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Sequences

Sequence FASTK.pAug05

AGTGCCTGCCGGGAGCCACGTCTCCGAAGACCGATAGCTGCTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG GCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGG CGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGTGTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCC CGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTCGGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACT TAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGTGAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCC TCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTCGGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTA ATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGCCTGCGTTTCTCCACCGGTGAAATGAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCT GCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCT GTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACC CCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACT GCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGGTACAGAA GTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCC CTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCA GTGGTACGCAGCCAGGATGGCTGGCTGGGCCCCTGAGGGCTGGAGAGGCAGGGGAGCAGGGTGGCCTGCAGCCCAGAGCCCCAGTCCCCGCCTCCCCACA GGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAA GGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCAAGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCT GGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAGCTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTG CCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTCCTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTG AGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGCCTCCAGGCTACTGCACAGGTGAGCAAGGGGCAGGCGGCAGGCCCGG GGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTCCCCTCCAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGA CCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGG GGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCACGTCCCTCCCCCTGCAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGC CGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGGTGAGTGGCCCTGGCCTGGCCCGTGGCGCAT CCTCACTCCCAGCCTGCGGGTAACCTGCCGCCCTCTCCTCTCCCCACAGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGC TACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGG ATGATTTGCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCC

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESCRGG ACGEGCGRAAGAGVSGSGHGLLASLGRGGCWGP RLRRLRAIAKRCTRAPRAPCEVGAAFSFHG*GD LERRSHLSKVTPLVSGSAQHGTQHRPAAEPAFHP LCRRRLLWEREVVGRVHAGAAWAPDWARPLTRL MTSGLT*PLRCSGPAFLHR*NEGLHHPTPCFES CSLLRPPLLGCLACC*SLQYSPAVWGPANGGTGL LEEAPVQVLCKDCSGFWNRRRALGSCCAGWART PARCAPTTTRWRFVVWASSWGLGHGPLLWSRSH CRT*VSSSSETAPPLTFTPSTCVCTLQSYLVVQK LVLPFGRLNYLPLEQQFMPCLERILAREAGVAP LATVNILMSLCQLRCLPFRALHFVFSPGFINYI SGTQPGWLAGPLRAGEAGEQGGLQPRAPVPASPQ APLML*LCVATSPCWTRPWSWSSQDTGVPAFPE GSKCPSFPSLSSPTVPAASTGGWGSLGQGEGPA WDQQSGKARGTMKRQS*GALGHHKTGP*SSRGLC PGWWH*TGTD*DEVPGPSCYWCPFSSVTRT**L RGCASCWGRRNTART*LCLQATAQVSKGQAAGP GRRSPG*GRRPCSPPDFLLCASSSGAVLPVRTQD PFLPYPPRSCPQGQAASSATTRDPAQR*RRQGG GALATLPAMP*ATSLPLQGGAGVAGTLAFLPGR PGAAGLEGPEGAAPRPDGLPAPAGEWPWPGPWRI LTPSLRVTCRPLLSPQLPFEELESQRGLPQLKS YLRQKLQALGLRWGPEGG*GVDVGFRMAPPWGV DDLHFGSLCFDFSLKFLSF

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHVGV APAGRAAGGQRELVLAAAATVSWRPWAGVGVGAP DFADSAPSQSGALGLHARPARWALRFHFTGEET *RDEATCPRSRRW*VGAPSMEPSTVQPRSPRST LCAAAASCGRGRWSGECTPVPPGLQTGRDH*PG* *PRA*LNPFAALGLRFSTGEMRVSITQLHASSP ALCSDLPCSAVWPAADPSSTALLFGAQQMGGPA CWRRPQCRSCARTAAASGTGEEPWGAAALAGPEP QQGARPPLLGGASSSGPALGVSATAPSCGAGHT AGLESAHHPKLPLL*HSHHPRVSAPCSLTWWYR SWSCPLGD*TTCPWNSSLCPALRGSWLGKQGWHP WLQSTS*CHCANCGACPSEPCTLFFPLASSTTS VVRSQDGWLGP*GLERQGSRVACSPEPQSPPPH RHPSCSDCASLPLPAGHGRGAGAPRIPGSPPSPK AASAHLSPASHHRPCPLQVQVDGAAWGKGRGQP GTSRVEKPEGP*SAKAKELSATIKLGPEAAEGF AQGGGTRLGQIRMRCRALPATGVLSPQSQGHSS* GVAPAAGGGEIPPGPDCASRLLHR*ARGRRQAR GDGALAKAAGPAPLQTSCCAPAALVLCFP*GPR TPSCHTHQGPAHRARLPLAPLLETLPRGKGGRVG EPWPPCPPCPEPRPSPCRVVLVLRERWHFCRDG RVLLGSRALRERHLGLMGYQLLPVSGPGLARGA SSLPACG*PAALSSPHSYPSRNWSPREACPSSRA T*GRSSRPWACAGGLKGAEGLMWGSGWPPHGGW MICTLVPCVLISH*SSFPS

