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Sequences

Sequence FASTK.dAug05

AGTGCCTGCCGGGAGCCACGTCTCCGAAGACCGATAGCTGCTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG GCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGGTCTCCATC ACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGG CCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGAGCCCTGGGGAGC TGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCC CCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACCTT GCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGCCTCCAGGCTACTGCACAGG TGAGCAAGGGGCAGGCGGCAGGCCCGGGGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTCCCCTCCAGACTTCCTGCTGTGCGCCAGCAGCTCTGG TGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCT GCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCC TGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCT GGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGT TTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESWSPS PNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLG PSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGE LLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRP PPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHL AVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTG EQGAGGRPGETEPWLRPPALLPSRLPAVRQQLW CCASREDPGPLPAIPTKVLPTGPGCL*RHYSRP CPEGGAGVAGTLAFLPGRPGAAGLEGPEGAAPRP DGLPAPAATLRGTGVPERPAPAQELPEAEAPGP GPALGA*RGLRG*CGVQDGPPMGGG*FALWFPV F*FLIKVPFLPR

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHGLH HPTPCFESCSLLRPPLLGCLACC*SLQYSPAVWG PANGGTGLLEEAPVQVLCKDCSGFWNRRRALGS CCAGWARTPARCAPTTTRWRFVVWASSWGLGHG PLLWSRSHCRT*VSSSSETAPPLTFTPSTCVCTL QSYLAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STVTRT**LRGCASCWGRRNTART*LCLQATAQ VSKGQAAGPGRRSPG*GRRPCSPPDFLLCASSSG AVLPVRTQDPFLPYPPRSCPQGQAASSATTRDP AQRVVLVLRERWHFCRDGRVLLGSRALRERHLG LMGYQLLPLPFEELESQRGLPQLKSYLRQKLQAL GLRWGPEGG*GVDVGFRMAPPWGVDDLHFGSLC FDFSLKFLSFPV

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVMVSI TQLHASSPALCSDLPCSAVWPAADPSSTALLFG AQQMGGPACWRRPQCRSCARTAAASGTGEEPWGA AALAGPEPQQGARPPLLGGASSSGPALGVSATA PSCGAGHTAGLESAHHPKLPLL*HSHHPRVSAP CSLTWLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQSQGHSS*GVAPAAGGGEIPPGPDCASRLLHR *ARGRRQARGDGALAKAAGPAPLQTSCCAPAAL VLCFP*GPRTPSCHTHQGPAHRARLPLAPLLETL PRGWCWCCGNAGISAGTAGCCWARGP*GSGT*A *WATSSCRYPSRNWSPREACPSSRAT*GRSSRP WACAGGLKGAEGLMWGSGWPPHGGWMICTLVPCV LISH*SSFPSP

Sequence FASTK.cAug05

AGTGCCTGCCGGGAGCCACGTCTCCGAAGACCGATAGCTGCTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG GCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGG CGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGTGTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCC CGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTCGGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACT TAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGG CTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTG TGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTC GGTGGCGCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGA AACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAAC AGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGCCCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTT TCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAGGCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCC CGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCC TGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTAC AGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACC CCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGC AAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCAAGTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGA GGAGAAATACCGCCAGGACCTGACTGTGCCTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAG GACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTG GGGGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCACGTCCCTCCCCCTGCAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACG GCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAG AGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGG ATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESCRGG ACGEGCGRAAGAGVSGSGHGLLASLGRGGCWGP RLRRLRAIAKRCTRAPRAPCEVGAAFSFHG*GD LERRSHLSKVTPLGLHHPTPCFESCSLLRPPLLG CLACC*SLQYSPAVWGPANGGTGLLEEAPVQVL CKDCSGFWNRRRALGSCCAGWARTPARCAPTTT RWRFVVWASSWGLGHGPLLWSRSHCRT*VSSSSE TAPPLTFTPSTCVCTLQSYLAFHLMVPWCVPWN RSEGSASLRSHLPLCSPFSEVGKGWKLL*AAPV FCGIHGSI*SAAWQRQGQRN*LPT*WCSWPSTWP GTGCGSPSFWKPLPTSWWFRKRNSAARWYRSWS CPLGD*TTCPWNSSLCPALRGSWLGKQGWHPWL QSTS*CHCANCGACPSEPCTLFFPLASSTTSVAP LML*LCVATSPCWTRPWSWSSQDTGVPAFPEGS KCPSFPSLSSPTVPAASTVTRT**LRGCASCWG RRNTART*LCLQATAQTSCCAPAALVLCFP*GPR TPSCHTHQGPAHRARLPLAPLLETLPRGKGGRV GEPWPPCPPCPEPRPSPCRVVLVLRERWHFCRD GRVLLGSRALRERHLGLMGYQLLPLPFEELESQR GLPQLKSYLRQKLQALGLRWGPEGG*GVDVGFR MAPPWGVDDLHFGSLCFDFSLKFLSFPV

