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Sequences

Sequence FAS.jAug05

CCTACCCGCGCGCAGGCCAAGTTGCTGAATCAATGGAGCCCTCCCCAACCCGGGCGTTCCCCAGCGAGGCTTCCTTCCCATCCTCCTGACCACCGGGGCT TTTCGTGAGCTCGTCTCTGATCTCGCGCAAGAGTGACACACAGGTGTTCAAAGACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGA GCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGT ACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTA TCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCC TGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAG GAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAA AGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACTTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCAC CACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTC CAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAG CCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTT GGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACT GGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGG AAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAAC ATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTT TTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAAC TCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATT ATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTT CTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCAC CCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCT CAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAA TGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCCCAAGTTTCT AAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAAT GTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTAT AAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTGGTA

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

PTRAQAKLLNQWSPPQPGRSPARLPSHPPDHRG FS*ARL*SRARVTHRCSKTLLGSEGSGLRVTWLE PQGRALARNTP*GQPWLPRRSCLFSRGLVDPLS TELGKLFHFGGLLNNHAGHLDPPTSGSYVCC*I IVQKC*CPSD*HQLQGIGIEEDCYYS*DSELGRP AS*WPILP*ALSSRCEHGIIKECTLTSNTKCKE EGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCR KHRKENQGSHESPTLNPETVAINLSDVDLSKYIT TIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNV QDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKK ANLCTLAEKIQTIILKDITSDSENSNFRNEIQSL V*SEKQQIQF*VYAISV*KDS**LAVNTAWFFT GYILSFISAEEPTYL*IFNIS*FCLQGCLKSSW ENKLHQE*MQWHAKYPNRSVCRG*KIKIMLWHLT YDSVV*M*SVYVSTNVYPQANPTL*INRRSYDL LLKYQLLNRQATLPLNYL**F*RFYHISKLCL* L*EDHIYVKYMYLSAEFK*GSTSKTFAQFIGVIL YNISIVNSHRKH*IIMFDYYICVCILLAQNYLL LSQASKAF*AGEYY*SFATSPFLPWCSS*WPNA PPNMEISPKNT**STKRQDCP*KF*PGLEILTAL RESSFVTCHEPMFAIKDDKIDSYFSPTPENVQ* CPM*NLLQMAAYT*QW*NHHLDLGIALVIPPSF *DLRFSLLLSYV*ISLKVCIKCEF*EIIFIFL*M *TVKIVIN*SRYLEPPKELPFMEDFFAPCVWNY KI*VKVRN*IMFLV

Frame 1

LPARRPSC*INGALPNPGVPQRGFLPILLTTGA FRELVSDLAQE*HTGVQRRFWGVREAVYE*LGW SLRGGHWHGTHPEASPGCPGGAASSPAGWWTRSV RSWGSSFTSEDCSTTMLGIWTLLPLVLTSVARL SSKSVNAQVTDINSKGLELRKTVTTVETQNLEG LHHDGQFCHKPCPPDVNMESSRNAHSPATPSAKR KDPDLTWGGFVFFFCQFH*LFG*RERKYRKHAE STERKTKVLMNLQL*ILKQWQ*IYLMLT*VNIS PLLLES*H*VKLKALFERMVSMKPK*MRSRMTMS KTQQNRKFNCFVIGINFMERKKRMTH*LKISKK PIFVLLQRKFRLSSSRTLLVTQKIQTSEMKSKA WSRVKNNKFSSEYMQLVFEKILNSWL*ILLGFLL GTFYHLLALKSQHICRFLISHDSASKDV*NLVG KTNFIKSKCSGMLSTQIGVYAEDERLRLCSGI* HMIL*YECNQCMLVQMSIHRLTPLYESIEEAMTF C*NISY*TGRPLCL*ITSDNSRDFTIFLNFVYN SEKIIFM*SICI*VQNLNKALPQRPLHSLLVSY YTIFQL*IHIENIKL*CLTIIYVYAFYWLKTTYF FLRHQKHFEQESITRALPPLHFCLGAHLNGLMH PQTWKYHQKILNSPPKGKTALRNSSLVWRY*LL SEKVAL*HVMNPCLQSKMIK*ILIFPPPPKMFNN VPCKTCYKWQLIHSNGKIIIWI*ELLLSYPQVS KI*DSPYYYPTFKYL*KFVLNVNFKK*YLYFCK CKL*R*L*TEADTWNHLKNFHLWRIFLPLVFGII KYR*KYVIK*CFW

Frame 2

YPRAGQVAESMEPSPTRAFPSEASFPSS*PPGL FVSSSLISRKSDTQVFKDASGE*GKRFTSDLAG ASGAGTGTEHTLRPALAAQAELPLLPRVGGPAQ YGVGEALSLRRIAQQPCWASGPSYLWFLRLLLDY RPKVLMPK*LTSTPRDWN*GRLLLQLRLRTWKA CIMMANSAISPVLQM*TWNHQGMHTHQQHQVQR GRIQI*LGVALSSSFANSTNCLGEEKGSTENMQK AQKGKPRFS*ISNFKS*NSGNKFI*C*LE*IYH HYCWSHDTKSS*RLCSKEWCQ*SQNR*DQE*QC PRHSRTESSTAS*LASTSWKERSV*HID*RSQKS QSLYSCRENSDYHPQGHY**LRKFKLQK*NPKL GLE*KTTNSVLSICN*CLKRFLIAGCKYCLVFY WVHFIIY*R*RANIFVDF*YLMILPPRMFKI*LG KQTSSRVNAVAC*VPK*ECMQRMKD*DYALASN I*FCSMNVISVC*YKCLSTG*PHSMNQ*KKL*P FAEISVTEQAGHFASKLPLIILEILPYF*TLFIT LRRSYLCKVYVFECRI*IRLYLKDLCTVYWCHI IQYFNCEFT*KTLNYNV*LLYMCMHFTGSKLPT SFSGIKSILSRRVLLELCHLSIFALVLILMA*CT PKHGNITKKYLIVHQKARLPLEILAWFGDTNCS QRK*LCDMS*THVCNQR**NRFLFFPHPRKCSI MSHVKPATNGSLYIAMVKSSSGFRNCSCHTPKFL RFKILLTTILRLNIFESLY*M*ILRNNIYISVN VNCEDSYKLKQIPGTT*RTSIYGGFFCPLCLEL *NIGKST*LNNVFG

