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Sequences

Sequence PRSS25.bAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG
AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC
CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC
ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT
CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT
CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG
ATCCTGG
ACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG
TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT
GAGGATTCAGACTAAGTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCC
TTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGG
TGATGATGCTGACCCTGAGTCCCAGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGA
AGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCAC
CAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTC
CCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATG
AGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCC
TATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGC
CTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATG
GTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAG
GGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR
S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV*
PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS
LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT
LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS
SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR
SW
TGTLSWAARSLSRTAQDSWWLPMGSLSPTPM
WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR
*GFRLSLETLEVPWLTWMGR*LE*TP*RSQLESP
LPSLLIVFESFCIVGKRRIPPPESVGPSGATLG
**C*P*VPGLVCGLVM*FWPLGSRWYKMLKMFM
KLFEPNPSWQCRSGGDEKH*PYM*PLRSQNE*IT
KSMRLLL*FPPCLSG*GSEGTETEG*MNQWGQV
PPTTSTDSWALKNHRNTFYIK*NYT*QHIIVKN
EVGGLDLFPHQKARGKAVSP*T*GRYWS*PS*PP
IKENELLPSFVGS*SGAALCGVVGSGLFCSVLG
LGAKIPMLGYRY*QLASEGG*KLLLDSSTSIVH
GLMSAVADMGFLVSS*GNGSLRPLPLGASGGLQR
AVALR*SGCWSGQGSVQTSCWPLH*RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG
ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD
P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG
H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL
WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP
RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR
DPG
PAPFLGPRGPYLERLRIRGGCRWAHCHQRPC
GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA
EDSD*VWKLWRSPG*PGWGGDWSEHHEGHSWNL
LCHPF*SSSRVSASWGKEEFLLRNQWVPAALHWG
DDADPESQGWSAAW*CDFGHWGADGTKC*RCL*
SCSNPIPVGSADPAGTRNTDLICDP*GHRMNRS
PRV*GSCSDFLLAFLAEVLRAPRQRVK*TSGGRS
LQPPALTPGL*RITETLFI*NKIIPSNIL**KM
RWEGWIFSPTKRLEVKLYPPKLRGDTGADHPDL
LLKKMSCCHLLWAVSQVLLFVVWWALVCSARCWA
WEQRFPCLATDTDSWPLKEGENFCLTVPHP*CM
V**VRLLTWVSW*APEVMAASDPCH*GPVVVCR
GQWHLDNLVAGLARVAFKPPVGLFTEGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA
ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT
PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV
TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN
SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL
AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE
IL
DRHPFLGREVPISNGSGFVVAADGLIVTNAH
VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL
RIQTKFGNSGGPLVNLDGEVIGVNTMKVTAGIS
FAIPSDRLREFLHRGEKKNSSSGISGSQRRYIG
VMMLTLSPRAGLRPGDVILAIGEQMVQNAEDVYE
AVRTQSQLAVQIRRGRETLTLYVTPEVTE*IDH
QEYEAPALISSLPFWLRF*GHRDRGLNEPVGAG
PSNHQH*LLGSEESQKHFLYKIKLYLATYYSKK*
GGRAGSFPPPKG*R*SCIPLNLGEILELTILTS
Y*RK*AAAIFCGQLVRCCSLWCGGLWSVLLGAG
PGSKDSHAWLQILTAGL*RRVKTSA*QFHIHSAW
SDECGC*HGFLGKLLR*WQPQTPAIRGQWWFAE
GSGT*IIWLLVWPG*RSNLLLASSLKAS