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHVGV APAGRAAGGQRELVLAAAATVSWRPWAGVGVGAP DFADSAPSQSGALGLHARPARWALRFHFTGEET *RDEATCPRSRRWVSITQLHASSPALCSDLPCS AVWPAADPSSTALLFGAQQMGGPACWRRPQCRSC ARTAAASGTGEEPWGAAALAGPEPQQGARPPLL GGASSSGPALGVSATAPSCGAGHTAGLESAHHP KLPLL*HSHHPRVSAPCSLTWLSI*WSPGVCPGT GAKAPPPSEATSPFAAPSPRWARVGSCSKLPPF SAVSTAASDQQPGRGKARGTDSPRDGAPGPAPG PAPVAGAPASGSHCPLPGGSGNATQQQGGTEVGP ALWATELPAPGTAVYALP*EDPGSGSRGGTPGY SQHLDVTVPTAVPALQSPALCFFPWLHQLHQWH PSCSDCASLPLPAGHGRGAGAPRIPGSPPSPKAA SAHLSPASHHRPCPLQVQSQGHSS*GVAPAAGG GEIPPGPDCASRLLHRLPAVRQQLWCCASREDP GPLPAIPTKVLPTGPGCL*RHYSRPCPEVKEAGW GSPGHLARHALSHVPPPAGWCWCCGNAGISAGT AGCCWARGP*GSGT*A*WATSSCRYPSRNWSPR EACPSSRAT*GRSSRPWACAGGLKGAEGLMWGSG WPPHGGWMICTLVPCVLISH*SSFPSP

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVM*GW RLRGGLREGSGSWC*RQRPRSPGVPGPGWVLGP PTSPTPRHRKAVHSGSTRALRGGRCVFISRVRRL RETKPLVQGHAAGSPSPNSMLRVLLSAQTSPAR LSGLLLIPPVQPCCLGPSKWGDRPVGGGPSAGP VQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYS VALRRLGQLLGSRPRPPPVEQVTLQDLSQLIIR NCPSFDIHTIHVCLHLAVLLGFPSDGPLVCALE QERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRF LRYPRQHLISSLAEARPEELTPHVMVLLAQHLA RHRLREPQLLEAIAHFLVVQETQLSSKVVQKLV LPFGRLNYLPLEQQFMPCLERILAREAGVAPLAT VNILMSLCQLRCLPFRALHFVFSPGFINYISGT PHALIVRRYLSLLDTAVELELPGYRGPRLPRRQ QVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGE EKYRQDLTVPPGYCTDFLLCASSSGAVLPVRTQ DPFLPYPPRSCPQGQAASSATTRDPAQR*RRQG GGALATLPAMP*ATSLPLQGGAGVAGTLAFLPGR PGAAGLEGPEGAAPRPDGLPAPAATLRGTGVPE RPAPAQELPEAEAPGPGPALGA*RGLRG*CGVQ DGPPMGGG*FALWFPVF*FLIKVPFLPR

Sequence FASTK.aAug05

AGTGCCTGCCGGGAGCCACGTCTCCGAAGACCGATAGCTGCTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG GCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGGTCTCCATC ACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGG CCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGAGCCCTGGGGAGC TGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCC CCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACCTT GCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGCCTCCAGGCTACTGCACAGA CTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCT GCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGA GGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAG CTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTG GATGATTTGCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESWSPS PNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLG PSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGE LLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRP PPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHL AVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTD FLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQA ASSATTRDPAQRVVLVLRERWHFCRDGRVLLGS RALRERHLGLMGYQLLPLPFEELESQRGLPQLKS YLRQKLQALGLRWGPEGG*GVDVGFRMAPPWGV DDLHFGSLCFDFSLKFLSFPV

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHGLH HPTPCFESCSLLRPPLLGCLACC*SLQYSPAVWG PANGGTGLLEEAPVQVLCKDCSGFWNRRRALGS CCAGWARTPARCAPTTTRWRFVVWASSWGLGHG PLLWSRSHCRT*VSSSSETAPPLTFTPSTCVCTL QSYLAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STVTRT**LRGCASCWGRRNTART*LCLQATAQ TSCCAPAALVLCFP*GPRTPSCHTHQGPAHRARL PLAPLLETLPRGWCWCCGNAGISAGTAGCCWAR GP*GSGT*A*WATSSCRYPSRNWSPREACPSSR AT*GRSSRPWACAGGLKGAEGLMWGSGWPPHGGW MICTLVPCVLISH*SSFPSP

