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Sequences

Sequence AXIN1.bAug05

CTCGGGAGGCGATCGTGGCCGCTGCCGCCGCCAGAGCCGGCCGGAGTCGGCCCGGAGCCGCGTCCACGCGGAGCGGGCGGGTGGGAGCTGGCGGCTGGGC CCCTGCGGCGCCCCCGCGGCCTGCCCCGCGTCCGCCTCCGGGCCGCCGAGCCGCAGCCGCCGAGATGGGGGCCGCCCCGGGCCGCGCCCCCGCCGGGTCC CGCCCGCCGCGCTGCCGCTGAGCGCATGGGCCCGGACCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGTCCCGCCACCACCGCGCGCGGGACAGATTG ATTCACTTTGGAGCTGTAAGTACTGATGTATTAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTTCCCCT TGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAG TTTCTGCTCCGGGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCC TCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGG GCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGC CATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTG ATCGATCCTGCCATGTTTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAAT ATACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGA AGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCT GCCCCGAGGGTCTCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCAATGCCGGCT ATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGAT CCCCCCATACAGGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCACGTACCGG GTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGG AGGAGCGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGC TTGGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCCTGAGAGCATCCTGGACGAGCACGTACAG CGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCG CCTCGGGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCAC AGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGA GGCTACTCAGAGAGTGTTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTG AGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGAT CAGCAGGCACCGCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGC CCTCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGG CGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCC GTGTGACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAG CTGCTGACCAAAAAGGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCG TCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACTGATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTTGGCGGGCA CGGGTGTCACGGCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCCATCCCTGTGGTCTACCCGTGTCTAGAGGC AGGTAGGGGGTCCCTCCAAGTGGTCCACAAGCTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGCCCAGCCCAA GTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCCCCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTCCCTCTGGG ATTTGCCCACGTACCCAGGTCTGGGTGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATTGTACGCGCCACCGAGTGCCTTCAACACAG CTTGTCTCTTGCCTGCCACTGTGTGAATCGGCGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTGAGGGCCCGT GTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCGAGTCTGTTTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTT GGATAATAATGAAATAAAACTGTTTTTGAACCTGC

AXIN1-3 (forward primer)
AXIN1-4 (reverse primer)
Intron retention

Frame 0

LGRRSWPLPPPEPAGVGPEPRPRGAGGWELAAG PLRRPRGLPRVRLRAAEPQPPRWGPPRAAPPPGP ARRAAAERMGPDRAAPLREPGPGSRHHRARDRL IHFGAVSTDVLGCSAHCSLTQSPKMNIQEQGFP LDLGASFTEDAPRPPVPGEEGELVSTDPRPASYS FCSGKGVGIKGETSTATPRRSDLDLGYEPEGSA SPTPPYLKWAESLHSLLDDQDGISLFRTFLKQE GCADLLDFWFACTGFRKLEPCDSNEEKRLKLARA IYRKYILDNNGIVSRQTKPATKSFIKGCIMKQL IDPAMFDQAQTEIQATMEENTYPSFLKSDIYLE YTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNE DEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETA APRVSSSRRYSEGREFRYGSWREPVNPYYVNAG YALAPATSANDSEQQSLSSDADTLSLTDSSVDGI PPYRIRKQHRREMQESVQVNGRVPLPHIPRTYR VPKEVRVEPQKFAEELIHRLEAVQRTREAEEKL EERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPA WHHFPPRCVDMGCAGLRDAHEENPESILDEHVQ RVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGA ASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHST ARPKEQVEAEATRRAQSSFAWGLEPHSHGARSR GYSESVGAAPNASDGLAHSGKVGVACKRNAKKA ESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEI SRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAG PQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEE ARRRLEEEEKRASRAPSKQRTRSQRKVGGGSAQP CDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKE LLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEA VLPVFEEKIIGKVEKVD**AGGLAAVPGEALGGH GCHGQADDLVLRSPMGNSVGCTTHPCGLPVSRG R*GVPPSGPQASVLPPRRQPGPLLIAILGGPSP SEAAEVPAASFR*PPPIPRHLPWARVLGSTFPLG FAHVPRSGWGPGPDAEACRASVNCTRHRVPSTQ LVSCLPLCESATEHCTCLQPPAVQSWVLLSEGP CKYVHFSG*ASRGCPSLFFMR*HLYN*LSFQRYL DNNEIKLFLNL

