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Sequence SPP1.kAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCACTAAAGATGTACCTACCCCTCCACAACAGATGAAACTGTG
CCAGCCAAACAACAAATGGGCATTGTCCCCAGAAGCTTGGACAAAAAGGCACACAGAGTTCAATTCCAGTTGAACAGAATAAAGGCCAAAATAGAGCTGC
CTTGGGGGTCACTGCAATTAGACTGCTTAATGAAGACATTAAAAGAAGTATTCTGTCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACCC
TGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGAT
GATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTG
ATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGG
TGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGGTAAATCCTTTAACAGACACACCTGATGGTTCTGACTAG
CGCTCAAGTCTAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTCATCCATTCAGCAAGAATTCATTCATATTCTACTTTATGACCA
TTGAATACAAATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTAGATTTGATTCTCAAACACAATTCTACTTTTTGAAATCCTGGA
TCACTTATTTTCAGATTAAAATAAATGGAAAACACCAATTATTT
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQH*RCTYPSTTDETV
PAKQQMGIVPRSLDKKAHRVQFQLNRIKAKIEL
PWGSLQLDCLMKTLKEVFCLYNKYPDAVATWLNP
DPSQKQNLLAPQTLPSKSNESHDHMDDMDDEDD
DDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHS
DESDELVTDFPTDLPATEVFTPVVPTVDTYDGRG
DSVVYGLRSKSKKFRRPDIQVNPLTDTPDGSD*
RSSLGNHSLHIHSFIHPFIHPFSKNSFIFYFMT
IEYKSFSAWRFL*VYIISL*I*FSNTILLFEILD
HLFSD*NKWKTPII
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSTKDVPTPPQQMKLC
QPNNKWALSPEAWTKRHTEFNSS*TE*RPK*SC
LGGHCN*TA**RH*KKYSVFTTNTQMLWPHG*T
LTHLRSRIS*PHRPFQVSPTKAMTTWMIWMMKMM
MTMWTARTPLTRTTLMM*MTLMILTSLMSLTIL
MNLMNWSLIFPRTCQQPKFSLQLSPQ*THMMAE
VIVWFMD*GQNLRSFADLTSR*IL*QTHLMVLTS
AQV*ETTVCIFIHSFIHSFIHSARIHSYSTL*P
LNTNLFLLGGFCKST*FLSRFDSQTQFYFLKSW
ITYFQIKINGKHQLF
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALKMYLPLHNR*NC
ASQTTNGHCPQKLGQKGTQSSIPVEQNKGQNRAA
LGVTAIRLLNEDIKRSILSLQQIPRCCGHMAKP
*PISEAESPSPTDPSK*VQRKP*PHG*YG**R*
**PCGQPGLH*LERL**CR*H**FSPV**VSPF*
*I**TGH*FSHGPASNRSFHSSCPHSRHI*WPR
**CGLWTEVKI*EVSQT*HPGKSFNRHT*WF*L
ALKSRKPQFAYSFIHSSIHSSIQQEFIHILLYDH
*IQIFFCLAVFVSLHNFSLDLILKHNSTF*NPG
SLIFRLK*MENTNY
Sequence SPP1.hAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTTCCAAGTAAG
TCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATG
TAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGA
AGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCT
GACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACC
TGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAG
ATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAA
TTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTT
CTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTT
CTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGT
AAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTT
ATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTG
TGATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAAT
TAATAAAACATAACCTTTTTTACTGCCTATATAA
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEDPSQKQNLLAPQTLPSK
SNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDD
VDDTDDSHQSDESHHSDESDELVTDFPTDLPATE
VFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRP
DIQYPDATDEDITSHMESEELNGAYKAIPVAQD
LNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSR
LYKRKANDESNEHSDVIDSQELSKVSREFHSHE
FHSHEDMLVVDPKSKEEDKHLKFRISHELDSAS
SEVN*KEKKYNFSLCI*SKEKMLYSKMKENMKCF
FLSLLVECVSI*VWK*LMCLIISLVCGFMETPC
KLKASGLCLCSFYRRNANYHCILIFVILS*IEI
YVEANKILLPT*KENITFYVTIIFCFLS*CIFCC
DYLFVV*INLLS*M**EFGGVNCLFVFPRLSSN
**NITFFTAYI
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRTHLRSRIS*PHRPFQVS
PTKAMTTWMIWMMKMMMTMWTARTPLTRTTLMM
*MTLMILTSLMSLTILMNLMNWSLIFPRTCQQP
KFSLQLSPQ*THMMAEVIVWFMD*GQNLRSFADL
TSSTLMLQTRTSPHTWKARS*MVHTRPSPLPRT
*TRLLIGTAVGRTVMKRVSWMTRVLKPTATSSP
DYISGKPMMRAMSIPM*LIVRNFPKSAVNSTAMN
FTAMKICWL*TPKVRKKINT*NFVFLMN*IVHL
LRSIKRRKNTISHFAFSQKKKCFIAK*KRT*NA
SFSVYWLNVYLFESGNN*CV**LV*FVASWKLPV
N*KLQGYVYVHSIEEMQTITVF*YLLFSHE*KF
M*KQTKYFYPLKKRI*HFMSL*SFVF*VSVYFV
VIIFLWCE*IFYLECNKNLVVSIAYLFSHGCPAI
NKT*PFLLPI*
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GPISEAESPSPTDPSK*
VQRKP*PHG*YG**R***PCGQPGLH*LERL**C
R*H**FSPV**VSPF**I**TGH*FSHGPASNR
SFHSSCPHSRHI*WPR**CGLWTEVKI*EVSQT
*HPVP*CYRRGHHLTHGKRGVEWCIQGHPRCPGP
ERAF*LGQPWEGQL*NESAG*PEC*NPQPQAVQ
II*AESQ**EQ*AFRCD**SGTFQSQP*IPQP*
ISQP*RYAGCRPQK*GRR*TPEISYFS*IR*CIF
*GQLKGEKIQFLTLHLVKRKNAL*QNEREHEML
LSQFIG*MCIYLSLEITNVFDN*FSLWLHGNSL
*TKSFRVMSMFIL*KKCKLSLYFNICYSLMNRNL
CRSKQNTFTHLKREYNILCHYNLLFFKLVYILL
*LSFCGVNKSFILNVIRIWWCQLLICFPTVVQQ
LIKHNLFYCLY
Sequence SPP1.eAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC
CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATG
ATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTC
TGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGA
GGTGATAGTGTGGTTTATGGACTGAGGTCGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACC
TGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAG
ATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAA
TTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTT
CTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTT
CTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGT
AAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTT
ATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTG
TGATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAAT
TAATAAAACATAACCTTTTTTACTGCCTATATAA
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN
PDPSQKQNLLAPQTLPSKSNESHDHMDDMDDED
DDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHS
DESDELVTDFPTDLPATEVFTPVVPTVDTYDGR
GDSVVYGLRSDITSHMESEELNGAYKAIPVAQD
LNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSR
LYKRKANDESNEHSDVIDSQELSKVSREFHSHE
FHSHEDMLVVDPKSKEEDKHLKFRISHELDSAS
SEVN*KEKKYNFSLCI*SKEKMLYSKMKENMKCF
FLSLLVECVSI*VWK*LMCLIISLVCGFMETPC
KLKASGLCLCSFYRRNANYHCILIFVILS*IEI
YVEANKILLPT*KENITFYVTIIFCFLS*CIFCC
DYLFVV*INLLS*M**EFGGVNCLFVFPRLSSN
**NITFFTAYI
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T