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVMVSI TQLHASSPALCSDLPCSAVWPAADPSSTALLFG AQQMGGPACWRRPQCRSCARTAAASGTGEEPWGA AALAGPEPQQGARPPLLGGASSSGPALGVSATA PSCGAGHTAGLESAHHPKLPLL*HSHHPRVSAP CSLTWLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQSQGHSS*GVAPAAGGGEIPPGPDCASRLLHR LPAVRQQLWCCASREDPGPLPAIPTKVLPTGPG CL*RHYSRPCPEGGAGVAGTLAFLPGRPGAAGLE GPEGAAPRPDGLPAPAATLRGTGVPERPAPAQE LPEAEAPGPGPALGA*RGLRG*CGVQDGPPMGG G*FALWFPVF*FLIKVPFLPR

Sequence FASTK.bAug05

AGTGCCTGCCGGGAGCCACGTCTCCGAAGACCGATAGCTGCTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTG GCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGGTCTCCATC ACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGG CCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGAGCCCTGGGGAGC TGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCC CCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACCTT GCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGAGGCAAGGCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCT GGAAGCCATTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCC CTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAAC TGCGGTGCCTGCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCT CTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATC ACCGACCGTGCCCGCTGCAAGTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTC CTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGC CGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAG GCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGAT GGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

SACREPRLRRPIAASGLASGRLSRGCWEDGGLG G*PMRRPRGEPGPRAPRPTEGATCAGPGESWSPS PNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLG PSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGE LLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRP PPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHL AVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLREARPEELTPHVMVLLAQHLARHRLREPQLL EAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLP LEQQFMPCLERILAREAGVAPLATVNILMSLCQ LRCLPFRALHFVFSPGFINYISGTPHALIVRRYL SLLDTAVELELPGYRGPRLPRRQQVPIFPQPLI TDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRS CPQGQAASSATTRDPAQRVVLVLRERWHFCRDG RVLLGSRALRERHLGLMGYQLLPLPFEELESQR GLPQLKSYLRQKLQALGLRWGPEGG*GVDVGFRM APPWGVDDLHFGSLCFDFSLKFLSFPV

Frame 1

VPAGSHVSEDR*LLRDWRPGGYLGAAGKMADSV ASR*GGRGGNPAPGPRDRLRERPAQGPGSHGLH HPTPCFESCSLLRPPLLGCLACC*SLQYSPAVWG PANGGTGLLEEAPVQVLCKDCSGFWNRRRALGS CCAGWARTPARCAPTTTRWRFVVWASSWGLGHG PLLWSRSHCRT*VSSSSETAPPLTFTPSTCVCTL QSYLAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSERQGQRN*LPT*WCSWPSTWPGTGCGSPSF WKPLPTSWWFRKRNSAARWYRSWSCPLGD*TTCP WNSSLCPALRGSWLGKQGWHPWLQSTS*CHCAN CGACPSEPCTLFFPLASSTTSVAPLML*LCVAT SPCWTRPWSWSSQDTGVPAFPEGSKCPSFPSLSS PTVPAASTVTRT**LRGCASCWGRRNTART*LC LQATAQTSCCAPAALVLCFP*GPRTPSCHTHQG PAHRARLPLAPLLETLPRGWCWCCGNAGISAGTA GCCWARGP*GSGT*A*WATSSCRYPSRNWSPRE ACPSSRAT*GRSSRPWACAGGLKGAEGLMWGSG WPPHGGWMICTLVPCVLISH*SSFPSP

Frame 2

CLPGATSPKTDSCFGIGVRAAI*GLLGRWRTRW LADEEAAGGTRPPGPETD*GSDLRRARGVMVSI TQLHASSPALCSDLPCSAVWPAADPSSTALLFG AQQMGGPACWRRPQCRSCARTAAASGTGEEPWGA AALAGPEPQQGARPPLLGGASSSGPALGVSATA PSCGAGHTAGLESAHHPKLPLL*HSHHPRVSAP CSLTWLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRGKARGTDSPRDGAPGPAPGPAPVAGAPAS GSHCPLPGGSGNATQQQGGTEVGPALWATELPA PGTAVYALP*EDPGSGSRGGTPGYSQHLDVTVPT AVPALQSPALCFFPWLHQLHQWHPSCSDCASLP LPAGHGRGAGAPRIPGSPPSPKAASAHLSPASH HRPCPLQVQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHRLPAVRQQLWCCASREDPGPLPAIPTKV LPTGPGCL*RHYSRPCPEGGAGVAGTLAFLPGR PGAAGLEGPEGAAPRPDGLPAPAATLRGTGVPER PAPAQELPEAEAPGPGPALGA*RGLRG*CGVQD GPPMGGG*FALWFPVF*FLIKVPFLPR

