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Sequences

Sequence TRAF2.dAug05

TCGCCTAGGCCTCCCAAAGTGCTGGGATTACAGGCTGGTCTCGAACTCCTGGCCTGAAGTGATCCTCCAACCTCAGCCTCCCAAGTAGCTGGGATTACAG
GTTGAATCGCTCCTAGCTGACCCCTCACGTCTCTTATGATACGTACAGAAAGCAAGAGCAGCCCATGCCGTCCGGCCGCGCACCAGGTCTCAGTCTGTGT
GTCTGGTTCAGGTGACCATGGTGTTCAGTTCAGTTCCTCCTGCCTCGTGTGTAGGCCACTGAGAGCTCCCTAGTCTAAGCCGGAGGAGGTTCTGGGCTTT
GTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCCTGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACC
AAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGCAGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCA
GCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGGGCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGA
TAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAGTGATGGATGCACCTGGAAGGGGACCCTGAAAGAATACGAGAGCTGCCACGAA
GGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTGGTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAA
GCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGAAGGCGCACCACGAGGTCTGCCCCAAGTTCCCCTTAACTTGTGACGGCTGCGG
CAAGAAGAAGATCCCCCGGGAGAAGTTTCAGGACCACGTCAAGACTTGTGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCTGCCTCGAGACG
GTAGAGGGTGAGAAACAGCAGGAGCACGAGGTGCAGTGGCTGCGGGAGCACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAG
ACCAGAGCCACGCGGGGTCAGAGCTCCTGCAGAGGTGCGAGAGCCTGGAGAAGAAGACGGCCACTTTTGAGAACATTGTCTGCGTCCTGAACCGGGAGGT
GGAGAGGGTGGCCATGACTGCCGAGGCCTGCAGCCGGCAGCACCGGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGGTCTTGGCACCCGGC
AGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTCTTGGAGATGGAGGCATCCACCTACGATGGGGT
CTTCATCTGGAAGATCTCAGACTTCGCCAGGAAGCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACACCAGCAGGTAC
GGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCACCGGGCGAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACG
CCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGACCTTAATGCTGCTCGACCAGAATAACCGGGAGCACGTGATTGACGCCTTCAGGCCCGACGTGACTTC
ATCCTCTTTTCAGAGGCCAGTCAACGACATGAACATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATTCCTACGTGCGG
GACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGCTCTAACTGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCA
CGGAGGGGCCACCACGCTGGGCCAGGGTCTCACTGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTGGGAGGGTGTCGGCCTGCAGCCAAGTTCACTGT
CACGGGGGAAGGAGCCACCAGCCAGTCCTCAGATTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCATTGGCCGAGGG
TCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTGCAGTGAAGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAG
TGCCCATGTAGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCTGCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAG
CAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCTGGCCCCTGGAGAGAAGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGAGCTCTGGTCTGTG
CCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTCTGGTCCCACGCCGCCTCTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTG
TGAGAGCTTGCCATCCAGCTCACGAAGACAGAGTTATTAAACCATTCAAATCTCTGTGGTCAGTGGCATCCATTTGATTCCAGGTTGCCTGCGGCTTCCA
GCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCCTGGCCTCTCCTCTCACAGACCCCTCCCCACGGGGTGCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCA
GGCTGTGTTCTCTGTTCCTCACCTGATGTAAGCTCCCTCTTCTCGAGCCTTGTTCCCTCTTCTGGAATGTTCTTCCCTGTCCATCTCCTCTCCTGGGCCT
CCCCAGGGACTCCTGTGAGAGCAGAATGAGGATGAGCCAGCCCTGGTCCTGCCTTTCTGTGCACAGGCTCCACCGAGCATCCTGGCTGCACCCGGGGGGT
CCTGGCCACACCGAGGGTCCTGGCTGCACCGCCCCTGTGGTGTGTTTTTGGGACAGTGCAGGCAGGCGTAGGCACGGCTTCACACTCGGTGTCAGGGCAG
CCGTGCTGGGACCTACTGGGCATCTGTATCGGGGTCCAGGATAAAGTGCTGTCCCCTCTCGACGTCTTCACGCACTGCTGTAGTTTGAGAAGTTGACCAC
AGAAAAGTACAGAAAATAACTCAGGCACGGAGCTGACCGACGGGCATTGTCTGTGTTCCGTGAGTGTGTACTGGGCCTAAAGAGCAAGCATTGCAGAGCC
CTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTCCCCAGGCCACGTGGCGGGAATTGAGGCGTTCCGGTTCTCTTTGGGCTTTAGAATTTTGCATCTGTAC
GTTTCTGTATTCACAAATAAGAATATTGCATTAAAAATGCAGAAACCATCCACTGGGTCAGCTGCAGGTAGCTGGGCTTTTGACTCAGCCTGGCTACTTT
TAATGCATCTTGTGCTGGGCACTCTACATGGTGGATTCTGACTGCCGTGTGTGAGTTCCCTAGAGAGCACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTT
TGGTGACTGGAGATAGCCAGACCTGCTCTCGTGGGTCTCCTGTCAACCTGGAAGGAGGGTTGGTGGTTTCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCT
TGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCACCAGCACCAGGGGGAGGGTTCCCATCACTCCCATCCCTCCCATCCCTGCCCGTGCTTCGTGTTGTTG
GAATTGAGATGCTGCAGCGTGCTGTGTGTGAAGTGAAGTGGCATCGCTGTCACGTGCGTTTCTCTGATGACTCGGCGGAGTCAGGCGTCTTTACCACGCT
TACCTCTCCCTTGGATTCTCTCCCGTGCGAATTGCCTGTTGATGTCTTTTTCTCATTTTTCTTCTGGTTGTTGGTCTGGGTCTTATTCTGCTGGTTGGTA
GGAGTTCCTGATATTCCTGATTACGGATTCTCGGTTGTGTCTTTGAACTGCGTTTCTGTTGCTCCTGATGTGCAGAAGCTTAAGCCTTACACATGATCAG
CTGATCTTATCCTTTATGAATGGTGCTATTTCCGACCGACTCAGAACATCCTGACCTTCCCTGAGGCTGGAAACCACTGTCATGTTTTCGTCTAATGATT
GTGAAGTTTTGTCTTTAACATACTGGGCTTCTATATTTGGGGATTTGTTTGTATGTGACAGGTTTTGCTGGGTCACTGCAGTTTGTTAAAACAGATTGAT
TGAGGTCATCTCTTCTCACTTAAAACGAAATGGAACTGTGAAATGAGAGGAATGGACCAAAAAAAAATCCTCCCCCAGGGACAAAGATGGGGAAGAAGCC
AACATCCCACAAAGGATTTGAACCCATTTTGGAAGGTGGGAAGTGGGTTGGTGGGTGGCTGCTGAGCAGAAGAGGGCTGAGGGGGAAGCTGGCCACACCG
GGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTGGATCAACTGAAAGACGGGGCAGAGCAGCTACCTTGGAGGTCCTTTGCCAGAGCAGCCGGATGGTCCT
CCTGGCCCTCACCTCCCTGCAGTGGAGATGGAGGTCTGGAGAACCTGGGGAAGAGCTGAGGACCGCACCCCCCACGCAGTGAGAGCAGCATCGGGCTCCA
GGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAACAATTCCCTAGGCACTGGCTGCCACACCAAGAGGCTCCCACGAAGCAGACCAAGGCCCTTGAGGGAC
AAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCTGGCCCAGCCGCCTCATGGCCTTCTGGGGAAGTGACAGGCTCTGTGCAAACCCACAGAGGTACCATCG
TCACTACCTAATTTTGGGACATTTCATCACCCTGCAGAGAAACCTTCCCTTAGCAGTCACTGTTCCCTCCCGCTCAGCCCCTGACAGCCAGCCATAAACC
TACCTTCTGTCTCTATAGATTTGCCTACTCAGGACATTCATGTGAATGTGGCCTTTGCTTTTACTCAGCAAGATATTTTCATGGTTCCTGTGTGTTGCAG
CATGCATTGGAGTTTCATTCCTTCTAATGGCTGAATAATCTCTTGTATGGATGCACCACACCTTATTTATCCATTCATCCGTTGCTGGACATTTGGGTGG
TTTCTACTTTTTGGCTACTCTGAAAATGCTGCTGTGAACATTTGTGAACAAGTTTTTATGGGGACGTG
  • TRAF2-3 (forward primer)
  • TRAF2-4 (reverse primer)
  • Alternative donor

