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Sequences

Sequence BRCA1.nAug05

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

Frame 1

Frame 2

Sequence BRCA1.dAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGCTGCTTGTGAATTTTCTGAGA CGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAG TTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATG AATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATA GGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCC TAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGAC TCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACT TACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAAC CTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCC CTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAA TGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAA TGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAA TTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATC TAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAG AAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGCGATACTTTC CCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAA AAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGT AGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAA CCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGT ATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTC AAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCA AAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTG TGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGG ACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAAT CTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACA TTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAG TGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGT CTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAG ATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAG TTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACAT TTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTAT TTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAA GAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCT TCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCC AGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAA CTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGG GATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTT CCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCC TATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCC CCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGG TTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCT GGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACC TCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAA GTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCT CGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGT GAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATG AGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCT AGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCA TTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCAC CTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTG ACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTC TACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGG AAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTA TCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCT CTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGC AGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTG TAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAA TACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAG TGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCT AAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCA TTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATG ACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTT GCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGG CAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGCL*IF*D GCNKY*TSSTQ***FEHH*EACS*EASRKVSG* FCFKLACGAMWHKYSCQLITA*EQQFITH*RQN ECRKG*IL**KQTAWLSKEPT*QMGWK*GNM*** ADSQHRKKGRSEC*SPV*EKRME*AETAMLRES *RY*RCSLDNTK*QHSES**VVFQK**TVRF** LT*WGV*IKCQSS*CIGRSK*GR*IFWFFRENRL TGQ*SS*GFNM*K*KSSLQISRE*Y*RQNIWEN LSEEGKPPQLKPCN*KSNYRSICY*ATDNTRAS PHK*IKA*KETYIRPSS*GFYQESRFGSSKDS*N DKSGN*PNGAEWSSDEYY**WS*E*NKR*FYSE *EKS*PNRITRKRICFQNES*TYKQQYKQYGTR IKYPQFKST*KE*AEEEVFYQAYSCA*TSSQ*KS KPT*LY*IAN**LF*Q*RDKEKKVQPNASQAQQ KPTTHGR*RTCNWSQEE*QAK*TDK*KT*QRYF PRAEVNKCTWFFY*VFKYQ*T*RICQS*PSKRRK RRETRNS*SV**C*RPQRSHVKWRKGFAN*KIC RE*QYFIGTWY*LWHSGKYLVTGS*HSREGKNR TK*MCESVCSI*KPQGTNSWLFQR**K*HRRL*V SIGT*S*PQSGNKHRNGRK*T*CSVFAEYIQGF KAPVICSVFKSRKCRRGMCNILCPLWVLKETKS KSHF*M*TKGRKSRKE*V*YQACTDS*YHCRLSC GWSER*AS**CQM*YQRRL*VLSIISVQRQRNW THYSK*TWTFTKPISYTTTFSHQVIC*N*M*EK SARGKL*GTFNVT*KRNGK*EHSKYSEHN*P**H *RKCF*RSQLKQY**SRFQY**SGLQY**NRFQ **KHSSRTR*KQRAKIECYA*IRGFAT*GL*TK SSWK*L*AS*NKKARI*RSSSDC*YRFLSISDFR *LRTAYGK*SCISGLF*DT**PVR*W*NKGRY* FC*K*H*GKFCCF*QKRPERRA*QES*PFHPYT FGSGLPKRGQEIRVLRRELI**G*RASLLPTLVI W*SKQYTFSVY*A*HRCYRVSV*EHRGEFIIIE E*LK*LQ*PGNIGKGISGTSP**GNKMFC*LVF FTVQ*IGRLDCKYKHPGSFLDWFFQTNEASV*KP GSWSE*QGIGFR**RKRNGLGRK*SRRAKHGFK LR*SSIWV*E*NKRL*RLLRAILSE*HFNHSAE GYHAT*PDKAPAGNG*TRSCVRTAWEPAF*QLPF HHK*LFCP*GPAKSRTKHIRKSSINFTEK**IP YKPESRRPFC*QV*GVCR*FYQ*K*RTRSGKVI PF*MPIIR**VVHAQLLWESSE*KLPISRGAH*G C*CGGATAGRVWATRFDGNILLAKARSRGNPLP GIWNQPLL**P*I*SF*RQSPRVSSCWQHTIFN LCIESSPIESCRICPESSCCSYY*YCWV*CNGRK CEQGEARIDSFNRKGQQKNVHGGVWPDPRRIYA RVQVCQKTPHHFN*SNY*RDYSCCYENRC*VCV *TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNAE* A*F*SQRRCGQWKKPPRSKASKRIPGQKDLQGA RNLLLWALHQHAHRSTGMDGTAVWCFCGEGAFI IHPWHRCPPNCGCAARCLDRGQWLPCNWADV*GT CGDPRVGVGQCSTLPVPGAGHLPDTPDPPQPLL TAASHRYRATGPQE*AYKVAFPGPGSSSHSSVL LLSWLLNILCTSA*KGLLAMQGSLKDFLLEVSLG NLP*AQNYGNFSPEKILKPFKRHQLSKMLIHYL SALFFLFRLLLA*GWKHRVAWPQENSWFP*VYF SKTLCSQRQRVRPFNGRRVLGIDYVT*SQNSPWA VLKCWSGTLGRKF*GRY*K*KGNLKPGHGGSRL *SQHFGRPRWADHWRSGVRNQPGQHGETPSLLK IQKLAGHGGGHL*SQLLRWLRQENHFSPGGGGCS EPRSYHGTPAWVTVRLWLKKKKKKRKMKLEEIS KSLRYIC*ISKECVLKQQKIFKNRFPKTVF*FL ISNK*NVYCCSSGI*SIPLKI*DLWHEYFISIK* QIPPGRKLLLSLR*FFSFAPCC*NHTAS*IILL AEGRKSVFHKPIIQDCL*LLEGLGLP*PPQCAR AVKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELAACEFSE TDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGS SVSNLHVEPCGTNTHASSLQHENSSLLLTKDRM NVEKAEFCNKSKQPGLARSQHNRWAGSKETCND RRTPSTEKKVDLNADPLCERKEWNKQKLPCSENP RDTEDVPWITLNSSIQKVNEWFSRSDELLGSDD SHDGESESNAKVADVLDVLNEVDEYSGSSEKID LLASDPHEALICKSERVHSKSVESNIEDKIFGKT YRKKASLPNLSHVTENLIIGAFVTEPQIIQERP LTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPE MINQGTNQTEQNGQVMNITNSGHENKTKGDSIQN EKNPNPIESLEKESAFKTKAEPISSSISNMELE LNIHNSKAPKKNRLRRKSSTRHIHALELVVSRN LSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSR NLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTF PELKLTNAPGSFTKCSNTSELKEFVNPSLPREE KEEKLETVKVSNNAEDPKDLMLSGERVLQTERSV ESSSISLVPGTDYGTQESISLLEVSTLGKAKTE PNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFK YPLGHEVNHSRETSIEMEESELDAQYLQNTFKVS KRQSFAPFSNPGNAEEECATFSAHSGSLKKQSP KVTFECEQKEENQGKNESNIKPVQTVNITAGFP VVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETG LITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKN LLEENFEEHSMSPEREMGNENIPSTVSTISRNN IRENVFKEASSSNINEVGSSTNEVGSSINEIGSS DENIQAELGRNRGPKLNAMLRLGVLQPEVYKQS LPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLIS DNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTS FAENDIKESSAVFSKSVQKGELSRSPSPFTHTH LAQGYRRGAKKLESSEENLSSEDEELPCFQHLL FGKVNNIPSQSTRHSTVATECLSKNTEENLLSLK NSLNDCSNQVILAKASQEHHLSEETKCSASLFS SQCSELEDLTANTNTQDPFLIGSSKQMRHQSES QGVGLSDKELVSDDEERGTGLEENNQEEQSMDSN LGEAASGCESETSVSEDCSGLSSQSDILTTQQR DTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYP SIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYP ISQNPEGLSADKFEVSADSSTSKNKEPGVERSS PSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIK VVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYL ESGISLFSDDPESDPSEDRAPESARVGNIPSST SALKVPQLKVAESAQSPAAAHTTDTAGYNAMEE SVSREKPELTASTERVNKRMSMVVSGLTPEEFML VYKFARKHHITLTNLITEETTHVVMKTDAEFVC ERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLN EHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGL EICCYGPFTNMPTDQLEWMVQLCGASVVKELSS FTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEA PVVTREWVLDSVALYQCQELDTYLIPQIPHSHY* LQPATGTEPQDPKNELTKWPFQALGAPLTLQSF YCPGY*IFYVHQPEKDFWLCKGPLKIFCLKSPL EICHEHKIMVIFHLRRF*NHLNATN*ARC*FIIY QPYSFYSGCCWLRAGSTEWLGLKRIAGFPKFTS LKPCVHKGRESDPSMEGECLGSIM*LKVRIVLG QFSNVGVEHWGGNSEAGIRNEKET*NLGMVAHAC NPSTLGGQGGQITGGQEFETSLANMVKPHLY*K YRN*PVMVVDTCNPSYSGG*GRRITSAREVEVA VSQDHTTALQPG*Q*DCGSKKKKKKGK*N*KRFL KV*DIFARFLKNVF*NSRRFSRTGFQRQSSNSS LVISKMFIVVALVYNPFLLKYKTSGMNISYL*N DRSHQEGSCCFL*GDFFPLLPVAETIQLHK*FCL LKEEKVFFINPLSRTVYSCWKD*VFPSPPSVQG Q*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWLLVNFLR RM*QILNIINPVIMI*TPLRSVQLRGIQKSIRV VLFQTCMWSHVAQILMPAHYSMRTAVYYSLKTE* M*KRLNSVIKANSLA*QGANITDGLEVRKHVMI GGLPAQKKR*I*MLIPCVREKNGISRNCHAQRI LEILKMFLG*H*IAAFRKLMSGFPEVMNC*VLMT HMMGSLNQMPK*LMYWTF*MR*MNILVLQRK*T YWPVILMRL*YVKVKEFTPNQ*RVILKTKYLGK PIGRRQASPT*AM*LKI*L*EHLLLSHR*YKSVP SQIN*SVKGDLHQAFILRILSRKQIWQFKRLLK **IRELTKRSRMVK**ILLIVVMRIKQKVILFR MRKILTQ*NHSKKNLLSKRKLNL*AAV*AIWNSN *ISTIQKHLKRIG*GGSLLPGIFMRLN**SVEI *AHLIVLNCKLIVVLAVKR*RKKSTTKCQSGTA ETYNSWKVKNLQLEPRRVTSQMNRQVKDMTAILS QS*S*QMHLVLLLSVQIPVNLKNLSILAFQEKK KKRN*KQLKCLIMLKTPKISC*VEKGFCKLKDL *RVAVFHWYLVLIMALRKVSRYWKLAL*GRQKQN QINV*VSVQHLKTPRD*FMVVPKIIEMTQKALS IHWDMKLTTVGKQA*KWKKVNLMLSICRIHSRF QSASHLLRFQIQEMQKRNVQHSLPTLGP*RNKVQ KSLLNVNKRKKIKERMSLISSLYRQLISLQAFL WLVRKISQLIMPNVVSKEALGFVYHLSSEATKL DSLLQINMDFYKTHIVYHHFFPSSHLLKLNVRKI C*RKTLRNIQCHLKEKWEMRTFQVQ*AQLAVIT LEKMFLKKPAQAILMK*VPVLMKWAPVLMK*VP VMKTFKQN*VETEGQN*MLCLD*GFCNLRSINKV FLEVIVSILK*KSKNMKK*FRLLIQISLHI*FQ IT*NSLWEVVMHLRFVLRHLMTC*MMVK*RKIL VLLKMTLRKVLLFLAKASRKESLAGVLALSPIHI WLRVTEEGPRN*SPQKRTYLVRMKSFPASNTCY LVK*TIYLLSLLGIAPLLPSVCLRTQRRIYYH* RIA*MTAVTR*YWQRHLRNITLVRKQNVLLACFL HSAVNWKT*LQIQTPRILS*LVLPNK*GISLKA RELV*VTRNWFQMMKKEERAWKKIIKKSKAWIQ T*VKQHLGVRVKQASLKTAQGYPLRVTF*PLSRG IPCNIT**SSSRKWLN*KLC*NSMGASLLTATL PS*VTLLPLRTCEIQNKAHQKKQY*LHRKVVNT L*ARIQKAFLLTSLRCLQIVLPVKIKNQEWKGHP LLNAHH*MIGGTCTVALGVFRIETTHLKRSSLR LLMWRSNSWKSLGHTI*RKHLTCQGKI*REPLT WNLESASSLMTLNLILLKTEPQSQLVLATYHLQP LH*KFPN*KLQNLPRVQLLLILLILLGIMQWKK V*AGRSQN*QLQQKGSTKECPWWCLA*PQKNLC SCTSLPENTTSL*LI*LLKRLLMLL*KQMLSLCV NGH*NIF*ELREENG*LAISG*PSLLKKEKC*M SMILKSEEMWSMEETTKVQSEQENPRTERSSGG *KSVAMGPSPTCPQINWNGWYSCVVLLW*RSFHH SPLAQVSTQLWLCSQMPGQRTMASMQLGRCVRH LW*PESGCWTV*HSTSARSWTPT*YPRSPTATT DCSQPQVQSHRTPRMSLQSGLSRPWELLSLFSPS TVLATKYFMYISLKRTSGYARVP*RFSA*SLPW KSAMSTKLW*FFT*EDFKTI*TPPIEQDADSLF ISPILSIQAVVGLGLEAQSGLASRE*LVSLSLLL *NPVFTKAESQTLQWKESAWDRLCDLKSE*SLG SSQMLEWNIGEEILRQVLEMKRKLETWAWWLTP VIPALWEAKVGRSLEVRSSKPAWPTW*NPISTKN TEISRSWWWTPVIPATQVAKAGESLQPGRWRLQ *AKIIPRHSSLGDSETVAQKKKKKKENETRRDF *KSEIYLLDF*RMCSKTAEDFQEPVSKDSLLIPH ***VKCLLL*LWYIIHSS*NIRPLA*IFHIYKM TDPTRKEAVAFFEVIFFLCSLLLKPYSFINNFA C*RKKKCFS*THYPGLFIAVGRTRSSLAPPVCKG SEDLIVQNTFCKCCAVNTANKLGSKHF

