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Sequences

Sequence PRSS25.eAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG ATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT GAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA CTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATG CAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGTGAGTGAGACATCCTTCCTTCCAAGAATCCCTGCCCCAGGTCAGTGTGGGAAGGG TAGGTTTCCCCTAATTCAAGGATGTTTGGTCAAGTTTCTGAGCAGTTCTTTGTTGGCTATCTCTCAATATCCAACCAGATCTCCCCAACACTTGCTGGTA CTTTTGTTCGGGTGCCCCCATCCCCTACTATTTGTTTAGGCTAGGGAACTGGGGGCTGTATCCCTGCAGGATGGGGAGGTGATTGGAGTGAACACCATGA AGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTC CCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGT GTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATG CTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCAC AGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAA CCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACAT ATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCT GACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTT CTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCC ACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCA GTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCA CC

PRSS25-3 (forward primer)
PRSS25-4 (reverse primer)
Intron retention

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW AVSQLASGGDHNSRHSWGREESASRNPGPGVHF* RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV* PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR SWTGTLSWAARSLSRTAQDSWWLPMGSLSPTPM WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR *GFRLRSLSPRCLWDAQLMSGKGSLLLPWEVPLH CRTRSHPALLALLSVQPETWDSPKPMWNTFKLM QLLILETLEVPWLTW*VRHPSFQESLPQVSVGR VGFP*FKDVWSSF*AVLCWLSLNIQPDLPNTCWY FCSGAPIPYYLFRLGNWGLYPCRMGR*LE*TP* RSQLESPLPSLLIVFESFCIVGKRRIPPPESVG PSGATLG**C*P*VPASLLNYSFENQAFPMFSMV YSSIKSSWAPLHTGLVCGLVM*FWPLGSRWYKM LKMFMKLFEPNPSWQCRSGGDEKH*PYM*PLRS QNE*ITKSMRLLL*FPPCLSG*GSEGTETEG*MN QWGQVPPTTSTDSWALKNHRNTFYIK*NYT*QH IIVKNEVGGLDLFPHQKARGKAVSP*T*GRYWS *PS*PPIKENELLPSFVGS*SGAALCGVVGSGLF CSVLGLGAKIPMLGYRY*QLASEGG*KLLLDSS TSIVHGLMSAVADMGFLVSS*GNGSLRPLPLGA SGGLQRAVALR*SGCWSGQGSVQTSCWPLH*RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR DPGPAPFLGPRGPYLERLRIRGGCRWAHCHQRPC GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA EDSD*GASPHAASGTLS*CPARGVCCCHGKSLC TAEHDHIRHC*LCSASSQRPGTPPNQCGIHSN*C SY*FWKLWRSPG*PGE*DILPSKNPCPRSVWEG *VSPNSRMFGQVSEQFFVGYLSISNQISPTLAG TFVRVPPSPTICLG*GTGGCIPAGWGGDWSEHHE GHSWNLLCHPF*SSSRVSASWGKEEFLLRNQWV PAALHWGDDADPESQHPC*TTASRTKLSRCSAW CTHP*SHPGLPCTPGWSAAW*CDFGHWGADGTKC *RCL*SCSNPIPVGSADPAGTRNTDLICDP*GH RMNRSPRV*GSCSDFLLAFLAEVLRAPRQRVK* TSGGRSLQPPALTPGL*RITETLFI*NKIIPSNI L**KMRWEGWIFSPTKRLEVKLYPPKLRGDTGA DHPDLLLKKMSCCHLLWAVSQVLLFVVWWALVC SARCWAWEQRFPCLATDTDSWPLKEGENFCLTVP HP*CMV**VRLLTWVSW*APEVMAASDPCH*GP VVVCRGQWHLDNLVAGLARVAFKPPVGLFTEGI T

