGCTCTCCCCTTGTAGTATCTTTAATTGGTGCACTTTTACGTGATTTTCCCAATCGCTGGG
AGTACTACCTCAAACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAATCTTCGTCTTATGATTATGAGGCTCTAGATGAAGCCATGTCTATAAGTGT
TGAAATGCTCAGAGAAGACATCAAAGATTATTACACAGATCTTTCCATCCTTCAGAAGGACGTTAAGGTGCCTACAAAGGTGTTATGTATTCTCTGGGAC
ATGGAAACTGAAGAAGTTGAAGACATACTGCAGGAGTTTGTAAATAAGTCTCTTTTATTCTGTGATCGGAATGGAAAGTCGTTTCGTTATTATTTACATG
ATCTTCAAGTAGATTTTCTTACAGAGAAGAATTGCAGCCAGCTTCAGGATCTACATAAGAAGATAATCACTCAGTTTCAGAGATATCACCAGCCGCATAC
TCTTTCACCAGATCAGGAAGACTGTATGTATTGGTACAACTTTCTGGCCTATCACATGGCCAGTGCCAAGATGCACAAGGAACTTTGTGCTTTAATGTTT
TCCCTGGATTGGATTAAAGCAAAAACAGAACTTGTAGGCCCTGCTCATCTGATTCATGAATTTGTGGAATACAGACATATACTAGATGAAAAGGATTGTG
CAGTCAGTGAGAATTTTCAGGAGTTTTTATCTTTAAATGGACACCTTCTTGGACGACAGCCATTTCCTAATATTGTACAACTGGGTCTCTGTGAGCCGGA
AACTTCAGAAGTTTATCAGCAAGCTAAGCTGCAGGCCAAGCAGGAGGTCGATAATGGAATGCTTTACCTGGAATGGATAAACAAAAAAAACATCACGAAT
CTTTCCCGCTTAGTTGTCCGCCCCCACACAGATGCTGTTTACCATGCCTGCTTTTCTGAGGATGGTCAGAGAATAGCTTCTTGTGGAGCTGATAAAACCT
TACAGGTGTTCAAAGCTGAAACAGGAGAGAAACTTCTAGAAATCAAGGCTCATGAGGATGAAGTGCTTTGTTGTGCATTCTCTACAGATGACAGATTTAT
AGCAACCTGCTCAGTGGATAAAAAAGTGAAGATTTGGAATTCTATGACTGGGGAACTAGTACACACCTATGATGAGCACTCAGAGCAAGTCAATTGCTGC
CATTTCACCAACAGTAGTCATCATCTTCTCTTAGCCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGGGATTTGAATCAAAAAGAATGTCGAAATACCA
TGTTTGGTCATACAAATTCAGTCAATCACTGCAGATTTTCACCAGATGATAAGCTTTTGGCTAGTTGTTCAGCTGATGGAACCTTAAAGCTTTGGGATGC
GACATCAGCAAATGAGAGGAAAAGCATTAATGTGAAACAGTTCTTCCTAAATTTGGAGGACCCTCAAGAGGATATGGAAGTGATAGTGAAGTGTTGTTCG
TGGTCTGCTGATGGTGCAAGGATAATGGTGGCAGCAAAAAATAAAATCTTTTTGTGGAATACAGACTCACGTTCAAAGGTGGCTGATTGCAGAGGACATT
TAAGTTGGGTTCATGGTGTGATGTTTTCTCCTGATGGATCATCATTTTTGACATCTTCTGATGACCAGACAATCAGGCTCTGGGAGACAAAGAAAGTATG
TAAGAACTCTGCTGTAATGTTAAAGCAAGAAGTAGATGTTGTGTTTCAAGAAAATGAAGTGATGGTCCTTGCAGTTGACCATATAAGACGTCTGCAACTC
ATTAATGGAAGAACAGGTCAGATTGATTATCTGACTGAAGCTCAAGTTAGCTGCTGTTGCTTAAGTCCACATCTTCAGTACATTGCATTTGGAGATGAAA
ATGGAGCCATTGAGATTTTAGAACTTGTAAACAATAGAATCTTCCAGTCCAGGTTTCAGCACAAGAAAACTGTATGGCACATCCAGTTCACAGCCGATGA
GAAGACTCTTATTTCAAGTTCTGATGATGCTGAAATTCAGGTATGGAATTGGCAATTGGACAAATGTATCTTTCTACGAGGCCATCAGGAAACAGTGAAA
GACTTTAGACTCTTGAAAAATTCAAGACTGCTTTCTTGGTCATTTGATGGAACAGTGAAGGTATGGAATATTATTACTGGAAATAAAGAAAAAGACTTTG
TCTGTCACCAGGGTACAGTACTTTCTTGTGACATTTCTCACGATGCTACCAAGTTTTCATCTACCTCTGCTGACAAGACTGCAAAGATCTGGAGTTTTGA
TCTCCTTTTGCCACTTCATGAATTGAGGGGCCACAACGGCTGTGTGCGCTGCTCTGCCTTCTCTGTGGACAGTACCCTGCTGGCAACGGGAGATGACAAT
GGAGAAATCAGGATATGGAATGTCTCAAACGGTGAGCTTCTTCATTTGTGTGCTCCGCTTTCAGAAGAAGGAGCTGCTACCCATGGAGGCTGGGTGACTG
ACCTTTGCTTTTCTCCAGATGGCAAAATGCTTATCTCTGCTGGAGGATATATTAAGTGGTGGAACGTTGTCACTGGGGAATCCTCACAGACCTTCTACAC
AAATGGAACCAATCTTAAGAAAATACACGTGTCCCCTGACTTCAAAACATATGTGACTGTGGATAATCTTGGTATTTTATATATTTTACAGACTTTAGAA
TAAAATAGTTAAGCATTAATGTAGTTGAACTTTTTAAATTTTTGAATTGGAAAAAAATTCTAATGAAACCCTGATATCAACTTTTTATAAAGCTCTTAAT
TGTTGTGCAGTATTGCATTCATTACAAAAGTGTTTGTGGTTGGATGAATAATATTAATGTAGCTTTTTCCCAAATGAACATACCTTTAATCTTGTTTTTC
ATGATCATCATTAACAGTTTGTCCTTAGGATGCAAATGAAAATGTGAATACATACCTTGTTGTACTGTTGGTAAAATTCTGTCTTGATGCATTCAAAATG
GTTGACATAATTAATGAGAAGAATTTGGAAGAAATTGGTATTTTAATACTGTCTGTATTTATTACTGTTATGCAGGCTGTGCCTCAGGGTAGCAGTGGCC
TGCTTTTTGAACCACACTTACCCCAAGGGGGTTTTGTTCTCCTAAATACAATCTTAGAGGTTTTTTGCACTCTTTAAATTTGCTTTAAAAATATTGTGTC
TGTGTGCATAGTCTGCAGCATTTCCTTTAATTGACTCAATAAGTGAGTCTTGGATTTAGCAGGCCCCCCCACCTTTTTTTTTTGTTTTTGGAGACAGAGT
CTTGCTTTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACCACAATCGCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGT
AGCTGGGACTACAGGTGTGCGCACATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCGGGATGGTCTCGATCTCTTGAC
CTCATGATCTACCCGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACCGTGCCTGGCCAGGCCCCTTCTCTTTTAATGGAGACAGGGTCTT
GCACTATCACCCAGGCTGGAGTGCAGTGGCATAATCATACCTCATTGCAGCCTCAGACTCCTGGGTTCAAGCAATCCTCCTGCCTCAGCCTCCCAAGTAG
CTGAGACTACAGGCACGAGCCACCACACCCAGCTAATTTTTAAGTTTTCTTGTAGAGACAGGGTCTCACTATGTTGTCTAGGCTGGTCTTGAACTCTTGG
CCTCAAGTAATCCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTGCAGATATGAGCCACTGGCCTGGCCTTCAGCAGTTCTTTTTGTGAAGTAAAACTTG
TATGTTGGAAAGAGTAGATTTTATTGGTCTACCCTTTTCTCACTGTAGCTGCTGGCAGCCCTGTGCCATATCTGGACTCTAGTTGTCAGTATCTGAGTTG
GACACTATTCCTGCTCCCTCTTGTTTCTTACATATCAGACTTCTTACTTGAATGAAACCTGATCTTTCCTAATCCTCACTTTTTTCTTTTTTAAAAAGCA
GTTTCTCCACTGCTAAATGTTAGTCATTGAGGTGGGGCCAATTTTAATCATAAGCCTTAATAAGATTTTTCTAAGAAATGTGAAATAGAACAATTTTCAT
CTAATTCCATTTACTTTTAGATGAATGGCATTGTGAATGCCATTCTTTTAATGAATTTCAAGAGAATTCTCTGGTTTTCTGTGTAATTCCAGATGAGTCA
CTGTAACTCTAGAAGATTAACCTTCCAGCCAACCTATTTTCCTTTCCCTTGTCTCTCTCATCCTCTTTTCCTTCCTTCTTTCCTTTCTCTTCTTTTATCT
CCAAGGTTAATCAGGAAAAATAGCTTTTGACAGGGGAAAAAACTCAATAACTAGCTATTTTTGACCTCCTGATCAGGAACTTTAGTTGAAGCGTAAATCT
AAAGAAACATTTTCTCTGAAATATATTATTAAGGGCAATGGAGATAAATTAATAGTAGATGTGGTTCCCAGAAAATATAATCAAAATTCAAAGATTTTTT
TTGTTTCTGTAACTGGAACTAAATCAAATGATTACTAGTGTTAATAGTAGATAACTTGTTTTTATTGTTGGTGCATATTAGTATAACTGTGGGGTAGGTC
GGGGAGAGGGTAAGGGAATAGATCACTCAGATGTATTTTAGATAAGCTATTTAGCCTTTGATGGAATCATAAATACAGTGAATACAATCCTTTGCATTGT
TAAGGAGGTTTTTTGTTTTTAAATGGTGGGTCAAGGAGCTAGTTTACAGGCTTACTGTGATTTAAGCAAATGTGAAAAGTGAAACCTTAATTTTATCAAA
AGAAATTTCTGTAAATGGTATGTCTCCTTAGAATACCCAAATCATAATTTTATTTGTACACACTGTTAGGGGCTCATCTCATGTAGGCAGAGTATAAAGT
ATTACCTTTTGGAATTAAAAGCCACTGACTGTTATAAAGTATAACAACACACATCAGGTTTTAAAAAGCCTTGAATGGCCCTTGTCTTAAAAAGAAATTA
GGAGCCAGGTGCGGTGGCACGTGCCTGTAATCCCAGCTCCTTGGGAGGCTAAGACAGGAGGATTCCTTGAGCCCTGGAGTTTGAGTCCAGCCTGGGTGAC
ATAGCAAGACCCTGTCTTAAAAGAAAAATGGGAAGAAAGACAAGGTAACATGAAGAAAGAAGAGATACCTAGTATGATGGAGCTGCAAATTTCATGGCAG
TTCATGCAGTCGGTCAAGAGGAGGATTTTGTTTTGTAGTTTGCAGATGAGCATTTCTAAAGCATTTTCCCTTGCTGTATTTTTTTGTATTATAAATTACA
TTGGACTTCATATATATAATTTTTTTTTACATTATATGTCTCTTGTATGTTTTGAAACTCTTGTATTTATGATATAGCTTATATGATTTTTTTGCCTTGG
TATACATTTTAAAATATGAATTTAAAAAATTTTTGTAAAAATAAAATTCACAAAATTGTTTTGAAAAAC
  • APAF1-3 (forward primer)
  • APAF1-4 (reverse primer)
  • Single exon skipping

Frame 0

MSRGRSARRQRWPPLGAWGVFICISGRAPSGLG
GSGGI*LLRCPEAEKKR*RVDVTALSRAKGIEPE
VGSLGSRRPAKT*GAAAASGVAPGSLRGAAAVG
GRAPQAGKTSSLCVQ*WGPPEGGPWAGPHRGAP
GLWGQAALGGRPPRQPSEVPGGLRAPRGCRDPGE
APVRPGPAPGRRVCGETEPCTPNSRWKTPVAVS
LHRPGVSQSCPGSAALPTKT*AQRLGSWLTAQR
EKDLREDGCKSSKLFAST*RSSGKGHQDILHHGS
HD**WIFNNIRRGKSKK*AHSTAKSSYAD*NDT
*KR**FLRIILQCSTT*RI*RSCCPSP*WHSCC
LFFQCKDSPV*RWSTTEASCFCHKEEAGECNSAE
ALQIER*TRMGHHTWNGRLWEVCISCRSC*RSF
PFRRLFPRGSALGFSWETRQIWASDETAESLHT
VGSG*EFFPEAST*Y*RG*RPSPHSDASQTPKVS
LDLG*CLGLLGVESF*QSVSDSSYNQRQECYRF
SNGS*ICSPCGEFLRKGKRT*NFIPFC*YEEGR
FARTSS*YYKRM*RLSPCSIFNWCTFT*FSQSLG
VLPQTASE*AV*ENKEIFVL*L*GSR*SHVYKC
*NAQRRHQRLLHRSFHPSEGR*GAYKGVMYSLG
HGN*RS*RHTAGVCK*VSFIL*SEWKVVSLLFT*
SSSRFSYREELQPASGST*EDNHSVSEISPAAY
SFTRSGRLYVLVQLSGLSHGQCQDAQGTLCFNV
FPGLD*SKNRTCRPCSSDS*ICGIQTYTR*KGLC
SQ*EFSGVFIFKWTPSWTTAIS*YCTTGSL*AG
NFRSLSAS*AAGQAGGR*WNALPGMDKQKKHHE
SFPLSCPPPHRCCLPCLLF*GWSENSFLWS**NL
TGVQS*NRRETSRNQGS*G*SALLCILYR*QIY
SNLLSG*KSEDLEFYDWGTSTHL**ALRASQLL
PFHQQ*SSSSLSHWVK*LLPQTLGFESKRMSKYH
VWSYKFSQSLQIFTR**AFG*LFS*WNLKALGC
DISK*EEKH*CETVLPKFGGPSRGYGSDSEVLF
VVC*WCKDNGGSKK*NLFVEYRLTFKGG*LQRTF
KLGSWCDVFS*WIIIFDIF**PDNQALGDKESM
*ELCCNVKARSRCCVSRK*SDGPCS*PYKTSAT
H*WKNRSD*LSD*SSS*LLLLKSTSSVHCIWR*K
WSH*DFRTCKQ*NLPVQVSAQENCMAHPVHSR*
EDSYFKF**C*NSGMELAIGQMYLSTRPSGNSE
RL*TLEKFKTAFLVI*WNSEGMEYYYWK*RKRLC
LSPGYSTFL*HFSRCYQVFIYLC*QDCKDLEF*
SPFATS*IEGPQRLCALLCLLCGQYPAGNGR*Q
WRNQDMECLKR*ASSFVCSAFRRRSCYPWRLGD*
PLLFSRWQNAYLCWRIY*VVERCHWGILTDLLH
KWNQS*ENTRVP*LQNICDCG*SWYFIYFTDFR
IK*LSINVVELFKFLNWKKILMKP*YQLFIKLLI
VVQYCIHYKSVCGWMNNINVAFSQMNIPLILFF
MIIINSLSLGCK*KCEYIPCCTVGKILS*CIQN
G*HN**EEFGRNWYFNTVCIYYCYAGCASG*QWP
AF*TTLTPRGFCSPKYNLRGFLHSLNLL*KYCV
CVHSLQHFL*LTQ*VSLGFSRPPHLFFLFLETE
SCFVARLECSGAISAHHNRCLLGSSNSPASASRV
AGTTGVRTCQANFCIFSRDGVSPCWPGWSRSLD
LMIYPPWPPKVLRLQA*ATVPGQAPSLLMETGS
CTITQAGVQWHNHTSLQPQTPGFKQSSCLSLPSS
*DYRHEPPHPANF*VFL*RQGLTMLSRLVLNSW
PQVILLPQPPKVLGLQI*ATGLAFSSSFCEVKL
VCWKE*ILLVYPFLTVAAGSPVPYLDSSCQYLSW
TLFLLPLVSYISDFLLE*NLIFPNPHFFLF*KA
VSPLLNVSH*GGANFNHKP**DFSKKCEIEQFS
SNSIYF*MNGIVNAILLMNFKRILWFSV*FQMSH
CNSRRLTFQPTYFPFPCLSHPLFLPSFLSLLLS
PRLIRKNSF*QGKKLNN*LFLTS*SGTLVEA*I
*RNIFSEIYY*GQWR*INSRCGSQKI*SKFKDFF
CFCNWN*IK*LLVLIVDNLFLLLVHISITVG*V
GERVRE*ITQMYFR*AI*PLMES*IQ*IQSFAL
LRRFFVFKWWVKELVYRLTVI*ANVKSETLILSK
EISVNGMSP*NTQIIILFVHTVRGSSHVGRV*S
ITFWN*KPLTVIKYNNTHQVLKSLEWPLS*KEI
RSQVRWHVPVIPAPWEAKTGGFLEPWSLSPAWVT
*QDPVLKEKWEERQGNMKKEEIPSMMELQISWQ
FMQSVKRRILFCSLQMSISKAFSLAVFFCIINY
IGLHIYNFFLHYMSLVCFETLVFMI*LI*FFCLG
IHFKI*I*KIFVKIKFTKLF*K

