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Sequences

Sequence TRAF2.cAug05

CGGTAGCTGGGCGGGCCCTTAGTTCCGGGCGCGCTGCGACCGTTGGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCC TGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGC AGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGG GCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAG TGATGGATGCACCTGGAAGGGGACCCTGAAAGAATACGAGGTAAAGATGCCTGCGTGTGGCATGGTGACAGAAGCTCCAGCAGTCGGGAGTCGTCCACGC TCCCCAAGCAGTTATGACCTTGTCCTGCACGTTCCTCTCACTGGGGCTGAAGCCTGTCTGATGTCTGTGGAGGAAGAGACGGAGCTGCTCCTTAGAAGCT GCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTGGTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCC GGAGAGAAGCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGAAGGCGCACCACGAGGTCTGCCCCAAGTTCCCCTTAACTTGTGAC GGCTGCGGCAAGAAGAAGATCCCCCGGGAGAAGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCA AGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTCTTGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCAGGAA GCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACACCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAAC GGCGACGGCACCGGGCGAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGA CCTTAATGCTGCTCGACCAGAATAACCGGGAGCACGTGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACATGAA CATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATTCCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGAC CTGACAGGGCTCTAACTGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGCCACCACGCTGGGCCAGGGTCTCAC TGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTGGGAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCTCAGA TTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCATTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTG CTGTCCTGTGCAGTGAAGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGTAGCAGGAGCACAGTGGATGGCCTT GTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCTGCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCT GGCCCCTGGAGAGAAGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGAGCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTC TGGTCCCACGCCGCCTCTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTTGCCATCCAGCTCACGAAGACAGAG TTATTAAACCATTCAAATCTCTGTGGTCAGTGGCATCCATTTGATTCCAGGTTGCCTGCGGCTTCCAGCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCC TGGCCTCTCCTCTCACAGACCCCTCCCCACGGGGTGCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCAGGCTGTGTTCTCTGTTCCTCACCTGATGTAAGC TCCCTCTTCTCGAGCCTTGTTCCCTCTTCTGGAATGTTCTTCCCTGTCCATCTCCTCTCCTGGGCCTCCCCAGGGACTCCTGTGAGAGCAGAATGAGGAT GAGCCAGCCCTGGTCCTGCCTTTCTGTGCACAGGCTCCACCGAGCATCCTGGCTGCACCCGGGGGGTCCTGGCCACACCGAGGGTCCTGGCTGCACCGCC CCTGTGGTGTGTTTTTGGGACAGTGCAGGCAGGCGTAGGCACGGCTTCACACTCGGTGTCAGGGCAGCCGTGCTGGGACCTACTGGGCATCTGTATCGGG GTCCAGGATAAAGTGCTGTCCCCTCTCGACGTCTTCACGCACTGCTGTAGTTTGAGAAGTTGACCACAGAAAAGTACAGAAAATAACTCAGGCACGGAGC TGACCGACGGGCATTGTCTGTGTTCCGTGAGTGTGTACTGGGCCTAAAGAGCAAGCATTGCAGAGCCCTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTC CCCAGGCCACGTGGCGGGAATTGAGGCGTTCCGGTTCTCTTTGGGCTTTAGAATTTTGCATCTGTACGTTTCTGTATTCACAAATAAGAATATTGCATTA AAAATGCAGAAACCATCCACTGGGTCAGCTGCAGGTAGCTGGGCTTTTGACTCAGCCTGGCTACTTTTAATGCATCTTGTGCTGGGCACTCTACATGGTG GATTCTGACTGCCGTGTGTGAGTTCCCTAGAGAGCACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTTTGGTGACTGGAGATAGCCAGACCTGCTCTCGTG GGTCTCCTGTCAACCTGGAAGGAGGGTTGGTGGTTTCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCTTGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCA CCAGCACCAGGGGGAGGGTTCCCATCACTCCCATCCCTCCCATCCCTGCCCGTGCTTCGTGTTGTTGGAATTGAGATGCTGCAGCGTGCTGTGTGTGAAG TGAAGTGGCATCGCTGTCACGTGCGTTTCTCTGATGACTCGGCGGAGTCAGGCGTCTTTACCACGCTTACCTCTCCCTTGGATTCTCTCCCGTGCGAATT GCCTGTTGATGTCTTTTTCTCATTTTTCTTCTGGTTGTTGGTCTGGGTCTTATTCTGCTGGTTGGTAGGAGTTCCTGATATTCCTGATTACGGATTCTCG GTTGTGTCTTTGAACTGCGTTTCTGTTGCTCCTGATGTGCAGAAGCTTAAGCCTTACACATGATCAGCTGATCTTATCCTTTATGAATGGTGCTATTTCC GACCGACTCAGAACATCCTGACCTTCCCTGAGGCTGGAAACCACTGTCATGTTTTCGTCTAATGATTGTGAAGTTTTGTCTTTAACATACTGGGCTTCTA TATTTGGGGATTTGTTTGTATGTGACAGGTTTTGCTGGGTCACTGCAGTTTGTTAAAACAGATTGATTGAGGTCATCTCTTCTCACTTAAAACGAAATGG AACTGTGAAATGAGAGGAATGGACCAAAAAAAAATCCTCCCCCAGGGACAAAGATGGGGAAGAAGCCAACATCCCACAAAGGATTTGAACCCATTTTGGA AGGTGGGAAGTGGGTTGGTGGGTGGCTGCTGAGCAGAAGAGGGCTGAGGGGGAAGCTGGCCACACCGGGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTG GATCAACTGAAAGACGGGGCAGAGCAGCTACCTTGGAGGTCCTTTGCCAGAGCAGCCGGATGGTCCTCCTGGCCCTCACCTCCCTGCAGTGGAGATGGAG GTCTGGAGAACCTGGGGAAGAGCTGAGGACCGCACCCCCCACGCAGTGAGAGCAGCATCGGGCTCCAGGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAA CAATTCCCTAGGCACTGGCTGCCACACCAAGAGGCTCCCACGAAGCAGACCAAGGCCCTTGAGGGACAAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCT GGCCCAGCCGCCTCATGGCCTTCTGGGGAAGTGACAGGCTCTGTGCAAACCCACAGAGGTACCATCGTCACTACCTAATTTTGGGACATTTCATCACCCT GCAGAGAAACCTTCCCTTAGCAGTCACTGTTCCCTCCCGCTCAGCCCCTGACAGCCAGCCATAAACCTACCTTCTGTCTCTATAGATTTGCCTACTCAGG ACATTCATGTGAATGTGGCCTTTGCTTTTACTCAGCAAGATATTTTCATGGTTCCTGTGTGTTGCAGCATGCATTGGAGTTTCATTCCTTCTAATGGCTG AATAATCTCTTGTATGGATGCACCACACCTTATTTATCCATTCATCCGTTGCTGGACATTTGGGTGGTTTCTACTTTTTGGCTACTCTGAAAATGCTGCT GTGAACATTTGTGAACAAGTTTTTATGGGGACGTG