Sequence FAS.aAug05

CCTACCCGCGCGCAGGCCAAGTTGCTGAATCAATGGAGCCCTCCCCAACCCGGGCGTTCCCCAGCGAGGCTTCCTTCCCATCCTCCTGACCACCGGGGCT TTTCGTGAGCTCGTCTCTGATCTCGCGCAAGAGTGACACACAGGTGTTCAAAGACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGA GCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGT ACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTA TCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCC TGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTG CCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAAC TGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATG GAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCC ACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACTTTAAATCCTGAA ACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGA ATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCA TGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATT ACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTT GAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTT TAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGG AGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATC CACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCT CTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAA TAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTG ACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGC CACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAA GACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATG ATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAA TCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCCCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTT TGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAG AACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTGGTA

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

PTRAQAKLLNQWSPPQPGRSPARLPSHPPDHRG FS*ARL*SRARVTHRCSKTLLGSEGSGLRVTWLE PQGRALARNTP*GQPWLPRRSCLFSRGLVDPLS TELGKLFHFGGLLNNHAGHLDPPTSGSYVCC*I IVQKC*CPSD*HQLQGIGIEEDCYYS*DSELGRP AS*WPILP*ALSSR*KES*GLHSQWG*TRLRAL PRREGVHRQSPFFFQMQKM*IV**RTWLRSGNK LHPDPEYQVQM*TKLFL*LYCM*TL*PLHQM*TW NHQGMHTHQQHQVQRGRIQI*LGVALSSSFANS TNCLGEEKGSTENMQKAQKGKPRFS*ISNFKS* NSGNKFI*C*LE*IYHHYCWSHDTKSS*RLCSKE WCQ*SQNR*DQE*QCPRHSRTESSTAS*LASTS WKERSV*HID*RSQKSQSLYSCRENSDYHPQGH Y**LRKFKLQK*NPKLGLE*KTTNSVLSICN*CL KRFLIAGCKYCLVFYWVHFIIY*R*RANIFVDF *YLMILPPRMFKI*LGKQTSSRVNAVAC*VPK* ECMQRMKD*DYALASNI*FCSMNVISVC*YKCLS TG*PHSMNQ*KKL*PFAEISVTEQAGHFASKLP LIILEILPYF*TLFITLRRSYLCKVYVFECRI* IRLYLKDLCTVYWCHIIQYFNCEFT*KTLNYNV* LLYMCMHFTGSKLPTSFSGIKSILSRRVLLELC HLSIFALVLILMA*CTPKHGNITKKYLIVHQKA RLPLEILAWFGDTNCSQRK*LCDMS*THVCNQR* *NRFLFFPHPRKCSIMSHVKPATNGSLYIAMVK SSSGFRNCSCHTPKFLRFKILLTTILRLNIFES LY*M*ILRNNIYISVNVNCEDSYKLKQIPGTT*R TSIYGGFFCPLCLEL*NIGKST*LNNVFG

Frame 1

LPARRPSC*INGALPNPGVPQRGFLPILLTTGA FRELVSDLAQE*HTGVQRRFWGVREAVYE*LGW SLRGGHWHGTHPEASPGCPGGAASSPAGWWTRSV RSWGSSFTSEDCSTTMLGIWTLLPLVLTSVARL SSKSVNAQVTDINSKGLELRKTVTTVETQNLEG LHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPC QEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEIN CTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEH GIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIP LIVWVKRKEVQKTCRKHRKENQGSHESPTLNPE TVAINLSDVDLSKYITTIAGVMTLSQVKGFVRK NGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLH GKKEAYDTLIKDLKKANLCTLAEKIQTIILKDI TSDSENSNFRNEIQSLV*SEKQQIQF*VYAISV *KDS**LAVNTAWFFTGYILSFISAEEPTYL*IF NIS*FCLQGCLKSSWENKLHQE*MQWHAKYPNR SVCRG*KIKIMLWHLTYDSVV*M*SVYVSTNVY PQANPTL*INRRSYDLLLKYQLLNRQATLPLNYL **F*RFYHISKLCL*L*EDHIYVKYMYLSAEFK *GSTSKTFAQFIGVILYNISIVNSHRKH*IIMF DYYICVCILLAQNYLLLSQASKAF*AGEYY*SFA TSPFLPWCSS*WPNAPPNMEISPKNT**STKRQ DCP*KF*PGLEILTALRESSFVTCHEPMFAIKD DKIDSYFSPTPENVQ*CPM*NLLQMAAYT*QW*N HHLDLGIALVIPPSF*DLRFSLLLSYV*ISLKV CIKCEF*EIIFIFL*M*TVKIVIN*SRYLEPPK ELPFMEDFFAPCVWNYKI*VKVRN*IMFLV