Frame 1

*AVAGVRGGSGGRPLGLGVCLFA*AGARRPGWV
DPAGFDCSAVQRRRRRGSEWT*LLYPGQKG*NQ
RWGVWAVGDPRRLEVPQRHPE*RRAPSGVQPPSG
EGRHRPGRPPPFVSSSGVHRRAARGPGLTAALR
DCGVRLRWVDAHLANLRRSLGVFVRPGAAEIQG
RRL*GPDLPRGEGYVARQSPAPLIPGGKLLLPFP
STGLESPSLVPAVPPSPLRPRRKGLAHG*QLRE
RKI*GKMDAKARNCLLQHREALEKDIKTSYIMD
HMISDGFLTISEEEKVRNEPTQQQRAAMLIKMIL
KKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
SSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQ
KLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHS
LLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTR
LDQDESFSQRLPLNIEEAKDRLRILMLRKHPRS
LLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDS
VMGPKYVVPVESSLGKEKGLEILSLFVNMKKAD
LPEQAHSIIKECKGSPLVVSLIGALLRDFPNRW
EYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISV
EMLREDIKDYYTDLSILQKDVKVPTKVLCILWD
METEEVEDILQEFVNKSLLFCDRNGKSFRYYLH
DLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHT
LSPDQEDCMYWYNFLAYHMASAKMHKELCALMF
SLDWIKAKTELVGPAHLIHEFVEYRHILDEKDC
AVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPE
TSEVYQQAKLQAKQEVDNGMLYLEWINKKNITN
LSRLVVRPHTDAVYHACFSEDGQRIASCGADKT
LQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFI
ATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCC
HFTNSSHHLLLATGSSDCFLKLWDLNQKECRNT
MFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDA
TSANERKSINVKQFFLNLEDPQEDMEVIVKCCS
WSADGARIMVAAKNKIFLWNTDSRSKVADCRGH
LSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVC
KNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQL
INGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDE
NGAIEILELVNNRIFQSRFQHKKTVWHIQFTADE
KTLISSSDDAEIQVWNWQLDKCIFLRGHQETVK
DFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDF
VCHQGTVLSCDISHDATKFSSTSADKTAKIWSFD
LLLPLHELRGHNGCVRCSAFSVDSTLLATGDDN
GEIRIWNVSNGELLHLCAPLSEEGAATHGGWVT
DLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYT
NGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE
*NS*ALM*LNFLNF*IGKKF**NPDINFL*SS*
LLCSIAFITKVFVVG*IILM*LFPK*TYL*SCFS
*SSLTVCP*DANENVNTYLVVLLVKFCLDAFKM
VDIINEKNLEEIGILILSVFITVMQAVPQGSSG
LLFEPHLPQGGFVLLNTILEVFCTL*ICFKNIVS
VCIVCSISFN*LNK*VLDLAGPPTFFFCFWRQS
LALLPGWSAVARSRLTTIAASWVQAILLPQPPE
*LGLQVCAHARLIFVFLVETGFHHVGRDGLDLLT
S*STRLGLPKC*DYRREPPCLARPLLF*WRQGL
ALSPRLECSGIIIPHCSLRLLGSSNPPASASQV
AETTGTSHHTQLIFKFSCRDRVSLCCLGWS*TLG
LK*SSCLSLPKCWDCRYEPLAWPSAVLFVK*NL
YVGKSRFYWSTLFSL*LLAALCHIWTLVVSI*V
GHYSCSLLFLTYQTSYLNET*SFLILTFFFFKKQ
FLHC*MLVIEVGPILIISLNKIFLRNVK*NNFH
LIPFTFR*MAL*MPFF**ISREFSGFLCNSR*V
TVTLED*PSSQPIFLSLVSLILFSFLLSFLFFYL
QG*SGKIAFDRGKNSITSYF*PPDQEL*LKRKS
KETFSLKYIIKGNGDKLIVDVVPRKYNQNSKIF
FVSVTGTKSNDY*C***ITCFYCWCILV*LWGRS
GRG*GNRSLRCILDKLFSL*WNHKYSEYNPLHC
*GGFLFLNGGSRS*FTGLL*FKQM*KVKP*FYQ
KKFL*MVCLLRIPKS*FYLYTLLGAHLM*AEYKV
LPFGIKSH*LL*SITTHIRF*KALNGPCLKKKL
GARCGGTCL*SQLLGRLRQEDSLSPGV*VQPG*
HSKTLS*KKNGKKDKVT*RKKRYLV*WSCKFHGS
SCSRSRGGFCFVVCR*AFLKHFPLLYFFVL*IT
LDFIYIIFFYIICLLYVLKLLYL*YSLYDFFAL
VYILKYEFKKFL*K*NSQNCFEK

Frame 2

EPWQECAAAAVAAPWGLGCVYLHKRARAVRAGW
IRRDLTAPLSRGGEEEVASGRDCSIPGKRDRTR
GGESGQSATREDLRCRSGIRSSAGLPPGCSRRR
GKGATGREDLLPLCPVVGSTGGRPVGRASPRRSG
TVGSGCVGWTPTSPTFGGPWGSSCAPGLQRSRG
GACEARTCPGAKGMWRDRALHP*FPVENSCCRF
PPPAWSLPVLSRQCRPPH*DLGAKAWLMVDSSER
ERSEGRWMQKLEIVCFNIEKLWKRTSRHPTSWI
T*LVMDF*QYQKRKK*EMSPLNSKEQLC*LK*Y
LKKIMIPTYHSTMLYYMKDIKILLPFSMMAFLLS
LLPV*GQSCVKVEYHRGQLFLSQGRSW*MQFSR
SSPN*KVNQDGSPYMEWQAVGSLY*LQKLLEII
PF*KVVSQGECIGFQLGNKTNLGF**NCRIFAHG
WIRMRVFPRGFHLILKRLKTVSAF*CFANTQGL
S*SWMMFGTLGC*KLLTVSVRFFLQPETRVLQI
Q*WVLNM*SLWRVP*ERKKDLKFYPFLLI*RRQI
CQNKLIVL*KNVKALPL*YL*LVHFYVIFPIAG
STTSNSFRISSLRE*GNLRLMIMRL*MKPCL*V
LKCSEKTSKIITQIFPSFRRTLRCLQRCYVFSGT
WKLKKLKTYCRSL*ISLFYSVIGMESRFVIIYM
IFK*IFLQRRIAASFRIYIRR*SLSFRDITSRI
LFHQIRKTVCIGTTFWPITWPVPRCTRNFVL*CF
PWIGLKQKQNL*ALLI*FMNLWNTDIY*MKRIV
QSVRIFRSFYL*MDTFLDDSHFLILYNWVSVSR
KLQKFISKLSCRPSRRSIMECFTWNG*TKKTSRI
FPA*LSAPTQMLFTMPAFLRMVRE*LLVELIKP
YRCSKLKQERNF*KSRLMRMKCFVVHSLQMTDL
*QPAQWIKK*RFGIL*LGN*YTPMMSTQSKSIAA
ISPTVVIIFS*PLGQVTASSNFGI*IKKNVEIP
CLVIQIQSITADFHQMISFWLVVQLMEP*SFGM
RHQQMRGKALM*NSSS*IWRTLKRIWK***SVVR
GLLMVQG*WWQQKIKSFCGIQTHVQRWLIAEDI
*VGFMV*CFLLMDHHF*HLLMTRQSGSGRQRKY
VRTLL*C*SKK*MLCFKKMK*WSLQLTI*DVCNS
LMEEQVRLII*LKLKLAAVA*VHIFSTLHLEMK
MEPLRF*NL*TIESSSPGFSTRKLYGTSSSQPM
RRLLFQVLMMLKFRYGIGNWTNVSFYEAIRKQ*K
TLDS*KIQDCFLGHLMEQ*RYGILLLEIKKKTL
SVTRVQYFLVTFLTMLPSFHLPLLTRLQRSGVL
ISFCHFMN*GATTAVCAALPSLWTVPCWQREMTM
EKSGYGMSQTVSFFICVLRFQKKELLPMEAG*L
TFAFLQMAKCLSLLEDILSGGTLSLGNPHRPST
QMEPILRKYTCPLTSKHM*LWIILVFYIFYRL*N
KIVKH*CS*TF*IFELEKNSNETLISTFYKALN
CCAVLHSLQKCLWLDE*Y*CSFFPNEHTFNLVF
HDHH*QFVLRMQMKM*IHTLLYCW*NSVLMHSKW
LT*LMRRIWKKLVF*YCLYLLLLCRLCLRVAVA
CFLNHTYPKGVLFS*IQS*RFFALFKFALKILC
LCA*SAAFPLIDSISESWI*QAPPPFFFVFGDRV
LLCCQAGVQWRDLGSPQSLPPGFKQFSCLSLPS
SWDYRCAHMPG*FLYF**RRGFTMLAGMVSIS*
PHDLPALASQSAEITGVSHRAWPGPFSFNGDRVL
HYHPGWSAVA*SYLIAASDSWVQAILLPQPPK*
LRLQARATTPS*FLSFLVETGSHYVV*AGLELL
ASSNPPASASQSVGIADMSHWPGLQQFFL*SKTC
MLERVDFIGLPFSHCSCWQPCAISGL*LSVSEL
DTIPAPSCFLHIRLLT*MKPDLS*SSLFSFLKS
SFSTAKC*SLRWGQF*S*ALIRFF*EM*NRTIFI
*FHLLLDEWHCECHSFNEFQENSLVFCVIPDES
L*L*KINLPANLFSFPLSLSSSFPSFFPFSSFI
SKVNQEK*LLTGEKTQ*LAIFDLLIRNFS*SVNL
KKHFL*NILLRAMEIN***MWFPENIIKIQRFF
LFL*LELNQMITSVNSR*LVFIVGAY*YNCGVG
RGEGKGIDHSDVF*ISYLAFDGIINTVNTILCIV
KEVFCF*MVGQGASLQAYCDLSKCEK*NLNFIK
RNFCKWYVSLEYPNHNFICTHC*GLISCRQSIK
YYLLELKATDCYKV*QHTSGFKKP*MALVLKRN*
EPGAVARACNPSSLGG*DRRIP*ALEFESSLGD
IARPCLKRKMGRKTR*HEERRDT*YDGAANFMA
VHAVGQEEDFVL*FADEHF*SIFPCCIFLYYKLH
WTSYI*FFFTLYVSCMF*NSCIYDIAYMIFLPW
YTF*NMNLKNFCKNKIHKIVLKN