TRAF2-3 (forward primer)
TRAF2-4 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

R*LGGPLVPGALRPLGLCSRGSQLSWLQLA*PP LAPWSCYSPASPRPSWGPSWKPSTCAPPAETSSA GPSRRSVATGTAPSAWPASSALGLRTVLPVFTR AYMKKAFLF*KAVRPSQIMLPAGRWRACRPSVP VMDAPGRGP*KNTR*RCLRVAW*QKLQQSGVVHA PQAVMTLSCTFLSLGLKPV*CLWRKRRSCSLEA ATKAAARSC*PNVPRAKAWSALVKRSATWSTSA RREA*AAGIAGHPAAEQT*RRTTRSAPSSP*LVT AAARRRSPGRSWTKTRLKP*VARCSSWRGALAS RTWRWLTWSRRSWRWRHPPTMGSSSGRSQTSPG SARKLWLAAYPPSSPQPSTPAGTATRCVCVST*T ATAPGEEHTCPSSLW**RARMTPCCGGPSTRR* P*CCSTRITGST*LTPSGPT*LHPLFRGQSTT* TSQAAAPSSAPSPRWRQRIPTCGTMPSSSRPLWT *QGSNCPLLVSGGWGQPGTAGSRRGHHAGPGSH CTSGQGPRLGAWEGVGLQPSSLSRGKEPPASPQ ISETAEGLGRRS*PRAVVALAEGLRVLPSTSCPC CPVQ*RERPWVGDTQSGSTSQQCPCSRSTVDGL VSLGHDRQKRGLLQEKGLLLARARKAEQLGSPL WPLERREHS*TPGCLSAQSSGLCHLGQAGCGRGS GPTPPLLRPLCGRCTAQPAGKV*ELAIQLTKTE LLNHSNLCGQWHPFDSRLPAASSLGSGDPLRVL LASPLTDPSPRGA*PLPASLASQAVFSVPHLM*A PSSRALFPLLECSSLSISSPGPPQGLL*EQNED EPALVLPFCAQAPPSILAAPGGSWPHRGSWLHR PCGVFLGQCRQA*ARLHTRCQGSRAGTYWASVSG SRIKCCPLSTSSRTAVV*EVDHRKVQKITQARS *PTGIVCVP*VCTGPKEQALQSPAVPFPGLTSA PQATWRELRRSGSLWALEFCICTFLYSQIRILH* KCRNHPLGQLQVAGLLTQPGYF*CILCWALYMV DSDCRV*VP*RAPSSLFPCLQGLVTGDSQTCSR GSPVNLEGGLVVSRPPLFSSCQLALQRGCIRSFH QHQGEGSHHSHPSHPCPCFVLLELRCCSVLCVK *SGIAVTCVSLMTRRSQASLPRLPLPWILSRAN CLLMSFSHFSSGCWSGSYSAGW*EFLIFLITDSR LCL*TAFLLLLMCRSLSLTHDQLILSFMNGAIS DRLRTS*PSLRLETTVMFSSNDCEVLSLTYWAS IFGDLFVCDRFCWVTAVC*NRLIEVISSHLKRNG TVK*EEWTKKKSSPRDKDGEEANIPQRI*THFG RWEVGWWVAAEQKRAEGEAGHTGRPWHGAESKS GSTERRGRAATLEVLCQSSRMVLLALTSLQWRWR SGEPGEELRTAPPTQ*EQHRAPGCRRGRGLHSE QFPRHWLPHQEAPTKQTKALEGQNALEQPRGQA WPSRLMAFWGSDRLCANPQRYHRHYLILGHFITL QRNLPLAVTVPSRSAPDSQP*TYLLSL*ICLLR TFM*MWPLLLLSKIFSWFLCVAACIGVSFLLMA E*SLVWMHHTLFIHSSVAGHLGGFYFLATLKMLL *TFVNKFLWGR