LTHLRSRIS*PHRPFQVSPTKAMTTWMIWMMKM
MMTMWTARTPLTRTTLMM*MTLMILTSLMSLTI
LMNLMNWSLIFPRTCQQPKFSLQLSPQ*THMMAE
VIVWFMD*GRTSPHTWKARS*MVHTRPSPLPRT
*TRLLIGTAVGRTVMKRVSWMTRVLKPTATSSP
DYISGKPMMRAMSIPM*LIVRNFPKSAVNSTAMN
FTAMKICWL*TPKVRKKINT*NFVFLMN*IVHL
LRSIKRRKNTISHFAFSQKKKCFIAK*KRT*NA
SFSVYWLNVYLFESGNN*CV**LV*FVASWKLPV
N*KLQGYVYVHSIEEMQTITVF*YLLFSHE*KF
M*KQTKYFYPLKKRI*HFMSL*SFVF*VSVYFV
VIIFLWCE*IFYLECNKNLVVSIAYLFSHGCPAI
NKT*PFLLPI*
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK
P*PISEAESPSPTDPSK*VQRKP*PHG*YG**R*
**PCGQPGLH*LERL**CR*H**FSPV**VSPF
**I**TGH*FSHGPASNRSFHSSCPHSRHI*WP
R**CGLWTEVGHHLTHGKRGVEWCIQGHPRCPGP
ERAF*LGQPWEGQL*NESAG*PEC*NPQPQAVQ
II*AESQ**EQ*AFRCD**SGTFQSQP*IPQP*
ISQP*RYAGCRPQK*GRR*TPEISYFS*IR*CIF
*GQLKGEKIQFLTLHLVKRKNAL*QNEREHEML
LSQFIG*MCIYLSLEITNVFDN*FSLWLHGNSL
*TKSFRVMSMFIL*KKCKLSLYFNICYSLMNRNL
CRSKQNTFTHLKREYNILCHYNLLFFKLVYILL
*LSFCGVNKSFILNVIRIWWCQLLICFPTVVQQ
LIKHNLFYCLY
Sequence SPP1.bAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC
CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATG
ATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTC
TGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGA
GGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACA
TGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGA
AACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGAT
GTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGG
AAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCAT
TTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAA
TAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAA
ATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATAT
AACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAA
TAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAATAAAACATAACCTTTTTTACTGCCTATATAA
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN
PDPSQKQNLLAPQTLPSKSNESHDHMDDMDDED
DDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHS
DESDELVTDFPTDLPATEVFTPVVPTVDTYDGR
GDSVVYGLRSKSKKFRRPDIQYPDATDEDITSH
MESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYE
TSQLDDQSAETHSHKQSRLYKRKANDESNEHSD
VIDSQELSKVSREFHSHEFHSHEDMLVVDPKSK
EEDKHLKFRISHELDSASSEVN*KEKKYNFSLCI
*SKEKMLYSKMKENMKCFFLSLLVECVSI*VWK
*LMCLIISLVCGFMETPCKLKASGLCLCSFYRR
NANYHCILIFVILS*IEIYVEANKILLPT*KENI
TFYVTIIFCFLS*CIFCCDYLFVV*INLLS*M*
*EFGGVNCLFVFPRLSSN**NITFFTAYI
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T
LTHLRSRIS*PHRPFQVSPTKAMTTWMIWMMKM
MMTMWTARTPLTRTTLMM*MTLMILTSLMSLTI
LMNLMNWSLIFPRTCQQPKFSLQLSPQ*THMMAE
VIVWFMD*GQNLRSFADLTSSTLMLQTRTSPHT
WKARS*MVHTRPSPLPRT*TRLLIGTAVGRTVM
KRVSWMTRVLKPTATSSPDYISGKPMMRAMSIPM
*LIVRNFPKSAVNSTAMNFTAMKICWL*TPKVR
KKINT*NFVFLMN*IVHLLRSIKRRKNTISHFA
FSQKKKCFIAK*KRT*NASFSVYWLNVYLFESGN
N*CV**LV*FVASWKLPVN*KLQGYVYVHSIEE
MQTITVF*YLLFSHE*KFM*KQTKYFYPLKKRI
*HFMSL*SFVF*VSVYFVVIIFLWCE*IFYLECN
KNLVVSIAYLFSHGCPAINKT*PFLLPI*
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK
P*PISEAESPSPTDPSK*VQRKP*PHG*YG**R*