Sequence APAF1.cAug05

ATGAGCCGTGGCAGGAGTGCGCGGCGGCAGCGGTGGCCGCCCCTTGGGGCTTGGGGTGTGTTTATTTGCATAAGCGGGCGCGCGCCGTCCGGGCTGGGTG
GATCCGGCGGGATTTGACTGCTCCGCTGTCCAGAGGCGGAGAAGAAGAGGTAGCGAGTGGACGTGACTGCTCTATCCCGGGCAAAAGGGATAGAACCAGA
GGTGGGGAGTCTGGGCAGTCGGCGACCCGCGAAGACTTGAGGTGCCGCAGCGGCATCCGGAGTAGCGCCGGGCTCCCTCCGGGGTGCAGCCGCCGTCGGG
GGAAGGGCGCCACAGGCCGGGAAGACCTCCTCCCTTTGTGTCCAGTAGTGGGGTCCACCGGAGGGCGGCCCGTGGGCCGGGCCTCACCGCGGCGCTCCGG
GACTGTGGGGTCAGGCTGCGTTGGGTGGACGCCCACCTCGCCAACCTTCGGAGGTCCCTGGGGGTCTTCGTGCGCCCCGGGGCTGCAGAGATCCAGGGGA
GGCGCCTGTGAGGCCCGGACCTGCCCCGGGGCGAAGGGTATGTGGCGAGACAGAGCCCTGCACCCCTAATTCCCGGTGGAAAACTCCTGTTGCCGTTTCC
CTCCACCGGCCTGGAGTCTCCCAGTCTTGTCCCGGCAGTGCCGCCCTCCCCACTAAGACCTAGGCGCAAAGGCTTGGCTCATGGTTGACAGCTCAGAGAG
AGAAAGATCTGAGGGAAGATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACATCATGGATC
ACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACT
TAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TCTTCTTCCAGTGGTAAAGATTCAGTTAGTGGAATAACTTCGTATGTAAGGACAGTCCTGTGTGAAGGTGGAGTACCACAGAGGCCAGTTGTTTTTGTCA
CAAGGAAGAAGCTGGTGAATGCAATTCAGCAGAAGCTCTCCAAATTGAAAGGTGAACCAGGATGGGTCACCATACATGGAATGGCAGGCTGTGGGAAGTC
TGTATTAGCTGCAGAAGCTGTTAGAGATCATTCCCTTTTAGAAGGTTGTTTCCCAGGGGGAGTGCATTGGGTTTCAGTTGGGAAACAAGACAAATCTGGG
CTTCTGATGAAACTGCAGAATCTTTGCACACGGTTGGATCAGGATGAGAGTTTTTCCCAGAGGCTTCCACTTAATATTGAAGAGGCTAAAGACCGTCTCC
GCATTCTGATGCTTCGCAAACACCCAAGGTCTCTCTTGATCTTGGATGATGTTTGGGACTCTTGGGTGTTGAAAGCTTTTGACAGTCAGTGTCAGATTCT
TCTTACAACCAGAGACAAGAGTGTTACAGATTCAGTAATGGGTCCTAAATATGTAGTCCCTGTGGAGAGTTCCTTAGGAAAGGAAAAAGGACTTGAAATT
TTATCCCTTTTTGTTAATATGAAGAAGGCAGATTTGCCAGAACAAGCTCATAGTATTATAAAAGAATGTAAAGGCTCTCCCCTTGTAGTATCTTTAATTG
GTGCACTTTTACGTGATTTTCCCAATCGCTGGGAGTACTACCTCAAACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAATCTTCGTCTTATGATTA
TGAGGCTCTAGATGAAGCCATGTCTATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGATTATTACACAGATCTTTCCATCCTTCAGAAGGACGTTAAG
GTGCCTACAAAGGTGTTATGTATTCTCTGGGACATGGAAACTGAAGAAGTTGAAGACATACTGCAGGAGTTTGTAAATAAGTCTCTTTTATTCTGTGATC
GGAATGGAAAGTCGTTTCGTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATTGCAGCCAGCTTCAGGATCTACATAAGAAGATAAT
CACTCAGTTTCAGAGATATCACCAGCCGCATACTCTTTCACCAGATCAGGAAGACTGTATGTATTGGTACAACTTTCTGGCCTATCACATGGCCAGTGCC
AAGATGCACAAGGAACTTTGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAACAGAACTTGTAGGCCCTGCTCATCTGATTCATGAATTTGTGG
AATACAGACATATACTAGATGAAAAGGATTGTGCAGTCAGTGAGAATTTTCAGGAGTTTTTATCTTTAAATGGACACCTTCTTGGACGACAGCCATTTCC
TAATATTGTACAACTGGGTCTCTGTGAGCCGGAAACTTCAGAAGTTTATCAGCAAGCTAAGCTGCAGGCCAAGCAGGAGGTCGATAATGGAATGCTTTAC
CTGGAATGGATAAACAAAAAAAACATCACGAATCTTTCCCGCTTAGTTGTCCGCCCCCACACAGATGCTGTTTACCATGCCTGCTTTTCTGAGGATGGTC
AGAGAATAGCTTCTTGTGGAGCTGATAAAACCTTACAGGTGTTCAAAGCTGAAACAGGAGAGAAACTTCTAGAAATCAAGGCTCATGAGGATGAAGTGCT
TTGTTGTGCATTCTCTACAGATGACAGATTTATAGCAACCTGCTCAGTGGATAAAAAAGTGAAGATTTGGAATTCTATGACTGGGGAACTAGTACACACC
TATGATGAGCACTCAGAGCAAGTCAATTGCTGCCATTTCACCAACAGTAGTCATCATCTTCTCTTAGCCACTGGGTCAAGTGACTGCTTCCTCAAACTTT
GGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCATACAAATTCAGTCAATCACTGCAGATTTTCACCAGATGATAAGCTTTTGGCTAGTTG
TTCAGCTGATGGAACCTTAAAGCTTTGGGATGCGACATCAGCAAATGAGAGGAAAAGCATTAATGTGAAACAGTTCTTCCTAAATTTGGAGGACCCTCAA
GAGGATATGGAAGTGATAGTGAAGTGTTGTTCGTGGTCTGCTGATGGTGCAAGGATAATGGTGGCAGCAAAAAATAAAATCTTTTTGTGGAATACAGACT
CACGTTCAAAGGTGGCTGATTGCAGAGGACATTTAAGTTGGGTTCATGGTGTGATGTTTTCTCCTGATGGATCATCATTTTTGACATCTTCTGATGACCA
GACAATCAGGCTCTGGGAGACAAAGAAAGTATGTAAGAACTCTGCTGTAATGTTAAAGCAAGAAGTAGATGTTGTGTTTCAAGAAAATGAAGTGATGGTC
CTTGCAGTTGACCATATAAGACGTCTGCAACTCATTAATGGAAGAACAGGTCAGATTGATTATCTGACTGAAGCTCAAGTTAGCTGCTGTTGCTTAAGTC
CACATCTTCAGTACATTGCATTTGGAGATGAAAATGGAGCCATTGAGATTTTAGAACTTGTAAACAATAGAATCTTCCAGTCCAGGTTTCAGCACAAGAA
AACTGTATGGCACATCCAGTTCACAGCCGATGAGAAGACTCTTATTTCAAGTTCTGATGATGCTGAAATTCAGGTATGGAATTGGCAATTGGACAAATGT
ATCTTTCTACGAGGCCATCAGGAAACAGTGAAAGACTTTAGACTCTTGAAAAATTCAAGACTGCTTTCTTGGTCATTTGATGGAACAGTGAAGGTATGGA
ATATTATTACTGGAAATAAAGAAAAAGACTTTGTCTGTCACCAGGGTACAGTACTTTCTTGTGACATTTCTCACGATGCTACCAAGTTTTCATCTACCTC
TGCTGACAAGACTGCAAAGATCTGGAGTTTTGATCTCCTTTTGCCACTTCATGAATTGAGGGGCCACAACGGCTGTGTGCGCTGCTCTGCCTTCTCTGTG
GACAGTACCCTGCTGGCAACGGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAAACGGTGAGCTTCTTCATTTGTGTGCTCCGCTTTCAGAAG
AAGGAGCTGCTACCCATGGAGGCTGGGTGACTGACCTTTGCTTTTCTCCAGATGGCAAAATGCTTATCTCTGCTGGAGGATATATTAAGTGGTGGAACGT
TGTCACTGGGGAATCCTCACAGACCTTCTACACAAATGGAACCAATCTTAAGAAAATACACGTGTCCCCTGACTTCAAAACATATGTGACTGTGGATAAT
CTTGGTATTTTATATATTTTACAGACTTTAGAATAAAATAGTTAAGCATTAATGTAGTTGAACTTTTTAAATTTTTGAATTGGAAAAAAATTCTAATGAA
ACCCTGATATCAACTTTTTATAAAGCTCTTAATTGTTGTGCAGTATTGCATTCATTACAAAAGTGTTTGTGGTTGGATGAATAATATTAATGTAGCTTTT
TCCCAAATGAACATACCTTTAATCTTGTTTTTCATGATCATCATTAACAGTTTGTCCTTAGGATGCAAATGAAAATGTGAATACATACCTTGTTGTACTG
TTGGTAAAATTCTGTCTTGATGCATTCAAAATGGTTGACATAATTAATGAGAAGAATTTGGAAGAAATTGGTATTTTAATACTGTCTGTATTTATTACTG
TTATGCAGGCTGTGCCTCAGGGTAGCAGTGGCCTGCTTTTTGAACCACACTTACCCCAAGGGGGTTTTGTTCTCCTAAATACAATCTTAGAGGTTTTTTG
CACTCTTTAAATTTGCTTTAAAAATATTGTGTCTGTGTGCATAGTCTGCAGCATTTCCTTTAATTGACTCAATAAGTGAGTCTTGGATTTAGCAGGCCCC
CCCACCTTTTTTTTTTGTTTTTGGAGACAGAGTCTTGCTTTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACCACAATCGCTGCCTCCTGGG
TTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGTGTGCGCACATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCA
CCATGTTGGCCGGGATGGTCTCGATCTCTTGACCTCATGATCTACCCGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACCGTGCCTGGCC
AGGCCCCTTCTCTTTTAATGGAGACAGGGTCTTGCACTATCACCCAGGCTGGAGTGCAGTGGCATAATCATACCTCATTGCAGCCTCAGACTCCTGGGTT
CAAGCAATCCTCCTGCCTCAGCCTCCCAAGTAGCTGAGACTACAGGCACGAGCCACCACACCCAGCTAATTTTTAAGTTTTCTTGTAGAGACAGGGTCTC
ACTATGTTGTCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTAATCCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTGCAGATATGAGCCACTGGCCTGG
CCTTCAGCAGTTCTTTTTGTGAAGTAAAACTTGTATGTTGGAAAGAGTAGATTTTATTGGTCTACCCTTTTCTCACTGTAGCTGCTGGCAGCCCTGTGCC
ATATCTGGACTCTAGTTGTCAGTATCTGAGTTGGACACTATTCCTGCTCCCTCTTGTTTCTTACATATCAGACTTCTTACTTGAATGAAACCTGATCTTT
CCTAATCCTCACTTTTTTCTTTTTTAAAAAGCAGTTTCTCCACTGCTAAATGTTAGTCATTGAGGTGGGGCCAATTTTAATCATAAGCCTTAATAAGATT
TTTCTAAGAAATGTGAAATAGAACAATTTTCATCTAATTCCATTTACTTTTAGATGAATGGCATTGTGAATGCCATTCTTTTAATGAATTTCAAGAGAAT
TCTCTGGTTTTCTGTGTAATTCCAGATGAGTCACTGTAACTCTAGAAGATTAACCTTCCAGCCAACCTATTTTCCTTTCCCTTGTCTCTCTCATCCTCTT
TTCCTTCCTTCTTTCCTTTCTCTTCTTTTATCTCCAAGGTTAATCAGGAAAAATAGCTTTTGACAGGGGAAAAAACTCAATAACTAGCTATTTTTGACCT
CCTGATCAGGAACTTTAGTTGAAGCGTAAATCTAAAGAAACATTTTCTCTGAAATATATTATTAAGGGCAATGGAGATAAATTAATAGTAGATGTGGTTC
CCAGAAAATATAATCAAAATTCAAAGATTTTTTTTGTTTCTGTAACTGGAACTAAATCAAATGATTACTAGTGTTAATAGTAGATAACTTGTTTTTATTG
TTGGTGCATATTAGTATAACTGTGGGGTAGGTCGGGGAGAGGGTAAGGGAATAGATCACTCAGATGTATTTTAGATAAGCTATTTAGCCTTTGATGGAAT
CATAAATACAGTGAATACAATCCTTTGCATTGTTAAGGAGGTTTTTTGTTTTTAAATGGTGGGTCAAGGAGCTAGTTTACAGGCTTACTGTGATTTAAGC
AAATGTGAAAAGTGAAACCTTAATTTTATCAAAAGAAATTTCTGTAAATGGTATGTCTCCTTAGAATACCCAAATCATAATTTTATTTGTACACACTGTT
AGGGGCTCATCTCATGTAGGCAGAGTATAAAGTATTACCTTTTGGAATTAAAAGCCACTGACTGTTATAAAGTATAACAACACACATCAGGTTTTAAAAA
GCCTTGAATGGCCCTTGTCTTAAAAAGAAATTAGGAGCCAGGTGCGGTGGCACGTGCCTGTAATCCCAGCTCCTTGGGAGGCTAAGACAGGAGGATTCCT
TGAGCCCTGGAGTTTGAGTCCAGCCTGGGTGACATAGCAAGACCCTGTCTTAAAAGAAAAATGGGAAGAAAGACAAGGTAACATGAAGAAAGAAGAGATA
CCTAGTATGATGGAGCTGCAAATTTCATGGCAGTTCATGCAGTCGGTCAAGAGGAGGATTTTGTTTTGTAGTTTGCAGATGAGCATTTCTAAAGCATTTT
CCCTTGCTGTATTTTTTTGTATTATAAATTACATTGGACTTCATATATATAATTTTTTTTTACATTATATGTCTCTTGTATGTTTTGAAACTCTTGTATT
TATGATATAGCTTATATGATTTTTTTGCCTTGGTATACATTTTAAAATATGAATTTAAAAAATTTTTGTAAAAATAAAATTCACAAAATTGTTTTGAAAA
AC
  • APAF1-3 (forward primer)
  • APAF1-4 (reverse primer)
  • Single exon skipping

Frame 0

MSRGRSARRQRWPPLGAWGVFICISGRAPSGLG
GSGGI*LLRCPEAEKKR*RVDVTALSRAKGIEPE
VGSLGSRRPAKT*GAAAASGVAPGSLRGAAAVG
GRAPQAGKTSSLCVQ*WGPPEGGPWAGPHRGAP
GLWGQAALGGRPPRQPSEVPGGLRAPRGCRDPGE
APVRPGPAPGRRVCGETEPCTPNSRWKTPVAVS
LHRPGVSQSCPGSAALPTKT*AQRLGSWLTAQR
EKDLREDGCKSSKLFAST*RSSGKGHQDILHHGS
HD**WIFNNIRRGKSKK*AHSTAKSSYAD*NDT
*KR**FLRIILQCSTT*RI*RSCCPSP*WHSCC
LFFQW*RFS*WNNFVCKDSPV*RWSTTEASCFCH
KEEAGECNSAEALQIER*TRMGHHTWNGRLWEV
CISCRSC*RSFPFRRLFPRGSALGFSWETRQIW
ASDETAESLHTVGSG*EFFPEAST*Y*RG*RPSP
HSDASQTPKVSLDLG*CLGLLGVESF*QSVSDS
SYNQRQECYRFSNGS*ICSPCGEFLRKGKRT*N
FIPFC*YEEGRFARTSS*YYKRM*RLSPCSIFNW
CTFT*FSQSLGVLPQTASE*AV*ENKEIFVL*L
*GSR*SHVYKC*NAQRRHQRLLHRSFHPSEGR*
GAYKGVMYSLGHGN*RS*RHTAGVCK*VSFIL*S
EWKVVSLLFT*SSSRFSYREELQPASGST*EDN
HSVSEISPAAYSFTRSGRLYVLVQLSGLSHGQC
QDAQGTLCFNVFPGLD*SKNRTCRPCSSDS*ICG
IQTYTR*KGLCSQ*EFSGVFIFKWTPSWTTAIS
*YCTTGSL*AGNFRSLSAS*AAGQAGGR*WNAL
PGMDKQKKHHESFPLSCPPPHRCCLPCLLF*GWS
ENSFLWS**NLTGVQS*NRRETSRNQGS*G*SA
LLCILYR*QIYSNLLSG*KSEDLEFYDWGTSTH
L**ALRASQLLPFHQQ*SSSSLSHWVK*LLPQTL
GFESKRMSKYHVWSYKFSQSLQIFTR**AFG*L
FS*WNLKALGCDISK*EEKH*CETVLPKFGGPS
RGYGSDSEVLFVVC*WCKDNGGSKK*NLFVEYRL
TFKGG*LQRTFKLGSWCDVFS*WIIIFDIF**P
DNQALGDKESM*ELCCNVKARSRCCVSRK*SDG
PCS*PYKTSATH*WKNRSD*LSD*SSS*LLLLKS
TSSVHCIWR*KWSH*DFRTCKQ*NLPVQVSAQE
NCMAHPVHSR*EDSYFKF**C*NSGMELAIGQM
YLSTRPSGNSERL*TLEKFKTAFLVI*WNSEGME
YYYWK*RKRLCLSPGYSTFL*HFSRCYQVFIYL
C*QDCKDLEF*SPFATS*IEGPQRLCALLCLLC
GQYPAGNGR*QWRNQDMECLKR*ASSFVCSAFRR
RSCYPWRLGD*PLLFSRWQNAYLCWRIY*VVER
CHWGILTDLLHKWNQS*ENTRVP*LQNICDCG*
SWYFIYFTDFRIK*LSINVVELFKFLNWKKILMK
P*YQLFIKLLIVVQYCIHYKSVCGWMNNINVAF
SQMNIPLILFFMIIINSLSLGCK*KCEYIPCCT
VGKILS*CIQNG*HN**EEFGRNWYFNTVCIYYC
YAGCASG*QWPAF*TTLTPRGFCSPKYNLRGFL
HSLNLL*KYCVCVHSLQHFL*LTQ*VSLGFSRP
PHLFFLFLETESCFVARLECSGAISAHHNRCLLG
SSNSPASASRVAGTTGVRTCQANFCIFSRDGVS
PCWPGWSRSLDLMIYPPWPPKVLRLQA*ATVPG
QAPSLLMETGSCTITQAGVQWHNHTSLQPQTPGF
KQSSCLSLPSS*DYRHEPPHPANF*VFL*RQGL
TMLSRLVLNSWPQVILLPQPPKVLGLQI*ATGL
AFSSSFCEVKLVCWKE*ILLVYPFLTVAAGSPVP
YLDSSCQYLSWTLFLLPLVSYISDFLLE*NLIF
PNPHFFLF*KAVSPLLNVSH*GGANFNHKP**D
FSKKCEIEQFSSNSIYF*MNGIVNAILLMNFKRI
LWFSV*FQMSHCNSRRLTFQPTYFPFPCLSHPL
FLPSFLSLLLSPRLIRKNSF*QGKKLNN*LFLT
S*SGTLVEA*I*RNIFSEIYY*GQWR*INSRCGS
QKI*SKFKDFFCFCNWN*IK*LLVLIVDNLFLL
LVHISITVG*VGERVRE*ITQMYFR*AI*PLME
S*IQ*IQSFALLRRFFVFKWWVKELVYRLTVI*A
NVKSETLILSKEISVNGMSP*NTQIIILFVHTV
RGSSHVGRV*SITFWN*KPLTVIKYNNTHQVLK
SLEWPLS*KEIRSQVRWHVPVIPAPWEAKTGGFL
EPWSLSPAWVT*QDPVLKEKWEERQGNMKKEEI
PSMMELQISWQFMQSVKRRILFCSLQMSISKAF
SLAVFFCIINYIGLHIYNFFLHYMSLVCFETLVF
MI*LI*FFCLGIHFKI*I*KIFVKIKFTKLF*K

