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Sequences

Sequence BRCA1.iAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGGGGTGACCCAG TCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAAT CCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGG TGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTC CATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGA TACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCT GGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTA AAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCA ATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAG AATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATG TGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTT GAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAA CATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATC ACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAA AAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGG TTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCT GGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAA CCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGT CTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAG CAAACACTTCCA

BRCA1-F1 (forward primer)
BRCA1-10 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKRGDP VY*RKKNAE*A*F*SQRRCGQWKKPPRSKASKRI PGQKDLQGARNLLLWALHQHAHRSTGMDGTAVW CFCGEGAFIIHPWHRCPPNCGCAARCLDRGQWL PCNWADV*GTCGDPRVGVGQCSTLPVPGAGHLPD TPDPPQPLLTAASHRYRATGPQE*AYKVAFPGP GSSSHSSVLLLSWLLNILCTSA*KGLLAMQGSL KDFLLEVSLGNLP*AQNYGNFSPEKILKPFKRHQ LSKMLIHYLSALFFLFRLLLA*GWKHRVAWPQE NSWFP*VYFSKTLCSQRQRVRPFNGRRVLGIDY VT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KGNL KPGHGGSRL*SQHFGRPRWADHWRSGVRNQPGQ HGETPSLLKIQKLAGHGGGHL*SQLLRWLRQEN HFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKKKK RKMKLEEISKSLRYIC*ISKECVLKQQKIFKNR FPKTVF*FLISNK*NVYCCSSGI*SIPLKI*DL WHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC*N HTAS*IILLAEGRKSVFHKPIIQDCL*LLEGLG LP*PPQCARAVKT*LYKIRFVNVVLLTLQINLV ANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKGVTQ SIKERKMLNEHDFEVRGDVVNGRNHQGPKRARE SQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCG ASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGF HAIGQMCEAPVVTREWVLDSVALYQCQELDTYL IPQIPHSHY*LQPATGTEPQDPKNELTKWPFQAL GAPLTLQSFYCPGY*IFYVHQPEKDFWLCKGPL KIFCLKSPLEICHEHKIMVIFHLRRF*NHLNAT N*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLKR IAGFPKFTSLKPCVHKGRESDPSMEGECLGSIM *LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKET *NLGMVAHACNPSTLGGQGGQITGGQEFETSLAN MVKPHLY*KYRN*PVMVVDTCNPSYSGG*GRRI TSAREVEVAVSQDHTTALQPG*Q*DCGSKKKKK KGK*N*KRFLKV*DIFARFLKNVF*NSRRFSRTG FQRQSSNSSLVISKMFIVVALVYNPFLLKYKTS GMNISYL*NDRSHQEGSCCFL*GDFFPLLPVAE TIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD*V FPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW* QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKG*PS LLKKEKC*MSMILKSEEMWSMEETTKVQSEQEN PRTERSSGG*KSVAMGPSPTCPQINWNGWYSCV VLLW*RSFHHSPLAQVSTQLWLCSQMPGQRTMAS MQLGRCVRHLW*PESGCWTV*HSTSARSWTPT* YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSRP WELLSLFSPSTVLATKYFMYISLKRTSGYARVP* RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*TPP IEQDADSLFISPILSIQAVVGLGLEAQSGLASR E*LVSLSLLL*NPVFTKAESQTLQWKESAWDRLC DLKSE*SLGSSQMLEWNIGEEILRQVLEMKRKL ETWAWWLTPVIPALWEAKVGRSLEVRSSKPAWP TW*NPISTKNTEISRSWWWTPVIPATQVAKAGES LQPGRWRLQ*AKIIPRHSSLGDSETVAQKKKKK KENETRRDF*KSEIYLLDF*RMCSKTAEDFQEP VSKDSLLIPH***VKCLLL*LWYIIHSS*NIRPL A*IFHIYKMTDPTRKEAVAFFEVIFFLCSLLLK PYSFINNFAC*RKKKCFS*THYPGLFIAVGRTR SSLAPPVCKGSEDLIVQNTFCKCCAVNTANKLGS KHF