Frame 1

SGGDRGRCRRQSRPESARSRVHAERAGGSWRLG PCGAPAACPASASGPPSRSRRDGGRPGPRPRRV PPAALPLSAWARTAPRRSGSRARGPATTARGTD* FTLEL*VLMY*GAALIVP*RRVPK*ISKSRVSP WTSEQVSPKMLPDPQCLVRRENWCPQTRGPPAT VSAPGKVLALKVRLRRPLRGARIWTWGMSLRAVP PPPHHT*SGLSHCIPCWMTKMG*ACSGLS*SRR AVPTCWTSGLPALASGSWSPVTRTRRRG*SWRE PSTESTFLITMASCPGRPSQPPRAS*RAAS*SS* SILPCLTRPRPKSRPLWRKTPIPPSLSLIFIWN IRGQARRAPKSVVTRALGQGQGRAYLDTCRP*M KMRNGSVTRTWMRTMAETLLPPEDSLRSCSWRQL PRGSPPVDGTAKAESSGMDPGGSQSTPIMSMPA MPWPQPPVPTTASSRACPAMQTPCPSRTAAWMG SPHTGSVSSTAGRCRRACRSMGGCPYLTFPARTG CRRRSAWSLRSSRRSSSTAWRLCSARGRPRRSW RSG*SACAWRRKVRTAIHRQGPQGRVTSCLPPP LGTTSRPAVWTWAVPGSGMHTRRTLRASWTSTYS VC*GHLAASRLGLAIAPRTVGTWPRCQWHWGVP PRGTGSTYPSQGRSWTRPACTTTDTSTTTSTTA QPGPRSRWRPRPPAGPRAASPGAWNHTAMGQGPE ATQRVLALPPTPVMASPTVGRWAWRAKEMPRRL SRGRAPAPRCQVPRRMRRRTRKSCSGSLRGKRR SAGTAGPATGLRGRGSHSPMRTPDPCPLSTPGPA LSSGPPCSPPTSSSKTPPCHPTQLPTP*PSWRR RADVWRRKKREPAEHPPSRGQDRRGRWAAGVPS RVTASLWRTTSAGNPSPTAPW*GAALSPWASSRS C*PKRAATDTTSRK*ATSLTVGWCLRRFERTRP SCPSLRRRSSAKWRRWTDRLVGWPLCQARPLAG TGVTARQMTSYSGARWGTVLGVPPIPVVYPCLEA GRGSLQVVHKLLSCPQGGSLDHSS*QYLEGPAQ VRQPRSLLPASGDPPPSPGTSLGHVCWDLLSLW DLPTYPGLGGAQARMQRPAGPLSIVRATECLQHS LSLACHCVNRRRSTAPASSRRLCSPGSSFLRAR VNMYISQARPAGAARVCFSCDDTCTINYLFKGT WIIMK*NCF*TC

Frame 2

REAIVAAAAARAGRSRPGAASTRSGRVGAGGWA PAAPPRPAPRPPPGRRAAAAEMGAAPGRAPAGS RPPRCR*AHGPGPRRAAPGAGPGVPPPPRAGQI DSLWSCKY*CIRVQRSLFLDAESQNEYPRAGFPL GPRSKFHRRCSPTPSAW*GGRTGVHRPEARQLQ FLLRERCWH*R*DFDGHSEALGSGPGV*A*GQC LPHPTILEVG*VTAFPAG*PRWDKPVQDFPEAGG LCRLAGLLVCLHWLQEAGAL*LERGEEAEAGES HLPKVHS**QWHRVPADQASHQELHKGLHHEAA DRSCHV*PGPDRNPGHYGGKHLSLLP*V*YLFGI YEDRLGEPQSL**PELWVRDREGHIWIPADLK* R*GMEV*PGHG*GRWQRRCSPRKTPSEAAPGDS CPEGLLQ*TVQRRQRVQVWILAGASQPLLCQCRL CPGPSHQCQRQRAAEPVQRCRHPVPHGQQRGWD PPIQDP*AAPQGDAGERAGQWAGAPTSHSPHVP GAEGGPRGASEVRGGAHPPPGGCAAHAGGRGEAG GAAEARAHGGGR*GRRSIVRAPRAVSQAASRPR LAPLPAPLCGHGLCRAPGCTRGEP*EHPGRART ACAEDTWPPVAWAWPSLPGQWARGQDASGTGGCR LGAREARTQVRGEAGRGRPAPPPTRPPPRPPQH SPAQGAGGGRGHPQGPEQLRLGPGTTQPWGKVP RLLRECWRCPQRQ*WPRPQWEGGRGVQKKCQEG* VGEERQHRGARCLGGCGEEPENHAVDH*GGKGD QQAPQDRPRVFGDEEATAP*ELQTLVP*APLGR PSAPDLRAALPPLHPRPHHATPPSSQPPNPAGGG APTSGGGRKESQPSTLQAEDKIAEEGGRRECPA V*QHRCGVLLLRGTHPLPHPGEGPRCHPGPVQG AADQKGQLQILLQESERRV*LWGGV*GGSRGRGR PARL*GEDHRQSGEGGLIGWWAGRCARRGPWRA RVSRPGR*PRTQEPDGEQCWVYHPSLWSTRV*R QVGGPSKWSTSFCPAPKEAAWTTPHSNTWRAQPK *GSRGPCCQLQVTPPHPPAPPLGTCAGIYFPSG ICPRTQVWVGPRPGCRGLQGLCQLYAPPSAFNT ACLLPATV*IGDGALHLPPAAGCAVLGPPF*GPV *ICTFLRLGQQGLPESVFHAMTLVQLIIFSKVL G***NKTVFEP