Frame 1

*AVAGVRGGSGGRPLGLGVCLFA*AGARRPGWV
DPAGFDCSAVQRRRRRGSEWT*LLYPGQKG*NQ
RWGVWAVGDPRRLEVPQRHPE*RRAPSGVQPPSG
EGRHRPGRPPPFVSSSGVHRRAARGPGLTAALR
DCGVRLRWVDAHLANLRRSLGVFVRPGAAEIQG
RRL*GPDLPRGEGYVARQSPAPLIPGGKLLLPFP
STGLESPSLVPAVPPSPLRPRRKGLAHG*QLRE
RKI*GKMDAKARNCLLQHREALEKDIKTSYIMD
HMISDGFLTISEEEKVRNEPTQQQRAAMLIKMIL
KKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
SSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFV
TRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKS
VLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSG
LLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRL
RILMLRKHPRSLLILDDVWDSWVLKAFDSQCQIL
LTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEI
LSLFVNMKKADLPEQAHSIIKECKGSPLVVSLI
GALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDY
EALDEAMSISVEMLREDIKDYYTDLSILQKDVK
VPTKVLCILWDMETEEVEDILQEFVNKSLLFCD
RNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKII
TQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASA
KMHKELCALMFSLDWIKAKTELVGPAHLIHEFV
EYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFP
NIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLY
LEWINKKNITNLSRLVVRPHTDAVYHACFSEDG
QRIASCGADKTLQVFKAETGEKLLEIKAHEDEVL
CCAFSTDDRFIATCSVDKKVKIWNSMTGELVHT
YDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKL
WDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASC
SADGTLKLWDATSANERKSINVKQFFLNLEDPQ
EDMEVIVKCCSWSADGARIMVAAKNKIFLWNTD
SRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQ
TIRLWETKKVCKNSAVMLKQEVDVVFQENEVMV
LAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLS
PHLQYIAFGDENGAIEILELVNNRIFQSRFQHKK
TVWHIQFTADEKTLISSSDDAEIQVWNWQLDKC
IFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVW
NIITGNKEKDFVCHQGTVLSCDISHDATKFSSTS
ADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSV
DSTLLATGDDNGEIRIWNVSNGELLHLCAPLSE
EGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNV
VTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDN
LGILYILQTLE*NS*ALM*LNFLNF*IGKKF**
NPDINFL*SS*LLCSIAFITKVFVVG*IILM*LF
PK*TYL*SCFS*SSLTVCP*DANENVNTYLVVL
LVKFCLDAFKMVDIINEKNLEEIGILILSVFIT
VMQAVPQGSSGLLFEPHLPQGGFVLLNTILEVFC
TL*ICFKNIVSVCIVCSISFN*LNK*VLDLAGP
PTFFFCFWRQSLALLPGWSAVARSRLTTIAASW
VQAILLPQPPE*LGLQVCAHARLIFVFLVETGFH
HVGRDGLDLLTS*STRLGLPKC*DYRREPPCLA
RPLLF*WRQGLALSPRLECSGIIIPHCSLRLLG
SSNPPASASQVAETTGTSHHTQLIFKFSCRDRVS
LCCLGWS*TLGLK*SSCLSLPKCWDCRYEPLAW
PSAVLFVK*NLYVGKSRFYWSTLFSL*LLAALC
HIWTLVVSI*VGHYSCSLLFLTYQTSYLNET*SF
LILTFFFFKKQFLHC*MLVIEVGPILIISLNKI
FLRNVK*NNFHLIPFTFR*MAL*MPFF**ISRE
FSGFLCNSR*VTVTLED*PSSQPIFLSLVSLILF
SFLLSFLFFYLQG*SGKIAFDRGKNSITSYF*P
PDQEL*LKRKSKETFSLKYIIKGNGDKLIVDVV
PRKYNQNSKIFFVSVTGTKSNDY*C***ITCFYC
WCILV*LWGRSGRG*GNRSLRCILDKLFSL*WN
HKYSEYNPLHC*GGFLFLNGGSRS*FTGLL*FK
QM*KVKP*FYQKKFL*MVCLLRIPKS*FYLYTLL
GAHLM*AEYKVLPFGIKSH*LL*SITTHIRF*K
ALNGPCLKKKLGARCGGTCL*SQLLGRLRQEDS
LSPGV*VQPG*HSKTLS*KKNGKKDKVT*RKKRY
LV*WSCKFHGSSCSRSRGGFCFVVCR*AFLKHF
PLLYFFVL*ITLDFIYIIFFYIICLLYVLKLLY
L*YSLYDFFALVYILKYEFKKFL*K*NSQNCFEK

Frame 2

EPWQECAAAAVAAPWGLGCVYLHKRARAVRAGW
IRRDLTAPLSRGGEEEVASGRDCSIPGKRDRTR
GGESGQSATREDLRCRSGIRSSAGLPPGCSRRR
GKGATGREDLLPLCPVVGSTGGRPVGRASPRRSG
TVGSGCVGWTPTSPTFGGPWGSSCAPGLQRSRG
GACEARTCPGAKGMWRDRALHP*FPVENSCCRF
PPPAWSLPVLSRQCRPPH*DLGAKAWLMVDSSER
ERSEGRWMQKLEIVCFNIEKLWKRTSRHPTSWI
T*LVMDF*QYQKRKK*EMSPLNSKEQLC*LK*Y
LKKIMIPTYHSTMLYYMKDIKILLPFSMMAFLLS
LLPVVKIQLVE*LRM*GQSCVKVEYHRGQLFLS
QGRSW*MQFSRSSPN*KVNQDGSPYMEWQAVGS
LY*LQKLLEIIPF*KVVSQGECIGFQLGNKTNLG
F**NCRIFAHGWIRMRVFPRGFHLILKRLKTVS
AF*CFANTQGLS*SWMMFGTLGC*KLLTVSVRF
FLQPETRVLQIQ*WVLNM*SLWRVP*ERKKDLKF
YPFLLI*RRQICQNKLIVL*KNVKALPL*YL*L
VHFYVIFPIAGSTTSNSFRISSLRE*GNLRLMI
MRL*MKPCL*VLKCSEKTSKIITQIFPSFRRTLR
CLQRCYVFSGTWKLKKLKTYCRSL*ISLFYSVI
GMESRFVIIYMIFK*IFLQRRIAASFRIYIRR*
SLSFRDITSRILFHQIRKTVCIGTTFWPITWPVP
RCTRNFVL*CFPWIGLKQKQNL*ALLI*FMNLW
NTDIY*MKRIVQSVRIFRSFYL*MDTFLDDSHF
LILYNWVSVSRKLQKFISKLSCRPSRRSIMECFT
WNG*TKKTSRIFPA*LSAPTQMLFTMPAFLRMV
RE*LLVELIKPYRCSKLKQERNF*KSRLMRMKC
FVVHSLQMTDL*QPAQWIKK*RFGIL*LGN*YTP
MMSTQSKSIAAISPTVVIIFS*PLGQVTASSNF
GI*IKKNVEIPCLVIQIQSITADFHQMISFWLV
VQLMEP*SFGMRHQQMRGKALM*NSSS*IWRTLK
RIWK***SVVRGLLMVQG*WWQQKIKSFCGIQT
HVQRWLIAEDI*VGFMV*CFLLMDHHF*HLLMT
RQSGSGRQRKYVRTLL*C*SKK*MLCFKKMK*WS
LQLTI*DVCNSLMEEQVRLII*LKLKLAAVA*V
HIFSTLHLEMKMEPLRF*NL*TIESSSPGFSTR
KLYGTSSSQPMRRLLFQVLMMLKFRYGIGNWTNV
SFYEAIRKQ*KTLDS*KIQDCFLGHLMEQ*RYG
ILLLEIKKKTLSVTRVQYFLVTFLTMLPSFHLP
LLTRLQRSGVLISFCHFMN*GATTAVCAALPSLW
TVPCWQREMTMEKSGYGMSQTVSFFICVLRFQK
KELLPMEAG*LTFAFLQMAKCLSLLEDILSGGT
LSLGNPHRPSTQMEPILRKYTCPLTSKHM*LWII
LVFYIFYRL*NKIVKH*CS*TF*IFELEKNSNE
TLISTFYKALNCCAVLHSLQKCLWLDE*Y*CSF
FPNEHTFNLVFHDHH*QFVLRMQMKM*IHTLLYC
W*NSVLMHSKWLT*LMRRIWKKLVF*YCLYLLL
LCRLCLRVAVACFLNHTYPKGVLFS*IQS*RFF
ALFKFALKILCLCA*SAAFPLIDSISESWI*QAP
PPFFFVFGDRVLLCCQAGVQWRDLGSPQSLPPG
FKQFSCLSLPSSWDYRCAHMPG*FLYF**RRGF
TMLAGMVSIS*PHDLPALASQSAEITGVSHRAWP
GPFSFNGDRVLHYHPGWSAVA*SYLIAASDSWV
QAILLPQPPK*LRLQARATTPS*FLSFLVETGS
HYVV*AGLELLASSNPPASASQSVGIADMSHWPG
LQQFFL*SKTCMLERVDFIGLPFSHCSCWQPCA
ISGL*LSVSELDTIPAPSCFLHIRLLT*MKPDL
S*SSLFSFLKSSFSTAKC*SLRWGQF*S*ALIRF
F*EM*NRTIFI*FHLLLDEWHCECHSFNEFQEN
SLVFCVIPDESL*L*KINLPANLFSFPLSLSSS
FPSFFPFSSFISKVNQEK*LLTGEKTQ*LAIFDL
LIRNFS*SVNLKKHFL*NILLRAMEIN***MWF
PENIIKIQRFFLFL*LELNQMITSVNSR*LVFI
VGAY*YNCGVGRGEGKGIDHSDVF*ISYLAFDGI
INTVNTILCIVKEVFCF*MVGQGASLQAYCDLS
KCEK*NLNFIKRNFCKWYVSLEYPNHNFICTHC
*GLISCRQSIKYYLLELKATDCYKV*QHTSGFKK
P*MALVLKRN*EPGAVARACNPSSLGG*DRRIP
*ALEFESSLGDIARPCLKRKMGRKTR*HEERRD
T*YDGAANFMAVHAVGQEEDFVL*FADEHF*SIF
PCCIFLYYKLHWTSYI*FFFTLYVSCMF*NSCI
YDIAYMIFLPWYTF*NMNLKNFCKNKIHKIVLK
N