Sequence BRCA1.jAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACT TCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAG AACCAGGAGTGGAAAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGA CCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAG AGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTG TGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATG GCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTA CCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAG GCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTC CCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGC CACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTC AAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGA TTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGA AACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTG GCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGA GAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAA AAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGA ACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGA CCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGC TGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGAC TAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTT GGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKSSIN FTEK**IPYKPESRRPFC*QV*GVCR*FYQ*K*R TRSGKMLSLCVNGH*NIF*ELREENG*LAISG* PSLLKKEKC*MSMILKSEEMWSMEETTKVQSEQ ENPRTERSSGG*KSVAMGPSPTCPQINWNGWYSC VVLLW*RSFHHSPLAQVSTQLWLCSQMPGQRTM ASMQLGRCVRHLW*PESGCWTV*HSTSARSWTP T*YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSR PWELLSLFSPSTVLATKYFMYISLKRTSGYARV P*RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*T PPIEQDADSLFISPILSIQAVVGLGLEAQSGLAS RE*LVSLSLLL*NPVFTKAESQTLQWKESAWDR LCDLKSE*SLGSSQMLEWNIGEEILRQVLEMKR KLETWAWWLTPVIPALWEAKVGRSLEVRSSKPAW PTW*NPISTKNTEISRSWWWTPVIPATQVAKAG ESLQPGRWRLQ*AKIIPRHSSLGDSETVAQKKK KKKENETRRDF*KSEIYLLDF*RMCSKTAEDFQE PVSKDSLLIPH***VKCLLL*LWYIIHSS*NIR PLA*IFHIYKMTDPTRKEAVAFFEVIFFLCSLL LKPYSFINNFAC*RKKKCFS*THYPGLFIAVGRT RSSLAPPVCKGSEDLIVQNTFCKCCAVNTANKL GSKHF

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKAVLT SQKSSEYPISQNPEGLSADKFEVSADSSTSKNK EPGVERC*VCV*TDTEIFSRNCGRKMGS*LFLGD PVY*RKKNAE*A*F*SQRRCGQWKKPPRSKASK RIPGQKDLQGARNLLLWALHQHAHRSTGMDGTA VWCFCGEGAFIIHPWHRCPPNCGCAARCLDRGQW LPCNWADV*GTCGDPRVGVGQCSTLPVPGAGHL PDTPDPPQPLLTAASHRYRATGPQE*AYKVAFP GPGSSSHSSVLLLSWLLNILCTSA*KGLLAMQGS LKDFLLEVSLGNLP*AQNYGNFSPEKILKPFKR HQLSKMLIHYLSALFFLFRLLLA*GWKHRVAWP QENSWFP*VYFSKTLCSQRQRVRPFNGRRVLGID YVT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KG NLKPGHGGSRL*SQHFGRPRWADHWRSGVRNQP GQHGETPSLLKIQKLAGHGGGHL*SQLLRWLRQE NHFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKK KKRKMKLEEISKSLRYIC*ISKECVLKQQKIFK NRFPKTVF*FLISNK*NVYCCSSGI*SIPLKI*D LWHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC *NHTAS*IILLAEGRKSVFHKPIIQDCL*LLEG LGLP*PPQCARAVKT*LYKIRFVNVVLLTLQINL VANTS

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKQY*L HRKVVNTL*ARIQKAFLLTSLRCLQIVLPVKIK NQEWKDAEFVCERTLKYFLGIAGGKWVVSYFWV TQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRAR ESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQL CGASVVKELSSFTLGTGVHPIVVVQPDAWTEDN GFHAIGQMCEAPVVTREWVLDSVALYQCQELDTY LIPQIPHSHY*LQPATGTEPQDPKNELTKWPFQ ALGAPLTLQSFYCPGY*IFYVHQPEKDFWLCKG PLKIFCLKSPLEICHEHKIMVIFHLRRF*NHLNA TN*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGL KRIAGFPKFTSLKPCVHKGRESDPSMEGECLGS IM*LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKE T*NLGMVAHACNPSTLGGQGGQITGGQEFETSL ANMVKPHLY*KYRN*PVMVVDTCNPSYSGG*GR RITSAREVEVAVSQDHTTALQPG*Q*DCGSKKKK KKGK*N*KRFLKV*DIFARFLKNVF*NSRRFSR TGFQRQSSNSSLVISKMFIVVALVYNPFLLKYK TSGMNISYL*NDRSHQEGSCCFL*GDFFPLLPVA ETIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD *VFPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*T W*QTLP

Sequence BRCA1.iAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGGGGTGACCCAG TCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAAT CCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGG TGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTC CATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGA TACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCT GGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTA AAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCA ATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAG AATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATG TGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTT GAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAA CATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATC ACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAA AAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGG TTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCT GGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAA CCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGT CTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAG CAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKRGDP VY*RKKNAE*A*F*SQRRCGQWKKPPRSKASKRI PGQKDLQGARNLLLWALHQHAHRSTGMDGTAVW CFCGEGAFIIHPWHRCPPNCGCAARCLDRGQWL PCNWADV*GTCGDPRVGVGQCSTLPVPGAGHLPD TPDPPQPLLTAASHRYRATGPQE*AYKVAFPGP GSSSHSSVLLLSWLLNILCTSA*KGLLAMQGSL KDFLLEVSLGNLP*AQNYGNFSPEKILKPFKRHQ LSKMLIHYLSALFFLFRLLLA*GWKHRVAWPQE NSWFP*VYFSKTLCSQRQRVRPFNGRRVLGIDY VT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KGNL KPGHGGSRL*SQHFGRPRWADHWRSGVRNQPGQ HGETPSLLKIQKLAGHGGGHL*SQLLRWLRQEN HFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKKKK RKMKLEEISKSLRYIC*ISKECVLKQQKIFKNR FPKTVF*FLISNK*NVYCCSSGI*SIPLKI*DL WHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC*N HTAS*IILLAEGRKSVFHKPIIQDCL*LLEGLG LP*PPQCARAVKT*LYKIRFVNVVLLTLQINLV ANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKGVTQ SIKERKMLNEHDFEVRGDVVNGRNHQGPKRARE SQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCG ASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGF HAIGQMCEAPVVTREWVLDSVALYQCQELDTYL IPQIPHSHY*LQPATGTEPQDPKNELTKWPFQAL GAPLTLQSFYCPGY*IFYVHQPEKDFWLCKGPL KIFCLKSPLEICHEHKIMVIFHLRRF*NHLNAT N*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLKR IAGFPKFTSLKPCVHKGRESDPSMEGECLGSIM *LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKET *NLGMVAHACNPSTLGGQGGQITGGQEFETSLAN MVKPHLY*KYRN*PVMVVDTCNPSYSGG*GRRI TSAREVEVAVSQDHTTALQPG*Q*DCGSKKKKK KGK*N*KRFLKV*DIFARFLKNVF*NSRRFSRTG FQRQSSNSSLVISKMFIVVALVYNPFLLKYKTS GMNISYL*NDRSHQEGSCCFL*GDFFPLLPVAE TIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD*V FPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW* QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKG*PS LLKKEKC*MSMILKSEEMWSMEETTKVQSEQEN PRTERSSGG*KSVAMGPSPTCPQINWNGWYSCV VLLW*RSFHHSPLAQVSTQLWLCSQMPGQRTMAS MQLGRCVRHLW*PESGCWTV*HSTSARSWTPT* YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSRP WELLSLFSPSTVLATKYFMYISLKRTSGYARVP* RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*TPP IEQDADSLFISPILSIQAVVGLGLEAQSGLASR E*LVSLSLLL*NPVFTKAESQTLQWKESAWDRLC DLKSE*SLGSSQMLEWNIGEEILRQVLEMKRKL ETWAWWLTPVIPALWEAKVGRSLEVRSSKPAWP TW*NPISTKNTEISRSWWWTPVIPATQVAKAGES LQPGRWRLQ*AKIIPRHSSLGDSETVAQKKKKK KENETRRDF*KSEIYLLDF*RMCSKTAEDFQEP VSKDSLLIPH***VKCLLL*LWYIIHSS*NIRPL A*IFHIYKMTDPTRKEAVAFFEVIFFLCSLLLK PYSFINNFAC*RKKKCFS*THYPGLFIAVGRTR SSLAPPVCKGSEDLIVQNTFCKCCAVNTANKLGS KHF

Sequence BRCA1.hAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGATGCTGAGTTT GTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGC TGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAG GGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTT TCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTG AGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCA CTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAG TCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTC CCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCAT TATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTT ACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCC TTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCA CGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTAC TAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGG TTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAG ATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAAT TCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTAT AAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATT TTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTG CAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKRC*V CV*TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNA E*A*F*SQRRCGQWKKPPRSKASKRIPGQKDLQ GARNLLLWALHQHAHRSTGMDGTAVWCFCGEGA FIIHPWHRCPPNCGCAARCLDRGQWLPCNWADV* GTCGDPRVGVGQCSTLPVPGAGHLPDTPDPPQP LLTAASHRYRATGPQE*AYKVAFPGPGSSSHSS VLLLSWLLNILCTSA*KGLLAMQGSLKDFLLEVS LGNLP*AQNYGNFSPEKILKPFKRHQLSKMLIH YLSALFFLFRLLLA*GWKHRVAWPQENSWFP*V YFSKTLCSQRQRVRPFNGRRVLGIDYVT*SQNSP WAVLKCWSGTLGRKF*GRY*K*KGNLKPGHGGS RL*SQHFGRPRWADHWRSGVRNQPGQHGETPSL LKIQKLAGHGGGHL*SQLLRWLRQENHFSPGGGG CSEPRSYHGTPAWVTVRLWLKKKKKKRKMKLEE ISKSLRYIC*ISKECVLKQQKIFKNRFPKTVF* FLISNK*NVYCCSSGI*SIPLKI*DLWHEYFISI K*QIPPGRKLLLSLR*FFSFAPCC*NHTAS*II LLAEGRKSVFHKPIIQDCL*LLEGLGLP*PPQC ARAVKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKDAEF VCERTLKYFLGIAGGKWVVSYFWVTQSIKERKM LNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFR GLEICCYGPFTNMPTDQLEWMVQLCGASVVKEL SSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMC EAPVVTREWVLDSVALYQCQELDTYLIPQIPHSH Y*LQPATGTEPQDPKNELTKWPFQALGAPLTLQ SFYCPGY*IFYVHQPEKDFWLCKGPLKIFCLKS PLEICHEHKIMVIFHLRRF*NHLNATN*ARC*FI IYQPYSFYSGCCWLRAGSTEWLGLKRIAGFPKF TSLKPCVHKGRESDPSMEGECLGSIM*LKVRIV LGQFSNVGVEHWGGNSEAGIRNEKET*NLGMVAH ACNPSTLGGQGGQITGGQEFETSLANMVKPHLY *KYRN*PVMVVDTCNPSYSGG*GRRITSAREVE VAVSQDHTTALQPG*Q*DCGSKKKKKKGK*N*KR FLKV*DIFARFLKNVF*NSRRFSRTGFQRQSSN SSLVISKMFIVVALVYNPFLLKYKTSGMNISYL *NDRSHQEGSCCFL*GDFFPLLPVAETIQLHK*F CLLKEEKVFFINPLSRTVYSCWKD*VFPSPPSV QGQ*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKMLSL CVNGH*NIF*ELREENG*LAISG*PSLLKKEKC *MSMILKSEEMWSMEETTKVQSEQENPRTERSS GG*KSVAMGPSPTCPQINWNGWYSCVVLLW*RSF HHSPLAQVSTQLWLCSQMPGQRTMASMQLGRCV RHLW*PESGCWTV*HSTSARSWTPT*YPRSPTA TTDCSQPQVQSHRTPRMSLQSGLSRPWELLSLFS PSTVLATKYFMYISLKRTSGYARVP*RFSA*SL PWKSAMSTKLW*FFT*EDFKTI*TPPIEQDADS LFISPILSIQAVVGLGLEAQSGLASRE*LVSLSL LL*NPVFTKAESQTLQWKESAWDRLCDLKSE*S LGSSQMLEWNIGEEILRQVLEMKRKLETWAWWL TPVIPALWEAKVGRSLEVRSSKPAWPTW*NPIST KNTEISRSWWWTPVIPATQVAKAGESLQPGRWR LQ*AKIIPRHSSLGDSETVAQKKKKKKENETRR DF*KSEIYLLDF*RMCSKTAEDFQEPVSKDSLLI PH***VKCLLL*LWYIIHSS*NIRPLA*IFHIY KMTDPTRKEAVAFFEVIFFLCSLLLKPYSFINN FAC*RKKKCFS*THYPGLFIAVGRTRSSLAPPVC KGSEDLIVQNTFCKCCAVNTANKLGSKHF