Sequence BRCA1.oAug05

BRCA1-F1 (forward primer)
BRCA1-10 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWFIGTERNGFICSSR*RSTKCH*CYAENLRVS HLSGVDQGTCLHKV*PHILQILHAETSQPEERA FTVSFM*E*YNQKEPTRKYEI*STC*RAIENHLC FSA*HRFGVCKQL*FCKKGK*LS*TSKR*SFYH PKYGLQKPCQKTSTE*TRKSFLAGNQSQCPTL* PWNCENSEDKAADTTSKDVCLH*IGI*FF*RYR* *GNLLQCGRSRIVTNHPSRNQG*NQFGFCKKGC L*IF*DGCNKY*TSSTQ***FEHH*EACS*EAS RKVSG*SSIWV*E*NKRL*RLLRAILSE*HFNHS AEGYHAT*PDKAPAGNG*TRSCVRTAWEPAF*Q LPFHHK*LFCP*GPAKSRTKHIRKSINFTEK** IPYKPESRRPFC*QV*GVCR*FYQ*K*RTRSGKV IPF*MPIIR**VVHAQLLWESSE*KLPISRGAH *GC*CGGATAGRVWATRFDGNILLAKARSRGNP LPGIWNQPLL**P*I*SF*RQSPRVSSCWQHTIF NLCIESSPIESCRICPESSCCSYY*YCWV*CNG RKCEQGEARIDSFNRKGQQKNVHGGVWPDPRRI YARVQVCQKTPHHFN*SNY*RDYSCCYENRC*VC V*TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNA E*A*F*SQRRCGQWKKPPRSKASKRIPGQKDLQ GARNLLLWALHQHAHRVSTQLWLCSQMPGQRTMA SMQLGRCVRHLW*PESGCWTV*HSTSARSWTPT *YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSR PWELLSLFSPSTVLATKYFMYISLKRTSGYARVP *RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*TP PIEQDADSLFISPILSIQAVVGLGLEAQSGLAS RE*LVSLSLLL*NPVFTKAESQTLQWKESAWDRL CDLKSE*SLGSSQMLEWNIGEEILRQVLEMKRK LETWAWWLTPVIPALWEAKVGRSLEVRSSKPAW PTW*NPISTKNTEISRSWWWTPVIPATQVAKAGE SLQPGRWRLQ*AKIIPRHSSLGDSETVAQKKKK KKENETRRDF*KSEIYLLDF*RMCSKTAEDFQE PVSKDSLLIPH***VKCLLL*LWYIIHSS*NIRP LA*IFHIYKMTDPTRKEAVAFFEVIFFLCSLLL KPYSFINNFAC*RKKKCFS*THYPGLFIAVGRT RSSLAPPVCKGSEDLIVQNTFCKCCAVNTANKLG SKHF

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSGSLEQKEMDLSALRVEEVQNVINAMQKILECP ICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGP SQCPLCKNDITKRSLQESTRFSQLVEELLKIIC AFQLDTGLEYANSYNFAKKENNSPEHLKDEVSII QSMGYRNRAKRLLQSEPENPSLQETSLSVQLSN LGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTV NKATYCSVGDQELLQITPQGTRDEISLDSAKKAA CEFSETDVTNTEHHQPSNNDLNTTEKRAAERHP EKYQGEAASGCESETSVSEDCSGLSSQSDILTT QQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNS YPSIISDSSALEDLRNPEQSTSEKVLTSQKSSE YPISQNPEGLSADKFEVSADSSTSKNKEPGVER SSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELI KVVDVEEQQLEESGPHDLTETSYLPRQDLEGTP YLESGISLFSDDPESDPSEDRAPESARVGNIPS STSALKVPQLKVAESAQSPAAAHTTDTAGYNAME ESVSREKPELTASTERVNKRMSMVVSGLTPEEF MLVYKFARKHHITLTNLITEETTHVVMKTDAEF VCERTLKYFLGIAGGKWVVSYFWVTQSIKERKML NEHDFEVRGDVVNGRNHQGPKRARESQDRKIFR GLEICCYGPFTNMPTGCPPNCGCAARCLDRGQW LPCNWADV*GTCGDPRVGVGQCSTLPVPGAGHLP DTPDPPQPLLTAASHRYRATGPQE*AYKVAFPG PGSSSHSSVLLLSWLLNILCTSA*KGLLAMQGS LKDFLLEVSLGNLP*AQNYGNFSPEKILKPFKRH QLSKMLIHYLSALFFLFRLLLA*GWKHRVAWPQ ENSWFP*VYFSKTLCSQRQRVRPFNGRRVLGID YVT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KGN LKPGHGGSRL*SQHFGRPRWADHWRSGVRNQPG QHGETPSLLKIQKLAGHGGGHL*SQLLRWLRQE NHFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKKK KRKMKLEEISKSLRYIC*ISKECVLKQQKIFKN RFPKTVF*FLISNK*NVYCCSSGI*SIPLKI*D LWHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC* NHTAS*IILLAEGRKSVFHKPIIQDCL*LLEGL GLP*PPQCARAVKT*LYKIRFVNVVLLTLQINL VANTS