Sequence PRSS25.fAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAAC
GGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACA
CGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTC
AGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCA
GCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATG
GGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAA
GAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAA
CCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGG
CCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACT
GACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGA
GGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTAT
ATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCC
CCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCT
TTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGA
GGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCA
GCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTC
CTGTTGGCCTCTTCACTGAAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSDVVEKTAPAVVYIEILDRHPFLGREVPISN
GSGFVVAADGLIVTNAHVVADRRRVRVRLLSGD
TYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRS
ADVRQGEFVVAMGSPFALQNTITSGIVSSAQRP
ARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLD
GEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKK
NSSSGISGSQRRYIGVMMLTLSPSILAELQLRE
PSFPDVQHGVLIHKVILGSPAHRAGLRPGDVIL
AIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETL
TLYVTPEVTE*IDHQEYEAPALISSLPFWLRF*
GHRDRGLNEPVGAGPSNHQH*LLGSEESQKHFL
YKIKLYLATYYSKK*GGRAGSFPPPKG*R*SCIP
LNLGEILELTILTSY*RK*AAAIFCGQLVRCCS
LWCGGLWSVLLGAGPGSKDSHAWLQILTAGL*R
RVKTSA*QFHIHSAWSDECGC*HGFLGKLLR*WQ
PQTPAIRGQWWFAEGSGT*IIWLLVWPG*RSNL
LLASSLKAS

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQMWWRRQHLPWSISRSWTGTLSWAARSLSRT
AQDSWWLPMGSLSPTPMWWLIGAESV*DC*AAT
RMRPWSQLWIPWQTSQR*GFRLRSLSPRCLWDA
QLMSGKGSLLLPWEVPLHCRTRSHPALLALLSVQ
PETWDSPKPMWNTFKLMQLLILETLEVPWLTWM
GR*LE*TP*RSQLESPLPSLLIVFESFCIVGKR
RIPPPESVGPSGATLG**C*P*VPASLLNYSFEN
QAFPMFSMVYSSIKSSWAPLHTGLVCGLVM*FW
PLGSRWYKMLKMFMKLFEPNPSWQCRSGGDEKH
*PYM*PLRSQNE*ITKSMRLLL*FPPCLSG*GSE
GTETEG*MNQWGQVPPTTSTDSWALKNHRNTFY
IK*NYT*QHIIVKNEVGGLDLFPHQKARGKAVS
P*T*GRYWS*PS*PPIKENELLPSFVGS*SGAAL
CGVVGSGLFCSVLGLGAKIPMLGYRY*QLASEG
G*KLLLDSSTSIVHGLMSAVADMGFLVSS*GNG
SLRPLPLGASGGLQRAVALR*SGCWSGQGSVQTS
CWPLH*RHH

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRCGGEDSTCRGLYRDPGPAPFLGPRGPYLE
RLRIRGGCRWAHCHQRPCGG*SAQSPCETAKRRH
V*GRGHSCGSRGRHRNAEDSD*GASPHAASGTL
S*CPARGVCCCHGKSLCTAEHDHIRHC*LCSAS
SQRPGTPPNQCGIHSN*CSY*FWKLWRSPG*PGW
GGDWSEHHEGHSWNLLCHPF*SSSRVSASWGKE
EFLLRNQWVPAALHWGDDADPESQHPC*TTASR
TKLSRCSAWCTHP*SHPGLPCTPGWSAAW*CDFG
HWGADGTKC*RCL*SCSNPIPVGSADPAGTRNT
DLICDP*GHRMNRSPRV*GSCSDFLLAFLAEVL
RAPRQRVK*TSGGRSLQPPALTPGL*RITETLFI
*NKIIPSNIL**KMRWEGWIFSPTKRLEVKLYP
PKLRGDTGADHPDLLLKKMSCCHLLWAVSQVLL
FVVWWALVCSARCWAWEQRFPCLATDTDSWPLKE
GENFCLTVPHP*CMV**VRLLTWVSW*APEVMA
ASDPCH*GPVVVCRGQWHLDNLVAGLARVAFKP
PVGLFTEGIT