Frame 0

SPRPPKVLGLQAGLELLA*SDPPTSASQVAGIT
G*IAPS*PLTSLMIRTESKSSPCRPAAHQVSVCV
SGSGDHGVQFSSSCLVCRPLRAP*SKPEEVLGF
VRGGHSSHGCS*RDPPWLPGVATARLLQDPPGD
QAGSQVPVLRLQKRPPQALPGAVWPPVLLLLPGQ
HPQLWASELCCLCSRGHI*RRHFYFRKQFGLPR
*CCPQGGGEPAGRLSQ*WMHLEGDPERIRELPR
RPLPAHADRMSRVQRPGPPW*KGAPPGARVPGEK
PELPALPGTLLRSRREGAPRGLPQVPLNL*RLR
QEEDPPGEVSGPRQDLWQVSSPLQIPRHRLPRD
GRG*ETAGARGAVAAGAPGHATELGAGGKAPLGR
PEPRGVRAPAEVREPGEEDGHF*EHCLRPEPGG
GEGGHDCRGLQPAAPAGPRQD*SPE*QGGLGTR
QVQQLERSIGLKDLAMADLEQKVLEMEASTYDGV
FIWKISDFARKRQEAVAGRIPAIFSPAFYTSRY
GYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPND
ALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTS
SSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVR
DDAIFIKAIVDLTGL*LPPTGVWGLGAARHSRL
TEGPPRWARVSLYKWAGAALGRLGGCRPAAKFTV
TGEGATSQSSDFRDCGGAWQTVLAKGCGGIGRG
SSGASQHKLPLLSCAVKGEALGGGHSEWEHIPA
VPM*QEHSGWPCVPRA*QAETRAAPGEGPPAGQS
KEG*AAWFSPLAPGEKGAFLDPWVLVCTELWSV
PPWPGWLWERVWSHAASAQTTVWEVHSTACG*S
VRACHPAHEDRVIKPFKSLWSVASI*FQVACGFQ
PWQW*PSAGPPGLSSHRPLPTGCLAPACFSS*P
GCVLCSSPDVSSLFSSLVPSSGMFFPVHLLSWA
SPGTPVRAE*G*ASPGPAFLCTGSTEHPGCTRGV
LATPRVLAAPPLWCVFGTVQAGVGTASHSVSGQ
PCWDLLGICIGVQDKVLSPLDVFTHCCSLRS*P
QKSTENNSGTELTDGHCLCSVSVYWA*RASIAEP
CCSLSRPHLSSPGHVAGIEAFRFSLGFRILHLY
VSVFTNKNIALKMQKPSTGSAAGSWAFDSAWLL
LMHLVLGTLHGGF*LPCVSSLESTFQSLPVSSGF
GDWR*PDLLSWVSCQPGRRVGGF*ASPF*QLPA
CFAEGLHPVLPPAPGGGFPSLPSLPSLPVLRVV
GIEMLQRAVCEVKWHRCHVRFSDDSAESGVFTTL
TSPLDSLPCELPVDVFFSFFFWLLVWVLFCWLV
GVPDIPDYGFSVVSLNCVSVAPDVQKLKPYT*S
ADLILYEWCYFRPTQNILTFPEAGNHCHVFV**L
*SFVFNILGFYIWGFVCM*QVLLGHCSLLKQID
*GHLFSLKTKWNCEMRGMDQKKILPQGQRWGRS
QHPTKDLNPFWKVGSGLVGGC*AEEG*GGSWPHR
EALAWSRV*KWIN*KTGQSSYLGGPLPEQPDGP
PGPHLPAVEMEVWRTWGRAEDRTPHAVRAASGS
RLQKGQRSALGTIP*ALAATPRGSHEADQGP*GT
KCSGAAPWAGLAQPPHGLLGK*QALCKPTEVPS
SLPNFGTFHHPAEKPSLSSHCSLPLSP*QPAIN
LPSVSIDLPTQDIHVNVAFAFTQQDIFMVPVCCS
MHWSFIPSNG*IISCMDAPHLIYPFIRCWTFGW
FLLFGYSENAAVNICEQVFMGT

Frame 1

RLGLPKCWDYRLVSNSWPEVILQPQPPK*LGLQ
VESLLADPSRLL*YVQKARAAHAVRPRTRSQSV
CLVQVTMVFSSVPPASCVGH*ELPSLSRRRFWAL
FAGVTALMAAASVTPPGSLELLQPGFSKTLLGT
KLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLA
SILSSGPQNCAACVHEGIYEEGISILESSSAFPD
NAARREVESLPAVCPSDGCTWKGTLKEYESCHE
GRCPLMLTECPACKGLVRLGEKERHLEHECPER
SLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCG
KKKIPREKFQDHVKTCGKCRVPCRFHAIGCLET
VEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLG
DQSHAGSELLQRCESLEKKTATFENIVCVLNREV
ERVAMTAEACSRQHRLDQDKIEALSSKVVLAPG
RCSSWRGALASRTWRWLTWSRRSWRWRHPPTMG
SSSGRSQTSPGSARKLWLAAYPPSSPQPSTPAGT
ATRCVCVST*TATAPGEEHTCPSSLW**RARMT
PCCGGPSTRR*P*CCSTRITGST*LTPSGPT*L
HPLFRGQSTT*TSQAAAPSSAPSPRWRQRIPTCG
TMPSSSRPLWT*QGSNCPLLVSGGWGQPGTAGS
RRGHHAGPGSHCTSGQGPRLGAWEGVGLQPSSL
SRGKEPPASPQISETAEGLGRRS*PRAVVALAEG
LRVLPSTSCPCCPVQ*RERPWVGDTQSGSTSQQ
CPCSRSTVDGLVSLGHDRQKRGLLQEKGLLLAR
ARKAEQLGSPLWPLERREHS*TPGCLSAQSSGLC
HLGQAGCGRGSGPTPPLLRPLCGRCTAQPAGKV
*ELAIQLTKTELLNHSNLCGQWHPFDSRLPAAS
SLGSGDPLRVLLASPLTDPSPRGA*PLPASLASQ
AVFSVPHLM*APSSRALFPLLECSSLSISSPGP
PQGLL*EQNEDEPALVLPFCAQAPPSILAAPGG
SWPHRGSWLHRPCGVFLGQCRQA*ARLHTRCQGS
RAGTYWASVSGSRIKCCPLSTSSRTAVV*EVDH
RKVQKITQARS*PTGIVCVP*VCTGPKEQALQS
PAVPFPGLTSAPQATWRELRRSGSLWALEFCICT
FLYSQIRILH*KCRNHPLGQLQVAGLLTQPGYF
*CILCWALYMVDSDCRV*VP*RAPSSLFPCLQG
LVTGDSQTCSRGSPVNLEGGLVVSRPPLFSSCQL
ALQRGCIRSFHQHQGEGSHHSHPSHPCPCFVLL
ELRCCSVLCVK*SGIAVTCVSLMTRRSQASLPR
LPLPWILSRANCLLMSFSHFSSGCWSGSYSAGW*
EFLIFLITDSRLCL*TAFLLLLMCRSLSLTHDQ
LILSFMNGAISDRLRTS*PSLRLETTVMFSSND
CEVLSLTYWASIFGDLFVCDRFCWVTAVC*NRLI
EVISSHLKRNGTVK*EEWTKKKSSPRDKDGEEA
NIPQRI*THFGRWEVGWWVAAEQKRAEGEAGHT
GRPWHGAESKSGSTERRGRAATLEVLCQSSRMVL
LALTSLQWRWRSGEPGEELRTAPPTQ*EQHRAP
GCRRGRGLHSEQFPRHWLPHQEAPTKQTKALEG
QNALEQPRGQAWPSRLMAFWGSDRLCANPQRYHR
HYLILGHFITLQRNLPLAVTVPSRSAPDSQP*T
YLLSL*ICLLRTFM*MWPLLLLSKIFSWFLCVA
ACIGVSFLLMAE*SLVWMHHTLFIHSSVAGHLGG
FYFLATLKMLL*TFVNKFLWGR

Frame 2

A*ASQSAGITGWSRTPGLK*SSNLSLPSSWDYR
LNRS*LTPHVSYDTYRKQEQPMPSGRAPGLSLC
VWFR*PWCSVQFLLPRV*ATESSLV*AGGGSGL
CSRGSQLSWLQLA*PPLAPWSCYSPASPRPSWGP
SWKPSTCAPPAETSSAGPSRRSVATGTAPSAWP
ASSALGLRTVLPVFTRAYMKKAFLF*KAVRPSQ
IMLPAGRWRACRPSVPVMDAPGRGP*KNTRAATK
AAARSC*PNVPRAKAWSALVKRSATWSTSARRE
A*AAGIAGHPAAEQT*RRTTRSAPSSP*LVTAA
ARRRSPGRSFRTTSRLVASVESLADSTPSAASRR
*RVRNSRSTRCSGCGSTWPCY*ARCWRQSPSWE
TRATRGQSSCRGARAWRRRRPLLRTLSAS*TGR
WRGWP*LPRPAAGSTGWTKTRLKP*VARWSWHPA
GAAAGEEHWPQGPGDG*LGAEGLGDGGIHLRWG
LHLEDLRLRQEAPGSCGWPHTRHLLPSLLHQQV
RLQDVSAYLPERRRHRARNTPVPLLCGDEGPE*R
PAAVALQPEGDLNAARPE*PGARD*RLQARRDF
ILFSEASQRHEHRKRLPPLLPRLQDGGKEFLRA
GRCHLHQGHCGPDRALTAPYWCLGVGGSQAQPAH
GGATTLGQGLTVQVGRGRAWALGRVSACSQVHC
HGGRSHQPVLRFQRLRRGLADGLSQGLWWHWPR
VFGCFPAQAALAVLCSEGRGPGWGTLRVGAHPSS
AHVAGAQWMALCPSGMTGRNEGCSRRRASCWPE
QGRLSSLVLPSGPWREGSIPRPLGACLHRALVC
ATLARLAVGEGLVPRRLCSDHCVGGAQHSLRVKC
ESLPSSSRRQSY*TIQISVVSGIHLIPGCLRLP
ALAVVTLCGSSWPLLSQTPPHGVPSPCLLL*LA
RLCSLFLT*CKLPLLEPCSLFWNVLPCPSPLLGL
PRDSCESRMRMSQPWSCLSVHRLHRASWLHPGG
PGHTEGPGCTAPVVCFWDSAGRRRHGFTLGVRA
AVLGPTGHLYRGPG*SAVPSRRLHALL*FEKLTT
EKYRK*LRHGADRRALSVFRECVLGLKSKHCRA
LLFPFPASPQLPRPRGGN*GVPVLFGL*NFASV
RFCIHK*EYCIKNAETIHWVSCR*LGF*LSLATF
NASCAGHSTWWILTAVCEFPREHLPVSSRVFRV
W*LEIARPALVGLLSTWKEGWWFLGLPFLAVAS
LLCRGAASGPSTSTRGRVPITPIPPIPARASCCW
N*DAAACCV*SEVASLSRAFL**LGGVRRLYHA
YLSLGFSPVRIAC*CLFLIFLLVVGLGLILLVG
RSS*YS*LRILGCVFELRFCCS*CAEA*ALHMIS
*SYPL*MVLFPTDSEHPDLP*GWKPLSCFRLMI
VKFCL*HTGLLYLGICLYVTGFAGSLQFVKTD*
LRSSLLT*NEMEL*NERNGPKKNPPPGTKMGKKP
TSHKGFEPILEGGKWVGGWLLSRRGLRGKLATP
GGPGMEQSLKVDQLKDGAEQLPWRSFARAAGWS
SWPSPPCSGDGGLENLGKS*GPHPPRSESSIGLQ
AAEGAEVCTRNNSLGTGCHTKRLPRSRPRPLRD
KMLWSSPVGRPGPAASWPSGEVTGSVQTHRGTI
VTT*FWDISSPCRETFP*QSLFPPAQPLTASHKP
TFCLYRFAYSGHSCECGLCFYSARYFHGSCVLQ
HALEFHSF*WLNNLLYGCTTPYLSIHPLLDIWV
VSTFWLL*KCCCEHL*TSFYGDV