Sequence BRCA1.fAug05

GAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCTGCTCTGGGTAAAGGTAGTAGAG TCCCGGGAAAGGGACAGGGGGCCCAAGTGATGCTCTGGGGTACTGGCGTGGGAGAGTGGATTTCCGAAGCTGACAGATGGTTCATTGGAACAGAAAGAAA TGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCAAGGAACC TGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGAGCCTACAAG AAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGC AAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAA CCCGAAAATCCTTCCTTGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGA CGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCC TCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGT AATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCA CAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAA ACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTG AATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAA ATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGT AGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGT AAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCC ATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTAC ATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAAT GGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAA AAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAA TAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGAT AGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAA CTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTT TACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAAT AATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTG ATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGA AAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAA ACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAG GAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAA TCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCC AAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAA ACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTC ACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGC AATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACA GAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAA AAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCA TCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTG TTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATT AGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACT AGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATAT TGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAA TACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCA GATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAA CAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCA GGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTG CGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACA AGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTA CATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCT GGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTG AATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGA ATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCA ACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTT TAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGG AGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAAT GGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGC CCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGT TGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTA GCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACC CCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCA GCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTT CACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGC TTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGA CCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGA AATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGA TCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTG TAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGG TGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGA ATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCT GGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTT TGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCAT TATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTA AATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

ETSWTGDRLWGFSDNWAPALRRPSPSALGKGSR VPGKGQGAQVMLWGTGVGEWISEADRWFIGTERN GFICSSR*RSTKCH*CYAENLRVSHLSGVDQGT CLHKV*PHILQILHAETSQPEERAFTVSFMSLQ ESTRFSQLVEELLKIICAFQLDTGLEYANSYNFA KKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSE PENPSLETSLSVQLSNLGTVRTLRTKQRIQPQK TSVYIELGSDSSEDTVNKATYCSVGDQELLQITP QGTRDEISLDSAKKAACEFSETDVTNTEHHQPS NNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCG TNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSK QPGLARSQHNRWAGSKETCNDRRTPSTEKKVDL NADPLCERKEWNKQKLPCSENPRDTEDVPWITL NSSIQKVNEWFSRSDELLGSDDSHDGESESNAKV ADVLDVLNEVDEYSGSSEKIDLLASDPHEALIC KSERVHSKSVESNIEDKIFGKTYRKKASLPNLS HVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPT SGLHPEDFIKKADLAVQKTPEMINQGTNQTEQN GQVMNITNSGHENKTKGDSIQNEKNPNPIESLE KESAFKTKAEPISSSISNMELELNIHNSKAPKKN RLRRKSSTRHIHALELVVSRNLSPPNCTELQID SCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPA TGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSF TKCSNTSELKEFVNPSLPREEKEEKLETVKVSN NAEDPKDLMLSGERVLQTERSVESSSISLVPGT DYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFE NPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRE TSIEMEESELDAQYLQNTFKVSKRQSFAPFSNP GNAEEECATFSAHSGSLKKQSPKVTFECEQKEEN QGKNESNIKPVQTVNITAGFPVVGQKDKPVDNA KCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQ NPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMS PEREMGNENIPSTVSTISRNNIRENVFKEASSS NINEVGSSTNEVGSSINEIGSSDENIQAELGRN RGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIK KQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHA SQVCSETPDDLLDDGEIKEDTSFAENDIKESSA VFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKL ESSEENLSSEDEELPCFQHLLFGKVNNIPSQST RHSTVATECLSKNTEENLLSLKNSLNDCSNQVI LAKASQEHHLSEETKCSASLFSSQCSELEDLTAN TNTQDPFLIGSSKQMRHQSESQGVGLSDKELVS DDEERGTGLEENNQEEQSMDSNLGEAASGCESE TSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQ EMAELEAVLEQHGSQPSNSYPSIISDSSALEDL RNPEQSTSEKAVLTSQKSSEYPISQNPEGLSAD KFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWY MHSCSGSLQNRNYPSQEELIKVVDVEEQQLEES GPHDLTETSYLPRQDLEGTPYLESGISLFSDDP ESDPSEDRAPESARVGNIPSSTSALKVPQLKVAE SAQSPAAAHTTDTAGYNAMEESVSREKPELTAS TERVNKRMSMVVSGLTPEEFMLVYKFARKHHIT LTNLITEETTHVVMKTDAEFVCERTLKYFLGIAG GKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVN GRNHQGPKRARESQDRKIFRGLEICCYGPFTNM PTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVV VQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSV ALYQCQELDTYLIPQIPHSHY*LQPATGTEPQD PKNELTKWPFQALGAPLTLQSFYCPGY*IFYVHQ PEKDFWLCKGPLKIFCLKSPLEICHEHKIMVIF HLRRF*NHLNATN*ARC*FIIYQPYSFYSGCCW LRAGSTEWLGLKRIAGFPKFTSLKPCVHKGRESD PSMEGECLGSIM*LKVRIVLGQFSNVGVEHWGG NSEAGIRNEKET*NLGMVAHACNPSTLGGQGGQ ITGGQEFETSLANMVKPHLY*KYRN*PVMVVDTC NPSYSGG*GRRITSAREVEVAVSQDHTTALQPG *Q*DCGSKKKKKKGK*N*KRFLKV*DIFARFLK NVF*NSRRFSRTGFQRQSSNSSLVISKMFIVVAL VYNPFLLKYKTSGMNISYL*NDRSHQEGSCCFL *GDFFPLLPVAETIQLHK*FCLLKEEKVFFINP LSRTVYSCWKD*VFPSPPSVQGQ*RLDCTKYVL* MLCC*HCK*TW*QTLP

Frame 1

RLPGRGTGCGVSQITGPLRSGGLHPLLWVKVVE SRERDRGPK*CSGVLAWESGFPKLTDGSLEQKE MDLSALRVEEVQNVINAMQKILECPICLELIKEP VSTKCDHIFCKFCMLKLLNQKKGPSQCPL*AYK KVRDLVNLLKSY*KSFVLFSLTQVWSMQTAIIL QKRKITLLNI*KMKFLSSKVWATETVPKDFYRVN PKILPWKPVSVSNSLTLEL*EL*GQSSGYNLKR RLSTLNWDLILLKIPLIRQLIAVWEIKNCYKSP LKEPGMKSVWILQKRLLVNFLRRM*QILNIINPV IMI*TPLRSVQLRGIQKSIRVVLFQTCMWSHVA QILMPAHYSMRTAVYYSLKTE*M*KRLNSVIKA NSLA*QGANITDGLEVRKHVMIGGLPAQKKR*I* MLIPCVREKNGISRNCHAQRILEILKMFLG*H* IAAFRKLMSGFPEVMNC*VLMTHMMGSLNQMPK *LMYWTF*MR*MNILVLQRK*TYWPVILMRL*YV KVKEFTPNQ*RVILKTKYLGKPIGRRQASPT*A M*LKI*L*EHLLLSHR*YKSVPSQIN*SVKGDL HQAFILRILSRKQIWQFKRLLK**IRELTKRSRM VK**ILLIVVMRIKQKVILFRMRKILTQ*NHSK KNLLSKRKLNL*AAV*AIWNSN*ISTIQKHLKR IG*GGSLLPGIFMRLN**SVEI*AHLIVLNCKLI VVLAVKR*RKKSTTKCQSGTAETYNSWKVKNLQ LEPRRVTSQMNRQVKDMTAILSQS*S*QMHLVL LLSVQIPVNLKNLSILAFQEKKKKRN*KQLKCLI MLKTPKISC*VEKGFCKLKDL*RVAVFHWYLVL IMALRKVSRYWKLAL*GRQKQNQINV*VSVQHL KTPRD*FMVVPKIIEMTQKALSIHWDMKLTTVGK QA*KWKKVNLMLSICRIHSRFQSASHLLRFQIQ EMQKRNVQHSLPTLGP*RNKVQKSLLNVNKRKK IKERMSLISSLYRQLISLQAFLWLVRKISQLIMP NVVSKEALGFVYHLSSEATKLDSLLQINMDFYK THIVYHHFFPSSHLLKLNVRKIC*RKTLRNIQC HLKEKWEMRTFQVQ*AQLAVITLEKMFLKKPAQA ILMK*VPVLMKWAPVLMK*VPVMKTFKQN*VET EGQN*MLCLD*GFCNLRSINKVFLEVIVSILK* KSKNMKK*FRLLIQISLHI*FQIT*NSLWEVVMH LRFVLRHLMTC*MMVK*RKILVLLKMTLRKVLL FLAKASRKESLAGVLALSPIHIWLRVTEEGPRN *SPQKRTYLVRMKSFPASNTCYLVK*TIYLLSLL GIAPLLPSVCLRTQRRIYYH*RIA*MTAVTR*Y WQRHLRNITLVRKQNVLLACFLHSAVNWKT*LQ IQTPRILS*LVLPNK*GISLKARELV*VTRNWFQ MMKKEERAWKKIIKKSKAWIQT*VKQHLGVRVK QASLKTAQGYPLRVTF*PLSRGIPCNIT**SSS RKWLN*KLC*NSMGASLLTATLPS*VTLLPLRTC EIQNKAHQKKQY*LHRKVVNTL*ARIQKAFLLT SLRCLQIVLPVKIKNQEWKGHPLLNAHH*MIGG TCTVALGVFRIETTHLKRSSLRLLMWRSNSWKSL GHTI*RKHLTCQGKI*REPLTWNLESASSLMTL NLILLKTEPQSQLVLATYHLQPLH*KFPN*KLQ NLPRVQLLLILLILLGIMQWKKV*AGRSQN*QLQ QKGSTKECPWWCLA*PQKNLCSCTSLPENTTSL *LI*LLKRLLMLL*KQMLSLCVNGH*NIF*ELR EENG*LAISG*PSLLKKEKC*MSMILKSEEMWSM EETTKVQSEQENPRTERSSGG*KSVAMGPSPTC PQINWNGWYSCVVLLW*RSFHHSPLAQVSTQLW LCSQMPGQRTMASMQLGRCVRHLW*PESGCWTV* HSTSARSWTPT*YPRSPTATTDCSQPQVQSHRT PRMSLQSGLSRPWELLSLFSPSTVLATKYFMYI SLKRTSGYARVP*RFSA*SLPWKSAMSTKLW*FF T*EDFKTI*TPPIEQDADSLFISPILSIQAVVG LGLEAQSGLASRE*LVSLSLLL*NPVFTKAESQ TLQWKESAWDRLCDLKSE*SLGSSQMLEWNIGEE ILRQVLEMKRKLETWAWWLTPVIPALWEAKVGR SLEVRSSKPAWPTW*NPISTKNTEISRSWWWTP VIPATQVAKAGESLQPGRWRLQ*AKIIPRHSSLG DSETVAQKKKKKKENETRRDF*KSEIYLLDF*R MCSKTAEDFQEPVSKDSLLIPH***VKCLLL*L WYIIHSS*NIRPLA*IFHIYKMTDPTRKEAVAFF EVIFFLCSLLLKPYSFINNFAC*RKKKCFS*TH YPGLFIAVGRTRSSLAPPVCKGSEDLIVQNTFC KCCAVNTANKLGSKHF

Frame 2

DFLDGGQAVGFLR*LGPCAQEAFTLCSG*R**S PGKGTGGPSDALGYWRGRVDFRS*QMVHWNRKK WIYLLFALKKYKMSLMLCRKS*SVPSVWS*SRN LSPQSVTTYFANFAC*NFSTRRKGLHSVLYEPTR KYEI*STC*RAIENHLCFSA*HRFGVCKQL*FC KKGK*LS*TSKR*SFYHPKYGLQKPCQKTSTE* TRKSFLGNQSQCPTL*PWNCENSEDKAADTTSKD VCLH*IGI*FF*RYR**GNLLQCGRSRIVTNHP SRNQG*NQFGFCKKGCL*IF*DGCNKY*TSSTQ ***FEHH*EACS*EASRKVSG*FCFKLACGAMWH KYSCQLITA*EQQFITH*RQNECRKG*IL**KQ TAWLSKEPT*QMGWK*GNM***ADSQHRKKGRS EC*SPV*EKRME*AETAMLRES*RY*RCSLDNTK *QHSES**VVFQK**TVRF**LT*WGV*IKCQS S*CIGRSK*GR*IFWFFRENRLTGQ*SS*GFNM *K*KSSLQISRE*Y*RQNIWENLSEEGKPPQLKP CN*KSNYRSICY*ATDNTRASPHK*IKA*KETY IRPSS*GFYQESRFGSSKDS*NDKSGN*PNGAE WSSDEYY**WS*E*NKR*FYSE*EKS*PNRITRK RICFQNES*TYKQQYKQYGTRIKYPQFKST*KE *AEEEVFYQAYSCA*TSSQ*KSKPT*LY*IAN* *LF*Q*RDKEKKVQPNASQAQQKPTTHGR*RTCN WSQEE*QAK*TDK*KT*QRYFPRAEVNKCTWFF Y*VFKYQ*T*RICQS*PSKRRKRRETRNS*SV* *C*RPQRSHVKWRKGFAN*KICRE*QYFIGTWY* LWHSGKYLVTGS*HSREGKNRTK*MCESVCSI* KPQGTNSWLFQR**K*HRRL*VSIGT*S*PQSG NKHRNGRK*T*CSVFAEYIQGFKAPVICSVFKSR KCRRGMCNILCPLWVLKETKSKSHF*M*TKGRK SRKE*V*YQACTDS*YHCRLSCGWSER*AS**C QM*YQRRL*VLSIISVQRQRNWTHYSK*TWTFTK PISYTTTFSHQVIC*N*M*EKSARGKL*GTFNV T*KRNGK*EHSKYSEHN*P**H*RKCF*RSQLK QY**SRFQY**SGLQY**NRFQ**KHSSRTR*KQ RAKIECYA*IRGFAT*GL*TKSSWK*L*AS*NK KARI*RSSSDC*YRFLSISDFR*LRTAYGK*SC ISGLF*DT**PVR*W*NKGRY*FC*K*H*GKFCC F*QKRPERRA*QES*PFHPYTFGSGLPKRGQEI RVLRRELI**G*RASLLPTLVIW*SKQYTFSVY *A*HRCYRVSV*EHRGEFIIIEE*LK*LQ*PGNI GKGISGTSP**GNKMFC*LVFFTVQ*IGRLDCK YKHPGSFLDWFFQTNEASV*KPGSWSE*QGIGF R**RKRNGLGRK*SRRAKHGFKLR*SSIWV*E*N KRL*RLLRAILSE*HFNHSAEGYHAT*PDKAPA GNG*TRSCVRTAWEPAF*QLPFHHK*LFCP*GP AKSRTKHIRKSSINFTEK**IPYKPESRRPFC*Q V*GVCR*FYQ*K*RTRSGKVIPF*MPIIR**VV HAQLLWESSE*KLPISRGAH*GC*CGGATAGRV WATRFDGNILLAKARSRGNPLPGIWNQPLL**P* I*SF*RQSPRVSSCWQHTIFNLCIESSPIESCR ICPESSCCSYY*YCWV*CNGRKCEQGEARIDSF NRKGQQKNVHGGVWPDPRRIYARVQVCQKTPHHF N*SNY*RDYSCCYENRC*VCV*TDTEIFSRNCG RKMGS*LFLGDPVY*RKKNAE*A*F*SQRRCGQ WKKPPRSKASKRIPGQKDLQGARNLLLWALHQHA HRSTGMDGTAVWCFCGEGAFIIHPWHRCPPNCG CAARCLDRGQWLPCNWADV*GTCGDPRVGVGQC STLPVPGAGHLPDTPDPPQPLLTAASHRYRATGP QE*AYKVAFPGPGSSSHSSVLLLSWLLNILCTS A*KGLLAMQGSLKDFLLEVSLGNLP*AQNYGNF SPEKILKPFKRHQLSKMLIHYLSALFFLFRLLLA *GWKHRVAWPQENSWFP*VYFSKTLCSQRQRVR PFNGRRVLGIDYVT*SQNSPWAVLKCWSGTLGR KF*GRY*K*KGNLKPGHGGSRL*SQHFGRPRWAD HWRSGVRNQPGQHGETPSLLKIQKLAGHGGGHL *SQLLRWLRQENHFSPGGGGCSEPRSYHGTPAW VTVRLWLKKKKKKRKMKLEEISKSLRYIC*ISKE CVLKQQKIFKNRFPKTVF*FLISNK*NVYCCSS GI*SIPLKI*DLWHEYFISIK*QIPPGRKLLLS LR*FFSFAPCC*NHTAS*IILLAEGRKSVFHKPI IQDCL*LLEGLGLP*PPQCARAVKT*LYKIRFV NVVLLTLQINLVANTS

