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Sequences

Sequence BCLAF1.bAug05

ACGCCCCAGATCGGAAGTGACGGAGACGTGCTAGCGCGTCGAAGGTAGCTCTATGGTTTTCCTCGCGTTCTTGAGTCGGGAAATGGCCGCTGTGTGGTTG CAACGGAGATAAATTCCCGGAACCGCGATTCGGCGTGTCAGGAATTCGAATTTAGAGTTTAATTTCTCAGAGCATTCTCTCCAGGAAGAATTTTTACAGT ATCTCAAAGACTTCACTTGACTTCTTGATCCTGCATAAAACCAAGGAGAAAAGAAATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCT AGATCACAGTCTAGTTCTCGATCAAGATCAAGATCTCATTCTAGAAAGAAGCGATACAGGTCTCGTTCCAGAACATATTCAAGGTCTCGTAGTAGAGATC GTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGG AGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCAAAAAGA AGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGATCATCCTCTTCTCGTTCTTCATCCCCATATA GCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAAAACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAAGTAAATCACA GGAGGAACCGAAAGATACATTTGAACATGACCCATCTGAGTCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGTGATATTTGGCCTGGCCTTTCA GCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTACACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATGCTCAGTACAG TTCACTCTGCAAAAAATACTCCTTCTCAGCATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGATCTAGTCGATA CAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGTCCTGCAAAGACAATCGCACCACAGAATGCTCCAAGAGATGAGTCTAGGGGC CGTTCCTCGTTTTATCCTGATGGTGGAGATCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCCTGCTTG ATAGGGGTAATACCAGGGATAAAGAGGCTTCAAAAGAGAAAGGATCAGAGAAAGGGAGGGCAGAGGGAGAATGGGAAGATCAGGAAGCTCTAGATTACTT CAGTGATAAAGAGTCTGGAAAACAAAAGTTTAATGATTCAGAAGGGGATGACACAGAGGAGACAGAGGATTATAGACAGTTCAGGAAGTCAGTCCTCGCA GATCAGGGTAAAAGTTTTGCTACTGCATCTCACCGGAATACTGAGGAGGAAGGACTCAAGTACAAGTCCAAAGTTTCACTGAAAGGCAATAGAGAAAGTG ATGGATTTAGAGAAGAAAAAAATTATAAACTTAAAGAGACTGGATATGTAGTGGAAAGGCCTAGCACTACAAAAGATAAGCACAAAGAAGAAGACAAAAA TTCTGAAAGAATAACAGTAAAGAAAGAAACTCAGTCACCTGAGCAGGTAAAGTCTGAAAAGCTCAAAGACCTCTTTGATTACAGTCCCCCTCTACACAAG AATCTGGATGCACGAGAAAAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAATGATAGCGAGTGATTCTCACCGTCCTGAAGTCAAACTCAAAA TGGCACCTGTTCCTCTTGATGATTCTAACAGACCTGCTTCCTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAGAAGGAGCAAGA ATTCCGATCCATCTTTGACCACATTAAGTTGCCACAGGCCAGCAAAAGCACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCATCATGTTAAA GAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAAAGAGCCCTGAAA TACACAGGAGAATTGACATCTCACCAAGTACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAAAAGGGAGATAA AAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTCCAGGGAATCC AGTGGATCAAGAAAGCAGGAAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGATCATTCTCGAT CTTCATCCTCTTCAGCATCACCTTCTTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCACCATGAAATGAA AGAATACTCAGGCTTTGCAGGAGTTAGCCGACCACGAGGAACCTTTCATGACGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAAGAGGTCGT GGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAATCAGGTAGCAGTCCTAAATGGACTCATGACAAATACCAAGGGGATGGGATTGTTGAAG ATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAGAAAAGGAATAATAAATATGAAGTAAGATTACAACAGAGCAGAACTT GCACCCACCATTTTTTTTACCTGATTTTTGTTTTCAAATAAGAATGTAAGCATTTTACTTAAATTTTACTGTTTGCAAGTAGTCTATAGAAATTTTGTTT TAAGTCTTCAAATATCTTGAGAAATAGTAGACTGTATGTTGAAAATTGTACTGAAATAAAGTAGAAAATTGTTACGTACCATATTTGTAACTATCAACTT TTAAAACTTTTAACGTTTTTGTTACATGCATTGTAATTCTGCTTTGTCTATAAGATATGGTCAAGTACAGCTCTGTGAAAGTTCTGATTCTCTTCCTTCC CTGTTTGTCAATGTTTTATTCTGAAGTAAACGTTAGCTCTACATATAAATCCTGGAACAGAAATTGTTTATAGAGACTACACTAATTATTTTAACTGTAT ACATCTGTTTAATTTGAACACACTACATCGTAGGGTGACTGATTTTTGAAGTATACCACAGACAAAAAGTTGTTACTATGGTAAACTAAGCTAGTTTAAC ACTTGAGCAAATGCTTAAGAAGGAATTAAAAAAAAAAAGCTTTGCCAATAGCTAAAAAGTACAAGCTATTAAAAATCAGATTGAAAAGTTTTGAGAAAAT GTTATTTTTACTGAAAGCAAGCAGTGGCCTATAAAGAACATTCTTAGGAGCCTTTTCTATTTGCGTTCAAAACTGTGTGTTCTCTTTCTATTCCTATTTG ATAGTTTGAGTCATGGTCTTAGATATTAGCTATTTGTGAGAGGAAACTGGTTTGTAACAATACTGCAAATAGAAACCCCATTTCTACTGAACATCCTAGT TTTAAACAGAAGAAAAACTGTAATCCTGGGGTTGGTATGTAGGAGGTCTATCCTGCAGAATAAGTTGATACATTAGTACCTGATTTCATATCTTACATAT TTATTTGAGCTGAACATTAGTTTGTAGTGTAACTATTAGTAAAAATAGAGAAACACAGCATACTGTTCATTAATAGTATTTTAAAAAAATTGTTTTTCAA ATGTCACCAATAAAAGTTTTGGCAGGAAGCTTGTTGCGGCATTGATCTAACCTTTTTCCCCCCCATTTCAGTTGCAGTTTTTGTAGAATGGCTTTTTCTT TTTCCTCTTAAGAGTTCTATTCTTCAGGTAGATAATTTTTCAAATGTGAATTATCTTTTGTGTCTATATTGATAGCTCTTAAAGGAGTGAAAATCTAAAA TAGTAAATTTCAATGTTAAGTGTCTGCTTTATGGGCATATATAAAAGTAGACACATTTCATTTGTTAATTTAGTTGTGTGTGTGTGTTAAAAGGAGCTAA TGCTTATTCTGTTAATGTAAACTTTTGAAGATCTTAAGTGTATTGCTCTTTCATCTTAAACACTTTCGAGGATTTGCAGTGCGTCTAGCACCTAGATTAC AGCCAGGAACATTGGTTAAGAACTGTTGGAAACAAAACTAAAAGCAAACTCAACATATGTGATGTTTATGGCCCTCAGATCCTTAGTATTGTGTGATTTT CCCCCGTTAACATGTCTTTCTAAAATTGTCTATTAAAGCAGAGGAAATACCTGCCAAAGGAAGTATGTATTGCATTAATCAGGGCATAACTAATATTCTC CTGTTCAGAATAATACTTATTTACGTGTGAAAGCAACATGGATGTGATTCCCAACACAGAATTTTCATGACCCTTTTATTGTATACAAATAAATACCATA ACAGTTACTTGGTTAGACATCAAATCTGTGTGCATGACTATGTGCTTATCCACTTAAGACAATAGGTAAAAGGGGATCTGAGAAATTATGTAATAGGGAG TGGGAATAAAACTACTTAATTCCTGTGGGCAGGTTATATTTTAAGTTCAAATGCATTGCTTTAACCTTTGGTTACTTTTATTCTGTTAAACAGAATTGAA GAAAGAGTATTATACCAGAGTGTAGTAGGCTAGGGTGATTGTAAGAACTCTGTAATAGAATGTCATTGTGGATGTTACCTTTTTCAGATCCAAGCATATA AAAAGCCTGTATATTTTTTAAAAACACATCTTAACTCCACGCTTTACGATATTATAAAAGTTGAATGGTTCCTCTTGGTAAGGATATTTGCTTACAAGTG CTAGGAAATAACTCACTGATACCTGCGTTAACATACTTTGTTTTGCCTAGAGAGGGGCAATAAAAATGAACCAAAGGATATTTCCAGAAAGGATTAAGAA AGCTGTTTAAGAAGGCCATGACTCTTTAGGTGTGTATGTGTACCTTTCAGCATCCTAGGAATTTTTATACTAAAAGCAAAATGTTTTTTCCAGTTAGTCT TCTTCAAGGAATTACTATTGTTCCTTTTGTCACAGGTAAAATCAGTGTTGGGAATTATAATTTGAGAAAAATATTACCCAGTAACATTGAATGTAGATGG CTAAACGATTCTTACTCAGTGTGATGTATAATGATGCAACAGGGACCCTTGTAAATTGTCATACGCCAATAAAATGTCACAAGTAATAACTGCTGTTGTT TGTTTACCTGTGTCTATTTCACACATCTTATTTCTGTGGCCTATTTTAGAATATCAGCGCATCTGTTAGGAAGATTACTGGTGTGGTAAGGCTTGATAAA TGCTTTATAAACTCCCTCAAACCTTTCTTTACTGTTTTTTTGTTTTGTCTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTCCCTTGT CTCCCCTGGGAAAATGGGAAATTTTACAGTTGGTAAATCTAAGCCAAAATTATTTTGAAATAAAGGAATTCTGGATGTCCAGTTTAGTCCTCGTTTTCTT ACGTTAATCTGGGACCTTATCACCCATAATATGGTGATTACTTCTCTTTCTAAAAACATAGTAGCTAGTAAATAAGTAAAAAGAATTGTCTTTTCATTCA CTTTAAGTAAGATGTGGTATAATTCTTACCATGTGCCATCCTGTCAGTTTTAACAAAGCATTTTCACAGAAATTTGTGTACTAAGACAAACTGACACATT TTGACTCATACAAATGGCAAATTAGTCCTTAAAAATTCTGTGAGAGAAATAACTCTGTGTGTACATACATATGCATGTAAAGTGTTGTGTAAGATCATTG GTAGCTTAATTATACTGGATAATTGTAATGTTATATACAAATTTCTTATATAAAAGTATGCTGCATTATTAACATTTGTTTAACTTTTCTTATTTTTCTT TTTCTGGGTTTCAACTGGAATCCCATTGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGACTTTCACATCCAGAAAGAGAAGTTCAGAGGCCTT TCTCAATTTACAGAGTTGAAGAGGAAAGGCAAATGGAGGAATAAACTTCATTGTCATTGGGATTAATTTGGCTAGACTCAATCTCTTGAGCCTTAAAATG AGAAAGTAATATCCATTACATGTTTAGAGTTCTCAAATAGTTGATACTATTATTAAATGTTTCAAATTTTATTGGGGTAGATACTAAAAATTAGTACACT GTCCTAATGAAGTTTAAATGAACAAGTTTTACATTTCTTAGGATTCAGGAATATATGGAATCTAGTTGTATTTAATTGACTTGAGAGCCACAGAGATTGT GTTGATTTATGAGCTGGACTCCTATTGTTTTAGAAACATGCAGTAATGATTAACTTGTGTCAATGATGGTAATGAGACCAAACTTGTACATTTAATTAAG GGTACCAGTTCTTTTTATGTTACTAGACTATACCTTGGCCTTTAAAAATGAATCTCACTGATTAAAGAGAACAGGCATTATTAGGATAGCATTCCACCAC AACTAGAAACATTCAAATAATGTGTCTTAATTGTAATCTGTATATAGGAAAATTTTTCCTATGGATATTTTTGGTGTTTTACCACAGTGAACTGATTTGT AGCACTTATGAAGTGCAGAAGGTAATATTCTTGAAAATAGAAAAAGGTTGGGTGAGCAGGCTTTAATGCCTTTCCCCCAAGAATATACATCGAATTTTTC TTAATCTTTTGGGGTTGGCCAGCTTCCAGATTTCATTAATAATGAGCTCTGCCTTTAATAAAAGTACATGATCATAGCTACACTGTATGTTTAGGTGGTG TGAAATGATTTATAATCACAGCTTGAACTGTGTTTGCTTGGTACTGTCATAGTGATTACAAATTTCATGGAATGCGAAGAGCAACAATAAATAAAAAATA CCACTCACCCTCCAGAA