Frame 1

GSWAGP*FRARCDRWGFVRGGHSSHGCS*RDPP WLPGVATARLLQDPPGDQAGSQVPVLRLQKRPP QALPGAVWPPVLLLLPGQHPQLWASELCCLCSRG HI*RRHFYFRKQFGLPR*CCPQGGGEPAGRLSQ *WMHLEGDPERIRGKDACVWHGDRSSSSRESST LPKQL*PCPARSSHWG*SLSDVCGGRDGAAP*KL PRRPLPAHADRMSRVQRPGPPW*KGAPPGARVP GEKPELPALPGTLLRSRREGAPRGLPQVPLNL* RLRQEEDPPGEAGPRQD*SPE*QGAAAGEEHWPQ GPGDG*LGAEGLGDGGIHLRWGLHLEDLRLRQE APGSCGWPHTRHLLPSLLHQQVRLQDVSAYLPE RRRHRARNTPVPLLCGDEGPE*RPAAVALQPEGD LNAARPE*PGARD*RLQARRDFILFSEASQRHE HRKRLPPLLPRLQDGGKEFLRAGRCHLHQGHCG PDRALTAPYWCLGVGGSQAQPAHGGATTLGQGLT VQVGRGRAWALGRVSACSQVHCHGGRSHQPVLR FQRLRRGLADGLSQGLWWHWPRVFGCFPAQAAL AVLCSEGRGPGWGTLRVGAHPSSAHVAGAQWMAL CPSGMTGRNEGCSRRRASCWPEQGRLSSLVLPS GPWREGSIPRPLGACLHRALVCATLARLAVGEG LVPRRLCSDHCVGGAQHSLRVKCESLPSSSRRQS Y*TIQISVVSGIHLIPGCLRLPALAVVTLCGSS WPLLSQTPPHGVPSPCLLL*LARLCSLFLT*CK LPLLEPCSLFWNVLPCPSPLLGLPRDSCESRMRM SQPWSCLSVHRLHRASWLHPGGPGHTEGPGCTA PVVCFWDSAGRRRHGFTLGVRAAVLGPTGHLYR GPG*SAVPSRRLHALL*FEKLTTEKYRK*LRHGA DRRALSVFRECVLGLKSKHCRALLFPFPASPQL PRPRGGN*GVPVLFGL*NFASVRFCIHK*EYCI KNAETIHWVSCR*LGF*LSLATFNASCAGHSTWW ILTAVCEFPREHLPVSSRVFRVW*LEIARPALV GLLSTWKEGWWFLGLPFLAVASLLCRGAASGPS TSTRGRVPITPIPPIPARASCCWN*DAAACCV*S EVASLSRAFL**LGGVRRLYHAYLSLGFSPVRI AC*CLFLIFLLVVGLGLILLVGRSS*YS*LRIL GCVFELRFCCS*CAEA*ALHMIS*SYPL*MVLFP TDSEHPDLP*GWKPLSCFRLMIVKFCL*HTGLL YLGICLYVTGFAGSLQFVKTD*LRSSLLT*NEM EL*NERNGPKKNPPPGTKMGKKPTSHKGFEPILE GGKWVGGWLLSRRGLRGKLATPGGPGMEQSLKV DQLKDGAEQLPWRSFARAAGWSSWPSPPCSGDG GLENLGKS*GPHPPRSESSIGLQAAEGAEVCTRN NSLGTGCHTKRLPRSRPRPLRDKMLWSSPVGRP GPAASWPSGEVTGSVQTHRGTIVTT*FWDISSP CRETFP*QSLFPPAQPLTASHKPTFCLYRFAYSG HSCECGLCFYSARYFHGSCVLQHALEFHSF*WL NNLLYGCTTPYLSIHPLLDIWVVSTFWLL*KCC CEHL*TSFYGDV

