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Sequences

Sequence BRCA1.pAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA
GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA
GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA
TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC
TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC
TCT
AACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGTGAAGCAGCATCTGGGTGTG
AGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAA
GCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTT
GAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTT
CTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGA
TAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTG
GAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTG
ATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAA
AGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTG
ACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACC
ACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGG
AATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGAT
GTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCA
CCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCC
AATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTG
GACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGC
CACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTA
TGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATG
GTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCT
GTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAG
AGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACAT
TGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAG
GTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGT
GGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTC
CAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGAT
TTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGT
TGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGT
TGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCA
TAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATA
CGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR
VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE
RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH
LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF
YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT
L
*PWNCENSEDKAADTTSKDVCLH*IG*SSIWV*
E*NKRL*RLLRAILSE*HFNHSAEGYHAT*PDK
APAGNG*TRSCVRTAWEPAF*QLPFHHK*LFCP
*GPAKSRTKHIRKSSINFTEK**IPYKPESRRPF
C*QV*GVCR*FYQ*K*RTRSGKVIPF*MPIIR*
*VVHAQLLWESSE*KLPISRGAH*GC*CGGATA
GRVWATRFDGNILLAKARSRGNPLPGIWNQPLL*
*P*I*SF*RQSPRVSSCWQHTIFNLCIESSPIE
SCRICPESSCCSYY*YCWV*CNGRKCEQGEARI
DSFNRKGQQKNVHGGVWPDPRRIYARVQVCQKTP
HHFN*SNY*RDYSCCYENRC*VCV*TDTEIFSR
NCGRKMGS*LFLGDPVY*RKKNAE*A*F*SQRR
CGQWKKPPRSKASKRIPGQKDLQGARNLLLWALH
QHAHRSTGMDGTAVWCFCGEGAFIIHPWHRCPP
NCGCAARCLDRGQWLPCNWADV*GTCGDPRVGV
GQCSTLPVPGAGHLPDTPDPPQPLLTAASHRYRA
TGPQE*AYKVAFPGPGSSSHSSVLLLSWLLNIL
CTSA*KGLLAMQGSLKDFLLEVSLGNLP*AQNY
GNFSPEKILKPFKRHQLSKMLIHYLSALFFLFRL
LLA*GWKHRVAWPQENSWFP*VYFSKTLCSQRQ
RVRPFNGRRVLGIDYVT*SQNSPWAVLKCWSGT