NM_014739-3 (forward primer)
NM_014739-4 (reverse primer)
Single exon skipping

Frame 0

TPQIGSDGDVLARRR*LYGFPRVLESGNGRCVV ATEINSRNRDSACQEFEFRV*FLRAFSPGRIFTV SQRLHLTS*SCIKPRRKEMGRSNSRSHSSRSKS RSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRD RMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGG GGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKR RSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPY SKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQ EEPKDTFEHDPSESIDEFNKSSATSGDIWPGLS AYDNSPRSPHSPSPIATPPSQSSSCSDAPMLST VHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRY SPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRG RSSFYPDGGDQETAKTGKFLKRFTDEESRVFLL DRGNTRDKEASKEKGSEKGRAEGEWEDQEALDYF SDKESGKQKFNDSEGDDTEETEDYRQFRKSVLA DQGKSFATASHRNTEEEGLKYKSKVSLKGNRES DGFREEKNYKLKETGYVVERPSTTKDKHKEEDKN SERITVKKETQSPEQVKSEKLKDLFDYSPPLHK NLDAREKSTFREESPLRIKMIASDSHRPEVKLK MAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQE FRSIFDHIKLPQASKSTSESFIQHIVSLVHHVK EQYFKSAAMTLNERFTSYQKATEEHSTRQKSPE IHRRIDISPSTLRKHTRLAGEERVFKEENQKGDK KLRCDSADLRHDIDRRRKERSKERGDSKGSRES SGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSR SSSSSASPSSPSSREEKESKKEREEEFKTHHEMK EYSGFAGVSRPRGTFHDDRDDGVDYWAKRGRGR GTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVE DEEETMENNEEKKDRRKEEKE**I*SKITTEQNL HPPFFLPDFCFQIRM*AFYLNFTVCK*SIEILF *VFKYLEK**TVC*KLY*NKVENCYVPYL*LST FKTFNVFVTCIVILLCL*DMVKYSSVKVLILFLP CLSMFYSEVNVSSTYKSWNRNCL*RLH*LF*LY TSV*FEHTTS*GD*FLKYTTDKKLLLW*TKLV* HLSKCLRRN*KKKALPIAKKYKLLKIRLKSFEKM LFLLKASSGL*RTFLGAFSICVQNCVFSFYSYL IV*VMVLDISYL*EETGL*QYCK*KPHFY*TS* F*TEEKL*SWGWYVGGLSCRIS*YIST*FHILHI YLS*TLVCSVTISKNRETQHTVH**YFKKIVFQ MSPIKVLAGSLLRH*SNLFPPHFSCSFCRMAFS FSS*EFYSSGR*FFKCELSFVSILIALKGVKI*N SKFQC*VSALWAYIKVDTFHLLI*LCVCVKRS* CLFC*CKLLKILSVLLFHLKHFRGFAVRLAPRL QPGTLVKNCWKQN*KQTQHM*CLWPSDP*YCVIF PR*HVFLKLSIKAEEIPAKGSMYCINQGITNIL LFRIILIYV*KQHGCDSQHRIFMTLLLYTNKYH NSYLVRHQICVHDYVLIHLRQ*VKGDLRNYVIGS GNKTT*FLWAGYILSSNALL*PLVTFILLNRIE ERVLYQSVVG*GDCKNSVIECHCGCYLFQIQAY KKPVYFLKTHLNSTLYDIIKVEWFLLVRIFAYKC *EITH*YLR*HTLFCLERGNKNEPKDISRKD*E SCLRRP*LFRCVCVPFSILGIFILKAKCFFQLV FFKELLLFLLSQVKSVLGIII*EKYYPVTLNVDG *TILTQCDV**CNRDPCKLSYANKMSQVITAVV CLPVSISHILFLWPILEYQRIC*EDYWCGKA** MLYKLPQTFLYCFFVLSCFCFCFCFCFCFCFSLV SPGKMGNFTVGKSKPKLF*NKGILDVQFSPRFL TLIWDLITHNMVITSLSKNIVASK*VKRIVFSF TLSKMWYNSYHVPSCQF*QSIFTEICVLRQTDTF *LIQMAN*SLKIL*EK*LCVYIHMHVKCCVRSL VA*LYWIIVMLYTNFLYKSMLHY*HLFNFSYFS FSGFQLESHWWGCTLSFLVE**TFTSRKRSSEAF LNLQS*RGKANGGINFIVIGINLARLNLLSLKM RK*YPLHV*SSQIVDTIIKCFKFYWGRY*KLVH CPNEV*MNKFYIS*DSGIYGI*LYLIDLRATEIV LIYELDSYCFRNMQ**LTCVNDGNETKLVHLIK GTSSFYVTRLYLGL*K*ISLIKENRHY*DSIPP QLETFK*CVLIVICI*ENFSYGYFWCFTTVN*FV ALMKCRR*YS*K*KKVG*AGFNAFPPRIYIEFF LIFWGWPASRFH***ALPLIKVHDHSYTVCLGG VK*FIITA*TVFAWYCHSDYKFHGMRRATINKKY HSPSR

Frame 1

RPRSEVTETC*RVEGSSMVFLAFLSREMAAVWL QRR*IPGTAIRRVRNSNLEFNFSEHSLQEEFLQ YLKDFT*LLDPA*NQGEKKWVAPILDHILQGQSL DHSLVLDQDQDLILERSDTGLVPEHIQGLVVEI VCILEIIVAITEIIEE*DDLMGTEEGVEGIIKE EEVDIIEVVIDLSGIEGTLGVLDEVVHVPGVQKE DPFLLKDPEADLAGHIDLLGLQDHPLLVLHPHI ANLLFLKDEGLRKNKPKKLKGNPKKRVR*KVNH RRNRKIHLNMTHLSLSMNLISHQPHPVIFGLAFQ LMIIVLDHPIVLHLLLHHLVRVHLALMLPCSVQ FTLQKILLLSIHIPFSIVLKGLGLVLLEMDLVD TVLLRIVQFITSLHEEVLQRQSHHRMLQEMSLGA VPRFILMVEIRKLQRLGSS*KGSQMKSLEYSCL IGVIPGIKRLQKRKDQRKGGQRENGKIRKL*IT SVIKSLENKSLMIQKGMTQRRQRIIDSSGSQSSQ IRVKVLLLHLTGILRRKDSSTSPKFH*KAIEKV MDLEKKKIINLKRLDM*WKGLALQKISTKKKTK ILKE*Q*RKKLSHLSR*SLKSSKTSLITVPLYTR IWMHEKSLPSERKAHLGSK**RVILTVLKSNSK WHLFLLMILTDLLP*LKTGCLLVHLSILSRRSK NSDPSLTTLSCHRPAKALQSHLFNTLCPWFIMLK SNTSSQLQ*P*TSGSLRIRKPLKNIVLGKRALK YTGELTSHQVP*GSIPV*QGKREFLKKKIKREI KN*GVTLLTFGMTLIAVEKKEVKNGEIPRAPGNP VDQESRKKLQKITRNTNLTKMTVNIKESKIILD LHPLQHHLLLPVLEKKRRVRRKEKKNLKLTMK* KNTQALQELADHEEPFMTTEMMVWIIGPKEEEVV VLFNVAEGALTSKNQVAVLNGLMTNTKGMGLLK MKKRPWKIMKKRRTDARKKRNNKYEVRLQQSRT CTHHFFYLIFVFK*ECKHFT*ILLFASSL*KFCF KSSNILRNSRLYVENCTEIK*KIVTYHICNYQL LKLLTFLLHAL*FCFVYKIWSSTAL*KF*FSSF PVCQCFILK*TLALHINPGTEIVYRDYTNYFNCI HLFNLNTLHRRVTDF*SIPQTKSCYYGKLS*FN T*ANA*EGIKKKKLCQ*LKSTSY*KSD*KVLRK CYFY*KQAVAYKEHS*EPFLFAFKTVCSLSIPI* *FESWS*ILAICERKLVCNNTANRNPISTEHPS FKQKKNCNPGVGM*EVYPAE*VDTLVPDFISYI FI*AEH*FVV*LLVKIEKHSILFINSILKKLFFK CHQ*KFWQEACCGIDLTFFPPISVAVFVEWLFL FPLKSSILQVDNFSNVNYLLCLY**LLKE*KSK IVNFNVKCLLYGHI*K*THFIC*FSCVCVLKGAN AYSVNVNF*RS*VYCSFILNTFEDLQCV*HLDY SQEHWLRTVGNKTKSKLNICDVYGPQILSIV*F SPVNMSF*NCLLKQRKYLPKEVCIALIRA*LIFS CSE*YLFTCESNMDVIPNTEFS*PFYCIQINTI TVTWLDIKSVCMTMCLST*DNR*KGI*EIM**G VGIKLLNSCGQVIF*VQMHCFNLWLLLFC*TELK KEYYTRV**ARVIVRTL**NVIVDVTFFRSKHI KSLYIF*KHILTPRFTIL*KLNGSSW*GYLLTS ARK*LTDTCVNILCFA*RGAIKMNQRIFPERIKK AV*EGHDSLGVYVYLSAS*EFLY*KQNVFSS*S SSRNYYCSFCHR*NQCWEL*FEKNITQ*H*M*M AKRFLLSVMYNDATGTLVNCHTPIKCHK**LLLF VYLCLFHTSYFCGLF*NISASVRKITGVVRLDK CFINSLKPFFTVFLFCLVFVFVFVFVFVFVFPL SPLGKWEILQLVNLSQNYFEIKEFWMSSLVLVFL R*SGTLSPIIW*LLLFLKT**LVNK*KELSFHS L*VRCGIILTMCHPVSFNKAFSQKFVY*DKLTH FDSYKWQISP*KFCERNNSVCTYICM*SVV*DHW *LNYTG*L*CYIQISYIKVCCIINICLTFLIFL FLGFNWNPIGGDVP*VSLWNNRLSHPEREVQRP FSIYRVEEERQMEE*TSLSLGLIWLDSIS*ALK* ESNIHYMFRVLK*LILLLNVSNFIGVDTKN*YT VLMKFK*TSFTFLRIQEYMESSCI*LT*EPQRL C*FMSWTPIVLETCSND*LVSMMVMRPNLYI*LR VPVLFMLLDYTLAFKNESH*LKRTGIIRIAFHH N*KHSNNVS*L*SVYRKIFPMDIFGVLPQ*TDL *HL*SAEGNILENRKRLGEQALMPFPQEYTSNFS *SFGVGQLPDFINNELCL**KYMIIATLYV*VV *NDL*SQLELCLLGTVIVITNFMECEEQQ*IKN TTHPPE

Frame 2

APDRK*RRRASASKVALWFSSRS*VGKWPLCGC NGDKFPEPRFGVSGIRI*SLISQSILSRKNFYS ISKTSLDFLILHKTKEKRNGSLQF*ITFFKVKV *ITV*FSIKIKISF*KEAIQVSFQNIFKVS**RS YVF*RLSSRLQK**RNETTLWVQRKG*RVLSRR RR*ISSRWL*TCLE*KAL*ES*TRSFTFQESKK KIRFFSKIQKQISPVI*IF*VSKIILFSFFIPI* QISCF*KTRVSGKTNQKS*RGTPRRESVEK*IT GGTERYI*T*PI*VYR*I**VISHIR*YLAWPF SL***S*ITP*SFTYCYTT*SEFILL*CSHAQYS SLCKKYSFSAFTFHSA*S*KVWVWFCWKWI*SI QSFSE*SNSSHPFTKKSCKDNRTTECSKR*V*G PFLVLS*WWRSGNCKDWEVLKKVHR*RV*SIPA* *G*YQG*RGFKRERIREREGRGRMGRSGSSRLL Q**RVWKTKV**FRRG*HRGDRGL*TVQEVSPR RSG*KFCYCISPEY*GGRTQVQVQSFTERQ*RK* WI*RRKKL*T*RDWICSGKA*HYKR*AQRRRQK F*KNNSKERNSVT*AGKV*KAQRPL*LQSPSTQ ESGCTRKVYLQRGKPT*DQNDSE*FSPS*SQTQN GTCSS**F*QTCFLD*RQAAC*YTCPFCQEGAR IPIHL*PH*VATGQQKHFRVIYSTHCVLGSSC* RAILQVSCNDPKRAVHFVSESH*RT*YSAKEP*N TQEN*HLTKYPEEAYPFSRGRESF*RRKSKGR* KIKV*LC*PSA*H*SP*KRKK*RTGRFQGLQGI QWIKKAGKNSKRLQGIQILQR*Q*T*KRARSFSI FILFSITFFSQFSRRKGE*EGKRRRI*NSP*NE RILRLCRS*PTTRNLS*RQR*WCGLLGQKRKRS WYFSTWQRAL*LQKIR*QS*MDS*QIPRGWDC*R *RRDHGK**RKEGQTQGRKGIINMK*DYNRAEL APTIFFT*FLFSNKNVSILLKFYCLQVVYRNFV LSLQIS*EIVDCMLKIVLK*SRKLLRTIFVTINF *NF*RFCYMHCNSALSIRYGQVQLCESSDSLPS LFVNVLF*SKR*LYI*ILEQKLFIETTLIILTV YICLI*THYIVG*LIFEVYHRQKVVTMVN*ASLT LEQMLKKELKKKSFANS*KVQAIKNQIEKF*EN VIFTESKQWPIKNILRSLFYLRSKLCVLFLFLF DSLSHGLRY*LFVRGNWFVTILQIETPFLLNILV LNRRKTVILGLVCRRSILQNKLIH*YLISYLTY LFELNISL*CNY**K*RNTAYCSLIVF*KNCFS NVTNKSFGRKLVAALI*PFSPPFQLQFL*NGFFF FLLRVLFFR*IIFQM*IIFCVYIDSS*RSENLK **ISMLSVCFMGIYKSRHISFVNLVVCVC*KEL MLILLM*TFEDLKCIALSS*TLSRICSASST*IT ARNIG*ELLETKLKANSTYVMFMALRSLVLCDF PPLTCLSKIVY*SRGNTCQRKYVLH*SGHN*YS PVQNNTYLRVKATWM*FPTQNFHDPFIVYK*IP* QLLG*TSNLCA*LCAYPLKTIGKRGSEKLCNRE WE*NYLIPVGRLYFKFKCIALTFGYFYSVKQN* RKSIIPECSRLG*L*ELCNRMSLWMLPFSDPSI* KACIFFKNTS*LHALRYYKS*MVPLGKDICLQV LGNNSLIPALTYFVLPREGQ*K*TKGYFQKGLR KLFKKAMTL*VCMCTFQHPRNFYTKSKMFFPVSL LQGITIVPFVTGKISVGNYNLRKILPSNIECRW LNDSYSV*CIMMQQGPL*IVIRQ*NVTSNNCCC LFTCVYFTHLISVAYFRISAHLLGRLLVW*GLIN AL*TPSNLSLLFFCFVLFLFLFLFLFLFLFFPC LPWENGKFYSW*I*AKIILK*RNSGCPV*SSFS YVNLGPYHP*YGDYFSF*KHSS**ISKKNCLFIH FK*DVV*FLPCAILSVLTKHFHRNLCTKTN*HI LTHTNGKLVLKNSVREITLCVHTYACKVLCKII GSLIILDNCNVIYKFLI*KYAALLTFV*LFLFFF FWVSTGIPLVGMYLKFPCGIIDFHIQKEKFRGL SQFTELKRKGKWRNKLHCHWD*FG*TQSLEP*N EKVISITCLEFSNS*YYY*MFQILLG*ILKISTL S**SLNEQVLHFLGFRNIWNLVVFN*LESHRDC VDL*AGLLLF*KHAVMINLCQ*W**DQTCTFN* GYQFFLCY*TIPWPLKMNLTD*REQALLG*HSTT TRNIQIMCLNCNLYIGKFFLWIFLVFYHSELIC STYEVQKVIFLKIEKGWVSRL*CLSPKNIHRIF LNLLGLASFQISLIMSSAFNKST*S*LHCMFRWC EMIYNHSLNCVCLVLS**LQISWNAKSNNK*KI PLTLQ