Sequence AXIN1.cAug05

CTTTGGAGCTGTAAGTACTGATGTATTAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTTCCCCTTGGAC CTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCT GCTCCGGGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCC CACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGT GCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCT ACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGA TCCTGCCATGTTTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAATATACG AGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGAAGATG AGGAATGGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCC GAGGGTCTCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCAATGCCGGCTATGCC CTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCC CATACAGGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCACGTACCGGGTGCC GAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAG CGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGC ACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCCTGAGAGCATCCTGGACGAGCACGTACAGCGTGT GCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCG GGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCACAGCCC GGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTA CTCAGAGAGTGTTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTGAGTCG GGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCA GGCACCGCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCA GCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGGCGCGC CGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGTACGGCCGCGTCGTGGGGTGGGGTGGGTCTTGGGGGTTACCACAGC ACCACTCCTGGGGCTGAAGCTCCCCGAGGCCTGAGGTCTTCAGGGGAAAAACGGCCCTCTGGGTGTCCCCCAGCCATACTGGGCCCTTCCATCCACTGAG AACCACTGAGGGGCCACGTGGCAGGCCACCCTGCGGTGGACGGAGGCTGGGGCTGGAGCTTTCACACACCCGAGGCCGCCGCGCTCATTGCTCTGGAGGC GGGGTCCCTCCTCAACCTGAGCCCAGTCTGCAGGGAGGCTGCAGGGCCCCAGTGTGTGGGGAACGCCGGTCCCCAGCCAGAGGCTGCTCGCTGCTGGGCA GCCTGGGCTTTAGGGCAGAGGTCCTGGAGGCATGGCGGCTGCCTCTTGACAGTTGCGTCCCAGGAGCCCTGAGTGGCTCTGCACGGTCGGAAAGCTACGT GGTTCTGAAAGCCGTGCAGATGTAGAAGTGTCGTCCTGGACGTCCAGCCCACAGCCCTCCTCGCTGTGTCCTCTTGTCATCCTGGATGTCCAGCCCACAG CCCTCCTCACTGTGCCCTTCTCTCATCCGGGACGTCCAGCCCACAGCCCTCCTCACTGCGCCCTCCTTGGTCTTCTGGGCTCCGTAGCCAGACCCTGTTC