Sequence TRAF2.aAug05

CGGTAGCTGGGCGGGCCCTTAGTTCCGGGCGCGCTGCGACCGTTGGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCC
TGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGC
AGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGG
GCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAG
TGATGGAT
GCACCTGGAAGGGGACCCTGAAAGAATACGAGAGCTGCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTG
GTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAAGCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGA
AGGCGCACCACGAGGTCTGCCCCAAGTTCCCCTTAACTTGTGACGGCTGCGGCAAGAAGAAGATCCCCCGGGAGAAGTTTCAGGACCACGTCAAGACTTG
TGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCTGCCTCGAGACGGTAGAGGGTGAGAAACAGCAGGAGCACGAGGTGCAGTGGCTGCGGGAG
CACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAGACCAGAGCCACGCGGGGTCAGAGCTCCTGCAGAGGTGCGAGAGCCTGG
AGAAGAAGACGGCCACTTTTGAGAACATTGTCTGCGTCCTGAACCGGGAGGTGGAGAGGGTGGCCATGACTGCCGAGGCCTGCAGCCGGCAGCACCGGCT
GGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTC
TTGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCAGGAAGCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCA
TCTTCTCCCCAGCCTTCTACACCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCACCGGGCGAGGAACACACCTGTCCCT
CTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGACCTTAATGCTGCTCGACCAGAATAACCGGGAGCAC
GTGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACATGAACATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCT
CCAAGATGGAGGCAAAGAATTCCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGCTCTAACTGCCCCCTACTGGTGTCTG
GGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGCCACCACGCTGGGCCAGGGTCTCACTGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTG
GGAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCTCAGATTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCT
TAGCCAAGGGCTGTGGTGGCATTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTGCAGTGAAGGGAGAGGCCCTGGGTGG
GGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGTAGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCT
GCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCTGGCCCCTGGAGAGAAGGGAGCATTCCTAGACCCCT
GGGTGCTTGTCTGCACAGAGCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTCTGGTCCCACGCCGCCTCTGCTCAGACCACTGTGTG
GGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTTGCCATCCAGCTCACGAAGACAGAGTTATTAAACCATTCAAATCTCTGTGGTCAGTGGCA
TCCATTTGATTCCAGGTTGCCTGCGGCTTCCAGCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCCTGGCCTCTCCTCTCACAGACCCCTCCCCACGGGGT
GCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCAGGCTGTGTTCTCTGTTCCTCACCTGATGTAAGCTCCCTCTTCTCGAGCCTTGTTCCCTCTTCTGGAAT
GTTCTTCCCTGTCCATCTCCTCTCCTGGGCCTCCCCAGGGACTCCTGTGAGAGCAGAATGAGGATGAGCCAGCCCTGGTCCTGCCTTTCTGTGCACAGGC
TCCACCGAGCATCCTGGCTGCACCCGGGGGGTCCTGGCCACACCGAGGGTCCTGGCTGCACCGCCCCTGTGGTGTGTTTTTGGGACAGTGCAGGCAGGCG
TAGGCACGGCTTCACACTCGGTGTCAGGGCAGCCGTGCTGGGACCTACTGGGCATCTGTATCGGGGTCCAGGATAAAGTGCTGTCCCCTCTCGACGTCTT
CACGCACTGCTGTAGTTTGAGAAGTTGACCACAGAAAAGTACAGAAAATAACTCAGGCACGGAGCTGACCGACGGGCATTGTCTGTGTTCCGTGAGTGTG
TACTGGGCCTAAAGAGCAAGCATTGCAGAGCCCTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTCCCCAGGCCACGTGGCGGGAATTGAGGCGTTCCGGT
TCTCTTTGGGCTTTAGAATTTTGCATCTGTACGTTTCTGTATTCACAAATAAGAATATTGCATTAAAAATGCAGAAACCATCCACTGGGTCAGCTGCAGG
TAGCTGGGCTTTTGACTCAGCCTGGCTACTTTTAATGCATCTTGTGCTGGGCACTCTACATGGTGGATTCTGACTGCCGTGTGTGAGTTCCCTAGAGAGC
ACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTTTGGTGACTGGAGATAGCCAGACCTGCTCTCGTGGGTCTCCTGTCAACCTGGAAGGAGGGTTGGTGGTT
TCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCTTGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCACCAGCACCAGGGGGAGGGTTCCCATCACTCCCATC
CCTCCCATCCCTGCCCGTGCTTCGTGTTGTTGGAATTGAGATGCTGCAGCGTGCTGTGTGTGAAGTGAAGTGGCATCGCTGTCACGTGCGTTTCTCTGAT
GACTCGGCGGAGTCAGGCGTCTTTACCACGCTTACCTCTCCCTTGGATTCTCTCCCGTGCGAATTGCCTGTTGATGTCTTTTTCTCATTTTTCTTCTGGT
TGTTGGTCTGGGTCTTATTCTGCTGGTTGGTAGGAGTTCCTGATATTCCTGATTACGGATTCTCGGTTGTGTCTTTGAACTGCGTTTCTGTTGCTCCTGA
TGTGCAGAAGCTTAAGCCTTACACATGATCAGCTGATCTTATCCTTTATGAATGGTGCTATTTCCGACCGACTCAGAACATCCTGACCTTCCCTGAGGCT
GGAAACCACTGTCATGTTTTCGTCTAATGATTGTGAAGTTTTGTCTTTAACATACTGGGCTTCTATATTTGGGGATTTGTTTGTATGTGACAGGTTTTGC
TGGGTCACTGCAGTTTGTTAAAACAGATTGATTGAGGTCATCTCTTCTCACTTAAAACGAAATGGAACTGTGAAATGAGAGGAATGGACCAAAAAAAAAT
CCTCCCCCAGGGACAAAGATGGGGAAGAAGCCAACATCCCACAAAGGATTTGAACCCATTTTGGAAGGTGGGAAGTGGGTTGGTGGGTGGCTGCTGAGCA
GAAGAGGGCTGAGGGGGAAGCTGGCCACACCGGGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTGGATCAACTGAAAGACGGGGCAGAGCAGCTACCTTG
GAGGTCCTTTGCCAGAGCAGCCGGATGGTCCTCCTGGCCCTCACCTCCCTGCAGTGGAGATGGAGGTCTGGAGAACCTGGGGAAGAGCTGAGGACCGCAC
CCCCCACGCAGTGAGAGCAGCATCGGGCTCCAGGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAACAATTCCCTAGGCACTGGCTGCCACACCAAGAGGC
TCCCACGAAGCAGACCAAGGCCCTTGAGGGACAAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCTGGCCCAGCCGCCTCATGGCCTTCTGGGGAAGTGAC
AGGCTCTGTGCAAACCCACAGAGGTACCATCGTCACTACCTAATTTTGGGACATTTCATCACCCTGCAGAGAAACCTTCCCTTAGCAGTCACTGTTCCCT
CCCGCTCAGCCCCTGACAGCCAGCCATAAACCTACCTTCTGTCTCTATAGATTTGCCTACTCAGGACATTCATGTGAATGTGGCCTTTGCTTTTACTCAG
CAAGATATTTTCATGGTTCCTGTGTGTTGCAGCATGCATTGGAGTTTCATTCCTTCTAATGGCTGAATAATCTCTTGTATGGATGCACCACACCTTATTT
ATCCATTCATCCGTTGCTGGACATTTGGGTGGTTTCTACTTTTTGGCTACTCTGAAAATGCTGCTGTGAACATTTGTGAACAAGTTTTTATGGGGACGTG
  • TRAF2-3 (forward primer)
  • TRAF2-4 (reverse primer)
  • Alternative donor

