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Sequences

Sequence SDCCAG8.bAug05

GTGCGGGATTCTAGGCTCCCCTGTGACAGCCGCGGCAGGAAGCAGGCGGGCGCTCCCCGGCCACAGGCCTGTTGTTCTCGGAAGGGAGAAAGCTGGACAT TTCCCCACGTAACTCCCAGCTCTGGGCCTAGAGTGCGTGCATGGCGAAGTCCCCGGAGAACTCTACCCTGGAGGAGATTCTGGGGCAGTATCAACGGAGT CTCCGGGGTTGTTCTTGCAGAACATGCCAGCAGAAGCATTCACCAACTGACATGTGCCCTGAAAGAAGGCGATGTCACTATTGGAGAAGATGCACCAAAT CTTTCTTTTAGCACCAGTGTGGGAAATGAGGACGCCAGGACAGCCTGGCCCGAATTACAACAGAGCCATGCTGTTAATCAGCTCAAAGATTTGTTGCGCC AACAAGCAGATAAGGAAAGTGAAGTATCTCCGTCAAGAAGAAGAAAAATGTCCCCCTTGAGGTCATTAGAACATGAGGAAACCAATATGCCTACTATGCA CGACCTTGTTCATACTATTAATGACCAGTCTCAATATATTCATCATTTAGAGGCAGAAGTTAAGTTCTGCAAGGAGGAACTCTCTGGAATGAAAAATAAA ATACAAGTAGTTGTGCTTGAAAACGAAGGGCTCCAGCAACAGCTAAAATCTCAAAGACAAGAGGAGACACTGAGGGAACAAACACTTCTGGATGCATCCG AGAAGCTAAAACTTACTTATGAGGAAAAGTGTGAAATTGAGGAATCCCAATTGAAGTTTTTGAGGAACGACTTAGCTGAATATCAGAGAACTTGTGAAGA TCTTAAAGAGCAACTAAAGCATAAAGAATTTCTTCTGGCTGCTAATACTTGTAACCGTGTTGGTGGTCTTTGTTTGAAATGTGCTCAGCATGAAGCTGTT CTTTCCCAAACCCATACTAATGTTCATATGCAGACCATCGAAAGACTGGTTAAAGAAAGAGATGACTTGATGTCTGCACTAGTTTCCGTAAGGAGCAGCT TGGCAGATACGCAGCAAAGAGAAGCAAGTGCTTATGAACAGGTGAAACAAGTTTTGCAAATATCTGAGGAAGCCAATTTTGAAAAAACCAAGGCTTTAAT CCAGTGTGACCAGTTGAGGAAGGAGCTGGAGAGGCAGGCGGAGCGACTTGAAAAAGAACTTGCATCTCAGCAAGAGAAAAGGGCCATTGAGAAAGACATG ATGAAAAAGGAAATAACGAAAGAAAGGGAGTACATGGGATCAAAGATGTTGATCTTGTCTCAGAATATTGCCCAACTGGAGGCCCAGGTGGAAAAGGTTA CAAAGGAAAAGATTTCAGCTATTAATCAACTGGAGGAAATTCAAAGCCAGCTGGCTTCTCGGGAAATGGATGTCACAAAGGTGTGTGGAGAAATGCGCTA TCAGCTGAATAAAACCAACATGGAGAAGGATGAGGCAGAAAAGGAGCACAGAGAGTTCAGAGCAAAAACTAACAGGGATCTTGAAATTAAAGATCAGGAA ATAGAGAAATTGAGAATAGAACTGGATGAAAGCAAACAACACTTGGAACAGGAGCAGCAGAAGGCAGCCCTGGCCAGAGAGGAGTGCCTGAGACTAACAG AACTGCTGGGCGAATCTGAGCACCAACTGCACCTCACCAGATCTGAAATAGCTCAACTCAGTCAAGAAAAAAGGTATACATATGATAAATTGGGAAAGTT ACAGAGAAGAAATGAAGAATTGGAGGAACAGTGTGTCCAGCATGGGAGAGTACATGAGACGATGAAGCAAAGGCTAAGGCAGCTGGATAAGCACAGCCAG GCCACAGCCCAGCAGCTGGTGCAGCTCCTCAGCAAGCAGAACCAGCTTCTCCTGGAGAGGCAGAGCCTGTCGGAAGAGGTGGACCGGCTGCGGACCCAGT TACCCAGCATGCCACAATCTGATTGCTGACCTGGATGGAACAGAGTGAAATAAATGATTTACAAAGAGATATTTACATTCATCTGGTTTAGACTTAATAT GCCACAACGCACCACGACCTTCCCAGGGTGACACCGCCTCAGCCTGCAGTGGGGCTGGTCCTCATCAACGCGGGCGCTGTCCCCGCACGCAGTCGGGCTG GAGCTGGAGTCTGACTCTAGCTGAGCAGAGCTCCTGGTGTATGTTTTCAGAAATGGCTTGAAGTTATGTGTTTAAATCTGCTCATTCGTATGCTAGGTTA TACATATGATTTTCAATAAATGAACTTTTTAAAGACTTGAGTTGTAATGTTTCCTTTTTATCTTGTAGTGATGAACAAAACACACCAAAAAGGCACATAT TTTAACTAGGCCAAAGTATATTAAGGACCAAACTTTTTTTCCTGCCATTCATTTTTCTTTTCATGATCTATATATCAATCATCCTTCA