Sequence BCLAF1.fAug05

CATCATGTTAAAGAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAA AGAGCCCTGAAATACACAGGAGAATTGACATCTCACCAAGTACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCA AAAGGGAGATAAAAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGC TCCAGGGAATCCAGTGGATCAAGAAAGCAGGAAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGCAAAAATGTATTTGTCTTTT ACACGGCTTATGGTGGATGAGGACAAAAACTTTGGAATAGCAAGCTAACACTGAAAAAAAAAATGACAAATTAATGTGTCCTATTATCCAGGCTACATCA GGAGGTTGGACTTCAATGCAGTGCATTTCTAGAAACAGTTTGTTAAACATAAAAGAGAGCAAGATCATTCTCGATCTTCATCCTCTTCAGCATCACCTTC TTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCACCATGAAATGAAAGAATACTCAGGCTTTGCAGGAGTT AGCCGACCACGAGGAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAACAACTCAAATACTACTT TTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTCTTGCATGACGACAGAGATGATGGTGTGGATTATTG GGCCAAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAATCAGGTAGCAGTCCTAAATGGACTCATGACAAATAC CAAGGGGATGGGATTGTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAGAAAAGGAATAATAAATATGAAGTA AGATTACAACAGAGCAGAACTTGCACCCACCATTTTTTTTACCTGATTTTTGTTTTCAAATAAGAATGTAAGCATTTTACTTAAATTTTACTGTTTGCAA GTAGTCTATAGAAATTTTGTTTTAAGTCTTCAAATATCTTGAGAAATAGTAGACTGTATGTTGAAAATTGTACTGAAATAAAGTAGAAAATTGTTACGTA CCATATTTGTAACTATCAACTTTTAAAACTTTTAACGTTTTTGTTACATGCATTGTAATTCTGCTTTGTCTATAAGATATGGTCAAGTACAGCTCTGTGA AAGTTCTGATTCTCTTCCTTCCCTGTTTGTCAATGTTTTATTCTGAAGTAAACGTTAGCTCTACATATAAATCCTGGAACAGAAATTGTTTATAGAGACT ACACTAATTATTTTAACTGTATACATCTGTTTAATTTGAACACACTACATCGTAGGGTGACTGATTTTTGAAGTATACCACAGACAAAAAGTTGTTACTA TGGTAAACTAAGCTAGTTTAACACTTGAGCAAATGCTTAAGAAGGAATTAAAAAAAAAAAGCTTTGCCAATAGCTAAAAAGTACAAGCTATTAAAAATCA GATTGAAAAGTTTTGAGAAAATGTTATTTTTACTGAAAGCAAGCAGTGGCCTATAAAGAACATTCTTAGGAGCCTTTTCTATTTGCGTTCAAAACTGTGT GTTCTCTTTCTATTCCTATTTGATAGTTTGAGTCATGGTCTTAGATATTAGCTATTTGTGAGAGGAAACTGGTTTGTAACAATACTGCAAATAGAAACCC CATTTCTACTGAACATCCTAGTTTTAAACAGAAGAAAAACTGTAATCCTGGGGTTGGTATGTAGGAGGTCTATCCTGCAGAATAAGTTGATACATTAGTA CCTGATTTCATATCTTACATATTTATTTGAGCTGAACATTAGTTTGTAGTGTAACTATTAGTAAAAATAGAGAAACACAGCATACTGTTCATTAATAGTA TTTTAAAAAAATTGTTTTTCAAATGTCACCAATAAAAGTTTTGGCAGGAAGCTTGTTGCGGCATTGATCTAACCTTTTTCCCCCCCATTTCAGTTGCAGT TTTTGTAGAATGGCTTTTTCTTTTTCCTCTTAAGAGTTCTATTCTTCAGGTAGATAATTTTTCAAATGTGAATTATCTTTTGTGTCTATATTGATAGCTC TTAAAGGAGTGAAAATCTAAAATAGTAAATTTCAATGTTAAGTGTCTGCTTTATGGGCATATATAAAAGTAGACACATTTCATTTGTTAATTTAGTTGTG TGTGTGTGTTAAAAGGAGCTAATGCTTATTCTGTTAATGTAAACTTTTGAAGATCTTAAGTGTATTGCTCTTTCATCTTAAACACTTTCGAGGATTTGCA GTGCGTCTAGCACCTAGATTACAGCCAGGAACATTGGTTAAGAACTGTTGGAAACAAAACTAAAAGCAAACTCAACATATGTGATGTTTATGGCCCTCAG ATCCTTAGTATTGTGTGATTTTCCCCCGTTAACATGTCTTTCTAAAATTGTCTATTAAAGCAGAGGAAATACCTGCCAAAGGAAGTATGTATTGCATTAA TCAGGGCATAACTAATATTCTCCTGTTCAGAATAATACTTATTTACGTGTGAAAGCAACATGGATGTGATTCCCAACACAGAATTTTCATGACCCTTTTA TTGTATACAAATAAATACCATAACAGTTACTTGGTTAGACATCAAATCTGTGTGCATGACTATGTGCTTATCCACTTAAGACAATAGGTAAAAGGGGATC TGAGAAATTATGTAATAGGGAGTGGGAATAAAACTACTTAATTCCTGTGGGCAGGTTATATTTTAAGTTCAAATGCATTGCTTTAACCTTTGGTTACTTT TATTCTGTTAAACAGAATTGAAGAAAGAGTATTATACCAGAGTGTAGTAGGCTAGGGTGATTGTAAGAACTCTGTAATAGAATGTCATTGTGGATGTTAC CTTTTTCAGATCCAAGCATATAAAAAGCCTGTATATTTTTTAAAAACACATCTTAACTCCACGCTTTACGATATTATAAAAGTTGAATGGTTCCTCTTGG TAAGGATATTTGCTTACAAGTGCTAGGAAATAACTCACTGATACCTGCGTTAACATACTTTGTTTTGCCTAGAGAGGGGCAATAAAAATGAACCAAAGGA TATTTCCAGAAAGGATTAAGAAAGCTGTTTAAGAAGGCCATGACTCTTTAGGTGTGTATGTGTACCTTTCAGCATCCTAGGAATTTTTATACTAAAAGCA AAATGTTTTTTCCAGTTAGTCTTCTTCAAGGAATTACTATTGTTCCTTTTGTCACAGGTAAAATCAGTGTTGGGAATTATAATTTGAGAAAAATATTACC CAGTAACATTGAATGTAGATGGCTAAACGATTCTTACTCAGTGTGATGTATAATGATGCAACAGGGACCCTTGTAAATTGTCATACGCCAATAAAATGTC ACAAGTAATAACTGCTGTTGTTTGTTTACCTGTGTCTATTTCACACATCTTATTTCTGTGGCCTATTTTAGAATATCAGCGCATCTGTTAGGAAGATTAC TGGTGTGGTAAGGCTTGATAAATGCTTTATAAACTCCCTCAAACCTTTCTTTACTGTTTTTTTGTTTTGTCTTGTTTTTGTTTTTGTTTTTGTTTTTGTT TTTGTTTTTGTTTTTCCCTTGTCTCCCCTGGGAAAATGGGAAATTTTACAGTTGGTAAATCTAAGCCAAAATTATTTTGAAATAAAGGAATTCTGGATGT CCAGTTTAGTCCTCGTTTTCTTACGTTAATCTGGGACCTTATCACCCATAATATGGTGATTACTTCTCTTTCTAAAAACATAGTAGCTAGTAAATAAGTA AAAAGAATTGTCTTTTCATTCACTTTAAGTAAGATGTGGTATAATTCTTACCATGTGCCATCCTGTCAGTTTTAACAAAGCATTTTCACAGAAATTTGTG TACTAAGACAAACTGACACATTTTGACTCATACAAATGGCAAATTAGTCCTTAAAAATTCTGTGAGAGAAATAACTCTGTGTGTACATACATATGCATGT AAAGTGTTGTGTAAGATCATTGGTAGCTTAATTATACTGGATAATTGTAATGTTATATACAAATTTCTTATATAAAAGTATGCTGCATTATTAACATTTG TTTAACTTTTCTTATTTTTCTTTTTCTGGGTTTCAACTGGAATCCCATTGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGACTTTCACATCCA GAAAGAGAAGTTCAGAGGCCTTTCTCAATTTACAGAGTTGAAGAGGAAAGGCAAATGGAGGAATAAACTTCATTGTCATTGGGATTAATTTGGCTAGACT CAATCTCTTGAGCCTTAAAATGAGAAAGTAATATCCATTACATGTTTAGAGTTCTCAAATAGTTGATACTATTATTAAATGTTTCAAATTTTATTGGGGT AGATACTAAAAATTAGTACACTGTCCTAATGAAGTTTAAATGAACAAGTTTTACATTTCTTAGGATTCAGGAATATATGGAATCTAGTTGTATTTAATTG ACTTGAGAGCCACAGAGATTGTGTTGATTTATGAGCTGGACTCCTATTGTTTTAGAAACATGCAGTAATGATTAACTTGTGTCAATGATGGTAATGAGAC CAAACTTGTACATTTAATTAAGGGTACCAGTTCTTTTTATGTTACTAGACTATACCTTGGCCTTTAAAAATGAATCTCACTGATTAAAGAGAACAGGCAT TATTAGGATAGCATTCCACCACAACTAGAAACATTCAAATAATGTGTCTTAATTGTAATCTGTATATAGGAAAATTTTTCCTATGGATATTTTTGGTGTT TTACCACAGTGAACTGATTTGTAGCACTTATGAAGTGCAGAAGGTAATATTCTTGAAAATAGAAAAAGGTTGGGTGAGCAGGCTTTAATGCCTTTCCCCC AAGAATATACATCGAATTTTTCTTAATCTTTTGGGGTTGGCCAGCTTCCAGATTTCATTAATAATGAGCTCTGCCTTTAATAAAAGTACATGATCATAGC TACACTGTATGTTTAGGTGGTGTGAAATGATTTATAATCACAGCTTGAACTGTGTTTGCTTGGTACTGTCATAGTGATTACAAATTTCATGGAATGCGAA GAGCAACAATAAATAAAAAATACCACTCACCCTCCAGAA