Frame 0

R*LGGPLVPGALRPLGLCSRGSQLSWLQLA*PP
LAPWSCYSPASPRPSWGPSWKPSTCAPPAETSSA
GPSRRSVATGTAPSAWPASSALGLRTVLPVFTR
AYMKKAFLF*KAVRPSQIMLPAGRWRACRPSVP
VMD
APGRGP*KNTRAATKAAARSC*PNVPRAKAW
SALVKRSATWSTSARREA*AAGIAGHPAAEQT*
RRTTRSAPSSP*LVTAAARRRSPGRSFRTTSRL
VASVESLADSTPSAASRR*RVRNSRSTRCSGCGS
TWPCY*ARCWRQSPSWETRATRGQSSCRGARAW
RRRRPLLRTLSAS*TGRWRGWP*LPRPAAGSTG
WTKTRLKP*VARCSSWRGALASRTWRWLTWSRRS
WRWRHPPTMGSSSGRSQTSPGSARKLWLAAYPP
SSPQPSTPAGTATRCVCVST*TATAPGEEHTCP
SSLW**RARMTPCCGGPSTRR*P*CCSTRITGST
*LTPSGPT*LHPLFRGQSTT*TSQAAAPSSAPS
PRWRQRIPTCGTMPSSSRPLWT*QGSNCPLLVS
GGWGQPGTAGSRRGHHAGPGSHCTSGQGPRLGAW
EGVGLQPSSLSRGKEPPASPQISETAEGLGRRS
*PRAVVALAEGLRVLPSTSCPCCPVQ*RERPWV
GDTQSGSTSQQCPCSRSTVDGLVSLGHDRQKRGL
LQEKGLLLARARKAEQLGSPLWPLERREHS*TP
GCLSAQSSGLCHLGQAGCGRGSGPTPPLLRPLC
GRCTAQPAGKV*ELAIQLTKTELLNHSNLCGQWH
PFDSRLPAASSLGSGDPLRVLLASPLTDPSPRG
A*PLPASLASQAVFSVPHLM*APSSRALFPLLE
CSSLSISSPGPPQGLL*EQNEDEPALVLPFCAQA
PPSILAAPGGSWPHRGSWLHRPCGVFLGQCRQA
*ARLHTRCQGSRAGTYWASVSGSRIKCCPLSTS
SRTAVV*EVDHRKVQKITQARS*PTGIVCVP*VC
TGPKEQALQSPAVPFPGLTSAPQATWRELRRSG
SLWALEFCICTFLYSQIRILH*KCRNHPLGQLQ
VAGLLTQPGYF*CILCWALYMVDSDCRV*VP*RA
PSSLFPCLQGLVTGDSQTCSRGSPVNLEGGLVV
SRPPLFSSCQLALQRGCIRSFHQHQGEGSHHSH
PSHPCPCFVLLELRCCSVLCVK*SGIAVTCVSLM
TRRSQASLPRLPLPWILSRANCLLMSFSHFSSG
CWSGSYSAGW*EFLIFLITDSRLCL*TAFLLLL
MCRSLSLTHDQLILSFMNGAISDRLRTS*PSLRL
ETTVMFSSNDCEVLSLTYWASIFGDLFVCDRFC
WVTAVC*NRLIEVISSHLKRNGTVK*EEWTKKK
SSPRDKDGEEANIPQRI*THFGRWEVGWWVAAEQ
KRAEGEAGHTGRPWHGAESKSGSTERRGRAATL
EVLCQSSRMVLLALTSLQWRWRSGEPGEELRTA
PPTQ*EQHRAPGCRRGRGLHSEQFPRHWLPHQEA
PTKQTKALEGQNALEQPRGQAWPSRLMAFWGSD
RLCANPQRYHRHYLILGHFITLQRNLPLAVTVP
SRSAPDSQP*TYLLSL*ICLLRTFM*MWPLLLLS
KIFSWFLCVAACIGVSFLLMAE*SLVWMHHTLF
IHSSVAGHLGGFYFLATLKMLL*TFVNKFLWGR

Frame 1

GSWAGP*FRARCDRWGFVRGGHSSHGCS*RDPP
WLPGVATARLLQDPPGDQAGSQVPVLRLQKRPP
QALPGAVWPPVLLLLPGQHPQLWASELCCLCSRG
HI*RRHFYFRKQFGLPR*CCPQGGGEPAGRLSQ
*WM
HLEGDPERIRELPRRPLPAHADRMSRVQRP
GPPW*KGAPPGARVPGEKPELPALPGTLLRSRRE
GAPRGLPQVPLNL*RLRQEEDPPGEVSGPRQDL
WQVSSPLQIPRHRLPRDGRG*ETAGARGAVAAG
APGHATELGAGGKAPLGRPEPRGVRAPAEVREPG
EEDGHF*EHCLRPEPGGGEGGHDCRGLQPAAPA
GPRQD*SPE*QGAAAGEEHWPQGPGDG*LGAEG
LGDGGIHLRWGLHLEDLRLRQEAPGSCGWPHTRH
LLPSLLHQQVRLQDVSAYLPERRRHRARNTPVP
LLCGDEGPE*RPAAVALQPEGDLNAARPE*PGA
RD*RLQARRDFILFSEASQRHEHRKRLPPLLPRL
QDGGKEFLRAGRCHLHQGHCGPDRALTAPYWCL
GVGGSQAQPAHGGATTLGQGLTVQVGRGRAWAL
GRVSACSQVHCHGGRSHQPVLRFQRLRRGLADGL
SQGLWWHWPRVFGCFPAQAALAVLCSEGRGPGW
GTLRVGAHPSSAHVAGAQWMALCPSGMTGRNEG
CSRRRASCWPEQGRLSSLVLPSGPWREGSIPRPL
GACLHRALVCATLARLAVGEGLVPRRLCSDHCV
GGAQHSLRVKCESLPSSSRRQSY*TIQISVVSG
IHLIPGCLRLPALAVVTLCGSSWPLLSQTPPHGV
PSPCLLL*LARLCSLFLT*CKLPLLEPCSLFWN
VLPCPSPLLGLPRDSCESRMRMSQPWSCLSVHR
LHRASWLHPGGPGHTEGPGCTAPVVCFWDSAGRR
RHGFTLGVRAAVLGPTGHLYRGPG*SAVPSRRL
HALL*FEKLTTEKYRK*LRHGADRRALSVFREC
VLGLKSKHCRALLFPFPASPQLPRPRGGN*GVPV
LFGL*NFASVRFCIHK*EYCIKNAETIHWVSCR
*LGF*LSLATFNASCAGHSTWWILTAVCEFPRE
HLPVSSRVFRVW*LEIARPALVGLLSTWKEGWWF
LGLPFLAVASLLCRGAASGPSTSTRGRVPITPI
PPIPARASCCWN*DAAACCV*SEVASLSRAFL*
*LGGVRRLYHAYLSLGFSPVRIAC*CLFLIFLLV
VGLGLILLVGRSS*YS*LRILGCVFELRFCCS*
CAEA*ALHMIS*SYPL*MVLFPTDSEHPDLP*G
WKPLSCFRLMIVKFCL*HTGLLYLGICLYVTGFA
GSLQFVKTD*LRSSLLT*NEMEL*NERNGPKKN
PPPGTKMGKKPTSHKGFEPILEGGKWVGGWLLS
RRGLRGKLATPGGPGMEQSLKVDQLKDGAEQLPW
RSFARAAGWSSWPSPPCSGDGGLENLGKS*GPH
PPRSESSIGLQAAEGAEVCTRNNSLGTGCHTKR
LPRSRPRPLRDKMLWSSPVGRPGPAASWPSGEVT
GSVQTHRGTIVTT*FWDISSPCRETFP*QSLFP
PAQPLTASHKPTFCLYRFAYSGHSCECGLCFYS
ARYFHGSCVLQHALEFHSF*WLNNLLYGCTTPYL
SIHPLLDIWVVSTFWLL*KCCCEHL*TSFYGDV

Frame 2

VAGRALSSGRAATVGALFAGVTALMAAASVTPP
GSLELLQPGFSKTLLGTKLEAKYLCSACRNVLR
RPFQAQCGHRYCSFCLASILSSGPQNCAACVHE
GIYEEGISILESSSAFPDNAARREVESLPAVCPS
DG
CTWKGTLKEYESCHEGRCPLMLTECPACKGL
VRLGEKERHLEHECPERSLSCRHCRAPCCGADV
KAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTC
GKCRVPCRFHAIGCLETVEGEKQQEHEVQWLRE
HLAMLLSSVLEAKPLLGDQSHAGSELLQRCESL
EKKTATFENIVCVLNREVERVAMTAEACSRQHRL
DQDKIEALSSKVQQLERSIGLKDLAMADLEQKV
LEMEASTYDGVFIWKISDFARKRQEAVAGRIPA
IFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSL
FFVVMKGPNDALLRWPFNQKVTLMLLDQNNREH
VIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPV
SKMEAKNSYVRDDAIFIKAIVDLTGL*LPPTGVW
GLGAARHSRLTEGPPRWARVSLYKWAGAALGRL
GGCRPAAKFTVTGEGATSQSSDFRDCGGAWQTV
LAKGCGGIGRGSSGASQHKLPLLSCAVKGEALGG
GHSEWEHIPAVPM*QEHSGWPCVPRA*QAETRA
APGEGPPAGQSKEG*AAWFSPLAPGEKGAFLDP
WVLVCTELWSVPPWPGWLWERVWSHAASAQTTVW
EVHSTACG*SVRACHPAHEDRVIKPFKSLWSVA
SI*FQVACGFQPWQW*PSAGPPGLSSHRPLPTG
CLAPACFSS*PGCVLCSSPDVSSLFSSLVPSSGM
FFPVHLLSWASPGTPVRAE*G*ASPGPAFLCTG
STEHPGCTRGVLATPRVLAAPPLWCVFGTVQAG
VGTASHSVSGQPCWDLLGICIGVQDKVLSPLDVF
THCCSLRS*PQKSTENNSGTELTDGHCLCSVSV
YWA*RASIAEPCCSLSRPHLSSPGHVAGIEAFR
FSLGFRILHLYVSVFTNKNIALKMQKPSTGSAAG
SWAFDSAWLLLMHLVLGTLHGGF*LPCVSSLES
TFQSLPVSSGFGDWR*PDLLSWVSCQPGRRVGG
F*ASPF*QLPACFAEGLHPVLPPAPGGGFPSLPS
LPSLPVLRVVGIEMLQRAVCEVKWHRCHVRFSD
DSAESGVFTTLTSPLDSLPCELPVDVFFSFFFW
LLVWVLFCWLVGVPDIPDYGFSVVSLNCVSVAPD
VQKLKPYT*SADLILYEWCYFRPTQNILTFPEA
GNHCHVFV**L*SFVFNILGFYIWGFVCM*QVL
LGHCSLLKQID*GHLFSLKTKWNCEMRGMDQKKI
LPQGQRWGRSQHPTKDLNPFWKVGSGLVGGC*A
EEG*GGSWPHREALAWSRV*KWIN*KTGQSSYL
GGPLPEQPDGPPGPHLPAVEMEVWRTWGRAEDRT
PHAVRAASGSRLQKGQRSALGTIP*ALAATPRG
SHEADQGP*GTKCSGAAPWAGLAQPPHGLLGK*
QALCKPTEVPSSLPNFGTFHHPAEKPSLSSHCSL
PLSP*QPAINLPSVSIDLPTQDIHVNVAFAFTQ
QDIFMVPVCCSMHWSFIPSNG*IISCMDAPHLI
YPFIRCWTFGWFLLFGYSENAAVNICEQVFMGT