SDCCAG8-F1 (forward primer)
SDCCAG8-R1 (reverse primer)
Multiple exon skipping

Frame 0

VRDSRLPCDSRGRKQAGAPRPQACCSRKGESWT FPHVTPSSGPRVRAWRSPRRTLPWRRFWGSINGV SGVVLAEHASRSIHQLTCALKEGDVTIGEDAPN LSFSTSVGNEDARTAWPELQQSHAVNQLKDLLR QQADKESEVSPSRRRKMSPLRSLEHEETNMPTMH DLVHTINDQSQYIHHLEAEVKFCKEELSGMKNK IQVVVLENEGLQQQLKSQRQEETLREQTLLDAS EKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCED LKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAV LSQTHTNVHMQTIERLVKERDDLMSALVSVRSS LADTQQREASAYEQVKQVLQISEEANFEKTKALI QCDQLRKELERQAERLEKELASQQEKRAIEKDM MKKEITKEREYMGSKMLILSQNIAQLEAQVEKV TKEKISAINQLEEIQSQLASREMDVTKVCGEMRY QLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQE IEKLRIELDESKQHLEQEQQKAALAREECLRLT ELLGESEHQLHLTRSEIAQLSQEKRYTYDKLGKL QRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQ ATAQQLVQLLSKQNQLLLERQSLSEEVDRLRTQ LPSMPQSDC*PGWNRVK*MIYKEIFTFIWFRLNM PQRTTTFPG*HRLSLQWGWSSSTRALSPHAVGL ELESDSS*AELLVYVFRNGLKLCV*ICSFVC*V IHMIFNK*TF*RLEL*CFLFIL***TKHTKKAHI LTRPKYIKDQTFFPAIHFSFHDLYINHPS