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE ILDRHPFLGREVPISNGSGFVVAADGLIVTNAH VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL RIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFA LQNTITSGIVSSAQRPARDLGLPQTNVEYIQTD AAIDFGNSGGPLVNLVSETSFLPRIPAPGQCGKG RFPLIQGCLVKFLSSSLLAISQYPTRSPQHLLV LLFGCPHPLLFV*ARELGAVSLQDGEVIGVNTM KVTAGISFAIPSDRLREFLHRGEKKNSSSGISGS QRRYIGVMMLTLSPSILAELQLREPSFPDVQHG VLIHKVILGSPAHRAGLRPGDVILAIGEQMVQN AEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVT E*IDHQEYEAPALISSLPFWLRF*GHRDRGLNE PVGAGPSNHQH*LLGSEESQKHFLYKIKLYLAT YYSKK*GGRAGSFPPPKG*R*SCIPLNLGEILEL TILTSY*RK*AAAIFCGQLVRCCSLWCGGLWSV LLGAGPGSKDSHAWLQILTAGL*RRVKTSA*QF HIHSAWSDECGC*HGFLGKLLR*WQPQTPAIRGQ WWFAEGSGT*IIWLLVWPG*RSNLLLASSLKAS

Sequence PRSS25.fAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG AAGGACTTCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAAC GGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACA CGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTC AGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCA GCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATG GGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAA GAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAA CCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGG CCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACT GACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGA GGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTAT ATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCC CCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCT TTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGA GGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCA GCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTC CTGTTGGCCTCTTCACTGAAGGCATCACC

PRSS25-3 (forward primer)
PRSS25-4 (reverse primer)
Intron retention

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW AVSQLASGGDHNSRHSWGREESASRNPGPGVHF* RTSDVVEKTAPAVVYIEILDRHPFLGREVPISN GSGFVVAADGLIVTNAHVVADRRRVRVRLLSGD TYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRS ADVRQGEFVVAMGSPFALQNTITSGIVSSAQRP ARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLD GEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKK NSSSGISGSQRRYIGVMMLTLSPSILAELQLRE PSFPDVQHGVLIHKVILGSPAHRAGLRPGDVIL AIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETL TLYVTPEVTE*IDHQEYEAPALISSLPFWLRF* GHRDRGLNEPVGAGPSNHQH*LLGSEESQKHFL YKIKLYLATYYSKK*GGRAGSFPPPKG*R*SCIP LNLGEILELTILTSY*RK*AAAIFCGQLVRCCS LWCGGLWSVLLGAGPGSKDSHAWLQILTAGL*R RVKTSA*QFHIHSAWSDECGC*HGFLGKLLR*WQ PQTPAIRGQWWFAEGSGT*IIWLLVWPG*RSNL LLASSLKAS

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS EGLQMWWRRQHLPWSISRSWTGTLSWAARSLSRT AQDSWWLPMGSLSPTPMWWLIGAESV*DC*AAT RMRPWSQLWIPWQTSQR*GFRLRSLSPRCLWDA QLMSGKGSLLLPWEVPLHCRTRSHPALLALLSVQ PETWDSPKPMWNTFKLMQLLILETLEVPWLTWM GR*LE*TP*RSQLESPLPSLLIVFESFCIVGKR RIPPPESVGPSGATLG**C*P*VPASLLNYSFEN QAFPMFSMVYSSIKSSWAPLHTGLVCGLVM*FW PLGSRWYKMLKMFMKLFEPNPSWQCRSGGDEKH *PYM*PLRSQNE*ITKSMRLLL*FPPCLSG*GSE GTETEG*MNQWGQVPPTTSTDSWALKNHRNTFY IK*NYT*QHIIVKNEVGGLDLFPHQKARGKAVS P*T*GRYWS*PS*PPIKENELLPSFVGS*SGAAL CGVVGSGLFCSVLGLGAKIPMLGYRY*QLASEG G*KLLLDSSTSIVHGLMSAVADMGFLVSS*GNG SLRPLPLGASGGLQRAVALR*SGCWSGQGSVQTS CWPLH*RHH