Sequence TRAF2.bAug05

CGGTAGCTGGGCGGGCCCTTAGTTCCGGGCGCGCTGCGACCGTTGGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCC
TGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGC
AGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGG
GCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAG
TGATGGAT
GCACCTGGAAGGGGACCCTGAAAGAATACGAGAGCTGCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTG
GTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAAGCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGA
AGTTTCAGGACCACGTCAAGACTTGTGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCTGCCTCGAGACGGTAGAGGGTGAGAAACAGCAGGA
GCACGAGGTGCAGTGGCTGCGGGAGCACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAGACCAGAGCCACGCGGGGTCAGAG
CTCCTGCAGAGGTGCGAGAGCCTGGAGAAGAAGACGGCCACTTTTGAGAACATTGTCTGCGTCCTGAACCGGGAGGTGGAGAGGGTGGCCATGACTGCCG
AGGCCTGCAGCCGGCAGCACCGGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGC
GATGGCTGACTTGGAGCAGAAGGTCTTGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCAGGAAGCGCCAGGAA
GCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACACCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCA
CCGGGCGAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGACCTTAATGCT
GCTCGACCAGAATAACCGGGAGCACGTGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACATGAACATCGCAAGC
GGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATTCCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGC
TCTAACTGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGCCACCACGCTGGGCCAGGGTCTCACTGTACAAGTG
GGCAGGGGCCGCGCTTGGGCGCTTGGGAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCTCAGATTTCAGAGAC
TGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCATTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTG
CAGTGAAGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGTAGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCG
GGCATGACAGGCAGAAACGAGGGCTGCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCTGGCCCCTGGA
GAGAAGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGAGCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTCTGGTCCCACG
CCGCCTCTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTTGCCATCCAGCTCACGAAGACAGAGTTATTAAACC
ATTCAAATCTCTGTGGTCAGTGGCATCCATTTGATTCCAGGTTGCCTGCGGCTTCCAGCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCCTGGCCTCTCC
TCTCACAGACCCCTCCCCACGGGGTGCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCAGGCTGTGTTCTCTGTTCCTCACCTGATGTAAGCTCCCTCTTCT
CGAGCCTTGTTCCCTCTTCTGGAATGTTCTTCCCTGTCCATCTCCTCTCCTGGGCCTCCCCAGGGACTCCTGTGAGAGCAGAATGAGGATGAGCCAGCCC
TGGTCCTGCCTTTCTGTGCACAGGCTCCACCGAGCATCCTGGCTGCACCCGGGGGGTCCTGGCCACACCGAGGGTCCTGGCTGCACCGCCCCTGTGGTGT
GTTTTTGGGACAGTGCAGGCAGGCGTAGGCACGGCTTCACACTCGGTGTCAGGGCAGCCGTGCTGGGACCTACTGGGCATCTGTATCGGGGTCCAGGATA
AAGTGCTGTCCCCTCTCGACGTCTTCACGCACTGCTGTAGTTTGAGAAGTTGACCACAGAAAAGTACAGAAAATAACTCAGGCACGGAGCTGACCGACGG
GCATTGTCTGTGTTCCGTGAGTGTGTACTGGGCCTAAAGAGCAAGCATTGCAGAGCCCTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTCCCCAGGCCAC
GTGGCGGGAATTGAGGCGTTCCGGTTCTCTTTGGGCTTTAGAATTTTGCATCTGTACGTTTCTGTATTCACAAATAAGAATATTGCATTAAAAATGCAGA
AACCATCCACTGGGTCAGCTGCAGGTAGCTGGGCTTTTGACTCAGCCTGGCTACTTTTAATGCATCTTGTGCTGGGCACTCTACATGGTGGATTCTGACT
GCCGTGTGTGAGTTCCCTAGAGAGCACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTTTGGTGACTGGAGATAGCCAGACCTGCTCTCGTGGGTCTCCTGT
CAACCTGGAAGGAGGGTTGGTGGTTTCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCTTGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCACCAGCACCAG
GGGGAGGGTTCCCATCACTCCCATCCCTCCCATCCCTGCCCGTGCTTCGTGTTGTTGGAATTGAGATGCTGCAGCGTGCTGTGTGTGAAGTGAAGTGGCA
TCGCTGTCACGTGCGTTTCTCTGATGACTCGGCGGAGTCAGGCGTCTTTACCACGCTTACCTCTCCCTTGGATTCTCTCCCGTGCGAATTGCCTGTTGAT
GTCTTTTTCTCATTTTTCTTCTGGTTGTTGGTCTGGGTCTTATTCTGCTGGTTGGTAGGAGTTCCTGATATTCCTGATTACGGATTCTCGGTTGTGTCTT
TGAACTGCGTTTCTGTTGCTCCTGATGTGCAGAAGCTTAAGCCTTACACATGATCAGCTGATCTTATCCTTTATGAATGGTGCTATTTCCGACCGACTCA
GAACATCCTGACCTTCCCTGAGGCTGGAAACCACTGTCATGTTTTCGTCTAATGATTGTGAAGTTTTGTCTTTAACATACTGGGCTTCTATATTTGGGGA
TTTGTTTGTATGTGACAGGTTTTGCTGGGTCACTGCAGTTTGTTAAAACAGATTGATTGAGGTCATCTCTTCTCACTTAAAACGAAATGGAACTGTGAAA
TGAGAGGAATGGACCAAAAAAAAATCCTCCCCCAGGGACAAAGATGGGGAAGAAGCCAACATCCCACAAAGGATTTGAACCCATTTTGGAAGGTGGGAAG
TGGGTTGGTGGGTGGCTGCTGAGCAGAAGAGGGCTGAGGGGGAAGCTGGCCACACCGGGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTGGATCAACTGA
AAGACGGGGCAGAGCAGCTACCTTGGAGGTCCTTTGCCAGAGCAGCCGGATGGTCCTCCTGGCCCTCACCTCCCTGCAGTGGAGATGGAGGTCTGGAGAA
CCTGGGGAAGAGCTGAGGACCGCACCCCCCACGCAGTGAGAGCAGCATCGGGCTCCAGGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAACAATTCCCTA
GGCACTGGCTGCCACACCAAGAGGCTCCCACGAAGCAGACCAAGGCCCTTGAGGGACAAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCTGGCCCAGCCG
CCTCATGGCCTTCTGGGGAAGTGACAGGCTCTGTGCAAACCCACAGAGGTACCATCGTCACTACCTAATTTTGGGACATTTCATCACCCTGCAGAGAAAC
CTTCCCTTAGCAGTCACTGTTCCCTCCCGCTCAGCCCCTGACAGCCAGCCATAAACCTACCTTCTGTCTCTATAGATTTGCCTACTCAGGACATTCATGT
GAATGTGGCCTTTGCTTTTACTCAGCAAGATATTTTCATGGTTCCTGTGTGTTGCAGCATGCATTGGAGTTTCATTCCTTCTAATGGCTGAATAATCTCT
TGTATGGATGCACCACACCTTATTTATCCATTCATCCGTTGCTGGACATTTGGGTGGTTTCTACTTTTTGGCTACTCTGAAAATGCTGCTGTGAACATTT
GTGAACAAGTTTTTATGGGGACGTG
  • TRAF2-3 (forward primer)
  • TRAF2-4 (reverse primer)
  • Alternative donor