Sequence APAF1.bAug05

ATGAGCCGTGGCAGGAGTGCGCGGCGGCAGCGGTGGCCGCCCCTTGGGGCTTGGGGTGTGTTTATTTGCATAAGCGGGCGCGCGCCGTCCGGGCTGGGTG
GATCCGGCGGGATTTGACTGCTCCGCTGTCCAGAGGCGGAGAAGAAGAGGTAGCGAGTGGACGTGACTGCTCTATCCCGGGCAAAAGGGATAGAACCAGA
GGTGGGGAGTCTGGGCAGTCGGCGACCCGCGAAGACTTGAGGTGCCGCAGCGGCATCCGGAGTAGCGCCGGGCTCCCTCCGGGGTGCAGCCGCCGTCGGG
GGAAGGGCGCCACAGGCCGGGAAGACCTCCTCCCTTTGTGTCCAGTAGTGGGGTCCACCGGAGGGCGGCCCGTGGGCCGGGCCTCACCGCGGCGCTCCGG
GACTGTGGGGTCAGGCTGCGTTGGGTGGACGCCCACCTCGCCAACCTTCGGAGGTCCCTGGGGGTCTTCGTGCGCCCCGGGGCTGCAGAGATCCAGGGGA
GGCGCCTGTGAGGCCCGGACCTGCCCCGGGGCGAAGGGTATGTGGCGAGACAGAGCCCTGCACCCCTAATTCCCGGTGGAAAACTCCTGTTGCCGTTTCC
CTCCACCGGCCTGGAGTCTCCCAGTCTTGTCCCGGCAGTGCCGCCCTCCCCACTAAGACCTAGGCGCAAAGGCTTGGCTCATGGTTGACAGCTCAGAGAG
AGAAAGATCTGAGGGAAGATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACATCATGGATC
ACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACT
TAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TCTTCTTCCAGTGTAAGGACAGTCCTGTGTGAAGGTGGAGTACCACAGAGGCCAGTTGTTTTTGTCACAAGGAAGAAGCTGGTGAATGCAATTCAGCAGA
AGCTCTCCAAATTGAAAGGTGAACCAGGATGGGTCACCATACATGGAATGGCAGGCTGTGGGAAGTCTGTATTAGCTGCAGAAGCTGTTAGAGATCATTC
CCTTTTAGAAGGTTGTTTCCCAGGGGGAGTGCATTGGGTTTCAGTTGGGAAACAAGACAAATCTGGGCTTCTGATGAAACTGCAGAATCTTTGCACACGG
TTGGATCAGGATGAGAGTTTTTCCCAGAGGCTTCCACTTAATATTGAAGAGGCTAAAGACCGTCTCCGCATTCTGATGCTTCGCAAACACCCAAGGTCTC
TCTTGATCTTGGATGATGTTTGGGACTCTTGGGTGTTGAAAGCTTTTGACAGTCAGTGTCAGATTCTTCTTACAACCAGAGACAAGAGTGTTACAGATTC
AGTAATGGGTCCTAAATATGTAGTCCCTGTGGAGAGTTCCTTAGGAAAGGAAAAAGGACTTGAAATTTTATCCCTTTTTGTTAATATGAAGAAGGCAGAT
TTGCCAGAACAAGCTCATAGTATTATAAAAGAATGTAAAGGCTCTCCCCTTGTAGTATCTTTAATTGGTGCACTTTTACGTGATTTTCCCAATCGCTGGG
AGTACTACCTCAAACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAATCTTCGTCTTATGATTATGAGGCTCTAGATGAAGCCATGTCTATAAGTGT
TGAAATGCTCAGAGAAGACATCAAAGATTATTACACAGATCTTTCCATCCTTCAGAAGGACGTTAAGGTGCCTACAAAGGTGTTATGTATTCTCTGGGAC
ATGGAAACTGAAGAAGTTGAAGACATACTGCAGGAGTTTGTAAATAAGTCTCTTTTATTCTGTGATCGGAATGGAAAGTCGTTTCGTTATTATTTACATG
ATCTTCAAGTAGATTTTCTTACAGAGAAGAATTGCAGCCAGCTTCAGGATCTACATAAGAAGATAATCACTCAGTTTCAGAGATATCACCAGCCGCATAC
TCTTTCACCAGATCAGGAAGACTGTATGTATTGGTACAACTTTCTGGCCTATCACATGGCCAGTGCCAAGATGCACAAGGAACTTTGTGCTTTAATGTTT
TCCCTGGATTGGATTAAAGCAAAAACAGAACTTGTAGGCCCTGCTCATCTGATTCATGAATTTGTGGAATACAGACATATACTAGATGAAAAGGATTGTG
CAGTCAGTGAGAATTTTCAGGAGTTTTTATCTTTAAATGGACACCTTCTTGGACGACAGCCATTTCCTAATATTGTACAACTGGGTCTCTGTGAGCCGGA
AACTTCAGAAGTTTATCAGCAAGCTAAGCTGCAGGCCAAGCAGGAGGTCGATAATGGAATGCTTTACCTGGAATGGATAAACAAAAAAAACATCACGAAT
CTTTCCCGCTTAGTTGTCCGCCCCCACACAGATGCTGTTTACCATGCCTGCTTTTCTGAGGATGGTCAGAGAATAGCTTCTTGTGGAGCTGATAAAACCT
TACAGGTGTTCAAAGCTGAAACAGGAGAGAAACTTCTAGAAATCAAGGCTCATGAGGATGAAGTGCTTTGTTGTGCATTCTCTACAGATGACAGATTTAT
AGCAACCTGCTCAGTGGATAAAAAAGTGAAGATTTGGAATTCTATGACTGGGGAACTAGTACACACCTATGATGAGCACTCAGAGCAAGTCAATTGCTGC
CATTTCACCAACAGTAGTCATCATCTTCTCTTAGCCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGGGATTTGAATCAAAAAGAATGTCGAAATACCA
TGTTTGGTCATACAAATTCAGTCAATCACTGCAGATTTTCACCAGATGATAAGCTTTTGGCTAGTTGTTCAGCTGATGGAACCTTAAAGCTTTGGGATGC
GACATCAGCAAATGAGAGGAAAAGCATTAATGTGAAACAGTTCTTCCTAAATTTGGAGGACCCTCAAGAGGATATGGAAGTGATAGTGAAGTGTTGTTCG
TGGTCTGCTGATGGTGCAAGGATAATGGTGGCAGCAAAAAATAAAATCTTTCTTTTTGACATTCATACTAGTGGCCTATTGGGAGAAATCCACACGGGCC
ATCACAGCACCATCCAGTACTGTGACTTCTCCCCACAAAACCATTTGGCAGTGGTTGCTTTGTCCCAGTACTGTGTAGAGTTGTGGAATACAGACTCACG
TTCAAAGGTGGCTGATTGCAGAGGACATTTAAGTTGGGTTCATGGTGTGATGTTTTCTCCTGATGGATCATCATTTTTGACATCTTCTGATGACCAGACA
ATCAGGCTCTGGGAGACAAAGAAAGTATGTAAGAACTCTGCTGTAATGTTAAAGCAAGAAGTAGATGTTGTGTTTCAAGAAAATGAAGTGATGGTCCTTG
CAGTTGACCATATAAGACGTCTGCAACTCATTAATGGAAGAACAGGTCAGATTGATTATCTGACTGAAGCTCAAGTTAGCTGCTGTTGCTTAAGTCCACA
TCTTCAGTACATTGCATTTGGAGATGAAAATGGAGCCATTGAGATTTTAGAACTTGTAAACAATAGAATCTTCCAGTCCAGGTTTCAGCACAAGAAAACT
GTATGGCACATCCAGTTCACAGCCGATGAGAAGACTCTTATTTCAAGTTCTGATGATGCTGAAATTCAGGTATGGAATTGGCAATTGGACAAATGTATCT
TTCTACGAGGCCATCAGGAAACAGTGAAAGACTTTAGACTCTTGAAAAATTCAAGACTGCTTTCTTGGTCATTTGATGGAACAGTGAAGGTATGGAATAT
TATTACTGGAAATAAAGAAAAAGACTTTGTCTGTCACCAGGGTACAGTACTTTCTTGTGACATTTCTCACGATGCTACCAAGTTTTCATCTACCTCTGCT
GACAAGACTGCAAAGATCTGGAGTTTTGATCTCCTTTTGCCACTTCATGAATTGAGGGGCCACAACGGCTGTGTGCGCTGCTCTGCCTTCTCTGTGGACA
GTACCCTGCTGGCAACGGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAAACGGTGAGCTTCTTCATTTGTGTGCTCCGCTTTCAGAAGAAGG
AGCTGCTACCCATGGAGGCTGGGTGACTGACCTTTGCTTTTCTCCAGATGGCAAAATGCTTATCTCTGCTGGAGGATATATTAAGTGGTGGAACGTTGTC
ACTGGGGAATCCTCACAGACCTTCTACACAAATGGAACCAATCTTAAGAAAATACACGTGTCCCCTGACTTCAAAACATATGTGACTGTGGATAATCTTG
GTATTTTATATATTTTACAGACTTTAGAATAAAATAGTTAAGCATTAATGTAGTTGAACTTTTTAAATTTTTGAATTGGAAAAAAATTCTAATGAAACCC
TGATATCAACTTTTTATAAAGCTCTTAATTGTTGTGCAGTATTGCATTCATTACAAAAGTGTTTGTGGTTGGATGAATAATATTAATGTAGCTTTTTCCC
AAATGAACATACCTTTAATCTTGTTTTTCATGATCATCATTAACAGTTTGTCCTTAGGATGCAAATGAAAATGTGAATACATACCTTGTTGTACTGTTGG
TAAAATTCTGTCTTGATGCATTCAAAATGGTTGACATAATTAATGAGAAGAATTTGGAAGAAATTGGTATTTTAATACTGTCTGTATTTATTACTGTTAT
GCAGGCTGTGCCTCAGGGTAGCAGTGGCCTGCTTTTTGAACCACACTTACCCCAAGGGGGTTTTGTTCTCCTAAATACAATCTTAGAGGTTTTTTGCACT
CTTTAAATTTGCTTTAAAAATATTGTGTCTGTGTGCATAGTCTGCAGCATTTCCTTTAATTGACTCAATAAGTGAGTCTTGGATTTAGCAGGCCCCCCCA
CCTTTTTTTTTTGTTTTTGGAGACAGAGTCTTGCTTTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACCACAATCGCTGCCTCCTGGGTTCA
AGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGTGTGCGCACATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCAT
GTTGGCCGGGATGGTCTCGATCTCTTGACCTCATGATCTACCCGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACCGTGCCTGGCCAGGC
CCCTTCTCTTTTAATGGAGACAGGGTCTTGCACTATCACCCAGGCTGGAGTGCAGTGGCATAATCATACCTCATTGCAGCCTCAGACTCCTGGGTTCAAG
CAATCCTCCTGCCTCAGCCTCCCAAGTAGCTGAGACTACAGGCACGAGCCACCACACCCAGCTAATTTTTAAGTTTTCTTGTAGAGACAGGGTCTCACTA
TGTTGTCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTAATCCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTGCAGATATGAGCCACTGGCCTGGCCTT
CAGCAGTTCTTTTTGTGAAGTAAAACTTGTATGTTGGAAAGAGTAGATTTTATTGGTCTACCCTTTTCTCACTGTAGCTGCTGGCAGCCCTGTGCCATAT
CTGGACTCTAGTTGTCAGTATCTGAGTTGGACACTATTCCTGCTCCCTCTTGTTTCTTACATATCAGACTTCTTACTTGAATGAAACCTGATCTTTCCTA
ATCCTCACTTTTTTCTTTTTTAAAAAGCAGTTTCTCCACTGCTAAATGTTAGTCATTGAGGTGGGGCCAATTTTAATCATAAGCCTTAATAAGATTTTTC
TAAGAAATGTGAAATAGAACAATTTTCATCTAATTCCATTTACTTTTAGATGAATGGCATTGTGAATGCCATTCTTTTAATGAATTTCAAGAGAATTCTC
TGGTTTTCTGTGTAATTCCAGATGAGTCACTGTAACTCTAGAAGATTAACCTTCCAGCCAACCTATTTTCCTTTCCCTTGTCTCTCTCATCCTCTTTTCC
TTCCTTCTTTCCTTTCTCTTCTTTTATCTCCAAGGTTAATCAGGAAAAATAGCTTTTGACAGGGGAAAAAACTCAATAACTAGCTATTTTTGACCTCCTG
ATCAGGAACTTTAGTTGAAGCGTAAATCTAAAGAAACATTTTCTCTGAAATATATTATTAAGGGCAATGGAGATAAATTAATAGTAGATGTGGTTCCCAG
AAAATATAATCAAAATTCAAAGATTTTTTTTGTTTCTGTAACTGGAACTAAATCAAATGATTACTAGTGTTAATAGTAGATAACTTGTTTTTATTGTTGG
TGCATATTAGTATAACTGTGGGGTAGGTCGGGGAGAGGGTAAGGGAATAGATCACTCAGATGTATTTTAGATAAGCTATTTAGCCTTTGATGGAATCATA
AATACAGTGAATACAATCCTTTGCATTGTTAAGGAGGTTTTTTGTTTTTAAATGGTGGGTCAAGGAGCTAGTTTACAGGCTTACTGTGATTTAAGCAAAT
GTGAAAAGTGAAACCTTAATTTTATCAAAAGAAATTTCTGTAAATGGTATGTCTCCTTAGAATACCCAAATCATAATTTTATTTGTACACACTGTTAGGG
GCTCATCTCATGTAGGCAGAGTATAAAGTATTACCTTTTGGAATTAAAAGCCACTGACTGTTATAAAGTATAACAACACACATCAGGTTTTAAAAAGCCT
TGAATGGCCCTTGTCTTAAAAAGAAATTAGGAGCCAGGTGCGGTGGCACGTGCCTGTAATCCCAGCTCCTTGGGAGGCTAAGACAGGAGGATTCCTTGAG
CCCTGGAGTTTGAGTCCAGCCTGGGTGACATAGCAAGACCCTGTCTTAAAAGAAAAATGGGAAGAAAGACAAGGTAACATGAAGAAAGAAGAGATACCTA
GTATGATGGAGCTGCAAATTTCATGGCAGTTCATGCAGTCGGTCAAGAGGAGGATTTTGTTTTGTAGTTTGCAGATGAGCATTTCTAAAGCATTTTCCCT
TGCTGTATTTTTTTGTATTATAAATTACATTGGACTTCATATATATAATTTTTTTTTACATTATATGTCTCTTGTATGTTTTGAAACTCTTGTATTTATG
ATATAGCTTATATGATTTTTTTGCCTTGGTATACATTTTAAAATATGAATTTAAAAAATTTTTGTAAAAATAAAATTCACAAAATTGTTTTGAAAAAC
  • APAF1-3 (forward primer)
  • APAF1-4 (reverse primer)
  • Single exon skipping