Sequence PRSS25.dAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGA
GGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGA
CTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGC
TGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAG
CTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCC
CTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGC
ATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGA
ACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCT
GGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGC
GGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTT
CTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCA
CAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGA
GGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTT
AGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACA
GCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGC
TCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGG
GTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSVQLHRRCGGEDSTCRGLYRDPGPAPFLGPR
GPYLERLRIRGGCRWAHCHQRPCGG*SAQSPCE
TAKRRHV*GRGHSCGSRGRHRNAEDSD*GASPHA
ASGTLS*CPARGVCCCHGKSLCTAEHDHIRHC*
LCSASSQRPGTPPNQCGIHSN*CSY*FWKLWRS
PG*PGWGGDWSEHHEGHSWNLLCHPF*SSSRVSA
SWGKEEFLLRNQWVPAALHWGDDADPESQHPC*
TTASRTKLSRCSAWCTHP*SHPGLPCTPGWSAA
W*CDFGHWGADGTKC*RCL*SCSNPIPVGSADPA
GTRNTDLICDP*GHRMNRSPRV*GSCSDFLLAF
LAEVLRAPRQRVK*TSGGRSLQPPALTPGL*RI
TETLFI*NKIIPSNIL**KMRWEGWIFSPTKRLE
VKLYPPKLRGDTGADHPDLLLKKMSCCHLLWAV
SQVLLFVVWWALVCSARCWAWEQRFPCLATDTD
SWPLKEGENFCLTVPHP*CMV**VRLLTWVSW*A
PEVMAASDPCH*GPVVVCRGQWHLDNLVAGLAR
VAFKPPVGLFTEGIT

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQYNFIADVVEKTAPAVVYIEILDRHPFLGRE
VPISNGSGFVVAADGLIVTNAHVVADRRRVRVR
LLSGDTYEAVVTAVDPVADIATLRIQTKEPLPT
LPLGRSADVRQGEFVVAMGSPFALQNTITSGIVS
SAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGP
LVNLDGEVIGVNTMKVTAGISFAIPSDRLREFL
HRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAE
LQLREPSFPDVQHGVLIHKVILGSPAHRAGLRP
GDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIR
RGRETLTLYVTPEVTE*IDHQEYEAPALISSLPF
WLRF*GHRDRGLNEPVGAGPSNHQH*LLGSEES
QKHFLYKIKLYLATYYSKK*GGRAGSFPPPKG*
R*SCIPLNLGEILELTILTSY*RK*AAAIFCGQL
VRCCSLWCGGLWSVLLGAGPGSKDSHAWLQILT
AGL*RRVKTSA*QFHIHSAWSDECGC*HGFLGK
LLR*WQPQTPAIRGQWWFAEGSGT*IIWLLVWPG
*RSNLLLASSLKAS

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFSTTSSQMWWRRQHLPWSISRSWTGTLSWAA
RSLSRTAQDSWWLPMGSLSPTPMWWLIGAESV*D
C*AATRMRPWSQLWIPWQTSQR*GFRLRSLSPR
CLWDAQLMSGKGSLLLPWEVPLHCRTRSHPALL
ALLSVQPETWDSPKPMWNTFKLMQLLILETLEVP
WLTWMGR*LE*TP*RSQLESPLPSLLIVFESFC
IVGKRRIPPPESVGPSGATLG**C*P*VPASLL
NYSFENQAFPMFSMVYSSIKSSWAPLHTGLVCGL
VM*FWPLGSRWYKMLKMFMKLFEPNPSWQCRSG
GDEKH*PYM*PLRSQNE*ITKSMRLLL*FPPCL
SG*GSEGTETEG*MNQWGQVPPTTSTDSWALKNH
RNTFYIK*NYT*QHIIVKNEVGGLDLFPHQKAR
GKAVSP*T*GRYWS*PS*PPIKENELLPSFVGS
*SGAALCGVVGSGLFCSVLGLGAKIPMLGYRY*Q
LASEGG*KLLLDSSTSIVHGLMSAVADMGFLVS
S*GNGSLRPLPLGASGGLQRAVALR*SGCWSGQ
GSVQTSCWPLH*RHH