Frame 0

R*LGGPLVPGALRPLGLCSRGSQLSWLQLA*PP
LAPWSCYSPASPRPSWGPSWKPSTCAPPAETSSA
GPSRRSVATGTAPSAWPASSALGLRTVLPVFTR
AYMKKAFLF*KAVRPSQIMLPAGRWRACRPSVP
VMD
APGRGP*KNTRAATKAAARSC*PNVPRAKAW
SALVKRSATWSTSARREA*AAGIAGHPAAEQT*
SFRTTSRLVASVESLADSTPSAASRR*RVRNSR
STRCSGCGSTWPCY*ARCWRQSPSWETRATRGQS
SCRGARAWRRRRPLLRTLSAS*TGRWRGWP*LP
RPAAGSTGWTKTRLKP*VARCSSWRGALASRTW
RWLTWSRRSWRWRHPPTMGSSSGRSQTSPGSARK
LWLAAYPPSSPQPSTPAGTATRCVCVST*TATA
PGEEHTCPSSLW**RARMTPCCGGPSTRR*P*C
CSTRITGST*LTPSGPT*LHPLFRGQSTT*TSQA
AAPSSAPSPRWRQRIPTCGTMPSSSRPLWT*QG
SNCPLLVSGGWGQPGTAGSRRGHHAGPGSHCTS
GQGPRLGAWEGVGLQPSSLSRGKEPPASPQISET
AEGLGRRS*PRAVVALAEGLRVLPSTSCPCCPV
Q*RERPWVGDTQSGSTSQQCPCSRSTVDGLVSL
GHDRQKRGLLQEKGLLLARARKAEQLGSPLWPLE
RREHS*TPGCLSAQSSGLCHLGQAGCGRGSGPT
PPLLRPLCGRCTAQPAGKV*ELAIQLTKTELLN
HSNLCGQWHPFDSRLPAASSLGSGDPLRVLLASP
LTDPSPRGA*PLPASLASQAVFSVPHLM*APSS
RALFPLLECSSLSISSPGPPQGLL*EQNEDEPA
LVLPFCAQAPPSILAAPGGSWPHRGSWLHRPCGV
FLGQCRQA*ARLHTRCQGSRAGTYWASVSGSRI
KCCPLSTSSRTAVV*EVDHRKVQKITQARS*PT
GIVCVP*VCTGPKEQALQSPAVPFPGLTSAPQAT
WRELRRSGSLWALEFCICTFLYSQIRILH*KCR
NHPLGQLQVAGLLTQPGYF*CILCWALYMVDSD
CRV*VP*RAPSSLFPCLQGLVTGDSQTCSRGSPV
NLEGGLVVSRPPLFSSCQLALQRGCIRSFHQHQ
GEGSHHSHPSHPCPCFVLLELRCCSVLCVK*SG
IAVTCVSLMTRRSQASLPRLPLPWILSRANCLLM
SFSHFSSGCWSGSYSAGW*EFLIFLITDSRLCL
*TAFLLLLMCRSLSLTHDQLILSFMNGAISDRL
RTS*PSLRLETTVMFSSNDCEVLSLTYWASIFGD
LFVCDRFCWVTAVC*NRLIEVISSHLKRNGTVK
*EEWTKKKSSPRDKDGEEANIPQRI*THFGRWE
VGWWVAAEQKRAEGEAGHTGRPWHGAESKSGSTE
RRGRAATLEVLCQSSRMVLLALTSLQWRWRSGE
PGEELRTAPPTQ*EQHRAPGCRRGRGLHSEQFP
RHWLPHQEAPTKQTKALEGQNALEQPRGQAWPSR
LMAFWGSDRLCANPQRYHRHYLILGHFITLQRN
LPLAVTVPSRSAPDSQP*TYLLSL*ICLLRTFM
*MWPLLLLSKIFSWFLCVAACIGVSFLLMAE*SL
VWMHHTLFIHSSVAGHLGGFYFLATLKMLL*TF
VNKFLWGR

Frame 1

GSWAGP*FRARCDRWGFVRGGHSSHGCS*RDPP
WLPGVATARLLQDPPGDQAGSQVPVLRLQKRPP
QALPGAVWPPVLLLLPGQHPQLWASELCCLCSRG
HI*RRHFYFRKQFGLPR*CCPQGGGEPAGRLSQ
*WM
HLEGDPERIRELPRRPLPAHADRMSRVQRP
GPPW*KGAPPGARVPGEKPELPALPGTLLRSRRE
VSGPRQDLWQVSSPLQIPRHRLPRDGRG*ETAG
ARGAVAAGAPGHATELGAGGKAPLGRPEPRGVR
APAEVREPGEEDGHF*EHCLRPEPGGGEGGHDCR
GLQPAAPAGPRQD*SPE*QGAAAGEEHWPQGPG
DG*LGAEGLGDGGIHLRWGLHLEDLRLRQEAPG
SCGWPHTRHLLPSLLHQQVRLQDVSAYLPERRRH
RARNTPVPLLCGDEGPE*RPAAVALQPEGDLNA
ARPE*PGARD*RLQARRDFILFSEASQRHEHRK
RLPPLLPRLQDGGKEFLRAGRCHLHQGHCGPDRA
LTAPYWCLGVGGSQAQPAHGGATTLGQGLTVQV
GRGRAWALGRVSACSQVHCHGGRSHQPVLRFQR
LRRGLADGLSQGLWWHWPRVFGCFPAQAALAVLC
SEGRGPGWGTLRVGAHPSSAHVAGAQWMALCPS
GMTGRNEGCSRRRASCWPEQGRLSSLVLPSGPW
REGSIPRPLGACLHRALVCATLARLAVGEGLVPR
RLCSDHCVGGAQHSLRVKCESLPSSSRRQSY*T
IQISVVSGIHLIPGCLRLPALAVVTLCGSSWPL
LSQTPPHGVPSPCLLL*LARLCSLFLT*CKLPLL
EPCSLFWNVLPCPSPLLGLPRDSCESRMRMSQP
WSCLSVHRLHRASWLHPGGPGHTEGPGCTAPVV
CFWDSAGRRRHGFTLGVRAAVLGPTGHLYRGPG*
SAVPSRRLHALL*FEKLTTEKYRK*LRHGADRR
ALSVFRECVLGLKSKHCRALLFPFPASPQLPRP
RGGN*GVPVLFGL*NFASVRFCIHK*EYCIKNAE
TIHWVSCR*LGF*LSLATFNASCAGHSTWWILT
AVCEFPREHLPVSSRVFRVW*LEIARPALVGLL
STWKEGWWFLGLPFLAVASLLCRGAASGPSTSTR
GRVPITPIPPIPARASCCWN*DAAACCV*SEVA
SLSRAFL**LGGVRRLYHAYLSLGFSPVRIAC*
CLFLIFLLVVGLGLILLVGRSS*YS*LRILGCVF
ELRFCCS*CAEA*ALHMIS*SYPL*MVLFPTDS
EHPDLP*GWKPLSCFRLMIVKFCL*HTGLLYLG
ICLYVTGFAGSLQFVKTD*LRSSLLT*NEMEL*N
ERNGPKKNPPPGTKMGKKPTSHKGFEPILEGGK
WVGGWLLSRRGLRGKLATPGGPGMEQSLKVDQL
KDGAEQLPWRSFARAAGWSSWPSPPCSGDGGLEN
LGKS*GPHPPRSESSIGLQAAEGAEVCTRNNSL
GTGCHTKRLPRSRPRPLRDKMLWSSPVGRPGPA
ASWPSGEVTGSVQTHRGTIVTT*FWDISSPCRET
FP*QSLFPPAQPLTASHKPTFCLYRFAYSGHSC
ECGLCFYSARYFHGSCVLQHALEFHSF*WLNNL
LYGCTTPYLSIHPLLDIWVVSTFWLL*KCCCEHL
*TSFYGDV

Frame 2

VAGRALSSGRAATVGALFAGVTALMAAASVTPP
GSLELLQPGFSKTLLGTKLEAKYLCSACRNVLR
RPFQAQCGHRYCSFCLASILSSGPQNCAACVHE
GIYEEGISILESSSAFPDNAARREVESLPAVCPS
DG
CTWKGTLKEYESCHEGRCPLMLTECPACKGL
VRLGEKERHLEHECPERSLSCRHCRAPCCGADV
KFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQE
HEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSE
LLQRCESLEKKTATFENIVCVLNREVERVAMTA
EACSRQHRLDQDKIEALSSKVQQLERSIGLKDLA
MADLEQKVLEMEASTYDGVFIWKISDFARKRQE
AVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDG
TGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLML
LDQNNREHVIDAFRPDVTSSSFQRPVNDMNIAS
GCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTG
L*LPPTGVWGLGAARHSRLTEGPPRWARVSLYKW
AGAALGRLGGCRPAAKFTVTGEGATSQSSDFRD
CGGAWQTVLAKGCGGIGRGSSGASQHKLPLLSC
AVKGEALGGGHSEWEHIPAVPM*QEHSGWPCVPR
A*QAETRAAPGEGPPAGQSKEG*AAWFSPLAPG
EKGAFLDPWVLVCTELWSVPPWPGWLWERVWSH
AASAQTTVWEVHSTACG*SVRACHPAHEDRVIKP
FKSLWSVASI*FQVACGFQPWQW*PSAGPPGLS
SHRPLPTGCLAPACFSS*PGCVLCSSPDVSSLF
SSLVPSSGMFFPVHLLSWASPGTPVRAE*G*ASP
GPAFLCTGSTEHPGCTRGVLATPRVLAAPPLWC
VFGTVQAGVGTASHSVSGQPCWDLLGICIGVQD
KVLSPLDVFTHCCSLRS*PQKSTENNSGTELTDG
HCLCSVSVYWA*RASIAEPCCSLSRPHLSSPGH
VAGIEAFRFSLGFRILHLYVSVFTNKNIALKMQ
KPSTGSAAGSWAFDSAWLLLMHLVLGTLHGGF*L
PCVSSLESTFQSLPVSSGFGDWR*PDLLSWVSC
QPGRRVGGF*ASPF*QLPACFAEGLHPVLPPAP
GGGFPSLPSLPSLPVLRVVGIEMLQRAVCEVKWH
RCHVRFSDDSAESGVFTTLTSPLDSLPCELPVD
VFFSFFFWLLVWVLFCWLVGVPDIPDYGFSVVS
LNCVSVAPDVQKLKPYT*SADLILYEWCYFRPTQ
NILTFPEAGNHCHVFV**L*SFVFNILGFYIWG
FVCM*QVLLGHCSLLKQID*GHLFSLKTKWNCE
MRGMDQKKILPQGQRWGRSQHPTKDLNPFWKVGS
GLVGGC*AEEG*GGSWPHREALAWSRV*KWIN*
KTGQSSYLGGPLPEQPDGPPGPHLPAVEMEVWR
TWGRAEDRTPHAVRAASGSRLQKGQRSALGTIP*
ALAATPRGSHEADQGP*GTKCSGAAPWAGLAQP
PHGLLGK*QALCKPTEVPSSLPNFGTFHHPAEK
PSLSSHCSLPLSP*QPAINLPSVSIDLPTQDIHV
NVAFAFTQQDIFMVPVCCSMHWSFIPSNG*IIS
CMDAPHLIYPFIRCWTFGWFLLFGYSENAAVNI
CEQVFMGT