TCTCCCTAGGGCAGGGAGGCTTCTGTCTGGGCTGCAGGGAGGTCTCCCCTTTGTGTGGGCAGCATTTTCGCTCCAGGCCAGAACCTGGCTTCTCCATTTT CAGCTTGCTCATAAGGCCAGTTTTCTCGGCCATCCTGGGTCATTGGAATTCCCCACAGATCAGTGTCTCTTTGAGGGTCCTGGGAGCCTATAGCTCACCC AGGCCCTGGGGCTGTGGTCACAGAGGCCATGGTTGCCTTCAGAGGTCACCAGCTCCTCCTGTCCACCTTGGGTCACCTGGGGCCTGGGGTCCAGGCCCGC CTCCCGGGCCTCCAGCTTTGGGGCTGTGCCACTCCAGGGAGGCTGTGGCTAGGGGCCTTATTCTGCCGAACCAGCATCAGGGTTCAGACCCTTCCCAGCT GAAAGCCTGTGTGGTCTCAAGTCATTAAAGGTTCCTGAGCTTCTGCCAGGCTGTGAGCCTAATGACAGCTCTGGGGCCAGAAAGGGTCACAGTTACCTCA AACCCAAAGGGTGACCACTTTATGACCAAACATCTGTGACCCTGTGATCGTGTTCGGCCAACAAGGAGCTGGAGCCCCCTGCCATGCAGGAGCCTGGAGT GTGGTGCTCGGAGCCGGGGCGTGTGACTGGGCTGGCAGATGAGCCGTGGTGGGCATCGTGTGCAGCAGGTCCGAGGCCCTGGGCGTGTGTGGGCTCTCCT GCCGGGGCTTCCTGAGCCATCCTTGCTGGGCTGAGCAGGGGTCTCTCAGGTTGCGTCTGCGCTTTCCCCTGGTACCCGTGGCCCCACATGATGGCAGGGG ATGCCCGCTGAGCCGTGGGACCACTCCCGCCGTTCTGAGACACGACCCTCCCGCCCGTTTGGCTGCTGGGCTCCAGCATCCCCCCATGGGGAAACCCAGA GTTGCTGGGTTTGTCCGTCCAGGGGATGTGGGTTTTTGCGAGAAGCTGTGGAGACGGAGTGAAGGCCCAGAGGCAGGTTGGGTGCTGGGCCCTGTCAGGA TGTCAGAGTAGAAACCACCATCAAGGTCGCCCAGTGTACCCCGGTGGAGGTGTCCACGTGAGAGGAGCTGCTGTGCTGCCCCAGGATGGCCTCAGTTGTG GCGCCGGGGGGCCCTCAGCCTCCCTGAGGTGCAGACCGAGTTGCCACGCACGCCCCAGAGCCAGTGTCCACGCTATCGGTGGGGAATTCATGCCAAGAGA CTGAAGGACAGACTCTCGGGACGAGATGTCATCCCACCAGAGGGGATGAGGTGGTGGGATCCCAGCACCCCTGATCGGAAGCTCCCCAAAGTGCGGTCCA GGGAACTCAGGGGGTTGGTGCCGGGATCATGAATGTGGGGCGCGCTGCAGTATCGGGTACCACCAAGGCAGGCCTAGGAGGGGACTCGGTGTCTTGTCTA CCCTGCAAACTGGAGCCTGCGCAGGGTGGGTTGTTCGGGCCCTTCACGCTCCGTCCGTCCCTGCGCAGCAGCATTGGTCGAGGCTCCCTCCCCAGGGCTG GTGGCCGCCGGCACAGCCACCTTTGACGCGGGTTGTTCCCTAGGTATGTGCAGGAGGTTATGCGGCGGGGACGCGCCTGCGTCAGGCCAGCGTGCGCGCC GGTGCTGCACGTGGTACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGTACGTGCGTCCTGAGGGTCCTCCCGGTCCTGCCCACCCCAGCCCTGGC GTCCCAAGCCAGGTCCCATCGTAGAGCCGCCGTCCCGGGGCTTGAGCTCGGGTACCACGCTCAGAAGCAGCTGTGGCAGGAGAGGGTCCCGTGGCATCAG ACTCGAAGCTGCCCGAGAGCCTTCTCCTGTCTCCATCAAAGGAGACGCTGACTGTCCAGCATGATGACAAGTGACCAAAAGGCCCGGCTGGAGGCCTGGG CACAGCCAGCCCCTGCATCCACACAGGTGTCACGGCCAGGCTTCCTGTGGGGCAGAGACCTGCCTGCGCCTCCACCCGGCTTGGAACGGGCCGGTCCCTG CCAGGCCTGACTTGACCAGGCTCGGGGCTGCAGGTGCTGAGGTGCCCGGGGTGCGTCTGCTTGCAGTGTGGGTCTCAGCCATGCGTGTCTGTCCAGCCCG