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Sequences

Sequence PCBP4.dAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGCCTCTGGAGTCCCTGTGGCAGAACTCAAATGGAAGGAGGAGCAGCTGCACCCAGGGGAGCAGGGTGTCACC TCCGTTGGATAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGAGCGGCTCGGACGGGGGACTG GAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGC GAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACAGCAGCTGTCTTCCATGC AGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATC CCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGA GCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCA GCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGAT TTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTG GGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGG GACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCC ATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCA AGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGC AGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCT CCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCT GTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTG CATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTT TTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCA GCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAATAAATATTCTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSEPLESLWQNSNGRRSSCTQGSRVSP PLDRAGQVAARTHSQVAVAPASPDRMSGSDGGL EEEPELSITLTLRMLMHGKEVGSIIGKKGETVK RIREQSSARITISEGSCPERITTITGSTAAVFHA VSMIAFKLDEDLCAAPANGGNVSRPPVTLRLVI PASQCGSLIGKAGTKIKEIRESPPKGATIPYHP SLSLGTVLLSANQGFSVQGQYGAVTPAEVTKLQQ LSSHAVPFATPSVVPGLDPGTQTSSQEFLVPND LIGCVIGRQGSKISEIRQMSGAHIKIGNQAEGA GERHVTITGSPVSIALAQYLITACLETAKSTSGG TPSSAPADLPAPFSPPLTALPTAPPGLLGTPYA ISLSNFIGLKPMPFLALPPASPGPPPGLAAYTA KMAAANGSKKAERQKFSPY*GQLRYRQGQAGPPA GGCLCTLPAQGDSTLGSQTPLTPRRMDAPPTLP PSMGVLSLRVGAVSGPGV*AAAAPGQGALPPQL CAWIQGAARRLPSAPTMAGVTHALPIP*GFLAYC ILVGVREEGPLGSWGQASLPTTCRFLAASTDTL LTGMNWLGLSGGTPKRGHCQVAGGVAWGRGPVL SSRHSVQFFQSLFLNKYSQRPG

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPASLWSPCGRTQMEGGAAAPRGAGCH LRWIEPARLQRGHTRRSLWPQPRLTE*AARTGDW RRSQSSASPSRCGC*CTGRKWAASSGRRARL*S ESGSRAVPGSPSPRAPALNASPPSPGLQQLSSM QSP*LLSNWMRTFVLLLQMVEMSPGLQ*PCALSS LPVSVAH*LGRLAPRSRRSESPHPKEPLSPTIR ASP*VLFFSLPTRASLSRVSMGL*PQLRSPSSS SSQAMRSPLPHPAWCQDWIPAHRPAHRSSWFPTI *LAV*SGARAARSARSGRCQGHISRSGTKQRAL GSGMSPSLALRSPSPWPSTSSLPV*RRPSLPLG GRPARPPQTCLPPSRHP*RPCPQLPLACWAHPMP SPSPTSSASSPCPSWLYHLLPQGRRRAWRPTLP RWQQLMGARRLSGRNSPPTEAS*GTGRGRQDHQ QGAASAPYPPKETPPWGPKRR*RPDAWMHPLPCL HLWEFFLSEWGQFLAQGSELRQPQGRGPYLLSS VLGYREQPGDSLVPPPWRVSLTHSPSLRASWPT ASLWESGRRARWVAGARLLSPPPADFLLLPLIPF *LE*TGWACQGAPQRGGTAR*LGEWHGAGAQFS AADTLYSFFNPCF*INILSDQE

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQRASGVPVAELKWKEEQLHPGEQGVT SVG*SRPGCSADTLAGRCGPSLA*QNERLGRGT GGGARAQHHPHAADADAREGSGQHHREEGRDCKA NPGAEQCPDHHLRGLLP*THHHHHRVYSSCLPC SLHDCFQTG*GPLCCSCKWWKCLQASSDPAPCH PCQSVWLTDWEGWHQDQGDPRVPTQRSHYPLPSE PLPRYCSSLCQPGLLCPGSVWGCDPS*GHQAPA ALKPCGPLCHTQRGARTGSRHTDQLTGVLGSQR FDWLCDRAPGQQDQRDPADVRGTYQDREPSRGRW GAACHHHWLSGLHRPGPVPHHCLSRDGQVYLWG DAQLGPRRPACPLLATPDGPAHSSPWPAGHTLC HLPLQLHRPQAHALLGFTTCFPRAAAGLGGLHCQ DGSS*WEQEG*AAEILPLLRPAEVQAGAGRTTS RGLPLHPTRPRRLHPGVPNAANAQTHGCTPYPA SIYGSSFSQSGGSFWPRGLSCGSPRAGGPTSSAL CLDTGSSQETP*CPHHGGCHSRTPHPLGLPGLL HPCGSQGGGPVG*LGPGFSPHHLQISCCFH*YP FDWNELAGLVRGHPKEGALPGSWGSGMGQGPSSQ QQTLCTVFSIPVFE*IFSATR

Sequence PCBP4.iAug05

GGCCCAGCCGCCGTGCCGCCGCCGCCGCCGCCGCAGGACAGCCCCAGCGAGGTAGGGCGCGGGCCGGGCGGGGCACGGCGGGCCGCGAAGTTTGGGGGTT CGCCCGGGGGCAGGGAGCCCGCGCCCAGATCCCTCGGTTGGGGTCGCAGGTTTGTGAGACTGCGAGGAACACGTTCTCTGTCCCACACCGCTGAGGCCTG CACAGGCCATTTCCAGCACATAGAAGAGAGATTGGAAACCAACGTGCAGAACTGCCAGTCCCCTGACACGCTGTGCCCCACCCACTGCAGCCCAGTGCTG AATGAACCCTGCCCAGAGGTGTCTGTAGTGAGCTTCTGCCCTAGTGACTTTTGGTAGGTGGGAGTGTGCCTCAATTCCCCCCTCAACCCCTGCCTCAAGC CTTTACCAGGCAGTGGCAAGACCTGACCACACCCGAGGCCTCCCTGCCTTCAAGGCTTCCCATGGCTGCTCCAGCTTCCTCCCCAGCTGCTCTTCTGTGC TCCATCCACCATCTGGCTGCTGGACGAAAGTGCCTCTCATATGGAAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCG CCTGACAGAATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCA GCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCAC CATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTC TCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGTCCC CACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGC TGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAG ACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCAC ATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGC CTGGTGAGCGCGGGCTGGGCGGCAGTGGGGGAGCAGGTCACGGTTCTCATGTGCCCAAGAAAGGCAGGGGTGGGGAGAGGAAGCTGGCCTCCTCTCTCTG TCTGGGCCCGACCTCTGCCTCTCCTAACCCTACTCCAATTCCCCATGGTCTTTGCCTAATTCACCCTCTGTTGCCCCATCTCCCCCCTCTATATCCACCT CTCATTCTCCATTGCTGTGTCTTTTCCCTGGGTCTCTGGCCACCCCATTTCTCCCTGCACCTCGTGCTATATCTGCTTGTCCTTTCTTCCCTTCCTCTTC CACCTTTCCCATCTTCCCCTTATTGTTCTCTGTTCACTACCTCTCTCTTGCCTTTCATCTAATCTCATGCCCATCTCTGCCCTCATTGCCCCTCTCTCAC TCCCACTTTCCCCTTTGTCTCCCCTCTATATCCCTCTCTCCAGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCT GCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTC AAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGA AGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGC CCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGA GTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGA GACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGG CCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTG TCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTC AATCCCTGTTTTTGAATA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

GPAAVPPPPPPQDSPSEVGRGPGGARRAAKFGG SPGGREPAPRSLGWGRRFVRLRGTRSLSHTAEAC TGHFQHIEERLETNVQNCQSPDTLCPTHCSPVL NEPCPEVSVVSFCPSDFW*VGVCLNSPLNPCLK PLPGSGKT*PHPRPPCLQGFPWLLQLPPQLLFCA PSTIWLLDESASHMEAGQVAARTHSQVAVAPAS PDRMSGSDGGLEEEPELSITLTLRMLMHGKEVG SIIGKKGETVKRIREQSSARITISEGSCPERITT ITGSTAAVFHAVSMIAFKLDEDLCAAPANGGNV SRPPVTLRLVIPASQCGSLIGKAGTKIKEIRES PPKGATIPYHPSLSLGTVLLSANQGFSVQGQYGA VTPAEVTKLQQLSSHAVPFATPSVVPGLDPGTQ TSSQEFLVPNDLIGCVIGRQGSKISEIRQMSGA HIKIGNQAEGAGERHVTITGSPVSIALAQYLITA W*ARAGRQWGSRSRFSCAQERQGWGEEAGLLSL SGPDLCLS*PYSNSPWSLPNSPSVAPSPPSIST SHSPLLCLFPGSLATPFLPAPRAISACPFFPSSS TFPIFPLLFSVHYLSLAFHLISCPSLPSLPLSH SHFPLCLPSISLSPV*RRPSLPLGGRPARPPQT CLPPSRHP*RPCPQLPLACWAHPMPSPSPTSSAS SPCPSWLYHLLPQGRRRAWRPTLPRWQQLMGAR RLSGRNSPPTEAS*GTGRGRQDHQQGAASAPYP PKETPPWGPKRR*RPDAWMHPLPCLHLWEFFLSE WGQFLAQGSELRQPQGRGPYLLSSVLGYREQPG DSLVPPPWRVSLTHSPSLRASWPTASLWESGRR ARWVAGARLLSPPPADFLLLPLIPF*LE*TGWAC QGAPQRGGTAR*LGEWHGAGAQFSAADTLYSFF NPCF*I