Sequence BRCA1.eAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAGGTCTTACTCTGTTGTCCCAGCTGGAGTACAGTG GTGCGATCATGAGGCTTACTGTTGCCTTGACCTCCTAGGCTCAAGCGATCCTATCACCTCAGTCTCCCAAGTAGCTGGGACTATTTTGCATGCTGAAACT TCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAA GAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATC TAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAG TCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCT GATTCTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTT TGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAA GCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAG CATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAAC ATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAA AGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAG TGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATG AGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATC AGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGA GCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTA TCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAG TGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCT GAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCA GGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAA GAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCA AATGAACAGACAAGTAAAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAAC TTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCAT GTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCG TTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTT GTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGA ACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACA TTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCA AGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAG GTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTT CCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGA ACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTAC TAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTT AGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTC AGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGA TGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGA GAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTA GTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTG TCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCAC CTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGA TTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTT GGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGG CTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGT TAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGA AAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCT ACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTC AGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATC TTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCC CCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTC ATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTC CATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACT ACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCT GGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCG AGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTA CAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGG ACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGA CACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCT TTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCA AGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTT AAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTT GGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGG GATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGA AAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACC AGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAG GCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAA AAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTT TCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAAT ATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCC TGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGG AAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAAT AAACTTGGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQGLTLLSQLEYS GAIMRLTVALTS*AQAILSPQSPK*LGLFCMLKL LNQKKGPSQCPLCKNDITKRSLQESTRFSQLVE ELLKIICAFQLDTGLEYANSYNFAKKENNSPEH LKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETS LSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGS DSSEDTVNKATYCSVGDQELLQITPQGTRDEIS LDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEK RAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQ HENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQ HNRWAGSKETCNDRRTPSTEKKVDLNADPLCERK EWNKQKLPCSENPRDTEDVPWITLNSSIQKVNE WFSRSDELLGSDDSHDGESESNAKVADVLDVLN EVDEYSGSSEKIDLLASDPHEALICKSERVHSKS VESNIEDKIFGKTYRKKASLPNLSHVTENLIIG AFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDF IKKADLAVQKTPEMINQGTNQTEQNGQVMNITNS GHENKTKGDSIQNEKNPNPIESLEKESAFKTKA EPISSSISNMELELNIHNSKAPKKNRLRRKSST RHIHALELVVSRNLSPPNCTELQIDSCSSSEEIK KKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKP NEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSE LKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLM LSGERVLQTERSVESSSISLVPGTDYGTQESIS LLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHG CSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESE LDAQYLQNTFKVSKRQSFAPFSNPGNAEEECAT FSAHSGSLKKQSPKVTFECEQKEENQGKNESNI KPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSR FCLSSQFRGNETGLITPNKHGLLQNPYRIPPLF PIKSFVKTKCKKNLLEENFEEHSMSPEREMGNE NIPSTVSTISRNNIRENVFKEASSSNINEVGSST NEVGSSINEIGSSDENIQAELGRNRGPKLNAML RLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVV QTVNTDFSPYLISDNLEQPMGSSHASQVCSETPD DLLDDGEIKEDTSFAENDIKESSAVFSKSVQKG ELSRSPSPFTHTHLAQGYRRGAKKLESSEENLS SEDEELPCFQHLLFGKVNNIPSQSTRHSTVATEC LSKNTEENLLSLKNSLNDCSNQVILAKASQEHH LSEETKCSASLFSSQCSELEDLTANTNTQDPFL IGSSKQMRHQSESQGVGLSDKELVSDDEERGTGL EENNQEEQSMDSNLGEAASGCESETSVSEDCSG LSSQSDILTTQQRDTMQHNLIKLQQEMAELEAV LEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSE KAVLTSQKSSEYPISQNPEGLSADKFEVSADSS TSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSL QNRNYPSQEELIKVVDVEEQQLEESGPHDLTETS YLPRQDLEGTPYLESGISLFSDDPESDPSEDRA PESARVGNIPSSTSALKVPQLKVAESAQSPAAA HTTDTAGYNAMEESVSREKPELTASTERVNKRMS MVVSGLTPEEFMLVYKFARKHHITLTNLITEET THVVMKTDAEFVCERTLKYFLGIAGGKWVVSYF WVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKR ARESQDRKIFRGLEICCYGPFTNMPTDQLEWMV QLCGASVVKELSSFTLGTGVHPIVVVQPDAWTE DNGFHAIGQMCEAPVVTREWVLDSVALYQCQELD TYLIPQIPHSHY*LQPATGTEPQDPKNELTKWP FQALGAPLTLQSFYCPGY*IFYVHQPEKDFWLC KGPLKIFCLKSPLEICHEHKIMVIFHLRRF*NHL NATN*ARC*FIIYQPYSFYSGCCWLRAGSTEWL GLKRIAGFPKFTSLKPCVHKGRESDPSMEGECL GSIM*LKVRIVLGQFSNVGVEHWGGNSEAGIRNE KET*NLGMVAHACNPSTLGGQGGQITGGQEFET SLANMVKPHLY*KYRN*PVMVVDTCNPSYSGG* GRRITSAREVEVAVSQDHTTALQPG*Q*DCGSKK KKKKGK*N*KRFLKV*DIFARFLKNVF*NSRRF SRTGFQRQSSNSSLVISKMFIVVALVYNPFLLK YKTSGMNISYL*NDRSHQEGSCCFL*GDFFPLLP VAETIQLHK*FCLLKEEKVFFINPLSRTVYSCW KD*VFPSPPSVQGQ*RLDCTKYVL*MLCC*HCK *TW*QTLP

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKVLLCCPSWSTV VRS*GLLLP*PPRLKRSYHLSLPSSWDYFAC*N FSTRRKGLHSVLYVRMI*PKGAYKKVRDLVNLLK SY*KSFVLFSLTQVWSMQTAIILQKRKITLLNI *KMKFLSSKVWATETVPKDFYRVNPKILPCRKP VSVSNSLTLEL*EL*GQSSGYNLKRRLSTLNWDL ILLKIPLIRQLIAVWEIKNCYKSPLKEPGMKSV WILQKRLLVNFLRRM*QILNIINPVIMI*TPLR SVQLRGIQKSIRVVLFQTCMWSHVAQILMPAHYS MRTAVYYSLKTE*M*KRLNSVIKANSLA*QGAN ITDGLEVRKHVMIGGLPAQKKR*I*MLIPCVRE KNGISRNCHAQRILEILKMFLG*H*IAAFRKLMS GFPEVMNC*VLMTHMMGSLNQMPK*LMYWTF*M R*MNILVLQRK*TYWPVILMRL*YVKVKEFTPN Q*RVILKTKYLGKPIGRRQASPT*AM*LKI*L*E HLLLSHR*YKSVPSQIN*SVKGDLHQAFILRIL SRKQIWQFKRLLK**IRELTKRSRMVK**ILLI VVMRIKQKVILFRMRKILTQ*NHSKKNLLSKRKL NL*AAV*AIWNSN*ISTIQKHLKRIG*GGSLLP GIFMRLN**SVEI*AHLIVLNCKLIVVLAVKR* RKKSTTKCQSGTAETYNSWKVKNLQLEPRRVTSQ MNRQVKDMTAILSQS*S*QMHLVLLLSVQIPVN LKNLSILAFQEKKKKRN*KQLKCLIMLKTPKIS C*VEKGFCKLKDL*RVAVFHWYLVLIMALRKVSR YWKLAL*GRQKQNQINV*VSVQHLKTPRD*FMV VPKIIEMTQKALSIHWDMKLTTVGKQA*KWKKV NLMLSICRIHSRFQSASHLLRFQIQEMQKRNVQH SLPTLGP*RNKVQKSLLNVNKRKKIKERMSLIS SLYRQLISLQAFLWLVRKISQLIMPNVVSKEAL GFVYHLSSEATKLDSLLQINMDFYKTHIVYHHFF PSSHLLKLNVRKIC*RKTLRNIQCHLKEKWEMR TFQVQ*AQLAVITLEKMFLKKPAQAILMK*VPV LMKWAPVLMK*VPVMKTFKQN*VETEGQN*MLCL D*GFCNLRSINKVFLEVIVSILK*KSKNMKK*F RLLIQISLHI*FQIT*NSLWEVVMHLRFVLRHL MTC*MMVK*RKILVLLKMTLRKVLLFLAKASRKE SLAGVLALSPIHIWLRVTEEGPRN*SPQKRTYL VRMKSFPASNTCYLVK*TIYLLSLLGIAPLLPS VCLRTQRRIYYH*RIA*MTAVTR*YWQRHLRNIT LVRKQNVLLACFLHSAVNWKT*LQIQTPRILS* LVLPNK*GISLKARELV*VTRNWFQMMKKEERA WKKIIKKSKAWIQT*VKQHLGVRVKQASLKTAQG YPLRVTF*PLSRGIPCNIT**SSSRKWLN*KLC *NSMGASLLTATLPS*VTLLPLRTCEIQNKAHQ KKQY*LHRKVVNTL*ARIQKAFLLTSLRCLQIVL PVKIKNQEWKGHPLLNAHH*MIGGTCTVALGVF RIETTHLKRSSLRLLMWRSNSWKSLGHTI*RKH LTCQGKI*REPLTWNLESASSLMTLNLILLKTEP QSQLVLATYHLQPLH*KFPN*KLQNLPRVQLLL ILLILLGIMQWKKV*AGRSQN*QLQQKGSTKEC PWWCLA*PQKNLCSCTSLPENTTSL*LI*LLKRL LMLL*KQMLSLCVNGH*NIF*ELREENG*LAIS G*PSLLKKEKC*MSMILKSEEMWSMEETTKVQS EQENPRTERSSGG*KSVAMGPSPTCPQINWNGWY SCVVLLW*RSFHHSPLAQVSTQLWLCSQMPGQR TMASMQLGRCVRHLW*PESGCWTV*HSTSARSW TPT*YPRSPTATTDCSQPQVQSHRTPRMSLQSGL SRPWELLSLFSPSTVLATKYFMYISLKRTSGYA RVP*RFSA*SLPWKSAMSTKLW*FFT*EDFKTI *TPPIEQDADSLFISPILSIQAVVGLGLEAQSGL ASRE*LVSLSLLL*NPVFTKAESQTLQWKESAW DRLCDLKSE*SLGSSQMLEWNIGEEILRQVLEM KRKLETWAWWLTPVIPALWEAKVGRSLEVRSSKP AWPTW*NPISTKNTEISRSWWWTPVIPATQVAK AGESLQPGRWRLQ*AKIIPRHSSLGDSETVAQK KKKKKENETRRDF*KSEIYLLDF*RMCSKTAEDF QEPVSKDSLLIPH***VKCLLL*LWYIIHSS*N IRPLA*IFHIYKMTDPTRKEAVAFFEVIFFLCS LLLKPYSFINNFAC*RKKKCFS*THYPGLFIAVG RTRSSLAPPVCKGSEDLIVQNTFCKCCAVNTAN KLGSKHF