Frame 0

MSRGRSARRQRWPPLGAWGVFICISGRAPSGLG
GSGGI*LLRCPEAEKKR*RVDVTALSRAKGIEPE
VGSLGSRRPAKT*GAAAASGVAPGSLRGAAAVG
GRAPQAGKTSSLCVQ*WGPPEGGPWAGPHRGAP
GLWGQAALGGRPPRQPSEVPGGLRAPRGCRDPGE
APVRPGPAPGRRVCGETEPCTPNSRWKTPVAVS
LHRPGVSQSCPGSAALPTKT*AQRLGSWLTAQR
EKDLREDGCKSSKLFAST*RSSGKGHQDILHHGS
HD**WIFNNIRRGKSKK*AHSTAKSSYAD*NDT
*KR**FLRIILQCSTT*RI*RSCCPSP*WHSCC
LFFQCKDSPV*RWSTTEASCFCHKEEAGECNSAE
ALQIER*TRMGHHTWNGRLWEVCISCRSC*RSF
PFRRLFPRGSALGFSWETRQIWASDETAESLHT
VGSG*EFFPEAST*Y*RG*RPSPHSDASQTPKVS
LDLG*CLGLLGVESF*QSVSDSSYNQRQECYRF
SNGS*ICSPCGEFLRKGKRT*NFIPFC*YEEGR
FARTSS*YYKRM*RLSPCSIFNWCTFT*FSQSLG
VLPQTASE*AV*ENKEIFVL*L*GSR*SHVYKC
*NAQRRHQRLLHRSFHPSEGR*GAYKGVMYSLG
HGN*RS*RHTAGVCK*VSFIL*SEWKVVSLLFT*
SSSRFSYREELQPASGST*EDNHSVSEISPAAY
SFTRSGRLYVLVQLSGLSHGQCQDAQGTLCFNV
FPGLD*SKNRTCRPCSSDS*ICGIQTYTR*KGLC
SQ*EFSGVFIFKWTPSWTTAIS*YCTTGSL*AG
NFRSLSAS*AAGQAGGR*WNALPGMDKQKKHHE
SFPLSCPPPHRCCLPCLLF*GWSENSFLWS**NL
TGVQS*NRRETSRNQGS*G*SALLCILYR*QIY
SNLLSG*KSEDLEFYDWGTSTHL**ALRASQLL
PFHQQ*SSSSLSHWVK*LLPQTLGFESKRMSKYH
VWSYKFSQSLQIFTR**AFG*LFS*WNLKALGC
DISK*EEKH*CETVLPKFGGPSRGYGSDSEVLF
VVC*WCKDNGGSKK*NLSF*HSY*WPIGRNPHGP
SQHHPVL*LLPTKPFGSGCFVPVLCRVVEYRLT
FKGG*LQRTFKLGSWCDVFS*WIIIFDIF**PD
NQALGDKESM*ELCCNVKARSRCCVSRK*SDGPC
S*PYKTSATH*WKNRSD*LSD*SSS*LLLLKST
SSVHCIWR*KWSH*DFRTCKQ*NLPVQVSAQEN
CMAHPVHSR*EDSYFKF**C*NSGMELAIGQMYL
STRPSGNSERL*TLEKFKTAFLVI*WNSEGMEY
YYWK*RKRLCLSPGYSTFL*HFSRCYQVFIYLC
*QDCKDLEF*SPFATS*IEGPQRLCALLCLLCGQ
YPAGNGR*QWRNQDMECLKR*ASSFVCSAFRRR
SCYPWRLGD*PLLFSRWQNAYLCWRIY*VVERC
HWGILTDLLHKWNQS*ENTRVP*LQNICDCG*SW
YFIYFTDFRIK*LSINVVELFKFLNWKKILMKP
*YQLFIKLLIVVQYCIHYKSVCGWMNNINVAFS
QMNIPLILFFMIIINSLSLGCK*KCEYIPCCTVG
KILS*CIQNG*HN**EEFGRNWYFNTVCIYYCY
AGCASG*QWPAF*TTLTPRGFCSPKYNLRGFLH
SLNLL*KYCVCVHSLQHFL*LTQ*VSLGFSRPPH
LFFLFLETESCFVARLECSGAISAHHNRCLLGS
SNSPASASRVAGTTGVRTCQANFCIFSRDGVSP
CWPGWSRSLDLMIYPPWPPKVLRLQA*ATVPGQA
PSLLMETGSCTITQAGVQWHNHTSLQPQTPGFK
QSSCLSLPSS*DYRHEPPHPANF*VFL*RQGLT
MLSRLVLNSWPQVILLPQPPKVLGLQI*ATGLAF
SSSFCEVKLVCWKE*ILLVYPFLTVAAGSPVPY
LDSSCQYLSWTLFLLPLVSYISDFLLE*NLIFP
NPHFFLF*KAVSPLLNVSH*GGANFNHKP**DFS
KKCEIEQFSSNSIYF*MNGIVNAILLMNFKRIL
WFSV*FQMSHCNSRRLTFQPTYFPFPCLSHPLF
LPSFLSLLLSPRLIRKNSF*QGKKLNN*LFLTS*
SGTLVEA*I*RNIFSEIYY*GQWR*INSRCGSQ
KI*SKFKDFFCFCNWN*IK*LLVLIVDNLFLLL
VHISITVG*VGERVRE*ITQMYFR*AI*PLMES*
IQ*IQSFALLRRFFVFKWWVKELVYRLTVI*AN
VKSETLILSKEISVNGMSP*NTQIIILFVHTVR
GSSHVGRV*SITFWN*KPLTVIKYNNTHQVLKSL
EWPLS*KEIRSQVRWHVPVIPAPWEAKTGGFLE
PWSLSPAWVT*QDPVLKEKWEERQGNMKKEEIP
SMMELQISWQFMQSVKRRILFCSLQMSISKAFSL
AVFFCIINYIGLHIYNFFLHYMSLVCFETLVFM
I*LI*FFCLGIHFKI*I*KIFVKIKFTKLF*K

Frame 1

*AVAGVRGGSGGRPLGLGVCLFA*AGARRPGWV
DPAGFDCSAVQRRRRRGSEWT*LLYPGQKG*NQ
RWGVWAVGDPRRLEVPQRHPE*RRAPSGVQPPSG
EGRHRPGRPPPFVSSSGVHRRAARGPGLTAALR
DCGVRLRWVDAHLANLRRSLGVFVRPGAAEIQG
RRL*GPDLPRGEGYVARQSPAPLIPGGKLLLPFP
STGLESPSLVPAVPPSPLRPRRKGLAHG*QLRE
RKI*GKMDAKARNCLLQHREALEKDIKTSYIMD
HMISDGFLTISEEEKVRNEPTQQQRAAMLIKMIL
KKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
SSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQ
KLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHS
LLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTR
LDQDESFSQRLPLNIEEAKDRLRILMLRKHPRS
LLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDS
VMGPKYVVPVESSLGKEKGLEILSLFVNMKKAD
LPEQAHSIIKECKGSPLVVSLIGALLRDFPNRW
EYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISV
EMLREDIKDYYTDLSILQKDVKVPTKVLCILWD
METEEVEDILQEFVNKSLLFCDRNGKSFRYYLH
DLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHT
LSPDQEDCMYWYNFLAYHMASAKMHKELCALMF
SLDWIKAKTELVGPAHLIHEFVEYRHILDEKDC
AVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPE
TSEVYQQAKLQAKQEVDNGMLYLEWINKKNITN
LSRLVVRPHTDAVYHACFSEDGQRIASCGADKT
LQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFI
ATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCC
HFTNSSHHLLLATGSSDCFLKLWDLNQKECRNT
MFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDA
TSANERKSINVKQFFLNLEDPQEDMEVIVKCCS
WSADGARIMVAAKNKIFLFDIHTSGLLGEIHTG
HHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSR
SKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQT
IRLWETKKVCKNSAVMLKQEVDVVFQENEVMVL
AVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPH
LQYIAFGDENGAIEILELVNNRIFQSRFQHKKT
VWHIQFTADEKTLISSSDDAEIQVWNWQLDKCI
FLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNI
ITGNKEKDFVCHQGTVLSCDISHDATKFSSTSA
DKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVD
STLLATGDDNGEIRIWNVSNGELLHLCAPLSEEG
AATHGGWVTDLCFSPDGKMLISAGGYIKWWNVV
TGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNL
GILYILQTLE*NS*ALM*LNFLNF*IGKKF**NP
DINFL*SS*LLCSIAFITKVFVVG*IILM*LFP
K*TYL*SCFS*SSLTVCP*DANENVNTYLVVLL
VKFCLDAFKMVDIINEKNLEEIGILILSVFITVM
QAVPQGSSGLLFEPHLPQGGFVLLNTILEVFCT
L*ICFKNIVSVCIVCSISFN*LNK*VLDLAGPP
TFFFCFWRQSLALLPGWSAVARSRLTTIAASWVQ
AILLPQPPE*LGLQVCAHARLIFVFLVETGFHH
VGRDGLDLLTS*STRLGLPKC*DYRREPPCLAR
PLLF*WRQGLALSPRLECSGIIIPHCSLRLLGSS
NPPASASQVAETTGTSHHTQLIFKFSCRDRVSL
CCLGWS*TLGLK*SSCLSLPKCWDCRYEPLAWP
SAVLFVK*NLYVGKSRFYWSTLFSL*LLAALCHI
WTLVVSI*VGHYSCSLLFLTYQTSYLNET*SFL
ILTFFFFKKQFLHC*MLVIEVGPILIISLNKIF
LRNVK*NNFHLIPFTFR*MAL*MPFF**ISREFS
GFLCNSR*VTVTLED*PSSQPIFLSLVSLILFS
FLLSFLFFYLQG*SGKIAFDRGKNSITSYF*PP
DQEL*LKRKSKETFSLKYIIKGNGDKLIVDVVPR
KYNQNSKIFFVSVTGTKSNDY*C***ITCFYCW
CILV*LWGRSGRG*GNRSLRCILDKLFSL*WNH
KYSEYNPLHC*GGFLFLNGGSRS*FTGLL*FKQM
*KVKP*FYQKKFL*MVCLLRIPKS*FYLYTLLG
AHLM*AEYKVLPFGIKSH*LL*SITTHIRF*KA
LNGPCLKKKLGARCGGTCL*SQLLGRLRQEDSLS
PGV*VQPG*HSKTLS*KKNGKKDKVT*RKKRYL
V*WSCKFHGSSCSRSRGGFCFVVCR*AFLKHFP
LLYFFVL*ITLDFIYIIFFYIICLLYVLKLLYL*
YSLYDFFALVYILKYEFKKFL*K*NSQNCFEK

Frame 2

EPWQECAAAAVAAPWGLGCVYLHKRARAVRAGW
IRRDLTAPLSRGGEEEVASGRDCSIPGKRDRTR
GGESGQSATREDLRCRSGIRSSAGLPPGCSRRR
GKGATGREDLLPLCPVVGSTGGRPVGRASPRRSG
TVGSGCVGWTPTSPTFGGPWGSSCAPGLQRSRG
GACEARTCPGAKGMWRDRALHP*FPVENSCCRF
PPPAWSLPVLSRQCRPPH*DLGAKAWLMVDSSER
ERSEGRWMQKLEIVCFNIEKLWKRTSRHPTSWI
T*LVMDF*QYQKRKK*EMSPLNSKEQLC*LK*Y
LKKIMIPTYHSTMLYYMKDIKILLPFSMMAFLLS
LLPV*GQSCVKVEYHRGQLFLSQGRSW*MQFSR
SSPN*KVNQDGSPYMEWQAVGSLY*LQKLLEII
PF*KVVSQGECIGFQLGNKTNLGF**NCRIFAHG
WIRMRVFPRGFHLILKRLKTVSAF*CFANTQGL
S*SWMMFGTLGC*KLLTVSVRFFLQPETRVLQI
Q*WVLNM*SLWRVP*ERKKDLKFYPFLLI*RRQI
CQNKLIVL*KNVKALPL*YL*LVHFYVIFPIAG
STTSNSFRISSLRE*GNLRLMIMRL*MKPCL*V
LKCSEKTSKIITQIFPSFRRTLRCLQRCYVFSGT
WKLKKLKTYCRSL*ISLFYSVIGMESRFVIIYM
IFK*IFLQRRIAASFRIYIRR*SLSFRDITSRI
LFHQIRKTVCIGTTFWPITWPVPRCTRNFVL*CF
PWIGLKQKQNL*ALLI*FMNLWNTDIY*MKRIV
QSVRIFRSFYL*MDTFLDDSHFLILYNWVSVSR
KLQKFISKLSCRPSRRSIMECFTWNG*TKKTSRI
FPA*LSAPTQMLFTMPAFLRMVRE*LLVELIKP
YRCSKLKQERNF*KSRLMRMKCFVVHSLQMTDL
*QPAQWIKK*RFGIL*LGN*YTPMMSTQSKSIAA
ISPTVVIIFS*PLGQVTASSNFGI*IKKNVEIP
CLVIQIQSITADFHQMISFWLVVQLMEP*SFGM
RHQQMRGKALM*NSSS*IWRTLKRIWK***SVVR
GLLMVQG*WWQQKIKSFFLTFILVAYWEKSTRA
ITAPSSTVTSPHKTIWQWLLCPSTV*SCGIQTH
VQRWLIAEDI*VGFMV*CFLLMDHHF*HLLMTRQ
SGSGRQRKYVRTLL*C*SKK*MLCFKKMK*WSL
QLTI*DVCNSLMEEQVRLII*LKLKLAAVA*VH
IFSTLHLEMKMEPLRF*NL*TIESSSPGFSTRKL
YGTSSSQPMRRLLFQVLMMLKFRYGIGNWTNVS
FYEAIRKQ*KTLDS*KIQDCFLGHLMEQ*RYGI
LLLEIKKKTLSVTRVQYFLVTFLTMLPSFHLPLL
TRLQRSGVLISFCHFMN*GATTAVCAALPSLWT
VPCWQREMTMEKSGYGMSQTVSFFICVLRFQKK
ELLPMEAG*LTFAFLQMAKCLSLLEDILSGGTLS
LGNPHRPSTQMEPILRKYTCPLTSKHM*LWIIL
VFYIFYRL*NKIVKH*CS*TF*IFELEKNSNET
LISTFYKALNCCAVLHSLQKCLWLDE*Y*CSFFP
NEHTFNLVFHDHH*QFVLRMQMKM*IHTLLYCW
*NSVLMHSKWLT*LMRRIWKKLVF*YCLYLLLL
CRLCLRVAVACFLNHTYPKGVLFS*IQS*RFFAL
FKFALKILCLCA*SAAFPLIDSISESWI*QAPP
PFFFVFGDRVLLCCQAGVQWRDLGSPQSLPPGF
KQFSCLSLPSSWDYRCAHMPG*FLYF**RRGFTM
LAGMVSIS*PHDLPALASQSAEITGVSHRAWPG
PFSFNGDRVLHYHPGWSAVA*SYLIAASDSWVQ
AILLPQPPK*LRLQARATTPS*FLSFLVETGSHY
VV*AGLELLASSNPPASASQSVGIADMSHWPGL
QQFFL*SKTCMLERVDFIGLPFSHCSCWQPCAI
SGL*LSVSELDTIPAPSCFLHIRLLT*MKPDLS*
SSLFSFLKSSFSTAKC*SLRWGQF*S*ALIRFF
*EM*NRTIFI*FHLLLDEWHCECHSFNEFQENS
LVFCVIPDESL*L*KINLPANLFSFPLSLSSSFP
SFFPFSSFISKVNQEK*LLTGEKTQ*LAIFDLL
IRNFS*SVNLKKHFL*NILLRAMEIN***MWFP
ENIIKIQRFFLFL*LELNQMITSVNSR*LVFIVG
AY*YNCGVGRGEGKGIDHSDVF*ISYLAFDGII
NTVNTILCIVKEVFCF*MVGQGASLQAYCDLSK
CEK*NLNFIKRNFCKWYVSLEYPNHNFICTHC*G
LISCRQSIKYYLLELKATDCYKV*QHTSGFKKP
*MALVLKRN*EPGAVARACNPSSLGG*DRRIP*
ALEFESSLGDIARPCLKRKMGRKTR*HEERRDT*
YDGAANFMAVHAVGQEEDFVL*FADEHF*SIFP
CCIFLYYKLHWTSYI*FFFTLYVSCMF*NSCIY
DIAYMIFLPWYTF*NMNLKNFCKNKIHKIVLKN