Sequence FASTK.mAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGAGGAGGGGCTTGAGGTGGGCGCCTGGGGGGCTAGGTGTTAATGAAGAATAGGGAGAACAGTA GAGGACCACGGACAGAGACCCAGCCTCTCAGGCGTTTGCCCAGGAACGCTTGCTCCTGCTCCCGTCCTATGTCTGCGGCAGACATCATGAGTCGAACGCA CTCCTTCCGGCCTCGCTAGGGTGCCACCCCAGATTCCTGCTAATAGAGTGCCCTAGGAAGCCACCATAATTTGTCACAATTGGTATGAGATGAAAGCCTA TTTGCCATCTTTGTATGTACTCCCTACAAACCGTTTCGATACCTCATAGCCACATGATACTTTACATGTCTCTAGTGTTTGCACTTAAACTCTAGCACAC CTTCCCGCTCAGGAAACGGGAGAGGAGAAAGGTGGAGAATTCACCAAGTAACCCCATGATAAGAACAGCAGCACAGCTCTGTTACCAGGCTGCCTTTTAC CTCTCCCCCTCAGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGAGGCAAGGCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCT GGAAGCCATTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCC CTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAAC TGCGGTGCCTGCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGTACGCAGCCAGGATGGCTGGCTGGGCCCCTGAG GGCTGGAGAGGCAGGGGAGCAGGGTGGCCTGCAGCCCAGAGCCCCAGTCCCCGCCTCCCCACAGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCT CCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCAC CGACCGTGCCCGCTGCAAGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAA GCGCCAAAGCTAAGGAGCTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGAT GAGGTGCCGGGCCCTTCCTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCG CCAGGACCTGACTGTGCCTCCAGGCTACTGCACAGGTGAGCAAGGGGCAGGCGGCAGGCCCGGGGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTC CCCTCCAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAG GGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCA CGTCCCTCCCCCTGCAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGC ACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCT CCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGT TCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVRRGLRWAPGGLGVNEE*GEQ* RTTDRDPASQAFAQERLLLLPSYVCGRHHESNA LLPASLGCHPRFLLIECPRKPP*FVTIGMR*KP ICHLCMYSLQTVSIPHSHMILYMSLVFALKL*HT FPLRKRERRKVENSPSNPMIRTAAQLCYQAAFY LSPSGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLREARPEELTPHVMVLLAQHLARHRLREPQLL EAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLP LEQQFMPCLERILAREAGVAPLATVNILMSLCQ LRCLPFRALHFVFSPGFINYISGTQPGWLAGPLR AGEAGEQGGLQPRAPVPASPQAPLML*LCVATS PCWTRPWSWSSQDTGVPAFPEGSKCPSFPSLSS PTVPAASTGGWGSLGQGEGPAWDQQSGKARGTMK RQS*GALGHHKTGP*SSRGLCPGWWH*TGTD*D EVPGPSCYWCPFSSVTRT**LRGCASCWGRRNT ART*LCLQATAQVSKGQAAGPGRRSPG*GRRPCS PPDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQ GQAASSATTRDPAQR*RRQGGGALATLPAMP*A TSLPLQGGAGVAGTLAFLPGRPGAAGLEGPEGAA PRPDGLPAPAATLRGTGVPERPAPAQELPEAEA PGPGPALGA*RGLRG*CGVQDGPPMGGG*FALW FPVF*FLIKVPFLPR

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTW*GGA*GGRLGG*VLMKNRENS RGPRTETQPLRRLPRNACSCSRPMSAADIMSRTH SFRPR*GATPDSC**SALGSHHNLSQLV*DESL FAIFVCTPYKPFRYLIAT*YFTCL*CLHLNSST PSRSGNGRGERWRIHQVTP**EQQHSSVTRLPFT SPPQAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSERQGQRN*LPT*WCSWPSTWPGTGCGSPSF WKPLPTSWWFRKRNSAARWYRSWSCPLGD*TTCP WNSSLCPALRGSWLGKQGWHPWLQSTS*CHCAN CGACPSEPCTLFFPLASSTTSVVRSQDGWLGP* GLERQGSRVACSPEPQSPPPHRHPSCSDCASLPL PAGHGRGAGAPRIPGSPPSPKAASAHLSPASHH RPCPLQVQVDGAAWGKGRGQPGTSRVEKPEGP* SAKAKELSATIKLGPEAAEGFAQGGGTRLGQIRM RCRALPATGVLSPQSQGHSS*GVAPAAGGGEIP PGPDCASRLLHR*ARGRRQARGDGALAKAAGPA PLQTSCCAPAALVLCFP*GPRTPSCHTHQGPAHR ARLPLAPLLETLPRGKGGRVGEPWPPCPPCPEP RPSPCRVVLVLRERWHFCRDGRVLLGSRALRER HLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKL QALGLRWGPEGG*GVDVGFRMAPPWGVDDLHFG SLCFDFSLKFLSFPV