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFARSYSVVPAGVQW CDHEAYCCLDLLGSSDPITSVSQVAGTILHAET SQPEERAFTVSFM*E*YNQKEPTRKYEI*STC* RAIENHLCFSA*HRFGVCKQL*FCKKGK*LS*TS KR*SFYHPKYGLQKPCQKTSTE*TRKSFLAGNQ SQCPTL*PWNCENSEDKAADTTSKDVCLH*IGI *FF*RYR**GNLLQCGRSRIVTNHPSRNQG*NQF GFCKKGCL*IF*DGCNKY*TSSTQ***FEHH*E ACS*EASRKVSG*FCFKLACGAMWHKYSCQLIT A*EQQFITH*RQNECRKG*IL**KQTAWLSKEPT *QMGWK*GNM***ADSQHRKKGRSEC*SPV*EK RME*AETAMLRES*RY*RCSLDNTK*QHSES** VVFQK**TVRF**LT*WGV*IKCQSS*CIGRSK* GR*IFWFFRENRLTGQ*SS*GFNM*K*KSSLQI SRE*Y*RQNIWENLSEEGKPPQLKPCN*KSNYR SICY*ATDNTRASPHK*IKA*KETYIRPSS*GFY QESRFGSSKDS*NDKSGN*PNGAEWSSDEYY** WS*E*NKR*FYSE*EKS*PNRITRKRICFQNES *TYKQQYKQYGTRIKYPQFKST*KE*AEEEVFYQ AYSCA*TSSQ*KSKPT*LY*IAN**LF*Q*RDK EKKVQPNASQAQQKPTTHGR*RTCNWSQEE*QA K*TDK*KT*QRYFPRAEVNKCTWFFY*VFKYQ*T *RICQS*PSKRRKRRETRNS*SV**C*RPQRSH VKWRKGFAN*KICRE*QYFIGTWY*LWHSGKYL VTGS*HSREGKNRTK*MCESVCSI*KPQGTNSWL FQR**K*HRRL*VSIGT*S*PQSGNKHRNGRK* T*CSVFAEYIQGFKAPVICSVFKSRKCRRGMCN ILCPLWVLKETKSKSHF*M*TKGRKSRKE*V*YQ ACTDS*YHCRLSCGWSER*AS**CQM*YQRRL* VLSIISVQRQRNWTHYSK*TWTFTKPISYTTTF SHQVIC*N*M*EKSARGKL*GTFNVT*KRNGK*E HSKYSEHN*P**H*RKCF*RSQLKQY**SRFQY **SGLQY**NRFQ**KHSSRTR*KQRAKIECYA *IRGFAT*GL*TKSSWK*L*AS*NKKARI*RSSS DC*YRFLSISDFR*LRTAYGK*SCISGLF*DT* *PVR*W*NKGRY*FC*K*H*GKFCCF*QKRPER RA*QES*PFHPYTFGSGLPKRGQEIRVLRRELI* *G*RASLLPTLVIW*SKQYTFSVY*A*HRCYRV SV*EHRGEFIIIEE*LK*LQ*PGNIGKGISGTS P**GNKMFC*LVFFTVQ*IGRLDCKYKHPGSFLD WFFQTNEASV*KPGSWSE*QGIGFR**RKRNGL GRK*SRRAKHGFKLR*SSIWV*E*NKRL*RLLR AILSE*HFNHSAEGYHAT*PDKAPAGNG*TRSCV RTAWEPAF*QLPFHHK*LFCP*GPAKSRTKHIR KSSINFTEK**IPYKPESRRPFC*QV*GVCR*F YQ*K*RTRSGKVIPF*MPIIR**VVHAQLLWESS E*KLPISRGAH*GC*CGGATAGRVWATRFDGNI LLAKARSRGNPLPGIWNQPLL**P*I*SF*RQS PRVSSCWQHTIFNLCIESSPIESCRICPESSCCS YY*YCWV*CNGRKCEQGEARIDSFNRKGQQKNV HGGVWPDPRRIYARVQVCQKTPHHFN*SNY*RD YSCCYENRC*VCV*TDTEIFSRNCGRKMGS*LFL GDPVY*RKKNAE*A*F*SQRRCGQWKKPPRSKA SKRIPGQKDLQGARNLLLWALHQHAHRSTGMDG TAVWCFCGEGAFIIHPWHRCPPNCGCAARCLDRG QWLPCNWADV*GTCGDPRVGVGQCSTLPVPGAG HLPDTPDPPQPLLTAASHRYRATGPQE*AYKVA FPGPGSSSHSSVLLLSWLLNILCTSA*KGLLAMQ GSLKDFLLEVSLGNLP*AQNYGNFSPEKILKPF KRHQLSKMLIHYLSALFFLFRLLLA*GWKHRVA WPQENSWFP*VYFSKTLCSQRQRVRPFNGRRVLG IDYVT*SQNSPWAVLKCWSGTLGRKF*GRY*K* KGNLKPGHGGSRL*SQHFGRPRWADHWRSGVRN QPGQHGETPSLLKIQKLAGHGGGHL*SQLLRWLR QENHFSPGGGGCSEPRSYHGTPAWVTVRLWLKK KKKKRKMKLEEISKSLRYIC*ISKECVLKQQKI FKNRFPKTVF*FLISNK*NVYCCSSGI*SIPLKI *DLWHEYFISIK*QIPPGRKLLLSLR*FFSFAP CC*NHTAS*IILLAEGRKSVFHKPIIQDCL*LL EGLGLP*PPQCARAVKT*LYKIRFVNVVLLTLQI NLVANTS

Sequence BRCA1.cAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC TCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG CATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTT TATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGG AAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAG AAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTG ATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTC TGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATT GAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGC CACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTT GGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAA ACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCA GTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCT TGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAAC CAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTA AAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAA TCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGG GTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCA CTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAG AAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTAT TTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTG GGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGT TAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCT CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTG TTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGT GAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCC AGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGC AACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGA TTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGAT GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTC CTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCT TCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACA GAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAA AATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACA AATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAA GAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTG ACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAG CCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACT TCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAG AACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCC ATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAA GATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTG TTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGC TGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGC CTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGA AAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTAT TAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAG GACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTT CTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGC AATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCC CAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAG CTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGAT TTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGA GCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAG CTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACT TAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAAC CTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGG TGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTC AGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGA AAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCC AAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCAT GAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATA CAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCC CTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAAC ACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT L*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E ASRKVSG*FCFKLACGAMWHKYSCQLITA*EQQF ITH*RQNECRKG*IL**KQTAWLSKEPT*QMGW K*GNM***ADSQHRKKGRSEC*SPV*EKRME*A ETAMLRES*RY*RCSLDNTK*QHSES**VVFQK* *TVRF**LT*WGV*IKCQSS*CIGRSK*GR*IF WFFRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y *RQNIWENLSEEGKPPQLKPCN*KSNYRSICY*A TDNTRASPHK*IKA*KETYIRPSS*GFYQESRF GSSKDS*NDKSGN*PNGAEWSSDEYY**WS*E* NKR*FYSE*EKS*PNRITRKRICFQNES*TYKQQ YKQYGTRIKYPQFKST*KE*AEEEVFYQAYSCA *TSSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQ PNASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK* KT*QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQ S*PSKRRKRRETRNS*SV**C*RPQRSHVKWRK GFAN*KICRE*QYFIGTWY*LWHSGKYLVTGS*H SREGKNRTK*MCESVCSI*KPQGTNSWLFQR** K*HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSV FAEYIQGFKAPVICSVFKSRKCRRGMCNILCPLW VLKETKSKSHF*M*TKGRKSRKE*V*YQACTDS *YHCRLSCGWSER*AS**CQM*YQRRL*VLSII SVQRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC *N*M*EKSARGKL*GTFNVT*KRNGK*EHSKYS EHN*P**H*RKCF*RSQLKQY**SRFQY**SGL QY**NRFQ**KHSSRTR*KQRAKIECYA*IRGFA T*GL*TKSSWK*L*AS*NKKARI*RSSSDC*YR FLSISDFR*LRTAYGK*SCISGLF*DT**PVR* W*NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES *PFHPYTFGSGLPKRGQEIRVLRRELI**G*RA SLLPTLVIW*SKQYTFSVY*A*HRCYRVSV*EH RGEFIIIEE*LK*LQ*PGNIGKGISGTSP**GNK MFC*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQT NEASV*KPGSWSE*QGIGFR**RKRNGLGRK*S RRAKHGFKLR*SSIWV*E*NKRL*RLLRAILSE* HFNHSAEGYHAT*PDKAPAGNG*TRSCVRTAWE PAF*QLPFHHK*LFCP*GPAKSRTKHIRKSSIN FTEK**IPYKPESRRPFC*QV*GVCR*FYQ*K*R TRSGKVIPF*MPIIR**VVHAQLLWESSE*KLP ISRGAH*GC*CGGATAGRVWATRFDGNILLAKA RSRGNPLPGIWNQPLL**P*I*SF*RQSPRVSSC WQHTIFNLCIESSPIESCRICPESSCCSYY*YC WV*CNGRKCEQGEARIDSFNRKGQQKNVHGGVW PDPRRIYARVQVCQKTPHHFN*SNY*RDYSCCYE NRC*VCV*TDTEIFSRNCGRKMGS*LFLGDPVY *RKKNAE*A*F*SQRRCGQWKKPPRSKASKRIP GQKDLQGARNLLLWALHQHAHRSTGMDGTAVWCF CGEGAFIIHPWHRCPPNCGCAARCLDRGQWLPC NWADV*GTCGDPRVGVGQCSTLPVPGAGHLPDT PDPPQPLLTAASHRYRATGPQE*AYKVAFPGPGS SSHSSVLLLSWLLNILCTSA*KGLLAMQGSLKD FLLEVSLGNLP*AQNYGNFSPEKILKPFKRHQL SKMLIHYLSALFFLFRLLLA*GWKHRVAWPQENS WFP*VYFSKTLCSQRQRVRPFNGRRVLGIDYVT *SQNSPWAVLKCWSGTLGRKF*GRY*K*KGNLK PGHGGSRL*SQHFGRPRWADHWRSGVRNQPGQHG ETPSLLKIQKLAGHGGGHL*SQLLRWLRQENHF SPGGGGCSEPRSYHGTPAWVTVRLWLKKKKKKR KMKLEEISKSLRYIC*ISKECVLKQQKIFKNRFP KTVF*FLISNK*NVYCCSSGI*SIPLKI*DLWH EYFISIK*QIPPGRKLLLSLR*FFSFAPCC*NH TAS*IILLAEGRKSVFHKPIIQDCL*LLEGLGLP *PPQCARAVKT*LYKIRFVNVVLLTLQINLVAN TS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSED TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSS LLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRS DELLGSDDSHDGESESNAKVADVLDVLNEVDEYS GSSEKIDLLASDPHEALICKSERVHSKSVESNI EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTE PQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADL AVQKTPEMINQGTNQTEQNGQVMNITNSGHENK TKGDSIQNEKNPNPIESLEKESAFKTKAEPISS SISNMELELNIHNSKAPKKNRLRRKSSTRHIHAL ELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTS KRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVN PSLPREEKEEKLETVKVSNNAEDPKDLMLSGER VLQTERSVESSSISLVPGTDYGTQESISLLEVS TLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNR NDTEGFKYPLGHEVNHSRETSIEMEESELDAQY LQNTFKVSKRQSFAPFSNPGNAEEECATFSAHS GSLKKQSPKVTFECEQKEENQGKNESNIKPVQTV NITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTV STISRNNIRENVFKEASSSNINEVGSSTNEVGS SINEIGSSDENIQAELGRNRGPKLNAMLRLGVL QPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTD FSPYLISDNLEQPMGSSHASQVCSETPDDLLDD GEIKEDTSFAENDIKESSAVFSKSVQKGELSRS PSPFTHTHLAQGYRRGAKKLESSEENLSSEDEEL PCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT EENLLSLKNSLNDCSNQVILAKASQEHHLSEET KCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQ MRHQSESQGVGLSDKELVSDDEERGTGLEENNQ EEQSMDSNLGEAASGCESETSVSEDCSGLSSQS DILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGS QPSNSYPSIISDSSALEDLRNPEQSTSEKAVLT SQKSSEYPISQNPEGLSADKFEVSADSSTSKNK EPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYP SQEELIKVVDVEEQQLEESGPHDLTETSYLPRQ DLEGTPYLESGISLFSDDPESDPSEDRAPESAR VGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTA GYNAMEESVSREKPELTASTERVNKRMSMVVSG LTPEEFMLVYKFARKHHITLTNLITEETTHVVM KTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSI KERKMLNEHDFEVRGDVVNGRNHQGPKRARESQ DRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGA SVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHA IGQMCEAPVVTREWVLDSVALYQCQELDTYLIP QIPHSHY*LQPATGTEPQDPKNELTKWPFQALG APLTLQSFYCPGY*IFYVHQPEKDFWLCKGPLKI FCLKSPLEICHEHKIMVIFHLRRF*NHLNATN* ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLKRI AGFPKFTSLKPCVHKGRESDPSMEGECLGSIM*L KVRIVLGQFSNVGVEHWGGNSEAGIRNEKET*N LGMVAHACNPSTLGGQGGQITGGQEFETSLANM VKPHLY*KYRN*PVMVVDTCNPSYSGG*GRRITS AREVEVAVSQDHTTALQPG*Q*DCGSKKKKKKG K*N*KRFLKV*DIFARFLKNVF*NSRRFSRTGF QRQSSNSSLVISKMFIVVALVYNPFLLKYKTSGM NISYL*NDRSHQEGSCCFL*GDFFPLLPVAETI QLHK*FCLLKEEKVFFINPLSRTVYSCWKD*VF PSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW*QT LP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS* SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS LTLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG IQKSIRVVLFQTCMWSHVAQILMPAHYSMRTAV YYSLKTE*M*KRLNSVIKANSLA*QGANITDGL EVRKHVMIGGLPAQKKR*I*MLIPCVREKNGISR NCHAQRILEILKMFLG*H*IAAFRKLMSGFPEV MNC*VLMTHMMGSLNQMPK*LMYWTF*MR*MNI LVLQRK*TYWPVILMRL*YVKVKEFTPNQ*RVIL KTKYLGKPIGRRQASPT*AM*LKI*L*EHLLLS HR*YKSVPSQIN*SVKGDLHQAFILRILSRKQI WQFKRLLK**IRELTKRSRMVK**ILLIVVMRIK QKVILFRMRKILTQ*NHSKKNLLSKRKLNL*AA V*AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMR LN**SVEI*AHLIVLNCKLIVVLAVKR*RKKSTT KCQSGTAETYNSWKVKNLQLEPRRVTSQMNRQV KDMTAILSQS*S*QMHLVLLLSVQIPVNLKNLS ILAFQEKKKKRN*KQLKCLIMLKTPKISC*VEKG FCKLKDL*RVAVFHWYLVLIMALRKVSRYWKLA L*GRQKQNQINV*VSVQHLKTPRD*FMVVPKII EMTQKALSIHWDMKLTTVGKQA*KWKKVNLMLSI CRIHSRFQSASHLLRFQIQEMQKRNVQHSLPTL GP*RNKVQKSLLNVNKRKKIKERMSLISSLYRQ LISLQAFLWLVRKISQLIMPNVVSKEALGFVYHL SSEATKLDSLLQINMDFYKTHIVYHHFFPSSHL LKLNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ *AQLAVITLEKMFLKKPAQAILMK*VPVLMKWAP VLMK*VPVMKTFKQN*VETEGQN*MLCLD*GFC NLRSINKVFLEVIVSILK*KSKNMKK*FRLLIQ ISLHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MM VK*RKILVLLKMTLRKVLLFLAKASRKESLAGV LALSPIHIWLRVTEEGPRN*SPQKRTYLVRMKS FPASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQ RRIYYH*RIA*MTAVTR*YWQRHLRNITLVRKQ NVLLACFLHSAVNWKT*LQIQTPRILS*LVLPN K*GISLKARELV*VTRNWFQMMKKEERAWKKIIK KSKAWIQT*VKQHLGVRVKQASLKTAQGYPLRV TF*PLSRGIPCNIT**SSSRKWLN*KLC*NSMG ASLLTATLPS*VTLLPLRTCEIQNKAHQKKQY*L HRKVVNTL*ARIQKAFLLTSLRCLQIVLPVKIK NQEWKGHPLLNAHH*MIGGTCTVALGVFRIETT HLKRSSLRLLMWRSNSWKSLGHTI*RKHLTCQGK I*REPLTWNLESASSLMTLNLILLKTEPQSQLV LATYHLQPLH*KFPN*KLQNLPRVQLLLILLIL LGIMQWKKV*AGRSQN*QLQQKGSTKECPWWCLA *PQKNLCSCTSLPENTTSL*LI*LLKRLLMLL* KQMLSLCVNGH*NIF*ELREENG*LAISG*PSL LKKEKC*MSMILKSEEMWSMEETTKVQSEQENPR TERSSGG*KSVAMGPSPTCPQINWNGWYSCVVL LW*RSFHHSPLAQVSTQLWLCSQMPGQRTMASM QLGRCVRHLW*PESGCWTV*HSTSARSWTPT*YP RSPTATTDCSQPQVQSHRTPRMSLQSGLSRPWE LLSLFSPSTVLATKYFMYISLKRTSGYARVP*R FSA*SLPWKSAMSTKLW*FFT*EDFKTI*TPPIE QDADSLFISPILSIQAVVGLGLEAQSGLASRE* LVSLSLLL*NPVFTKAESQTLQWKESAWDRLCD LKSE*SLGSSQMLEWNIGEEILRQVLEMKRKLET WAWWLTPVIPALWEAKVGRSLEVRSSKPAWPTW *NPISTKNTEISRSWWWTPVIPATQVAKAGESL QPGRWRLQ*AKIIPRHSSLGDSETVAQKKKKKKE NETRRDF*KSEIYLLDF*RMCSKTAEDFQEPVS KDSLLIPH***VKCLLL*LWYIIHSS*NIRPLA *IFHIYKMTDPTRKEAVAFFEVIFFLCSLLLKPY SFINNFAC*RKKKCFS*THYPGLFIAVGRTRSS LAPPVCKGSEDLIVQNTFCKCCAVNTANKLGSK HF