NM_014739-3 (forward primer)
NM_014739-4 (reverse primer)
Single exon skipping

Frame 0

HHVKEQYFKSAAMTLNERFTSYQKATEEHSTRQ KSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQ KGDKKLRCDSADLRHDIDRRRKERSKERGDSKG SRESSGSRKQEKTPKDYKEYKSYKDDSKNVFVF YTAYGG*GQKLWNSKLTLKKKMTN*CVLLSRLHQ EVGLQCSAFLETVC*T*KRARSFSIFILFSITF FSQFSRRKGE*EGKRRRI*NSP*NERILRLCRS *PTTRNLFSN*RQRKSQRSFCWDKYWSKQLKYYF SKETEGRGMGSRIYPKEQEVLLA*RQR*WCGLL GQKRKRSWYFSTWQRAL*LQKIR*QS*MDS*QI PRGWDC*R*RRDHGK**RKEGQTQGRKGIINMK* DYNRAELAPTIFFT*FLFSNKNVSILLKFYCLQ VVYRNFVLSLQIS*EIVDCMLKIVLK*SRKLLR TIFVTINF*NF*RFCYMHCNSALSIRYGQVQLCE SSDSLPSLFVNVLF*SKR*LYI*ILEQKLFIET TLIILTVYICLI*THYIVG*LIFEVYHRQKVVT MVN*ASLTLEQMLKKELKKKSFANS*KVQAIKNQ IEKF*ENVIFTESKQWPIKNILRSLFYLRSKLC VLFLFLFDSLSHGLRY*LFVRGNWFVTILQIET PFLLNILVLNRRKTVILGLVCRRSILQNKLIH*Y LISYLTYLFELNISL*CNY**K*RNTAYCSLIV F*KNCFSNVTNKSFGRKLVAALI*PFSPPFQLQ FL*NGFFFFLLRVLFFR*IIFQM*IIFCVYIDSS *RSENLK**ISMLSVCFMGIYKSRHISFVNLVV CVC*KELMLILLM*TFEDLKCIALSS*TLSRIC SASST*ITARNIG*ELLETKLKANSTYVMFMALR SLVLCDFPPLTCLSKIVY*SRGNTCQRKYVLH* SGHN*YSPVQNNTYLRVKATWM*FPTQNFHDPF IVYK*IP*QLLG*TSNLCA*LCAYPLKTIGKRGS EKLCNREWE*NYLIPVGRLYFKFKCIALTFGYF YSVKQN*RKSIIPECSRLG*L*ELCNRMSLWML PFSDPSI*KACIFFKNTS*LHALRYYKS*MVPLG KDICLQVLGNNSLIPALTYFVLPREGQ*K*TKG YFQKGLRKLFKKAMTL*VCMCTFQHPRNFYTKS KMFFPVSLLQGITIVPFVTGKISVGNYNLRKILP SNIECRWLNDSYSV*CIMMQQGPL*IVIRQ*NV TSNNCCCLFTCVYFTHLISVAYFRISAHLLGRL LVW*GLINAL*TPSNLSLLFFCFVLFLFLFLFLF LFLFFPCLPWENGKFYSW*I*AKIILK*RNSGC PV*SSFSYVNLGPYHP*YGDYFSF*KHSS**IS KKNCLFIHFK*DVV*FLPCAILSVLTKHFHRNLC TKTN*HILTHTNGKLVLKNSVREITLCVHTYAC KVLCKIIGSLIILDNCNVIYKFLI*KYAALLTF V*LFLFFFFWVSTGIPLVGMYLKFPCGIIDFHIQ KEKFRGLSQFTELKRKGKWRNKLHCHWD*FG*T QSLEP*NEKVISITCLEFSNS*YYY*MFQILLG *ILKISTLS**SLNEQVLHFLGFRNIWNLVVFN* LESHRDCVDL*AGLLLF*KHAVMINLCQ*W**D QTCTFN*GYQFFLCY*TIPWPLKMNLTD*REQA LLG*HSTTTRNIQIMCLNCNLYIGKFFLWIFLVF YHSELICSTYEVQKVIFLKIEKGWVSRL*CLSP KNIHRIFLNLLGLASFQISLIMSSAFNKST*S* LHCMFRWCEMIYNHSLNCVCLVLS**LQISWNAK SNNK*KIPLTLQ

Frame 1

IMLKSNTSSQLQ*P*TSGSLRIRKPLKNIVLGK RALKYTGELTSHQVP*GSIPV*QGKREFLKKKI KREIKN*GVTLLTFGMTLIAVEKKEVKNGEIPRA PGNPVDQESRKKLQKITRNTNLTKMTAKMYLSF TRLMVDEDKNFGIAS*H*KKK*QINVSYYPGYI RRLDFNAVHF*KQFVKHKREQDHSRSSSSSASPS SPSSREEKESKKEREEEFKTHHEMKEYSGFAGV SRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTT FQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYW AKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKY QGDGIVEDEEETMENNEEKKDRRKEEKE**I*S KITTEQNLHPPFFLPDFCFQIRM*AFYLNFTVCK *SIEILF*VFKYLEK**TVC*KLY*NKVENCYV PYL*LSTFKTFNVFVTCIVILLCL*DMVKYSSV KVLILFLPCLSMFYSEVNVSSTYKSWNRNCL*RL H*LF*LYTSV*FEHTTS*GD*FLKYTTDKKLLL W*TKLV*HLSKCLRRN*KKKALPIAKKYKLLKI RLKSFEKMLFLLKASSGL*RTFLGAFSICVQNCV FSFYSYLIV*VMVLDISYL*EETGL*QYCK*KP HFY*TS*F*TEEKL*SWGWYVGGLSCRIS*YIS T*FHILHIYLS*TLVCSVTISKNRETQHTVH**Y FKKIVFQMSPIKVLAGSLLRH*SNLFPPHFSCS FCRMAFSFSS*EFYSSGR*FFKCELSFVSILIA LKGVKI*NSKFQC*VSALWAYIKVDTFHLLI*LC VCVKRS*CLFC*CKLLKILSVLLFHLKHFRGFA VRLAPRLQPGTLVKNCWKQN*KQTQHM*CLWPS DP*YCVIFPR*HVFLKLSIKAEEIPAKGSMYCIN QGITNILLFRIILIYV*KQHGCDSQHRIFMTLL LYTNKYHNSYLVRHQICVHDYVLIHLRQ*VKGD LRNYVIGSGNKTT*FLWAGYILSSNALL*PLVTF ILLNRIEERVLYQSVVG*GDCKNSVIECHCGCY LFQIQAYKKPVYFLKTHLNSTLYDIIKVEWFLL VRIFAYKC*EITH*YLR*HTLFCLERGNKNEPKD ISRKD*ESCLRRP*LFRCVCVPFSILGIFILKA KCFFQLVFFKELLLFLLSQVKSVLGIII*EKYY PVTLNVDG*TILTQCDV**CNRDPCKLSYANKMS QVITAVVCLPVSISHILFLWPILEYQRIC*EDY WCGKA**MLYKLPQTFLYCFFVLSCFCFCFCFC FCFCFSLVSPGKMGNFTVGKSKPKLF*NKGILDV QFSPRFLTLIWDLITHNMVITSLSKNIVASK*V KRIVFSFTLSKMWYNSYHVPSCQF*QSIFTEIC VLRQTDTF*LIQMAN*SLKIL*EK*LCVYIHMHV KCCVRSLVA*LYWIIVMLYTNFLYKSMLHY*HL FNFSYFSFSGFQLESHWWGCTLSFLVE**TFTS RKRSSEAFLNLQS*RGKANGGINFIVIGINLARL NLLSLKMRK*YPLHV*SSQIVDTIIKCFKFYWG RY*KLVHCPNEV*MNKFYIS*DSGIYGI*LYLI DLRATEIVLIYELDSYCFRNMQ**LTCVNDGNET KLVHLIKGTSSFYVTRLYLGL*K*ISLIKENRH Y*DSIPPQLETFK*CVLIVICI*ENFSYGYFWC FTTVN*FVALMKCRR*YS*K*KKVG*AGFNAFPP RIYIEFFLIFWGWPASRFH***ALPLIKVHDHS YTVCLGGVK*FIITA*TVFAWYCHSDYKFHGMR RATINKKYHSPSR

Frame 2

SC*RAILQVSCNDPKRAVHFVSESH*RT*YSAK EP*NTQEN*HLTKYPEEAYPFSRGRESF*RRKS KGR*KIKV*LC*PSA*H*SP*KRKK*RTGRFQG LQGIQWIKKAGKNSKRLQGIQILQR*QQKCICLL HGLWWMRTKTLE*QANTEKKNDKLMCPIIQATS GGWTSMQCISRNSLLNIKESKIILDLHPLQHHL LLPVLEKKRRVRRKEKKNLKLTMK*KNTQALQEL ADHEEPFFELEAEEEPEEFLLGQILVQTTQILL FKRDRRKRNGIQNIPQRARSTSCMTTEMMVWII GPKEEEVVVLFNVAEGALTSKNQVAVLNGLMTNT KGMGLLKMKKRPWKIMKKRRTDARKKRNNKYEV RLQQSRTCTHHFFYLIFVFK*ECKHFT*ILLFA SSL*KFCFKSSNILRNSRLYVENCTEIK*KIVTY HICNYQLLKLLTFLLHAL*FCFVYKIWSSTAL* KF*FSSFPVCQCFILK*TLALHINPGTEIVYRD YTNYFNCIHLFNLNTLHRRVTDF*SIPQTKSCYY GKLS*FNT*ANA*EGIKKKKLCQ*LKSTSY*KS D*KVLRKCYFY*KQAVAYKEHS*EPFLFAFKTV CSLSIPI**FESWS*ILAICERKLVCNNTANRNP ISTEHPSFKQKKNCNPGVGM*EVYPAE*VDTLV PDFISYIFI*AEH*FVV*LLVKIEKHSILFINS ILKKLFFKCHQ*KFWQEACCGIDLTFFPPISVAV FVEWLFLFPLKSSILQVDNFSNVNYLLCLY**L LKE*KSKIVNFNVKCLLYGHI*K*THFIC*FSC VCVLKGANAYSVNVNF*RS*VYCSFILNTFEDLQ CV*HLDYSQEHWLRTVGNKTKSKLNICDVYGPQ ILSIV*FSPVNMSF*NCLLKQRKYLPKEVCIAL IRA*LIFSCSE*YLFTCESNMDVIPNTEFS*PFY CIQINTITVTWLDIKSVCMTMCLST*DNR*KGI *EIM**GVGIKLLNSCGQVIF*VQMHCFNLWLL LFC*TELKKEYYTRV**ARVIVRTL**NVIVDVT FFRSKHIKSLYIF*KHILTPRFTIL*KLNGSSW *GYLLTSARK*LTDTCVNILCFA*RGAIKMNQR IFPERIKKAV*EGHDSLGVYVYLSAS*EFLY*KQ NVFSS*SSSRNYYCSFCHR*NQCWEL*FEKNIT Q*H*M*MAKRFLLSVMYNDATGTLVNCHTPIKC HK**LLLFVYLCLFHTSYFCGLF*NISASVRKIT GVVRLDKCFINSLKPFFTVFLFCLVFVFVFVFV FVFVFPLSPLGKWEILQLVNLSQNYFEIKEFWM SSLVLVFLR*SGTLSPIIW*LLLFLKT**LVNK* KELSFHSL*VRCGIILTMCHPVSFNKAFSQKFV Y*DKLTHFDSYKWQISP*KFCERNNSVCTYICM *SVV*DHW*LNYTG*L*CYIQISYIKVCCIINIC LTFLIFLFLGFNWNPIGGDVP*VSLWNNRLSHP EREVQRPFSIYRVEEERQMEE*TSLSLGLIWLD SIS*ALK*ESNIHYMFRVLK*LILLLNVSNFIGV DTKN*YTVLMKFK*TSFTFLRIQEYMESSCI*L T*EPQRLC*FMSWTPIVLETCSND*LVSMMVMR PNLYI*LRVPVLFMLLDYTLAFKNESH*LKRTGI IRIAFHHN*KHSNNVS*L*SVYRKIFPMDIFGV LPQ*TDL*HL*SAEGNILENRKRLGEQALMPFP QEYTSNFS*SFGVGQLPDFINNELCL**KYMIIA TLYV*VV*NDL*SQLELCLLGTVIVITNFMECE EQQ*IKNTTHPPE