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGEEGLEVGAWGARC**RIGRTV EDHGQRPSLSGVCPGTLAPAPVLCLRQTS*VER TPSGLARVPPQIPANRVP*EATIICHNWYEMKAY LPSLYVLPTNRFDTS*PHDTLHVSSVCT*TLAH LPAQETGEEKGGEFTK*PHDKNSSTALLPGCLL PLPLRLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRGKARGTDSPRDGAPGPAPGPAPVAGAPAS GSHCPLPGGSGNATQQQGGTEVGPALWATELPA PGTAVYALP*EDPGSGSRGGTPGYSQHLDVTVPT AVPALQSPALCFFPWLHQLHQWYAARMAGWAPE GWRGRGAGWPAAQSPSPRLPTGTPHALIVRRYL SLLDTAVELELPGYRGPRLPRRQQVPIFPQPLIT DRARCKYRWMGQPGARGGASLGPAEWKSQRDHE APKLRSSRPP*NWALKQQRALPRVVALDWDRLG *GAGPFLLLVSFLLSHKDIVAEGLRQLLGEEKYR QDLTVPPGYCTGEQGAGGRPGETEPWLRPPALL PSRLPAVRQQLWCCASREDPGPLPAIPTKVLPT GPGCL*RHYSRPCPEVKEAGWGSPGHLARHALSH VPPPAGWCWCCGNAGISAGTAGCCWARGP*GSG T*A*WATSSCRYPSRNWSPREACPSSRAT*GRS SRPWACAGGLKGAEGLMWGSGWPPHGGWMICTLV PCVLISH*SSFPSP

Sequence FASTK.jAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGAGGAGGGGCTTGAGGTGGGCGCCTGGGGGGCTAGGTGTTAATGAAGAATAGGGAGAACAGTA GAGGACCACGGACAGAGACCCAGCCTCTCAGGCGTTTGCCCAGGAACGCTTGCTCCTGCTCCCGTCCTATGTCTGCGGCAGACATCATGAGTCGAACGCA CTCCTTCCGGCCTCGCTAGGGTGCCACCCCAGATTCCTGCTAATAGAGTGCCCTAGGAAGCCACCATAATTTGTCACAATTGGTATGAGATGAAAGCCTA TTTGCCATCTTTGTATGTACTCCCTACAAACCGTTTCGATACCTCATAGCCACATGATACTTTACATGTCTCTAGTGTTTGCACTTAAACTCTAGCACAC CTTCCCGCTCAGGAAACGGGAGAGGAGAAAGGTGGAGAATTCACCAAGTAACCCCATGATAAGAACAGCAGCACAGCTCTGTTACCAGGCTGCCTTTTAC CTCTCCCCCTCAGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAG CTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTC CTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGGTGAGCAAGGGGCAGGCGGCAGGCCCGGGGAGACGGAGCCCTGGCTAAGGCCGCCGGCCCTGCTCCCCTCCAGACTTCCTGC TGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAG CGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATGCCCTGAGCCACGTCCCTCCCCCTGCAG GGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGC TACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCTCCAGGCCCTGGGCCTGC GCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTTGCACTTTGGTTCCCTGTGTTTTGATTT CTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVRRGLRWAPGGLGVNEE*GEQ* RTTDRDPASQAFAQERLLLLPSYVCGRHHESNA LLPASLGCHPRFLLIECPRKPP*FVTIGMR*KP ICHLCMYSLQTVSIPHSHMILYMSLVFALKL*HT FPLRKRERRKVENSPSNPMIRTAAQLCYQAAFY LSPSGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYRWMGQPGARGGASLGPAEWKSQRDHEAPKLRS SRPP*NWALKQQRALPRVVALDWDRLG*GAGPF LLLVSFLLSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTGEQGAGGRPGETEPWLRPPALLPSRLPA VRQQLWCCASREDPGPLPAIPTKVLPTGPGCL* RHYSRPCPEVKEAGWGSPGHLARHALSHVPPPA GWCWCCGNAGISAGTAGCCWARGP*GSGT*A*WA TSSCRYPSRNWSPREACPSSRAT*GRSSRPWAC AGGLKGAEGLMWGSGWPPHGGWMICTLVPCVLI SH*SSFPSP

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTW*GGA*GGRLGG*VLMKNRENS RGPRTETQPLRRLPRNACSCSRPMSAADIMSRTH SFRPR*GATPDSC**SALGSHHNLSQLV*DESL FAIFVCTPYKPFRYLIAT*YFTCL*CLHLNSST PSRSGNGRGERWRIHQVTP**EQQHSSVTRLPFT SPPQAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STGGWGSLGQGEGPAWDQQSGKARGTMKRQS*G ALGHHKTGP*SSRGLCPGWWH*TGTD*DEVPGPS CYWCPFSSVTRT**LRGCASCWGRRNTART*LC LQATAQVSKGQAAGPGRRSPG*GRRPCSPPDFL LCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASS ATTRDPAQR*RRQGGGALATLPAMP*ATSLPLQ GGAGVAGTLAFLPGRPGAAGLEGPEGAAPRPDG LPAPAATLRGTGVPERPAPAQELPEAEAPGPGPA LGA*RGLRG*CGVQDGPPMGGG*FALWFPVF*F LIKVPFLPR