Frame 1

AQPPCRRRRRRRTAPAR*GAGRAGHGGPRSLGV RPGAGSPRPDPSVGVAGL*DCEEHVLCPTPLRP AQAISST*KRDWKPTCRTASPLTRCAPPTAAQC* MNPAQRCL**ASALVTFGRWECASIPPSTPASS LYQAVARPDHTRGLPAFKASHGCSSFLPSCSSV LHPPSGCWTKVPLIWKPARLQRGHTRRSLWPQPR LTE*AARTGDWRRSQSSASPSRCGC*CTGRKWA ASSGRRARL*SESGSRAVPGSPSPRAPALNASP PSPGLQQLSSMQSP*LLSNWMRTFVLLLQMVEMS PGLQ*PCALSSLPVSVAH*LGRLAPRSRRSESP HPKEPLSPTIRASP*VLFFSLPTRASLSRVSMG L*PQLRSPSSSSSQAMRSPLPHPAWCQDWIPAHR PAHRSSWFPTI*LAV*SGARAARSARSGRCQGH ISRSGTKQRALGSGMSPSLALRSPSPWPSTSSL PGERGLGGSGGAGHGSHVPKKGRGGERKLASSLC LGPTSASPNPTPIPHGLCLIHPLLPHLPPLYPP LILHCCVFSLGLWPPHFSLHLVLYLLVLSSLPL PPFPSSPYCSLFTTSLLPFI*SHAHLCPHCPSLT PTFPFVSPLYPSLQSRDGQVYLWGDAQLGPRRP ACPLLATPDGPAHSSPWPAGHTLCHLPLQLHRP QAHALLGFTTCFPRAAAGLGGLHCQDGSS*WEQE G*AAEILPLLRPAEVQAGAGRTTSRGLPLHPTR PRRLHPGVPNAANAQTHGCTPYPASIYGSSFSQ SGGSFWPRGLSCGSPRAGGPTSSALCLDTGSSQE TP*CPHHGGCHSRTPHPLGLPGLLHPCGSQGGG PVG*LGPGFSPHHLQISCCFH*YPFDWNELAGL VRGHPKEGALPGSWGSGMGQGPSSQQQTLCTVFS IPVFE

Frame 2

PSRRAAAAAAAGQPQRGRARAGRGTAGREVWGF ARGQGARAQIPRLGSQVCETARNTFSVPHR*GL HRPFPAHRREIGNQRAELPVP*HAVPHPLQPSA E*TLPRGVCSELLP**LLVGGSVPQFPPQPLPQA FTRQWQDLTTPEASLPSRLPMAAPASSPAALLC SIHHLAAGRKCLSYGSRPGCSADTLAGRCGPSL A*QNERLGRGTGGGARAQHHPHAADADAREGSGQ HHREEGRDCKANPGAEQCPDHHLRGLLP*THHH HHRVYSSCLPCSLHDCFQTG*GPLCCSCKWWKC LQASSDPAPCHPCQSVWLTDWEGWHQDQGDPRVP TQRSHYPLPSEPLPRYCSSLCQPGLLCPGSVWG CDPS*GHQAPAALKPCGPLCHTQRGARTGSRHT DQLTGVLGSQRFDWLCDRAPGQQDQRDPADVRGT YQDREPSRGRWGAACHHHWLSGLHRPGPVPHHC LVSAGWAAVGEQVTVLMCPRKAGVGRGSWPPLS VWARPLPLLTLLQFPMVFA*FTLCCPISPLYIHL SFSIAVSFPWVSGHPISPCTSCYICLSFLPFLF HLSHLPLIVLCSLPLSCLSSNLMPISALIAPLS LPLSPLSPLYIPLSSLETAKSTSGGTPSSAPADL PAPFSPPLTALPTAPPGLLGTPYAISLSNFIGL KPMPFLALPPASPGPPPGLAAYTAKMAAANGSK KAERQKFSPY*GQLRYRQGQAGPPAGGCLCTLPA QGDSTLGSQTPLTPRRMDAPPTLPPSMGVLSLR VGAVSGPGV*AAAAPGQGALPPQLCAWIQGAAR RLPSAPTMAGVTHALPIP*GFLAYCILVGVREEG PLGSWGQASLPTTCRFLAASTDTLLTGMNWLGL SGGTPKRGHCQVAGGVAWGRGPVLSSRHSVQFF QSLFLN

Sequence PCBP4.kAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGAGCG GCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAA GGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACA GCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGA CCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGTCCCCACCCAAAGGAGCCAC TATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAG GTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGT TCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAA CCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGGTGAGCGCGGGCT GGGCGGCAGTGGGGGAGCAGGTCACGGTTCTCATGTGCCCAAGAAAGGCAGGGGTGGGGAGAGGAAGCTGGCCTCCTCTCTCTGTCTGGGCCCGACCTCT GCCTCTCCTAACCCTACTCCAATTCCCCATGGTCTTTGCCTAATTCACCCTCTGTTGCCCCATCTCCCCCCTCTATATCCACCTCTCATTCTCCATTGCT GTGTCTTTTCCCTGGGTCTCTGGCCACCCCATTTCTCCCTGCACCTCGTGCTATATCTGCTTGTCCTTTCTTCCCTTCCTCTTCCACCTTTCCCATCTTC CCCTTATTGTTCTCTGTTCACTACCTCTCTCTTGCCTTTCATCTAATCTCATGCCCATCTCTGCCCTCATTGCCCCTCTCTCACTCCCACTTTCCCCTTT GTCTCCCCTCTATATCCCTCTCTCCAGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCCCTTCTCG CCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCT TGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAA ATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACC CTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTG GCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCC CACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGG GGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAA AGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAA TA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSESRPGCSADTLAGRCGPSLA*QNER LGRGTGGGARAQHHPHAADADAREGSGQHHREE GRDCKANPGAEQCPDHHLRGLLP*THHHHHRVY SSCLPCSLHDCFQTG*GPLCCSCKWWKCLQASSD PAPCHPCQSVWLTDWEGWHQDQGDPRVPTQRSH YPLPSEPLPRYCSSLCQPGLLCPGSVWGCDPS* GHQAPAALKPCGPLCHTQRGARTGSRHTDQLTGV LGSQRFDWLCDRAPGQQDQRDPADVRGTYQDRE PSRGRWGAACHHHWLSGLHRPGPVPHHCLVSAG WAAVGEQVTVLMCPRKAGVGRGSWPPLSVWARPL PLLTLLQFPMVFA*FTLCCPISPLYIHLSFSIA VSFPWVSGHPISPCTSCYICLSFLPFLFHLSHL PLIVLCSLPLSCLSSNLMPISALIAPLSLPLSPL SPLYIPLSSLETAKSTSGGTPSSAPADLPAPFS PPLTALPTAPPGLLGTPYAISLSNFIGLKPMPF LALPPASPGPPPGLAAYTAKMAAANGSKKAERQK FSPY*GQLRYRQGQAGPPAGGCLCTLPAQGDST LGSQTPLTPRRMDAPPTLPPSMGVLSLRVGAVS GPGV*AAAAPGQGALPPQLCAWIQGAARRLPSAP TMAGVTHALPIP*GFLAYCILVGVREEGPLGSW GQASLPTTCRFLAASTDTLLTGMNWLGLSGGTP KRGHCQVAGGVAWGRGPVLSSRHSVQFFQSLFLN

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPARAGQVAARTHSQVAVAPASPDRMS GSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKK GETVKRIREQSSARITISEGSCPERITTITGST AAVFHAVSMIAFKLDEDLCAAPANGGNVSRPPV TLRLVIPASQCGSLIGKAGTKIKEIRESPPKGAT IPYHPSLSLGTVLLSANQGFSVQGQYGAVTPAE VTKLQQLSSHAVPFATPSVVPGLDPGTQTSSQE FLVPNDLIGCVIGRQGSKISEIRQMSGAHIKIGN QAEGAGERHVTITGSPVSIALAQYLITAW*ARA GRQWGSRSRFSCAQERQGWGEEAGLLSLSGPDL CLS*PYSNSPWSLPNSPSVAPSPPSISTSHSPLL CLFPGSLATPFLPAPRAISACPFFPSSSTFPIF PLLFSVHYLSLAFHLISCPSLPSLPLSHSHFPL CLPSISLSPV*RRPSLPLGGRPARPPQTCLPPSR HP*RPCPQLPLACWAHPMPSPSPTSSASSPCPS WLYHLLPQGRRRAWRPTLPRWQQLMGARRLSGR NSPPTEAS*GTGRGRQDHQQGAASAPYPPKETPP WGPKRR*RPDAWMHPLPCLHLWEFFLSEWGQFL AQGSELRQPQGRGPYLLSSVLGYREQPGDSLVP PPWRVSLTHSPSLRASWPTASLWESGRRARWVAG ARLLSPPPADFLLLPLIPF*LE*TGWACQGAPQ RGGTAR*LGEWHGAGAQFSAADTLYSFFNPCF* I

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQREPARLQRGHTRRSLWPQPRLTE*A ARTGDWRRSQSSASPSRCGC*CTGRKWAASSGR RARL*SESGSRAVPGSPSPRAPALNASPPSPGLQ QLSSMQSP*LLSNWMRTFVLLLQMVEMSPGLQ* PCALSSLPVSVAH*LGRLAPRSRRSESPHPKEP LSPTIRASP*VLFFSLPTRASLSRVSMGL*PQLR SPSSSSSQAMRSPLPHPAWCQDWIPAHRPAHRS SWFPTI*LAV*SGARAARSARSGRCQGHISRSG TKQRALGSGMSPSLALRSPSPWPSTSSLPGERGL GGSGGAGHGSHVPKKGRGGERKLASSLCLGPTS ASPNPTPIPHGLCLIHPLLPHLPPLYPPLILHC CVFSLGLWPPHFSLHLVLYLLVLSSLPLPPFPSS PYCSLFTTSLLPFI*SHAHLCPHCPSLTPTFPF VSPLYPSLQSRDGQVYLWGDAQLGPRRPACPLL ATPDGPAHSSPWPAGHTLCHLPLQLHRPQAHALL GFTTCFPRAAAGLGGLHCQDGSS*WEQEG*AAE ILPLLRPAEVQAGAGRTTSRGLPLHPTRPRRLH PGVPNAANAQTHGCTPYPASIYGSSFSQSGGSFW PRGLSCGSPRAGGPTSSALCLDTGSSQETP*CP HHGGCHSRTPHPLGLPGLLHPCGSQGGGPVG*L GPGFSPHHLQISCCFH*YPFDWNELAGLVRGHPK EGALPGSWGSGMGQGPSSQQQTLCTVFSIPVFE