Frame 1

CGILGSPVTAAAGSRRALPGHRPVVLGREKAGH FPT*LPALGLECVHGEVPGELYPGGDSGAVSTE SPGLFLQNMPAEAFTN*HVP*KKAMSLLEKMHQI FLLAPVWEMRTPGQPGPNYNRAMLLISSKICCA NKQIRKVKYLRQEEEKCPP*GH*NMRKPICLLC TTLFILLMTSLNIFII*RQKLSSARRNSLE*KIK YK*LCLKTKGSSNS*NLKDKRRH*GNKHFWMHP RS*NLLMRKSVKLRNPN*SF*GTT*LNIRELVK ILKSN*SIKNFFWLLILVTVLVVFV*NVLSMKLF FPKPILMFICRPSKDWLKKEMT*CLH*FP*GAA WQIRSKEKQVLMNR*NKFCKYLRKPILKKPRL* SSVTS*GRSWRGRRSDLKKNLHLSKRKGPLRKT* *KRK*RKKGSTWDQRC*SCLRILPNWRPRWKRL QRKRFQLLINWRKFKASWLLGKWMSQRCVEKCA IS*IKPTWRRMRQKRSTESSEQKLTGILKLKIRK *RN*E*NWMKANNTWNRSSRRQPWPERSA*D*Q NCWANLSTNCTSPDLK*LNSVKKKGIHMINWES YREEMKNWRNSVSSMGEYMRR*SKG*GSWISTAR PQPSSWCSSSASRTSFSWRGRACRKRWTGCGPS YPACHNLIADLDGTE*NK*FTKRYLHSSGLDLI CHNAPRPSQGDTASACSGAGPHQRGRCPRTQSGW SWSLTLAEQSSWCMFSEMA*SYVFKSAHSYARL YI*FSINELFKDLSCNVSFLSCSDEQNTPKRHI F*LGQSILRTKLFFLPFIFLFMIYISIIL

Frame 2

AGF*APL*QPRQEAGGRSPATGLLFSEGRKLDI SPRNSQLWA*SACMAKSPENSTLEEILGQYQRS LRGCSCRTCQQKHSPTDMCPERRRCHYWRRCTK SFF*HQCGK*GRQDSLARITTEPCC*SAQRFVAP TSR*GK*SISVKKKKNVPLEVIRT*GNQYAYYA RPCSYY**PVSIYSSFRGRS*VLQGGTLWNEK* NTSSCA*KRRAPATAKISKTRGDTEGTNTSGCIR EAKTYL*GKV*N*GIPIEVFEERLS*ISENL*R S*RATKA*RISSGC*YL*PCWWSLFEMCSA*SC SFPNPY*CSYADHRKTG*RKR*LDVCTSFRKEQL GRYAAKRSKCL*TGETSFANI*GSQF*KNQGFN PV*PVEEGAGEAGGAT*KRTCISAREKGH*ERH DEKGNNERKGVHGIKDVDLVSEYCPTGGPGGKGY KGKDFSY*STGGNSKPAGFSGNGCHKGVWRNAL SAE*NQHGEG*GRKGAQRVQSKN*QGS*N*RSG NREIENRTG*KQTTLGTGAAEGSPGQRGVPETNR TAGRI*APTAPHQI*NSSTQSRKKVYI**IGKV TEKK*RIGGTVCPAWEST*DDEAKAKAAG*AQP GHSPAAGAAPQQAEPASPGEAEPVGRGGPAADPV TQHATI*LLTWMEQSEINDLQRDIYIHLV*T*Y ATTHHDLPRVTPPQPAVGLVLINAGAVPARSRA GAGV*L*LSRAPGVCFQKWLEVMCLNLLIRMLGY TYDFQ*MNFLKT*VVMFPFYLVVMNKTHQKGTY FN*AKVY*GPNFFSCHSFFFS*SIYQSSF

