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Sequences

Sequence SPP1.dAug05

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGA CCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGTCCAACGAAAGCCATGACC ACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTC TCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTC CCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGTACCCTGATG CTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTG GGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCC AATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATA TGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGA GAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTCTCAGTTTATTGGTT GAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGT TATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGTAGAAGCAAACAAAAT ACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATTATCTTTTTGTGGTG TGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAATAAAACATAACCTTTT TTACTGCCTATATAA

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN PDPSQKQNLLAPQNAVSSEETNDFKQESNESHD HMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDS HQSDESHHSDESDELVTDFPTDLPATEVFTPVV PTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPD ATDEDITSHMESEELNGAYKAIPVAQDLNAPSDW DSRGKDSYETSQLDDQSAETHSHKQSRLYKRKA NDESNEHSDVIDSQELSKVSREFHSHEFHSHED MLVVDPKSKEEDKHLKFRISHELDSASSEVN*KE KKYNFSLCI*SKEKMLYSKMKENMKCFFLSLLV ECVSI*VWK*LMCLIISLVCGFMETPCKLKASG LCLCSFYRRNANYHCILIFVILS*IEIYVEANKI LLPT*KENITFYVTIIFCFLS*CIFCCDYLFVV *INLLS*M**EFGGVNCLFVFPRLSSN**NITF FTAYI

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T LTHLRSRIS*PHRMLCPLKKPMTLNKSPTKAMT TWMIWMMKMMMTMWTARTPLTRTTLMM*MTLMI LTSLMSLTILMNLMNWSLIFPRTCQQPKFSLQLS PQ*THMMAEVIVWFMD*GQNLRSFADLTSSTLM LQTRTSPHTWKARS*MVHTRPSPLPRT*TRLLI GTAVGRTVMKRVSWMTRVLKPTATSSPDYISGKP MMRAMSIPM*LIVRNFPKSAVNSTAMNFTAMKI CWL*TPKVRKKINT*NFVFLMN*IVHLLRSIKR RKNTISHFAFSQKKKCFIAK*KRT*NASFSVYWL NVYLFESGNN*CV**LV*FVASWKLPVN*KLQG YVYVHSIEEMQTITVF*YLLFSHE*KFM*KQTK YFYPLKKRI*HFMSL*SFVF*VSVYFVVIIFLWC E*IFYLECNKNLVVSIAYLFSHGCPAINKT*PF LLPI*

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK P*PISEAESPSPTECCVL*RNQ*L*TRVQRKP*P HG*YG**R***PCGQPGLH*LERL**CR*H**F SPV**VSPF**I**TGH*FSHGPASNRSFHSSC PHSRHI*WPR**CGLWTEVKI*EVSQT*HPVP*C YRRGHHLTHGKRGVEWCIQGHPRCPGPERAF*L GQPWEGQL*NESAG*PEC*NPQPQAVQII*AES Q**EQ*AFRCD**SGTFQSQP*IPQP*ISQP*RY AGCRPQK*GRR*TPEISYFS*IR*CIF*GQLKG EKIQFLTLHLVKRKNAL*QNEREHEMLLSQFIG *MCIYLSLEITNVFDN*FSLWLHGNSL*TKSFRV MSMFIL*KKCKLSLYFNICYSLMNRNLCRSKQN TFTHLKREYNILCHYNLLFFKLVYILL*LSFCG VNKSFILNVIRIWWCQLLICFPTVVQQLIKHNLF YCLY

Sequence SPP1.lAug05

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEDPSQKQNLLAPQNAVSS EETNDFKQETLPSKSNESHDHMDDMDDEDDDDH VDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESD ELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSV VYGLRSKSKKFRRPDIQVNPLTDTPDGSD*RSS LGNHSLHIHSFIHPFIHPFSKNSFIFYFMTIEYK SFSAWRFL*VYIISL*I*FSNTILLFEILDHLF SD*NKWKTPII

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRTHLRSRIS*PHRMLCPL KKPMTLNKRPFQVSPTKAMTTWMIWMMKMMMTM WTARTPLTRTTLMM*MTLMILTSLMSLTILMNL MNWSLIFPRTCQQPKFSLQLSPQ*THMMAEVIVW FMD*GQNLRSFADLTSR*IL*QTHLMVLTSAQV *ETTVCIFIHSFIHSFIHSARIHSYSTL*PLNT NLFLLGGFCKST*FLSRFDSQTQFYFLKSWITYF QIKINGKHQLF