Sequence PRSS25.aAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG
AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC
CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC
ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT
CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT
CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG
ATCCTGG
ACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG
TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT
GAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA
CTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATG
CAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGC
CATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATG
ATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGG
GCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCG
AACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTAT
GAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGGTTAAATGAACCAGTGGGGGCAGGTCCCTCCAACC
ACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACCTAGCAACATATTATAGTAAAAAATGAGGTGGGAG
GGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGATACTGGAGCTGACCATCCTGACCTCCTATTAAAGA
AAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTCTGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCA
AAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTTGACAGTTCCACATCCATAGTGCATGGTCTGATGA
GTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCATTAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGC
ACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTGAAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR
S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV*
PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS
LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT
LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS
SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR
SW
TGTLSWAARSLSRTAQDSWWLPMGSLSPTPM
WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR
*GFRLRSLSPRCLWDAQLMSGKGSLLLPWEVPLH
CRTRSHPALLALLSVQPETWDSPKPMWNTFKLM
QLLILETLEVPWLTWMGR*LE*TP*RSQLESPL
PSLLIVFESFCIVGKRRIPPPESVGPSGATLG**
C*P*VPASLLNYSFENQAFPMFSMVYSSIKSSW
APLHTGLVCGLVM*FWPLGSRWYKMLKMFMKLF
EPNPSWQCRSGGDEKH*PYM*PLRSQNE*ITKSM
RLLL*FPPCLSG*GSEGTETEG*MNQWGQVPPT
TSTDSWALKNHRNTFYIK*NYT*QHIIVKNEVG
GLDLFPHQKARGKAVSP*T*GRYWS*PS*PPIKE
NELLPSFVGS*SGAALCGVVGSGLFCSVLGLGA
KIPMLGYRY*QLASEGG*KLLLDSSTSIVHGLM
SAVADMGFLVSS*GNGSLRPLPLGASGGLQRAVA
LR*SGCWSGQGSVQTSCWPLH*RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG
ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD
P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG
H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL
WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP
RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR
DPG
PAPFLGPRGPYLERLRIRGGCRWAHCHQRPC
GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA
EDSD*GASPHAASGTLS*CPARGVCCCHGKSLC
TAEHDHIRHC*LCSASSQRPGTPPNQCGIHSN*C
SY*FWKLWRSPG*PGWGGDWSEHHEGHSWNLLC
HPF*SSSRVSASWGKEEFLLRNQWVPAALHWGD
DADPESQHPC*TTASRTKLSRCSAWCTHP*SHPG
LPCTPGWSAAW*CDFGHWGADGTKC*RCL*SCS
NPIPVGSADPAGTRNTDLICDP*GHRMNRSPRV
*GSCSDFLLAFLAEVLRAPRQRVK*TSGGRSLQP
PALTPGL*RITETLFI*NKIIPSNIL**KMRWE
GWIFSPTKRLEVKLYPPKLRGDTGADHPDLLLK
KMSCCHLLWAVSQVLLFVVWWALVCSARCWAWEQ
RFPCLATDTDSWPLKEGENFCLTVPHP*CMV**
VRLLTWVSW*APEVMAASDPCH*GPVVVCRGQW
HLDNLVAGLARVAFKPPVGLFTEGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA
ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT
PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV
TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN
SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL
AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE
IL
DRHPFLGREVPISNGSGFVVAADGLIVTNAH
VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL
RIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFA
LQNTITSGIVSSAQRPARDLGLPQTNVEYIQTD
AAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFA
IPSDRLREFLHRGEKKNSSSGISGSQRRYIGVM
MLTLSPSILAELQLREPSFPDVQHGVLIHKVIL
GSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVR
TQSQLAVQIRRGRETLTLYVTPEVTE*IDHQEY
EAPALISSLPFWLRF*GHRDRGLNEPVGAGPSN
HQH*LLGSEESQKHFLYKIKLYLATYYSKK*GGR
AGSFPPPKG*R*SCIPLNLGEILELTILTSY*R
K*AAAIFCGQLVRCCSLWCGGLWSVLLGAGPGS
KDSHAWLQILTAGL*RRVKTSA*QFHIHSAWSDE
CGC*HGFLGKLLR*WQPQTPAIRGQWWFAEGSG
T*IIWLLVWPG*RSNLLLASSLKAS