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL KDFRCGGEDSTCRGLYRDPGPAPFLGPRGPYLE RLRIRGGCRWAHCHQRPCGG*SAQSPCETAKRRH V*GRGHSCGSRGRHRNAEDSD*GASPHAASGTL S*CPARGVCCCHGKSLCTAEHDHIRHC*LCSAS SQRPGTPPNQCGIHSN*CSY*FWKLWRSPG*PGW GGDWSEHHEGHSWNLLCHPF*SSSRVSASWGKE EFLLRNQWVPAALHWGDDADPESQHPC*TTASR TKLSRCSAWCTHP*SHPGLPCTPGWSAAW*CDFG HWGADGTKC*RCL*SCSNPIPVGSADPAGTRNT DLICDP*GHRMNRSPRV*GSCSDFLLAFLAEVL RAPRQRVK*TSGGRSLQPPALTPGL*RITETLFI *NKIIPSNIL**KMRWEGWIFSPTKRLEVKLYP PKLRGDTGADHPDLLLKKMSCCHLLWAVSQVLL FVVWWALVCSARCWAWEQRFPCLATDTDSWPLKE GENFCLTVPHP*CMV**VRLLTWVSW*APEVMA ASDPCH*GPVVVCRGQWHLDNLVAGLARVAFKP PVGLFTEGIT

Sequence PRSS25.dAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG AAGGACTTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGA GGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGA CTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGC TGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAG CTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCC CTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGC ATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGA ACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCT GGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGC GGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTT CTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCA CAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGA GGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTT AGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACA GCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGC TCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGG GTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC

PRSS25-3 (forward primer)
PRSS25-4 (reverse primer)
Intron retention

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW AVSQLASGGDHNSRHSWGREESASRNPGPGVHF* RTSVQLHRRCGGEDSTCRGLYRDPGPAPFLGPR GPYLERLRIRGGCRWAHCHQRPCGG*SAQSPCE TAKRRHV*GRGHSCGSRGRHRNAEDSD*GASPHA ASGTLS*CPARGVCCCHGKSLCTAEHDHIRHC* LCSASSQRPGTPPNQCGIHSN*CSY*FWKLWRS PG*PGWGGDWSEHHEGHSWNLLCHPF*SSSRVSA SWGKEEFLLRNQWVPAALHWGDDADPESQHPC* TTASRTKLSRCSAWCTHP*SHPGLPCTPGWSAA W*CDFGHWGADGTKC*RCL*SCSNPIPVGSADPA GTRNTDLICDP*GHRMNRSPRV*GSCSDFLLAF LAEVLRAPRQRVK*TSGGRSLQPPALTPGL*RI TETLFI*NKIIPSNIL**KMRWEGWIFSPTKRLE VKLYPPKLRGDTGADHPDLLLKKMSCCHLLWAV SQVLLFVVWWALVCSARCWAWEQRFPCLATDTD SWPLKEGENFCLTVPHP*CMV**VRLLTWVSW*A PEVMAASDPCH*GPVVVCRGQWHLDNLVAGLAR VAFKPPVGLFTEGIT

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS EGLQYNFIADVVEKTAPAVVYIEILDRHPFLGRE VPISNGSGFVVAADGLIVTNAHVVADRRRVRVR LLSGDTYEAVVTAVDPVADIATLRIQTKEPLPT LPLGRSADVRQGEFVVAMGSPFALQNTITSGIVS SAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGP LVNLDGEVIGVNTMKVTAGISFAIPSDRLREFL HRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAE LQLREPSFPDVQHGVLIHKVILGSPAHRAGLRP GDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIR RGRETLTLYVTPEVTE*IDHQEYEAPALISSLPF WLRF*GHRDRGLNEPVGAGPSNHQH*LLGSEES QKHFLYKIKLYLATYYSKK*GGRAGSFPPPKG* R*SCIPLNLGEILELTILTSY*RK*AAAIFCGQL VRCCSLWCGGLWSVLLGAGPGSKDSHAWLQILT AGL*RRVKTSA*QFHIHSAWSDECGC*HGFLGK LLR*WQPQTPAIRGQWWFAEGSGT*IIWLLVWPG *RSNLLLASSLKAS