Sequence PCBP4.aAug05

CTGTGACCTTGGGCAGGTTACTGATCCTCTCCAGGCATCAGGTTCTCCTGTGTAGTGAATTCATCTGCTCAGCTAACCAGTATGTTAGCTGAGTTCCAGC TGAGCTCAGTATGTTCTGAGCACCTTCTCTGGCCTGGCTCTGAACTGAGCCCTGGGGACATAAGGAGAAGGCAGCAGCCTTGGCTCCTGCTCCAAGGGGC TCCTGGGCTTGGCTGTCCAGAGAAGCAGAAACCAAGCACGTCATCACAAGTGCTTTGATGGGGGAAGTGCAGGCAGCTGGGGGAATGTCTAGCAAGGGCA GCCAGCATGAGGCTGGCCAGGATGCTCGGAGAGCACAGTCCCTGCATCCACTGCTCATCTCCAGCCTCTGGAGTCCCTGTGGCAGAACTCAAATGGAAGG AGGAGCAGCTGCACCCAGGGGAGCAGGGTGTCACCTCCGTTGGATAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCT CGCCTGACAGAATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGG CAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACC ACCATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATG TCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGAC TACGGGTGCCCAGGTACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCCTGTGTGTG CGCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACC AGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAG CGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAG ATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCT CCATCGCCCTGGCCCAGTACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCC CTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATG CCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGC GGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGA CTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCA GTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTA GTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGG TAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGC ACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGT TTTTGAATAAATATTCTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

L*PWAGY*SSPGIRFSCVVNSSAQLTSMLAEFQ LSSVCSEHLLWPGSELSPGDIRRRQQPWLLLQGA PGLGCPEKQKPSTSSQVL*WGKCRQLGECLARA ASMRLARMLGEHSPCIHCSSPASGVPVAELKWK EEQLHPGEQGVTSVG*SRPGCSADTLAGRCGPSL A*QNERLGRGTGGGARAQHHPHAADADAREGSG QHHREEGRDCKANPGAEQCPDHHLRGLLP*THH HHHRVYSSCLPCSLHDCFQTG*GPLCCSCKWWKC LQASSDPAPCHPCQSVWLTDWEGWHQDQGDPRD YGCPGTGGRGPAPQLHRASCYGIWGA*CHHPVC APDLRCYPGVPTQRSHYPLPSEPLPRYCSSLCQP GLLCPGSVWGCDPS*GHQAPAALKPCGPLCHTQ RGARTGSRHTDQLTGVLGSQRFDWLCDRAPGQQ DQRDPADVRGTYQDREPSRGRWGAACHHHWLSGL HRPGPVPHHCLSRDGQVYLWGDAQLGPRRPACP LLATPDGPAHSSPWPAGHTLCHLPLQLHRPQAH ALLGFTTCFPRAAAGLGGLHCQDGSS*WEQEG*A AEILPLLRPAEVQAGAGRTTSRGLPLHPTRPRR LHPGVPNAANAQTHGCTPYPASIYGSSFSQSGG SFWPRGLSCGSPRAGGPTSSALCLDTGSSQETP* CPHHGGCHSRTPHPLGLPGLLHPCGSQGGGPVG *LGPGFSPHHLQISCCFH*YPFDWNELAGLVRG HPKEGALPGSWGSGMGQGPSSQQQTLCTVFSIPV FE*IFSATR

Frame 1

CDLGQVTDPLQASGSPV**IHLLS*PVC*LSSS *AQYVLSTFSGLALN*ALGT*GEGSSLGSCSKG LLGLAVQRSRNQARHHKCFDGGSAGSWGNV*QGQ PA*GWPGCSESTVPASTAHLQPLESLWQNSNGR RSSCTQGSRVSPPLDRAGQVAARTHSQVAVAPA SPDRMSGSDGGLEEEPELSITLTLRMLMHGKEVG SIIGKKGETVKRIREQSSARITISEGSCPERIT TITGSTAAVFHAVSMIAFKLDEDLCAAPANGGN VSRPPVTLRLVIPASQCGSLIGKAGTKIKEIRET TGAQVQVAGDLLPNSTERAVTVSGVPDAIILCV RQICAVILESPPKGATIPYHPSLSLGTVLLSAN QGFSVQGQYGAVTPAEVTKLQQLSSHAVPFATPS VVPGLDPGTQTSSQEFLVPNDLIGCVIGRQGSK ISEIRQMSGAHIKIGNQAEGAGERHVTITGSPV SIALAQYLITACLETAKSTSGGTPSSAPADLPAP FSPPLTALPTAPPGLLGTPYAISLSNFIGLKPM PFLALPPASPGPPPGLAAYTAKMAAANGSKKAE RQKFSPY*GQLRYRQGQAGPPAGGCLCTLPAQGD STLGSQTPLTPRRMDAPPTLPPSMGVLSLRVGA VSGPGV*AAAAPGQGALPPQLCAWIQGAARRLP SAPTMAGVTHALPIP*GFLAYCILVGVREEGPLG SWGQASLPTTCRFLAASTDTLLTGMNWLGLSGG TPKRGHCQVAGGVAWGRGPVLSSRHSVQFFQSL FLNKYSQRPG

Frame 2

VTLGRLLILSRHQVLLCSEFICSANQYVS*VPA ELSMF*APSLAWL*TEPWGHKEKAAALAPAPRG SWAWLSREAETKHVITSALMGEVQAAGGMSSKG SQHEAGQDARRAQSLHPLLISSLWSPCGRTQMEG GAAAPRGAGCHLRWIEPARLQRGHTRRSLWPQP RLTE*AARTGDWRRSQSSASPSRCGC*CTGRKW AASSGRRARL*SESGSRAVPGSPSPRAPALNASP PSPGLQQLSSMQSP*LLSNWMRTFVLLLQMVEM SPGLQ*PCALSSLPVSVAH*LGRLAPRSRRSER LRVPRYRWQGTCSPTPQSELLRYLGCLMPSSCVC ARSALLSWSPHPKEPLSPTIRASP*VLFFSLPT RASLSRVSMGL*PQLRSPSSSSSQAMRSPLPHP AWCQDWIPAHRPAHRSSWFPTI*LAV*SGARAAR SARSGRCQGHISRSGTKQRALGSGMSPSLALRS PSPWPSTSSLPV*RRPSLPLGGRPARPPQTCLP PSRHP*RPCPQLPLACWAHPMPSPSPTSSASSPC PSWLYHLLPQGRRRAWRPTLPRWQQLMGARRLS GRNSPPTEAS*GTGRGRQDHQQGAASAPYPPKE TPPWGPKRR*RPDAWMHPLPCLHLWEFFLSEWGQ FLAQGSELRQPQGRGPYLLSSVLGYREQPGDSL VPPPWRVSLTHSPSLRASWPTASLWESGRRARW VAGARLLSPPPADFLLLPLIPF*LE*TGWACQGA PQRGGTAR*LGEWHGAGAQFSAADTLYSFFNPC F*INILSDQE

Sequence PCBP4.cAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGAGCG GCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAA GGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACA GCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGA CCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGTACA GGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCCTGTGTGTGCGCCAGATCTGCGCTGTT ATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGG GTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGA TCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAG ATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGT ACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCCCTTCTCGCCACCCCTGAC GGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCA CCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCT ACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCA AACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTC TGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGG GTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTC TCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCAC TGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAATAAATATTCTC AGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSESRPGCSADTLAGRCGPSLA*QNER LGRGTGGGARAQHHPHAADADAREGSGQHHREE GRDCKANPGAEQCPDHHLRGLLP*THHHHHRVY SSCLPCSLHDCFQTG*GPLCCSCKWWKCLQASSD PAPCHPCQSVWLTDWEGWHQDQGDPRDYGCPGT GGRGPAPQLHRASCYGIWGA*CHHPVCAPDLRC YPGVPTQRSHYPLPSEPLPRYCSSLCQPGLLCPG SVWGCDPS*GHQAPAALKPCGPLCHTQRGARTG SRHTDQLTGVLGSQRFDWLCDRAPGQQDQRDPA DVRGTYQDREPSRGRWGAACHHHWLSGLHRPGPV PHHCLSRDGQVYLWGDAQLGPRRPACPLLATPD GPAHSSPWPAGHTLCHLPLQLHRPQAHALLGFT TCFPRAAAGLGGLHCQDGSS*WEQEG*AAEILPL LRPAEVQAGAGRTTSRGLPLHPTRPRRLHPGVP NAANAQTHGCTPYPASIYGSSFSQSGGSFWPRG LSCGSPRAGGPTSSALCLDTGSSQETP*CPHHGG CHSRTPHPLGLPGLLHPCGSQGGGPVG*LGPGF SPHHLQISCCFH*YPFDWNELAGLVRGHPKEGA LPGSWGSGMGQGPSSQQQTLCTVFSIPVFE*IFS ATR

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPARAGQVAARTHSQVAVAPASPDRMS GSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKK GETVKRIREQSSARITISEGSCPERITTITGST AAVFHAVSMIAFKLDEDLCAAPANGGNVSRPPV TLRLVIPASQCGSLIGKAGTKIKEIRETTGAQVQ VAGDLLPNSTERAVTVSGVPDAIILCVRQICAV ILESPPKGATIPYHPSLSLGTVLLSANQGFSVQ GQYGAVTPAEVTKLQQLSSHAVPFATPSVVPGLD PGTQTSSQEFLVPNDLIGCVIGRQGSKISEIRQ MSGAHIKIGNQAEGAGERHVTITGSPVSIALAQ YLITACLETAKSTSGGTPSSAPADLPAPFSPPLT ALPTAPPGLLGTPYAISLSNFIGLKPMPFLALP PASPGPPPGLAAYTAKMAAANGSKKAERQKFSP Y*GQLRYRQGQAGPPAGGCLCTLPAQGDSTLGSQ TPLTPRRMDAPPTLPPSMGVLSLRVGAVSGPGV *AAAAPGQGALPPQLCAWIQGAARRLPSAPTMA GVTHALPIP*GFLAYCILVGVREEGPLGSWGQAS LPTTCRFLAASTDTLLTGMNWLGLSGGTPKRGH CQVAGGVAWGRGPVLSSRHSVQFFQSLFLNKYS QRPG