**PCGQPGLH*LERL**CR*H**FSPV**VSPF
**I**TGH*FSHGPASNRSFHSSCPHSRHI*WP
R**CGLWTEVKI*EVSQT*HPVP*CYRRGHHLTH
GKRGVEWCIQGHPRCPGPERAF*LGQPWEGQL*
NESAG*PEC*NPQPQAVQII*AESQ**EQ*AFR
CD**SGTFQSQP*IPQP*ISQP*RYAGCRPQK*G
RR*TPEISYFS*IR*CIF*GQLKGEKIQFLTLH
LVKRKNAL*QNEREHEMLLSQFIG*MCIYLSLE
ITNVFDN*FSLWLHGNSL*TKSFRVMSMFIL*KK
CKLSLYFNICYSLMNRNLCRSKQNTFTHLKREY
NILCHYNLLFFKLVYILL*LSFCGVNKSFILNV
IRIWWCQLLICFPTVVQQLIKHNLFYCLY
Sequence SPP1.lAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCT
GAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATG
TGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGA
TGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTG
GTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGGTAAATCCTTTAACAGACACACCTGATGGTTCTGACTAGCGCTCAAGTC
TAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTCATCCATTCAGCAAGAATTCATTCATATTCTACTTTATGACCATTGAATACAA
ATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTAGATTTGATTCTCAAACACAATTCTACTTTTTGAAATCCTGGATCACTTATTT
TCAGATTAAAATAAATGGAAAACACCAATTATTT
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEDPSQKQNLLAPQNAVSS
EETNDFKQETLPSKSNESHDHMDDMDDEDDDDH
VDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESD
ELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSV
VYGLRSKSKKFRRPDIQVNPLTDTPDGSD*RSS
LGNHSLHIHSFIHPFIHPFSKNSFIFYFMTIEYK
SFSAWRFL*VYIISL*I*FSNTILLFEILDHLF
SD*NKWKTPII
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRTHLRSRIS*PHRMLCPL
KKPMTLNKRPFQVSPTKAMTTWMIWMMKMMMTM
WTARTPLTRTTLMM*MTLMILTSLMSLTILMNL
MNWSLIFPRTCQQPKFSLQLSPQ*THMMAEVIVW
FMD*GQNLRSFADLTSR*IL*QTHLMVLTSAQV
*ETTVCIFIHSFIHSFIHSARIHSYSTL*PLNT
NLFLLGGFCKST*FLSRFDSQTQFYFLKSWITYF
QIKINGKHQLF
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GPISEAESPSPTECCVL
*RNQ*L*TRDPSK*VQRKP*PHG*YG**R***PC
GQPGLH*LERL**CR*H**FSPV**VSPF**I*
*TGH*FSHGPASNRSFHSSCPHSRHI*WPR**C
GLWTEVKI*EVSQT*HPGKSFNRHT*WF*LALKS
RKPQFAYSFIHSSIHSSIQQEFIHILLYDH*IQ
IFFCLAVFVSLHNFSLDLILKHNSTF*NPGSLI
FRLK*MENTNY
Sequence SPP1.jAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCACTAAAGATGTACCTACCCCTCCACAACAGATGAAACTGTG
CCAGCCAAACAACAAATGGGCATTGTCCCCAGAAGCTTGGACAAAAAGGCACACAGAGTTCAATTCCAGTTGAACAGAATAAAGGCCAAAATAGAGCTGC
CTTGGGGGTCACTGCAATTAGACTGCTTAATGAAGACATTAAAAGAACTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACCCTGACCCATC
TCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAACGAAAGCCAT
GACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATG
ATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGT
TGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGGTAAAT
CCTTTAACAGACACACCTGATGGTTCTGACTAGCGCTCAAGTCTAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTCATCCATTCA
GCAAGAATTCATTCATATTCTACTTTATGACCATTGAATACAAATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTAGATTTGATT
CTCAAACACAATTCTACTTTTTGAAATCCTGGATCACTTATTTTCAGATTAAAATAAATGGAAAACACCAATTATTT
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQH*RCTYPSTTDETV
PAKQQMGIVPRSLDKKAHRVQFQLNRIKAKIEL