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GPISEAESPSPTECCVL *RNQ*L*TRDPSK*VQRKP*PHG*YG**R***PC GQPGLH*LERL**CR*H**FSPV**VSPF**I* *TGH*FSHGPASNRSFHSSCPHSRHI*WPR**C GLWTEVKI*EVSQT*HPGKSFNRHT*WF*LALKS RKPQFAYSFIHSSIHSSIQQEFIHILLYDH*IQ IFFCLAVFVSLHNFSLDLILKHNSTF*NPGSLI FRLK*MENTNY

Sequence SPP1.jAug05

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGA CCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCACTAAAGATGTACCTACCCCTCCACAACAGATGAAACTGTG CCAGCCAAACAACAAATGGGCATTGTCCCCAGAAGCTTGGACAAAAAGGCACACAGAGTTCAATTCCAGTTGAACAGAATAAAGGCCAAAATAGAGCTGC CTTGGGGGTCACTGCAATTAGACTGCTTAATGAAGACATTAAAAGAACTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACCCTGACCCATC TCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAACGAAAGCCAT GACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATG ATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGT TGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGGTAAAT CCTTTAACAGACACACCTGATGGTTCTGACTAGCGCTCAAGTCTAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTCATCCATTCA GCAAGAATTCATTCATATTCTACTTTATGACCATTGAATACAAATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTAGATTTGATT CTCAAACACAATTCTACTTTTTGAAATCCTGGATCACTTATTTTCAGATTAAAATAAATGGAAAACACCAATTATTT

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEEKQH*RCTYPSTTDETV PAKQQMGIVPRSLDKKAHRVQFQLNRIKAKIEL PWGSLQLDCLMKTLKELYNKYPDAVATWLNPDPS QKQNLLAPQNAVSSEETNDFKQETLPSKSNESH DHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTD DSHQSDESHHSDESDELVTDFPTDLPATEVFTPV VPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQVN PLTDTPDGSD*RSSLGNHSLHIHSFIHPFIHPF SKNSFIFYFMTIEYKSFSAWRFL*VYIISL*I*F SNTILLFEILDHLFSD*NKWKTPII

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRKSSTKDVPTPPQQMKLC QPNNKWALSPEAWTKRHTEFNSS*TE*RPK*SC LGGHCN*TA**RH*KNFTTNTQMLWPHG*TLTH LRSRIS*PHRMLCPLKKPMTLNKRPFQVSPTKAM TTWMIWMMKMMMTMWTARTPLTRTTLMM*MTLM ILTSLMSLTILMNLMNWSLIFPRTCQQPKFSLQ LSPQ*THMMAEVIVWFMD*GQNLRSFADLTSR*I L*QTHLMVLTSAQV*ETTVCIFIHSFIHSFIHS ARIHSYSTL*PLNTNLFLLGGFCKST*FLSRFD SQTQFYFLKSWITYFQIKINGKHQLF

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GKAALKMYLPLHNR*NC ASQTTNGHCPQKLGQKGTQSSIPVEQNKGQNRAA LGVTAIRLLNEDIKRTLQQIPRCCGHMAKP*PI SEAESPSPTECCVL*RNQ*L*TRDPSK*VQRKP *PHG*YG**R***PCGQPGLH*LERL**CR*H** FSPV**VSPF**I**TGH*FSHGPASNRSFHSS CPHSRHI*WPR**CGLWTEVKI*EVSQT*HPGK SFNRHT*WF*LALKSRKPQFAYSFIHSSIHSSIQ QEFIHILLYDH*IQIFFCLAVFVSLHNFSLDLI LKHNSTF*NPGSLIFRLK*MENTNY

Sequence SPP1.iAug05

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGA CCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCA ACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGA TGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTT TTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCGGACATCACCTCACACATGGAAAGCGAGG AGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCT GGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGT CAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAAC ACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGA AAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGT TTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATC ACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGT CACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGT GGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAATAAAACATAACCTTTTTTACTGCCTATATAA

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN PDPSQKQNLLAPQNAVSSEETNDFKQETLPSKS NESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD DTDDSHQSDESHHSDESDELVTDFPTDLPATEV FTPVVPTVDTYDGRGDSVVYGLRSDITSHMESE ELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQL DDQSAETHSHKQSRLYKRKANDESNEHSDVIDS QELSKVSREFHSHEFHSHEDMLVVDPKSKEEDK HLKFRISHELDSASSEVN*KEKKYNFSLCI*SKE KMLYSKMKENMKCFFLSLLVECVSI*VWK*LMC LIISLVCGFMETPCKLKASGLCLCSFYRRNANY HCILIFVILS*IEIYVEANKILLPT*KENITFYV TIIFCFLS*CIFCCDYLFVV*INLLS*M**EFG GVNCLFVFPRLSSN**NITFFTAYI