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQREPARLQRGHTRRSLWPQPRLTE*A ARTGDWRRSQSSASPSRCGC*CTGRKWAASSGR RARL*SESGSRAVPGSPSPRAPALNASPPSPGLQ QLSSMQSP*LLSNWMRTFVLLLQMVEMSPGLQ* PCALSSLPVSVAH*LGRLAPRSRRSERLRVPRY RWQGTCSPTPQSELLRYLGCLMPSSCVCARSALL SWSPHPKEPLSPTIRASP*VLFFSLPTRASLSR VSMGL*PQLRSPSSSSSQAMRSPLPHPAWCQDW IPAHRPAHRSSWFPTI*LAV*SGARAARSARSGR CQGHISRSGTKQRALGSGMSPSLALRSPSPWPS TSSLPV*RRPSLPLGGRPARPPQTCLPPSRHP* RPCPQLPLACWAHPMPSPSPTSSASSPCPSWLYH LLPQGRRRAWRPTLPRWQQLMGARRLSGRNSPP TEAS*GTGRGRQDHQQGAASAPYPPKETPPWGP KRR*RPDAWMHPLPCLHLWEFFLSEWGQFLAQGS ELRQPQGRGPYLLSSVLGYREQPGDSLVPPPWR VSLTHSPSLRASWPTASLWESGRRARWVAGARL LSPPPADFLLLPLIPF*LE*TGWACQGAPQRGGT AR*LGEWHGAGAQFSAADTLYSFFNPCF*INIL SDQE

Sequence PCBP4.eAug05

ATTTCTTTTAGTGTCTCAGGGTAAGGGAAAGGCTAGGTACCTACCATGTATGTGCTTATTGTTTTAATTCTCATCACTCTTTGGAGATGGGAATTTGTAT CCCCTTCTACAGATGGAGAAGCTGAGGCTCAGAGGGTTGAATGGGCTCCCCAGGCTTACACAGCTCGTGAGACACACATAAGCACCCTGGTCAGAGTGAT GTGTGGCGCTCAAGGTCCATGCAGTCTCTTTCCTCTGGGAGTTTGACTAGCCCAGCTCTGGGGTCCCCATGTAAGGGCAGGGGCAGGGTGGACTGGGCTC CTCTCGAACCCCTCTTTGGCTGCCCCTGCCAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGA GCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAA GAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCT ACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAG TGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGT ACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCCTGTGTGTGCGCCAGATCTGCGCT GTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCC AGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACT GGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGG CAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCC AGTACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCCCTTCTCGCCACCCCT GACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTA CCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCC CCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTC CCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGG GTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGG CGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGC TTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGG CACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAATAAATATT CTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

ISFSVSG*GKG*VPTMYVLIVLILITLWRWEFV SPSTDGEAEAQRVEWAPQAYTARETHISTLVRVM CGAQGPCSLFPLGV*LAQLWGPHVRAGAGWTGL LSNPSLAAPARAGQVAARTHSQVAVAPASPDRM SGSDGGLEEEPELSITLTLRMLMHGKEVGSIIGK KGETVKRIREQSSARITISEGSCPERITTITGS TAAVFHAVSMIAFKLDEDLCAAPANGGNVSRPP VTLRLVIPASQCGSLIGKAGTKIKEIRETTGAQV QVAGDLLPNSTERAVTVSGVPDAIILCVRQICA VILESPPKGATIPYHPSLSLGTVLLSANQGFSV QGQYGAVTPAEVTKLQQLSSHAVPFATPSVVPGL DPGTQTSSQEFLVPNDLIGCVIGRQGSKISEIR QMSGAHIKIGNQAEGAGERHVTITGSPVSIALA QYLITACLETAKSTSGGTPSSAPADLPAPFSPPL TALPTAPPGLLGTPYAISLSNFIGLKPMPFLAL PPASPGPPPGLAAYTAKMAAANGSKKAERQKFS PY*GQLRYRQGQAGPPAGGCLCTLPAQGDSTLGS QTPLTPRRMDAPPTLPPSMGVLSLRVGAVSGPG V*AAAAPGQGALPPQLCAWIQGAARRLPSAPTM AGVTHALPIP*GFLAYCILVGVREEGPLGSWGQA SLPTTCRFLAASTDTLLTGMNWLGLSGGTPKRG HCQVAGGVAWGRGPVLSSRHSVQFFQSLFLNKY SQRPG

Frame 1

FLLVSQGKGKARYLPCMCLLF*FSSLFGDGNLY PLLQMEKLRLRGLNGLPRLTQLVRHT*APWSE* CVALKVHAVSFLWEFD*PSSGVPM*GQGQGGLGS SRTPLWLPLPEPARLQRGHTRRSLWPQPRLTE* AARTGDWRRSQSSASPSRCGC*CTGRKWAASSG RRARL*SESGSRAVPGSPSPRAPALNASPPSPGL QQLSSMQSP*LLSNWMRTFVLLLQMVEMSPGLQ *PCALSSLPVSVAH*LGRLAPRSRRSERLRVPR YRWQGTCSPTPQSELLRYLGCLMPSSCVCARSAL LSWSPHPKEPLSPTIRASP*VLFFSLPTRASLS RVSMGL*PQLRSPSSSSSQAMRSPLPHPAWCQD WIPAHRPAHRSSWFPTI*LAV*SGARAARSARSG RCQGHISRSGTKQRALGSGMSPSLALRSPSPWP STSSLPV*RRPSLPLGGRPARPPQTCLPPSRHP *RPCPQLPLACWAHPMPSPSPTSSASSPCPSWLY HLLPQGRRRAWRPTLPRWQQLMGARRLSGRNSP PTEAS*GTGRGRQDHQQGAASAPYPPKETPPWG PKRR*RPDAWMHPLPCLHLWEFFLSEWGQFLAQG SELRQPQGRGPYLLSSVLGYREQPGDSLVPPPW RVSLTHSPSLRASWPTASLWESGRRARWVAGAR LLSPPPADFLLLPLIPF*LE*TGWACQGAPQRGG TAR*LGEWHGAGAQFSAADTLYSFFNPCF*INI LSDQE

Frame 2

FF*CLRVRERLGTYHVCAYCFNSHHSLEMGICI PFYRWRS*GSEG*MGSPGLHSS*DTHKHPGQSD VWRSRSMQSLSSGSLTSPALGSPCKGRGRVDWA PLEPLFGCPCQSRPGCSADTLAGRCGPSLA*QNE RLGRGTGGGARAQHHPHAADADAREGSGQHHRE EGRDCKANPGAEQCPDHHLRGLLP*THHHHHRV YSSCLPCSLHDCFQTG*GPLCCSCKWWKCLQASS DPAPCHPCQSVWLTDWEGWHQDQGDPRDYGCPG TGGRGPAPQLHRASCYGIWGA*CHHPVCAPDLR CYPGVPTQRSHYPLPSEPLPRYCSSLCQPGLLCP GSVWGCDPS*GHQAPAALKPCGPLCHTQRGART GSRHTDQLTGVLGSQRFDWLCDRAPGQQDQRDP ADVRGTYQDREPSRGRWGAACHHHWLSGLHRPGP VPHHCLSRDGQVYLWGDAQLGPRRPACPLLATP DGPAHSSPWPAGHTLCHLPLQLHRPQAHALLGF TTCFPRAAAGLGGLHCQDGSS*WEQEG*AAEILP LLRPAEVQAGAGRTTSRGLPLHPTRPRRLHPGV PNAANAQTHGCTPYPASIYGSSFSQSGGSFWPR GLSCGSPRAGGPTSSALCLDTGSSQETP*CPHHG GCHSRTPHPLGLPGLLHPCGSQGGGPVG*LGPG FSPHHLQISCCFH*YPFDWNELAGLVRGHPKEG ALPGSWGSGMGQGPSSQQQTLCTVFSIPVFE*IF SATR

Sequence PCBP4.fAug05

CTGTGACCTTGGGCAGGTTACTGATCCTCTCCAGGCATCAGGTTCTCCTGTGTAGTGAATTCATCTGCTCAGCTAACCAGTATGTTAGCTGAGTTCCAGC TGAGCTCAGTATGTTCTGAGCACCTTCTCTGGCCTGGCTCTGAACTGAGCCCTGGGGACATAAGGAGAAGGCAGCAGCCTTGGCTCCTGCTCCAAGGGGC TCCTGGGCTTGGCTGTCCAGAGAAGCAGAAACCAAGCACGTCATCACAAGTGCTTTGATGGGGGAAGTGCAGGCAGCTGGGGGAATGTCTAGCAAGGGCA GCCAGCATGAGGCTGGCCAGGATGCTCGGAGAGCACAGTCCCTGCATCCACTGCTCATCTCCAGCCTCTGGAGTCCCTGTGGCAGAACTCAAATGGAAGG AGGAGCAGCTGCACCCAGGGGAGCAGGGTGTCACCTCCGTTGGATAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCT CGCCTGACAGAATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGG CAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGGTGAGGATGGAGTTTGGGGGAGGAGCCCAGGGCACCTGATGTGGAGGGGAG ATCCCTGCCCCACTTTCTCTCTGAACCACTCCTTCTGGGCTGACCAGTGAGTCCCCCAGAGGGTGGCAGTTTTGAGCCTTGCCTCAGGCCTGGTGAGTGG GGCAGGCAGACAAGTTCCGGCAAGGGAGCAGGGAAGATGCCCCGCTGCCCGGCGCTCATTCTATATCTGCAGAGCAGTGCCCGGATCACCATCTCCGAGG GCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGC TGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACC AAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGTACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGC CTGATGCCATCATCCTGTGTGTGCGCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGG TACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCAT GCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTG TGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGT CACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCG GCCCCCGCAGACCTGCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCA ACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGC TAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTC TGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAG TTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACA GGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGA GTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGA ACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTC TGTACAGTTTTTTCAATCCCTGTTTTTGAATAAATATTCTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