PWGSLQLDCLMKTLKELYNKYPDAVATWLNPDPS
QKQNLLAPQNAVSSEETNDFKQETLPSKSNESH
DHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTD
DSHQSDESHHSDESDELVTDFPTDLPATEVFTPV
VPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQVN
PLTDTPDGSD*RSSLGNHSLHIHSFIHPFIHPF
SKNSFIFYFMTIEYKSFSAWRFL*VYIISL*I*F
SNTILLFEILDHLFSD*NKWKTPII
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSTKDVPTPPQQMKLC
QPNNKWALSPEAWTKRHTEFNSS*TE*RPK*SC
LGGHCN*TA**RH*KNFTTNTQMLWPHG*TLTH
LRSRIS*PHRMLCPLKKPMTLNKRPFQVSPTKAM
TTWMIWMMKMMMTMWTARTPLTRTTLMM*MTLM
ILTSLMSLTILMNLMNWSLIFPRTCQQPKFSLQ
LSPQ*THMMAEVIVWFMD*GQNLRSFADLTSR*I
L*QTHLMVLTSAQV*ETTVCIFIHSFIHSFIHS
ARIHSYSTL*PLNTNLFLLGGFCKST*FLSRFD
SQTQFYFLKSWITYFQIKINGKHQLF
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALKMYLPLHNR*NC
ASQTTNGHCPQKLGQKGTQSSIPVEQNKGQNRAA
LGVTAIRLLNEDIKRTLQQIPRCCGHMAKP*PI
SEAESPSPTECCVL*RNQ*L*TRDPSK*VQRKP
*PHG*YG**R***PCGQPGLH*LERL**CR*H**
FSPV**VSPF**I**TGH*FSHGPASNRSFHSS
CPHSRHI*WPR**CGLWTEVKI*EVSQT*HPGK
SFNRHT*WF*LALKSRKPQFAYSFIHSSIHSSIQ
QEFIHILLYDH*IQIFFCLAVFVSLHNFSLDLI
LKHNSTF*NPGSLIFRLK*MENTNY
Sequence SPP1.iAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC
CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCA
ACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGA
TGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTT
TTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCGGACATCACCTCACACATGGAAAGCGAGG
AGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCT
GGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGT
CAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAAC
ACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGA
AAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGT
TTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATC
ACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGT
CACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGT
GGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAATAAAACATAACCTTTTTTACTGCCTATATAA
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN
PDPSQKQNLLAPQNAVSSEETNDFKQETLPSKS
NESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD
DTDDSHQSDESHHSDESDELVTDFPTDLPATEV
FTPVVPTVDTYDGRGDSVVYGLRSDITSHMESE
ELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQL
DDQSAETHSHKQSRLYKRKANDESNEHSDVIDS
QELSKVSREFHSHEFHSHEDMLVVDPKSKEEDK
HLKFRISHELDSASSEVN*KEKKYNFSLCI*SKE
KMLYSKMKENMKCFFLSLLVECVSI*VWK*LMC
LIISLVCGFMETPCKLKASGLCLCSFYRRNANY
HCILIFVILS*IEIYVEANKILLPT*KENITFYV
TIIFCFLS*CIFCCDYLFVV*INLLS*M**EFG
GVNCLFVFPRLSSN**NITFFTAYI
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T
LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSP
TKAMTTWMIWMMKMMMTMWTARTPLTRTTLMM*
MTLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF
SLQLSPQ*THMMAEVIVWFMD*GRTSPHTWKAR
S*MVHTRPSPLPRT*TRLLIGTAVGRTVMKRVS
WMTRVLKPTATSSPDYISGKPMMRAMSIPM*LIV
RNFPKSAVNSTAMNFTAMKICWL*TPKVRKKIN
T*NFVFLMN*IVHLLRSIKRRKNTISHFAFSQK
KKCFIAK*KRT*NASFSVYWLNVYLFESGNN*CV