Sequence BRCA1.bAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT GCTCTGGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCC CATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCT TCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTG CTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTCTATCAT CCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTCTCTAAC CTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATACCGTTA ATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGC TTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCA GAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATGAGAACAGCAGTTTATTAC TCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAA GGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTG CCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAAC TGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAATATTCTGGTTC TTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTAGAGAGTAATATTGAAGAC AAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGCCACAGA TAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATTTGGCAGT TCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAAACAAAA GGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAACCTATAAGCAGCAGTATAA GCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCTTGAACT AGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATG CCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATGAACAGACAAGTAAAAGAC ATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAATCCTAG CCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTG CAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAG GGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAGAAATGA CACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTATTTGCAG AATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCACTCTGGGTCCT TAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCCTGTACAGACAGTTAATAT CACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTC AGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCATCAAGTCATTTGTTAAAA CTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGTGAGCAC AATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAATGAAGTGGGCTCCAGTATT AATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTG AGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATACAGATTTCTC TCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGACCTGTTAGATGATGGTGAA ATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTCCTAGCC CTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGAGGATGAAGAGCTTCCCTG CTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAG AATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTAGTGAGGAAACAAAATGTT CTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAG GCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAATCAAGAAGAG CAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTT TAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCC TTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAG AAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAG GAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCA AGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTA GAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCA ACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTA TAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACC CCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAG ATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGA AAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGA AAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGG TGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGG GCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATC CCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTC TCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTG CTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGA TGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTT TCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGT CAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGC ATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAAC CCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCG GGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGA AACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGAC AGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATAT TTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTT CATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCC CCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCC A