Sequence BCLAF1.aAug05

ACGCCCCAGATCGGAAGTGACGGAGACGTGCTAGCGCGTCGAAGGTAGCTCTATGGTTTTCCTCGCGTTCTTGAGTCGGGAAATGGCCGCTGTGTGGTTG CAACGGAGATAAATTCCCGGAACCGCGATTCGGCGTGTCAGGAATTCGAATTTAGAGTTTAATTTCTCAGAGCATTCTCTCCAGGAAGAATTTTTACAGT ATCTCAAAGACTTCACTTGACTTCTTGATCCTGCATAAAACCAAGGAGAAAAGAAATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCT AGATCACAGTCTAGTTCTCGATCAAGATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGTTCCAGAACATATTCAAGGTCTCGTAGTA GAGATCGTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTATTATCA AGGAGGAGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCA AAAAGAAGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGATCATCCTCTTCTCGTTCTTCATCCC CATATAGCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAAAACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAAGTAA ATCACAGGAGGAACCGAAAGATACATTTGAACATGACCCATCTGAGTCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGTGATATTTGGCCTGGC CTTTCAGCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTACACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATGCTCA GTACAGTTCACTCTGCAAAAAATACTCCTTCTCAGCATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGATCTAG TCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGTCCTGCAAAGACAATCGCACCACAGAATGCTCCAAGAGATGAGTCT AGGGGCCGTTCCTCGTTTTATCCTGATGGTGGAGATCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCC TGCTTGATAGGGGTAATACCAGGGATAAAGAGGCTTCAAAAGAGAAAGGATCAGAGAAAGGGAGGGCAGAGGGAGAATGGGAAGATCAGGAAGCTCTAGA TTACTTCAGTGATAAAGAGTCTGGAAAACAAAAGTTTAATGATTCAGAAGGGGATGACACAGAGGAGACAGAGGATTATAGACAGTTCAGGAAGTCAGTC CTCGCAGATCAGGGTAAAAGTTTTGCTACTGCATCTCACCGGAATACTGAGGAGGAAGGACTCAAGTACAAGTCCAAAGTTTCACTGAAAGGCAATAGAG AAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTTAAAGAGACTGGATATGTAGTGGAAAGGCCTAGCACTACAAAAGATAAGCACAAAGAAGAAGA CAAAAATTCTGAAAGAATAACAGTAAAGAAAGAAACTCAGTCACCTGAGCAGGTAAAGTCTGAAAAGCTCAAAGACCTCTTTGATTACAGTCCCCCTCTA CACAAGAATCTGGATGCACGAGAAAAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAATGATAGCGAGTGATTCTCACCGTCCTGAAGTCAAAC TCAAAATGGCACCTGTTCCTCTTGATGATTCTAACAGACCTGCTTCCTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAGAAGGA GCAAGAATTCCGATCCATCTTTGACCACATTAAGTTGCCACAGGCCAGCAAAAGCACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCATCAT GTTAAAGAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAAAGAGCC CTGAAATACACAGGAGAATTGACATCTCACCAAGTACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAAAAGGG AGATAAAAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTCCAGG GAATCCAGTGGATCAAGAAAGCAGGAAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGATCATT CTCGATCTTCATCCTCTTCAGCATCACCTTCTTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCACCATGA AATGAAAGAATACTCAGGCTTTGCAGGAGTTAGCCGACCACGAGGAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACA AATACTGGTCCAAACAACTCAAATACTACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTCTTGC ATGACGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAATCAGG TAGCAGTCCTAAATGGACTCATGACAAATACCAAGGGGATGGGATTGTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGC AAGGAAGAAAAGGAATAATAAATATGAAGTAAGATTACAACAGAGCAGAACTTGCACCCACCATTTTTTTTACCTGATTTTTGTTTTCAAATAAGAATGT AAGCATTTTACTTAAATTTTACTGTTTGCAAGTAGTCTATAGAAATTTTGTTTTAAGTCTTCAAATATCTTGAGAAATAGTAGACTGTATGTTGAAAATT GTACTGAAATAAAGTAGAAAATTGTTACGTACCATATTTGTAACTATCAACTTTTAAAACTTTTAACGTTTTTGTTACATGCATTGTAATTCTGCTTTGT CTATAAGATATGGTCAAGTACAGCTCTGTGAAAGTTCTGATTCTCTTCCTTCCCTGTTTGTCAATGTTTTATTCTGAAGTAAACGTTAGCTCTACATATA AATCCTGGAACAGAAATTGTTTATAGAGACTACACTAATTATTTTAACTGTATACATCTGTTTAATTTGAACACACTACATCGTAGGGTGACTGATTTTT GAAGTATACCACAGACAAAAAGTTGTTACTATGGTAAACTAAGCTAGTTTAACACTTGAGCAAATGCTTAAGAAGGAATTAAAAAAAAAAAGCTTTGCCA ATAGCTAAAAAGTACAAGCTATTAAAAATCAGATTGAAAAGTTTTGAGAAAATGTTATTTTTACTGAAAGCAAGCAGTGGCCTATAAAGAACATTCTTAG GAGCCTTTTCTATTTGCGTTCAAAACTGTGTGTTCTCTTTCTATTCCTATTTGATAGTTTGAGTCATGGTCTTAGATATTAGCTATTTGTGAGAGGAAAC TGGTTTGTAACAATACTGCAAATAGAAACCCCATTTCTACTGAACATCCTAGTTTTAAACAGAAGAAAAACTGTAATCCTGGGGTTGGTATGTAGGAGGT CTATCCTGCAGAATAAGTTGATACATTAGTACCTGATTTCATATCTTACATATTTATTTGAGCTGAACATTAGTTTGTAGTGTAACTATTAGTAAAAATA GAGAAACACAGCATACTGTTCATTAATAGTATTTTAAAAAAATTGTTTTTCAAATGTCACCAATAAAAGTTTTGGCAGGAAGCTTGTTGCGGCATTGATC TAACCTTTTTCCCCCCCATTTCAGTTGCAGTTTTTGTAGAATGGCTTTTTCTTTTTCCTCTTAAGAGTTCTATTCTTCAGGTAGATAATTTTTCAAATGT GAATTATCTTTTGTGTCTATATTGATAGCTCTTAAAGGAGTGAAAATCTAAAATAGTAAATTTCAATGTTAAGTGTCTGCTTTATGGGCATATATAAAAG TAGACACATTTCATTTGTTAATTTAGTTGTGTGTGTGTGTTAAAAGGAGCTAATGCTTATTCTGTTAATGTAAACTTTTGAAGATCTTAAGTGTATTGCT CTTTCATCTTAAACACTTTCGAGGATTTGCAGTGCGTCTAGCACCTAGATTACAGCCAGGAACATTGGTTAAGAACTGTTGGAAACAAAACTAAAAGCAA ACTCAACATATGTGATGTTTATGGCCCTCAGATCCTTAGTATTGTGTGATTTTCCCCCGTTAACATGTCTTTCTAAAATTGTCTATTAAAGCAGAGGAAA TACCTGCCAAAGGAAGTATGTATTGCATTAATCAGGGCATAACTAATATTCTCCTGTTCAGAATAATACTTATTTACGTGTGAAAGCAACATGGATGTGA TTCCCAACACAGAATTTTCATGACCCTTTTATTGTATACAAATAAATACCATAACAGTTACTTGGTTAGACATCAAATCTGTGTGCATGACTATGTGCTT ATCCACTTAAGACAATAGGTAAAAGGGGATCTGAGAAATTATGTAATAGGGAGTGGGAATAAAACTACTTAATTCCTGTGGGCAGGTTATATTTTAAGTT CAAATGCATTGCTTTAACCTTTGGTTACTTTTATTCTGTTAAACAGAATTGAAGAAAGAGTATTATACCAGAGTGTAGTAGGCTAGGGTGATTGTAAGAA CTCTGTAATAGAATGTCATTGTGGATGTTACCTTTTTCAGATCCAAGCATATAAAAAGCCTGTATATTTTTTAAAAACACATCTTAACTCCACGCTTTAC GATATTATAAAAGTTGAATGGTTCCTCTTGGTAAGGATATTTGCTTACAAGTGCTAGGAAATAACTCACTGATACCTGCGTTAACATACTTTGTTTTGCC TAGAGAGGGGCAATAAAAATGAACCAAAGGATATTTCCAGAAAGGATTAAGAAAGCTGTTTAAGAAGGCCATGACTCTTTAGGTGTGTATGTGTACCTTT CAGCATCCTAGGAATTTTTATACTAAAAGCAAAATGTTTTTTCCAGTTAGTCTTCTTCAAGGAATTACTATTGTTCCTTTTGTCACAGGTAAAATCAGTG TTGGGAATTATAATTTGAGAAAAATATTACCCAGTAACATTGAATGTAGATGGCTAAACGATTCTTACTCAGTGTGATGTATAATGATGCAACAGGGACC CTTGTAAATTGTCATACGCCAATAAAATGTCACAAGTAATAACTGCTGTTGTTTGTTTACCTGTGTCTATTTCACACATCTTATTTCTGTGGCCTATTTT AGAATATCAGCGCATCTGTTAGGAAGATTACTGGTGTGGTAAGGCTTGATAAATGCTTTATAAACTCCCTCAAACCTTTCTTTACTGTTTTTTTGTTTTG TCTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTTCCCTTGTCTCCCCTGGGAAAATGGGAAATTTTACAGTTGGTAAATCTAAGCCAA AATTATTTTGAAATAAAGGAATTCTGGATGTCCAGTTTAGTCCTCGTTTTCTTACGTTAATCTGGGACCTTATCACCCATAATATGGTGATTACTTCTCT TTCTAAAAACATAGTAGCTAGTAAATAAGTAAAAAGAATTGTCTTTTCATTCACTTTAAGTAAGATGTGGTATAATTCTTACCATGTGCCATCCTGTCAG TTTTAACAAAGCATTTTCACAGAAATTTGTGTACTAAGACAAACTGACACATTTTGACTCATACAAATGGCAAATTAGTCCTTAAAAATTCTGTGAGAGA AATAACTCTGTGTGTACATACATATGCATGTAAAGTGTTGTGTAAGATCATTGGTAGCTTAATTATACTGGATAATTGTAATGTTATATACAAATTTCTT ATATAAAAGTATGCTGCATTATTAACATTTGTTTAACTTTTCTTATTTTTCTTTTTCTGGGTTTCAACTGGAATCCCATTGGTGGGGATGTACCTTAAGT TTCCTTGTGGAATAATAGACTTTCACATCCAGAAAGAGAAGTTCAGAGGCCTTTCTCAATTTACAGAGTTGAAGAGGAAAGGCAAATGGAGGAATAAACT TCATTGTCATTGGGATTAATTTGGCTAGACTCAATCTCTTGAGCCTTAAAATGAGAAAGTAATATCCATTACATGTTTAGAGTTCTCAAATAGTTGATAC TATTATTAAATGTTTCAAATTTTATTGGGGTAGATACTAAAAATTAGTACACTGTCCTAATGAAGTTTAAATGAACAAGTTTTACATTTCTTAGGATTCA GGAATATATGGAATCTAGTTGTATTTAATTGACTTGAGAGCCACAGAGATTGTGTTGATTTATGAGCTGGACTCCTATTGTTTTAGAAACATGCAGTAAT GATTAACTTGTGTCAATGATGGTAATGAGACCAAACTTGTACATTTAATTAAGGGTACCAGTTCTTTTTATGTTACTAGACTATACCTTGGCCTTTAAAA ATGAATCTCACTGATTAAAGAGAACAGGCATTATTAGGATAGCATTCCACCACAACTAGAAACATTCAAATAATGTGTCTTAATTGTAATCTGTATATAG GAAAATTTTTCCTATGGATATTTTTGGTGTTTTACCACAGTGAACTGATTTGTAGCACTTATGAAGTGCAGAAGGTAATATTCTTGAAAATAGAAAAAGG TTGGGTGAGCAGGCTTTAATGCCTTTCCCCCAAGAATATACATCGAATTTTTCTTAATCTTTTGGGGTTGGCCAGCTTCCAGATTTCATTAATAATGAGC TCTGCCTTTAATAAAAGTACATGATCATAGCTACACTGTATGTTTAGGTGGTGTGAAATGATTTATAATCACAGCTTGAACTGTGTTTGCTTGGTACTGT CATAGTGATTACAAATTTCATGGAATGCGAAGAGCAACAATAAATAAAAAATACCACTCACCCTCCAGAA

NM_014739-3 (forward primer)
NM_014739-4 (reverse primer)
Single exon skipping

Frame 0

TPQIGSDGDVLARRR*LYGFPRVLESGNGRCVV ATEINSRNRDSACQEFEFRV*FLRAFSPGRIFTV SQRLHLTS*SCIKPRRKEMGRSNSRSHSSRSKS RSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRS RDRMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQ GGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSP KRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSS PYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSK SQEEPKDTFEHDPSESIDEFNKSSATSGDIWPG LSAYDNSPRSPHSPSPIATPPSQSSSCSDAPML STVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSS RYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDES RGRSSFYPDGGDQETAKTGKFLKRFTDEESRVF LLDRGNTRDKEASKEKGSEKGRAEGEWEDQEALD YFSDKESGKQKFNDSEGDDTEETEDYRQFRKSV LADQGKSFATASHRNTEEEGLKYKSKVSLKGNR ESDGFREEKNYKLKETGYVVERPSTTKDKHKEED KNSERITVKKETQSPEQVKSEKLKDLFDYSPPL HKNLDAREKSTFREESPLRIKMIASDSHRPEVK LKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKE QEFRSIFDHIKLPQASKSTSESFIQHIVSLVHH VKEQYFKSAAMTLNERFTSYQKATEEHSTRQKS PEIHRRIDISPSTLRKHTRLAGEERVFKEENQKG DKKLRCDSADLRHDIDRRRKERSKERGDSKGSR ESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDH SRSSSSSASPSSPSSREEKESKKEREEEFKTHHE MKEYSGFAGVSRPRGTFFRIRGRGRARGVFAGT NTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFL HDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSG SSPKWTHDKYQGDGIVEDEEETMENNEEKKDRR KEEKE**I*SKITTEQNLHPPFFLPDFCFQIRM *AFYLNFTVCK*SIEILF*VFKYLEK**TVC*KL Y*NKVENCYVPYL*LSTFKTFNVFVTCIVILLC L*DMVKYSSVKVLILFLPCLSMFYSEVNVSSTY KSWNRNCL*RLH*LF*LYTSV*FEHTTS*GD*FL KYTTDKKLLLW*TKLV*HLSKCLRRN*KKKALP IAKKYKLLKIRLKSFEKMLFLLKASSGL*RTFL GAFSICVQNCVFSFYSYLIV*VMVLDISYL*EET GL*QYCK*KPHFY*TS*F*TEEKL*SWGWYVGG LSCRIS*YIST*FHILHIYLS*TLVCSVTISKN RETQHTVH**YFKKIVFQMSPIKVLAGSLLRH*S NLFPPHFSCSFCRMAFSFSS*EFYSSGR*FFKC ELSFVSILIALKGVKI*NSKFQC*VSALWAYIK VDTFHLLI*LCVCVKRS*CLFC*CKLLKILSVLL FHLKHFRGFAVRLAPRLQPGTLVKNCWKQN*KQ TQHM*CLWPSDP*YCVIFPR*HVFLKLSIKAEE IPAKGSMYCINQGITNILLFRIILIYV*KQHGCD SQHRIFMTLLLYTNKYHNSYLVRHQICVHDYVL IHLRQ*VKGDLRNYVIGSGNKTT*FLWAGYILS SNALL*PLVTFILLNRIEERVLYQSVVG*GDCKN SVIECHCGCYLFQIQAYKKPVYFLKTHLNSTLY DIIKVEWFLLVRIFAYKC*EITH*YLR*HTLFC LERGNKNEPKDISRKD*ESCLRRP*LFRCVCVPF SILGIFILKAKCFFQLVFFKELLLFLLSQVKSV LGIII*EKYYPVTLNVDG*TILTQCDV**CNRD PCKLSYANKMSQVITAVVCLPVSISHILFLWPIL EYQRIC*EDYWCGKA**MLYKLPQTFLYCFFVL SCFCFCFCFCFCFCFSLVSPGKMGNFTVGKSKP KLF*NKGILDVQFSPRFLTLIWDLITHNMVITSL SKNIVASK*VKRIVFSFTLSKMWYNSYHVPSCQ F*QSIFTEICVLRQTDTF*LIQMAN*SLKIL*E K*LCVYIHMHVKCCVRSLVA*LYWIIVMLYTNFL YKSMLHY*HLFNFSYFSFSGFQLESHWWGCTLS FLVE**TFTSRKRSSEAFLNLQS*RGKANGGIN FIVIGINLARLNLLSLKMRK*YPLHV*SSQIVDT IIKCFKFYWGRY*KLVHCPNEV*MNKFYIS*DS GIYGI*LYLIDLRATEIVLIYELDSYCFRNMQ* *LTCVNDGNETKLVHLIKGTSSFYVTRLYLGL*K *ISLIKENRHY*DSIPPQLETFK*CVLIVICI* ENFSYGYFWCFTTVN*FVALMKCRR*YS*K*KK VG*AGFNAFPPRIYIEFFLIFWGWPASRFH***A LPLIKVHDHSYTVCLGGVK*FIITA*TVFAWYC HSDYKFHGMRRATINKKYHSPSR