Frame 2

VAGRALSSGRAATVGALFAGVTALMAAASVTPP GSLELLQPGFSKTLLGTKLEAKYLCSACRNVLR RPFQAQCGHRYCSFCLASILSSGPQNCAACVHE GIYEEGISILESSSAFPDNAARREVESLPAVCPS DGCTWKGTLKEYEVKMPACGMVTEAPAVGSRPR SPSSYDLVLHVPLTGAEACLMSVEEETELLLRS CHEGRCPLMLTECPACKGLVRLGEKERHLEHECP ERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCD GCGKKKIPREKLDQDKIEALSSKVQQLERSIGL KDLAMADLEQKVLEMEASTYDGVFIWKISDFARK RQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLN GDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKV TLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMN IASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVD LTGL*LPPTGVWGLGAARHSRLTEGPPRWARVS LYKWAGAALGRLGGCRPAAKFTVTGEGATSQSSD FRDCGGAWQTVLAKGCGGIGRGSSGASQHKLPL LSCAVKGEALGGGHSEWEHIPAVPM*QEHSGWP CVPRA*QAETRAAPGEGPPAGQSKEG*AAWFSPL APGEKGAFLDPWVLVCTELWSVPPWPGWLWERV WSHAASAQTTVWEVHSTACG*SVRACHPAHEDR VIKPFKSLWSVASI*FQVACGFQPWQW*PSAGPP GLSSHRPLPTGCLAPACFSS*PGCVLCSSPDVS SLFSSLVPSSGMFFPVHLLSWASPGTPVRAE*G *ASPGPAFLCTGSTEHPGCTRGVLATPRVLAAPP LWCVFGTVQAGVGTASHSVSGQPCWDLLGICIG VQDKVLSPLDVFTHCCSLRS*PQKSTENNSGTE LTDGHCLCSVSVYWA*RASIAEPCCSLSRPHLSS PGHVAGIEAFRFSLGFRILHLYVSVFTNKNIAL KMQKPSTGSAAGSWAFDSAWLLLMHLVLGTLHG GF*LPCVSSLESTFQSLPVSSGFGDWR*PDLLSW VSCQPGRRVGGF*ASPF*QLPACFAEGLHPVLP PAPGGGFPSLPSLPSLPVLRVVGIEMLQRAVCE VKWHRCHVRFSDDSAESGVFTTLTSPLDSLPCEL PVDVFFSFFFWLLVWVLFCWLVGVPDIPDYGFS VVSLNCVSVAPDVQKLKPYT*SADLILYEWCYF RPTQNILTFPEAGNHCHVFV**L*SFVFNILGFY IWGFVCM*QVLLGHCSLLKQID*GHLFSLKTKW NCEMRGMDQKKILPQGQRWGRSQHPTKDLNPFW KVGSGLVGGC*AEEG*GGSWPHREALAWSRV*KW IN*KTGQSSYLGGPLPEQPDGPPGPHLPAVEME VWRTWGRAEDRTPHAVRAASGSRLQKGQRSALG TIP*ALAATPRGSHEADQGP*GTKCSGAAPWAGL AQPPHGLLGK*QALCKPTEVPSSLPNFGTFHHP AEKPSLSSHCSLPLSP*QPAINLPSVSIDLPTQ DIHVNVAFAFTQQDIFMVPVCCSMHWSFIPSNG* IISCMDAPHLIYPFIRCWTFGWFLLFGYSENAA VNICEQVFMGT