Frame 2

YPRAGQVAESMEPSPTRAFPSEASFPSS*PPGL FVSSSLISRKSDTQVFKDASGE*GKRFTSDLAG ASGAGTGTEHTLRPALAAQAELPLLPRVGGPAQ YGVGEALSLRRIAQQPCWASGPSYLWFLRLLLDY RPKVLMPK*LTSTPRDWN*GRLLLQLRLRTWKA CIMMANSAISPVLQVKGKLGTAQSMGMNQTACP AKKGRSTQTKPIFLPNAEDVDCVMKDMA*KWK*T APGPRIPSADVNQTFFVTLLYVNTVTLAPNVNM ESSRNAHSPATPSAKRKDPDLTWGGFVFFFCQF H*LFG*RERKYRKHAESTERKTKVLMNLQL*ILK QWQ*IYLMLT*VNISPLLLES*H*VKLKALFER MVSMKPK*MRSRMTMSKTQQNRKFNCFVIGINF MERKKRMTH*LKISKKPIFVLLQRKFRLSSSRTL LVTQKIQTSEMKSKAWSRVKNNKFSSEYMQLVF EKILNSWL*ILLGFLLGTFYHLLALKSQHICRF LISHDSASKDV*NLVGKTNFIKSKCSGMLSTQIG VYAEDERLRLCSGI*HMIL*YECNQCMLVQMSI HRLTPLYESIEEAMTFC*NISY*TGRPLCL*IT SDNSRDFTIFLNFVYNSEKIIFM*SICI*VQNLN KALPQRPLHSLLVSYYTIFQL*IHIENIKL*CL TIIYVYAFYWLKTTYFFLRHQKHFEQESITRAL PPLHFCLGAHLNGLMHPQTWKYHQKILNSPPKGK TALRNSSLVWRY*LLSEKVAL*HVMNPCLQSKM IK*ILIFPPPPKMFNNVPCKTCYKWQLIHSNGK IIIWI*ELLLSYPQVSKI*DSPYYYPTFKYL*KF VLNVNFKK*YLYFCKCKL*R*L*TEADTWNHLK NFHLWRIFLPLVFGIIKYR*KYVIK*CFW

Sequence FAS.cAug05

GCCTAGGGAAGGGACACTGATTGTTCACCATCATATCCCAGTATCTAGCACAGTGCCTAAGGGATAGGAGGGGCTCAGTAAATATCTCTTGCAAGGATGT TGTTAAATTGTAAACTCTGCCAAGGCCTTTATGTTGAATGAATGAATGAACGAATGAATGAATGCCTCTAACTCTTCATAAAATAAACACTTTTTGGAAA AAATTGCTCATGGCAGACGTCAGATGTAACCACACTGATAACTGGAAAGAAAAATGAATTTAGATGGGTTTTAGAGATAGAACAATGTTTCTAATATTCA AGCAGTCCATAAATCCCTTATACTTAATGTTAAAATTCAGGATATGATACCTAAAGAAAGGTTTTACTGTACTTGGATCAAGGCACCTACCAACTTTTTC TCTACTCATAGGCAAAGTCACTACCCTGTTTAAGGAGGTCTTTGGACGTTTCCATCTCAAGAAACAGCTCTTCAATCTCTCCCTTGTATTCCACCTTCCA GAACCACCCAGAAATTCCAGTAAAGTCTTATTGACCTGATTGTCTGTCCTCTTCGCTGAGTTCCTTTTGAATGCAGCAAATTGCTGTGCCCTTTTCTGAC TTCCATTTCACTTTTATCCAGCTTCATAGGAGCTTAGGCCCCTGCTTGTTGAGGAGGGCGTTCAGTTAGTGCTGAGACTACCTGAATAGGTCTGGTTCTA GCTACTCACAACTCAGAGAGGTAGTGAATGCTAGGAGGTAGGAAGAAGGACATTCAACCAGAATACTAAAATTGCATTTAGATAGTCCAGAAATTAGACA GTTGGCACAGCATTAGAATTCCCCATTTATCCTTACAATAGTTTGCTGAGAATGATGGTTGGGAATTGAACAATGAGAACACTTGGACACAGGAAGAGGA ACATCACACACCGGGGCCTGTTGTGGGGTGGGGAGAGCGGGGAGGGATAGCATTAGGAGATATACCTAATGTAAATGACAAGTTAATGGGTACAGCACAC CAACATGGCACGTGTATACATATGTAACAAACCTGCACGTTGTGCACATGTACCCTAGAACTTAAAGTATAATAAAAAAGTGTGTGTGTGTGTGTGTGTG TGTATATATATAAAGAATTCCCCACTTAATATTTATACATCCAAAAACTGACAAAAATAATGTCTGAACTCATGTCTGACATTCAGATTCCTCCGCCCAA AGGCAATAAATAAAATAATACTAAGATGTAAAGGCAAACCTTGTCTTCTGTCATGATCTACAGATGGCACAAATAATAGACATTTTCCCGAATAATGGCG TCTGATTTTCAAGCAATGCTTAGCCAATTGTAGATTTGGGCAGAAAAAGTTTGTACCAAGCATTTGAGCTGGAAAAGGCAGATATTTTTCTTGACCTCAC TTTTCCTAAAGTTCACAACTTCTGCTACTTAAATGAGTATCTCATCTCTAAGTGTGTGAAGTAACAGATGATGTATCTGTTTTTCATCCTCAAAGAGTTA ACTTGATAAAATAAGTACAGCTAAGGACAAACACAAACACAAAATGAGAAAACTTCATGCAGTATGTTTTGTGTTTTGTAAAGGGTGCCAATGGCATGGA GCCATCTGATTCTGTTGCAGTCTTTGGCAACACGGCCCATTTAAATTCAAGTTAGGATCTGAGTGTTTGGGTAAACAGAATAAGCTAAGAATGAGTGGCA GACATATTTCCTCAAATCCCATCTGATACAGGTTTAGATCCAGATTTTGTCATTTGACTAACTCCTTCCTTTTTTAGATGATTTGGATTTGCCTTTTTTT CTTATAATAAAAGTCATAATGCCAACCACTTTAAGAGTTCTTTTGCTGCTCTTTTTTGTTGCTATCTTTTTGTAACTAATAGGACACGTGTAAGACTATA ATACAAACAGGAGTCCTAGCATGTAATGGCTTTAGCAATTTGTGCAATATCCCTCTTTTTGTCCTTCCTGCATATTTTCTCCCTAGAAAGCAGCCAAGAA TTGGTGTTCAGAGGGTGATGCTCTGATGGTGAGCAGATCTACAGTTTGCCTAACAGTTTATTTAAGCCAAATTTATAGTTTCTGAAGCGCTAGAAGGCTG