TGCAGTCAGTGAGTCGGGGCTCTGCTCCCACCTACAATGCTGACTGCTCTGAACCCGTGAGAAAGGGCTCAGACCATGTGATCCCACATGGGCCGTGTCG ACGGCGCCTTTATGTGCTCAAGGTGGCAGGAGGAGGGGGCACACCCCAAAAGGGGCCTTGAGAACAAGGGTCCCCCAGGCCTTGCCAGGCTTGCCCCAGA TGCAGGTTTGTTGTGGCCCTCATCGTGTAGGCGGTCTTGGGGCAGCCTTCACCTGTCCCTGGGGCAGCCCCCACGTGTTGGCTCTTCTCTGATGGGCCTG GCTCCAGGAGGCTCCGAGGATGTGGCACTGGGAGGACGTCTAAGAGCGCACGTGGCTGGGGGCCTGGTGTGCAAAGAGGATGGTCACATGGCTCAGGTGG CCATGAGGCCTTCCTGCCCTGGTGTTGGCTCTGGAAGCCTCTGGTGCACCCAGCCAGGAGGAGGCCCAAGGGCTGAACCCCAGTGGGCCAGGGGCAGCTT CTGGAGTCACTTGAAGGACAGGTGGCGGCGGGGTAGCCGAGTGGGGGGTGCGAGAGCAACCAGGCCCCGCCCGTCATCCCGGCACGGCTGTGGAAGCACA GCACCTGTCAGAGGAGGGCCCGGCTCAGCCTGGGCAGGAGACGGTGCCAGCCCTGCCCCCTCTGAAGGGCCTGCTGGCCACCCCCGCCCCAGTCCTCCGA CAAACTTCGCTGCGGGGTCAGGCTTATCCAGAAATCGTGCACAGTTCTGTTTGTCCAATCCCTGCCCTTCAGATGTTTTTAAGGTTTGGGGGCCGTAGGT CTTCCTGAGATAGGAGCCCTGCAGCCTGCCCTGCCATCCTAGAGGTGGGAGCTGCAGTGCACCTGGCAGCCAGGCCCTGGCCAGTCGAGGGTGGGGCTGT GAGGCAGCAGGGAGACCTTCGTGCTCCCAGTGAGCGGAGCCGACTCCACTGCTAGCGGACTAGATCCCAGATTGAGATGCTGTGCGGGGGCTGCGGGCCC CAGGTGTTTGAATGGGGTGCTGCTCACCCCTGCAGGCCTGCCCCGCCTTACACTGGCAGCCTGAGGCCAGCAGGAGGGACCCTGTGGGACCTCAGTCCAA AACCAGGTACCACCGCTGCATCTCTTTCCCCAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTTGTGGCGTAC TACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCTACA GATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGAT CATCGGCAAAGTGGAGAAGGTGGACTGATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTTGGCGGGCACGGGTGTCACGGCCAGGCAGATGAC CTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCCATCCCTGTGGTCTACCCGTGTCTAGAGGCAGGTAGGGGGTCCCTCCAAGTGGTC CACAAGCTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGCCCAGCCCAAGTGAGGCAGCCGAGGTCCCTGCTGC CAGCTTCAGGTGACCCCCCCCCATCCCCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTCCCTCTGGGATTTGCCCACGTACCCAGGTCTGGG TGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATTGTACGCGCCACCGAGTGCCTTCAACACAGCTTGTCTCTTGCCTGCCACTGTGTG AATCGGCGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTGAGGGCCCGTGTAAATATGTACATTTCTCAGGCTA GGCCAGCAGGGGCTGCCCGAGTCTGTTTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTTGGATAATAATGAAATAAAACTGTTT TTGAACCTGC

