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Sequences

Sequence PRSS25.bAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG
AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC
CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC
ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT
CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT
CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG
ATCCTGG
ACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG
TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT
GAGGATTCAGACTAAGTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCC
TTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGG
TGATGATGCTGACCCTGAGTCCCAGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGA
AGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCAC
CAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTC
CCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATG
AGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCC
TATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGC
CTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATG
GTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAG
GGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR
S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV*
PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS
LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT
LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS
SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR
SW
TGTLSWAARSLSRTAQDSWWLPMGSLSPTPM
WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR
*GFRLSLETLEVPWLTWMGR*LE*TP*RSQLESP
LPSLLIVFESFCIVGKRRIPPPESVGPSGATLG
**C*P*VPGLVCGLVM*FWPLGSRWYKMLKMFM
KLFEPNPSWQCRSGGDEKH*PYM*PLRSQNE*IT
KSMRLLL*FPPCLSG*GSEGTETEG*MNQWGQV
PPTTSTDSWALKNHRNTFYIK*NYT*QHIIVKN
EVGGLDLFPHQKARGKAVSP*T*GRYWS*PS*PP
IKENELLPSFVGS*SGAALCGVVGSGLFCSVLG
LGAKIPMLGYRY*QLASEGG*KLLLDSSTSIVH
GLMSAVADMGFLVSS*GNGSLRPLPLGASGGLQR
AVALR*SGCWSGQGSVQTSCWPLH*RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG
ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD
P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG
H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL
WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP
RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR
DPG
PAPFLGPRGPYLERLRIRGGCRWAHCHQRPC
GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA
EDSD*VWKLWRSPG*PGWGGDWSEHHEGHSWNL
LCHPF*SSSRVSASWGKEEFLLRNQWVPAALHWG
DDADPESQGWSAAW*CDFGHWGADGTKC*RCL*
SCSNPIPVGSADPAGTRNTDLICDP*GHRMNRS
PRV*GSCSDFLLAFLAEVLRAPRQRVK*TSGGRS
LQPPALTPGL*RITETLFI*NKIIPSNIL**KM
RWEGWIFSPTKRLEVKLYPPKLRGDTGADHPDL
LLKKMSCCHLLWAVSQVLLFVVWWALVCSARCWA
WEQRFPCLATDTDSWPLKEGENFCLTVPHP*CM
V**VRLLTWVSW*APEVMAASDPCH*GPVVVCR
GQWHLDNLVAGLARVAFKPPVGLFTEGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA
ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT
PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV
TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN
SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL
AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE
IL
DRHPFLGREVPISNGSGFVVAADGLIVTNAH
VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL
RIQTKFGNSGGPLVNLDGEVIGVNTMKVTAGIS
FAIPSDRLREFLHRGEKKNSSSGISGSQRRYIG
VMMLTLSPRAGLRPGDVILAIGEQMVQNAEDVYE
AVRTQSQLAVQIRRGRETLTLYVTPEVTE*IDH
QEYEAPALISSLPFWLRF*GHRDRGLNEPVGAG
PSNHQH*LLGSEESQKHFLYKIKLYLATYYSKK*
GGRAGSFPPPKG*R*SCIPLNLGEILELTILTS
Y*RK*AAAIFCGQLVRCCSLWCGGLWSVLLGAG
PGSKDSHAWLQILTAGL*RRVKTSA*QFHIHSAW
SDECGC*HGFLGKLLR*WQPQTPAIRGQWWFAE
GSGT*IIWLLVWPG*RSNLLLASSLKAS