CTGTCCAAGGGGACTACTTTTTTTTTTTTTTTTAGATGGAGTCTTGCTCTGTCGCCAGGCTGGAGTGCAGAGGCGTGATCTCAGCTCACTGCAACTTCTG CCTCCCCAGTTCATGCTATTCTCCTGCTTCAGTCTCCTAAGGAGCTGGGACTACAGGCTCACACCACCACGCCCAGTTAATTTTTGTATTTTTAGTAGAG ACAGGGTTTCACCATGTTGGCCAGGATGGTCTCAATCTCTTGACCTTGTGATCCACCCGCCTCAGCCTCCCAAAGTGCTGAGATTACAGGCATGAGCCAC CACGCCCAGCCAGGGACTACTTATTAACTGGCCATTTAAAATGAAATGAAACAAGGTCCTCAATTGCTAACGATTAAGGGGAGAGGAGTGTAAATTCAGC TATTGTCTTGTCTTCAGGCAGCAGGAAATTAAGGGATTGGTAACTGTTATTTGTAATAGTAATTGATGTCACAAAAGGAGTTTTGAGAACAGGGAGTTTA GGAAAAAAATTGATCTCCTTTTATTTTTAGTCTGTTAATATTTAATGTAATATTTGATACAGTTCGATTTAGATTTACTGTTCTTTGATCTATTTTTATT TTTTGCCTTTTTTGTTGTTCTCTTTCTTCTCTCCTGCCTTCTTTTATATTGTTTAATTTTTTTTCTAAATTTCATTGTAATTTATCTATTAGCTTTTTAA CTATATCTTTTGGCATTATTTTTACAAAGTTGCTCTAGGGATTACAATATACATCCTTAAATACTCACCATCTACTTCATGGAAATTACTGTAACCTTAC AAATGTATAAATCGATTTTACCCTTGTTCATCCCATTATAATGTATATTATAAGCCTCATACAATGGTATGATTTTTACTTTTTCTCTGCGTTGTTTCTA GTTAACTATATGTGCTATCAAAGTGAGACCCCATTTATCTTTTAATCTGTTTCTGATTGTTTTTCCAGTGTCTACTTAACTGAGACTCTTACAGGTAATT TTGTAGGATACTAGGTGTTTTAGATGGCAAATATAAAGATTGTGTTTGTGTATGTGTATGTGTTTATATGTGAGGGCCAATCTATTGAAAAGAAACACTG CAGAATACTAAAGCAAAATTGAAACCATTTAGAGTTTGTAGTTGTTTTATTTTAAAAGCTAACCTTGTAAGTACTTAGTCTTTCAAAAAATAATTTTAAC TTCTATGTTTTATTTTTCTATTTTAATTTTTCCTGGGCCTTGCCTCCCAATCCTCTCACCTTCCCTTTCTTCTGTGTGACCTATAGATTCAGAAATTCCT TTCTGGGATAATTTCTGGCACTGCTTTGGAGATGGAGCCCCTGAAAATTGGCTATGGACCAAATGGATTCCCACTCTTGGGGATCTCTAGGTCATCATCA CCATCTGAACAGCTCTGAGAGAGACAAGAAGTGGAATGGAGAAGGTGATTAGATGCTCAGAGTGTGTGCACAAGGCTGGCACGCCCAGGGTCTTCCTCAT GGCACTAACAGTCTACTGAAAGGTGGAACAGAGACAAGCCTATCAACACCTACAAGACTGGTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTG TTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGG CCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAG GAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCC AGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGA ATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGG GTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACTTTAAATCCTGAAACAGTGGCAATAA ATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGA AGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAA GCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAG AAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTA ATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGA TTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAG GATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCC CACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAG AGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCT CAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATG TGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTT TTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGA AATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTC TTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGAT TTAGGAATTGCTCTTGTCATACCCCCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTG AATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTAT GGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTGGTA

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

A*GRDTDCSPSYPSI*HSA*GIGGAQ*ISLARM LLNCKLCQGLYVE*MNERMNECL*LFIK*TLFGK NCSWQTSDVTTLITGKKNEFRWVLEIEQCF*YS SSP*IPYT*C*NSGYDT*RKVLLYLDQGTYQLF LYS*AKSLPCLRRSLDVSISRNSSSISPLYSTFQ NHPEIPVKSY*PDCLSSSLSSF*MQQIAVPFSD FHFTFIQLHRSLGPCLLRRAFS*C*DYLNRSGS SYSQLREVVNARR*EEGHSTRILKLHLDSPEIRQ LAQH*NSPFILTIVC*E*WLGIEQ*EHLDTGRG TSHTGACCGVGRAGRDSIRRYT*CK*QVNGYST PTWHVYTYVTNLHVVHMYPRT*SIIKKCVCVCVC VYI*RIPHLIFIHPKTDKNNV*THV*HSDSSAQ RQ*IK*Y*DVKANLVFCHDLQMAQIIDIFPNNG V*FSSNA*PIVDLGRKSLYQAFELEKADIFLDLT FPKVHNFCYLNEYLISKCVK*QMMYLFFILKEL T**NKYS*GQTQTQNEKTSCSMFCVL*RVPMAW SHLILLQSLATRPI*IQVRI*VFG*TE*AKNEWQ TYFLKSHLIQV*IQILSFD*LLPFLDDLDLPFF LIIKVIMPTTLRVLLLLFFVAIFL*LIGHV*DY NTNRSPSM*WL*QFVQYPSFCPSCIFSP*KAAKN WCSEGDALMVSRSTVCLTVYLSQIYSF*SARRL LSKGTTFFFFFRWSLALSPGWSAEA*SQLTATS ASPVHAILLLQSPKELGLQAHTTTPS*FLYF**R QGFTMLARMVSIS*PCDPPASASQSAEITGMSH HAQPGTTY*LAI*NEMKQGPQLLTIKGRGV*IQ LLSCLQAAGN*GIGNCYL***LMSQKEF*EQGV* EKN*SPFIFSLLIFNVIFDTVRFRFTVL*SIFI FCLFCCSLSSLLPSFILFNFFSKFHCNLSISFL TISFGIIFTKLL*GLQYTSLNTHHLLHGNYCNLT NV*IDFTLVHPIIMYIISLIQWYDFYFFSALFL VNYMCYQSETPFIF*SVSDCFSSVYLTETLTGN FVGY*VF*MANIKIVFVYVYVFICEGQSIEKKHC RILKQN*NHLEFVVVLF*KLTL*VLSLSKNNFN FYVLFFYFNFSWALPPNPLTFPFFCVTYRFRNS FLG*FLALLWRWSP*KLAMDQMDSHSWGSLGHHH HLNSSERDKKWNGEGD*MLRVCAQGWHAQGLPH GTNSLLKGGTETSLSTPTRLVVLTSVARLSSKS VNAQVTDINSKGLELRKTVTTVETQNLEGLHHDG QFCHKPCPPGERKARDCTVNGDEPDCVPCQEGK EYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRT QNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKE CTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVW VKRKEVQKTCRKHRKENQGSHESPTLNPETVAI NLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNE AKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKE AYDTLIKDLKKANLCTLAEKIQTIILKDITSDS ENSNFRNEIQSLV*SEKQQIQF*VYAISV*KDS* *LAVNTAWFFTGYILSFISAEEPTYL*IFNIS* FCLQGCLKSSWENKLHQE*MQWHAKYPNRSVCR G*KIKIMLWHLTYDSVV*M*SVYVSTNVYPQANP TL*INRRSYDLLLKYQLLNRQATLPLNYL**F* RFYHISKLCL*L*EDHIYVKYMYLSAEFK*GST SKTFAQFIGVILYNISIVNSHRKH*IIMFDYYIC VCILLAQNYLLLSQASKAF*AGEYY*SFATSPF LPWCSS*WPNAPPNMEISPKNT**STKRQDCP* KF*PGLEILTALRESSFVTCHEPMFAIKDDKIDS YFSPTPENVQ*CPM*NLLQMAAYT*QW*NHHLD LGIALVIPPSF*DLRFSLLLSYV*ISLKVCIKC EF*EIIFIFL*M*TVKIVIN*SRYLEPPKELPFM EDFFAPCVWNYKI*VKVRN*IMFLV

Frame 1

PREGTLIVHHHIPVSSTVPKG*EGLSKYLLQGC C*IVNSAKAFMLNE*MNE*MNASNSS*NKHFLE KIAHGRRQM*PH**LERKMNLDGF*R*NNVSNIQ AVHKSLILNVKIQDMIPKERFYCTWIKAPTNFF STHRQSHYPV*GGLWTFPSQETALQSLPCIPPS RTTQKFQ*SLIDLIVCPLR*VPFECSKLLCPFLT SISLLSSFIGA*APAC*GGRSVSAETT*IGLVL ATHNSER**MLGGRKKDIQPEY*NCI*IVQKLD SWHSIRIPHLSLQ*FAENDGWELNNENTWTQEEE HHTPGPVVGWGERGGIALGDIPNVNDKLMGTAH QHGTCIHM*QTCTLCTCTLELKV**KSVCVCVC VYIYKEFPT*YLYIQKLTKIMSELMSDIQIPPPK GNK*NNTKM*RQTLSSVMIYRWHK**TFSRIMA SDFQAMLSQL*IWAEKVCTKHLSWKRQIFFLTS LFLKFTTSAT*MSISSLSV*SNR*CICFSSSKS* LDKISTAKDKHKHKMRKLHAVCFVFCKGCQWHG AI*FCCSLWQHGPFKFKLGSECLGKQNKLRMSG RHISSNPI*YRFRSRFCHLTNSFLF*MIWICLFF L**KS*CQPL*EFFCCSFLLLSFCN**DTCKTI IQTGVLACNGFSNLCNIPLFVLPAYFLPRKQPR IGVQRVML*W*ADLQFA*QFI*AKFIVSEALEGC CPRGLLFFFFLDGVLLCRQAGVQRRDLSSLQLL PPQFMLFSCFSLLRSWDYRLTPPRPVNFCIFSR DRVSPCWPGWSQSLDLVIHPPQPPKVLRLQA*AT TPSQGLLINWPFKMK*NKVLNC*RLRGEECKFS YCLVFRQQEIKGLVTVICNSN*CHKRSFENREF RKKIDLLLFLVC*YLM*YLIQFDLDLLFFDLFLF FAFFVVLFLLSCLLLYCLIFFLNFIVIYLLAF* LYLLALFLQSCSRDYNIHP*ILTIYFMEITVTL QMYKSILPLFIPL*CIL*ASYNGMIFTFSLRCF* LTICAIKVRPHLSFNLFLIVFPVST*LRLLQVI L*DTRCFRWQI*RLCLCMCMCLYVRANLLKRNT AEY*SKIETI*SL*LFYFKS*PCKYLVFQKIILT SMFYFSILIFPGPCLPILSPSLSSV*PIDSEIP FWDNFWHCFGDGAPENWLWTKWIPTLGDL*VII TI*TALRETRSGMEKVIRCSECVHKAGTPRVFLM ALTVY*KVEQRQAYQHLQDWWFLRLLLDYRPKV LMPK*LTSTPRDWN*GRLLLQLRLRTWKACIMM ANSAISPVLQVKGKLGTAQSMGMNQTACPAKKGR STQTKPIFLPNAEDVDCVMKDMA*KWK*TAPGP RIPSADVNQTFFVTLLYVNTVTLAPNVNMESSR NAHSPATPSAKRKDPDLTWGGFVFFFCQFH*LFG *RERKYRKHAESTERKTKVLMNLQL*ILKQWQ* IYLMLT*VNISPLLLES*H*VKLKALFERMVSM KPK*MRSRMTMSKTQQNRKFNCFVIGINFMERKK RMTH*LKISKKPIFVLLQRKFRLSSSRTLLVTQ KIQTSEMKSKAWSRVKNNKFSSEYMQLVFEKIL NSWL*ILLGFLLGTFYHLLALKSQHICRFLISHD SASKDV*NLVGKTNFIKSKCSGMLSTQIGVYAE DERLRLCSGI*HMIL*YECNQCMLVQMSIHRLT PLYESIEEAMTFC*NISY*TGRPLCL*ITSDNSR DFTIFLNFVYNSEKIIFM*SICI*VQNLNKALP QRPLHSLLVSYYTIFQL*IHIENIKL*CLTIIY VYAFYWLKTTYFFLRHQKHFEQESITRALPPLHF CLGAHLNGLMHPQTWKYHQKILNSPPKGKTALR NSSLVWRY*LLSEKVAL*HVMNPCLQSKMIK*I LIFPPPPKMFNNVPCKTCYKWQLIHSNGKIIIWI *ELLLSYPQVSKI*DSPYYYPTFKYL*KFVLNV NFKK*YLYFCKCKL*R*L*TEADTWNHLKNFHL WRIFLPLVFGIIKYR*KYVIK*CFW