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGEEGLEVGAWGARC**RIGRTV EDHGQRPSLSGVCPGTLAPAPVLCLRQTS*VER TPSGLARVPPQIPANRVP*EATIICHNWYEMKAY LPSLYVLPTNRFDTS*PHDTLHVSSVCT*TLAH LPAQETGEEKGGEFTK*PHDKNSSTALLPGCLL PLPLRLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQVDGAAWGKGRGQPGTSRVEKPEGP*SAKAKE LSATIKLGPEAAEGFAQGGGTRLGQIRMRCRAL PATGVLSPQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHR*ARGRRQARGDGALAKAAGPAPLQTSC CAPAALVLCFP*GPRTPSCHTHQGPAHRARLPL APLLETLPRGKGGRVGEPWPPCPPCPEPRPSPCR VVLVLRERWHFCRDGRVLLGSRALRERHLGLMG YQLLPLPFEELESQRGLPQLKSYLRQKLQALGL RWGPEGG*GVDVGFRMAPPWGVDDLHFGSLCFDF SLKFLSFPV

Sequence FASTK.iAug05

CTTCGGGATTGGCGTCCGGGCGGCTATCTAGGGGCTGCTGGGAAGATGGCGGACTCGGTGGCTAGCCGATGAGGAGGCCGCGGGGGGAACCCGGCCCCCG GGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGCCCGGGGAGTCATGTAGGGGTGGCGCCTGCGGGGAGGGCTGCGGGAGGGCAGCGGGAGCTGGT GTTAGCGGCAGCGGCCACGGTCTCCTGGCGTCCCTGGGCCGGGGTGGGTGTTGGGGCCCCCGACTTCGCCGACTCCGCGCCATCGCAAAGCGGTGCACTC GGGCTCCACGCGCGCCCTGCGAGGTGGGCGCTGCGTTTTCATTTCACGGGTGAGGAGACTTAGAGAGACGAAGCCACTTGTCCAAGGTCACGCCGCTGGT GAGTGGGAGCGCCCAGCATGGAACCCAGCACCGTCCAGCCGCGGAGCCCGCGTTCCACCCTCTGTGCCGCCGCCGCCTCCTGTGGGAGAGGGAGGTGGTC GGGAGAGTGCACGCCGGTGCCGCCTGGGCTCCAGACTGGGCGCGACCACTAACCCGGTTAATGACCTCGGGCTTAACTTAACCCCTTCGCTGCTCTGGGC CTGCGTTTCTCCACCGGTGAAATGAGGTAATGCCAGCCCTGCCTACTCTTCATGGGCGTGTAAGGAGCATCAGAGATGGTGCATGTGGAATTGCTTTGCA AGTAGTGCTGCGCTGAGCGCACTGAGGATTATGATTGCTGTTTCAAAGGCGTGGAAGGAGGCAGAGGGGCGGGGTGGGGAAGAGGCCCAGAGCAGTCACA AAGGCTTGCTCTGACCACAGGCCCCCTAAATGAGGAGTGAGGTAAGCAGTAGGTAGCAGGTCTTTCTCCCCTTTGGAGAAAACCTCACCCCCTTTTTTGG GCTGCCCCTCAGGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGCTCGGCTGTCTGGCCTGCTGCTGATCCCTCC AGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGACCGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAA CAGGCGAAGAGCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGCGCTTCGTCGTCTGGGCCAGC TCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAGGACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACAC CATCCACGTGTGTCTGCACCTTGCAGTCTTACTTGGTGAGGAGGGGCTTGAGGTGGGCGCCTGGGGGGCTAGGTGTTAATGAAGAATAGGGAGAACAGTA GAGGACCACGGACAGAGACCCAGCCTCTCAGGCGTTTGCCCAGGAACGCTTGCTCCTGCTCCCGTCCTATGTCTGCGGCAGACATCATGAGTCGAACGCA CTCCTTCCGGCCTCGCTAGGGTGCCACCCCAGATTCCTGCTAATAGAGTGCCCTAGGAAGCCACCATAATTTGTCACAATTGGTATGAGATGAAAGCCTA TTTGCCATCTTTGTATGTACTCCCTACAAACCGTTTCGATACCTCATAGCCACATGATACTTTACATGTCTCTAGTGTTTGCACTTAAACTCTAGCACAC CTTCCCGCTCAGGAAACGGGAGAGGAGAAAGGTGGAGAATTCACCAAGTAACCCCATGATAAGAACAGCAGCACAGCTCTGTTACCAGGCTGCCTTTTAC CTCTCCCCCTCAGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCGCCTCCCTCCGAAGCCACCTCCCCCTTTGCAGC CCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTAAGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAG GCCAGAGGAACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCTTCTGGAAGCCATTGCCCACTTC CTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTGGTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGC CCTGCCTTGAGAGGATCCTGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTGCCTGCCCTTCAG AGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCATGCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCC GTGGAGCTGGAGCTCCCAGGATACCGGGGTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTGCA AGTACAGGTGGATGGGGCAGCCTGGGGCAAGGGGAGGGGCCAGCCTGGGACCAGCAGAGTGGAAAAGCCAGAGGGACCATGAAGCGCCAAAGCTAAGGAG CTCTCGGCCACCATAAAACTGGGCCCTGAAGCAGCAGAGGGCTTTGCCCAGGGTGGTGGCACTAGACTGGGACAGATTAGGATGAGGTGCCGGGCCCTTC CTGCTACTGGTGTCCTTTCTCCTCAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAGGACCTGACTGTGC CTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTCCCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTC CTGCCCACAGGGCCAGGCTGCCTCTAGCGCCACTACTCGAGACCCTGCCCAGAGGTAAAGGAGGCAGGGTGGGGGAGCCCTGGCCACCTTGCCCGCCATG CCCTGAGCCACGTCCCTCCCCCTGCAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGACGGCCGGGTGCTGCTGGGCTCGAGGGCCCTG AGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGCCGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGA GGCAGAAGCTCCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGCTGAGGGGTTGATGTGGGGTTCAGGATGGCCCCCCCATGGGGGGTGGATGATTT GCACTTTGGTTCCCTGTGTTTTGATTTCTCATTAAAGTTCCTTTCCTTCCCCGTT