Sequence PRSS25.cAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG
AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC
CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC
ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT
CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT
CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG
ATCCTGG
ACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG
TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT
GAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA
CTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATG
CAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGCTAGGGAACTGGGGGCTGTATCCCTGCAGGATGGGGAGGTGATTGGAGTGAACAC
CATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGT
GGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGG
TACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCC
TGAG
GTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGG
TTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACC
TAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGAT
ACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTC
TGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTT
GACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCAT
TAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTG
AAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR
S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV*
PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS
LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT
LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS
SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR
SW
TGTLSWAARSLSRTAQDSWWLPMGSLSPTPM
WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR
*GFRLRSLSPRCLWDAQLMSGKGSLLLPWEVPLH
CRTRSHPALLALLSVQPETWDSPKPMWNTFKLM
QLLILETLEVPWLTWLGNWGLYPCRMGR*LE*T
P*RSQLESPLPSLLIVFESFCIVGKRRIPPPESV
GPSGATLG**C*P*VPGLVCGLVM*FWPLGSRW
YKMLKMFMKLFEPNPSWQCRSGGDEKH*PYM*P
LR
SQNE*ITKSMRLLL*FPPCLSG*GSEGTETEG
*MNQWGQVPPTTSTDSWALKNHRNTFYIK*NYT
*QHIIVKNEVGGLDLFPHQKARGKAVSP*T*GR
YWS*PS*PPIKENELLPSFVGS*SGAALCGVVGS
GLFCSVLGLGAKIPMLGYRY*QLASEGG*KLLL
DSSTSIVHGLMSAVADMGFLVSS*GNGSLRPLP
LGASGGLQRAVALR*SGCWSGQGSVQTSCWPLH*
RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG
ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD
P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG
H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL
WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP
RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR
DPG
PAPFLGPRGPYLERLRIRGGCRWAHCHQRPC
GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA
EDSD*GASPHAASGTLS*CPARGVCCCHGKSLC
TAEHDHIRHC*LCSASSQRPGTPPNQCGIHSN*C
SY*FWKLWRSPG*PG*GTGGCIPAGWGGDWSEH
HEGHSWNLLCHPF*SSSRVSASWGKEEFLLRNQ
WVPAALHWGDDADPESQGWSAAW*CDFGHWGADG
TKC*RCL*SCSNPIPVGSADPAGTRNTDLICDP
*
GHRMNRSPRV*GSCSDFLLAFLAEVLRAPRQR
VK*TSGGRSLQPPALTPGL*RITETLFI*NKIIP
SNIL**KMRWEGWIFSPTKRLEVKLYPPKLRGD
TGADHPDLLLKKMSCCHLLWAVSQVLLFVVWWA
LVCSARCWAWEQRFPCLATDTDSWPLKEGENFCL
TVPHP*CMV**VRLLTWVSW*APEVMAASDPCH
*GPVVVCRGQWHLDNLVAGLARVAFKPPVGLFT
EGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA
ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT
PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV
TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN
SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL
AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE
IL
DRHPFLGREVPISNGSGFVVAADGLIVTNAH
VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL
RIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFA
LQNTITSGIVSSAQRPARDLGLPQTNVEYIQTD
AAIDFGNSGGPLVNLARELGAVSLQDGEVIGVNT
MKVTAGISFAIPSDRLREFLHRGEKKNSSSGIS
GSQRRYIGVMMLTLSPRAGLRPGDVILAIGEQM
VQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTP
E
VTE*IDHQEYEAPALISSLPFWLRF*GHRDRG
LNEPVGAGPSNHQH*LLGSEESQKHFLYKIKLY
LATYYSKK*GGRAGSFPPPKG*R*SCIPLNLGEI
LELTILTSY*RK*AAAIFCGQLVRCCSLWCGGL
WSVLLGAGPGSKDSHAWLQILTAGL*RRVKTSA
*QFHIHSAWSDECGC*HGFLGKLLR*WQPQTPAI
RGQWWFAEGSGT*IIWLLVWPG*RSNLLLASSL
KAS

Sequence PRSS25.gAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCC
GCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGT
GAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCC
ACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTG
TTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGG
TCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTT
CTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTG
CTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGTGAGGGAGAGG
CTGCAGTGTGATATGGGGATGGGCAAGGTGTGCATGTGTCCTTGAACTAGGCTTTGTACTCCTTCCTTTCTCTCTGTCCATTTTTCTCTATAG
GGCTGGT
CTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGC
AGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTC
CTTGCCTTTCTGGCTGAGGT
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSVSTTSSQMWWRRQHLPWSISRSWTGTLSWA
ARSLSRTAQDSWWLPMGSLSPTPMWWLIGAESV
*DC*AATRMRPWSQLWIPWQTSQR*GFRLRSLSP
RCLWDAQLMSGKGSLLLPWEVPLHCRTRSHPAL
LALLSVQPETWDSPKPMWNTFKLMQLLILETLE
VPWLTWMGR*LE*TP*RSQLESPLPSLLIVFESF
CIVGKRRIPPPESVGPSGATLG**C*P*VPASL
LNYSFENQAFPMFSMVYSSIKSSWAPLHTGEGE
AAV*YGDGQGVHVSLN*ALYSFLSLCPFFSI
GLV
CGLVM*FWPLGSRWYKMLKMFMKLFEPNPSWQC
RSGGDEKH*PYM*PLRSQNE*ITKSMRLLL*FP
PCLSG*G

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQSVQLHRRCGGEDSTCRGLYRDPGPAPFLGP
RGPYLERLRIRGGCRWAHCHQRPCGG*SAQSPC
ETAKRRHV*GRGHSCGSRGRHRNAEDSD*GASP
HAASGTLS*CPARGVCCCHGKSLCTAEHDHIRHC
*LCSASSQRPGTPPNQCGIHSN*CSY*FWKLWR
SPG*PGWGGDWSEHHEGHSWNLLCHPF*SSSRV
SASWGKEEFLLRNQWVPAALHWGDDADPESQHPC
*TTASRTKLSRCSAWCTHP*SHPGLPCTPVRER
LQCDMGMGKVCMCP*TRLCTPSFLSVHFSL*
GW
SAAW*CDFGHWGADGTKC*RCL*SCSNPIPVGSA
DPAGTRNTDLICDP*GHRMNRSPRV*GSCSDFL
LAFLAE

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFSQYNFIADVVEKTAPAVVYIEILDRHPFLG
REVPISNGSGFVVAADGLIVTNAHVVADRRRVRV
RLLSGDTYEAVVTAVDPVADIATLRIQTKEPLP
TLPLGRSADVRQGEFVVAMGSPFALQNTITSGI
VSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGG
PLVNLDGEVIGVNTMKVTAGISFAIPSDRLREF
LHRGEKKNSSSGISGSQRRYIGVMMLTLSPSIL
AELQLREPSFPDVQHGVLIHKVILGSPAHR*GRG
CSVIWGWARCACVLELGFVLLPFSLSIFLYR
AG
LRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAV
QIRRGRETLTLYVTPEVTE*IDHQEYEAPALISS
LPFWLR

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