L*PWAGY*SSPGIRFSCVVNSSAQLTSMLAEFQ LSSVCSEHLLWPGSELSPGDIRRRQQPWLLLQGA PGLGCPEKQKPSTSSQVL*WGKCRQLGECLARA ASMRLARMLGEHSPCIHCSSPASGVPVAELKWK EEQLHPGEQGVTSVG*SRPGCSADTLAGRCGPSL A*QNERLGRGTGGGARAQHHPHAADADAREGSG QHHREEGRDCKANPGAGEDGVWGRSPGHLMWRG DPCPTFSLNHSFWADQ*VPQRVAVLSLASGLVSG AGRQVPAREQGRCPAARRSFYICRAVPGSPSPR APALNASPPSPGLQQLSSMQSP*LLSNWMRTFV LLLQMVEMSPGLQ*PCALSSLPVSVAH*LGRLAP RSRRSERLRVPRYRWQGTCSPTPQSELLRYLGC LMPSSCVCARSALLSWSPHPKEPLSPTIRASP* VLFFSLPTRASLSRVSMGL*PQLRSPSSSSSQAM RSPLPHPAWCQDWIPAHRPAHRSSWFPTI*LAV *SGARAARSARSGRCQGHISRSGTKQRALGSGM SPSLALRSPSPWPSTSSLPV*RRPSLPLGGRPAR PPQTCLPPSRHP*RPCPQLPLACWAHPMPSPSP TSSASSPCPSWLYHLLPQGRRRAWRPTLPRWQQ LMGARRLSGRNSPPTEAS*GTGRGRQDHQQGAAS APYPPKETPPWGPKRR*RPDAWMHPLPCLHLWE FFLSEWGQFLAQGSELRQPQGRGPYLLSSVLGY REQPGDSLVPPPWRVSLTHSPSLRASWPTASLWE SGRRARWVAGARLLSPPPADFLLLPLIPF*LE* TGWACQGAPQRGGTAR*LGEWHGAGAQFSAADT LYSFFNPCF*INILSDQE

Frame 1

CDLGQVTDPLQASGSPV**IHLLS*PVC*LSSS *AQYVLSTFSGLALN*ALGT*GEGSSLGSCSKG LLGLAVQRSRNQARHHKCFDGGSAGSWGNV*QGQ PA*GWPGCSESTVPASTAHLQPLESLWQNSNGR RSSCTQGSRVSPPLDRAGQVAARTHSQVAVAPA SPDRMSGSDGGLEEEPELSITLTLRMLMHGKEVG SIIGKKGETVKRIREQVRMEFGGGAQGT*CGGE IPAPLSL*TTPSGLTSESPRGWQF*ALPQAW*V GQADKFRQGSREDAPLPGAHSISAEQCPDHHLRG LLP*THHHHHRVYSSCLPCSLHDCFQTG*GPLC CSCKWWKCLQASSDPAPCHPCQSVWLTDWEGWH QDQGDPRDYGCPGTGGRGPAPQLHRASCYGIWGA *CHHPVCAPDLRCYPGVPTQRSHYPLPSEPLPR YCSSLCQPGLLCPGSVWGCDPS*GHQAPAALKP CGPLCHTQRGARTGSRHTDQLTGVLGSQRFDWLC DRAPGQQDQRDPADVRGTYQDREPSRGRWGAAC HHHWLSGLHRPGPVPHHCLSRDGQVYLWGDAQL GPRRPACPLLATPDGPAHSSPWPAGHTLCHLPLQ LHRPQAHALLGFTTCFPRAAAGLGGLHCQDGSS *WEQEG*AAEILPLLRPAEVQAGAGRTTSRGLP LHPTRPRRLHPGVPNAANAQTHGCTPYPASIYGS SFSQSGGSFWPRGLSCGSPRAGGPTSSALCLDT GSSQETP*CPHHGGCHSRTPHPLGLPGLLHPCG SQGGGPVG*LGPGFSPHHLQISCCFH*YPFDWNE LAGLVRGHPKEGALPGSWGSGMGQGPSSQQQTL CTVFSIPVFE*IFSATR

Frame 2

VTLGRLLILSRHQVLLCSEFICSANQYVS*VPA ELSMF*APSLAWL*TEPWGHKEKAAALAPAPRG SWAWLSREAETKHVITSALMGEVQAAGGMSSKG SQHEAGQDARRAQSLHPLLISSLWSPCGRTQMEG GAAAPRGAGCHLRWIEPARLQRGHTRRSLWPQP RLTE*AARTGDWRRSQSSASPSRCGC*CTGRKW AASSGRRARL*SESGSR*GWSLGEEPRAPDVEGR SLPHFLSEPLLLG*PVSPPEGGSFEPCLRPGEW GRQTSSGKGAGKMPRCPALILYLQSSARITISE GSCPERITTITGSTAAVFHAVSMIAFKLDEDLCA APANGGNVSRPPVTLRLVIPASQCGSLIGKAGT KIKEIRETTGAQVQVAGDLLPNSTERAVTVSGV PDAIILCVRQICAVILESPPKGATIPYHPSLSLG TVLLSANQGFSVQGQYGAVTPAEVTKLQQLSSH AVPFATPSVVPGLDPGTQTSSQEFLVPNDLIGC VIGRQGSKISEIRQMSGAHIKIGNQAEGAGERHV TITGSPVSIALAQYLITACLETAKSTSGGTPSS APADLPAPFSPPLTALPTAPPGLLGTPYAISLS NFIGLKPMPFLALPPASPGPPPGLAAYTAKMAAA NGSKKAERQKFSPY*GQLRYRQGQAGPPAGGCL CTLPAQGDSTLGSQTPLTPRRMDAPPTLPPSMG VLSLRVGAVSGPGV*AAAAPGQGALPPQLCAWIQ GAARRLPSAPTMAGVTHALPIP*GFLAYCILVG VREEGPLGSWGQASLPTTCRFLAASTDTLLTGM NWLGLSGGTPKRGHCQVAGGVAWGRGPVLSSRHS VQFFQSLFLNKYSQRPG

Sequence PCBP4.gAug05

ATTTCTTTTAGTGTCTCAGGGTAAGGGAAAGGCTAGGTACCTACCATGTATGTGCTTATTGTTTTAATTCTCATCACTCTTTGGAGATGGGAATTTGTAT CCCCTTCTACAGATGGAGAAGCTGAGGCTCAGAGGGTTGAATGGGCTCCCCAGGCTTACACAGCTCGTGAGACACACATAAGCACCCTGGTCAGAGTGAT GTGTGGCGCTCAAGGTCCATGCAGTCTCTTTCCTCTGGGAGTTTGACTAGCCCAGCTCTGGGGTCCCCATGTAAGGGCAGGGGCAGGGTGGACTGGGCTC CTCTCGAACCCCTCTTTGGCTGCCCCTGCCAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGA GCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAA GAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGGTGAGGATGGAGTTTGGGGGAGGAGCCCAGGGCACCTGATGTGGAGGGGAGATCCCTGCCCCACTT TCTCTCTGAACCACTCCTTCTGGGCTGACCAGTGAGTCCCCCAGAGGGTGGCAGTTTTGAGCCTTGCCTCAGGCCTGGTGAGTGGGGCAGGCAGACAAGT TCCGGCAAGGGAGCAGGGAAGATGCCCCGCTGCCCGGCGCTCATTCTATATCTGCAGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAAC GCATCACCACCATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGG TGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATC CGAGAGACTACGGGTGCCCAGGTACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCC TGTGTGTGCGCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTC TGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCC ACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGG GCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTC TCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTG CCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCA AGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAA GGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCC CAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAG TGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAG ACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGC CCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGT CAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCA ATCCCTGTTTTTGAATAAATATTCTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

ISFSVSG*GKG*VPTMYVLIVLILITLWRWEFV SPSTDGEAEAQRVEWAPQAYTARETHISTLVRVM CGAQGPCSLFPLGV*LAQLWGPHVRAGAGWTGL LSNPSLAAPARAGQVAARTHSQVAVAPASPDRM SGSDGGLEEEPELSITLTLRMLMHGKEVGSIIGK KGETVKRIREQVRMEFGGGAQGT*CGGEIPAPL SL*TTPSGLTSESPRGWQF*ALPQAW*VGQADK FRQGSREDAPLPGAHSISAEQCPDHHLRGLLP*T HHHHHRVYSSCLPCSLHDCFQTG*GPLCCSCKW WKCLQASSDPAPCHPCQSVWLTDWEGWHQDQGD PRDYGCPGTGGRGPAPQLHRASCYGIWGA*CHHP VCAPDLRCYPGVPTQRSHYPLPSEPLPRYCSSL CQPGLLCPGSVWGCDPS*GHQAPAALKPCGPLC HTQRGARTGSRHTDQLTGVLGSQRFDWLCDRAPG QQDQRDPADVRGTYQDREPSRGRWGAACHHHWL SGLHRPGPVPHHCLSRDGQVYLWGDAQLGPRRP ACPLLATPDGPAHSSPWPAGHTLCHLPLQLHRPQ AHALLGFTTCFPRAAAGLGGLHCQDGSS*WEQE G*AAEILPLLRPAEVQAGAGRTTSRGLPLHPTR PRRLHPGVPNAANAQTHGCTPYPASIYGSSFSQS GGSFWPRGLSCGSPRAGGPTSSALCLDTGSSQE TP*CPHHGGCHSRTPHPLGLPGLLHPCGSQGGG PVG*LGPGFSPHHLQISCCFH*YPFDWNELAGLV RGHPKEGALPGSWGSGMGQGPSSQQQTLCTVFS IPVFE*IFSATR