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVM*GW RLRGGLREGSGSWC*RQRPRSPGVPGPGWVLGP PTSPTPRHRKAVHSGSTRALRGGRCVFISRVRRL RETKPLVQGHAAGEWERPAWNPAPSSRGARVPP SVPPPPPVGEGGGRESARRCRLGSRLGATTNPV NDLGLNLTPSLLWACVSPPVK*GSPSPNSMLRVL LSAQTSPARLSGLLLIPPVQPCCLGPSKWGDRP VGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQN PSKVRAHHYSVALRRLGQLLGSRPRPPPVEQVTL QDLSQLIIRNCPSFDIHTIHVCLHLAVLLGGTE VGPALWATELPAPGTAVYALP*EDPGSGSRGGT PGYSQHLDVTVPTAVPALQSPALCFFPWLHQLHQ WYAARMAGWAPEGWRGRGAGWPAAQSPSPRLPT GTPHALIVRRYLSLLDTAVELELPGYRGPRLPR RQQVPIFPQPLITDRARCKYRWMGQPGARGGASL GPAEWKSQRDHEAPKLRSSRPP*NWALKQQRAL PRVVALDWDRLG*GAGPFLLLVSFLLSHKDIVA EGLRQLLGEEKYRQDLTVPPGYCTGEQGAGGRPG ETEPWLRPPALLPSRLPAVRQQLWCCASREDPG PLPAIPTKVLPTGPGCL*RHYSRPCPEVKEAGW GSPGHLARHALSHVPPPAGWCWCCGNAGISAGTA GCCWARGP*GSGT*A*WATSSCR*VALAWPVAH PHSQPAGNLPPSPLPTATLRGTGVPERPAPAQE LPEAEAPGPGPALGA*RGLRG*CGVQDGPPMGGG *FALWFPVF*FLIKVPFLP

Sequence FASTK.oAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTAT GCCCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTC AGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGTACGCAGCCAGGATGGCTGGCTGGGCCCCTGAGGGCTGGAGAGGCAGGGG AGCAGGGTGGCCTGCAGCCCAGAGCCCCAGTCCCCGCCTCCCCACAGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAG CTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTC CTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGGTGAGCAAGGGGCAGGCGGCAGGCCCGGGGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTCCCCTCCAGACTTCCTGC TGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAG CGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCACGTCCCTCCCCCTGCAG GGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGC TACCAGCTCCTGCCGGTGAGTGGCCCTGGCCTGGCCCGTGGCGCATCCTCACTCCCAGCCTGCGGGTAACCTGCCGCCCTCTCCTCTCCCCACAGCTACC CTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGC TGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCT TCCC

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPF RALHFVFSPGFINYISGTQPGWLAGPLRAGEAG EQGGLQPRAPVPASPQAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STGGWGSLGQGEGPAWDQQSGKARGTMKRQS*G ALGHHKTGP*SSRGLCPGWWH*TGTD*DEVPGPS CYWCPFSSVTRT**LRGCASCWGRRNTART*LC LQATAQVSKGQAAGPGRRSPG*GRRPCSPPDFL LCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASS ATTRDPAQR*RRQGGGALATLPAMP*ATSLPLQ GGAGVAGTLAFLPGRPGAAGLEGPEGAAPRPDG LPAPAGEWPWPGPWRILTPSLRVTCRPLLSPQLP FEELESQRGLPQLKSYLRQKLQALGLRWGPEGG *GVDVGFRMAPPWGVDDLHFGSLCFDFSLKFLS F