LGRKF*GRY*K*KGNLKPGHGGSRL*SQHFGRPR
WADHWRSGVRNQPGQHGETPSLLKIQKLAGHGG
GHL*SQLLRWLRQENHFSPGGGGCSEPRSYHGT
PAWVTVRLWLKKKKKKRKMKLEEISKSLRYIC*I
SKECVLKQQKIFKNRFPKTVF*FLISNK*NVYC
CSSGI*SIPLKI*DLWHEYFISIK*QIPPGRKL
LLSLR*FFSFAPCC*NHTAS*IILLAEGRKSVFH
KPIIQDCL*LLEGLGLP*PPQCARAVKT*LYKI
RFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE
CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK
GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI
ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS
IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL
S
NLGTVRTLRTKQRIQPQKTSVYIELGEAASGC
ESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIK
LQQEMAELEAVLEQHGSQPSNSYPSIISDSSAL
EDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGL
SADKFEVSADSSTSKNKEPGVERSSPSKCPSLDD
RWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQL
EESGPHDLTETSYLPRQDLEGTPYLESGISLFS
DDPESDPSEDRAPESARVGNIPSSTSALKVPQLK
VAESAQSPAAAHTTDTAGYNAMEESVSREKPEL
TASTERVNKRMSMVVSGLTPEEFMLVYKFARKH
HITLTNLITEETTHVVMKTDAEFVCERTLKYFLG
IAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGD
VVNGRNHQGPKRARESQDRKIFRGLEICCYGPF
TNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHP
IVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVL
DSVALYQCQELDTYLIPQIPHSHY*LQPATGTE
PQDPKNELTKWPFQALGAPLTLQSFYCPGY*IFY
VHQPEKDFWLCKGPLKIFCLKSPLEICHEHKIM
VIFHLRRF*NHLNATN*ARC*FIIYQPYSFYSG
CCWLRAGSTEWLGLKRIAGFPKFTSLKPCVHKGR
ESDPSMEGECLGSIM*LKVRIVLGQFSNVGVEH
WGGNSEAGIRNEKET*NLGMVAHACNPSTLGGQ
GGQITGGQEFETSLANMVKPHLY*KYRN*PVMVV
DTCNPSYSGG*GRRITSAREVEVAVSQDHTTAL
QPG*Q*DCGSKKKKKKGK*N*KRFLKV*DIFAR
FLKNVF*NSRRFSRTGFQRQSSNSSLVISKMFIV
VALVYNPFLLKYKTSGMNISYL*NDRSHQEGSC
CFL*GDFFPLLPVAETIQLHK*FCLLKEEKVFF
INPLSRTVYSCWKD*VFPSPPSVQGQ*RLDCTKY
VL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS*
SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK
GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS
FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF
LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS
L
TLEL*EL*GQSSGYNLKRRLSTLNWVKQHLGV
RVKQASLKTAQGYPLRVTF*PLSRGIPCNIT**
SSSRKWLN*KLC*NSMGASLLTATLPS*VTLLPL
RTCEIQNKAHQKKQY*LHRKVVNTL*ARIQKAF
LLTSLRCLQIVLPVKIKNQEWKGHPLLNAHH*M
IGGTCTVALGVFRIETTHLKRSSLRLLMWRSNSW
KSLGHTI*RKHLTCQGKI*REPLTWNLESASSL
MTLNLILLKTEPQSQLVLATYHLQPLH*KFPN*
KLQNLPRVQLLLILLILLGIMQWKKV*AGRSQN*
QLQQKGSTKECPWWCLA*PQKNLCSCTSLPENT
TSL*LI*LLKRLLMLL*KQMLSLCVNGH*NIF*
ELREENG*LAISG*PSLLKKEKC*MSMILKSEEM
WSMEETTKVQSEQENPRTERSSGG*KSVAMGPS
PTCPQINWNGWYSCVVLLW*RSFHHSPLAQVST
QLWLCSQMPGQRTMASMQLGRCVRHLW*PESGCW
TV*HSTSARSWTPT*YPRSPTATTDCSQPQVQS
HRTPRMSLQSGLSRPWELLSLFSPSTVLATKYF
MYISLKRTSGYARVP*RFSA*SLPWKSAMSTKLW
*FFT*EDFKTI*TPPIEQDADSLFISPILSIQA
VVGLGLEAQSGLASRE*LVSLSLLL*NPVFTKA
ESQTLQWKESAWDRLCDLKSE*SLGSSQMLEWNI
GEEILRQVLEMKRKLETWAWWLTPVIPALWEAK
VGRSLEVRSSKPAWPTW*NPISTKNTEISRSWW
WTPVIPATQVAKAGESLQPGRWRLQ*AKIIPRHS
SLGDSETVAQKKKKKKENETRRDF*KSEIYLLD
F*RMCSKTAEDFQEPVSKDSLLIPH***VKCLL
L*LWYIIHSS*NIRPLA*IFHIYKMTDPTRKEAV
AFFEVIFFLCSLLLKPYSFINNFAC*RKKKCFS
*THYPGLFIAVGRTRSSLAPPVCKGSEDLIVQN
TFCKCCAVNTANKLGSKHF

Sequence BRCA1.nAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA
GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA
GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA
TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC
TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC
TCT
AACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGCTGCTTGTGAATTTTCTGAGA
CGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGG
GTGA
AGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATG
CAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAA
GTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCA
GAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAA
TGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATG
TGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGG
AATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTG
AAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCA
GGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAA
GTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACA
CTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATT
TTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTG
TTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTT
GGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGA
CCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCC
AGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCT
GGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCC
ATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTA
TTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCC
TGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAA
ATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAG
CACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAAT
TAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAG
ATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTG
AGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATA
AGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCC
ACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGA
AGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGA
CTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR
VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE
RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH
LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF
YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT
L
*PWNCENSEDKAADTTSKDVCLH*IGCL*IF*D
GCNKY*TSSTQ***FEHH*EACS*EASRKVSG
*
SSIWV*E*NKRL*RLLRAILSE*HFNHSAEGYH
AT*PDKAPAGNG*TRSCVRTAWEPAF*QLPFHHK
*LFCP*GPAKSRTKHIRKSSINFTEK**IPYKP
ESRRPFC*QV*GVCR*FYQ*K*RTRSGKVIPF*
MPIIR**VVHAQLLWESSE*KLPISRGAH*GC*C
GGATAGRVWATRFDGNILLAKARSRGNPLPGIW
NQPLL**P*I*SF*RQSPRVSSCWQHTIFNLCI
ESSPIESCRICPESSCCSYY*YCWV*CNGRKCEQ
GEARIDSFNRKGQQKNVHGGVWPDPRRIYARVQ
VCQKTPHHFN*SNY*RDYSCCYENRC*VCV*TD
TEIFSRNCGRKMGS*LFLGDPVY*RKKNAE*A*F
*SQRRCGQWKKPPRSKASKRIPGQKDLQGARNL
LLWALHQHAHRSTGMDGTAVWCFCGEGAFIIHP
WHRCPPNCGCAARCLDRGQWLPCNWADV*GTCGD
PRVGVGQCSTLPVPGAGHLPDTPDPPQPLLTAA
SHRYRATGPQE*AYKVAFPGPGSSSHSSVLLLS
WLLNILCTSA*KGLLAMQGSLKDFLLEVSLGNLP
*AQNYGNFSPEKILKPFKRHQLSKMLIHYLSAL
FFLFRLLLA*GWKHRVAWPQENSWFP*VYFSKT
LCSQRQRVRPFNGRRVLGIDYVT*SQNSPWAVLK
CWSGTLGRKF*GRY*K*KGNLKPGHGGSRL*SQ