Frame 1

FLLVSQGKGKARYLPCMCLLF*FSSLFGDGNLY PLLQMEKLRLRGLNGLPRLTQLVRHT*APWSE* CVALKVHAVSFLWEFD*PSSGVPM*GQGQGGLGS SRTPLWLPLPEPARLQRGHTRRSLWPQPRLTE* AARTGDWRRSQSSASPSRCGC*CTGRKWAASSG RRARL*SESGSR*GWSLGEEPRAPDVEGRSLPHF LSEPLLLG*PVSPPEGGSFEPCLRPGEWGRQTS SGKGAGKMPRCPALILYLQSSARITISEGSCPE RITTITGSTAAVFHAVSMIAFKLDEDLCAAPANG GNVSRPPVTLRLVIPASQCGSLIGKAGTKIKEI RETTGAQVQVAGDLLPNSTERAVTVSGVPDAII LCVRQICAVILESPPKGATIPYHPSLSLGTVLLS ANQGFSVQGQYGAVTPAEVTKLQQLSSHAVPFA TPSVVPGLDPGTQTSSQEFLVPNDLIGCVIGRQ GSKISEIRQMSGAHIKIGNQAEGAGERHVTITGS PVSIALAQYLITACLETAKSTSGGTPSSAPADL PAPFSPPLTALPTAPPGLLGTPYAISLSNFIGL KPMPFLALPPASPGPPPGLAAYTAKMAAANGSKK AERQKFSPY*GQLRYRQGQAGPPAGGCLCTLPA QGDSTLGSQTPLTPRRMDAPPTLPPSMGVLSLR VGAVSGPGV*AAAAPGQGALPPQLCAWIQGAARR LPSAPTMAGVTHALPIP*GFLAYCILVGVREEG PLGSWGQASLPTTCRFLAASTDTLLTGMNWLGL SGGTPKRGHCQVAGGVAWGRGPVLSSRHSVQFFQ SLFLNKYSQRPG

Frame 2

FF*CLRVRERLGTYHVCAYCFNSHHSLEMGICI PFYRWRS*GSEG*MGSPGLHSS*DTHKHPGQSD VWRSRSMQSLSSGSLTSPALGSPCKGRGRVDWA PLEPLFGCPCQSRPGCSADTLAGRCGPSLA*QNE RLGRGTGGGARAQHHPHAADADAREGSGQHHRE EGRDCKANPGAGEDGVWGRSPGHLMWRGDPCPT FSLNHSFWADQ*VPQRVAVLSLASGLVSGAGRQV PAREQGRCPAARRSFYICRAVPGSPSPRAPALN ASPPSPGLQQLSSMQSP*LLSNWMRTFVLLLQM VEMSPGLQ*PCALSSLPVSVAH*LGRLAPRSRRS ERLRVPRYRWQGTCSPTPQSELLRYLGCLMPSS CVCARSALLSWSPHPKEPLSPTIRASP*VLFFS LPTRASLSRVSMGL*PQLRSPSSSSSQAMRSPLP HPAWCQDWIPAHRPAHRSSWFPTI*LAV*SGAR AARSARSGRCQGHISRSGTKQRALGSGMSPSLA LRSPSPWPSTSSLPV*RRPSLPLGGRPARPPQTC LPPSRHP*RPCPQLPLACWAHPMPSPSPTSSAS SPCPSWLYHLLPQGRRRAWRPTLPRWQQLMGAR RLSGRNSPPTEAS*GTGRGRQDHQQGAASAPYPP KETPPWGPKRR*RPDAWMHPLPCLHLWEFFLSE WGQFLAQGSELRQPQGRGPYLLSSVLGYREQPG DSLVPPPWRVSLTHSPSLRASWPTASLWESGRRA RWVAGARLLSPPPADFLLLPLIPF*LE*TGWAC QGAPQRGGTAR*LGEWHGAGAQFSAADTLYSFF NPCF*INILSDQE

Sequence PCBP4.hAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGGCCATTTCCAGCACATAGAAGAGAGATTGGAAACCAACGTGCAGAACTGCCAGTCCCCTGACACGCTGTGC CCCACCCACTGCAGCCCAGTGCTGAATGAACCCTGCCCAGAGGTGTCTGTAGTGAGCTTCTGCCCTAGTGACTTTTGAGCCGGCCAGGTTGCAGCGCGGA CACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCT GCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGAGCAGTGCCCGGATCACCATC TCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACC TTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGC TGGCACCAAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGTACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCT GGGGTGCCTGATGCCATCATCCTGTGTGTGCGCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCT CCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTC AAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATT GGCTGTGTGATCGGGCGCCAGGGCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGC GGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGA TGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGG GGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCA TCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTG CTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCAT CCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTG ACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCA GCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAATAAATATTCTCAGCGACCAGGAA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSEAISST*KRDWKPTCRTASPLTRCA PPTAAQC*MNPAQRCL**ASALVTFEPARLQRG HTRRSLWPQPRLTE*AARTGDWRRSQSSASPSR CGC*CTGRKWAASSGRRARL*SESGSRAVPGSPS PRAPALNASPPSPGLQQLSSMQSP*LLSNWMRT FVLLLQMVEMSPGLQ*PCALSSLPVSVAH*LGR LAPRSRRSERLRVPRYRWQGTCSPTPQSELLRYL GCLMPSSCVCARSALLSWSPHPKEPLSPTIRAS P*VLFFSLPTRASLSRVSMGL*PQLRSPSSSSS QAMRSPLPHPAWCQDWIPAHRPAHRSSWFPTI*L AV*SGARAARSARSGRCQGHISRSGTKQRALGS GMSPSLALRSPSPWPSTSSLPGPPPGLAAYTAK MAAANGSKKAERQKFSPY*GQLRYRQGQAGPPAG GCLCTLPAQGDSTLGSQTPLTPRRMDAPPTLPP SMGVLSLRVGAVSGPGV*AAAAPGQGALPPQLC AWIQGAARRLPSAPTMAGVTHALPIP*GFLAYCI LVGVREEGPLGSWGQASLPTTCRFLAASTDTLL TGMNWLGLSGGTPKRGHCQVAGGVAWGRGPVLS SRHSVQFFQSLFLNKYSQRPG

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPARPFPAHRREIGNQRAELPVP*HAV PHPLQPSAE*TLPRGVCSELLP**LLSRPGCSAD TLAGRCGPSLA*QNERLGRGTGGGARAQHHPHA ADADAREGSGQHHREEGRDCKANPGAEQCPDHH LRGLLP*THHHHHRVYSSCLPCSLHDCFQTG*GP LCCSCKWWKCLQASSDPAPCHPCQSVWLTDWEG WHQDQGDPRDYGCPGTGGRGPAPQLHRASCYGI WGA*CHHPVCAPDLRCYPGVPTQRSHYPLPSEPL PRYCSSLCQPGLLCPGSVWGCDPS*GHQAPAAL KPCGPLCHTQRGARTGSRHTDQLTGVLGSQRFD WLCDRAPGQQDQRDPADVRGTYQDREPSRGRWGA ACHHHWLSGLHRPGPVPHHCLGRRRAWRPTLPR WQQLMGARRLSGRNSPPTEAS*GTGRGRQDHQQ GAASAPYPPKETPPWGPKRR*RPDAWMHPLPCLH LWEFFLSEWGQFLAQGSELRQPQGRGPYLLSSV LGYREQPGDSLVPPPWRVSLTHSPSLRASWPTA SLWESGRRARWVAGARLLSPPPADFLLLPLIPF* LE*TGWACQGAPQRGGTAR*LGEWHGAGAQFSA ADTLYSFFNPCF*INILSDQE

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQRGHFQHIEERLETNVQNCQSPDTLC PTHCSPVLNEPCPEVSVVSFCPSDF*AGQVAAR THSQVAVAPASPDRMSGSDGGLEEEPELSITLTL RMLMHGKEVGSIIGKKGETVKRIREQSSARITI SEGSCPERITTITGSTAAVFHAVSMIAFKLDED LCAAPANGGNVSRPPVTLRLVIPASQCGSLIGKA GTKIKEIRETTGAQVQVAGDLLPNSTERAVTVS GVPDAIILCVRQICAVILESPPKGATIPYHPSL SLGTVLLSANQGFSVQGQYGAVTPAEVTKLQQLS SHAVPFATPSVVPGLDPGTQTSSQEFLVPNDLI GCVIGRQGSKISEIRQMSGAHIKIGNQAEGAGE RHVTITGSPVSIALAQYLITAWAAAGLGGLHCQD GSS*WEQEG*AAEILPLLRPAEVQAGAGRTTSR GLPLHPTRPRRLHPGVPNAANAQTHGCTPYPAS IYGSSFSQSGGSFWPRGLSCGSPRAGGPTSSALC LDTGSSQETP*CPHHGGCHSRTPHPLGLPGLLH PCGSQGGGPVG*LGPGFSPHHLQISCCFH*YPF DWNELAGLVRGHPKEGALPGSWGSGMGQGPSSQQ QTLCTVFSIPVFE*IFSATR