Sequence TRAF2.cAug05

CGGTAGCTGGGCGGGCCCTTAGTTCCGGGCGCGCTGCGACCGTTGGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCC
TGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGC
AGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGG
GCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAG
TGATGGAT
GCACCTGGAAGGGGACCCTGAAAGAATACGAGGTAAAGATGCCTGCGTGTGGCATGGTGACAGAAGCTCCAGCAGTCGGGAGTCGTCCACGC
TCCCCAAGCAGTTATGACCTTGTCCTGCACGTTCCTCTCACTGGGGCTGAAGCCTGTCTGATGTCTGTGGAGGAAGAGACGGAGCTGCTCCTTAGA
AGCT
GCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTGGTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCC
GGAGAGAAGCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGAAGGCGCACCACGAGGTCTGCCCCAAGTTCCCCTTAACTTGTGAC
GGCTGCGGCAAGAAGAAGATCCCCCGGGAGAAGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCA
AGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTCTTGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCAGGAA
GCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACACCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAAC
GGCGACGGCACCGGGCGAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGA
CCTTAATGCTGCTCGACCAGAATAACCGGGAGCACGTGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACATGAA
CATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATTCCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGAC
CTGACAGGGCTCTAACTGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGCCACCACGCTGGGCCAGGGTCTCAC
TGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTGGGAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCTCAGA
TTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCATTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTG
CTGTCCTGTGCAGTGAAGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGTAGCAGGAGCACAGTGGATGGCCTT
GTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCTGCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCT
GGCCCCTGGAGAGAAGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGAGCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTC
TGGTCCCACGCCGCCTCTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTTGCCATCCAGCTCACGAAGACAGAG
TTATTAAACCATTCAAATCTCTGTGGTCAGTGGCATCCATTTGATTCCAGGTTGCCTGCGGCTTCCAGCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCC
TGGCCTCTCCTCTCACAGACCCCTCCCCACGGGGTGCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCAGGCTGTGTTCTCTGTTCCTCACCTGATGTAAGC
TCCCTCTTCTCGAGCCTTGTTCCCTCTTCTGGAATGTTCTTCCCTGTCCATCTCCTCTCCTGGGCCTCCCCAGGGACTCCTGTGAGAGCAGAATGAGGAT
GAGCCAGCCCTGGTCCTGCCTTTCTGTGCACAGGCTCCACCGAGCATCCTGGCTGCACCCGGGGGGTCCTGGCCACACCGAGGGTCCTGGCTGCACCGCC
CCTGTGGTGTGTTTTTGGGACAGTGCAGGCAGGCGTAGGCACGGCTTCACACTCGGTGTCAGGGCAGCCGTGCTGGGACCTACTGGGCATCTGTATCGGG
GTCCAGGATAAAGTGCTGTCCCCTCTCGACGTCTTCACGCACTGCTGTAGTTTGAGAAGTTGACCACAGAAAAGTACAGAAAATAACTCAGGCACGGAGC
TGACCGACGGGCATTGTCTGTGTTCCGTGAGTGTGTACTGGGCCTAAAGAGCAAGCATTGCAGAGCCCTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTC
CCCAGGCCACGTGGCGGGAATTGAGGCGTTCCGGTTCTCTTTGGGCTTTAGAATTTTGCATCTGTACGTTTCTGTATTCACAAATAAGAATATTGCATTA
AAAATGCAGAAACCATCCACTGGGTCAGCTGCAGGTAGCTGGGCTTTTGACTCAGCCTGGCTACTTTTAATGCATCTTGTGCTGGGCACTCTACATGGTG
GATTCTGACTGCCGTGTGTGAGTTCCCTAGAGAGCACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTTTGGTGACTGGAGATAGCCAGACCTGCTCTCGTG
GGTCTCCTGTCAACCTGGAAGGAGGGTTGGTGGTTTCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCTTGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCA
CCAGCACCAGGGGGAGGGTTCCCATCACTCCCATCCCTCCCATCCCTGCCCGTGCTTCGTGTTGTTGGAATTGAGATGCTGCAGCGTGCTGTGTGTGAAG
TGAAGTGGCATCGCTGTCACGTGCGTTTCTCTGATGACTCGGCGGAGTCAGGCGTCTTTACCACGCTTACCTCTCCCTTGGATTCTCTCCCGTGCGAATT
GCCTGTTGATGTCTTTTTCTCATTTTTCTTCTGGTTGTTGGTCTGGGTCTTATTCTGCTGGTTGGTAGGAGTTCCTGATATTCCTGATTACGGATTCTCG
GTTGTGTCTTTGAACTGCGTTTCTGTTGCTCCTGATGTGCAGAAGCTTAAGCCTTACACATGATCAGCTGATCTTATCCTTTATGAATGGTGCTATTTCC
GACCGACTCAGAACATCCTGACCTTCCCTGAGGCTGGAAACCACTGTCATGTTTTCGTCTAATGATTGTGAAGTTTTGTCTTTAACATACTGGGCTTCTA
TATTTGGGGATTTGTTTGTATGTGACAGGTTTTGCTGGGTCACTGCAGTTTGTTAAAACAGATTGATTGAGGTCATCTCTTCTCACTTAAAACGAAATGG
AACTGTGAAATGAGAGGAATGGACCAAAAAAAAATCCTCCCCCAGGGACAAAGATGGGGAAGAAGCCAACATCCCACAAAGGATTTGAACCCATTTTGGA
AGGTGGGAAGTGGGTTGGTGGGTGGCTGCTGAGCAGAAGAGGGCTGAGGGGGAAGCTGGCCACACCGGGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTG
GATCAACTGAAAGACGGGGCAGAGCAGCTACCTTGGAGGTCCTTTGCCAGAGCAGCCGGATGGTCCTCCTGGCCCTCACCTCCCTGCAGTGGAGATGGAG
GTCTGGAGAACCTGGGGAAGAGCTGAGGACCGCACCCCCCACGCAGTGAGAGCAGCATCGGGCTCCAGGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAA
CAATTCCCTAGGCACTGGCTGCCACACCAAGAGGCTCCCACGAAGCAGACCAAGGCCCTTGAGGGACAAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCT
GGCCCAGCCGCCTCATGGCCTTCTGGGGAAGTGACAGGCTCTGTGCAAACCCACAGAGGTACCATCGTCACTACCTAATTTTGGGACATTTCATCACCCT
GCAGAGAAACCTTCCCTTAGCAGTCACTGTTCCCTCCCGCTCAGCCCCTGACAGCCAGCCATAAACCTACCTTCTGTCTCTATAGATTTGCCTACTCAGG
ACATTCATGTGAATGTGGCCTTTGCTTTTACTCAGCAAGATATTTTCATGGTTCCTGTGTGTTGCAGCATGCATTGGAGTTTCATTCCTTCTAATGGCTG
AATAATCTCTTGTATGGATGCACCACACCTTATTTATCCATTCATCCGTTGCTGGACATTTGGGTGGTTTCTACTTTTTGGCTACTCTGAAAATGCTGCT
GTGAACATTTGTGAACAAGTTTTTATGGGGACGTG
  • TRAF2-3 (forward primer)
  • TRAF2-4 (reverse primer)
  • Alternative donor