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTWWYRSWSCPLGD*TTCPWNSSL CPALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVVRSQDGWLGP*GLERQG SRVACSPEPQSPPPHRHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQVDGAAWGKGRGQPGTSRVEKPEGP*SAKAKE LSATIKLGPEAAEGFAQGGGTRLGQIRMRCRAL PATGVLSPQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHR*ARGRRQARGDGALAKAAGPAPLQTSC CAPAALVLCFP*GPRTPSCHTHQGPAHRARLPL APLLETLPRGKGGRVGEPWPPCPPCPEPRPSPCR VVLVLRERWHFCRDGRVLLGSRALRERHLGLMG YQLLPVSGPGLARGASSLPACG*PAALSSPHSY PSRNWSPREACPSSRAT*GRSSRPWACAGGLKGA EGLMWGSGWPPHGGWMICTLVPCVLISH*SSFP S

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGGTEVGPALWATELPAPGTAVY ALP*EDPGSGSRGGTPGYSQHLDVTVPTAVPAL QSPALCFFPWLHQLHQWYAARMAGWAPEGWRGRG AGWPAAQSPSPRLPTGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYRWMGQPGARGGASLGPAEWKSQRDHEAPKLRS SRPP*NWALKQQRALPRVVALDWDRLG*GAGPF LLLVSFLLSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTGEQGAGGRPGETEPWLRPPALLPSRLPA VRQQLWCCASREDPGPLPAIPTKVLPTGPGCL* RHYSRPCPEVKEAGWGSPGHLARHALSHVPPPA GWCWCCGNAGISAGTAGCCWARGP*GSGT*A*WA TSSCR*VALAWPVAHPHSQPAGNLPPSPLPTAT LRGTGVPERPAPAQELPEAEAPGPGPALGA*RG LRG*CGVQDGPPMGGG*FALWFPVF*FLIKVPFL P

Sequence FASTK.nAug05

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESWSPS PNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLG PSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGE LLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRP PPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHL AVLLGGTEVGPALWATELPAPGTAVYALP*EDP GSGSRGGTPGYSQHLDVTVPTAVPALQSPALCFF PWLHQLHQWHPSCSDCASLPLPAGHGRGAGAPR IPGSPPSPKAASAHLSPASHHRPCPLQVQSQGH SS*GVAPAAGGGEIPPGPDCASRLLHSSGAVLPV RTQDPFLPYPPRSCPQGQAASSATTRDPAQRVV LVLRERWHFCRDGRVLLGSRALRERHLGLMGYQ LLPLPFEELESQRGLPQLKSYLRQKLQALGLRWG PEGG*GVDVGFRMAPPWGVDDLHFGSLCFDFSL KFLSFPV

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHGLH HPTPCFESCSLLRPPLLGCLACC*SLQYSPAVWG PANGGTGLLEEAPVQVLCKDCSGFWNRRRALGS CCAGWARTPARCAPTTTRWRFVVWASSWGLGHG PLLWSRSHCRT*VSSSSETAPPLTFTPSTCVCTL QSYLVVQKLVLPFGRLNYLPLEQQFMPCLERIL AREAGVAPLATVNILMSLCQLRCLPFRALHFVF SPGFINYISGTPHALIVRRYLSLLDTAVELELPG YRGPRLPRRQQVPIFPQPLITDRARCKYSHKDI VAEGLRQLLGEEKYRQDLTVPPGYCTALVLCFP *GPRTPSCHTHQGPAHRARLPLAPLLETLPRGWC WCCGNAGISAGTAGCCWARGP*GSGT*A*WATS SCRYPSRNWSPREACPSSRAT*GRSSRPWACAG GLKGAEGLMWGSGWPPHGGWMICTLVPCVLISH* SSFPSP

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVMVSI TQLHASSPALCSDLPCSAVWPAADPSSTALLFG AQQMGGPACWRRPQCRSCARTAAASGTGEEPWGA AALAGPEPQQGARPPLLGGASSSGPALGVSATA PSCGAGHTAGLESAHHPKLPLL*HSHHPRVSAP CSLTWWYRSWSCPLGD*TTCPWNSSLCPALRGSW LGKQGWHPWLQSTS*CHCANCGACPSEPCTLFF PLASSTTSVAPLML*LCVATSPCWTRPWSWSSQ DTGVPAFPEGSKCPSFPSLSSPTVPAASTVTRT* *LRGCASCWGRRNTART*LCLQATAQLWCCASR EDPGPLPAIPTKVLPTGPGCL*RHYSRPCPEGG AGVAGTLAFLPGRPGAAGLEGPEGAAPRPDGLPA PAATLRGTGVPERPAPAQELPEAEAPGPGPALG A*RGLRG*CGVQDGPPMGGG*FALWFPVF*FLI KVPFLPR