Sequence SDCCAG8.aAug05

GTGCGGGATTCTAGGCTCCCCTGTGACAGCCGCGGCAGGAAGCAGGCGGGCGCTCCCCGGCCACAGGCCTGTTGTTCTCGGAAGGGAGAAAGCTGGACAT TTCCCCACGTAACTCCCAGCTCTGGGCCTAGAGTGCGTGCATGGCGAAGTCCCCGGAGAACTCTACCCTGGAGGAGATTCTGGGGCAGTATCAACGGAGT CTCCGGGAACATGCCAGCAGAAGCATTCACCAACTGACATGTGCCCTGAAAGAAGGCGATGTCACTATTGGAGAAGATGCACCAAATCTTTCTTTTAGCA CCAGTGTGGGAAATGAGGACGCCAGGACAGCCTGGCCCGAATTACAACAGAGCCATGCTGTTAATCAGCTCAAAGATTTGTTGCGCCAACAAGCAGATAA GGAAAGTGAAGTATCTCCGTCAAGAAGAAGAAAAATGTCCCCCTTGAGGTCATTAGAACATGAGGAAACCAATATGCCTACTATGCACGACCTTGTTCAT ACTATTAATGACCAGTCTCAATATATTCATCATTTAGAGGCAGAAGTTAAGTTCTGCAAGGAGGAACTCTCTGGAATGAAAAATAAAATACAAGTAGTTG TGCTTGAAAACGAAGGGCTCCAGCAACAGCTAAAATCTCAAAGACAAGAGGAGACACTGAGGGAACAAACACTTCTGGATGCATCCGGAAACATGCACAA TTCTTGGATTACAACAGGTGAAGATTCTGGGGTGGGCGAAACCTCCAAAAGACCATTTTCCCATGACAATGCAGATTTTGGCAAAGCTGCATCTGCTGGT GAGCAGCTAGAACTGGAGAAGCTAAAACTTACTTATGAGGAAAAGTGTGAAATTGAGGAATCCCAATTGAAGTTTTTGAGGAACGACTTAGCTGAATATC AGAGAACTTGTGAAGATCTTAAAGAGCAACTAAAGCATAAAGAATTTCTTCTGGCTGCTAATACTTGTAACCGTGTTGGTGGTCTTTGTTTGAAATGTGC TCAGCATGAAGCTGTTCTTTCCCAAACCCATACTAATGTTCATATGCAGACCATCGAAAGACTGGTTAAAGAAAGAGATGACTTGATGTCTGCACTAGTT TCCGTAAGGAGCAGCTTGGCAGATACGCAGCAAAGAGAAGCAAGTGCTTATGAACAGGTGAAACAAGTTTTGCAAATATCTGAGGAAGCCAATTTTGAAA AAACCAAGGCTTTAATCCAGTGTGACCAGTTGAGGAAGGAGCTGGAGAGGCAGGCGGAGCGACTTGAAAAAGAACTTGCATCTCAGCAAGAGAAAAGGGC CATTGAGAAAGACATGATGAAAAAGGAAATAACGAAAGAAAGGGAGTACATGGGATCAAAGATGTTGATCTTGTCTCAGAATATTGCCCAACTGGAGGCC CAGGTGGAAAAGGTTACAAAGGAAAAGATTTCAGCTATTAATCAACTGGAGGAAATTCAAAGCCAGCTGGCTTCTCGGGAAATGGATGTCACAAAGGTGT GTGGAGAAATGCGCTATCAGCTGAATAAAACCAACATGGAGAAGGATGAGGCAGAAAAGGAGCACAGAGAGTTCAGAGCAAAAACTAACAGGGATCTTGA AATTAAAGATCAGGAAATAGAGAAATTGAGAATAGAACTGGATGAAAGCAAACAACACTTGGAACAGGAGCAGCAGAAGGCAGCCCTGGCCAGAGAGGAG TGCCTGAGACTAACAGAACTGCTGGGCGAATCTGAGCACCAACTGCACCTCACCAGACAGGAAAAAGATAGCATTCAGCAGAGCTTTAGCAAGGAAGCAA AGGCCCAAGCCCTTCAGGCCCAGCAAAGAGAGCAGGAGCTGACACAGAAGATACAGCAAATGGAGGCCCAGCATGACAAAACTGAAAATGAACAGTATTT GTTGCTGACCTCCCAGAATACATTTTTGACAAAGTTAAAGGAAGAATGCTGTACATTAGCCAAGAAACTGGAACAAATCTCTCAAAAAACCAGATCTGAA ATAGCTCAACTCAGTCAAGAAAAAAGGTATACATATGATAAATTGGGAAAGTTACAGAGAAGAAATGAAGAATTGGAGGAACAGTGTGTCCAGCATGGGA GAGTACATGAGACGATGAAGCAAAGGCTAAGGCAGCTGGATAAGCACAGCCAGGCCACAGCCCAGCAGCTGGTGCAGCTCCTCAGCAAGCAGAACCAGCT TCTCCTGGAGAGGCAGAGCCTGTCGGAAGAGGTGGACCGGCTGCGGACCCAGTTACCCAGCATGCCACAATCTGATTGCTGACCTGGATGGAACAGAGTG AAATAAATGATTTACAAAGAGATATTTACATTCATCTGGTTTAGACTTAATATGCCACAACGCACCACGACCTTCCCAGGGTGACACCGCCTCAGCCTGC AGTGGGGCTGGTCCTCATCAACGCGGGCGCTGTCCCCGCACGCAGTCGGGCTGGAGCTGGAGTCTGACTCTAGCTGAGCAGAGCTCCTGGTGTATGTTTT CAGAAATGGCTTGAAGTTATGTGTTTAAATCTGCTCATTCGTATGCTAGGTTATACATATGATTTTCAATAAATGAACTTTTTAAAGACTTGAGTTGTAA TGTTTCCTTTTTATCTTGTAGTGATGAACAAAACACACCAAAAAGGCACATATTTTAACTAGGCCAAAGTATATTAAGGACCAAACTTTTTTTCCTGCCA TTCATTTTTCTTTTCATGATCTATATATCAATCATCCTTCA