Sequence TRAF2.bAug05

CGGTAGCTGGGCGGGCCCTTAGTTCCGGGCGCGCTGCGACCGTTGGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCC TGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGC AGGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGG GCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCCTGCCGGCCGTCTGTCCCAG TGATGGATGCACCTGGAAGGGGACCCTGAAAGAATACGAGAGCTGCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTG GTCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAAGCCTGAGCTGCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGA AGTTTCAGGACCACGTCAAGACTTGTGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCTGCCTCGAGACGGTAGAGGGTGAGAAACAGCAGGA GCACGAGGTGCAGTGGCTGCGGGAGCACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAGACCAGAGCCACGCGGGGTCAGAG CTCCTGCAGAGGTGCGAGAGCCTGGAGAAGAAGACGGCCACTTTTGAGAACATTGTCTGCGTCCTGAACCGGGAGGTGGAGAGGGTGGCCATGACTGCCG AGGCCTGCAGCCGGCAGCACCGGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGCAGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGC GATGGCTGACTTGGAGCAGAAGGTCTTGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCAGGAAGCGCCAGGAA GCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACACCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCA CCGGGCGAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGCGGTGGCCCTTCAACCAGAAGGTGACCTTAATGCT GCTCGACCAGAATAACCGGGAGCACGTGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACATGAACATCGCAAGC GGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATTCCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGC TCTAACTGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGCCACCACGCTGGGCCAGGGTCTCACTGTACAAGTG GGCAGGGGCCGCGCTTGGGCGCTTGGGAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCTCAGATTTCAGAGAC TGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCATTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTG CAGTGAAGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGTAGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCG GGCATGACAGGCAGAAACGAGGGCTGCTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCCCTCTGGCCCCTGGA GAGAAGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGAGCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGAGGGTCTGGTCCCACG CCGCCTCTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTTGCCATCCAGCTCACGAAGACAGAGTTATTAAACC ATTCAAATCTCTGTGGTCAGTGGCATCCATTTGATTCCAGGTTGCCTGCGGCTTCCAGCCTTGGCAGTGGTGACCCTCTGCGGGTCCTCCTGGCCTCTCC TCTCACAGACCCCTCCCCACGGGGTGCCTAGCCCCTGCCTGCTTCTCTAGCTAGCCAGGCTGTGTTCTCTGTTCCTCACCTGATGTAAGCTCCCTCTTCT