Sequence PRSS25.gAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCC
GCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGT
GAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCC
ACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTG
TTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGG
TCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTT
CTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTG
CTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGTGAGGGAGAGG
CTGCAGTGTGATATGGGGATGGGCAAGGTGTGCATGTGTCCTTGAACTAGGCTTTGTACTCCTTCCTTTCTCTCTGTCCATTTTTCTCTATAGGGCTGGT
CTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGC
AGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTC
CTTGCCTTTCTGGCTGAGGT
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSVSTTSSQMWWRRQHLPWSISRSWTGTLSWA
ARSLSRTAQDSWWLPMGSLSPTPMWWLIGAESV
*DC*AATRMRPWSQLWIPWQTSQR*GFRLRSLSP
RCLWDAQLMSGKGSLLLPWEVPLHCRTRSHPAL
LALLSVQPETWDSPKPMWNTFKLMQLLILETLE
VPWLTWMGR*LE*TP*RSQLESPLPSLLIVFESF
CIVGKRRIPPPESVGPSGATLG**C*P*VPASL
LNYSFENQAFPMFSMVYSSIKSSWAPLHTGEGE
AAV*YGDGQGVHVSLN*ALYSFLSLCPFFSIGLV
CGLVM*FWPLGSRWYKMLKMFMKLFEPNPSWQC
RSGGDEKH*PYM*PLRSQNE*ITKSMRLLL*FP
PCLSG*G

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQSVQLHRRCGGEDSTCRGLYRDPGPAPFLGP
RGPYLERLRIRGGCRWAHCHQRPCGG*SAQSPC
ETAKRRHV*GRGHSCGSRGRHRNAEDSD*GASP
HAASGTLS*CPARGVCCCHGKSLCTAEHDHIRHC
*LCSASSQRPGTPPNQCGIHSN*CSY*FWKLWR
SPG*PGWGGDWSEHHEGHSWNLLCHPF*SSSRV
SASWGKEEFLLRNQWVPAALHWGDDADPESQHPC
*TTASRTKLSRCSAWCTHP*SHPGLPCTPVRER
LQCDMGMGKVCMCP*TRLCTPSFLSVHFSL*GW
SAAW*CDFGHWGADGTKC*RCL*SCSNPIPVGSA
DPAGTRNTDLICDP*GHRMNRSPRV*GSCSDFL
LAFLAE

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFSQYNFIADVVEKTAPAVVYIEILDRHPFLG
REVPISNGSGFVVAADGLIVTNAHVVADRRRVRV
RLLSGDTYEAVVTAVDPVADIATLRIQTKEPLP
TLPLGRSADVRQGEFVVAMGSPFALQNTITSGI
VSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGG
PLVNLDGEVIGVNTMKVTAGISFAIPSDRLREF
LHRGEKKNSSSGISGSQRRYIGVMMLTLSPSIL
AELQLREPSFPDVQHGVLIHKVILGSPAHR*GRG
CSVIWGWARCACVLELGFVLLPFSLSIFLYRAG
LRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAV
QIRRGRETLTLYVTPEVTE*IDHQEYEAPALISS
LPFWLR