Frame 2

LGKGH*LFTIISQYLAQCLRDRRGSVNISCKDV VKL*TLPRPLC*MNE*TNE*MPLTLHKINTFWK KLLMADVRCNHTDNWKEK*I*MGFRDRTMFLIF KQSINPLYLMLKFRI*YLKKGFTVLGSRHLPTFS LLIGKVTTLFKEVFGRFHLKKQLFNLSLVFHLP EPPRNSSKVLLT*LSVLFAEFLLNAANCCALF* LPFHFYPAS*ELRPLLVEEGVQLVLRLPE*VWF* LLTTQRGSEC*EVGRRTFNQNTKIAFR*SRN*T VGTALEFPIYPYNSLLRMMVGN*TMRTLGHRKR NITHRGLLWGGESGEG*H*EIYLM*MTS*WVQHT NMARVYICNKPARCAHVP*NLKYNKKVCVCVCV CIYIKNSPLNIYTSKN*QK*CLNSCLTFRFLRP KAINKIILRCKGKPCLLS*STDGTNNRHFPE*WR LIFKQCLANCRFGQKKFVPSI*AGKGRYFS*PH FS*SSQLLLLK*VSHL*VCEVTDDVSVFHPQRV NLIK*VQLRTNTNTK*ENFMQYVLCFVKGANGME PSDSVAVFGNTAHLNSS*DLSVWVNRIS*E*VA DIFPQIPSDTGLDPDFVI*LTPSFFR*FGFAFF SYNKSHNANHFKSSFAALFCCYLFVTNRTRVRL* YKQES*HVMALAICAISLFLSFLHIFSLESSQE LVFRG*CSDGEQIYSLPNSLFKPNL*FLKR*KA AVQGDYFFFFF*MESCSVARLECRGVISAHCNFC LPSSCYSPASVS*GAGTTGSHHHAQLIFVFLVE TGFHHVGQDGLNLLTL*STRLSLPKC*DYRHEP PRPARDYLLTGHLK*NETRSSIAND*GERSVNSA IVLSSGSRKLRDW*LLFVIVIDVTKGVLRTGSL GKKLISFYF*SVNI*CNI*YSSI*IYCSLIYFY FLPFLLFSFFSPAFFYIV*FFF*ISL*FIY*LFN YIFWHYFYKVALGITIYILKYSPSTSWKLL*PY KCINRFYPCSSHYNVYYKPHTMV*FLLFLCVVS S*LYVLSK*DPIYLLICF*LFFQCLLN*DSYR*F CRILGVLDGKYKDCVCVCVCVYM*GPIY*KETL QNTKAKLKPFRVCSCFILKANLVST*SFKK*F* LLCFIFLF*FFLGLASQSSHLPFLLCDL*IQKFL SGIISGTALEMEPLKIGYGPNGFPLLGISRSSS PSEQL*ERQEVEWRR*LDAQSVCTRLARPGSSS WH*QSTERWNRDKPINTYKTGGSYVCC*IIVQKC *CPSD*HQLQGIGIEEDCYYS*DSELGRPAS*W PILP*ALSSR*KES*GLHSQWG*TRLRALPRRE GVHRQSPFFFQMQKM*IV**RTWLRSGNKLHPDP EYQVQM*TKLFL*LYCM*TL*PLHQM*TWNHQG MHTHQQHQVQRGRIQI*LGVALSSSFANSTNCL GEEKGSTENMQKAQKGKPRFS*ISNFKS*NSGNK FI*C*LE*IYHHYCWSHDTKSS*RLCSKEWCQ* SQNR*DQE*QCPRHSRTESSTAS*LASTSWKER SV*HID*RSQKSQSLYSCRENSDYHPQGHY**LR KFKLQK*NPKLGLE*KTTNSVLSICN*CLKRFL IAGCKYCLVFYWVHFIIY*R*RANIFVDF*YLM ILPPRMFKI*LGKQTSSRVNAVAC*VPK*ECMQR MKD*DYALASNI*FCSMNVISVC*YKCLSTG*P HSMNQ*KKL*PFAEISVTEQAGHFASKLPLIIL EILPYF*TLFITLRRSYLCKVYVFECRI*IRLYL KDLCTVYWCHIIQYFNCEFT*KTLNYNV*LLYM CMHFTGSKLPTSFSGIKSILSRRVLLELCHLSI FALVLILMA*CTPKHGNITKKYLIVHQKARLPLE ILAWFGDTNCSQRK*LCDMS*THVCNQR**NRF LFFPHPRKCSIMSHVKPATNGSLYIAMVKSSSG FRNCSCHTPKFLRFKILLTTILRLNIFESLY*M* ILRNNIYISVNVNCEDSYKLKQIPGTT*RTSIY GGFFCPLCLEL*NIGKST*LNNVFG