FAST_HUMAN-5 (forward primer)
FAST_HUMAN-6 (reverse primer)
Intron retention

Frame 0

LRDWRPGGYLGAAGKMADSVASR*GGRGGNPAP GPRDRLRERPAQGPGSHVGVAPAGRAAGGQRELV LAAAATVSWRPWAGVGVGAPDFADSAPSQSGAL GLHARPARWALRFHFTGEET*RDEATCPRSRRW *VGAPSMEPSTVQPRSPRSTLCAAAASCGRGRWS GECTPVPPGLQTGRDH*PG**PRA*LNPFAALG LRFSTGEMR*CQPCLLFMGV*GASEMVHVELLC K*CCAERTEDYDCCFKGVEGGRGAGWGRGPEQSQ RLALTTGPLNEE*GKQ*VAGLSPLWRKPHPLFW AAPQGLHHPTPCFESCSLLRPPLLGCLACC*SL QYSPAVWGPANGGTGLLEEAPVQVLCKDCSGFWN RRRALGSCCAGWARTPARCAPTTTRWRFVVWAS SWGLGHGPLLWSRSHCRT*VSSSSETAPPLTFT PSTCVCTLQSYLVRRGLRWAPGGLGVNEE*GEQ* RTTDRDPASQAFAQERLLLLPSYVCGRHHESNA LLPASLGCHPRFLLIECPRKPP*FVTIGMR*KP ICHLCMYSLQTVSIPHSHMILYMSLVFALKL*HT FPLRKRERRKVENSPSNPMIRTAAQLCYQAAFY LSPSGFPSDGPLVCALEQERRLRLPPKPPPPLQ PLLRGGQGLEAALSCPRFLRYPRQHLISSLAEAR PEELTPHVMVLLAQHLARHRLREPQLLEAIAHF LVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFM PCLERILAREAGVAPLATVNILMSLCQLRCLPFR ALHFVFSPGFINYISGTPHALIVRRYLSLLDTA VELELPGYRGPRLPRRQQVPIFPQPLITDRARC KYRWMGQPGARGGASLGPAEWKSQRDHEAPKLRS SRPP*NWALKQQRALPRVVALDWDRLG*GAGPF LLLVSFLLSHKDIVAEGLRQLLGEEKYRQDLTV PPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRS CPQGQAASSATTRDPAQR*RRQGGGALATLPAM P*ATSLPLQGGAGVAGTLAFLPGRPGAAGLEGP EGAAPRPDGLPAPAATLRGTGVPERPAPAQELPE AEAPGPGPALGA*RGLRG*CGVQDGPPMGGG*F ALWFPVF*FLIKVPFLPR