Frame 0

R*LGGPLVPGALRPLGLCSRGSQLSWLQLA*PP
LAPWSCYSPASPRPSWGPSWKPSTCAPPAETSSA
GPSRRSVATGTAPSAWPASSALGLRTVLPVFTR
AYMKKAFLF*KAVRPSQIMLPAGRWRACRPSVP
VMD
APGRGP*KNTR*RCLRVAW*QKLQQSGVVHA
PQAVMTLSCTFLSLGLKPV*CLWRKRRSCSLE
A
ATKAAARSC*PNVPRAKAWSALVKRSATWSTSA
RREA*AAGIAGHPAAEQT*RRTTRSAPSSP*LVT
AAARRRSPGRSWTKTRLKP*VARCSSWRGALAS
RTWRWLTWSRRSWRWRHPPTMGSSSGRSQTSPG
SARKLWLAAYPPSSPQPSTPAGTATRCVCVST*T
ATAPGEEHTCPSSLW**RARMTPCCGGPSTRR*
P*CCSTRITGST*LTPSGPT*LHPLFRGQSTT*
TSQAAAPSSAPSPRWRQRIPTCGTMPSSSRPLWT
*QGSNCPLLVSGGWGQPGTAGSRRGHHAGPGSH
CTSGQGPRLGAWEGVGLQPSSLSRGKEPPASPQ
ISETAEGLGRRS*PRAVVALAEGLRVLPSTSCPC
CPVQ*RERPWVGDTQSGSTSQQCPCSRSTVDGL
VSLGHDRQKRGLLQEKGLLLARARKAEQLGSPL
WPLERREHS*TPGCLSAQSSGLCHLGQAGCGRGS
GPTPPLLRPLCGRCTAQPAGKV*ELAIQLTKTE
LLNHSNLCGQWHPFDSRLPAASSLGSGDPLRVL
LASPLTDPSPRGA*PLPASLASQAVFSVPHLM*A
PSSRALFPLLECSSLSISSPGPPQGLL*EQNED
EPALVLPFCAQAPPSILAAPGGSWPHRGSWLHR
PCGVFLGQCRQA*ARLHTRCQGSRAGTYWASVSG
SRIKCCPLSTSSRTAVV*EVDHRKVQKITQARS
*PTGIVCVP*VCTGPKEQALQSPAVPFPGLTSA
PQATWRELRRSGSLWALEFCICTFLYSQIRILH*
KCRNHPLGQLQVAGLLTQPGYF*CILCWALYMV
DSDCRV*VP*RAPSSLFPCLQGLVTGDSQTCSR
GSPVNLEGGLVVSRPPLFSSCQLALQRGCIRSFH
QHQGEGSHHSHPSHPCPCFVLLELRCCSVLCVK
*SGIAVTCVSLMTRRSQASLPRLPLPWILSRAN
CLLMSFSHFSSGCWSGSYSAGW*EFLIFLITDSR
LCL*TAFLLLLMCRSLSLTHDQLILSFMNGAIS
DRLRTS*PSLRLETTVMFSSNDCEVLSLTYWAS
IFGDLFVCDRFCWVTAVC*NRLIEVISSHLKRNG
TVK*EEWTKKKSSPRDKDGEEANIPQRI*THFG
RWEVGWWVAAEQKRAEGEAGHTGRPWHGAESKS
GSTERRGRAATLEVLCQSSRMVLLALTSLQWRWR
SGEPGEELRTAPPTQ*EQHRAPGCRRGRGLHSE
QFPRHWLPHQEAPTKQTKALEGQNALEQPRGQA
WPSRLMAFWGSDRLCANPQRYHRHYLILGHFITL
QRNLPLAVTVPSRSAPDSQP*TYLLSL*ICLLR
TFM*MWPLLLLSKIFSWFLCVAACIGVSFLLMA
E*SLVWMHHTLFIHSSVAGHLGGFYFLATLKMLL
*TFVNKFLWGR

Frame 1

GSWAGP*FRARCDRWGFVRGGHSSHGCS*RDPP
WLPGVATARLLQDPPGDQAGSQVPVLRLQKRPP
QALPGAVWPPVLLLLPGQHPQLWASELCCLCSRG
HI*RRHFYFRKQFGLPR*CCPQGGGEPAGRLSQ
*WM
HLEGDPERIRGKDACVWHGDRSSSSRESST
LPKQL*PCPARSSHWG*SLSDVCGGRDGAAP*
KL
PRRPLPAHADRMSRVQRPGPPW*KGAPPGARVP
GEKPELPALPGTLLRSRREGAPRGLPQVPLNL*
RLRQEEDPPGEAGPRQD*SPE*QGAAAGEEHWPQ
GPGDG*LGAEGLGDGGIHLRWGLHLEDLRLRQE
APGSCGWPHTRHLLPSLLHQQVRLQDVSAYLPE
RRRHRARNTPVPLLCGDEGPE*RPAAVALQPEGD
LNAARPE*PGARD*RLQARRDFILFSEASQRHE
HRKRLPPLLPRLQDGGKEFLRAGRCHLHQGHCG
PDRALTAPYWCLGVGGSQAQPAHGGATTLGQGLT
VQVGRGRAWALGRVSACSQVHCHGGRSHQPVLR
FQRLRRGLADGLSQGLWWHWPRVFGCFPAQAAL
AVLCSEGRGPGWGTLRVGAHPSSAHVAGAQWMAL
CPSGMTGRNEGCSRRRASCWPEQGRLSSLVLPS
GPWREGSIPRPLGACLHRALVCATLARLAVGEG
LVPRRLCSDHCVGGAQHSLRVKCESLPSSSRRQS
Y*TIQISVVSGIHLIPGCLRLPALAVVTLCGSS
WPLLSQTPPHGVPSPCLLL*LARLCSLFLT*CK
LPLLEPCSLFWNVLPCPSPLLGLPRDSCESRMRM
SQPWSCLSVHRLHRASWLHPGGPGHTEGPGCTA
PVVCFWDSAGRRRHGFTLGVRAAVLGPTGHLYR
GPG*SAVPSRRLHALL*FEKLTTEKYRK*LRHGA
DRRALSVFRECVLGLKSKHCRALLFPFPASPQL
PRPRGGN*GVPVLFGL*NFASVRFCIHK*EYCI
KNAETIHWVSCR*LGF*LSLATFNASCAGHSTWW
ILTAVCEFPREHLPVSSRVFRVW*LEIARPALV
GLLSTWKEGWWFLGLPFLAVASLLCRGAASGPS
TSTRGRVPITPIPPIPARASCCWN*DAAACCV*S
EVASLSRAFL**LGGVRRLYHAYLSLGFSPVRI
AC*CLFLIFLLVVGLGLILLVGRSS*YS*LRIL
GCVFELRFCCS*CAEA*ALHMIS*SYPL*MVLFP
TDSEHPDLP*GWKPLSCFRLMIVKFCL*HTGLL
YLGICLYVTGFAGSLQFVKTD*LRSSLLT*NEM
EL*NERNGPKKNPPPGTKMGKKPTSHKGFEPILE
GGKWVGGWLLSRRGLRGKLATPGGPGMEQSLKV
DQLKDGAEQLPWRSFARAAGWSSWPSPPCSGDG
GLENLGKS*GPHPPRSESSIGLQAAEGAEVCTRN
NSLGTGCHTKRLPRSRPRPLRDKMLWSSPVGRP
GPAASWPSGEVTGSVQTHRGTIVTT*FWDISSP
CRETFP*QSLFPPAQPLTASHKPTFCLYRFAYSG
HSCECGLCFYSARYFHGSCVLQHALEFHSF*WL
NNLLYGCTTPYLSIHPLLDIWVVSTFWLL*KCC
CEHL*TSFYGDV

Frame 2

VAGRALSSGRAATVGALFAGVTALMAAASVTPP
GSLELLQPGFSKTLLGTKLEAKYLCSACRNVLR
RPFQAQCGHRYCSFCLASILSSGPQNCAACVHE
GIYEEGISILESSSAFPDNAARREVESLPAVCPS
DG
CTWKGTLKEYEVKMPACGMVTEAPAVGSRPR
SPSSYDLVLHVPLTGAEACLMSVEEETELLLR
S
CHEGRCPLMLTECPACKGLVRLGEKERHLEHECP
ERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCD
GCGKKKIPREKLDQDKIEALSSKVQQLERSIGL
KDLAMADLEQKVLEMEASTYDGVFIWKISDFARK
RQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLN
GDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKV
TLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMN
IASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVD
LTGL*LPPTGVWGLGAARHSRLTEGPPRWARVS
LYKWAGAALGRLGGCRPAAKFTVTGEGATSQSSD
FRDCGGAWQTVLAKGCGGIGRGSSGASQHKLPL
LSCAVKGEALGGGHSEWEHIPAVPM*QEHSGWP
CVPRA*QAETRAAPGEGPPAGQSKEG*AAWFSPL
APGEKGAFLDPWVLVCTELWSVPPWPGWLWERV
WSHAASAQTTVWEVHSTACG*SVRACHPAHEDR
VIKPFKSLWSVASI*FQVACGFQPWQW*PSAGPP
GLSSHRPLPTGCLAPACFSS*PGCVLCSSPDVS
SLFSSLVPSSGMFFPVHLLSWASPGTPVRAE*G
*ASPGPAFLCTGSTEHPGCTRGVLATPRVLAAPP
LWCVFGTVQAGVGTASHSVSGQPCWDLLGICIG
VQDKVLSPLDVFTHCCSLRS*PQKSTENNSGTE
LTDGHCLCSVSVYWA*RASIAEPCCSLSRPHLSS
PGHVAGIEAFRFSLGFRILHLYVSVFTNKNIAL
KMQKPSTGSAAGSWAFDSAWLLLMHLVLGTLHG
GF*LPCVSSLESTFQSLPVSSGFGDWR*PDLLSW
VSCQPGRRVGGF*ASPF*QLPACFAEGLHPVLP
PAPGGGFPSLPSLPSLPVLRVVGIEMLQRAVCE
VKWHRCHVRFSDDSAESGVFTTLTSPLDSLPCEL
PVDVFFSFFFWLLVWVLFCWLVGVPDIPDYGFS
VVSLNCVSVAPDVQKLKPYT*SADLILYEWCYF
RPTQNILTFPEAGNHCHVFV**L*SFVFNILGFY
IWGFVCM*QVLLGHCSLLKQID*GHLFSLKTKW
NCEMRGMDQKKILPQGQRWGRSQHPTKDLNPFW
KVGSGLVGGC*AEEG*GGSWPHREALAWSRV*KW
IN*KTGQSSYLGGPLPEQPDGPPGPHLPAVEME
VWRTWGRAEDRTPHAVRAASGSRLQKGQRSALG
TIP*ALAATPRGSHEADQGP*GTKCSGAAPWAGL
AQPPHGLLGK*QALCKPTEVPSSLPNFGTFHHP
AEKPSLSSHCSLPLSP*QPAINLPSVSIDLPTQ
DIHVNVAFAFTQQDIFMVPVCCSMHWSFIPSNG*
IISCMDAPHLIYPFIRCWTFGWFLLFGYSENAA
VNICEQVFMGT

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