Sequence FAS.dAug05

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

Frame 1

Frame 2

Sequence FAS.eAug05

GGCAGTTCTCAGACGTAGGAAATAAGTCAGCACCGAAGCAGTGGTTAAGCCGGAGGGCTCGGAAGAACGGCACCTTTTCTTTCTCGAAAAAGTTATATGG GGGCTGAATGAGCTTCTGGAGGCTTGTTTACCGTTTTTTATTGTCACACAGAAAAAGAAACTGCCTTGTCTCCCTTCCGGGAATTCTCTCTTTAAGACTG TTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGT TGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGG GATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGAC ATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGA CCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTT TGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATG AATCTCCAACTTTAAATCCTATGGAGTCTTGCTCCATAGCCCAG

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

GSSQT*EISQHRSSG*AGGLGRTAPFLSRKSYM GAE*ASGGLFTVFYCHTEKETALSPFREFSL*DC SYVCC*IIVQKC*CPSD*HQLQGIGIEEDCYYS *DSELGRPAS*WPILP*ALSSR*KES*GLHSQW G*TRLRALPRREGVHRQSPFFFQMQKM*IV**RT WLRSGNKLHPDPEYQVQM*TKLFL*LYCM*TL* PLHQM*TWNHQGMHTHQQHQVQRGRIQI*LGVA LSSSFANSTNCLGEEKGSTENMQKAQKGKPRFS* ISNFKSYGVLLHSP

Frame 1

AVLRRRK*VSTEAVVKPEGSEERHLFFLEKVIW GLNELLEACLPFFIVTQKKKLPCLPSGNSLFKT VLTSVARLSSKSVNAQVTDINSKGLELRKTVTTV ETQNLEGLHHDGQFCHKPCPPGERKARDCTVNG DEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEG HGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCD PCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWL CLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSH ESPTLNPMESCSIAQ

Frame 2

QFSDVGNKSAPKQWLSRRARKNGTFSFSKKLYG G*MSFWRLVYRFLLSHRKRNCLVSLPGILSLRL FLRLLLDYRPKVLMPK*LTSTPRDWN*GRLLLQ LRLRTWKACIMMANSAISPVLQVKGKLGTAQSMG MNQTACPAKKGRSTQTKPIFLPNAEDVDCVMKD MA*KWK*TAPGPRIPSADVNQTFFVTLLYVNTV TLAPNVNMESSRNAHSPATPSAKRKDPDLTWGGF VFFFCQFH*LFG*RERKYRKHAESTERKTKVLM NLQL*ILWSLAP*P

Sequence FAS.fAug05

CCTACCCGCGCGCAGGCCAAGTTGCTGAATCAATGGAGCCCTCCCCAACCCGGGCGTTCCCCAGCGAGGCTTCCTTCCCATCCTCCTGACCACCGGGGCT TTTCGTGAGCTCGTCTCTGATCTCGCGCAAGAGTGACACACAGGTGTTCAAAGACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGA GCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGT ACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTA TCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCC TGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTG CCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAAC TGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATG GAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCC ACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACTTTAAATCCTATG TTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGA TGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACA TTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACT TCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGT AAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAA GGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTA AGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAAT CAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCAT ATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTG CACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTT ACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGC TCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTG GTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCC CACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCT CTTGTCATACCCCCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAA TAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTT TGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTGGTA

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

PTRAQAKLLNQWSPPQPGRSPARLPSHPPDHRG FS*ARL*SRARVTHRCSKTLLGSEGSGLRVTWLE PQGRALARNTP*GQPWLPRRSCLFSRGLVDPLS TELGKLFHFGGLLNNHAGHLDPPTSGSYVCC*I IVQKC*CPSD*HQLQGIGIEEDCYYS*DSELGRP AS*WPILP*ALSSR*KES*GLHSQWG*TRLRAL PRREGVHRQSPFFFQMQKM*IV**RTWLRSGNK LHPDPEYQVQM*TKLFL*LYCM*TL*PLHQM*TW NHQGMHTHQQHQVQRGRIQI*LGVALSSSFANS TNCLGEEKGSTENMQKAQKGKPRFS*ISNFKSY VDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKID EIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDT LIKDLKKANLCTLAEKIQTIILKDITSDSENSN FRNEIQSLV*SEKQQIQF*VYAISV*KDS**LAV NTAWFFTGYILSFISAEEPTYL*IFNIS*FCLQ GCLKSSWENKLHQE*MQWHAKYPNRSVCRG*KI KIMLWHLTYDSVV*M*SVYVSTNVYPQANPTL*I NRRSYDLLLKYQLLNRQATLPLNYL**F*RFYH ISKLCL*L*EDHIYVKYMYLSAEFK*GSTSKTF AQFIGVILYNISIVNSHRKH*IIMFDYYICVCIL LAQNYLLLSQASKAF*AGEYY*SFATSPFLPWC SS*WPNAPPNMEISPKNT**STKRQDCP*KF*P GLEILTALRESSFVTCHEPMFAIKDDKIDSYFSP TPENVQ*CPM*NLLQMAAYT*QW*NHHLDLGIA LVIPPSF*DLRFSLLLSYV*ISLKVCIKCEF*E IIFIFL*M*TVKIVIN*SRYLEPPKELPFMEDFF APCVWNYKI*VKVRN*IMFLV