HFGRPRWADHWRSGVRNQPGQHGETPSLLKIQK
LAGHGGGHL*SQLLRWLRQENHFSPGGGGCSEPR
SYHGTPAWVTVRLWLKKKKKKRKMKLEEISKSL
RYIC*ISKECVLKQQKIFKNRFPKTVF*FLISN
K*NVYCCSSGI*SIPLKI*DLWHEYFISIK*QIP
PGRKLLLSLR*FFSFAPCC*NHTAS*IILLAEG
RKSVFHKPIIQDCL*LLEGLGLP*PPQCARAVK
T*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE
CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK
GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI
ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS
IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL
S
NLGTVRTLRTKQRIQPQKTSVYIELAACEFSE
TDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQ
GE
AASGCESETSVSEDCSGLSSQSDILTTQQRDTM
QHNLIKLQQEMAELEAVLEQHGSQPSNSYPSII
SDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQ
NPEGLSADKFEVSADSSTSKNKEPGVERSSPSK
CPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVD
VEEQQLEESGPHDLTETSYLPRQDLEGTPYLESG
ISLFSDDPESDPSEDRAPESARVGNIPSSTSAL
KVPQLKVAESAQSPAAAHTTDTAGYNAMEESVS
REKPELTASTERVNKRMSMVVSGLTPEEFMLVYK
FARKHHITLTNLITEETTHVVMKTDAEFVCERT
LKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHD
FEVRGDVVNGRNHQGPKRARESQDRKIFRGLEIC
CYGPFTNMPTDQLEWMVQLCGASVVKELSSFTL
GTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVV
TREWVLDSVALYQCQELDTYLIPQIPHSHY*LQP
ATGTEPQDPKNELTKWPFQALGAPLTLQSFYCP
GY*IFYVHQPEKDFWLCKGPLKIFCLKSPLEIC
HEHKIMVIFHLRRF*NHLNATN*ARC*FIIYQPY
SFYSGCCWLRAGSTEWLGLKRIAGFPKFTSLKP
CVHKGRESDPSMEGECLGSIM*LKVRIVLGQFS
NVGVEHWGGNSEAGIRNEKET*NLGMVAHACNPS
TLGGQGGQITGGQEFETSLANMVKPHLY*KYRN
*PVMVVDTCNPSYSGG*GRRITSAREVEVAVSQ
DHTTALQPG*Q*DCGSKKKKKKGK*N*KRFLKV*
DIFARFLKNVF*NSRRFSRTGFQRQSSNSSLVI
SKMFIVVALVYNPFLLKYKTSGMNISYL*NDRS
HQEGSCCFL*GDFFPLLPVAETIQLHK*FCLLKE
EKVFFINPLSRTVYSCWKD*VFPSPPSVQGQ*R
LDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS*
SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK
GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS
FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF
LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS
L
TLEL*EL*GQSSGYNLKRRLSTLNWLLVNFLR
RM*QILNIINPVIMI*TPLRSVQLRGIQKSIR
V
KQHLGVRVKQASLKTAQGYPLRVTF*PLSRGIPC
NIT**SSSRKWLN*KLC*NSMGASLLTATLPS*
VTLLPLRTCEIQNKAHQKKQY*LHRKVVNTL*A
RIQKAFLLTSLRCLQIVLPVKIKNQEWKGHPLLN
AHH*MIGGTCTVALGVFRIETTHLKRSSLRLLM
WRSNSWKSLGHTI*RKHLTCQGKI*REPLTWNL
ESASSLMTLNLILLKTEPQSQLVLATYHLQPLH*
KFPN*KLQNLPRVQLLLILLILLGIMQWKKV*A
GRSQN*QLQQKGSTKECPWWCLA*PQKNLCSCT
SLPENTTSL*LI*LLKRLLMLL*KQMLSLCVNGH
*NIF*ELREENG*LAISG*PSLLKKEKC*MSMI
LKSEEMWSMEETTKVQSEQENPRTERSSGG*KS
VAMGPSPTCPQINWNGWYSCVVLLW*RSFHHSPL
AQVSTQLWLCSQMPGQRTMASMQLGRCVRHLW*
PESGCWTV*HSTSARSWTPT*YPRSPTATTDCS
QPQVQSHRTPRMSLQSGLSRPWELLSLFSPSTVL
ATKYFMYISLKRTSGYARVP*RFSA*SLPWKSA
MSTKLW*FFT*EDFKTI*TPPIEQDADSLFISP
ILSIQAVVGLGLEAQSGLASRE*LVSLSLLL*NP
VFTKAESQTLQWKESAWDRLCDLKSE*SLGSSQ
MLEWNIGEEILRQVLEMKRKLETWAWWLTPVIP
ALWEAKVGRSLEVRSSKPAWPTW*NPISTKNTEI
SRSWWWTPVIPATQVAKAGESLQPGRWRLQ*AK
IIPRHSSLGDSETVAQKKKKKKENETRRDF*KS
EIYLLDF*RMCSKTAEDFQEPVSKDSLLIPH***
VKCLLL*LWYIIHSS*NIRPLA*IFHIYKMTDP
TRKEAVAFFEVIFFLCSLLLKPYSFINNFAC*R
KKKCFS*THYPGLFIAVGRTRSSLAPPVCKGSED
LIVQNTFCKCCAVNTANKLGSKHF

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