Frame 1

RPRSEVTETC*RVEGSSMVFLAFLSREMAAVWL QRR*IPGTAIRRVRNSNLEFNFSEHSLQEEFLQ YLKDFT*LLDPA*NQGEKKWVAPILDHILQGQSL DHSLVLDQDQDLILERSDTVLGLVPEHIQGLVV EIVCILEIIVAITEIIEE*DDLMGTEEGVEGII KEEEVDIIEVVIDLSGIEGTLGVLDEVVHVPGVQ KEDPFLLKDPEADLAGHIDLLGLQDHPLLVLHP HIANLLFLKDEGLRKNKPKKLKGNPKKRVR*KV NHRRNRKIHLNMTHLSLSMNLISHQPHPVIFGLA FQLMIIVLDHPIVLHLLLHHLVRVHLALMLPCS VQFTLQKILLLSIHIPFSIVLKGLGLVLLEMDL VDTVLLRIVQFITSLHEEVLQRQSHHRMLQEMSL GAVPRFILMVEIRKLQRLGSS*KGSQMKSLEYS CLIGVIPGIKRLQKRKDQRKGGQRENGKIRKL* ITSVIKSLENKSLMIQKGMTQRRQRIIDSSGSQS SQIRVKVLLLHLTGILRRKDSSTSPKFH*KAIE KVMDLEKKKIINLKRLDM*WKGLALQKISTKKK TKILKE*Q*RKKLSHLSR*SLKSSKTSLITVPLY TRIWMHEKSLPSERKAHLGSK**RVILTVLKSN SKWHLFLLMILTDLLP*LKTGCLLVHLSILSRR SKNSDPSLTTLSCHRPAKALQSHLFNTLCPWFIM LKSNTSSQLQ*P*TSGSLRIRKPLKNIVLGKRA LKYTGELTSHQVP*GSIPV*QGKREFLKKKIKR EIKN*GVTLLTFGMTLIAVEKKEVKNGEIPRAPG NPVDQESRKKLQKITRNTNLTKMTVNIKESKII LDLHPLQHHLLLPVLEKKRRVRRKEKKNLKLTM K*KNTQALQELADHEEPFFELEAEEEPEEFLLGQ ILVQTTQILLFKRDRRKRNGIQNIPQRARSTSC MTTEMMVWIIGPKEEEVVVLFNVAEGALTSKNQ VAVLNGLMTNTKGMGLLKMKKRPWKIMKKRRTDA RKKRNNKYEVRLQQSRTCTHHFFYLIFVFK*EC KHFT*ILLFASSL*KFCFKSSNILRNSRLYVEN CTEIK*KIVTYHICNYQLLKLLTFLLHAL*FCFV YKIWSSTAL*KF*FSSFPVCQCFILK*TLALHI NPGTEIVYRDYTNYFNCIHLFNLNTLHRRVTDF *SIPQTKSCYYGKLS*FNT*ANA*EGIKKKKLCQ *LKSTSY*KSD*KVLRKCYFY*KQAVAYKEHS* EPFLFAFKTVCSLSIPI**FESWS*ILAICERK LVCNNTANRNPISTEHPSFKQKKNCNPGVGM*EV YPAE*VDTLVPDFISYIFI*AEH*FVV*LLVKI EKHSILFINSILKKLFFKCHQ*KFWQEACCGID LTFFPPISVAVFVEWLFLFPLKSSILQVDNFSNV NYLLCLY**LLKE*KSKIVNFNVKCLLYGHI*K *THFIC*FSCVCVLKGANAYSVNVNF*RS*VYC SFILNTFEDLQCV*HLDYSQEHWLRTVGNKTKSK LNICDVYGPQILSIV*FSPVNMSF*NCLLKQRK YLPKEVCIALIRA*LIFSCSE*YLFTCESNMDV IPNTEFS*PFYCIQINTITVTWLDIKSVCMTMCL ST*DNR*KGI*EIM**GVGIKLLNSCGQVIF*V QMHCFNLWLLLFC*TELKKEYYTRV**ARVIVR TL**NVIVDVTFFRSKHIKSLYIF*KHILTPRFT IL*KLNGSSW*GYLLTSARK*LTDTCVNILCFA *RGAIKMNQRIFPERIKKAV*EGHDSLGVYVYL SAS*EFLY*KQNVFSS*SSSRNYYCSFCHR*NQC WEL*FEKNITQ*H*M*MAKRFLLSVMYNDATGT LVNCHTPIKCHK**LLLFVYLCLFHTSYFCGLF *NISASVRKITGVVRLDKCFINSLKPFFTVFLFC LVFVFVFVFVFVFVFPLSPLGKWEILQLVNLSQ NYFEIKEFWMSSLVLVFLR*SGTLSPIIW*LLL FLKT**LVNK*KELSFHSL*VRCGIILTMCHPVS FNKAFSQKFVY*DKLTHFDSYKWQISP*KFCER NNSVCTYICM*SVV*DHW*LNYTG*L*CYIQIS YIKVCCIINICLTFLIFLFLGFNWNPIGGDVP*V SLWNNRLSHPEREVQRPFSIYRVEEERQMEE*T SLSLGLIWLDSIS*ALK*ESNIHYMFRVLK*LI LLLNVSNFIGVDTKN*YTVLMKFK*TSFTFLRIQ EYMESSCI*LT*EPQRLC*FMSWTPIVLETCSN D*LVSMMVMRPNLYI*LRVPVLFMLLDYTLAFK NESH*LKRTGIIRIAFHHN*KHSNNVS*L*SVYR KIFPMDIFGVLPQ*TDL*HL*SAEGNILENRKR LGEQALMPFPQEYTSNFS*SFGVGQLPDFINNE LCL**KYMIIATLYV*VV*NDL*SQLELCLLGTV IVITNFMECEEQQ*IKNTTHPPE

Frame 2

APDRK*RRRASASKVALWFSSRS*VGKWPLCGC NGDKFPEPRFGVSGIRI*SLISQSILSRKNFYS ISKTSLDFLILHKTKEKRNGSLQF*ITFFKVKV *ITV*FSIKIKISF*KEAIQF*VSFQNIFKVS** RSYVF*RLSSRLQK**RNETTLWVQRKG*RVLS RRRR*ISSRWL*TCLE*KAL*ES*TRSFTFQES KKKIRFFSKIQKQISPVI*IF*VSKIILFSFFIP I*QISCF*KTRVSGKTNQKS*RGTPRRESVEK* ITGGTERYI*T*PI*VYR*I**VISHIR*YLAW PFSL***S*ITP*SFTYCYTT*SEFILL*CSHAQ YSSLCKKYSFSAFTFHSA*S*KVWVWFCWKWI* SIQSFSE*SNSSHPFTKKSCKDNRTTECSKR*V *GPFLVLS*WWRSGNCKDWEVLKKVHR*RV*SIP A**G*YQG*RGFKRERIREREGRGRMGRSGSSR LLQ**RVWKTKV**FRRG*HRGDRGL*TVQEVS PRRSG*KFCYCISPEY*GGRTQVQVQSFTERQ*R K*WI*RRKKL*T*RDWICSGKA*HYKR*AQRRR QKF*KNNSKERNSVT*AGKV*KAQRPL*LQSPS TQESGCTRKVYLQRGKPT*DQNDSE*FSPS*SQT QNGTCSS**F*QTCFLD*RQAAC*YTCPFCQEG ARIPIHL*PH*VATGQQKHFRVIYSTHCVLGSS C*RAILQVSCNDPKRAVHFVSESH*RT*YSAKEP *NTQEN*HLTKYPEEAYPFSRGRESF*RRKSKG R*KIKV*LC*PSA*H*SP*KRKK*RTGRFQGLQ GIQWIKKAGKNSKRLQGIQILQR*Q*T*KRARSF SIFILFSITFFSQFSRRKGE*EGKRRRI*NSP* NERILRLCRS*PTTRNLFSN*RQRKSQRSFCWD KYWSKQLKYYFSKETEGRGMGSRIYPKEQEVLLA *RQR*WCGLLGQKRKRSWYFSTWQRAL*LQKIR *QS*MDS*QIPRGWDC*R*RRDHGK**RKEGQT QGRKGIINMK*DYNRAELAPTIFFT*FLFSNKNV SILLKFYCLQVVYRNFVLSLQIS*EIVDCMLKI VLK*SRKLLRTIFVTINF*NF*RFCYMHCNSAL SIRYGQVQLCESSDSLPSLFVNVLF*SKR*LYI* ILEQKLFIETTLIILTVYICLI*THYIVG*LIF EVYHRQKVVTMVN*ASLTLEQMLKKELKKKSFA NS*KVQAIKNQIEKF*ENVIFTESKQWPIKNILR SLFYLRSKLCVLFLFLFDSLSHGLRY*LFVRGN WFVTILQIETPFLLNILVLNRRKTVILGLVCRR SILQNKLIH*YLISYLTYLFELNISL*CNY**K* RNTAYCSLIVF*KNCFSNVTNKSFGRKLVAALI *PFSPPFQLQFL*NGFFFFLLRVLFFR*IIFQM *IIFCVYIDSS*RSENLK**ISMLSVCFMGIYKS RHISFVNLVVCVC*KELMLILLM*TFEDLKCIA LSS*TLSRICSASST*ITARNIG*ELLETKLKA NSTYVMFMALRSLVLCDFPPLTCLSKIVY*SRGN TCQRKYVLH*SGHN*YSPVQNNTYLRVKATWM* FPTQNFHDPFIVYK*IP*QLLG*TSNLCA*LCA YPLKTIGKRGSEKLCNREWE*NYLIPVGRLYFKF KCIALTFGYFYSVKQN*RKSIIPECSRLG*L*E LCNRMSLWMLPFSDPSI*KACIFFKNTS*LHAL RYYKS*MVPLGKDICLQVLGNNSLIPALTYFVLP REGQ*K*TKGYFQKGLRKLFKKAMTL*VCMCTF QHPRNFYTKSKMFFPVSLLQGITIVPFVTGKIS VGNYNLRKILPSNIECRWLNDSYSV*CIMMQQGP L*IVIRQ*NVTSNNCCCLFTCVYFTHLISVAYF RISAHLLGRLLVW*GLINAL*TPSNLSLLFFCF VLFLFLFLFLFLFLFFPCLPWENGKFYSW*I*AK IILK*RNSGCPV*SSFSYVNLGPYHP*YGDYFS F*KHSS**ISKKNCLFIHFK*DVV*FLPCAILS VLTKHFHRNLCTKTN*HILTHTNGKLVLKNSVRE ITLCVHTYACKVLCKIIGSLIILDNCNVIYKFL I*KYAALLTFV*LFLFFFFWVSTGIPLVGMYLK FPCGIIDFHIQKEKFRGLSQFTELKRKGKWRNKL HCHWD*FG*TQSLEP*NEKVISITCLEFSNS*Y YY*MFQILLG*ILKISTLS**SLNEQVLHFLGF RNIWNLVVFN*LESHRDCVDL*AGLLLF*KHAVM INLCQ*W**DQTCTFN*GYQFFLCY*TIPWPLK MNLTD*REQALLG*HSTTTRNIQIMCLNCNLYI GKFFLWIFLVFYHSELICSTYEVQKVIFLKIEKG WVSRL*CLSPKNIHRIFLNLLGLASFQISLIMS SAFNKST*S*LHCMFRWCEMIYNHSLNCVCLVL S**LQISWNAKSNNK*KIPLTLQ