AXIN1-3 (forward primer)
AXIN1-4 (reverse primer)
Intron retention

Frame 0

LWSCKY*CIRVQRSLFLDAESQNEYPRAGFPLG PRSKFHRRCSPTPSAW*GGRTGVHRPEARQLQFL LRERCWH*R*DFDGHSEALGSGPGV*A*GQCLP HPTILEVG*VTAFPAG*PRWDKPVQDFPEAGGL CRLAGLLVCLHWLQEAGAL*LERGEEAEAGESHL PKVHS**QWHRVPADQASHQELHKGLHHEAADR SCHV*PGPDRNPGHYGGKHLSLLP*V*YLFGIY EDRLGEPQSL**PELWVRDREGHIWIPADLK*R* GMEV*PGHG*GRWQRRCSPRKTPSEAAPGDSCP EGLLQ*TVQRRQRVQVWILAGASQPLLCQCRLC PGPSHQCQRQRAAEPVQRCRHPVPHGQQRGWDPP IQDP*AAPQGDAGERAGQWAGAPTSHSPHVPGA EGGPRGASEVRGGAHPPPGGCAAHAGGRGEAGG AAEARAHGGGR*GRRSIVRAPRAVSQAASRPRLA PLPAPLCGHGLCRAPGCTRGEP*EHPGRARTAC AEDTWPPVAWAWPSLPGQWARGQDASGTGGCRL GAREARTQVRGEAGRGRPAPPPTRPPPRPPQHSP AQGAGGGRGHPQGPEQLRLGPGTTQPWGKVPRL LRECWRCPQRQ*WPRPQWEGGRGVQKKCQEG*V GEERQHRGARCLGGCGEEPENHAVDH*GGKGDQQ APQDRPRVFGDEEATAP*ELQTLVP*APLGRPS APDLRAALPPLHPRPHHATPPSSQPPNPAGGGA PTSGGGRKESQPSTLQAEVRPRRGVGWVLGVTTA PLLGLKLPEA*GLQGKNGPLGVPQPYWALPSTE NH*GATWQATLRWTEAGAGAFTHPRPPRSLLWR RGPSST*AQSAGRLQGPSVWGTPVPSQRLLAAGQ PGL*GRGPGGMAAAS*QLRPRSPEWLCTVGKLR GSESRADVEVSSWTSSPQPSSLCPLVILDVQPT ALLTVPFSHPGRPAHSPPHCALLGLLGSVARPCS LPRAGRLLSGLQGGLPFVWAAFSLQARTWLLHF QLAHKASFLGHPGSLEFPTDQCLFEGPGSL*LT QALGLWSQRPWLPSEVTSSSCPPWVTWGLGSRPA SRASSFGAVPLQGGCG*GPYSAEPASGFRPFPA ESLCGLKSLKVPELLPGCEPNDSSGARKGHSYL KPKG*PLYDQTSVTL*SCSANKELEPPAMQEPGV WCSEPGRVTGLADEPWWASCAAGPRPWACVGSP AGAS*AILAGLSRGLSGCVCAFPWYPWPHMMAG DAR*AVGPLPPF*DTTLPPVWLLGSSIPPWGNPE LLGLSVQGMWVFARSCGDGVKAQRQVGCWALSG CQSRNHHQGRPVYPGGGVHVRGAAVLPQDGLSC GAGGPSASLRCRPSCHARPRASVHAIGGEFMPRD *RTDSRDEMSSHQRG*GGGIPAPLIGSSPKCGP GNSGGWCRDHECGARCSIGYHQGRPRRGLGVLS TLQTGACAGWVVRALHAPSVPAQQHWSRLPPQGW WPPAQPPLTRVVP*VCAGGYAAGTRLRQASVRA GAARGTSRVGHGALRDRVRAS*GSSRSCPPQPW RPKPGPIVEPPSRGLSSGTTLRSSCGRRGSRGIR LEAAREPSPVSIKGDADCPA**QVTKRPGWRPG HSQPLHPHRCHGQASCGAETCLRLHPAWNGPVP ARPDLTRLGAAGAEVPGVRLLAVWVSAMRVCPAR AVSESGLCSHLQC*LL*TREKGLRPCDPTWAVS TAPLCAQGGRRRGHTPKGALRTRVPQALPGLPQ MQVCCGPHRVGGLGAAFTCPWGSPHVLALL*WAW LQEAPRMWHWEDV*ERTWLGAWCAKRMVTWLRW P*GLPALVLALEASGAPSQEEAQGLNPSGPGAA SGVT*RTGGGGVAEWGVREQPGPARHPGTAVEAQ HLSEEGPAQPGQETVPALPPLKGLLATPAPVLR QTSLRGQAYPEIVHSSVCPIPALQMFLRFGGRR SS*DRSPAACPAILEVGAAVHLAARPWPVEGGAV RQQGDLRAPSERSRLHC*RTRSQIEMLCGGCGP QVFEWGAAHPCRPAPPYTGSLRPAGGTLWDLSP KPGTTAASLSPGQDRRGRWAAGVPSRVTASLWRT TSAGNPSPTAPW*GAALSPWASSRSC*PKRAAT DTTSRK*ATSLTVGWCLRRFERTRPSCPSLRRR SSAKWRRWTDRLVGWPLCQARPLAGTGVTARQMT SYSGARWGTVLGVPPIPVVYPCLEAGRGSLQVV HKLLSCPQGGSLDHSS*QYLEGPAQVRQPRSLL PASGDPPPSPGTSLGHVCWDLLSLWDLPTYPGLG GAQARMQRPAGPLSIVRATECLQHSLSLACHCV NRRRSTAPASSRRLCSPGSSFLRARVNMYISQA RPAGAARVCFSCDDTCTINYLFKGTWIIMK*NCF *TC