**LV*FVASWKLPVN*KLQGYVYVHSIEEMQTI
TVF*YLLFSHE*KFM*KQTKYFYPLKKRI*HFM
SL*SFVF*VSVYFVVIIFLWCE*IFYLECNKNLV
VSIAYLFSHGCPAINKT*PFLLPI*
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK
P*PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ
RKP*PHG*YG**R***PCGQPGLH*LERL**CR
*H**FSPV**VSPF**I**TGH*FSHGPASNRS
FHSSCPHSRHI*WPR**CGLWTEVGHHLTHGKRG
VEWCIQGHPRCPGPERAF*LGQPWEGQL*NESA
G*PEC*NPQPQAVQII*AESQ**EQ*AFRCD**
SGTFQSQP*IPQP*ISQP*RYAGCRPQK*GRR*T
PEISYFS*IR*CIF*GQLKGEKIQFLTLHLVKR
KNAL*QNEREHEMLLSQFIG*MCIYLSLEITNV
FDN*FSLWLHGNSL*TKSFRVMSMFIL*KKCKLS
LYFNICYSLMNRNLCRSKQNTFTHLKREYNILC
HYNLLFFKLVYILL*LSFCGVNKSFILNVIRIW
WCQLLICFPTVVQQLIKHNLFYCLY
Sequence SPP1.gAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCACTAAAGATGTACCTACCCCTCCACAACAGATGAAACTGTG
CCAGCCAAACAACAAATGGGCATTGTCCCCAGAAGCTTGGACAAAAAGGCACACAGAGTTCAATTCCAGTTGAACAGAATAAAGGCCAAAATAGAGCTGC
CTTGGGGGTCACTGCAATTAGACTGCTTAATGAAGACATTAAAAGAAGTATTCTGTCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACCC
TGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAAC
GAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATG
ACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTT
CACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATC
CAGGTAAATCCTTTAACAGACACACCTGATGGTTCTGACTAGCGCTCAAGTCTAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTC
ATCCATTCAGCAAGAATTCATTCATATTCTACTTTATGACCATTGAATACAAATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTA
GATTTGATTCTCAAACACAATTCTACTTTTTGAAATCCTGGATCACTTATTTTCAGATTAAAATAAATGGAAAACACCAATTATTT
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQH*RCTYPSTTDETV
PAKQQMGIVPRSLDKKAHRVQFQLNRIKAKIEL
PWGSLQLDCLMKTLKEVFCLYNKYPDAVATWLNP
DPSQKQNLLAPQNAVSSEETNDFKQETLPSKSN
ESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD
DTDDSHQSDESHHSDESDELVTDFPTDLPATEVF
TPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDI
QVNPLTDTPDGSD*RSSLGNHSLHIHSFIHPFI
HPFSKNSFIFYFMTIEYKSFSAWRFL*VYIISL*
I*FSNTILLFEILDHLFSD*NKWKTPII
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSTKDVPTPPQQMKLC
QPNNKWALSPEAWTKRHTEFNSS*TE*RPK*SC
LGGHCN*TA**RH*KKYSVFTTNTQMLWPHG*T
LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSPT
KAMTTWMIWMMKMMMTMWTARTPLTRTTLMM*M
TLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF
SLQLSPQ*THMMAEVIVWFMD*GQNLRSFADLTS
R*IL*QTHLMVLTSAQV*ETTVCIFIHSFIHSF
IHSARIHSYSTL*PLNTNLFLLGGFCKST*FLS
RFDSQTQFYFLKSWITYFQIKINGKHQLF
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALKMYLPLHNR*NC
ASQTTNGHCPQKLGQKGTQSSIPVEQNKGQNRAA
LGVTAIRLLNEDIKRSILSLQQIPRCCGHMAKP
*PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ
RKP*PHG*YG**R***PCGQPGLH*LERL**CR*
H**FSPV**VSPF**I**TGH*FSHGPASNRSF
HSSCPHSRHI*WPR**CGLWTEVKI*EVSQT*H
PGKSFNRHT*WF*LALKSRKPQFAYSFIHSSIHS
SIQQEFIHILLYDH*IQIFFCLAVFVSLHNFSL
DLILKHNSTF*NPGSLIFRLK*MENTNY
Sequence SPP1.aAug05
CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG
CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC
CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCA
ACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGA
TGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTT
TTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACA
TCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAA
CGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTA
TATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTC
ACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGA
GGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTT
CTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAAC
TAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGT
AGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGAT
TATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAAT
AAAACATAACCTTTTTTACTGCCTATATAA
- SPP1-3 (forward primer)
- SPP1-4 (reverse primer)
- Single exon skipping
Frame 0
LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVGTRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG
ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN
PDPSQKQNLLAPQNAVSSEETNDFKQETLPSKS
NESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD
DTDDSHQSDESHHSDESDELVTDFPTDLPATEV
FTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPD
IQYPDATDEDITSHMESEELNGAYKAIPVAQDLN
APSDWDSRGKDSYETSQLDDQSAETHSHKQSRL
YKRKANDESNEHSDVIDSQELSKVSREFHSHEF
HSHEDMLVVDPKSKEEDKHLKFRISHELDSASSE
VN*KEKKYNFSLCI*SKEKMLYSKMKENMKCFF
LSLLVECVSI*VWK*LMCLIISLVCGFMETPCK
LKASGLCLCSFYRRNANYHCILIFVILS*IEIYV
EANKILLPT*KENITFYVTIIFCFLS*CIFCCD
YLFVV*INLLS*M**EFGGVNCLFVFPRLSSN*
*NITFFTAYI
Frame 1
SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSGPDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS*
ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T
LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSP
TKAMTTWMIWMMKMMMTMWTARTPLTRTTLMM*
MTLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF
SLQLSPQ*THMMAEVIVWFMD*GQNLRSFADLT
SSTLMLQTRTSPHTWKARS*MVHTRPSPLPRT*
TRLLIGTAVGRTVMKRVSWMTRVLKPTATSSPDY
ISGKPMMRAMSIPM*LIVRNFPKSAVNSTAMNF
TAMKICWL*TPKVRKKINT*NFVFLMN*IVHLL
RSIKRRKNTISHFAFSQKKKCFIAK*KRT*NASF
SVYWLNVYLFESGNN*CV**LV*FVASWKLPVN
*KLQGYVYVHSIEEMQTITVF*YLLFSHE*KFM
*KQTKYFYPLKKRI*HFMSL*SFVF*VSVYFVVI
IFLWCE*IFYLECNKNLVVSIAYLFSHGCPAIN
KT*PFLLPI*
Frame 2
PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRDQTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR
HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK
P*PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ
RKP*PHG*YG**R***PCGQPGLH*LERL**CR
*H**FSPV**VSPF**I**TGH*FSHGPASNRS
FHSSCPHSRHI*WPR**CGLWTEVKI*EVSQT*H
PVP*CYRRGHHLTHGKRGVEWCIQGHPRCPGPE
RAF*LGQPWEGQL*NESAG*PEC*NPQPQAVQI
I*AESQ**EQ*AFRCD**SGTFQSQP*IPQP*IS
QP*RYAGCRPQK*GRR*TPEISYFS*IR*CIF*
GQLKGEKIQFLTLHLVKRKNAL*QNEREHEMLL
SQFIG*MCIYLSLEITNVFDN*FSLWLHGNSL*T
KSFRVMSMFIL*KKCKLSLYFNICYSLMNRNLC
RSKQNTFTHLKREYNILCHYNLLFFKLVYILL*
LSFCGVNKSFILNVIRIWWCQLLICFPTVVQQLI
KHNLFYCLY
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