CGAGCCTTGTTCCCTCTTCTGGAATGTTCTTCCCTGTCCATCTCCTCTCCTGGGCCTCCCCAGGGACTCCTGTGAGAGCAGAATGAGGATGAGCCAGCCC TGGTCCTGCCTTTCTGTGCACAGGCTCCACCGAGCATCCTGGCTGCACCCGGGGGGTCCTGGCCACACCGAGGGTCCTGGCTGCACCGCCCCTGTGGTGT GTTTTTGGGACAGTGCAGGCAGGCGTAGGCACGGCTTCACACTCGGTGTCAGGGCAGCCGTGCTGGGACCTACTGGGCATCTGTATCGGGGTCCAGGATA AAGTGCTGTCCCCTCTCGACGTCTTCACGCACTGCTGTAGTTTGAGAAGTTGACCACAGAAAAGTACAGAAAATAACTCAGGCACGGAGCTGACCGACGG GCATTGTCTGTGTTCCGTGAGTGTGTACTGGGCCTAAAGAGCAAGCATTGCAGAGCCCTGCTGTTCCCTTTCCCGGCCTCACCTCAGCTCCCCAGGCCAC GTGGCGGGAATTGAGGCGTTCCGGTTCTCTTTGGGCTTTAGAATTTTGCATCTGTACGTTTCTGTATTCACAAATAAGAATATTGCATTAAAAATGCAGA AACCATCCACTGGGTCAGCTGCAGGTAGCTGGGCTTTTGACTCAGCCTGGCTACTTTTAATGCATCTTGTGCTGGGCACTCTACATGGTGGATTCTGACT GCCGTGTGTGAGTTCCCTAGAGAGCACCTTCCAGTCTCTTCCCGTGTCTTCAGGGTTTGGTGACTGGAGATAGCCAGACCTGCTCTCGTGGGTCTCCTGT CAACCTGGAAGGAGGGTTGGTGGTTTCTAGGCCTCCCCTTTTTAGCAGTTGCCAGCTTGCTTTGCAGAGGGGCTGCATCCGGTCCTTCCACCAGCACCAG GGGGAGGGTTCCCATCACTCCCATCCCTCCCATCCCTGCCCGTGCTTCGTGTTGTTGGAATTGAGATGCTGCAGCGTGCTGTGTGTGAAGTGAAGTGGCA TCGCTGTCACGTGCGTTTCTCTGATGACTCGGCGGAGTCAGGCGTCTTTACCACGCTTACCTCTCCCTTGGATTCTCTCCCGTGCGAATTGCCTGTTGAT GTCTTTTTCTCATTTTTCTTCTGGTTGTTGGTCTGGGTCTTATTCTGCTGGTTGGTAGGAGTTCCTGATATTCCTGATTACGGATTCTCGGTTGTGTCTT TGAACTGCGTTTCTGTTGCTCCTGATGTGCAGAAGCTTAAGCCTTACACATGATCAGCTGATCTTATCCTTTATGAATGGTGCTATTTCCGACCGACTCA GAACATCCTGACCTTCCCTGAGGCTGGAAACCACTGTCATGTTTTCGTCTAATGATTGTGAAGTTTTGTCTTTAACATACTGGGCTTCTATATTTGGGGA TTTGTTTGTATGTGACAGGTTTTGCTGGGTCACTGCAGTTTGTTAAAACAGATTGATTGAGGTCATCTCTTCTCACTTAAAACGAAATGGAACTGTGAAA TGAGAGGAATGGACCAAAAAAAAATCCTCCCCCAGGGACAAAGATGGGGAAGAAGCCAACATCCCACAAAGGATTTGAACCCATTTTGGAAGGTGGGAAG TGGGTTGGTGGGTGGCTGCTGAGCAGAAGAGGGCTGAGGGGGAAGCTGGCCACACCGGGAGGCCCTGGCATGGAGCAGAGTCTAAAAGTGGATCAACTGA AAGACGGGGCAGAGCAGCTACCTTGGAGGTCCTTTGCCAGAGCAGCCGGATGGTCCTCCTGGCCCTCACCTCCCTGCAGTGGAGATGGAGGTCTGGAGAA CCTGGGGAAGAGCTGAGGACCGCACCCCCCACGCAGTGAGAGCAGCATCGGGCTCCAGGCTGCAGAAGGGGCAGAGGTCTGCACTCGGAACAATTCCCTA GGCACTGGCTGCCACACCAAGAGGCTCCCACGAAGCAGACCAAGGCCCTTGAGGGACAAAATGCTCTGGAGCAGCCCCGTGGGCAGGCCTGGCCCAGCCG CCTCATGGCCTTCTGGGGAAGTGACAGGCTCTGTGCAAACCCACAGAGGTACCATCGTCACTACCTAATTTTGGGACATTTCATCACCCTGCAGAGAAAC CTTCCCTTAGCAGTCACTGTTCCCTCCCGCTCAGCCCCTGACAGCCAGCCATAAACCTACCTTCTGTCTCTATAGATTTGCCTACTCAGGACATTCATGT GAATGTGGCCTTTGCTTTTACTCAGCAAGATATTTTCATGGTTCCTGTGTGTTGCAGCATGCATTGGAGTTTCATTCCTTCTAATGGCTGAATAATCTCT TGTATGGATGCACCACACCTTATTTATCCATTCATCCGTTGCTGGACATTTGGGTGGTTTCTACTTTTTGGCTACTCTGAAAATGCTGCTGTGAACATTT GTGAACAAGTTTTTATGGGGACGTG

