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Sequences

Sequence BRCA1.iAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGGGGTGACCCAG TCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAAT CCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGG TGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTC CATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGA TACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCT GGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTA AAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCA ATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAG AATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATG TGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTT GAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAA CATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATC ACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAA AAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGG TTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCT GGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAA CCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGT CTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAG CAAACACTTCCA

BRCA1-F1 (forward primer)
BRCA1-10 (reverse primer)
Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKRGDP VY*RKKNAE*A*F*SQRRCGQWKKPPRSKASKRI PGQKDLQGARNLLLWALHQHAHRSTGMDGTAVW CFCGEGAFIIHPWHRCPPNCGCAARCLDRGQWL PCNWADV*GTCGDPRVGVGQCSTLPVPGAGHLPD TPDPPQPLLTAASHRYRATGPQE*AYKVAFPGP GSSSHSSVLLLSWLLNILCTSA*KGLLAMQGSL KDFLLEVSLGNLP*AQNYGNFSPEKILKPFKRHQ LSKMLIHYLSALFFLFRLLLA*GWKHRVAWPQE NSWFP*VYFSKTLCSQRQRVRPFNGRRVLGIDY VT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KGNL KPGHGGSRL*SQHFGRPRWADHWRSGVRNQPGQ HGETPSLLKIQKLAGHGGGHL*SQLLRWLRQEN HFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKKKK RKMKLEEISKSLRYIC*ISKECVLKQQKIFKNR FPKTVF*FLISNK*NVYCCSSGI*SIPLKI*DL WHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC*N HTAS*IILLAEGRKSVFHKPIIQDCL*LLEGLG LP*PPQCARAVKT*LYKIRFVNVVLLTLQINLV ANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKGVTQ SIKERKMLNEHDFEVRGDVVNGRNHQGPKRARE SQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCG ASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGF HAIGQMCEAPVVTREWVLDSVALYQCQELDTYL IPQIPHSHY*LQPATGTEPQDPKNELTKWPFQAL GAPLTLQSFYCPGY*IFYVHQPEKDFWLCKGPL KIFCLKSPLEICHEHKIMVIFHLRRF*NHLNAT N*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLKR IAGFPKFTSLKPCVHKGRESDPSMEGECLGSIM *LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKET *NLGMVAHACNPSTLGGQGGQITGGQEFETSLAN MVKPHLY*KYRN*PVMVVDTCNPSYSGG*GRRI TSAREVEVAVSQDHTTALQPG*Q*DCGSKKKKK KGK*N*KRFLKV*DIFARFLKNVF*NSRRFSRTG FQRQSSNSSLVISKMFIVVALVYNPFLLKYKTS GMNISYL*NDRSHQEGSCCFL*GDFFPLLPVAE TIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD*V FPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW* QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKG*PS LLKKEKC*MSMILKSEEMWSMEETTKVQSEQEN PRTERSSGG*KSVAMGPSPTCPQINWNGWYSCV VLLW*RSFHHSPLAQVSTQLWLCSQMPGQRTMAS MQLGRCVRHLW*PESGCWTV*HSTSARSWTPT* YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSRP WELLSLFSPSTVLATKYFMYISLKRTSGYARVP* RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*TPP IEQDADSLFISPILSIQAVVGLGLEAQSGLASR E*LVSLSLLL*NPVFTKAESQTLQWKESAWDRLC DLKSE*SLGSSQMLEWNIGEEILRQVLEMKRKL ETWAWWLTPVIPALWEAKVGRSLEVRSSKPAWP TW*NPISTKNTEISRSWWWTPVIPATQVAKAGES LQPGRWRLQ*AKIIPRHSSLGDSETVAQKKKKK KENETRRDF*KSEIYLLDF*RMCSKTAEDFQEP VSKDSLLIPH***VKCLLL*LWYIIHSS*NIRPL A*IFHIYKMTDPTRKEAVAFFEVIFFLCSLLLK PYSFINNFAC*RKKKCFS*THYPGLFIAVGRTR SSLAPPVCKGSEDLIVQNTFCKCCAVNTANKLGS KHF

Sequence BRCA1.hAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGATGCTGAGTTT GTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGC TGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAG GGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTT TCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTG AGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCA CTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAG TCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTC CCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCAT TATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTT ACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCC TTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCA CGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTAC TAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGG TTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAG ATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAAT TCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTAT AAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATT TTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTG CAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA

BRCA1-F1 (forward primer)
BRCA1-10 (reverse primer)
Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKRC*V CV*TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNA E*A*F*SQRRCGQWKKPPRSKASKRIPGQKDLQ GARNLLLWALHQHAHRSTGMDGTAVWCFCGEGA FIIHPWHRCPPNCGCAARCLDRGQWLPCNWADV* GTCGDPRVGVGQCSTLPVPGAGHLPDTPDPPQP LLTAASHRYRATGPQE*AYKVAFPGPGSSSHSS VLLLSWLLNILCTSA*KGLLAMQGSLKDFLLEVS LGNLP*AQNYGNFSPEKILKPFKRHQLSKMLIH YLSALFFLFRLLLA*GWKHRVAWPQENSWFP*V YFSKTLCSQRQRVRPFNGRRVLGIDYVT*SQNSP WAVLKCWSGTLGRKF*GRY*K*KGNLKPGHGGS RL*SQHFGRPRWADHWRSGVRNQPGQHGETPSL LKIQKLAGHGGGHL*SQLLRWLRQENHFSPGGGG CSEPRSYHGTPAWVTVRLWLKKKKKKRKMKLEE ISKSLRYIC*ISKECVLKQQKIFKNRFPKTVF* FLISNK*NVYCCSSGI*SIPLKI*DLWHEYFISI K*QIPPGRKLLLSLR*FFSFAPCC*NHTAS*II LLAEGRKSVFHKPIIQDCL*LLEGLGLP*PPQC ARAVKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKDAEF VCERTLKYFLGIAGGKWVVSYFWVTQSIKERKM LNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFR GLEICCYGPFTNMPTDQLEWMVQLCGASVVKEL SSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMC EAPVVTREWVLDSVALYQCQELDTYLIPQIPHSH Y*LQPATGTEPQDPKNELTKWPFQALGAPLTLQ SFYCPGY*IFYVHQPEKDFWLCKGPLKIFCLKS PLEICHEHKIMVIFHLRRF*NHLNATN*ARC*FI IYQPYSFYSGCCWLRAGSTEWLGLKRIAGFPKF TSLKPCVHKGRESDPSMEGECLGSIM*LKVRIV LGQFSNVGVEHWGGNSEAGIRNEKET*NLGMVAH ACNPSTLGGQGGQITGGQEFETSLANMVKPHLY *KYRN*PVMVVDTCNPSYSGG*GRRITSAREVE VAVSQDHTTALQPG*Q*DCGSKKKKKKGK*N*KR FLKV*DIFARFLKNVF*NSRRFSRTGFQRQSSN SSLVISKMFIVVALVYNPFLLKYKTSGMNISYL *NDRSHQEGSCCFL*GDFFPLLPVAETIQLHK*F CLLKEEKVFFINPLSRTVYSCWKD*VFPSPPSV QGQ*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKMLSL CVNGH*NIF*ELREENG*LAISG*PSLLKKEKC *MSMILKSEEMWSMEETTKVQSEQENPRTERSS GG*KSVAMGPSPTCPQINWNGWYSCVVLLW*RSF HHSPLAQVSTQLWLCSQMPGQRTMASMQLGRCV RHLW*PESGCWTV*HSTSARSWTPT*YPRSPTA TTDCSQPQVQSHRTPRMSLQSGLSRPWELLSLFS PSTVLATKYFMYISLKRTSGYARVP*RFSA*SL PWKSAMSTKLW*FFT*EDFKTI*TPPIEQDADS LFISPILSIQAVVGLGLEAQSGLASRE*LVSLSL LL*NPVFTKAESQTLQWKESAWDRLCDLKSE*S LGSSQMLEWNIGEEILRQVLEMKRKLETWAWWL TPVIPALWEAKVGRSLEVRSSKPAWPTW*NPIST KNTEISRSWWWTPVIPATQVAKAGESLQPGRWR LQ*AKIIPRHSSLGDSETVAQKKKKKKENETRR DF*KSEIYLLDF*RMCSKTAEDFQEPVSKDSLLI PH***VKCLLL*LWYIIHSS*NIRPLA*IFHIY KMTDPTRKEAVAFFEVIFFLCSLLLKPYSFINN FAC*RKKKCFS*THYPGLFIAVGRTRSSLAPPVC KGSEDLIVQNTFCKCCAVNTANKLGSKHF