SDCCAG8-F1 (forward primer)
SDCCAG8-R1 (reverse primer)
Multiple exon skipping

Frame 0

VRDSRLPCDSRGRKQAGAPRPQACCSRKGESWT FPHVTPSSGPRVRAWRSPRRTLPWRRFWGSINGV SGNMPAEAFTN*HVP*KKAMSLLEKMHQIFLLA PVWEMRTPGQPGPNYNRAMLLISSKICCANKQI RKVKYLRQEEEKCPP*GH*NMRKPICLLCTTLFI LLMTSLNIFII*RQKLSSARRNSLE*KIKYK*L CLKTKGSSNS*NLKDKRRH*GNKHFWMHPETCT ILGLQQVKILGWAKPPKDHFPMTMQILAKLHLLV SS*NWRS*NLLMRKSVKLRNPN*SF*GTT*LNI RELVKILKSN*SIKNFFWLLILVTVLVVFV*NV LSMKLFFPKPILMFICRPSKDWLKKEMT*CLH*F P*GAAWQIRSKEKQVLMNR*NKFCKYLRKPILK KPRL*SSVTS*GRSWRGRRSDLKKNLHLSKRKG PLRKT**KRK*RKKGSTWDQRC*SCLRILPNWRP RWKRLQRKRFQLLINWRKFKASWLLGKWMSQRC VEKCAIS*IKPTWRRMRQKRSTESSEQKLTGIL KLKIRK*RN*E*NWMKANNTWNRSSRRQPWPERS A*D*QNCWANLSTNCTSPDRKKIAFSRALARKQ RPKPFRPSKESRS*HRRYSKWRPSMTKLKMNSI CC*PPRIHF*QS*RKNAVH*PRNWNKSLKKPDLK *LNSVKKKGIHMINWESYREEMKNWRNSVSSMG EYMRR*SKG*GSWISTARPQPSSWCSSSASRTS FSWRGRACRKRWTGCGPSYPACHNLIADLDGTE* NK*FTKRYLHSSGLDLICHNAPRPSQGDTASAC SGAGPHQRGRCPRTQSGWSWSLTLAEQSSWCMF SEMA*SYVFKSAHSYARLYI*FSINELFKDLSCN VSFLSCSDEQNTPKRHIF*LGQSILRTKLFFLP FIFLFMIYISIIL