Frame 1

FGIGVRAAI*GLLGRWRTRWLADEEAAGGTRPP GPETD*GSDLRRARGVM*GWRLRGGLREGSGSW C*RQRPRSPGVPGPGWVLGPPTSPTPRHRKAVHS GSTRALRGGRCVFISRVRRLRETKPLVQGHAAG EWERPAWNPAPSSRGARVPPSVPPPPPVGEGGG RESARRCRLGSRLGATTNPVNDLGLNLTPSLLWA CVSPPVK*GNASPAYSSWACKEHQRWCMWNCFA SSAALSALRIMIAVSKAWKEAEGRGGEEAQSSH KGLL*PQAP*MRSEVSSR*QVFLPFGENLTPFFG LPLRVSITQLHASSPALCSDLPCSAVWPAADPS STALLFGAQQMGGPACWRRPQCRSCARTAAASG TGEEPWGAAALAGPEPQQGARPPLLGGASSSGPA LGVSATAPSCGAGHTAGLESAHHPKLPLL*HSH HPRVSAPCSLTW*GGA*GGRLGG*VLMKNRENS RGPRTETQPLRRLPRNACSCSRPMSAADIMSRTH SFRPR*GATPDSC**SALGSHHNLSQLV*DESL FAIFVCTPYKPFRYLIAT*YFTCL*CLHLNSST PSRSGNGRGERWRIHQVTP**EQQHSSVTRLPFT SPPQAFHLMVPWCVPWNRSEGSASLRSHLPLCS PFSEVGKGWKLL*AAPVFCGIHGSI*SAAWQRQ GQRN*LPT*WCSWPSTWPGTGCGSPSFWKPLPTS WWFRKRNSAARWYRSWSCPLGD*TTCPWNSSLC PALRGSWLGKQGWHPWLQSTS*CHCANCGACPS EPCTLFFPLASSTTSVAPLML*LCVATSPCWTRP WSWSSQDTGVPAFPEGSKCPSFPSLSSPTVPAA STGGWGSLGQGEGPAWDQQSGKARGTMKRQS*G ALGHHKTGP*SSRGLCPGWWH*TGTD*DEVPGPS CYWCPFSSVTRT**LRGCASCWGRRNTART*LC LQATAQTSCCAPAALVLCFP*GPRTPSCHTHQG PAHRARLPLAPLLETLPRGKGGRVGEPWPPCPPC PEPRPSPCRVVLVLRERWHFCRDGRVLLGSRAL RERHLGLMGYQLLPLPFEELESQRGLPQLKSYL RQKLQALGLRWGPEGG*GVDVGFRMAPPWGVDDL HFGSLCFDFSLKFLSFPV

Frame 2

SGLASGRLSRGCWEDGGLGG*PMRRPRGEPGPR APRPTEGATCAGPGESCRGGACGEGCGRAAGAG VSGSGHGLLASLGRGGCWGPRLRRLRAIAKRCT RAPRAPCEVGAAFSFHG*GDLERRSHLSKVTPLV SGSAQHGTQHRPAAEPAFHPLCRRRLLWEREVV GRVHAGAAWAPDWARPLTRLMTSGLT*PLRCSG PAFLHR*NEVMPALPTLHGRVRSIRDGACGIALQ VVLR*AH*GL*LLFQRRGRRQRGGVGKRPRAVT KACSDHRPPK*GVR*AVGSRSFSPLEKTSPPFL GCPSGSPSPNSMLRVLLSAQTSPARLSGLLLIPP VQPCCLGPSKWGDRPVGGGPSAGPVQGLQRLLE QAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQ LLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHT IHVCLHLAVLLGEEGLEVGAWGARC**RIGRTV EDHGQRPSLSGVCPGTLAPAPVLCLRQTS*VER TPSGLARVPPQIPANRVP*EATIICHNWYEMKAY LPSLYVLPTNRFDTS*PHDTLHVSSVCT*TLAH LPAQETGEEKGGEFTK*PHDKNSSTALLPGCLL PLPLRLSI*WSPGVCPGTGAKAPPPSEATSPFAA PSPRWARVGSCSKLPPFSAVSTAASDQQPGRGK ARGTDSPRDGAPGPAPGPAPVAGAPASGSHCPL PGGSGNATQQQGGTEVGPALWATELPAPGTAVYA LP*EDPGSGSRGGTPGYSQHLDVTVPTAVPALQ SPALCFFPWLHQLHQWHPSCSDCASLPLPAGHG RGAGAPRIPGSPPSPKAASAHLSPASHHRPCPLQ VQVDGAAWGKGRGQPGTSRVEKPEGP*SAKAKE LSATIKLGPEAAEGFAQGGGTRLGQIRMRCRAL PATGVLSPQSQGHSS*GVAPAAGGGEIPPGPDCA SRLLHRLPAVRQQLWCCASREDPGPLPAIPTKV LPTGPGCL*RHYSRPCPEVKEAGWGSPGHLARH ALSHVPPPAGWCWCCGNAGISAGTAGCCWARGP* GSGT*A*WATSSCRYPSRNWSPREACPSSRAT* GRSSRPWACAGGLKGAEGLMWGSGWPPHGGWMI CTLVPCVLISH*SSFPSP