Sequence FASTK.jAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGAGGAGGGGCTTGAGGTGGGCGCCTGGGGGGCTAGGTGTTAATGAAGAATAGGGAGAACAGTA GAGGACCACGGACAGAGACCCAGCCTCTCAGGCGTTTGCCCAGGAACGCTTGCTCCTGCTCCCGTCCTATGTCTGCGGCAGACATCATGAGTCGAACGCA CTCCTTCCGGCCTCGCTAGGGTGCCACCCCAGATTCCTGCTAATAGAGTGCCCTAGGAAGCCACCATAATTTGTCACAATTGGTATGAGATGAAAGCCTA TTTGCCATCTTTGTATGTACTCCCTACAAACCGTTTCGATACCTCATAGCCACATGATACTTTACATGTCTCTAGTGTTTGCACTTAAACTCTAGCACAC CTTCCCGCTCAGGAAACGGGAGAGGAGAAAGGTGGAGAATTCACCAAGTAACCCCATGATAAGAACAGCAGCACAGCTCTGTTACCAGGCTGCCTTTTAC CTCTCCCCCTCAGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAG CTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTC CTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGGTGAGCAAGGGGCAGGCGGCAGGCCCGGGGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTCCCCTCCAGACTTCCTGC TGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAG CGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCACGTCCCTCCCCCTGCAG GGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGC TACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGC GCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGTTTTGATTT CTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVRRGLRWAPGGLGVNEE*GEQ* RTTDRDPASQAFAQERLLLLPSYVCGRHHESNA LLPASLGCHPRFLLIECPRKPP*FVTIGMR*KP ICHLCMYSLQTVSIPHSHMILYMSLVFALKL*HT FPLRKRERRKVENSPSNPMIRTAAQLCYQAAFY LSPSGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYRWMGQPGARGGASLGPAEWKSQRDHEAPKLRS SRPP*NWALKQQRALPRVVALDWDRLG*GAGPF LLLVSFLLSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTGEQGAGGRPGETEPWLRPPALLPSRLPA VRQQLWCCASREDPGPLPAIPTKVLPTGPGCL* RHYSRPCPEVKEAGWGSPGHLARHALSHVPPPA GWCWCCGNAGISAGTAGCCWARGP*GSGT*A*WA TSSCRYPSRNWSPREACPSSRAT*GRSSRPWAC AGGLKGAEGLMWGSGWPPHGGWMICTLVPCVLI SH*SSFPSP

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTW*GGA*GGRLGG*VLMKNRENS RGPRTETQPLRRLPRNACSCSRPMSAADIMSRTH SFRPR*GATPDSC**SALGSHHNLSQLV*DESL FAIFVCTPYKPFRYLIAT*YFTCL*CLHLNSST PSRSGNGRGERWRIHQVTP**EQQHSSVTRLPFT SPPQAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STGGWGSLGQGEGPAWDQQSGKARGTMKRQS*G ALGHHKTGP*SSRGLCPGWWH*TGTD*DEVPGPS CYWCPFSSVTRT**LRGCASCWGRRNTART*LC LQATAQVSKGQAAGPGRRSPG*GRRPCSPPDFL LCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASS ATTRDPAQR*RRQGGGALATLPAMP*ATSLPLQ GGAGVAGTLAFLPGRPGAAGLEGPEGAAPRPDG LPAPAATLRGTGVPERPAPAQELPEAEAPGPGPA LGA*RGLRG*CGVQDGPPMGGG*FALWFPVF*F LIKVPFLPR

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGEEGLEVGAWGARC**RIGRTV EDHGQRPSLSGVCPGTLAPAPVLCLRQTS*VER TPSGLARVPPQIPANRVP*EATIICHNWYEMKAY LPSLYVLPTNRFDTS*PHDTLHVSSVCT*TLAH LPAQETGEEKGGEFTK*PHDKNSSTALLPGCLL PLPLRLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQVDGAAWGKGRGQPGTSRVEKPEGP*SAKAKE LSATIKLGPEAAEGFAQGGGTRLGQIRMRCRAL PATGVLSPQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHR*ARGRRQARGDGALAKAAGPAPLQTSC CAPAALVLCFP*GPRTPSCHTHQGPAHRARLPL APLLETLPRGKGGRVGEPWPPCPPCPEPRPSPCR VVLVLRERWHFCRDGRVLLGSRALRERHLGLMG YQLLPLPFEELESQRGLPQLKSYLRQKLQALGL RWGPEGG*GVDVGFRMAPPWGVDDLHFGSLCFDF SLKFLSFPV