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL LWFIGTERNGFICSSR*RSTKCH*CYAENLRVS HLSGVDQGTCLHKV*PHILQILHAETSQPEERA FTVSFM*E*YNQKEPTRKYEI*STC*RAIENHLC FSA*HRFGVCKQL*FCKKGK*LS*TSKR*SFYH PKYGLQKPCQKTSTE*TRKSFLAGNQSQCPTL* PWNCENSEDKAADTTSKDVCLH*IGI*FF*RYR* *GNLLQCGRSRIVTNHPSRNQG*NQFGFCKKGC L*IF*DGCNKY*TSSTQ***FEHH*EACS*EAS RKVSG*FCFKLACGAMWHKYSCQLITA*EQQFIT H*RQNECRKG*IL**KQTAWLSKEPT*QMGWK* GNM***ADSQHRKKGRSEC*SPV*EKRME*AET AMLRES*RY*RCSLDNTK*QHSES**VVFQK**T VRF**LT*WGV*IKCQSS*CIGRSK*GR*IFWF FRENRLTGQ*SS*GFNM*K*KSSLQISRE*Y*R QNIWENLSEEGKPPQLKPCN*KSNYRSICY*ATD NTRASPHK*IKA*KETYIRPSS*GFYQESRFGS SKDS*NDKSGN*PNGAEWSSDEYY**WS*E*NK R*FYSE*EKS*PNRITRKRICFQNES*TYKQQYK QYGTRIKYPQFKST*KE*AEEEVFYQAYSCA*T SSQ*KSKPT*LY*IAN**LF*Q*RDKEKKVQPN ASQAQQKPTTHGR*RTCNWSQEE*QAK*TDK*KT *QRYFPRAEVNKCTWFFY*VFKYQ*T*RICQS* PSKRRKRRETRNS*SV**C*RPQRSHVKWRKGF AN*KICRE*QYFIGTWY*LWHSGKYLVTGS*HSR EGKNRTK*MCESVCSI*KPQGTNSWLFQR**K* HRRL*VSIGT*S*PQSGNKHRNGRK*T*CSVFA EYIQGFKAPVICSVFKSRKCRRGMCNILCPLWVL KETKSKSHF*M*TKGRKSRKE*V*YQACTDS*Y HCRLSCGWSER*AS**CQM*YQRRL*VLSIISV QRQRNWTHYSK*TWTFTKPISYTTTFSHQVIC*N *M*EKSARGKL*GTFNVT*KRNGK*EHSKYSEH N*P**H*RKCF*RSQLKQY**SRFQY**SGLQY **NRFQ**KHSSRTR*KQRAKIECYA*IRGFAT* GL*TKSSWK*L*AS*NKKARI*RSSSDC*YRFL SISDFR*LRTAYGK*SCISGLF*DT**PVR*W* NKGRY*FC*K*H*GKFCCF*QKRPERRA*QES*P FHPYTFGSGLPKRGQEIRVLRRELI**G*RASL LPTLVIW*SKQYTFSVY*A*HRCYRVSV*EHRG EFIIIEE*LK*LQ*PGNIGKGISGTSP**GNKMF C*LVFFTVQ*IGRLDCKYKHPGSFLDWFFQTNE ASV*KPGSWSE*QGIGFR**RKRNGLGRK*SRR AKHGFKLR*SSIWV*E*NKRL*RLLRAILSE*HF NHSAEGYHAT*PDKAPAGNG*TRSCVRTAWEPA F*QLPFHHK*LFCP*GPAKSRTKHIRKSSINFT EK**IPYKPESRRPFC*QV*GVCR*FYQ*K*RTR SGKVIPF*MPIIR**VVHAQLLWESSE*KLPIS RGAH*GC*CGGATAGRVWATRFDGNILLAKARS RGNPLPGIWNQPLL**P*I*SF*RQSPRVSSCWQ HTIFNLCIESSPIESCRICPESSCCSYY*YCWV *CNGRKCEQGEARIDSFNRKGQQKNVHGGVWPD PRRIYARVQVCQKTPHHFN*SNY*RDYSCCYENR C*VCV*TDTEIFSRNCGRKMGS*LFLGDPVY*R KKNAE*A*F*SQRRCGQWKKPPRSKASKRIPGQ KDLQGARNLLLWALHQHAHRSTGMDGTAVWCFCG EGAFIIHPWHRCPPNCGCAARCLDRGQWLPCNW ADV*GTCGDPRVGVGQCSTLPVPGAGHLPDTPD PPQPLLTAASHRYRATGPQE*AYKVAFPGPGSSS HSSVLLLSWLLNILCTSA*KGLLAMQGSLKDFL LEVSLGNLP*AQNYGNFSPEKILKPFKRHQLSK MLIHYLSALFFLFRLLLA*GWKHRVAWPQENSWF P*VYFSKTLCSQRQRVRPFNGRRVLGIDYVT*S QNSPWAVLKCWSGTLGRKF*GRY*K*KGNLKPG HGGSRL*SQHFGRPRWADHWRSGVRNQPGQHGET PSLLKIQKLAGHGGGHL*SQLLRWLRQENHFSP GGGGCSEPRSYHGTPAWVTVRLWLKKKKKKRKM KLEEISKSLRYIC*ISKECVLKQQKIFKNRFPKT VF*FLISNK*NVYCCSSGI*SIPLKI*DLWHEY FISIK*QIPPGRKLLLSLR*FFSFAPCC*NHTA S*IILLAEGRKSVFHKPIIQDCL*LLEGLGLP*P PQCARAVKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL CSGSLEQKEMDLSALRVEEVQNVINAMQKILECP ICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGP SQCPLCKNDITKRSLQESTRFSQLVEELLKIIC AFQLDTGLEYANSYNFAKKENNSPEHLKDEVSII QSMGYRNRAKRLLQSEPENPSLQETSLSVQLSN LGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTV NKATYCSVGDQELLQITPQGTRDEISLDSAKKAA CEFSETDVTNTEHHQPSNNDLNTTEKRAAERHP EKYQGSSVSNLHVEPCGTNTHASSLQHENSSLL LTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSK ETCNDRRTPSTEKKVDLNADPLCERKEWNKQKL PCSENPRDTEDVPWITLNSSIQKVNEWFSRSDE LLGSDDSHDGESESNAKVADVLDVLNEVDEYSGS SEKIDLLASDPHEALICKSERVHSKSVESNIED KIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQ IIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAV QKTPEMINQGTNQTEQNGQVMNITNSGHENKTK GDSIQNEKNPNPIESLEKESAFKTKAEPISSSI SNMELELNIHNSKAPKKNRLRRKSSTRHIHALEL VVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQM PVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKR HDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPS LPREEKEEKLETVKVSNNAEDPKDLMLSGERVL QTERSVESSSISLVPGTDYGTQESISLLEVSTL GKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRND TEGFKYPLGHEVNHSRETSIEMEESELDAQYLQ NTFKVSKRQSFAPFSNPGNAEEECATFSAHSGS LKKQSPKVTFECEQKEENQGKNESNIKPVQTVNI TAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQF RGNETGLITPNKHGLLQNPYRIPPLFPIKSFVK TKCKKNLLEENFEEHSMSPEREMGNENIPSTVST ISRNNIRENVFKEASSSNINEVGSSTNEVGSSI NEIGSSDENIQAELGRNRGPKLNAMLRLGVLQP EVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFS PYLISDNLEQPMGSSHASQVCSETPDDLLDDGE IKEDTSFAENDIKESSAVFSKSVQKGELSRSPS PFTHTHLAQGYRRGAKKLESSEENLSSEDEELPC FQHLLFGKVNNIPSQSTRHSTVATECLSKNTEE NLLSLKNSLNDCSNQVILAKASQEHHLSEETKC SASLFSSQCSELEDLTANTNTQDPFLIGSSKQMR HQSESQGVGLSDKELVSDDEERGTGLEENNQEE QSMDSNLGEAASGCESETSVSEDCSGLSSQSDI LTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQP SNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQ KSSEYPISQNPEGLSADKFEVSADSSTSKNKEP GVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQ EELIKVVDVEEQQLEESGPHDLTETSYLPRQDL EGTPYLESGISLFSDDPESDPSEDRAPESARVG NIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGY NAMEESVSREKPELTASTERVNKRMSMVVSGLT PEEFMLVYKFARKHHITLTNLITEETTHVVMKT DAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKE RKMLNEHDFEVRGDVVNGRNHQGPKRARESQDR KIFRGLEICCYGPFTNMPTDQLEWMVQLCGASV VKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIG QMCEAPVVTREWVLDSVALYQCQELDTYLIPQI PHSHY*LQPATGTEPQDPKNELTKWPFQALGAP LTLQSFYCPGY*IFYVHQPEKDFWLCKGPLKIFC LKSPLEICHEHKIMVIFHLRRF*NHLNATN*AR C*FIIYQPYSFYSGCCWLRAGSTEWLGLKRIAG FPKFTSLKPCVHKGRESDPSMEGECLGSIM*LKV RIVLGQFSNVGVEHWGGNSEAGIRNEKET*NLG MVAHACNPSTLGGQGGQITGGQEFETSLANMVK PHLY*KYRN*PVMVVDTCNPSYSGG*GRRITSAR EVEVAVSQDHTTALQPG*Q*DCGSKKKKKKGK* N*KRFLKV*DIFARFLKNVF*NSRRFSRTGFQR QSSNSSLVISKMFIVVALVYNPFLLKYKTSGMNI SYL*NDRSHQEGSCCFL*GDFFPLLPVAETIQL HK*FCLLKEEKVFFINPLSRTVYSCWKD*VFPS PPSVQGQ*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA TARRELAETSWTGDRLWGFSDNWAPALRRPSPS ALVHWNRKKWIYLLFALKKYKMSLMLCRKS*SV PSVWS*SRNLSPQSVTTYFANFAC*NFSTRRKGL HSVLYVRMI*PKGAYKKVRDLVNLLKSY*KSFV LFSLTQVWSMQTAIILQKRKITLLNI*KMKFLS SKVWATETVPKDFYRVNPKILPCRKPVSVSNSLT LEL*EL*GQSSGYNLKRRLSTLNWDLILLKIPL IRQLIAVWEIKNCYKSPLKEPGMKSVWILQKRL LVNFLRRM*QILNIINPVIMI*TPLRSVQLRGIQ KSIRVVLFQTCMWSHVAQILMPAHYSMRTAVYY SLKTE*M*KRLNSVIKANSLA*QGANITDGLEV RKHVMIGGLPAQKKR*I*MLIPCVREKNGISRNC HAQRILEILKMFLG*H*IAAFRKLMSGFPEVMN C*VLMTHMMGSLNQMPK*LMYWTF*MR*MNILV LQRK*TYWPVILMRL*YVKVKEFTPNQ*RVILKT KYLGKPIGRRQASPT*AM*LKI*L*EHLLLSHR *YKSVPSQIN*SVKGDLHQAFILRILSRKQIWQ FKRLLK**IRELTKRSRMVK**ILLIVVMRIKQK VILFRMRKILTQ*NHSKKNLLSKRKLNL*AAV* AIWNSN*ISTIQKHLKRIG*GGSLLPGIFMRLN **SVEI*AHLIVLNCKLIVVLAVKR*RKKSTTKC QSGTAETYNSWKVKNLQLEPRRVTSQMNRQVKD MTAILSQS*S*QMHLVLLLSVQIPVNLKNLSIL AFQEKKKKRN*KQLKCLIMLKTPKISC*VEKGFC KLKDL*RVAVFHWYLVLIMALRKVSRYWKLAL* GRQKQNQINV*VSVQHLKTPRD*FMVVPKIIEM TQKALSIHWDMKLTTVGKQA*KWKKVNLMLSICR IHSRFQSASHLLRFQIQEMQKRNVQHSLPTLGP *RNKVQKSLLNVNKRKKIKERMSLISSLYRQLI SLQAFLWLVRKISQLIMPNVVSKEALGFVYHLSS EATKLDSLLQINMDFYKTHIVYHHFFPSSHLLK LNVRKIC*RKTLRNIQCHLKEKWEMRTFQVQ*A QLAVITLEKMFLKKPAQAILMK*VPVLMKWAPVL MK*VPVMKTFKQN*VETEGQN*MLCLD*GFCNL RSINKVFLEVIVSILK*KSKNMKK*FRLLIQIS LHI*FQIT*NSLWEVVMHLRFVLRHLMTC*MMVK *RKILVLLKMTLRKVLLFLAKASRKESLAGVLA LSPIHIWLRVTEEGPRN*SPQKRTYLVRMKSFP ASNTCYLVK*TIYLLSLLGIAPLLPSVCLRTQRR IYYH*RIA*MTAVTR*YWQRHLRNITLVRKQNV LLACFLHSAVNWKT*LQIQTPRILS*LVLPNK* GISLKARELV*VTRNWFQMMKKEERAWKKIIKKS KAWIQT*VKQHLGVRVKQASLKTAQGYPLRVTF *PLSRGIPCNIT**SSSRKWLN*KLC*NSMGAS LLTATLPS*VTLLPLRTCEIQNKAHQKKQY*LHR KVVNTL*ARIQKAFLLTSLRCLQIVLPVKIKNQ EWKGHPLLNAHH*MIGGTCTVALGVFRIETTHL KRSSLRLLMWRSNSWKSLGHTI*RKHLTCQGKI* REPLTWNLESASSLMTLNLILLKTEPQSQLVLA TYHLQPLH*KFPN*KLQNLPRVQLLLILLILLG IMQWKKV*AGRSQN*QLQQKGSTKECPWWCLA*P QKNLCSCTSLPENTTSL*LI*LLKRLLMLL*KQ MLSLCVNGH*NIF*ELREENG*LAISG*PSLLK KEKC*MSMILKSEEMWSMEETTKVQSEQENPRTE RSSGG*KSVAMGPSPTCPQINWNGWYSCVVLLW *RSFHHSPLAQVSTQLWLCSQMPGQRTMASMQL GRCVRHLW*PESGCWTV*HSTSARSWTPT*YPRS PTATTDCSQPQVQSHRTPRMSLQSGLSRPWELL SLFSPSTVLATKYFMYISLKRTSGYARVP*RFS A*SLPWKSAMSTKLW*FFT*EDFKTI*TPPIEQD ADSLFISPILSIQAVVGLGLEAQSGLASRE*LV SLSLLL*NPVFTKAESQTLQWKESAWDRLCDLK SE*SLGSSQMLEWNIGEEILRQVLEMKRKLETWA WWLTPVIPALWEAKVGRSLEVRSSKPAWPTW*N PISTKNTEISRSWWWTPVIPATQVAKAGESLQP GRWRLQ*AKIIPRHSSLGDSETVAQKKKKKKENE TRRDF*KSEIYLLDF*RMCSKTAEDFQEPVSKD SLLIPH***VKCLLL*LWYIIHSS*NIRPLA*I FHIYKMTDPTRKEAVAFFEVIFFLCSLLLKPYSF INNFAC*RKKKCFS*THYPGLFIAVGRTRSSLA PPVCKGSEDLIVQNTFCKCCAVNTANKLGSKHF

Sequence BRCA1.aAug05

GAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCTGCTCTGGGTAAAGGTAGTAGAG TCCCGGGAAAGGGACAGGGGGCCCAAGTGATGCTCTGGGGTACTGGCGTGGGAGAGTGGATTTCCGAAGCTGACAGATGGGTATTCTTTGACGGGGGGTA GGGGCGGAACCTGAGAGGCGTAAGGCGTTGTGAACCCTGGGGAGGGGGGCAGTTTGTAGGTCGCGAGGGAAGCGCTGAGGATCAGGAAGGGGGCACTGAG TGTCCGTGGGGGAATCCTCGTGATAGGAACTGGAATATGCCTTGAGGGGGACACTATGTCTTTAAAAACGTCGGCTGGTCATGAGGTCAGGAGTTCCAGA CCAGCCTGACCAACGTGGTGAAACTCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGCCGCTCCAGCTACTCAGGAGGCTGAGGCAGGAGAA TCGCTAGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCATTGCACTCCAGCCTGGGCGACAGAGCGAGACTGTCTCAAAACAAAACAAAAC AAAACAAAACAAAAAACACCGGCTGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCA GAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTC AACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGC TATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAA AGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTC AGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATT CTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGA TTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGT GCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCTCATTACAGCATG AGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAA CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAA TGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAGTTAATGAGTGGT TTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGT AGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCACTCCAAATCAGTA GAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCAT TTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAA GAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGT CATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGAAC CTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTCTTCTACCAGGCA TATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAA AAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATG AACAGACAAGTAAAAGACATGACAGCGATACTTTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAA AGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTA AGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTAC TGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGTTC CAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAAGAAAGTGAACTT GATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCT CTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTCTAATATCAAGCC TGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTT TGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCACCACTTTTTCCCA TCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACAT TCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTAAT GAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATGCTATGCTTAGAT TAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGAC TGTTAATACAGATTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGATGAC CTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGC TTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAACTTATCTAGTGA GGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTG TCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCTTA GTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAGGATCCTTTCTTGATTGG TTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAA GAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTAT CCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGA ACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAA GCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCA GTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAA TAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTAC TTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAG AGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATAC TACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATG GTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTC ATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGT GACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCA AGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGC TGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAA TGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACC TACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCC AGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGG TCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAAC GCCACCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCC TCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATC GATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAG GAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCC TGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAG GAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAA AAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAA GAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAA GACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTT GCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGG ACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAAC TTGGTAGCAAACACTTCCA

BRCA1-F2 (forward primer)
BRCA1-R2 (reverse primer)
Multiple exon skipping

Frame 0

ETSWTGDRLWGFSDNWAPALRRPSPSALGKGSR VPGKGQGAQVMLWGTGVGEWISEADRWVFFDGG* GRNLRGVRRCEPWGGGQFVGREGSAEDQEGGTE CPWGNPRDRNWNMP*GGHYVFKNVGWS*GQEFQ TSLTNVVKLRLY*KYKN*PGVVPLQLLRRLRQEN R*NPGGGGCSEPRSRHCTPAWATERDCLKTKQN KTKQKTPAVHWNRKKWIYLLFALKKYKMSLMLC RKS*SVPSVWS*SRNLSPQSVTTYFANFAC*NFS TRRKGLHSVLYVRMI*PKGAYKKVRDLVNLLKS Y*KSFVLFSLTQVWSMQTAIILQKRKITLLNI* KMKFLSSKVWATETVPKDFYRVNPKILPCRKPVS VSNSLTLEL*EL*GQSSGYNLKRRLSTLNWDLI LLKIPLIRQLIAVWEIKNCYKSPLKEPGMKSVW ILQKRLLVNFLRRM*QILNIINPVIMI*TPLRSV QLRGIQKSIRVVLFQTCMWSHVAQILMPAHYSM RTAVYYSLKTE*M*KRLNSVIKANSLA*QGANI TDGLEVRKHVMIGGLPAQKKR*I*MLIPCVREKN GISRNCHAQRILEILKMFLG*H*IAAFRKLMSG FPEVMNC*VLMTHMMGSLNQMPK*LMYWTF*MR *MNILVLQRK*TYWPVILMRL*YVKVKEFTPNQ* RVILKTKYLGKPIGRRQASPT*AM*LKI*L*EH LLLSHR*YKSVPSQIN*SVKGDLHQAFILRILS RKQIWQFKRLLK**IRELTKRSRMVK**ILLIVV MRIKQKVILFRMRKILTQ*NHSKKNLLSKRKLN L*AAV*AIWNSN*ISTIQKHLKRIG*GGSLLPG IFMRLN**SVEI*AHLIVLNCKLIVVLAVKR*RK KSTTKCQSGTAETYNSWKVKNLQLEPRRVTSQM NRQVKDMTAILSQS*S*QMHLVLLLSVQIPVNL KNLSILAFQEKKKKRN*KQLKCLIMLKTPKISC* VEKGFCKLKDL*RVAVFHWYLVLIMALRKVSRY WKLAL*GRQKQNQINV*VSVQHLKTPRD*FMVV PKIIEMTQKALSIHWDMKLTTVGKQA*KWKKVNL MLSICRIHSRFQSASHLLRFQIQEMQKRNVQHS LPTLGP*RNKVQKSLLNVNKRKKIKERMSLISS LYRQLISLQAFLWLVRKISQLIMPNVVSKEALGF VYHLSSEATKLDSLLQINMDFYKTHIVYHHFFP SSHLLKLNVRKIC*RKTLRNIQCHLKEKWEMRT FQVQ*AQLAVITLEKMFLKKPAQAILMK*VPVLM KWAPVLMK*VPVMKTFKQN*VETEGQN*MLCLD *GFCNLRSINKVFLEVIVSILK*KSKNMKK*FR LLIQISLHI*FQIT*NSLWEVVMHLRFVLRHLMT C*MMVK*RKILVLLKMTLRKVLLFLAKASRKES LAGVLALSPIHIWLRVTEEGPRN*SPQKRTYLV RMKSFPASNTCYLVK*TIYLLSLLGIAPLLPSVC LRTQRRIYYH*RIA*MTAVTR*YWQRHLRNITL VRKQNVLLACFLHSAVNWKT*LQIQTPRILS*L VLPNK*GISLKARELV*VTRNWFQMMKKEERAWK KIIKKSKAWIQT*VKQHLGVRVKQASLKTAQGY PLRVTF*PLSRGIPCNIT**SSSRKWLN*KLC* NSMGASLLTATLPS*VTLLPLRTCEIQNKAHQKK QY*LHRKVVNTL*ARIQKAFLLTSLRCLQIVLP VKIKNQEWKGHPLLNAHH*MIGGTCTVALGVFR IETTHLKRSSLRLLMWRSNSWKSLGHTI*RKHLT CQGKI*REPLTWNLESASSLMTLNLILLKTEPQ SQLVLATYHLQPLH*KFPN*KLQNLPRVQLLLI LLILLGIMQWKKV*AGRSQN*QLQQKGSTKECPW WCLA*PQKNLCSCTSLPENTTSL*LI*LLKRLL MLL*KQMLSLCVNGH*NIF*ELREENG*LAISG *PSLLKKEKC*MSMILKSEEMWSMEETTKVQSEQ ENPRTERSSGG*KSVAMGPSPTCPQINWNGWYS CVVLLW*RSFHHSPLAQVSTQLWLCSQMPGQRT MASMQLGRCVRHLW*PESGCWTV*HSTSARSWTP T*YPRSPTATTDCSQPQVQSHRTPRMSLQSGLS RPWELLSLFSPSTVLATKYFMYISLKRTSGYAR VP*RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*T PPIEQDADSLFISPILSIQAVVGLGLEAQSGLA SRE*LVSLSLLL*NPVFTKAESQTLQWKESAWD RLCDLKSE*SLGSSQMLEWNIGEEILRQVLEMKR KLETWAWWLTPVIPALWEAKVGRSLEVRSSKPA WPTW*NPISTKNTEISRSWWWTPVIPATQVAKA GESLQPGRWRLQ*AKIIPRHSSLGDSETVAQKKK KKKENETRRDF*KSEIYLLDF*RMCSKTAEDFQ EPVSKDSLLIPH***VKCLLL*LWYIIHSS*NI RPLA*IFHIYKMTDPTRKEAVAFFEVIFFLCSLL LKPYSFINNFAC*RKKKCFS*THYPGLFIAVGR TRSSLAPPVCKGSEDLIVQNTFCKCCAVNTANK LGSKHF