Sequence APAF1.aAug05

ATGAGCCGTGGCAGGAGTGCGCGGCGGCAGCGGTGGCCGCCCCTTGGGGCTTGGGGTGTGTTTATTTGCATAAGCGGGCGCGCGCCGTCCGGGCTGGGTG
GATCCGGCGGGATTTGACTGCTCCGCTGTCCAGAGGCGGAGAAGAAGAGGTAGCGAGTGGACGTGACTGCTCTATCCCGGGCAAAAGGGATAGAACCAGA
GGTGGGGAGTCTGGGCAGTCGGCGACCCGCGAAGACTTGAGGTGCCGCAGCGGCATCCGGAGTAGCGCCGGGCTCCCTCCGGGGTGCAGCCGCCGTCGGG
GGAAGGGCGCCACAGGCCGGGAAGACCTCCTCCCTTTGTGTCCAGTAGTGGGGTCCACCGGAGGGCGGCCCGTGGGCCGGGCCTCACCGCGGCGCTCCGG
GACTGTGGGGTCAGGCTGCGTTGGGTGGACGCCCACCTCGCCAACCTTCGGAGGTCCCTGGGGGTCTTCGTGCGCCCCGGGGCTGCAGAGATCCAGGGGA
GGCGCCTGTGAGGCCCGGACCTGCCCCGGGGCGAAGGGTATGTGGCGAGACAGAGCCCTGCACCCCTAATTCCCGGTGGAAAACTCCTGTTGCCGTTTCC
CTCCACCGGCCTGGAGTCTCCCAGTCTTGTCCCGGCAGTGCCGCCCTCCCCACTAAGACCTAGGCGCAAAGGCTTGGCTCATGGTTGACAGCTCAGAGAG
AGAAAGATCTGAGGGAAGATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACATCATGGATC
ACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACT
TAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TCTTCTTCCAGTGGTAAAGATTCAGTTAGTGGAATAACTTCGTATGTAAGGACAGTCCTGTGTGAAGGTGGAGTACCACAGAGGCCAGTTGTTTTTGTCA
CAAGGAAGAAGCTGGTGAATGCAATTCAGCAGAAGCTCTCCAAATTGAAAGGTGAACCAGGATGGGTCACCATACATGGAATGGCAGGCTGTGGGAAGTC
TGTATTAGCTGCAGAAGCTGTTAGAGATCATTCCCTTTTAGAAGGTTGTTTCCCAGGGGGAGTGCATTGGGTTTCAGTTGGGAAACAAGACAAATCTGGG
CTTCTGATGAAACTGCAGAATCTTTGCACACGGTTGGATCAGGATGAGAGTTTTTCCCAGAGGCTTCCACTTAATATTGAAGAGGCTAAAGACCGTCTCC
GCATTCTGATGCTTCGCAAACACCCAAGGTCTCTCTTGATCTTGGATGATGTTTGGGACTCTTGGGTGTTGAAAGCTTTTGACAGTCAGTGTCAGATTCT
TCTTACAACCAGAGACAAGAGTGTTACAGATTCAGTAATGGGTCCTAAATATGTAGTCCCTGTGGAGAGTTCCTTAGGAAAGGAAAAAGGACTTGAAATT
TTATCCCTTTTTGTTAATATGAAGAAGGCAGATTTGCCAGAACAAGCTCATAGTATTATAAAAGAATGTAAAGGCTCTCCCCTTGTAGTATCTTTAATTG
GTGCACTTTTACGTGATTTTCCCAATCGCTGGGAGTACTACCTCAAACAGCTTCAGAATAAGCAGTTTAAGAGAATAAGGAAATCTTCGTCTTATGATTA
TGAGGCTCTAGATGAAGCCATGTCTATAAGTGTTGAAATGCTCAGAGAAGACATCAAAGATTATTACACAGATCTTTCCATCCTTCAGAAGGACGTTAAG
GTGCCTACAAAGGTGTTATGTATTCTCTGGGACATGGAAACTGAAGAAGTTGAAGACATACTGCAGGAGTTTGTAAATAAGTCTCTTTTATTCTGTGATC
GGAATGGAAAGTCGTTTCGTTATTATTTACATGATCTTCAAGTAGATTTTCTTACAGAGAAGAATTGCAGCCAGCTTCAGGATCTACATAAGAAGATAAT
CACTCAGTTTCAGAGATATCACCAGCCGCATACTCTTTCACCAGATCAGGAAGACTGTATGTATTGGTACAACTTTCTGGCCTATCACATGGCCAGTGCC
AAGATGCACAAGGAACTTTGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAACAGAACTTGTAGGCCCTGCTCATCTGATTCATGAATTTGTGG
AATACAGACATATACTAGATGAAAAGGATTGTGCAGTCAGTGAGAATTTTCAGGAGTTTTTATCTTTAAATGGACACCTTCTTGGACGACAGCCATTTCC
TAATATTGTACAACTGGGTCTCTGTGAGCCGGAAACTTCAGAAGTTTATCAGCAAGCTAAGCTGCAGGCCAAGCAGGAGGTCGATAATGGAATGCTTTAC
CTGGAATGGATAAACAAAAAAAACATCACGAATCTTTCCCGCTTAGTTGTCCGCCCCCACACAGATGCTGTTTACCATGCCTGCTTTTCTGAGGATGGTC
AGAGAATAGCTTCTTGTGGAGCTGATAAAACCTTACAGGTGTTCAAAGCTGAAACAGGAGAGAAACTTCTAGAAATCAAGGCTCATGAGGATGAAGTGCT
TTGTTGTGCATTCTCTACAGATGACAGATTTATAGCAACCTGCTCAGTGGATAAAAAAGTGAAGATTTGGAATTCTATGACTGGGGAACTAGTACACACC
TATGATGAGCACTCAGAGCAAGTCAATTGCTGCCATTTCACCAACAGTAGTCATCATCTTCTCTTAGCCACTGGGTCAAGTGACTGCTTCCTCAAACTTT
GGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCATACAAATTCAGTCAATCACTGCAGATTTTCACCAGATGATAAGCTTTTGGCTAGTTG
TTCAGCTGATGGAACCTTAAAGCTTTGGGATGCGACATCAGCAAATGAGAGGAAAAGCATTAATGTGAAACAGTTCTTCCTAAATTTGGAGGACCCTCAA
GAGGATATGGAAGTGATAGTGAAGTGTTGTTCGTGGTCTGCTGATGGTGCAAGGATAATGGTGGCAGCAAAAAATAAAATCTTTCTTTTTGACATTCATA
CTAGTGGCCTATTGGGAGAAATCCACACGGGCCATCACAGCACCATCCAGTACTGTGACTTCTCCCCACAAAACCATTTGGCAGTGGTTGCTTTGTCCCA
GTACTGTGTAGAGTTGTGGAATACAGACTCACGTTCAAAGGTGGCTGATTGCAGAGGACATTTAAGTTGGGTTCATGGTGTGATGTTTTCTCCTGATGGA
TCATCATTTTTGACATCTTCTGATGACCAGACAATCAGGCTCTGGGAGACAAAGAAAGTATGTAAGAACTCTGCTGTAATGTTAAAGCAAGAAGTAGATG
TTGTGTTTCAAGAAAATGAAGTGATGGTCCTTGCAGTTGACCATATAAGACGTCTGCAACTCATTAATGGAAGAACAGGTCAGATTGATTATCTGACTGA
AGCTCAAGTTAGCTGCTGTTGCTTAAGTCCACATCTTCAGTACATTGCATTTGGAGATGAAAATGGAGCCATTGAGATTTTAGAACTTGTAAACAATAGA
ATCTTCCAGTCCAGGTTTCAGCACAAGAAAACTGTATGGCACATCCAGTTCACAGCCGATGAGAAGACTCTTATTTCAAGTTCTGATGATGCTGAAATTC
AGGTATGGAATTGGCAATTGGACAAATGTATCTTTCTACGAGGCCATCAGGAAACAGTGAAAGACTTTAGACTCTTGAAAAATTCAAGACTGCTTTCTTG
GTCATTTGATGGAACAGTGAAGGTATGGAATATTATTACTGGAAATAAAGAAAAAGACTTTGTCTGTCACCAGGGTACAGTACTTTCTTGTGACATTTCT
CACGATGCTACCAAGTTTTCATCTACCTCTGCTGACAAGACTGCAAAGATCTGGAGTTTTGATCTCCTTTTGCCACTTCATGAATTGAGGGGCCACAACG
GCTGTGTGCGCTGCTCTGCCTTCTCTGTGGACAGTACCCTGCTGGCAACGGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAAACGGTGAGCT
TCTTCATTTGTGTGCTCCGCTTTCAGAAGAAGGAGCTGCTACCCATGGAGGCTGGGTGACTGACCTTTGCTTTTCTCCAGATGGCAAAATGCTTATCTCT
GCTGGAGGATATATTAAGTGGTGGAACGTTGTCACTGGGGAATCCTCACAGACCTTCTACACAAATGGAACCAATCTTAAGAAAATACACGTGTCCCCTG
ACTTCAAAACATATGTGACTGTGGATAATCTTGGTATTTTATATATTTTACAGACTTTAGAATAAAATAGTTAAGCATTAATGTAGTTGAACTTTTTAAA
TTTTTGAATTGGAAAAAAATTCTAATGAAACCCTGATATCAACTTTTTATAAAGCTCTTAATTGTTGTGCAGTATTGCATTCATTACAAAAGTGTTTGTG
GTTGGATGAATAATATTAATGTAGCTTTTTCCCAAATGAACATACCTTTAATCTTGTTTTTCATGATCATCATTAACAGTTTGTCCTTAGGATGCAAATG
AAAATGTGAATACATACCTTGTTGTACTGTTGGTAAAATTCTGTCTTGATGCATTCAAAATGGTTGACATAATTAATGAGAAGAATTTGGAAGAAATTGG
TATTTTAATACTGTCTGTATTTATTACTGTTATGCAGGCTGTGCCTCAGGGTAGCAGTGGCCTGCTTTTTGAACCACACTTACCCCAAGGGGGTTTTGTT
CTCCTAAATACAATCTTAGAGGTTTTTTGCACTCTTTAAATTTGCTTTAAAAATATTGTGTCTGTGTGCATAGTCTGCAGCATTTCCTTTAATTGACTCA
ATAAGTGAGTCTTGGATTTAGCAGGCCCCCCCACCTTTTTTTTTTGTTTTTGGAGACAGAGTCTTGCTTTGTTGCCAGGCTGGAGTGCAGTGGCGCGATC
TCGGCTCACCACAATCGCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGTGTGCGCACATGCCAGGCTAATT
TTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCGGGATGGTCTCGATCTCTTGACCTCATGATCTACCCGCCTTGGCCTCCCAAAGTGCTGAG
ATTACAGGCGTGAGCCACCGTGCCTGGCCAGGCCCCTTCTCTTTTAATGGAGACAGGGTCTTGCACTATCACCCAGGCTGGAGTGCAGTGGCATAATCAT
ACCTCATTGCAGCCTCAGACTCCTGGGTTCAAGCAATCCTCCTGCCTCAGCCTCCCAAGTAGCTGAGACTACAGGCACGAGCCACCACACCCAGCTAATT
TTTAAGTTTTCTTGTAGAGACAGGGTCTCACTATGTTGTCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTAATCCTCCTGCCTCAGCCTCCCAAAGTGTT
GGGATTGCAGATATGAGCCACTGGCCTGGCCTTCAGCAGTTCTTTTTGTGAAGTAAAACTTGTATGTTGGAAAGAGTAGATTTTATTGGTCTACCCTTTT
CTCACTGTAGCTGCTGGCAGCCCTGTGCCATATCTGGACTCTAGTTGTCAGTATCTGAGTTGGACACTATTCCTGCTCCCTCTTGTTTCTTACATATCAG
ACTTCTTACTTGAATGAAACCTGATCTTTCCTAATCCTCACTTTTTTCTTTTTTAAAAAGCAGTTTCTCCACTGCTAAATGTTAGTCATTGAGGTGGGGC
CAATTTTAATCATAAGCCTTAATAAGATTTTTCTAAGAAATGTGAAATAGAACAATTTTCATCTAATTCCATTTACTTTTAGATGAATGGCATTGTGAAT
GCCATTCTTTTAATGAATTTCAAGAGAATTCTCTGGTTTTCTGTGTAATTCCAGATGAGTCACTGTAACTCTAGAAGATTAACCTTCCAGCCAACCTATT
TTCCTTTCCCTTGTCTCTCTCATCCTCTTTTCCTTCCTTCTTTCCTTTCTCTTCTTTTATCTCCAAGGTTAATCAGGAAAAATAGCTTTTGACAGGGGAA
AAAACTCAATAACTAGCTATTTTTGACCTCCTGATCAGGAACTTTAGTTGAAGCGTAAATCTAAAGAAACATTTTCTCTGAAATATATTATTAAGGGCAA
TGGAGATAAATTAATAGTAGATGTGGTTCCCAGAAAATATAATCAAAATTCAAAGATTTTTTTTGTTTCTGTAACTGGAACTAAATCAAATGATTACTAG
TGTTAATAGTAGATAACTTGTTTTTATTGTTGGTGCATATTAGTATAACTGTGGGGTAGGTCGGGGAGAGGGTAAGGGAATAGATCACTCAGATGTATTT
TAGATAAGCTATTTAGCCTTTGATGGAATCATAAATACAGTGAATACAATCCTTTGCATTGTTAAGGAGGTTTTTTGTTTTTAAATGGTGGGTCAAGGAG
CTAGTTTACAGGCTTACTGTGATTTAAGCAAATGTGAAAAGTGAAACCTTAATTTTATCAAAAGAAATTTCTGTAAATGGTATGTCTCCTTAGAATACCC
AAATCATAATTTTATTTGTACACACTGTTAGGGGCTCATCTCATGTAGGCAGAGTATAAAGTATTACCTTTTGGAATTAAAAGCCACTGACTGTTATAAA
GTATAACAACACACATCAGGTTTTAAAAAGCCTTGAATGGCCCTTGTCTTAAAAAGAAATTAGGAGCCAGGTGCGGTGGCACGTGCCTGTAATCCCAGCT
CCTTGGGAGGCTAAGACAGGAGGATTCCTTGAGCCCTGGAGTTTGAGTCCAGCCTGGGTGACATAGCAAGACCCTGTCTTAAAAGAAAAATGGGAAGAAA
GACAAGGTAACATGAAGAAAGAAGAGATACCTAGTATGATGGAGCTGCAAATTTCATGGCAGTTCATGCAGTCGGTCAAGAGGAGGATTTTGTTTTGTAG
TTTGCAGATGAGCATTTCTAAAGCATTTTCCCTTGCTGTATTTTTTTGTATTATAAATTACATTGGACTTCATATATATAATTTTTTTTTACATTATATG
TCTCTTGTATGTTTTGAAACTCTTGTATTTATGATATAGCTTATATGATTTTTTTGCCTTGGTATACATTTTAAAATATGAATTTAAAAAATTTTTGTAA
AAATAAAATTCACAAAATTGTTTTGAAAAAC
  • APAF1-3 (forward primer)
  • APAF1-4 (reverse primer)
  • Single exon skipping