Sequence PRSS25.cAug05

AGACGTGGATCCCGAGAAAGAGGCGCAGGACGAGGAGGCAGAACCCGACTGGCGCGTAGAGCAGCAGCACGAGCAGTAGGAAGCAGTCACCCGGAAGCCT
GGGGGCGAGAGGCGAAGTGGTCAGGCGCCGAAGGCCGAGAGCACGCGGGGATCGGTCTCTTCCCGCCGGGTCTCTTACCGGTGCGAGTCAAAGAGCCGCT
CCGGCCCCGGCCCTGAGGGAAGCTCCATAACTGCTGCTTCAGGAGCGCCCGGCCGTCGCCGCCGCCGCCATTTTCGCGCCCGGCCGCAGGGGCTCTTGGG
AAGGCGGAGTCTTTGGGCATCCGCCCGGGGTGAGGGGACCCGAAGTCCTGAGGCGCGCCGGAAGGGCTAGCGGTCCCAGCATACCCCGCGGCCCCTTGGG
CCGTCTCACAACTCGCGTCCGGCGGAGACCACAATTCCCGGCATTCGTGGGGCAGGGAGGAGTCGGCCTCCCGGAATCCTGGTCCCGGCGTGCACTTCTG
AAGGACTTCAGGTACCGGCGTGCCCCGCGTCCTACTGTCCGCCTGCTCGCGTCCTGGGTGCCGCCTCTGAGTAGGGCGGGCGAGGAGGCAGCCAAGGCGG
AGCTGATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGAC
CCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTC
ACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACT
CTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCT
CGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAG
ATCCTGG
ACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATG
TGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCT
GAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA
CTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATG
CAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGCTAGGGAACTGGGGGCTGTATCCCTGCAGGATGGGGAGGTGATTGGAGTGAACAC
CATGAAGGTCACAGCTGGAATCTCCTTTGCCATCCCTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGT
GGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGG
TACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCC
TGAG
GTCACAGAATGAATAGATCACCAAGAGTATGAGGCTCCTGCTCTGATTTCCTCCTTGCCTTTCTGGCTGAGGTTCTGAGGGCACCGAGACAGAGGG
TTAAATGAACCAGTGGGGGCAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAGAAACACTTTTTATATAAAATAAAATTATACC
TAGCAACATATTATAGTAAAAAATGAGGTGGGAGGGCTGGATCTTTTCCCCCACCAAAAGGCTAGAGGTAAAGCTGTATCCCCCTAAACTTAGGGGAGAT
ACTGGAGCTGACCATCCTGACCTCCTATTAAAGAAAATGAGCTGCTGCCATCTTTTGTGGGCAGTTAGTCAGGTGCTGCTCTTTGTGGTGTGGTGGGCTC
TGGTCTGTTCTGCTCGGTGCTGGGCCTGGGAGCAAAGATTCCCATGCTTGGCTACAGATACTGACAGCTGGCCTCTGAAGGAGGGTGAAAACTTCTGCTT
GACAGTTCCACATCCATAGTGCATGGTCTGATGAGTGCGGTTGCTGACATGGGTTTCTTGGTAAGCTCCTGAGGTAATGGCAGCCTCAGACCCCTGCCAT
TAGGGGCCAGTGGTGGTTTGCAGAGGGCAGTGGCACTTAGATAATCTGGTTGCTGGTCTGGCCAGGGTAGCGTTCAAACCTCCTGTTGGCCTCTTCACTG
AAGGCATCACC
  • PRSS25-3 (forward primer)
  • PRSS25-4 (reverse primer)
  • Alternative acceptor

Frame 0

RRGSRERGAGRGGRTRLARRAAARAVGSSHPEA
WGREAKWSGAEGREHAGIGLFPPGLLPVRVKEPL
RPRP*GKLHNCCFRSARPSPPPPFSRPAAGALG
KAESLGIRPG*GDPKS*GAPEGLAVPAYPAAPW
AVSQLASGGDHNSRHSWGREESASRNPGPGVHF*
RTSGTGVPRVLLSACSRPGCRL*VGRARRQPRR
S*WLRRGRGGVQAGAFGHGGLWGAFAGGGDPV*
PLTSGPC*RQELLTPGPE*LMGPPVSGPGCLLGS
LNPEHA*RLGPRVPGHN*LR*PQIPGPGRPQRT
LEPVRARGWRWRWALGGQCCCCCGAGVGVLRPS
SPPSLARRPLLPGVSTTSSQMWWRRQHLPWSISR
SW
TGTLSWAARSLSRTAQDSWWLPMGSLSPTPM
WWLIGAESV*DC*AATRMRPWSQLWIPWQTSQR
*GFRLRSLSPRCLWDAQLMSGKGSLLLPWEVPLH
CRTRSHPALLALLSVQPETWDSPKPMWNTFKLM
QLLILETLEVPWLTWLGNWGLYPCRMGR*LE*T
P*RSQLESPLPSLLIVFESFCIVGKRRIPPPESV
GPSGATLG**C*P*VPGLVCGLVM*FWPLGSRW
YKMLKMFMKLFEPNPSWQCRSGGDEKH*PYM*P
LR
SQNE*ITKSMRLLL*FPPCLSG*GSEGTETEG
*MNQWGQVPPTTSTDSWALKNHRNTFYIK*NYT
*QHIIVKNEVGGLDLFPHQKARGKAVSP*T*GR
YWS*PS*PPIKENELLPSFVGS*SGAALCGVVGS
GLFCSVLGLGAKIPMLGYRY*QLASEGG*KLLL
DSSTSIVHGLMSAVADMGFLVSS*GNGSLRPLP
LGASGGLQRAVALR*SGCWSGQGSVQTSCWPLH*
RHH