Sequence PCBP4.lAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCTGTGGCCCCAGCCTCGCCTGACAGAATGAGCG GCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACGGGAAGGAAGTGGGCAGCATCATCGGGAAGAA GGGCGAGACTGTAAAGCGAATCCGGGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAACGCATCACCACCATCACCGGGTCTACAGCAGC TGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGAAATGTCTCCAGGCCTCCAGTGACCCTG CGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATCCGAGAGACTACGGGTGCCCAGGTACAGGTGG CAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCCTGTGTGTGCGCCAGATCTGCGCTGTTATCCT GGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTCTGCCAACCAGGGCTTCTCTGTCCAGGGTCAG TATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCCACACCCAGCGTGGTGCCAGGACTGGATCCCG GCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATGTGAGCAGGGGATGGGATGGCTGGAGGGGCAGCAAGATCAGCGAGATCCGGCAGATGTC AGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTCTCCGGTCTCCATCGCCCTGGCCCAGTACCTC ATCACTGCCTGGTGAGCGCGGGCTGGGCGGCAGTGGGGGAGCAGGTCACGGTTCTCATGTGCCCAAGAAAGGCAGGGGTGGGGAGAGGAAGCTGGCCTCC TCTCTCTGTCTGGGCCCGACCTCTGCCTCTCCTAACCCTACTCCAATTCCCCATGGTCTTTGCCTAATTCACCCTCTGTTGCCCCATCTCCCCCCTCTAT ATCCACCTCTCATTCTCCATTGCTGTGTCTTTTCCCTGGGTCTCTGGCCACCCCATTTCTCCCTGCACCTCGTGCTATATCTGCTTGTCCTTTCTTCCCT TCCTCTTCCACCTTTCCCATCTTCCCCTTATTGTTCTCTGTTCACTACCTCTCTCTTGCCTTTCATCTAATCTCATGCCCATCTCTGCCCTCATTGCCCC TCTCTCACTCCCACTTTCCCCTTTGTCTCCCCTCTATATCCCTCTCTCCAGTCTAGAGACGGCCAAGTCTACCTCTGGGGGGACGCCCAGCTCGGCCCCC GCAGACCTGCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGCACACCCTATGCCATCTCCCTCTCCAACTTCA TCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGGCCTACACTGCCAAGATGGCAGCAGCTAATGG GAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGCAGGACCACCAGCAGGGGGCTGCCTCTGCACC CTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCCCCTACCCTGCCTCCATCTATGGGAGTTCTTT CTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTACCTCCTCAGCTCTGTGCTTGGATACAGGGAGC AGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTTCCTGGCCTACTGCATCCTTGTGGGAGTCAGG GAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCCACTGATACCCTTTTGACTGGAATGAACTGGC TGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGGGCCCAGTTCTCAGCAGCAGACACTCTGTACA GTTTTTTCAATCCCTGTTTTTGAATA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSESRPGCSADTLAGRCGPSLA*QNER LGRGTGGGARAQHHPHAADADAREGSGQHHREE GRDCKANPGAVPGSPSPRAPALNASPPSPGLQQ LSSMQSP*LLSNWMRTFVLLLQMVEMSPGLQ*PC ALSSLPVSVAH*LGRLAPRSRRSERLRVPRYRW QGTCSPTPQSELLRYLGCLMPSSCVCARSALLS WSPHPKEPLSPTIRASP*VLFFSLPTRASLSRVS MGL*PQLRSPSSSSSQAMRSPLPHPAWCQDWIP AHRPAHRSSWFPTM*AGDGMAGGAARSARSGRC QGHISRSGTKQRALGSGMSPSLALRSPSPWPSTS SLPGERGLGGSGGAGHGSHVPKKGRGGERKLAS SLCLGPTSASPNPTPIPHGLCLIHPLLPHLPPL YPPLILHCCVFSLGLWPPHFSLHLVLYLLVLSSL PLPPFPSSPYCSLFTTSLLPFI*SHAHLCPHCP SLTPTFPFVSPLYPSLQSRDGQVYLWGDAQLGP RRPACPLLATPDGPAHSSPWPAGHTLCHLPLQLH RPQAHALLGFTTCFPRAAAGLGGLHCQDGSS*W EQEG*AAEILPLLRPAEVQAGAGRTTSRGLPLH PTRPRRLHPGVPNAANAQTHGCTPYPASIYGSSF SQSGGSFWPRGLSCGSPRAGGPTSSALCLDTGS SQETP*CPHHGGCHSRTPHPLGLPGLLHPCGSQ GGGPVG*LGPGFSPHHLQISCCFH*YPFDWNELA GLVRGHPKEGALPGSWGSGMGQGPSSQQQTLCT VFSIPVFE

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPARAGQVAARTHSQVAVAPASPDRMS GSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKK GETVKRIREQCPDHHLRGLLP*THHHHHRVYSS CLPCSLHDCFQTG*GPLCCSCKWWKCLQASSDP APCHPCQSVWLTDWEGWHQDQGDPRDYGCPGTGG RGPAPQLHRASCYGIWGA*CHHPVCAPDLRCYP GVPTQRSHYPLPSEPLPRYCSSLCQPGLLCPGS VWGCDPS*GHQAPAALKPCGPLCHTQRGARTGSR HTDQLTGVLGSQRCEQGMGWLEGQQDQRDPADV RGTYQDREPSRGRWGAACHHHWLSGLHRPGPVP HHCLVSAGWAAVGEQVTVLMCPRKAGVGRGSWPP LSVWARPLPLLTLLQFPMVFA*FTLCCPISPLY IHLSFSIAVSFPWVSGHPISPCTSCYICLSFLP FLFHLSHLPLIVLCSLPLSCLSSNLMPISALIAP LSLPLSPLSPLYIPLSSLETAKSTSGGTPSSAP ADLPAPFSPPLTALPTAPPGLLGTPYAISLSNF IGLKPMPFLALPPASPGPPPGLAAYTAKMAAANG SKKAERQKFSPY*GQLRYRQGQAGPPAGGCLCT LPAQGDSTLGSQTPLTPRRMDAPPTLPPSMGVL SLRVGAVSGPGV*AAAAPGQGALPPQLCAWIQGA ARRLPSAPTMAGVTHALPIP*GFLAYCILVGVR EEGPLGSWGQASLPTTCRFLAASTDTLLTGMNW LGLSGGTPKRGHCQVAGGVAWGRGPVLSSRHSVQ FFQSLFLN

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQREPARLQRGHTRRSLWPQPRLTE*A ARTGDWRRSQSSASPSRCGC*CTGRKWAASSGR RARL*SESGSSARITISEGSCPERITTITGSTAA VFHAVSMIAFKLDEDLCAAPANGGNVSRPPVTL RLVIPASQCGSLIGKAGTKIKEIRETTGAQVQV AGDLLPNSTERAVTVSGVPDAIILCVRQICAVIL ESPPKGATIPYHPSLSLGTVLLSANQGFSVQGQ YGAVTPAEVTKLQQLSSHAVPFATPSVVPGLDP GTQTSSQEFLVPNDVSRGWDGWRGSKISEIRQMS GAHIKIGNQAEGAGERHVTITGSPVSIALAQYL ITAW*ARAGRQWGSRSRFSCAQERQGWGEEAGL LSLSGPDLCLS*PYSNSPWSLPNSPSVAPSPPSI STSHSPLLCLFPGSLATPFLPAPRAISACPFFP SSSTFPIFPLLFSVHYLSLAFHLISCPSLPSLP LSHSHFPLCLPSISLSPV*RRPSLPLGGRPARPP QTCLPPSRHP*RPCPQLPLACWAHPMPSPSPTS SASSPCPSWLYHLLPQGRRRAWRPTLPRWQQLM GARRLSGRNSPPTEAS*GTGRGRQDHQQGAASAP YPPKETPPWGPKRR*RPDAWMHPLPCLHLWEFF LSEWGQFLAQGSELRQPQGRGPYLLSSVLGYRE QPGDSLVPPPWRVSLTHSPSLRASWPTASLWESG RRARWVAGARLLSPPPADFLLLPLIPF*LE*TG WACQGAPQRGGTAR*LGEWHGAGAQFSAADTLY SFFNPCF*I