Frame 0

MSRGRSARRQRWPPLGAWGVFICISGRAPSGLG
GSGGI*LLRCPEAEKKR*RVDVTALSRAKGIEPE
VGSLGSRRPAKT*GAAAASGVAPGSLRGAAAVG
GRAPQAGKTSSLCVQ*WGPPEGGPWAGPHRGAP
GLWGQAALGGRPPRQPSEVPGGLRAPRGCRDPGE
APVRPGPAPGRRVCGETEPCTPNSRWKTPVAVS
LHRPGVSQSCPGSAALPTKT*AQRLGSWLTAQR
EKDLREDGCKSSKLFAST*RSSGKGHQDILHHGS
HD**WIFNNIRRGKSKK*AHSTAKSSYAD*NDT
*KR**FLRIILQCSTT*RI*RSCCPSP*WHSCC
LFFQW*RFS*WNNFVCKDSPV*RWSTTEASCFCH
KEEAGECNSAEALQIER*TRMGHHTWNGRLWEV
CISCRSC*RSFPFRRLFPRGSALGFSWETRQIW
ASDETAESLHTVGSG*EFFPEAST*Y*RG*RPSP
HSDASQTPKVSLDLG*CLGLLGVESF*QSVSDS
SYNQRQECYRFSNGS*ICSPCGEFLRKGKRT*N
FIPFC*YEEGRFARTSS*YYKRM*RLSPCSIFNW
CTFT*FSQSLGVLPQTASE*AV*ENKEIFVL*L
*GSR*SHVYKC*NAQRRHQRLLHRSFHPSEGR*
GAYKGVMYSLGHGN*RS*RHTAGVCK*VSFIL*S
EWKVVSLLFT*SSSRFSYREELQPASGST*EDN
HSVSEISPAAYSFTRSGRLYVLVQLSGLSHGQC
QDAQGTLCFNVFPGLD*SKNRTCRPCSSDS*ICG
IQTYTR*KGLCSQ*EFSGVFIFKWTPSWTTAIS
*YCTTGSL*AGNFRSLSAS*AAGQAGGR*WNAL
PGMDKQKKHHESFPLSCPPPHRCCLPCLLF*GWS
ENSFLWS**NLTGVQS*NRRETSRNQGS*G*SA
LLCILYR*QIYSNLLSG*KSEDLEFYDWGTSTH
L**ALRASQLLPFHQQ*SSSSLSHWVK*LLPQTL
GFESKRMSKYHVWSYKFSQSLQIFTR**AFG*L
FS*WNLKALGCDISK*EEKH*CETVLPKFGGPS
RGYGSDSEVLFVVC*WCKDNGGSKK*NLSF*HSY
*WPIGRNPHGPSQHHPVL*LLPTKPFGSGCFVP
VLCRVVEYRLTFKGG*LQRTFKLGSWCDVFS*W
IIIFDIF**PDNQALGDKESM*ELCCNVKARSRC
CVSRK*SDGPCS*PYKTSATH*WKNRSD*LSD*
SSS*LLLLKSTSSVHCIWR*KWSH*DFRTCKQ*
NLPVQVSAQENCMAHPVHSR*EDSYFKF**C*NS
GMELAIGQMYLSTRPSGNSERL*TLEKFKTAFL
VI*WNSEGMEYYYWK*RKRLCLSPGYSTFL*HF
SRCYQVFIYLC*QDCKDLEF*SPFATS*IEGPQR
LCALLCLLCGQYPAGNGR*QWRNQDMECLKR*A
SSFVCSAFRRRSCYPWRLGD*PLLFSRWQNAYL
CWRIY*VVERCHWGILTDLLHKWNQS*ENTRVP*
LQNICDCG*SWYFIYFTDFRIK*LSINVVELFK
FLNWKKILMKP*YQLFIKLLIVVQYCIHYKSVC
GWMNNINVAFSQMNIPLILFFMIIINSLSLGCK*
KCEYIPCCTVGKILS*CIQNG*HN**EEFGRNW
YFNTVCIYYCYAGCASG*QWPAF*TTLTPRGFC
SPKYNLRGFLHSLNLL*KYCVCVHSLQHFL*LTQ
*VSLGFSRPPHLFFLFLETESCFVARLECSGAI
SAHHNRCLLGSSNSPASASRVAGTTGVRTCQAN
FCIFSRDGVSPCWPGWSRSLDLMIYPPWPPKVLR
LQA*ATVPGQAPSLLMETGSCTITQAGVQWHNH
TSLQPQTPGFKQSSCLSLPSS*DYRHEPPHPAN
F*VFL*RQGLTMLSRLVLNSWPQVILLPQPPKVL
GLQI*ATGLAFSSSFCEVKLVCWKE*ILLVYPF
LTVAAGSPVPYLDSSCQYLSWTLFLLPLVSYIS
DFLLE*NLIFPNPHFFLF*KAVSPLLNVSH*GGA
NFNHKP**DFSKKCEIEQFSSNSIYF*MNGIVN
AILLMNFKRILWFSV*FQMSHCNSRRLTFQPTY
FPFPCLSHPLFLPSFLSLLLSPRLIRKNSF*QGK
KLNN*LFLTS*SGTLVEA*I*RNIFSEIYY*GQ
WR*INSRCGSQKI*SKFKDFFCFCNWN*IK*LL
VLIVDNLFLLLVHISITVG*VGERVRE*ITQMYF
R*AI*PLMES*IQ*IQSFALLRRFFVFKWWVKE
LVYRLTVI*ANVKSETLILSKEISVNGMSP*NT
QIIILFVHTVRGSSHVGRV*SITFWN*KPLTVIK
YNNTHQVLKSLEWPLS*KEIRSQVRWHVPVIPA
PWEAKTGGFLEPWSLSPAWVT*QDPVLKEKWEE
RQGNMKKEEIPSMMELQISWQFMQSVKRRILFCS
LQMSISKAFSLAVFFCIINYIGLHIYNFFLHYM
SLVCFETLVFMI*LI*FFCLGIHFKI*I*KIFV
KIKFTKLF*K

Frame 1

*AVAGVRGGSGGRPLGLGVCLFA*AGARRPGWV
DPAGFDCSAVQRRRRRGSEWT*LLYPGQKG*NQ
RWGVWAVGDPRRLEVPQRHPE*RRAPSGVQPPSG
EGRHRPGRPPPFVSSSGVHRRAARGPGLTAALR
DCGVRLRWVDAHLANLRRSLGVFVRPGAAEIQG
RRL*GPDLPRGEGYVARQSPAPLIPGGKLLLPFP
STGLESPSLVPAVPPSPLRPRRKGLAHG*QLRE
RKI*GKMDAKARNCLLQHREALEKDIKTSYIMD
HMISDGFLTISEEEKVRNEPTQQQRAAMLIKMIL
KKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
SSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFV
TRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKS
VLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSG
LLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRL
RILMLRKHPRSLLILDDVWDSWVLKAFDSQCQIL
LTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEI
LSLFVNMKKADLPEQAHSIIKECKGSPLVVSLI
GALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDY
EALDEAMSISVEMLREDIKDYYTDLSILQKDVK
VPTKVLCILWDMETEEVEDILQEFVNKSLLFCD
RNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKII
TQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASA
KMHKELCALMFSLDWIKAKTELVGPAHLIHEFV
EYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFP
NIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLY
LEWINKKNITNLSRLVVRPHTDAVYHACFSEDG
QRIASCGADKTLQVFKAETGEKLLEIKAHEDEVL
CCAFSTDDRFIATCSVDKKVKIWNSMTGELVHT
YDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKL
WDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASC
SADGTLKLWDATSANERKSINVKQFFLNLEDPQ
EDMEVIVKCCSWSADGARIMVAAKNKIFLFDIH
TSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQ
YCVELWNTDSRSKVADCRGHLSWVHGVMFSPDG
SSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVD
VVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTE
AQVSCCCLSPHLQYIAFGDENGAIEILELVNNR
IFQSRFQHKKTVWHIQFTADEKTLISSSDDAEI
QVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSW
SFDGTVKVWNIITGNKEKDFVCHQGTVLSCDIS
HDATKFSSTSADKTAKIWSFDLLLPLHELRGHN
GCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGEL
LHLCAPLSEEGAATHGGWVTDLCFSPDGKMLIS
AGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSP
DFKTYVTVDNLGILYILQTLE*NS*ALM*LNFLN
F*IGKKF**NPDINFL*SS*LLCSIAFITKVFV
VG*IILM*LFPK*TYL*SCFS*SSLTVCP*DAN
ENVNTYLVVLLVKFCLDAFKMVDIINEKNLEEIG
ILILSVFITVMQAVPQGSSGLLFEPHLPQGGFV
LLNTILEVFCTL*ICFKNIVSVCIVCSISFN*L
NK*VLDLAGPPTFFFCFWRQSLALLPGWSAVARS
RLTTIAASWVQAILLPQPPE*LGLQVCAHARLI
FVFLVETGFHHVGRDGLDLLTS*STRLGLPKC*
DYRREPPCLARPLLF*WRQGLALSPRLECSGIII
PHCSLRLLGSSNPPASASQVAETTGTSHHTQLI
FKFSCRDRVSLCCLGWS*TLGLK*SSCLSLPKC
WDCRYEPLAWPSAVLFVK*NLYVGKSRFYWSTLF
SL*LLAALCHIWTLVVSI*VGHYSCSLLFLTYQ
TSYLNET*SFLILTFFFFKKQFLHC*MLVIEVG
PILIISLNKIFLRNVK*NNFHLIPFTFR*MAL*M
PFF**ISREFSGFLCNSR*VTVTLED*PSSQPI
FLSLVSLILFSFLLSFLFFYLQG*SGKIAFDRG
KNSITSYF*PPDQEL*LKRKSKETFSLKYIIKGN
GDKLIVDVVPRKYNQNSKIFFVSVTGTKSNDY*
C***ITCFYCWCILV*LWGRSGRG*GNRSLRCI
LDKLFSL*WNHKYSEYNPLHC*GGFLFLNGGSRS
*FTGLL*FKQM*KVKP*FYQKKFL*MVCLLRIP
KS*FYLYTLLGAHLM*AEYKVLPFGIKSH*LL*
SITTHIRF*KALNGPCLKKKLGARCGGTCL*SQL
LGRLRQEDSLSPGV*VQPG*HSKTLS*KKNGKK
DKVT*RKKRYLV*WSCKFHGSSCSRSRGGFCFV
VCR*AFLKHFPLLYFFVL*ITLDFIYIIFFYIIC
LLYVLKLLYL*YSLYDFFALVYILKYEFKKFL*
K*NSQNCFEK

Frame 2

EPWQECAAAAVAAPWGLGCVYLHKRARAVRAGW
IRRDLTAPLSRGGEEEVASGRDCSIPGKRDRTR
GGESGQSATREDLRCRSGIRSSAGLPPGCSRRR
GKGATGREDLLPLCPVVGSTGGRPVGRASPRRSG
TVGSGCVGWTPTSPTFGGPWGSSCAPGLQRSRG
GACEARTCPGAKGMWRDRALHP*FPVENSCCRF
PPPAWSLPVLSRQCRPPH*DLGAKAWLMVDSSER
ERSEGRWMQKLEIVCFNIEKLWKRTSRHPTSWI
T*LVMDF*QYQKRKK*EMSPLNSKEQLC*LK*Y
LKKIMIPTYHSTMLYYMKDIKILLPFSMMAFLLS
LLPVVKIQLVE*LRM*GQSCVKVEYHRGQLFLS
QGRSW*MQFSRSSPN*KVNQDGSPYMEWQAVGS
LY*LQKLLEIIPF*KVVSQGECIGFQLGNKTNLG
F**NCRIFAHGWIRMRVFPRGFHLILKRLKTVS
AF*CFANTQGLS*SWMMFGTLGC*KLLTVSVRF
FLQPETRVLQIQ*WVLNM*SLWRVP*ERKKDLKF
YPFLLI*RRQICQNKLIVL*KNVKALPL*YL*L
VHFYVIFPIAGSTTSNSFRISSLRE*GNLRLMI
MRL*MKPCL*VLKCSEKTSKIITQIFPSFRRTLR
CLQRCYVFSGTWKLKKLKTYCRSL*ISLFYSVI
GMESRFVIIYMIFK*IFLQRRIAASFRIYIRR*
SLSFRDITSRILFHQIRKTVCIGTTFWPITWPVP
RCTRNFVL*CFPWIGLKQKQNL*ALLI*FMNLW
NTDIY*MKRIVQSVRIFRSFYL*MDTFLDDSHF
LILYNWVSVSRKLQKFISKLSCRPSRRSIMECFT
WNG*TKKTSRIFPA*LSAPTQMLFTMPAFLRMV
RE*LLVELIKPYRCSKLKQERNF*KSRLMRMKC
FVVHSLQMTDL*QPAQWIKK*RFGIL*LGN*YTP
MMSTQSKSIAAISPTVVIIFS*PLGQVTASSNF
GI*IKKNVEIPCLVIQIQSITADFHQMISFWLV
VQLMEP*SFGMRHQQMRGKALM*NSSS*IWRTLK
RIWK***SVVRGLLMVQG*WWQQKIKSFFLTFI
LVAYWEKSTRAITAPSSTVTSPHKTIWQWLLCP
STV*SCGIQTHVQRWLIAEDI*VGFMV*CFLLMD
HHF*HLLMTRQSGSGRQRKYVRTLL*C*SKK*M
LCFKKMK*WSLQLTI*DVCNSLMEEQVRLII*L
KLKLAAVA*VHIFSTLHLEMKMEPLRF*NL*TIE
SSSPGFSTRKLYGTSSSQPMRRLLFQVLMMLKF
RYGIGNWTNVSFYEAIRKQ*KTLDS*KIQDCFL
GHLMEQ*RYGILLLEIKKKTLSVTRVQYFLVTFL
TMLPSFHLPLLTRLQRSGVLISFCHFMN*GATT
AVCAALPSLWTVPCWQREMTMEKSGYGMSQTVS
FFICVLRFQKKELLPMEAG*LTFAFLQMAKCLSL
LEDILSGGTLSLGNPHRPSTQMEPILRKYTCPL
TSKHM*LWIILVFYIFYRL*NKIVKH*CS*TF*
IFELEKNSNETLISTFYKALNCCAVLHSLQKCLW
LDE*Y*CSFFPNEHTFNLVFHDHH*QFVLRMQM
KM*IHTLLYCW*NSVLMHSKWLT*LMRRIWKKL
VF*YCLYLLLLCRLCLRVAVACFLNHTYPKGVLF
S*IQS*RFFALFKFALKILCLCA*SAAFPLIDS
ISESWI*QAPPPFFFVFGDRVLLCCQAGVQWRD
LGSPQSLPPGFKQFSCLSLPSSWDYRCAHMPG*F
LYF**RRGFTMLAGMVSIS*PHDLPALASQSAE
ITGVSHRAWPGPFSFNGDRVLHYHPGWSAVA*S
YLIAASDSWVQAILLPQPPK*LRLQARATTPS*F
LSFLVETGSHYVV*AGLELLASSNPPASASQSV
GIADMSHWPGLQQFFL*SKTCMLERVDFIGLPF
SHCSCWQPCAISGL*LSVSELDTIPAPSCFLHIR
LLT*MKPDLS*SSLFSFLKSSFSTAKC*SLRWG
QF*S*ALIRFF*EM*NRTIFI*FHLLLDEWHCE
CHSFNEFQENSLVFCVIPDESL*L*KINLPANLF
SFPLSLSSSFPSFFPFSSFISKVNQEK*LLTGE
KTQ*LAIFDLLIRNFS*SVNLKKHFL*NILLRA
MEIN***MWFPENIIKIQRFFLFL*LELNQMITS
VNSR*LVFIVGAY*YNCGVGRGEGKGIDHSDVF
*ISYLAFDGIINTVNTILCIVKEVFCF*MVGQG
ASLQAYCDLSKCEK*NLNFIKRNFCKWYVSLEYP
NHNFICTHC*GLISCRQSIKYYLLELKATDCYK
V*QHTSGFKKP*MALVLKRN*EPGAVARACNPS
SLGG*DRRIP*ALEFESSLGDIARPCLKRKMGRK
TR*HEERRDT*YDGAANFMAVHAVGQEEDFVL*
FADEHF*SIFPCCIFLYYKLHWTSYI*FFFTLY
VSCMF*NSCIYDIAYMIFLPWYTF*NMNLKNFCK
NKIHKIVLKN

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