Frame 1

DVDPEKEAQDEEAEPDWRVEQQHEQ*EAVTRKP
GGERRSGQAPKAESTRGSVSSRRVSYRCESKSR
SGPGPEGSSITAASGAPGRRRRRHFRARPQGLLG
RRSLWASARGEGTRSPEARRKG*RSQHTPRPLG
PSHNSRPAETTIPGIRGAGRSRPPGILVPACTS
EGLQVPACPASYCPPARVLGAASE*GGRGGSQGG
ADGCAEGGAGCRLEPSGMAGFGGHSLGEETPFD
P*PPGPADVRNF*PPGPSDLWDPQSLGPVVCWG
H*TPSMPDVWDPGSPGTTDCGDPRYQDPGGLREL
WNPFARVAGGGAGRWGGSAVVVVGRGSGSSGRP
RRRP*PAARFSPESVQLHRRCGGEDSTCRGLYR
DPG
PAPFLGPRGPYLERLRIRGGCRWAHCHQRPC
GG*SAQSPCETAKRRHV*GRGHSCGSRGRHRNA
EDSD*GASPHAASGTLS*CPARGVCCCHGKSLC
TAEHDHIRHC*LCSASSQRPGTPPNQCGIHSN*C
SY*FWKLWRSPG*PG*GTGGCIPAGWGGDWSEH
HEGHSWNLLCHPF*SSSRVSASWGKEEFLLRNQ
WVPAALHWGDDADPESQGWSAAW*CDFGHWGADG
TKC*RCL*SCSNPIPVGSADPAGTRNTDLICDP
*
GHRMNRSPRV*GSCSDFLLAFLAEVLRAPRQR
VK*TSGGRSLQPPALTPGL*RITETLFI*NKIIP
SNIL**KMRWEGWIFSPTKRLEVKLYPPKLRGD
TGADHPDLLLKKMSCCHLLWAVSQVLLFVVWWA
LVCSARCWAWEQRFPCLATDTDSWPLKEGENFCL
TVPHP*CMV**VRLLTWVSW*APEVMAASDPCH
*GPVVVCRGQWHLDNLVAGLARVAFKPPVGLFT
EGIT

Frame 2

TWIPRKRRRTRRQNPTGA*SSSTSSRKQSPGSL
GARGEVVRRRRPRARGDRSLPAGSLTGASQRAA
PAPALREAP*LLLQERPAVAAAAIFAPGRRGSW
EGGVFGHPPGVRGPEVLRRAGRASGPSIPRGPLG
RLTTRVRRRPQFPAFVGQGGVGLPESWSRRALL
KDFRYRRAPRPTVRLLASWVPPLSRAGEEAAKA
ELMAAPRAGRGAGWSLRAWRALGGIRWGRRPRLT
PDLRALLTSGTSDPRARVTYGTPSLWARLSVGV
TEPRACLTSGTPGPRAQLTAVTPDTRTREASEN
SGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVL
AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIE
IL
DRHPFLGREVPISNGSGFVVAADGLIVTNAH
VVADRRRVRVRLLSGDTYEAVVTAVDPVADIATL
RIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFA
LQNTITSGIVSSAQRPARDLGLPQTNVEYIQTD
AAIDFGNSGGPLVNLARELGAVSLQDGEVIGVNT
MKVTAGISFAIPSDRLREFLHRGEKKNSSSGIS
GSQRRYIGVMMLTLSPRAGLRPGDVILAIGEQM
VQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTP
E
VTE*IDHQEYEAPALISSLPFWLRF*GHRDRG
LNEPVGAGPSNHQH*LLGSEESQKHFLYKIKLY
LATYYSKK*GGRAGSFPPPKG*R*SCIPLNLGEI
LELTILTSY*RK*AAAIFCGQLVRCCSLWCGGL
WSVLLGAGPGSKDSHAWLQILTAGL*RRVKTSA
*QFHIHSAWSDECGC*HGFLGKLLR*WQPQTPAI
RGQWWFAEGSGT*IIWLLVWPG*RSNLLLASSL
KAS

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