Frame 1

CGILGSPVTAAAGSRRALPGHRPVVLGREKAGH FPT*LPALGLECVHGEVPGELYPGGDSGAVSTE SPGTCQQKHSPTDMCPERRRCHYWRRCTKSFF*H QCGK*GRQDSLARITTEPCC*SAQRFVAPTSR* GK*SISVKKKKNVPLEVIRT*GNQYAYYARPCS YY**PVSIYSSFRGRS*VLQGGTLWNEK*NTSSC A*KRRAPATAKISKTRGDTEGTNTSGCIRKHAQ FLDYNR*RFWGGRNLQKTIFP*QCRFWQSCICW *AARTGEAKTYL*GKV*N*GIPIEVFEERLS*IS ENL*RS*RATKA*RISSGC*YL*PCWWSLFEMC SA*SCSFPNPY*CSYADHRKTG*RKR*LDVCTS FRKEQLGRYAAKRSKCL*TGETSFANI*GSQF*K NQGFNPV*PVEEGAGEAGGAT*KRTCISAREKG H*ERHDEKGNNERKGVHGIKDVDLVSEYCPTGG PGGKGYKGKDFSY*STGGNSKPAGFSGNGCHKGV WRNALSAE*NQHGEG*GRKGAQRVQSKN*QGS* N*RSGNREIENRTG*KQTTLGTGAAEGSPGQRG VPETNRTAGRI*APTAPHQTGKR*HSAEL*QGSK GPSPSGPAKRAGADTEDTANGGPA*QN*K*TVF VADLPEYIFDKVKGRMLYISQETGTNLSKNQI* NSSTQSRKKVYI**IGKVTEKK*RIGGTVCPAWE ST*DDEAKAKAAG*AQPGHSPAAGAAPQQAEPA SPGEAEPVGRGGPAADPVTQHATI*LLTWMEQS EINDLQRDIYIHLV*T*YATTHHDLPRVTPPQPA VGLVLINAGAVPARSRAGAGV*L*LSRAPGVCF QKWLEVMCLNLLIRMLGYTYDFQ*MNFLKT*VV MFPFYLVVMNKTHQKGTYFN*AKVY*GPNFFSCH SFFFS*SIYQSSF

Frame 2

AGF*APL*QPRQEAGGRSPATGLLFSEGRKLDI SPRNSQLWA*SACMAKSPENSTLEEILGQYQRS LREHASRSIHQLTCALKEGDVTIGEDAPNLSFS TSVGNEDARTAWPELQQSHAVNQLKDLLRQQADK ESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVH TINDQSQYIHHLEAEVKFCKEELSGMKNKIQVV VLENEGLQQQLKSQRQEETLREQTLLDASGNMHN SWITTGEDSGVGETSKRPFSHDNADFGKAASAG EQLELEKLKLTYEEKCEIEESQLKFLRNDLAEY QRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCA QHEAVLSQTHTNVHMQTIERLVKERDDLMSALV SVRSSLADTQQREASAYEQVKQVLQISEEANFE KTKALIQCDQLRKELERQAERLEKELASQQEKRA IEKDMMKKEITKEREYMGSKMLILSQNIAQLEA QVEKVTKEKISAINQLEEIQSQLASREMDVTKV CGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLE IKDQEIEKLRIELDESKQHLEQEQQKAALAREE CLRLTELLGESEHQLHLTRQEKDSIQQSFSKEA KAQALQAQQREQELTQKIQQMEAQHDKTENEQYL LLTSQNTFLTKLKEECCTLAKKLEQISQKTRSE IAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHG RVHETMKQRLRQLDKHSQATAQQLVQLLSKQNQL LLERQSLSEEVDRLRTQLPSMPQSDC*PGWNRV K*MIYKEIFTFIWFRLNMPQRTTTFPG*HRLSL QWGWSSSTRALSPHAVGLELESDSS*AELLVYVF RNGLKLCV*ICSFVC*VIHMIFNK*TF*RLEL* CFLFIL***TKHTKKAHILTRPKYIKDQTFFPA IHFSFHDLYINHPS