TRAF2-3 (forward primer)
TRAF2-4 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

R*LGGPLVPGALRPLGLCSRGSQLSWLQLA*PP LAPWSCYSPASPRPSWGPSWKPSTCAPPAETSSA GPSRRSVATGTAPSAWPASSALGLRTVLPVFTR AYMKKAFLF*KAVRPSQIMLPAGRWRACRPSVP VMDAPGRGP*KNTRAATKAAARSC*PNVPRAKAW SALVKRSATWSTSARREA*AAGIAGHPAAEQT* SFRTTSRLVASVESLADSTPSAASRR*RVRNSR STRCSGCGSTWPCY*ARCWRQSPSWETRATRGQS SCRGARAWRRRRPLLRTLSAS*TGRWRGWP*LP RPAAGSTGWTKTRLKP*VARCSSWRGALASRTW RWLTWSRRSWRWRHPPTMGSSSGRSQTSPGSARK LWLAAYPPSSPQPSTPAGTATRCVCVST*TATA PGEEHTCPSSLW**RARMTPCCGGPSTRR*P*C CSTRITGST*LTPSGPT*LHPLFRGQSTT*TSQA AAPSSAPSPRWRQRIPTCGTMPSSSRPLWT*QG SNCPLLVSGGWGQPGTAGSRRGHHAGPGSHCTS GQGPRLGAWEGVGLQPSSLSRGKEPPASPQISET AEGLGRRS*PRAVVALAEGLRVLPSTSCPCCPV Q*RERPWVGDTQSGSTSQQCPCSRSTVDGLVSL GHDRQKRGLLQEKGLLLARARKAEQLGSPLWPLE RREHS*TPGCLSAQSSGLCHLGQAGCGRGSGPT PPLLRPLCGRCTAQPAGKV*ELAIQLTKTELLN HSNLCGQWHPFDSRLPAASSLGSGDPLRVLLASP LTDPSPRGA*PLPASLASQAVFSVPHLM*APSS RALFPLLECSSLSISSPGPPQGLL*EQNEDEPA LVLPFCAQAPPSILAAPGGSWPHRGSWLHRPCGV FLGQCRQA*ARLHTRCQGSRAGTYWASVSGSRI KCCPLSTSSRTAVV*EVDHRKVQKITQARS*PT GIVCVP*VCTGPKEQALQSPAVPFPGLTSAPQAT WRELRRSGSLWALEFCICTFLYSQIRILH*KCR NHPLGQLQVAGLLTQPGYF*CILCWALYMVDSD CRV*VP*RAPSSLFPCLQGLVTGDSQTCSRGSPV NLEGGLVVSRPPLFSSCQLALQRGCIRSFHQHQ GEGSHHSHPSHPCPCFVLLELRCCSVLCVK*SG IAVTCVSLMTRRSQASLPRLPLPWILSRANCLLM SFSHFSSGCWSGSYSAGW*EFLIFLITDSRLCL *TAFLLLLMCRSLSLTHDQLILSFMNGAISDRL RTS*PSLRLETTVMFSSNDCEVLSLTYWASIFGD LFVCDRFCWVTAVC*NRLIEVISSHLKRNGTVK *EEWTKKKSSPRDKDGEEANIPQRI*THFGRWE VGWWVAAEQKRAEGEAGHTGRPWHGAESKSGSTE RRGRAATLEVLCQSSRMVLLALTSLQWRWRSGE PGEELRTAPPTQ*EQHRAPGCRRGRGLHSEQFP RHWLPHQEAPTKQTKALEGQNALEQPRGQAWPSR LMAFWGSDRLCANPQRYHRHYLILGHFITLQRN LPLAVTVPSRSAPDSQP*TYLLSL*ICLLRTFM *MWPLLLLSKIFSWFLCVAACIGVSFLLMAE*SL VWMHHTLFIHSSVAGHLGGFYFLATLKMLL*TF VNKFLWGR

Frame 1

GSWAGP*FRARCDRWGFVRGGHSSHGCS*RDPP WLPGVATARLLQDPPGDQAGSQVPVLRLQKRPP QALPGAVWPPVLLLLPGQHPQLWASELCCLCSRG HI*RRHFYFRKQFGLPR*CCPQGGGEPAGRLSQ *WMHLEGDPERIRELPRRPLPAHADRMSRVQRP GPPW*KGAPPGARVPGEKPELPALPGTLLRSRRE VSGPRQDLWQVSSPLQIPRHRLPRDGRG*ETAG ARGAVAAGAPGHATELGAGGKAPLGRPEPRGVR APAEVREPGEEDGHF*EHCLRPEPGGGEGGHDCR GLQPAAPAGPRQD*SPE*QGAAAGEEHWPQGPG DG*LGAEGLGDGGIHLRWGLHLEDLRLRQEAPG SCGWPHTRHLLPSLLHQQVRLQDVSAYLPERRRH RARNTPVPLLCGDEGPE*RPAAVALQPEGDLNA ARPE*PGARD*RLQARRDFILFSEASQRHEHRK RLPPLLPRLQDGGKEFLRAGRCHLHQGHCGPDRA LTAPYWCLGVGGSQAQPAHGGATTLGQGLTVQV GRGRAWALGRVSACSQVHCHGGRSHQPVLRFQR LRRGLADGLSQGLWWHWPRVFGCFPAQAALAVLC SEGRGPGWGTLRVGAHPSSAHVAGAQWMALCPS GMTGRNEGCSRRRASCWPEQGRLSSLVLPSGPW REGSIPRPLGACLHRALVCATLARLAVGEGLVPR RLCSDHCVGGAQHSLRVKCESLPSSSRRQSY*T IQISVVSGIHLIPGCLRLPALAVVTLCGSSWPL LSQTPPHGVPSPCLLL*LARLCSLFLT*CKLPLL EPCSLFWNVLPCPSPLLGLPRDSCESRMRMSQP WSCLSVHRLHRASWLHPGGPGHTEGPGCTAPVV CFWDSAGRRRHGFTLGVRAAVLGPTGHLYRGPG* SAVPSRRLHALL*FEKLTTEKYRK*LRHGADRR ALSVFRECVLGLKSKHCRALLFPFPASPQLPRP RGGN*GVPVLFGL*NFASVRFCIHK*EYCIKNAE TIHWVSCR*LGF*LSLATFNASCAGHSTWWILT AVCEFPREHLPVSSRVFRVW*LEIARPALVGLL STWKEGWWFLGLPFLAVASLLCRGAASGPSTSTR GRVPITPIPPIPARASCCWN*DAAACCV*SEVA SLSRAFL**LGGVRRLYHAYLSLGFSPVRIAC* CLFLIFLLVVGLGLILLVGRSS*YS*LRILGCVF ELRFCCS*CAEA*ALHMIS*SYPL*MVLFPTDS EHPDLP*GWKPLSCFRLMIVKFCL*HTGLLYLG ICLYVTGFAGSLQFVKTD*LRSSLLT*NEMEL*N ERNGPKKNPPPGTKMGKKPTSHKGFEPILEGGK WVGGWLLSRRGLRGKLATPGGPGMEQSLKVDQL KDGAEQLPWRSFARAAGWSSWPSPPCSGDGGLEN LGKS*GPHPPRSESSIGLQAAEGAEVCTRNNSL GTGCHTKRLPRSRPRPLRDKMLWSSPVGRPGPA ASWPSGEVTGSVQTHRGTIVTT*FWDISSPCRET FP*QSLFPPAQPLTASHKPTFCLYRFAYSGHSC ECGLCFYSARYFHGSCVLQHALEFHSF*WLNNL LYGCTTPYLSIHPLLDIWVVSTFWLL*KCCCEHL *TSFYGDV