Frame 1

RLPGRGTGCGVSQITGPLRSGGLHPLLWVKVVE SRERDRGPK*CSGVLAWESGFPKLTDGYSLTGG RGGT*EA*GVVNPGEGGSL*VAREALRIRKGALS VRGGILVIGTGICLEGDTMSLKTSAGHEVRSSR PA*PTW*NSVSTKNTKISRAWCRSSYSGG*GRR IARTREAEVAVSRDRAIALQPGRQSETVSKQNKT KQNKKHRLFIGTERNGFICSSR*RSTKCH*CYA ENLRVSHLSGVDQGTCLHKV*PHILQILHAETS QPEERAFTVSFM*E*YNQKEPTRKYEI*STC*RA IENHLCFSA*HRFGVCKQL*FCKKGK*LS*TSK R*SFYHPKYGLQKPCQKTSTE*TRKSFLAGNQS QCPTL*PWNCENSEDKAADTTSKDVCLH*IGI*F F*RYR**GNLLQCGRSRIVTNHPSRNQG*NQFG FCKKGCL*IF*DGCNKY*TSSTQ***FEHH*EA CS*EASRKVSG*FCFKLACGAMWHKYSCQLITA* EQQFITH*RQNECRKG*IL**KQTAWLSKEPT* QMGWK*GNM***ADSQHRKKGRSEC*SPV*EKR ME*AETAMLRES*RY*RCSLDNTK*QHSES**VV FQK**TVRF**LT*WGV*IKCQSS*CIGRSK*G R*IFWFFRENRLTGQ*SS*GFNM*K*KSSLQIS RE*Y*RQNIWENLSEEGKPPQLKPCN*KSNYRSI CY*ATDNTRASPHK*IKA*KETYIRPSS*GFYQ ESRFGSSKDS*NDKSGN*PNGAEWSSDEYY**W S*E*NKR*FYSE*EKS*PNRITRKRICFQNES*T YKQQYKQYGTRIKYPQFKST*KE*AEEEVFYQA YSCA*TSSQ*KSKPT*LY*IAN**LF*Q*RDKE KKVQPNASQAQQKPTTHGR*RTCNWSQEE*QAK* TDK*KT*QRYFPRAEVNKCTWFFY*VFKYQ*T* RICQS*PSKRRKRRETRNS*SV**C*RPQRSHV KWRKGFAN*KICRE*QYFIGTWY*LWHSGKYLVT GS*HSREGKNRTK*MCESVCSI*KPQGTNSWLF QR**K*HRRL*VSIGT*S*PQSGNKHRNGRK*T *CSVFAEYIQGFKAPVICSVFKSRKCRRGMCNIL CPLWVLKETKSKSHF*M*TKGRKSRKE*V*YQA CTDS*YHCRLSCGWSER*AS**CQM*YQRRL*V LSIISVQRQRNWTHYSK*TWTFTKPISYTTTFSH QVIC*N*M*EKSARGKL*GTFNVT*KRNGK*EH SKYSEHN*P**H*RKCF*RSQLKQY**SRFQY* *SGLQY**NRFQ**KHSSRTR*KQRAKIECYA*I RGFAT*GL*TKSSWK*L*AS*NKKARI*RSSSD C*YRFLSISDFR*LRTAYGK*SCISGLF*DT** PVR*W*NKGRY*FC*K*H*GKFCCF*QKRPERRA *QES*PFHPYTFGSGLPKRGQEIRVLRRELI** G*RASLLPTLVIW*SKQYTFSVY*A*HRCYRVS V*EHRGEFIIIEE*LK*LQ*PGNIGKGISGTSP* *GNKMFC*LVFFTVQ*IGRLDCKYKHPGSFLDW FFQTNEASV*KPGSWSE*QGIGFR**RKRNGLG RK*SRRAKHGFKLR*SSIWV*E*NKRL*RLLRAI LSE*HFNHSAEGYHAT*PDKAPAGNG*TRSCVR TAWEPAF*QLPFHHK*LFCP*GPAKSRTKHIRK SSINFTEK**IPYKPESRRPFC*QV*GVCR*FYQ *K*RTRSGKVIPF*MPIIR**VVHAQLLWESSE *KLPISRGAH*GC*CGGATAGRVWATRFDGNIL LAKARSRGNPLPGIWNQPLL**P*I*SF*RQSPR VSSCWQHTIFNLCIESSPIESCRICPESSCCSY Y*YCWV*CNGRKCEQGEARIDSFNRKGQQKNVH GGVWPDPRRIYARVQVCQKTPHHFN*SNY*RDYS CCYENRC*VCV*TDTEIFSRNCGRKMGS*LFLG DPVY*RKKNAE*A*F*SQRRCGQWKKPPRSKAS KRIPGQKDLQGARNLLLWALHQHAHRSTGMDGTA VWCFCGEGAFIIHPWHRCPPNCGCAARCLDRGQ WLPCNWADV*GTCGDPRVGVGQCSTLPVPGAGH LPDTPDPPQPLLTAASHRYRATGPQE*AYKVAFP GPGSSSHSSVLLLSWLLNILCTSA*KGLLAMQG SLKDFLLEVSLGNLP*AQNYGNFSPEKILKPFK RHQLSKMLIHYLSALFFLFRLLLA*GWKHRVAWP QENSWFP*VYFSKTLCSQRQRVRPFNGRRVLGI DYVT*SQNSPWAVLKCWSGTLGRKF*GRY*K*K GNLKPGHGGSRL*SQHFGRPRWADHWRSGVRNQP GQHGETPSLLKIQKLAGHGGGHL*SQLLRWLRQ ENHFSPGGGGCSEPRSYHGTPAWVTVRLWLKKK KKKRKMKLEEISKSLRYIC*ISKECVLKQQKIFK NRFPKTVF*FLISNK*NVYCCSSGI*SIPLKI* DLWHEYFISIK*QIPPGRKLLLSLR*FFSFAPC C*NHTAS*IILLAEGRKSVFHKPIIQDCL*LLEG LGLP*PPQCARAVKT*LYKIRFVNVVLLTLQIN LVANTS

Frame 2

DFLDGGQAVGFLR*LGPCAQEAFTLCSG*R**S PGKGTGGPSDALGYWRGRVDFRS*QMGIL*RGV GAEPERRKAL*TLGRGAVCRSRGKR*GSGRGH* VSVGESS**ELEYALRGTLCL*KRRLVMRSGVPD QPDQRGETPSLLKIQKLAGRGAAPATQEAEAGE SLEPGRRRLQ*AEIAPLHSSLGDRARLSQNKTK QNKTKNTGCSLEQKEMDLSALRVEEVQNVINAMQ KILECPICLELIKEPVSTKCDHIFCKFCMLKLL NQKKGPSQCPLCKNDITKRSLQESTRFSQLVEE LLKIICAFQLDTGLEYANSYNFAKKENNSPEHLK DEVSIIQSMGYRNRAKRLLQSEPENPSLQETSL SVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSD SSEDTVNKATYCSVGDQELLQITPQGTRDEISLD SAKKAACEFSETDVTNTEHHQPSNNDLNTTEKR AAERHPEKYQGSSVSNLHVEPCGTNTHASSLQH ENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHN RWAGSKETCNDRRTPSTEKKVDLNADPLCERKE WNKQKLPCSENPRDTEDVPWITLNSSIQKVNEW FSRSDELLGSDDSHDGESESNAKVADVLDVLNEV DEYSGSSEKIDLLASDPHEALICKSERVHSKSV ESNIEDKIFGKTYRKKASLPNLSHVTENLIIGA FVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIK KADLAVQKTPEMINQGTNQTEQNGQVMNITNSG HENKTKGDSIQNEKNPNPIESLEKESAFKTKAE PISSSISNMELELNIHNSKAPKKNRLRRKSSTRH IHALELVVSRNLSPPNCTELQIDSCSSSEEIKK KKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPN EQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELK EFVNPSLPREEKEEKLETVKVSNNAEDPKDLML SGERVLQTERSVESSSISLVPGTDYGTQESISL LEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCS KDNRNDTEGFKYPLGHEVNHSRETSIEMEESEL DAQYLQNTFKVSKRQSFAPFSNPGNAEEECATF SAHSGSLKKQSPKVTFECEQKEENQGKNESNIKP VQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRF CLSSQFRGNETGLITPNKHGLLQNPYRIPPLFP IKSFVKTKCKKNLLEENFEEHSMSPEREMGNENI PSTVSTISRNNIRENVFKEASSSNINEVGSSTN EVGSSINEIGSSDENIQAELGRNRGPKLNAMLR LGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQT VNTDFSPYLISDNLEQPMGSSHASQVCSETPDD LLDDGEIKEDTSFAENDIKESSAVFSKSVQKGE LSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSE DEELPCFQHLLFGKVNNIPSQSTRHSTVATECL SKNTEENLLSLKNSLNDCSNQVILAKASQEHHL SEETKCSASLFSSQCSELEDLTANTNTQDPFLIG SSKQMRHQSESQGVGLSDKELVSDDEERGTGLE ENNQEEQSMDSNLGEAASGCESETSVSEDCSGL SSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLE QHGSQPSNSYPSIISDSSALEDLRNPEQSTSEK AVLTSQKSSEYPISQNPEGLSADKFEVSADSST SKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQN RNYPSQEELIKVVDVEEQQLEESGPHDLTETSY LPRQDLEGTPYLESGISLFSDDPESDPSEDRAP ESARVGNIPSSTSALKVPQLKVAESAQSPAAAHT TDTAGYNAMEESVSREKPELTASTERVNKRMSM VVSGLTPEEFMLVYKFARKHHITLTNLITEETT HVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWV TQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRA RESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQ LCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDN GFHAIGQMCEAPVVTREWVLDSVALYQCQELDT YLIPQIPHSHY*LQPATGTEPQDPKNELTKWPF QALGAPLTLQSFYCPGY*IFYVHQPEKDFWLCKG PLKIFCLKSPLEICHEHKIMVIFHLRRF*NHLN ATN*ARC*FIIYQPYSFYSGCCWLRAGSTEWLG LKRIAGFPKFTSLKPCVHKGRESDPSMEGECLGS IM*LKVRIVLGQFSNVGVEHWGGNSEAGIRNEK ET*NLGMVAHACNPSTLGGQGGQITGGQEFETS LANMVKPHLY*KYRN*PVMVVDTCNPSYSGG*GR RITSAREVEVAVSQDHTTALQPG*Q*DCGSKKK KKKGK*N*KRFLKV*DIFARFLKNVF*NSRRFS RTGFQRQSSNSSLVISKMFIVVALVYNPFLLKYK TSGMNISYL*NDRSHQEGSCCFL*GDFFPLLPV AETIQLHK*FCLLKEEKVFFINPLSRTVYSCWK D*VFPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*T W*QTLP

Sequence BRCA1.nAug05

Frame 0

Frame 1

Frame 2

Sequence BRCA1.dAug05

Frame 0

Frame 1

Frame 2

Sequence BRCA1.jAug05

Frame 0

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Sequence BRCA1.iAug05

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Sequence BRCA1.hAug05

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Sequence BRCA1.fAug05

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Sequence BRCA1.eAug05

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Sequence BRCA1.cAug05

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Sequence BRCA1.bAug05

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Sequence BRCA1.aAug05

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Sequence BRCA1.qAug05

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Sequence BRCA1.oAug05

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Sequence BRCA1.lAug05

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