Frame 1

FGAVSTDVLGCSAHCSLTQSPKMNIQEQGFPLD LGASFTEDAPRPPVPGEEGELVSTDPRPASYSF CSGKGVGIKGETSTATPRRSDLDLGYEPEGSASP TPPYLKWAESLHSLLDDQDGISLFRTFLKQEGC ADLLDFWFACTGFRKLEPCDSNEEKRLKLARAI YRKYILDNNGIVSRQTKPATKSFIKGCIMKQLID PAMFDQAQTEIQATMEENTYPSFLKSDIYLEYT RTGSESPKVCSDQSSGSGTGKGISGYLPTLNED EEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAP RVSSSRRYSEGREFRYGSWREPVNPYYVNAGYA LAPATSANDSEQQSLSSDADTLSLTDSSVDGIP PYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVP KEVRVEPQKFAEELIHRLEAVQRTREAEEKLEE RLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAW HHFPPRCVDMGCAGLRDAHEENPESILDEHVQRV LRTPGRQSPGPGHRSPDSGHVAKMPVALGGAAS GHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTA RPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGY SESVGAAPNASDGLAHSGKVGVACKRNAKKAES GKSASTEVPGASEDAEKNQKIMQWIIEGEKEIS RHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQ LRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEAR RRLEEEEKRASRAPSKQRYGRVVGWGGSWGLPQ HHSWG*SSPRPEVFRGKTALWVSPSHTGPFHPLR TTEGPRGRPPCGGRRLGLELSHTRGRRAHCSGG GVPPQPEPSLQGGCRAPVCGERRSPARGCSLLG SLGFRAEVLEAWRLPLDSCVPGALSGSARSESYV VLKAVQM*KCRPGRPAHSPPRCVLLSSWMSSPQ PSSLCPSLIRDVQPTALLTAPSLVFWAP*PDPV LSLGQGGFCLGCREVSPLCGQHFRSRPEPGFSIF SLLIRPVFSAILGHWNSPQISVSLRVLGAYSSP RPWGCGHRGHGCLQRSPAPPVHLGSPGAWGPGP PPGPPALGLCHSREAVARGLILPNQHQGSDPSQL KACVVSSH*RFLSFCQAVSLMTALGPERVTVTS NPKGDHFMTKHL*PCDRVRPTRSWSPLPCRSLE CGARSRGV*LGWQMSRGGHRVQQVRGPGRVWALL PGLPEPSLLG*AGVSQVASALSPGTRGPT*WQG MPAEPWDHSRRSETRPSRPFGCWAPASPHGETQ SCWVCPSRGCGFLREAVETE*RPRGRLGAGPCQD VRVETTIKVAQCTPVEVST*EELLCCPRMASVV APGGPQPP*GADRVATHAPEPVSTLSVGNSCQE TEGQTLGTRCHPTRGDEVVGSQHP*SEAPQSAVQ GTQGVGAGIMNVGRAAVSGTTKAGLGGDSVSCL PCKLEPAQGGLFGPFTLRPSLRSSIGRGSLPRA GGRRHSHL*RGLFPRYVQEVMRRGRACVRPACAP VLHVVPAVSDMELSETEYVRPEGPPGPAHPSPG VPSQVPS*SRRPGA*ARVPRSEAAVAGEGPVAS DSKLPESLLLSPSKETLTVQHDDK*PKGPAGGLG TASPCIHTGVTARLPVGQRPACASTRLGTGRSL PGLT*PGSGLQVLRCPGCVCLQCGSQPCVSVQP VQSVSRGSAPTYNADCSEPVRKGSDHVIPHGPCR RRLYVLKVAGGGGTPQKGP*EQGSPRPCQACPR CRFVVALIV*AVLGQPSPVPGAAPTCWLFSDGP GSRRLRGCGTGRTSKSARGWGPGVQRGWSHGSGG HEAFLPWCWLWKPLVHPARRRPKG*TPVGQGQL LESLEGQVAAG*PSGGCESNQAPPVIPARLWKH STCQRRARLSLGRRRCQPCPL*RACWPPPPQSSD KLRCGVRLIQKSCTVLFVQSLPFRCF*GLGAVG LPEIGALQPALPS*RWELQCTWQPGPGQSRVGL *GSRETFVLPVSGADSTASGLDPRLRCCAGAAGP RCLNGVLLTPAGLPRLTLAA*GQQEGPCGTSVQ NQVPPLHLFPQDKIAEEGGRRECPAV*QHRCGV LLLRGTHPLPHPGEGPRCHPGPVQGAADQKGQLQ ILLQESERRV*LWGGV*GGSRGRGRPARL*GED HRQSGEGGLIGWWAGRCARRGPWRARVSRPGR* PRTQEPDGEQCWVYHPSLWSTRV*RQVGGPSKWS TSFCPAPKEAAWTTPHSNTWRAQPK*GSRGPCC QLQVTPPHPPAPPLGTCAGIYFPSGICPRTQVW VGPRPGCRGLQGLCQLYAPPSAFNTACLLPATV* IGDGALHLPPAAGCAVLGPPF*GPV*ICTFLRL GQQGLPESVFHAMTLVQLIIFSKVLG***NKTV FEP