Frame 2

VAGRALSSGRAATVGALFAGVTALMAAASVTPP GSLELLQPGFSKTLLGTKLEAKYLCSACRNVLR RPFQAQCGHRYCSFCLASILSSGPQNCAACVHE GIYEEGISILESSSAFPDNAARREVESLPAVCPS DGCTWKGTLKEYESCHEGRCPLMLTECPACKGL VRLGEKERHLEHECPERSLSCRHCRAPCCGADV KFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQE HEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSE LLQRCESLEKKTATFENIVCVLNREVERVAMTA EACSRQHRLDQDKIEALSSKVQQLERSIGLKDLA MADLEQKVLEMEASTYDGVFIWKISDFARKRQE AVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDG TGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLML LDQNNREHVIDAFRPDVTSSSFQRPVNDMNIAS GCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTG L*LPPTGVWGLGAARHSRLTEGPPRWARVSLYKW AGAALGRLGGCRPAAKFTVTGEGATSQSSDFRD CGGAWQTVLAKGCGGIGRGSSGASQHKLPLLSC AVKGEALGGGHSEWEHIPAVPM*QEHSGWPCVPR A*QAETRAAPGEGPPAGQSKEG*AAWFSPLAPG EKGAFLDPWVLVCTELWSVPPWPGWLWERVWSH AASAQTTVWEVHSTACG*SVRACHPAHEDRVIKP FKSLWSVASI*FQVACGFQPWQW*PSAGPPGLS SHRPLPTGCLAPACFSS*PGCVLCSSPDVSSLF SSLVPSSGMFFPVHLLSWASPGTPVRAE*G*ASP GPAFLCTGSTEHPGCTRGVLATPRVLAAPPLWC VFGTVQAGVGTASHSVSGQPCWDLLGICIGVQD KVLSPLDVFTHCCSLRS*PQKSTENNSGTELTDG HCLCSVSVYWA*RASIAEPCCSLSRPHLSSPGH VAGIEAFRFSLGFRILHLYVSVFTNKNIALKMQ KPSTGSAAGSWAFDSAWLLLMHLVLGTLHGGF*L PCVSSLESTFQSLPVSSGFGDWR*PDLLSWVSC QPGRRVGGF*ASPF*QLPACFAEGLHPVLPPAP GGGFPSLPSLPSLPVLRVVGIEMLQRAVCEVKWH RCHVRFSDDSAESGVFTTLTSPLDSLPCELPVD VFFSFFFWLLVWVLFCWLVGVPDIPDYGFSVVS LNCVSVAPDVQKLKPYT*SADLILYEWCYFRPTQ NILTFPEAGNHCHVFV**L*SFVFNILGFYIWG FVCM*QVLLGHCSLLKQID*GHLFSLKTKWNCE MRGMDQKKILPQGQRWGRSQHPTKDLNPFWKVGS GLVGGC*AEEG*GGSWPHREALAWSRV*KWIN* KTGQSSYLGGPLPEQPDGPPGPHLPAVEMEVWR TWGRAEDRTPHAVRAASGSRLQKGQRSALGTIP* ALAATPRGSHEADQGP*GTKCSGAAPWAGLAQP PHGLLGK*QALCKPTEVPSSLPNFGTFHHPAEK PSLSSHCSLPLSP*QPAINLPSVSIDLPTQDIHV NVAFAFTQQDIFMVPVCCSMHWSFIPSNG*IIS CMDAPHLIYPFIRCWTFGWFLLFGYSENAAVNI CEQVFMGT