Sequence FASTK.iAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGAGGAGGGGCTTGAGGTGGGCGCCTGGGGGGCTAGGTGTTAATGAAGAATAGGGAGAACAGTA GAGGACCACGGACAGAGACCCAGCCTCTCAGGCGTTTGCCCAGGAACGCTTGCTCCTGCTCCCGTCCTATGTCTGCGGCAGACATCATGAGTCGAACGCA CTCCTTCCGGCCTCGCTAGGGTGCCACCCCAGATTCCTGCTAATAGAGTGCCCTAGGAAGCCACCATAATTTGTCACAATTGGTATGAGATGAAAGCCTA TTTGCCATCTTTGTATGTACTCCCTACAAACCGTTTCGATACCTCATAGCCACATGATACTTTACATGTCTCTAGTGTTTGCACTTAAACTCTAGCACAC CTTCCCGCTCAGGAAACGGGAGAGGAGAAAGGTGGAGAATTCACCAAGTAACCCCATGATAAGAACAGCAGCACAGCTCTGTTACCAGGCTGCCTTTTAC CTCTCCCCCTCAGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAG CTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTC CTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTC CTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATG CCCTGAGCCACGTCCCTCCCCCTGCAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTG AGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGA GGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTT GCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVRRGLRWAPGGLGVNEE*GEQ* RTTDRDPASQAFAQERLLLLPSYVCGRHHESNA LLPASLGCHPRFLLIECPRKPP*FVTIGMR*KP ICHLCMYSLQTVSIPHSHMILYMSLVFALKL*HT FPLRKRERRKVENSPSNPMIRTAAQLCYQAAFY LSPSGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYRWMGQPGARGGASLGPAEWKSQRDHEAPKLRS SRPP*NWALKQQRALPRVVALDWDRLG*GAGPF LLLVSFLLSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRS CPQGQAASSATTRDPAQR*RRQGGGALATLPAM P*ATSLPLQGGAGVAGTLAFLPGRPGAAGLEGP EGAAPRPDGLPAPAATLRGTGVPERPAPAQELPE AEAPGPGPALGA*RGLRG*CGVQDGPPMGGG*F ALWFPVF*FLIKVPFLPR

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTW*GGA*GGRLGG*VLMKNRENS RGPRTETQPLRRLPRNACSCSRPMSAADIMSRTH SFRPR*GATPDSC**SALGSHHNLSQLV*DESL FAIFVCTPYKPFRYLIAT*YFTCL*CLHLNSST PSRSGNGRGERWRIHQVTP**EQQHSSVTRLPFT SPPQAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STGGWGSLGQGEGPAWDQQSGKARGTMKRQS*G ALGHHKTGP*SSRGLCPGWWH*TGTD*DEVPGPS CYWCPFSSVTRT**LRGCASCWGRRNTART*LC LQATAQTSCCAPAALVLCFP*GPRTPSCHTHQG PAHRARLPLAPLLETLPRGKGGRVGEPWPPCPPC PEPRPSPCRVVLVLRERWHFCRDGRVLLGSRAL RERHLGLMGYQLLPLPFEELESQRGLPQLKSYL RQKLQALGLRWGPEGG*GVDVGFRMAPPWGVDDL HFGSLCFDFSLKFLSFPV

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGEEGLEVGAWGARC**RIGRTV EDHGQRPSLSGVCPGTLAPAPVLCLRQTS*VER TPSGLARVPPQIPANRVP*EATIICHNWYEMKAY LPSLYVLPTNRFDTS*PHDTLHVSSVCT*TLAH LPAQETGEEKGGEFTK*PHDKNSSTALLPGCLL PLPLRLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQVDGAAWGKGRGQPGTSRVEKPEGP*SAKAKE LSATIKLGPEAAEGFAQGGGTRLGQIRMRCRAL PATGVLSPQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHRLPAVRQQLWCCASREDPGPLPAIPTKV LPTGPGCL*RHYSRPCPEVKEAGWGSPGHLARH ALSHVPPPAGWCWCCGNAGISAGTAGCCWARGP* GSGT*A*WATSSCRYPSRNWSPREACPSSRAT* GRSSRPWACAGGLKGAEGLMWGSGWPPHGGWMI CTLVPCVLISH*SSFPSP