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL KDFSTTSSQMWWRRQHLPWSISRSWTGTLSWAA RSLSRTAQDSWWLPMGSLSPTPMWWLIGAESV*D C*AATRMRPWSQLWIPWQTSQR*GFRLRSLSPR CLWDAQLMSGKGSLLLPWEVPLHCRTRSHPALL ALLSVQPETWDSPKPMWNTFKLMQLLILETLEVP WLTWMGR*LE*TP*RSQLESPLPSLLIVFESFC IVGKRRIPPPESVGPSGATLG**C*P*VPASLL NYSFENQAFPMFSMVYSSIKSSWAPLHTGLVCGL VM*FWPLGSRWYKMLKMFMKLFEPNPSWQCRSG GDEKH*PYM*PLRSQNE*ITKSMRLLL*FPPCL SG*GSEGTETEG*MNQWGQVPPTTSTDSWALKNH RNTFYIK*NYT*QHIIVKNEVGGLDLFPHQKAR GKAVSP*T*GRYWS*PS*PPIKENELLPSFVGS *SGAALCGVVGSGLFCSVLGLGAKIPMLGYRY*Q LASEGG*KLLLDSSTSIVHGLMSAVADMGFLVS S*GNGSLRPLPLGASGGLQRAVALR*SGCWSGQ GSVQTSCWPLH*RHH

Sequence PRSS25.aAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG ATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT GAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA CTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATG CAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGC CATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATG ATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGG GCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCG AACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTAT GAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACC ACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAG GGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGA AAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCA AAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGA GTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGC ACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC

PRSS25-3 (forward primer)
PRSS25-4 (reverse primer)
Intron retention

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW AVSQLASGGDHNSRHSWGREESASRNPGPGVHF* RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV* PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR SWTGTLSWAARSLSRTAQDSWWLPMGSLSPTPM WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR *GFRLRSLSPRCLWDAQLMSGKGSLLLPWEVPLH CRTRSHPALLALLSVQPETWDSPKPMWNTFKLM QLLILETLEVPWLTWMGR*LE*TP*RSQLESPL PSLLIVFESFCIVGKRRIPPPESVGPSGATLG** C*P*VPASLLNYSFENQAFPMFSMVYSSIKSSW APLHTGLVCGLVM*FWPLGSRWYKMLKMFMKLF EPNPSWQCRSGGDEKH*PYM*PLRSQNE*ITKSM RLLL*FPPCLSG*GSEGTETEG*MNQWGQVPPT TSTDSWALKNHRNTFYIK*NYT*QHIIVKNEVG GLDLFPHQKARGKAVSP*T*GRYWS*PS*PPIKE NELLPSFVGS*SGAALCGVVGSGLFCSVLGLGA KIPMLGYRY*QLASEGG*KLLLDSSTSIVHGLM SAVADMGFLVSS*GNGSLRPLPLGASGGLQRAVA LR*SGCWSGQGSVQTSCWPLH*RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR DPGPAPFLGPRGPYLERLRIRGGCRWAHCHQRPC GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA EDSD*GASPHAASGTLS*CPARGVCCCHGKSLC TAEHDHIRHC*LCSASSQRPGTPPNQCGIHSN*C SY*FWKLWRSPG*PGWGGDWSEHHEGHSWNLLC HPF*SSSRVSASWGKEEFLLRNQWVPAALHWGD DADPESQHPC*TTASRTKLSRCSAWCTHP*SHPG LPCTPGWSAAW*CDFGHWGADGTKC*RCL*SCS NPIPVGSADPAGTRNTDLICDP*GHRMNRSPRV *GSCSDFLLAFLAEVLRAPRQRVK*TSGGRSLQP PALTPGL*RITETLFI*NKIIPSNIL**KMRWE GWIFSPTKRLEVKLYPPKLRGDTGADHPDLLLK KMSCCHLLWAVSQVLLFVVWWALVCSARCWAWEQ RFPCLATDTDSWPLKEGENFCLTVPHP*CMV** VRLLTWVSW*APEVMAASDPCH*GPVVVCRGQW HLDNLVAGLARVAFKPPVGLFTEGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE ILDRHPFLGREVPISNGSGFVVAADGLIVTNAH VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL RIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFA LQNTITSGIVSSAQRPARDLGLPQTNVEYIQTD AAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFA IPSDRLREFLHRGEKKNSSSGISGSQRRYIGVM MLTLSPSILAELQLREPSFPDVQHGVLIHKVIL GSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVR TQSQLAVQIRRGRETLTLYVTPEVTE*IDHQEY EAPALISSLPFWLRF*GHRDRGLNEPVGAGPSN HQH*LLGSEESQKHFLYKIKLYLATYYSKK*GGR AGSFPPPKG*R*SCIPLNLGEILELTILTSY*R K*AAAIFCGQLVRCCSLWCGGLWSVLLGAGPGS KDSHAWLQILTAGL*RRVKTSA*QFHIHSAWSDE CGC*HGFLGKLLR*WQPQTPAIRGQWWFAEGSG T*IIWLLVWPG*RSNLLLASSLKAS