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSP TKAMTTWMIWMMKMMMTMWTARTPLTRTTLMM* MTLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF SLQLSPQ*THMMAEVIVWFMD*GRTSPHTWKAR S*MVHTRPSPLPRT*TRLLIGTAVGRTVMKRVS WMTRVLKPTATSSPDYISGKPMMRAMSIPM*LIV RNFPKSAVNSTAMNFTAMKICWL*TPKVRKKIN T*NFVFLMN*IVHLLRSIKRRKNTISHFAFSQK KKCFIAK*KRT*NASFSVYWLNVYLFESGNN*CV **LV*FVASWKLPVN*KLQGYVYVHSIEEMQTI TVF*YLLFSHE*KFM*KQTKYFYPLKKRI*HFM SL*SFVF*VSVYFVVIIFLWCE*IFYLECNKNLV VSIAYLFSHGCPAINKT*PFLLPI*

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK P*PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ RKP*PHG*YG**R***PCGQPGLH*LERL**CR *H**FSPV**VSPF**I**TGH*FSHGPASNRS FHSSCPHSRHI*WPR**CGLWTEVGHHLTHGKRG VEWCIQGHPRCPGPERAF*LGQPWEGQL*NESA G*PEC*NPQPQAVQII*AESQ**EQ*AFRCD** SGTFQSQP*IPQP*ISQP*RYAGCRPQK*GRR*T PEISYFS*IR*CIF*GQLKGEKIQFLTLHLVKR KNAL*QNEREHEMLLSQFIG*MCIYLSLEITNV FDN*FSLWLHGNSL*TKSFRVMSMFIL*KKCKLS LYFNICYSLMNRNLCRSKQNTFTHLKREYNILC HYNLLFFKLVYILL*LSFCGVNKSFILNVIRIW WCQLLICFPTVVQQLIKHNLFYCLY

Sequence SPP1.gAug05

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGA CCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCACTAAAGATGTACCTACCCCTCCACAACAGATGAAACTGTG CCAGCCAAACAACAAATGGGCATTGTCCCCAGAAGCTTGGACAAAAAGGCACACAGAGTTCAATTCCAGTTGAACAGAATAAAGGCCAAAATAGAGCTGC CTTGGGGGTCACTGCAATTAGACTGCTTAATGAAGACATTAAAAGAAGTATTCTGTCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACCC TGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAAC GAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATG ACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTT CACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATC CAGGTAAATCCTTTAACAGACACACCTGATGGTTCTGACTAGCGCTCAAGTCTAGGAAACCACAGTTTGCATATTCATTCATTCATTCATCCATTCATTC ATCCATTCAGCAAGAATTCATTCATATTCTACTTTATGACCATTGAATACAAATCTTTTTCTGCTTGGCGGTTTTTGTAAGTCTACATAATTTCTCTCTA GATTTGATTCTCAAACACAATTCTACTTTTTGAAATCCTGGATCACTTATTTTCAGATTAAAATAAATGGAAAACACCAATTATTT

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEEKQH*RCTYPSTTDETV PAKQQMGIVPRSLDKKAHRVQFQLNRIKAKIEL PWGSLQLDCLMKTLKEVFCLYNKYPDAVATWLNP DPSQKQNLLAPQNAVSSEETNDFKQETLPSKSN ESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD DTDDSHQSDESHHSDESDELVTDFPTDLPATEVF TPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDI QVNPLTDTPDGSD*RSSLGNHSLHIHSFIHPFI HPFSKNSFIFYFMTIEYKSFSAWRFL*VYIISL* I*FSNTILLFEILDHLFSD*NKWKTPII

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRKSSTKDVPTPPQQMKLC QPNNKWALSPEAWTKRHTEFNSS*TE*RPK*SC LGGHCN*TA**RH*KKYSVFTTNTQMLWPHG*T LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSPT KAMTTWMIWMMKMMMTMWTARTPLTRTTLMM*M TLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF SLQLSPQ*THMMAEVIVWFMD*GQNLRSFADLTS R*IL*QTHLMVLTSAQV*ETTVCIFIHSFIHSF IHSARIHSYSTL*PLNTNLFLLGGFCKST*FLS RFDSQTQFYFLKSWITYFQIKINGKHQLF