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALVHWNRKKWIYLLFALKKYKMSLMLCRKS*SV PSVWS*SRNLSPQSVTTYFANFAC*NFSTRRKGL HSVLYVRMI*PKGAYKKVRDLVNLLKSY*KSFV LFSLTQVWSMQTAIILQKRKITLLNI*KMKFLS SKVWATETVPKDFYRVNPKILPCRKPVSVSNSLT LEL*EL*GQSSGYNLKRRLSTLNWDLILLKIPL IRQLIAVWEIKNCYKSPLKEPGMKSVWILQKRL LVNFLRRM*QILNIINPVIMI*TPLRSVQLRGIQ KSIRVKQHLGVRVKQASLKTAQGYPLRVTF*PL SRGIPCNIT**SSSRKWLN*KLC*NSMGASLLT ATLPS*VTLLPLRTCEIQNKAHQKKY*LHRKVVN TL*ARIQKAFLLTSLRCLQIVLPVKIKNQEWKG HPLLNAHH*MIGGTCTVALGVFRIETTHLKRSS LRLLMWRSNSWKSLGHTI*RKHLTCQGKI*REPL TWNLESASSLMTLNLILLKTEPQSQLVLATYHL QPLH*KFPN*KLQNLPRVQLLLILLILLGIMQW KKV*AGRSQN*QLQQKGSTKECPWWCLA*PQKNL CSCTSLPENTTSL*LI*LLKRLLMLL*KQMLSL CVNGH*NIF*ELREENG*LAISG*PSLLKKEKC *MSMILKSEEMWSMEETTKVQSEQENPRTERSSG G*KSVAMGPSPTCPQGVHPIVVVQPDAWTEDNG FHAIGQMCEAPVVTREWVLDSVALYQCQELDTY LIPQIPHSHY*LQPATGTEPQDPKNELTKWPFQA LGAPLTLQSFYCPGY*IFYVHQPEKDFWLCKGP LKIFCLKSPLEICHEHKIMVIFHLRRF*NHLNA TN*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLK RIAGFPKFTSLKPCVHKGRESDPSMEGECLGSI M*LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKE T*NLGMVAHACNPSTLGGQGGQITGGQEFETSLA NMVKPHLY*KYRN*PVMVVDTCNPSYSGG*GRR ITSAREVEVAVSQDHTTALQPG*Q*DCGSKKKK KKGK*N*KRFLKV*DIFARFLKNVF*NSRRFSRT GFQRQSSNSSLVISKMFIVVALVYNPFLLKYKT SGMNISYL*NDRSHQEGSCCFL*GDFFPLLPVA ETIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD* VFPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW *QTLP

Sequence BRCA1.iAug05

Frame 0

Frame 1

Frame 2

Sequence BRCA1.oAug05

Frame 0

Frame 1

Frame 2

Sequence BRCA1.lAug05

Frame 0

Frame 1

Frame 2