Sequence PRSS25.gAug05

PRSS25-3 (forward primer)
PRSS25-4 (reverse primer)
Intron retention

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW AVSQLASGGDHNSRHSWGREESASRNPGPGVHF* RTSVSTTSSQMWWRRQHLPWSISRSWTGTLSWA ARSLSRTAQDSWWLPMGSLSPTPMWWLIGAESV *DC*AATRMRPWSQLWIPWQTSQR*GFRLRSLSP RCLWDAQLMSGKGSLLLPWEVPLHCRTRSHPAL LALLSVQPETWDSPKPMWNTFKLMQLLILETLE VPWLTWMGR*LE*TP*RSQLESPLPSLLIVFESF CIVGKRRIPPPESVGPSGATLG**C*P*VPASL LNYSFENQAFPMFSMVYSSIKSSWAPLHTGEGE AAV*YGDGQGVHVSLN*ALYSFLSLCPFFSIGLV CGLVM*FWPLGSRWYKMLKMFMKLFEPNPSWQC RSGGDEKH*PYM*PLRSQNE*ITKSMRLLL*FP PCLSG*G

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS EGLQSVQLHRRCGGEDSTCRGLYRDPGPAPFLGP RGPYLERLRIRGGCRWAHCHQRPCGG*SAQSPC ETAKRRHV*GRGHSCGSRGRHRNAEDSD*GASP HAASGTLS*CPARGVCCCHGKSLCTAEHDHIRHC *LCSASSQRPGTPPNQCGIHSN*CSY*FWKLWR SPG*PGWGGDWSEHHEGHSWNLLCHPF*SSSRV SASWGKEEFLLRNQWVPAALHWGDDADPESQHPC *TTASRTKLSRCSAWCTHP*SHPGLPCTPVRER LQCDMGMGKVCMCP*TRLCTPSFLSVHFSL*GW SAAW*CDFGHWGADGTKC*RCL*SCSNPIPVGSA DPAGTRNTDLICDP*GHRMNRSPRV*GSCSDFL LAFLAE

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL KDFSQYNFIADVVEKTAPAVVYIEILDRHPFLG REVPISNGSGFVVAADGLIVTNAHVVADRRRVRV RLLSGDTYEAVVTAVDPVADIATLRIQTKEPLP TLPLGRSADVRQGEFVVAMGSPFALQNTITSGI VSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGG PLVNLDGEVIGVNTMKVTAGISFAIPSDRLREF LHRGEKKNSSSGISGSQRRYIGVMMLTLSPSIL AELQLREPSFPDVQHGVLIHKVILGSPAHR*GRG CSVIWGWARCACVLELGFVLLPFSLSIFLYRAG LRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAV QIRRGRETLTLYVTPEVTE*IDHQEYEAPALISS LPFWLR