Sequence PCBP4.mAug05

AAACCCCTCATTGTTCGCAGCTGATGTCACTCGCAGTTGTGAGCGGCCGCCTCTCCCGGGGACAATGTGGGACTGAGCGGCCCAGCCGCCGTGCCGCCGC CGCCGCCGCCGCAGGACAGCCCCAGCGAGCCTCTGGAGTCCCTGTGGCAGAACTCAAATGGAAGGAGGAGCAGCTGCACCCAGGGGAGCAGGGTGTCACC TCCGTTGGATAGGCCATTTCCAGCACATAGAAGAGAGATTGGAAACCAACGTGCAGAACTGCCAGTCCCCTGACACGCTGTGCCCCACCCACTGCAGCCC AGTGCTGAATGAACCCTGCCCAGAGGTGTCTGTAGTGAGCTTCTGCCCTAGTGACTTTTGAGCCGGCCAGGTTGCAGCGCGGACACACTCGCAGGTCGCT GTGGCCCCAGCCTCGCCTGACAGAATGAGCGGCTCGGACGGGGGACTGGAGGAGGAGCCAGAGCTCAGCATCACCCTCACGCTGCGGATGCTGATGCACG GGAAGGAAGTGGGCAGCATCATCGGGAAGAAGGGCGAGACTGTAAAGCGAATCCGGGAGCAGTGCCCGGATCACCATCTCCGAGGGCTCCTGCCCTGAAC GCATCACCACCATCACCGGGTCTACAGCAGCTGTCTTCCATGCAGTCTCCATGATTGCTTTCAAACTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGG TGGAAATGTCTCCAGGCCTCCAGTGACCCTGCGCCTTGTCATCCCTGCCAGTCAGTGTGGCTCACTGATTGGGAAGGCTGGCACCAAGATCAAGGAGATC CGAGAGACTACGGGTGCCCAGGTACAGGTGGCAGGGGACCTGCTCCCCAACTCCACAGAGCGAGCTGTTACGGTATCTGGGGTGCCTGATGCCATCATCC TGTGTGTGCGCCAGATCTGCGCTGTTATCCTGGAGTCCCCACCCAAAGGAGCCACTATCCCCTACCATCCGAGCCTCTCCCTAGGTACTGTTCTTCTCTC TGCCAACCAGGGCTTCTCTGTCCAGGGTCAGTATGGGGCTGTGACCCCAGCTGAGGTCACCAAGCTCCAGCAGCTCTCAAGCCATGCGGTCCCCTTTGCC ACACCCAGCGTGGTGCCAGGACTGGATCCCGGCACACAGACCAGCTCACAGGAGTTCTTGGTTCCCAACGATTTGATTGGCTGTGTGATCGGGCGCCAGG GCAGCAAGATCAGCGAGATCCGGCAGATGTCAGGGGCACATATCAAGATCGGGAACCAAGCAGAGGGCGCTGGGGAGCGGCATGTCACCATCACTGGCTC TCCGGTCTCCATCGCCCTGGCCCAGTACCTCATCACTGCCTGGTGAGCGCGGGCTGGGCGGCAGTGGGGGAGCAGGTCACGGTTCTCATGTGCCCAAGAA AGGCAGGGGTGGGGAGAGGAAGCTGGCCTCCTCTCTCTGTCTGGGCCCGACCTCTGCCTCTCCTAACCCTACTCCAATTCCCCATGGTCTTTGCCTAATT CACCCTCTGTTGCCCCATCTCCCCCCTCTATATCCACCTCTCATTCTCCATTGCTGTGTCTTTTCCCTGGGTCTCTGGCCACCCCATTTCTCCCTGCACC TCGTGCTATATCTGCTTGTCCTTTCTTCCCTTCCTCTTCCACCTTTCCCATCTTCCCCTTATTGTTCTCTGTTCACTACCTCTCTCTTGCCTTTCATCTA ATCTCATGCCCATCTCTGCCCTCATTGCCCCTCTCTCACTCCCACTTTCCCCTTTGTCTCCCCTCTATATCCCTCTCTCCAGTCTAGAGACGGCCAAGTC TACCTCTGGGGGGACGCCCAGCTCGGCCCCCGCAGACCTGCCTGCCCCCTTCTCGCCACCCCTGACGGCCCTGCCCACAGCTCCCCCTGGCCTGCTGGGC ACACCCTATGCCATCTCCCTCTCCAACTTCATCGGCCTCAAGCCCATGCCCTTCTTGGCTTTACCACCTGCTTCCCCAGGGCCGCCGCCGGGCTTGGCGG CCTACACTGCCAAGATGGCAGCAGCTAATGGGAGCAAGAAGGCTGAGCGGCAGAAATTCTCCCCCTACTGAGGCCAGCTGAGGTACAGGCAGGGGCAGGC AGGACCACCAGCAGGGGGCTGCCTCTGCACCCTACCCGCCCAAGGAGACTCCACCCTGGGGTCCCAAACGCCGCTAACGCCCAGACGCATGGATGCACCC CCTACCCTGCCTCCATCTATGGGAGTTCTTTCTCTCAGAGTGGGGGCAGTTTCTGGCCCAGGGGTCTGAGCTGCGGCAGCCCCAGGGCAGGGGGCCCTAC CTCCTCAGCTCTGTGCTTGGATACAGGGAGCAGCCAGGAGACTCCCTAGTGCCCCCACCATGGCGGGTGTCACTCACGCACTCCCCATCCCTTAGGGCTT CCTGGCCTACTGCATCCTTGTGGGAGTCAGGGAGGAGGGCCCGTTGGGTAGCTGGGGCCAGGCTTCTCTCCCCACCACCTGCAGATTTCTTGCTGCTTCC ACTGATACCCTTTTGACTGGAATGAACTGGCTGGGCTTGTCAGGGGGCACCCCAAAGAGGGGGCACTGCCAGGTAGCTGGGGGAGTGGCATGGGGCAGGG GCCCAGTTCTCAGCAGCAGACACTCTGTACAGTTTTTTCAATCCCTGTTTTTGAATA

PCBP4-3 (forward primer)
PCBP4-4 (reverse primer)
Single exon skipping

Frame 0

KPLIVRS*CHSQL*AAASPGDNVGLSGPAAVPP PPPPQDSPSEPLESLWQNSNGRRSSCTQGSRVSP PLDRPFPAHRREIGNQRAELPVP*HAVPHPLQP SAE*TLPRGVCSELLP**LLSRPGCSADTLAGR CGPSLA*QNERLGRGTGGGARAQHHPHAADADAR EGSGQHHREEGRDCKANPGAVPGSPSPRAPALN ASPPSPGLQQLSSMQSP*LLSNWMRTFVLLLQM VEMSPGLQ*PCALSSLPVSVAH*LGRLAPRSRRS ERLRVPRYRWQGTCSPTPQSELLRYLGCLMPSS CVCARSALLSWSPHPKEPLSPTIRASP*VLFFS LPTRASLSRVSMGL*PQLRSPSSSSSQAMRSPLP HPAWCQDWIPAHRPAHRSSWFPTI*LAV*SGAR AARSARSGRCQGHISRSGTKQRALGSGMSPSLA LRSPSPWPSTSSLPGERGLGGSGGAGHGSHVPKK GRGGERKLASSLCLGPTSASPNPTPIPHGLCLI HPLLPHLPPLYPPLILHCCVFSLGLWPPHFSLH LVLYLLVLSSLPLPPFPSSPYCSLFTTSLLPFI* SHAHLCPHCPSLTPTFPFVSPLYPSLQSRDGQV YLWGDAQLGPRRPACPLLATPDGPAHSSPWPAG HTLCHLPLQLHRPQAHALLGFTTCFPRAAAGLGG LHCQDGSS*WEQEG*AAEILPLLRPAEVQAGAG RTTSRGLPLHPTRPRRLHPGVPNAANAQTHGCT PYPASIYGSSFSQSGGSFWPRGLSCGSPRAGGPT SSALCLDTGSSQETP*CPHHGGCHSRTPHPLGL PGLLHPCGSQGGGPVG*LGPGFSPHHLQISCCF H*YPFDWNELAGLVRGHPKEGALPGSWGSGMGQG PSSQQQTLCTVFSIPVFE

Frame 1

NPSLFAADVTRSCERPPLPGTMWD*AAQPPCRR RRRRRTAPASLWSPCGRTQMEGGAAAPRGAGCH LRWIGHFQHIEERLETNVQNCQSPDTLCPTHCSP VLNEPCPEVSVVSFCPSDF*AGQVAARTHSQVA VAPASPDRMSGSDGGLEEEPELSITLTLRMLMH GKEVGSIIGKKGETVKRIREQCPDHHLRGLLP*T HHHHHRVYSSCLPCSLHDCFQTG*GPLCCSCKW WKCLQASSDPAPCHPCQSVWLTDWEGWHQDQGD PRDYGCPGTGGRGPAPQLHRASCYGIWGA*CHHP VCAPDLRCYPGVPTQRSHYPLPSEPLPRYCSSL CQPGLLCPGSVWGCDPS*GHQAPAALKPCGPLC HTQRGARTGSRHTDQLTGVLGSQRFDWLCDRAPG QQDQRDPADVRGTYQDREPSRGRWGAACHHHWL SGLHRPGPVPHHCLVSAGWAAVGEQVTVLMCPR KAGVGRGSWPPLSVWARPLPLLTLLQFPMVFA*F TLCCPISPLYIHLSFSIAVSFPWVSGHPISPCT SCYICLSFLPFLFHLSHLPLIVLCSLPLSCLSS NLMPISALIAPLSLPLSPLSPLYIPLSSLETAKS TSGGTPSSAPADLPAPFSPPLTALPTAPPGLLG TPYAISLSNFIGLKPMPFLALPPASPGPPPGLA AYTAKMAAANGSKKAERQKFSPY*GQLRYRQGQA GPPAGGCLCTLPAQGDSTLGSQTPLTPRRMDAP PTLPPSMGVLSLRVGAVSGPGV*AAAAPGQGAL PPQLCAWIQGAARRLPSAPTMAGVTHALPIP*GF LAYCILVGVREEGPLGSWGQASLPTTCRFLAAS TDTLLTGMNWLGLSGGTPKRGHCQVAGGVAWGR GPVLSSRHSVQFFQSLFLN

Frame 2

TPHCSQLMSLAVVSGRLSRGQCGTERPSRRAAA AAAAGQPQRASGVPVAELKWKEEQLHPGEQGVT SVG*AISST*KRDWKPTCRTASPLTRCAPPTAA QC*MNPAQRCL**ASALVTFEPARLQRGHTRRSL WPQPRLTE*AARTGDWRRSQSSASPSRCGC*CT GRKWAASSGRRARL*SESGSSARITISEGSCPE RITTITGSTAAVFHAVSMIAFKLDEDLCAAPANG GNVSRPPVTLRLVIPASQCGSLIGKAGTKIKEI RETTGAQVQVAGDLLPNSTERAVTVSGVPDAII LCVRQICAVILESPPKGATIPYHPSLSLGTVLLS ANQGFSVQGQYGAVTPAEVTKLQQLSSHAVPFA TPSVVPGLDPGTQTSSQEFLVPNDLIGCVIGRQ GSKISEIRQMSGAHIKIGNQAEGAGERHVTITGS PVSIALAQYLITAW*ARAGRQWGSRSRFSCAQE RQGWGEEAGLLSLSGPDLCLS*PYSNSPWSLPN SPSVAPSPPSISTSHSPLLCLFPGSLATPFLPAP RAISACPFFPSSSTFPIFPLLFSVHYLSLAFHL ISCPSLPSLPLSHSHFPLCLPSISLSPV*RRPS LPLGGRPARPPQTCLPPSRHP*RPCPQLPLACWA HPMPSPSPTSSASSPCPSWLYHLLPQGRRRAWR PTLPRWQQLMGARRLSGRNSPPTEAS*GTGRGR QDHQQGAASAPYPPKETPPWGPKRR*RPDAWMHP LPCLHLWEFFLSEWGQFLAQGSELRQPQGRGPY LLSSVLGYREQPGDSLVPPPWRVSLTHSPSLRA SWPTASLWESGRRARWVAGARLLSPPPADFLLLP LIPF*LE*TGWACQGAPQRGGTAR*LGEWHGAG AQFSAADTLYSFFNPCF*I