Frame 2

LEL*VLMY*GAALIVP*RRVPK*ISKSRVSPWT SEQVSPKMLPDPQCLVRRENWCPQTRGPPATVS APGKVLALKVRLRRPLRGARIWTWGMSLRAVPP PPHHT*SGLSHCIPCWMTKMG*ACSGLS*SRRAV PTCWTSGLPALASGSWSPVTRTRRRG*SWREPS TESTFLITMASCPGRPSQPPRAS*RAAS*SS*S ILPCLTRPRPKSRPLWRKTPIPPSLSLIFIWNIR GQARRAPKSVVTRALGQGQGRAYLDTCRP*MKM RNGSVTRTWMRTMAETLLPPEDSLRSCSWRQLP RGSPPVDGTAKAESSGMDPGGSQSTPIMSMPAMP WPQPPVPTTASSRACPAMQTPCPSRTAAWMGSP HTGSVSSTAGRCRRACRSMGGCPYLTFPARTGC RRRSAWSLRSSRRSSSTAWRLCSARGRPRRSWRS G*SACAWRRKVRTAIHRQGPQGRVTSCLPPPLG TTSRPAVWTWAVPGSGMHTRRTLRASWTSTYSV C*GHLAASRLGLAIAPRTVGTWPRCQWHWGVPPR GTGSTYPSQGRSWTRPACTTTDTSTTTSTTAQP GPRSRWRPRPPAGPRAASPGAWNHTAMGQGPEA TQRVLALPPTPVMASPTVGRWAWRAKEMPRRLSR GRAPAPRCQVPRRMRRRTRKSCSGSLRGKRRSA GTAGPATGLRGRGSHSPMRTPDPCPLSTPGPAL SSGPPCSPPTSSSKTPPCHPTQLPTP*PSWRRRA DVWRRKKREPAEHPPSRGTAASWGGVGLGGYHS TTPGAEAPRGLRSSGEKRPSGCPPAILGPSIH* EPLRGHVAGHPAVDGGWGWSFHTPEAAALIALEA GSLLNLSPVCREAAGPQCVGNAGPQPEAARCWA AWALGQRSWRHGGCLLTVASQEP*VALHGRKAT WF*KPCRCRSVVLDVQPTALLAVSSCHPGCPAHS PPHCALLSSGTSSPQPSSLRPPWSSGLRSQTLF SP*GREASVWAAGRSPLCVGSIFAPGQNLASPF SACS*GQFSRPSWVIGIPHRSVSL*GSWEPIAHP GPGAVVTEAMVAFRGHQLLLSTLGHLGPGVQAR LPGLQLWGCATPGRLWLGALFCRTSIRVQTLPS *KPVWSQVIKGS*ASARL*A**QLWGQKGSQLPQ TQRVTTL*PNICDPVIVFGQQGAGAPCHAGAWS VVLGAGACDWAGR*AVVGIVCSRSEALGVCGLS CRGFLSHPCWAEQGSLRLRLRFPLVPVAPHDGRG CPLSRGTTPAVLRHDPPARLAAGLQHPPMGKPR VAGFVRPGDVGFCEKLWRRSEGPEAGWVLGPVR MSE*KPPSRSPSVPRWRCPRERSCCAAPGWPQLW RRGALSLPEVQTELPRTPQSQCPRYRWGIHAKR LKDRLSGRDVIPPEGMRWWDPSTPDRKLPKVRS RELRGLVPGS*MWGALQYRVPPRQA*EGTRCLVY PANWSLRRVGCSGPSRSVRPCAAALVEAPSPGL VAAGTATFDAGCSLGMCRRLCGGDAPASGQRAR RCCTWYQPCRTWSSPRQSTCVLRVLPVLPTPALA SQARSHRRAAVPGLELGYHAQKQLWQERVPWHQ TRSCPRAFSCLHQRRR*LSSMMTSDQKARLEAW AQPAPASTQVSRPGFLWGRDLPAPPPGLERAGPC QA*LDQARGCRC*GARGASACSVGLSHACLSSP CSQ*VGALLPPTMLTALNP*ERAQTM*SHMGRV DGAFMCSRWQEEGAHPKRGLENKGPPGLARLAPD AGLLWPSSCRRSWGSLHLSLGQPPRVGSSLMGL APGGSEDVALGGRLRAHVAGGLVCKEDGHMAQV AMRPSCPGVGSGSLWCTQPGGGPRAEPQWARGSF WSHLKDRWRRGSRVGGARATRPRPSSRHGCGST APVRGGPGSAWAGDGASPAPSEGPAGHPRPSPP TNFAAGSGLSRNRAQFCLSNPCPSDVFKVWGP*V FLR*EPCSLPCHPRGGSCSAPGSQALASRGWGC EAAGRPSCSQ*AEPTPLLAD*IPD*DAVRGLRA PGV*MGCCSPLQACPALHWQPEASRRDPVGPQSK TRYHRCISFPRTRSQRKVGGGSAQPCDSIVVAY YFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSY RYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKI IGKVEKVD**AGGLAAVPGEALGGHGCHGQADD LVLRSPMGNSVGCTTHPCGLPVSRGR*GVPPSG PQASVLPPRRQPGPLLIAILGGPSPSEAAEVPAA SFR*PPPIPRHLPWARVLGSTFPLGFAHVPRSG WGPGPDAEACRASVNCTRHRVPSTQLVSCLPLC ESATEHCTCLQPPAVQSWVLLSEGPCKYVHFSG* ASRGCPSLFFMR*HLYN*LSFQRYLDNNEIKLF LNL