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GKAALKMYLPLHNR*NC ASQTTNGHCPQKLGQKGTQSSIPVEQNKGQNRAA LGVTAIRLLNEDIKRSILSLQQIPRCCGHMAKP *PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ RKP*PHG*YG**R***PCGQPGLH*LERL**CR* H**FSPV**VSPF**I**TGH*FSHGPASNRSF HSSCPHSRHI*WPR**CGLWTEVKI*EVSQT*H PGKSFNRHT*WF*LALKSRKPQFAYSFIHSSIHS SIQQEFIHILLYDH*IQIFFCLAVFVSLHNFSL DLILKHNSTF*NPGSLIFRLK*MENTNY

Sequence SPP1.aAug05

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGGGAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGTCGGGA CCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGG CATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAAC CCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCA ACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGA TGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTT TTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACA TCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAA CGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTA TATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTC ACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGA GGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTT CTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAAC TAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATTTATGT AGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGAT TATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAATTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAAT AAAACATAACCTTTTTTACTGCCTATATAA

SPP1-3 (forward primer)
SPP1-4 (reverse primer)
Alternative acceptor

Frame 0

LPVLVEDVCSSI*ILGGLGCQQQQEEAEHSIVG TRLVSGQLQPSQPNADQGKLTTMRIAVICFCLLG ITCAIPVKQADSGSSEEKQLYNKYPDAVATWLN PDPSQKQNLLAPQNAVSSEETNDFKQETLPSKS NESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVD DTDDSHQSDESHHSDESDELVTDFPTDLPATEV FTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPD IQYPDATDEDITSHMESEELNGAYKAIPVAQDLN APSDWDSRGKDSYETSQLDDQSAETHSHKQSRL YKRKANDESNEHSDVIDSQELSKVSREFHSHEF HSHEDMLVVDPKSKEEDKHLKFRISHELDSASSE VN*KEKKYNFSLCI*SKEKMLYSKMKENMKCFF LSLLVECVSI*VWK*LMCLIISLVCGFMETPCK LKASGLCLCSFYRRNANYHCILIFVILS*IEIYV EANKILLPT*KENITFYVTIIFCFLS*CIFCCD YLFVV*INLLS*M**EFGGVNCLFVFPRLSSN* *NITFFTAYI

Frame 1

SLCWWRMSAAAFKFWEGLVVSSSRRRQSTASSG PDSSQASCSLLSQTPTKENSLP*ELQ*FAFAS* ASPVPYQLNRLILEVLRKSSFTTNTQMLWPHG*T LTHLRSRIS*PHRMLCPLKKPMTLNKRPFQVSP TKAMTTWMIWMMKMMMTMWTARTPLTRTTLMM* MTLMILTSLMSLTILMNLMNWSLIFPRTCQQPKF SLQLSPQ*THMMAEVIVWFMD*GQNLRSFADLT SSTLMLQTRTSPHTWKARS*MVHTRPSPLPRT* TRLLIGTAVGRTVMKRVSWMTRVLKPTATSSPDY ISGKPMMRAMSIPM*LIVRNFPKSAVNSTAMNF TAMKICWL*TPKVRKKINT*NFVFLMN*IVHLL RSIKRRKNTISHFAFSQKKKCFIAK*KRT*NASF SVYWLNVYLFESGNN*CV**LV*FVASWKLPVN *KLQGYVYVHSIEEMQTITVF*YLLFSHE*KFM *KQTKYFYPLKKRI*HFMSL*SFVF*VSVYFVVI IFLWCE*IFYLECNKNLVVSIAYLFSHGCPAIN KT*PFLLPI*

Frame 2

PCVGGGCLQQHLNSGRAWLSAAAGGGRAQHRRD QTRLRPVAAFSAKRRPRKTHYHENCSDLLLPPR HHLCHTS*TG*FWKF*GKAALQQIPRCCGHMAK P*PISEAESPSPTECCVL*RNQ*L*TRDPSK*VQ RKP*PHG*YG**R***PCGQPGLH*LERL**CR *H**FSPV**VSPF**I**TGH*FSHGPASNRS FHSSCPHSRHI*WPR**CGLWTEVKI*EVSQT*H PVP*CYRRGHHLTHGKRGVEWCIQGHPRCPGPE RAF*LGQPWEGQL*NESAG*PEC*NPQPQAVQI I*AESQ**EQ*AFRCD**SGTFQSQP*IPQP*IS QP*RYAGCRPQK*GRR*TPEISYFS*IR*CIF* GQLKGEKIQFLTLHLVKRKNAL*QNEREHEMLL SQFIG*MCIYLSLEITNVFDN*FSLWLHGNSL*T KSFRVMSMFIL*KKCKLSLYFNICYSLMNRNLC RSKQNTFTHLKREYNILCHYNLLFFKLVYILL* LSFCGVNKSFILNVIRIWWCQLLICFPTVVQQLI KHNLFYCLY