Sequence BCLAF1.cAug05

ACGCCCCAGATCGGAAGTGACGGAGACGTGCTAGCGCGTCGAAGGTAGCTCTATGGTTTTCCTCGCGTTCTTGAGTCGGGAAATGGCCGCTGTGTGGTTG CAACGGAGATAAATTCCCGGAACCGCGATTCGGCGTGTCAGGAATTCGAATTTAGAGTTTAATTTCTCAGAGCATTCTCTCCAGGAAGAATTTTTACAGT ATCTCAAAGACTTCACTTGACTTCTTGATCCTGCATAAAACCAAGGAGAAAAGAAATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCT AGATCACAGTCTAGTTCTCGATCAAGATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGTTCCAGAACATATTCAAGGTCTCGTAGTA GAGATCGTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAATAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTATTATCA AGGAGGAGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCA AAAAGAAGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGATCATCCTCTTCTCGTTCTTCATCCC CATATAGCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAAAACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAAGTAA ATCACAGGAGGAACCGAAAGATACATTTGAACATGACCCATCTGAGTCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGTGATATTTGGCCTGGC CTTTCAGCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTACACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATGCTCA GTACAGTTCACTCTGCAAAAAATACTCCTTCTCAGCATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGATCTAG TCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGTCCTGCAAAGACAATCGCACCACAGAATGCTCCAAGAGATGAGTCT AGGGGCCGTTCCTCGTTTTATCCTGATGGTGGAGATCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGTCCCCCTCTACACAAGAATCTGGATGCAC GAGAAAAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAATGATAGCGAGTGATTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCC TCTTGATGATTCTAACAGACCTGCTTCCTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAGAAGGAGCAAGAATTCCGATCCATC TTTGACCACATTAAGTTGCCACAGGCCAGCAAAAGCACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCATCATGTTAAAGAGCAATACTTCA AGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAAAGAGCCCTGAAATACACAGGAGAAT TGACATCTCACCAAGTACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAAAAGGGAGATAAAAAATTAAGGTGT GACTCTGCTGACCTTCGGCATGACATTGATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTCCAGGGAATCCAGTGGATCAAGAA AGCAGGAAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGATCATTCTCGATCTTCATCCTCTTC AGCATCACCTTCTTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCACCATGAAATGAAAGAATACTCAGGC TTTGCAGGAGTTAGCCGACCACGAGGAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAACAACT CAAATACTACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTCTTGCATGACGACAGAGATGATGG TGTGGATTATTGGGCCAAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAATCAGGTAGCAGTCCTAAATGGACT CATGACAAATACCAAGGGGATGGGATTGTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAGAAAAGGAATAAT AAATATGAAGTAAGATTACAACAGAGCAGAACTTGCACCCACCATTTTTTTTACCTGATTTTTGTTTTCAAATAAGAATGTAAGCATTTTACTTAAATTT TACTGTTTGCAAGTAGTCTATAGAAATTTTGTTTTAAGTCTTCAAATATCTTGAGAAATAGTAGACTGTATGTTGAAAATTGTACTGAAATAAAGTAGAA AATTGTTACGTACCATATTTGTAACTATCAACTTTTAAAACTTTTAACGTTTTTGTTACATGCATTGTAATTCTGCTTTGTCTATAAGATATGGTCAAGT ACAGCTCTGTGAAAGTTCTGATTCTCTTCCTTCCCTGTTTGTCAATGTTTTATTCTGAAGTAAACGTTAGCTCTACATATAAATCCTGGAACAGAAATTG TTTATAGAGACTACACTAATTATTTTAACTGTATACATCTGTTTAATTTGAACACACTACATCGTAGGGTGACTGATTTTTGAAGTATACCACAGACAAA AAGTTGTTACTATGGTAAACTAAGCTAGTTTAACACTTGAGCAAATGCTTAAGAAGGAATTAAAAAAAAAAAGCTTTGCCAATAGCTAAAAAGTACAAGC TATTAAAAATCAGATTGAAAAGTTTTGAGAAAATGTTATTTTTACTGAAAGCAAGCAGTGGCCTATAAAGAACATTCTTAGGAGCCTTTTCTATTTGCGT TCAAAACTGTGTGTTCTCTTTCTATTCCTATTTGATAGTTTGAGTCATGGTCTTAGATATTAGCTATTTGTGAGAGGAAACTGGTTTGTAACAATACTGC AAATAGAAACCCCATTTCTACTGAACATCCTAGTTTTAAACAGAAGAAAAACTGTAATCCTGGGGTTGGTATGTAGGAGGTCTATCCTGCAGAATAAGTT GATACATTAGTACCTGATTTCATATCTTACATATTTATTTGAGCTGAACATTAGTTTGTAGTGTAACTATTAGTAAAAATAGAGAAACACAGCATACTGT TCATTAATAGTATTTTAAAAAAATTGTTTTTCAAATGTCACCAATAAAAGTTTTGGCAGGAAGCTTGTTGCGGCATTGATCTAACCTTTTTCCCCCCCAT TTCAGTTGCAGTTTTTGTAGAATGGCTTTTTCTTTTTCCTCTTAAGAGTTCTATTCTTCAGGTAGATAATTTTTCAAATGTGAATTATCTTTTGTGTCTA TATTGATAGCTCTTAAAGGAGTGAAAATCTAAAATAGTAAATTTCAATGTTAAGTGTCTGCTTTATGGGCATATATAAAAGTAGACACATTTCATTTGTT AATTTAGTTGTGTGTGTGTGTTAAAAGGAGCTAATGCTTATTCTGTTAATGTAAACTTTTGAAGATCTTAAGTGTATTGCTCTTTCATCTTAAACACTTT CGAGGATTTGCAGTGCGTCTAGCACCTAGATTACAGCCAGGAACATTGGTTAAGAACTGTTGGAAACAAAACTAAAAGCAAACTCAACATATGTGATGTT TATGGCCCTCAGATCCTTAGTATTGTGTGATTTTCCCCCGTTAACATGTCTTTCTAAAATTGTCTATTAAAGCAGAGGAAATACCTGCCAAAGGAAGTAT GTATTGCATTAATCAGGGCATAACTAATATTCTCCTGTTCAGAATAATACTTATTTACGTGTGAAAGCAACATGGATGTGATTCCCAACACAGAATTTTC ATGACCCTTTTATTGTATACAAATAAATACCATAACAGTTACTTGGTTAGACATCAAATCTGTGTGCATGACTATGTGCTTATCCACTTAAGACAATAGG TAAAAGGGGATCTGAGAAATTATGTAATAGGGAGTGGGAATAAAACTACTTAATTCCTGTGGGCAGGTTATATTTTAAGTTCAAATGCATTGCTTTAACC TTTGGTTACTTTTATTCTGTTAAACAGAATTGAAGAAAGAGTATTATACCAGAGTGTAGTAGGCTAGGGTGATTGTAAGAACTCTGTAATAGAATGTCAT TGTGGATGTTACCTTTTTCAGATCCAAGCATATAAAAAGCCTGTATATTTTTTAAAAACACATCTTAACTCCACGCTTTACGATATTATAAAAGTTGAAT GGTTCCTCTTGGTAAGGATATTTGCTTACAAGTGCTAGGAAATAACTCACTGATACCTGCGTTAACATACTTTGTTTTGCCTAGAGAGGGGCAATAAAAA TGAACCAAAGGATATTTCCAGAAAGGATTAAGAAAGCTGTTTAAGAAGGCCATGACTCTTTAGGTGTGTATGTGTACCTTTCAGCATCCTAGGAATTTTT ATACTAAAAGCAAAATGTTTTTTCCAGTTAGTCTTCTTCAAGGAATTACTATTGTTCCTTTTGTCACAGGTAAAATCAGTGTTGGGAATTATAATTTGAG AAAAATATTACCCAGTAACATTGAATGTAGATGGCTAAACGATTCTTACTCAGTGTGATGTATAATGATGCAACAGGGACCCTTGTAAATTGTCATACGC CAATAAAATGTCACAAGTAATAACTGCTGTTGTTTGTTTACCTGTGTCTATTTCACACATCTTATTTCTGTGGCCTATTTTAGAATATCAGCGCATCTGT TAGGAAGATTACTGGTGTGGTAAGGCTTGATAAATGCTTTATAAACTCCCTCAAACCTTTCTTTACTGTTTTTTTGTTTTGTCTTGTTTTTGTTTTTGTT TTTGTTTTTGTTTTTGTTTTTGTTTTTCCCTTGTCTCCCCTGGGAAAATGGGAAATTTTACAGTTGGTAAATCTAAGCCAAAATTATTTTGAAATAAAGG AATTCTGGATGTCCAGTTTAGTCCTCGTTTTCTTACGTTAATCTGGGACCTTATCACCCATAATATGGTGATTACTTCTCTTTCTAAAAACATAGTAGCT AGTAAATAAGTAAAAAGAATTGTCTTTTCATTCACTTTAAGTAAGATGTGGTATAATTCTTACCATGTGCCATCCTGTCAGTTTTAACAAAGCATTTTCA CAGAAATTTGTGTACTAAGACAAACTGACACATTTTGACTCATACAAATGGCAAATTAGTCCTTAAAAATTCTGTGAGAGAAATAACTCTGTGTGTACAT ACATATGCATGTAAAGTGTTGTGTAAGATCATTGGTAGCTTAATTATACTGGATAATTGTAATGTTATATACAAATTTCTTATATAAAAGTATGCTGCAT TATTAACATTTGTTTAACTTTTCTTATTTTTCTTTTTCTGGGTTTCAACTGGAATCCCATTGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGA CTTTCACATCCAGAAAGAGAAGTTCAGAGGCCTTTCTCAATTTACAGAGTTGAAGAGGAAAGGCAAATGGAGGAATAAACTTCATTGTCATTGGGATTAA TTTGGCTAGACTCAATCTCTTGAGCCTTAAAATGAGAAAGTAATATCCATTACATGTTTAGAGTTCTCAAATAGTTGATACTATTATTAAATGTTTCAAA TTTTATTGGGGTAGATACTAAAAATTAGTACACTGTCCTAATGAAGTTTAAATGAACAAGTTTTACATTTCTTAGGATTCAGGAATATATGGAATCTAGT TGTATTTAATTGACTTGAGAGCCACAGAGATTGTGTTGATTTATGAGCTGGACTCCTATTGTTTTAGAAACATGCAGTAATGATTAACTTGTGTCAATGA TGGTAATGAGACCAAACTTGTACATTTAATTAAGGGTACCAGTTCTTTTTATGTTACTAGACTATACCTTGGCCTTTAAAAATGAATCTCACTGATTAAA GAGAACAGGCATTATTAGGATAGCATTCCACCACAACTAGAAACATTCAAATAATGTGTCTTAATTGTAATCTGTATATAGGAAAATTTTTCCTATGGAT ATTTTTGGTGTTTTACCACAGTGAACTGATTTGTAGCACTTATGAAGTGCAGAAGGTAATATTCTTGAAAATAGAAAAAGGTTGGGTGAGCAGGCTTTAA TGCCTTTCCCCCAAGAATATACATCGAATTTTTCTTAATCTTTTGGGGTTGGCCAGCTTCCAGATTTCATTAATAATGAGCTCTGCCTTTAATAAAAGTA CATGATCATAGCTACACTGTATGTTTAGGTGGTGTGAAATGATTTATAATCACAGCTTGAACTGTGTTTGCTTGGTACTGTCATAGTGATTACAAATTTC ATGGAATGCGAAGAGCAACAATAAATAAAAAATACCACTCACCCTCCAGAA