Frame 1

LPARRPSC*INGALPNPGVPQRGFLPILLTTGA FRELVSDLAQE*HTGVQRRFWGVREAVYE*LGW SLRGGHWHGTHPEASPGCPGGAASSPAGWWTRSV RSWGSSFTSEDCSTTMLGIWTLLPLVLTSVARL SSKSVNAQVTDINSKGLELRKTVTTVETQNLEG LHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPC QEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEIN CTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEH GIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIP LIVWVKRKEVQKTCRKHRKENQGSHESPTLNPM LT*VNISPLLLES*H*VKLKALFERMVSMKPK* MRSRMTMSKTQQNRKFNCFVIGINFMERKKRMTH *LKISKKPIFVLLQRKFRLSSSRTLLVTQKIQT SEMKSKAWSRVKNNKFSSEYMQLVFEKILNSWL *ILLGFLLGTFYHLLALKSQHICRFLISHDSASK DV*NLVGKTNFIKSKCSGMLSTQIGVYAEDERL RLCSGI*HMIL*YECNQCMLVQMSIHRLTPLYE SIEEAMTFC*NISY*TGRPLCL*ITSDNSRDFTI FLNFVYNSEKIIFM*SICI*VQNLNKALPQRPL HSLLVSYYTIFQL*IHIENIKL*CLTIIYVYAF YWLKTTYFFLRHQKHFEQESITRALPPLHFCLGA HLNGLMHPQTWKYHQKILNSPPKGKTALRNSSL VWRY*LLSEKVAL*HVMNPCLQSKMIK*ILIFP PPPKMFNNVPCKTCYKWQLIHSNGKIIIWI*ELL LSYPQVSKI*DSPYYYPTFKYL*KFVLNVNFKK *YLYFCKCKL*R*L*TEADTWNHLKNFHLWRIF LPLVFGIIKYR*KYVIK*CFW

Frame 2

YPRAGQVAESMEPSPTRAFPSEASFPSS*PPGL FVSSSLISRKSDTQVFKDASGE*GKRFTSDLAG ASGAGTGTEHTLRPALAAQAELPLLPRVGGPAQ YGVGEALSLRRIAQQPCWASGPSYLWFLRLLLDY RPKVLMPK*LTSTPRDWN*GRLLLQLRLRTWKA CIMMANSAISPVLQVKGKLGTAQSMGMNQTACP AKKGRSTQTKPIFLPNAEDVDCVMKDMA*KWK*T APGPRIPSADVNQTFFVTLLYVNTVTLAPNVNM ESSRNAHSPATPSAKRKDPDLTWGGFVFFFCQF H*LFG*RERKYRKHAESTERKTKVLMNLQL*ILC *LE*IYHHYCWSHDTKSS*RLCSKEWCQ*SQNR *DQE*QCPRHSRTESSTAS*LASTSWKERSV*H ID*RSQKSQSLYSCRENSDYHPQGHY**LRKFKL QK*NPKLGLE*KTTNSVLSICN*CLKRFLIAGC KYCLVFYWVHFIIY*R*RANIFVDF*YLMILPP RMFKI*LGKQTSSRVNAVAC*VPK*ECMQRMKD* DYALASNI*FCSMNVISVC*YKCLSTG*PHSMN Q*KKL*PFAEISVTEQAGHFASKLPLIILEILP YF*TLFITLRRSYLCKVYVFECRI*IRLYLKDLC TVYWCHIIQYFNCEFT*KTLNYNV*LLYMCMHF TGSKLPTSFSGIKSILSRRVLLELCHLSIFALV LILMA*CTPKHGNITKKYLIVHQKARLPLEILAW FGDTNCSQRK*LCDMS*THVCNQR**NRFLFFP HPRKCSIMSHVKPATNGSLYIAMVKSSSGFRNC SCHTPKFLRFKILLTTILRLNIFESLY*M*ILRN NIYISVNVNCEDSYKLKQIPGTT*RTSIYGGFF CPLCLEL*NIGKST*LNNVFG

Sequence FAS.gAug05

CCGGGGATAGGCAAAGTGGGGCGGGCGCGGGACGCGTGCGGGATTGCGGCGGCAGCGGCGCACGCGGGCACCTGGGAGCGGCGGGCTGCTGCGGGAGGCG TTGGAGACTGGCTCCCGGGGGCTGTTAGGACCTTCCCTCAGGCCCGGGTGCTCAGAACGCTGGAGGACTTGCTTTTCTTGGGCCTTGATGCGAAGTGCTG ACCCCGCTGGGCAGGCGGGGCAGCTCCGGCGCTCCTCGGAGACCACTGCGCTCCACGTTGAGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGT TAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGC CAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGG AGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCA GAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAA TGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGG

113302 (forward primer)
TNFRSF6-6 (reverse primer)
Multiple exon skipping

Frame 0

PGIGKVGRARDACGIAAAAAHAGTWERRAAAGG VGDWLPGAVRTFPQARVLRTLEDLLFLGLDAKC* PRWAGGAAPALLGDHCAPR*GSYVCC*IIVQKC *CPSD*HQLQGIGIEEDCYYS*DSELGRPAS*W PILP*ALSSR*KES*GLHSQWG*TRLRALPRREG VHRQSPFFFQMQKM*IV**RTWLRSGNKLHPDP EYQVQM*TKLFL*LYCM*TL*PLHQM*TWNHQG MHTHQQHQVQRGRIQI*LGVALSSSFANSTNCL

Frame 1

RG*AKWGGRGTRAGLRRQRRTRAPGSGGLLREA LETGSRGLLGPSLRPGCSERWRTCFSWALMRSA DPAGQAGQLRRSSETTALHVEVLTSVARLSSKSV NAQVTDINSKGLELRKTVTTVETQNLEGLHHDG QFCHKPCPPGERKARDCTVNGDEPDCVPCQEGK EYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQ NTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKE CTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVW

Frame 2

GDRQSGAGAGRVRDCGGSGARGHLGAAGCCGRR WRLAPGGC*DLPSGPGAQNAGGLAFLGP*CEVL TPLGRRGSSGAPRRPLRSTLRFLRLLLDYRPKV LMPK*LTSTPRDWN*GRLLLQLRLRTWKACIMMA NSAISPVLQVKGKLGTAQSMGMNQTACPAKKGR STQTKPIFLPNAEDVDCVMKDMA*KWK*TAPGP RIPSADVNQTFFVTLLYVNTVTLAPNVNMESSRN AHSPATPSAKRKDPDLTWGGFVFFFCQFH*LFG