NM_014739-3 (forward primer)
NM_014739-4 (reverse primer)
Single exon skipping

Frame 0

TPQIGSDGDVLARRR*LYGFPRVLESGNGRCVV ATEINSRNRDSACQEFEFRV*FLRAFSPGRIFTV SQRLHLTS*SCIKPRRKEMGRSNSRSHSSRSKS RSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRS RDRMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQ GGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSP KRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSS PYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSK SQEEPKDTFEHDPSESIDEFNKSSATSGDIWPG LSAYDNSPRSPHSPSPIATPPSQSSSCSDAPML STVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSS RYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDES RGRSSFYPDGGDQETAKTGKFLKSPPLHKNLDA REKSTFREESPLRIKMIASDSHRPEVKLKMAPVP LDDSNRPASLTKDRLLASTLVHSVKKEQEFRSI FDHIKLPQASKSTSESFIQHIVSLVHHVKEQYF KSAAMTLNERFTSYQKATEEHSTRQKSPEIHRRI DISPSTLRKHTRLAGEERVFKEENQKGDKKLRC DSADLRHDIDRRRKERSKERGDSKGSRESSGSR KQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSS ASPSSPSSREEKESKKEREEEFKTHHEMKEYSG FAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNN SNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDG VDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWT HDKYQGDGIVEDEEETMENNEEKKDRRKEEKE* *I*SKITTEQNLHPPFFLPDFCFQIRM*AFYLNF TVCK*SIEILF*VFKYLEK**TVC*KLY*NKVE NCYVPYL*LSTFKTFNVFVTCIVILLCL*DMVK YSSVKVLILFLPCLSMFYSEVNVSSTYKSWNRNC L*RLH*LF*LYTSV*FEHTTS*GD*FLKYTTDK KLLLW*TKLV*HLSKCLRRN*KKKALPIAKKYK LLKIRLKSFEKMLFLLKASSGL*RTFLGAFSICV QNCVFSFYSYLIV*VMVLDISYL*EETGL*QYC K*KPHFY*TS*F*TEEKL*SWGWYVGGLSCRIS *YIST*FHILHIYLS*TLVCSVTISKNRETQHTV H**YFKKIVFQMSPIKVLAGSLLRH*SNLFPPH FSCSFCRMAFSFSS*EFYSSGR*FFKCELSFVS ILIALKGVKI*NSKFQC*VSALWAYIKVDTFHLL I*LCVCVKRS*CLFC*CKLLKILSVLLFHLKHF RGFAVRLAPRLQPGTLVKNCWKQN*KQTQHM*C LWPSDP*YCVIFPR*HVFLKLSIKAEEIPAKGSM YCINQGITNILLFRIILIYV*KQHGCDSQHRIF MTLLLYTNKYHNSYLVRHQICVHDYVLIHLRQ* VKGDLRNYVIGSGNKTT*FLWAGYILSSNALL*P LVTFILLNRIEERVLYQSVVG*GDCKNSVIECH CGCYLFQIQAYKKPVYFLKTHLNSTLYDIIKVE WFLLVRIFAYKC*EITH*YLR*HTLFCLERGNKN EPKDISRKD*ESCLRRP*LFRCVCVPFSILGIF ILKAKCFFQLVFFKELLLFLLSQVKSVLGIII* EKYYPVTLNVDG*TILTQCDV**CNRDPCKLSYA NKMSQVITAVVCLPVSISHILFLWPILEYQRIC *EDYWCGKA**MLYKLPQTFLYCFFVLSCFCFC FCFCFCFCFSLVSPGKMGNFTVGKSKPKLF*NKG ILDVQFSPRFLTLIWDLITHNMVITSLSKNIVA SK*VKRIVFSFTLSKMWYNSYHVPSCQF*QSIF TEICVLRQTDTF*LIQMAN*SLKIL*EK*LCVYI HMHVKCCVRSLVA*LYWIIVMLYTNFLYKSMLH Y*HLFNFSYFSFSGFQLESHWWGCTLSFLVE** TFTSRKRSSEAFLNLQS*RGKANGGINFIVIGIN LARLNLLSLKMRK*YPLHV*SSQIVDTIIKCFK FYWGRY*KLVHCPNEV*MNKFYIS*DSGIYGI* LYLIDLRATEIVLIYELDSYCFRNMQ**LTCVND GNETKLVHLIKGTSSFYVTRLYLGL*K*ISLIK ENRHY*DSIPPQLETFK*CVLIVICI*ENFSYG YFWCFTTVN*FVALMKCRR*YS*K*KKVG*AGFN AFPPRIYIEFFLIFWGWPASRFH***ALPLIKV HDHSYTVCLGGVK*FIITA*TVFAWYCHSDYKF HGMRRATINKKYHSPSR

Frame 1

RPRSEVTETC*RVEGSSMVFLAFLSREMAAVWL QRR*IPGTAIRRVRNSNLEFNFSEHSLQEEFLQ YLKDFT*LLDPA*NQGEKKWVAPILDHILQGQSL DHSLVLDQDQDLILERSDTVLGLVPEHIQGLVV EIVCILEIIVAITEIIEE*DDLMGTEEGVEGII KEEEVDIIEVVIDLSGIEGTLGVLDEVVHVPGVQ KEDPFLLKDPEADLAGHIDLLGLQDHPLLVLHP HIANLLFLKDEGLRKNKPKKLKGNPKKRVR*KV NHRRNRKIHLNMTHLSLSMNLISHQPHPVIFGLA FQLMIIVLDHPIVLHLLLHHLVRVHLALMLPCS VQFTLQKILLLSIHIPFSIVLKGLGLVLLEMDL VDTVLLRIVQFITSLHEEVLQRQSHHRMLQEMSL GAVPRFILMVEIRKLQRLGSS*KVPLYTRIWMH EKSLPSERKAHLGSK**RVILTVLKSNSKWHLF LLMILTDLLP*LKTGCLLVHLSILSRRSKNSDPS LTTLSCHRPAKALQSHLFNTLCPWFIMLKSNTS SQLQ*P*TSGSLRIRKPLKNIVLGKRALKYTGE LTSHQVP*GSIPV*QGKREFLKKKIKREIKN*GV TLLTFGMTLIAVEKKEVKNGEIPRAPGNPVDQE SRKKLQKITRNTNLTKMTVNIKESKIILDLHPL QHHLLLPVLEKKRRVRRKEKKNLKLTMK*KNTQA LQELADHEEPFFELEAEEEPEEFLLGQILVQTT QILLFKRDRRKRNGIQNIPQRARSTSCMTTEMM VWIIGPKEEEVVVLFNVAEGALTSKNQVAVLNGL MTNTKGMGLLKMKKRPWKIMKKRRTDARKKRNN KYEVRLQQSRTCTHHFFYLIFVFK*ECKHFT*I LLFASSL*KFCFKSSNILRNSRLYVENCTEIK*K IVTYHICNYQLLKLLTFLLHAL*FCFVYKIWSS TAL*KF*FSSFPVCQCFILK*TLALHINPGTEI VYRDYTNYFNCIHLFNLNTLHRRVTDF*SIPQTK SCYYGKLS*FNT*ANA*EGIKKKKLCQ*LKSTS Y*KSD*KVLRKCYFY*KQAVAYKEHS*EPFLFA FKTVCSLSIPI**FESWS*ILAICERKLVCNNTA NRNPISTEHPSFKQKKNCNPGVGM*EVYPAE*V DTLVPDFISYIFI*AEH*FVV*LLVKIEKHSIL FINSILKKLFFKCHQ*KFWQEACCGIDLTFFPPI SVAVFVEWLFLFPLKSSILQVDNFSNVNYLLCL Y**LLKE*KSKIVNFNVKCLLYGHI*K*THFIC *FSCVCVLKGANAYSVNVNF*RS*VYCSFILNTF EDLQCV*HLDYSQEHWLRTVGNKTKSKLNICDV YGPQILSIV*FSPVNMSF*NCLLKQRKYLPKEV CIALIRA*LIFSCSE*YLFTCESNMDVIPNTEFS *PFYCIQINTITVTWLDIKSVCMTMCLST*DNR *KGI*EIM**GVGIKLLNSCGQVIF*VQMHCFN LWLLLFC*TELKKEYYTRV**ARVIVRTL**NVI VDVTFFRSKHIKSLYIF*KHILTPRFTIL*KLN GSSW*GYLLTSARK*LTDTCVNILCFA*RGAIK MNQRIFPERIKKAV*EGHDSLGVYVYLSAS*EFL Y*KQNVFSS*SSSRNYYCSFCHR*NQCWEL*FE KNITQ*H*M*MAKRFLLSVMYNDATGTLVNCHT PIKCHK**LLLFVYLCLFHTSYFCGLF*NISASV RKITGVVRLDKCFINSLKPFFTVFLFCLVFVFV FVFVFVFVFPLSPLGKWEILQLVNLSQNYFEIK EFWMSSLVLVFLR*SGTLSPIIW*LLLFLKT**L VNK*KELSFHSL*VRCGIILTMCHPVSFNKAFS QKFVY*DKLTHFDSYKWQISP*KFCERNNSVCT YICM*SVV*DHW*LNYTG*L*CYIQISYIKVCCI INICLTFLIFLFLGFNWNPIGGDVP*VSLWNNR LSHPEREVQRPFSIYRVEEERQMEE*TSLSLGL IWLDSIS*ALK*ESNIHYMFRVLK*LILLLNVSN FIGVDTKN*YTVLMKFK*TSFTFLRIQEYMESS CI*LT*EPQRLC*FMSWTPIVLETCSND*LVSM MVMRPNLYI*LRVPVLFMLLDYTLAFKNESH*LK RTGIIRIAFHHN*KHSNNVS*L*SVYRKIFPMD IFGVLPQ*TDL*HL*SAEGNILENRKRLGEQAL MPFPQEYTSNFS*SFGVGQLPDFINNELCL**KY MIIATLYV*VV*NDL*SQLELCLLGTVIVITNF MECEEQQ*IKNTTHPPE

Frame 2

APDRK*RRRASASKVALWFSSRS*VGKWPLCGC NGDKFPEPRFGVSGIRI*SLISQSILSRKNFYS ISKTSLDFLILHKTKEKRNGSLQF*ITFFKVKV *ITV*FSIKIKISF*KEAIQF*VSFQNIFKVS** RSYVF*RLSSRLQK**RNETTLWVQRKG*RVLS RRRR*ISSRWL*TCLE*KAL*ES*TRSFTFQES KKKIRFFSKIQKQISPVI*IF*VSKIILFSFFIP I*QISCF*KTRVSGKTNQKS*RGTPRRESVEK* ITGGTERYI*T*PI*VYR*I**VISHIR*YLAW PFSL***S*ITP*SFTYCYTT*SEFILL*CSHAQ YSSLCKKYSFSAFTFHSA*S*KVWVWFCWKWI* SIQSFSE*SNSSHPFTKKSCKDNRTTECSKR*V *GPFLVLS*WWRSGNCKDWEVLKKSPSTQESGCT RKVYLQRGKPT*DQNDSE*FSPS*SQTQNGTCS S**F*QTCFLD*RQAAC*YTCPFCQEGARIPIH L*PH*VATGQQKHFRVIYSTHCVLGSSC*RAILQ VSCNDPKRAVHFVSESH*RT*YSAKEP*NTQEN *HLTKYPEEAYPFSRGRESF*RRKSKGR*KIKV *LC*PSA*H*SP*KRKK*RTGRFQGLQGIQWIKK AGKNSKRLQGIQILQR*Q*T*KRARSFSIFILF SITFFSQFSRRKGE*EGKRRRI*NSP*NERILR LCRS*PTTRNLFSN*RQRKSQRSFCWDKYWSKQL KYYFSKETEGRGMGSRIYPKEQEVLLA*RQR*W CGLLGQKRKRSWYFSTWQRAL*LQKIR*QS*MD S*QIPRGWDC*R*RRDHGK**RKEGQTQGRKGII NMK*DYNRAELAPTIFFT*FLFSNKNVSILLKF YCLQVVYRNFVLSLQIS*EIVDCMLKIVLK*SR KLLRTIFVTINF*NF*RFCYMHCNSALSIRYGQV QLCESSDSLPSLFVNVLF*SKR*LYI*ILEQKL FIETTLIILTVYICLI*THYIVG*LIFEVYHRQ KVVTMVN*ASLTLEQMLKKELKKKSFANS*KVQA IKNQIEKF*ENVIFTESKQWPIKNILRSLFYLR SKLCVLFLFLFDSLSHGLRY*LFVRGNWFVTIL QIETPFLLNILVLNRRKTVILGLVCRRSILQNKL IH*YLISYLTYLFELNISL*CNY**K*RNTAYC SLIVF*KNCFSNVTNKSFGRKLVAALI*PFSPP FQLQFL*NGFFFFLLRVLFFR*IIFQM*IIFCVY IDSS*RSENLK**ISMLSVCFMGIYKSRHISFV NLVVCVC*KELMLILLM*TFEDLKCIALSS*TL SRICSASST*ITARNIG*ELLETKLKANSTYVMF MALRSLVLCDFPPLTCLSKIVY*SRGNTCQRKY VLH*SGHN*YSPVQNNTYLRVKATWM*FPTQNF HDPFIVYK*IP*QLLG*TSNLCA*LCAYPLKTIG KRGSEKLCNREWE*NYLIPVGRLYFKFKCIALT FGYFYSVKQN*RKSIIPECSRLG*L*ELCNRMS LWMLPFSDPSI*KACIFFKNTS*LHALRYYKS*M VPLGKDICLQVLGNNSLIPALTYFVLPREGQ*K *TKGYFQKGLRKLFKKAMTL*VCMCTFQHPRNF YTKSKMFFPVSLLQGITIVPFVTGKISVGNYNLR KILPSNIECRWLNDSYSV*CIMMQQGPL*IVIR Q*NVTSNNCCCLFTCVYFTHLISVAYFRISAHL LGRLLVW*GLINAL*TPSNLSLLFFCFVLFLFLF LFLFLFLFFPCLPWENGKFYSW*I*AKIILK*R NSGCPV*SSFSYVNLGPYHP*YGDYFSF*KHSS **ISKKNCLFIHFK*DVV*FLPCAILSVLTKHFH RNLCTKTN*HILTHTNGKLVLKNSVREITLCVH TYACKVLCKIIGSLIILDNCNVIYKFLI*KYAA LLTFV*LFLFFFFWVSTGIPLVGMYLKFPCGIID FHIQKEKFRGLSQFTELKRKGKWRNKLHCHWD* FG*TQSLEP*NEKVISITCLEFSNS*YYY*MFQ ILLG*ILKISTLS**SLNEQVLHFLGFRNIWNLV VFN*LESHRDCVDL*AGLLLF*KHAVMINLCQ* W**DQTCTFN*GYQFFLCY*TIPWPLKMNLTD* REQALLG*HSTTTRNIQIMCLNCNLYIGKFFLWI FLVFYHSELICSTYEVQKVIFLKIEKGWVSRL* CLSPKNIHRIFLNLLGLASFQISLIMSSAFNKS T*S*LHCMFRWCEMIYNHSLNCVCLVLS**LQIS WNAKSNNK*KIPLTLQ