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Sequences

Sequence BRCA1.mAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA
GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA
GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA
TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC
TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC
TCT
AACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA
CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA
GGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGAT
ACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCA
TCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTAT
AAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGTCATCCCCT
TCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTG
TTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGA
ATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCT
GCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTG
TGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTTATGCTCGT
GTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAA
CGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCTGAATGAGC
ATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGA
AATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTC
ACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATGTGTGAGGCACCTG
TGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACT
GCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTAC
TGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAA
TCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCATTATTTATCA
GCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTA
AAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGT
TCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTGGCTCACGCCTGTAA
TCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATAC
AGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGA
GCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAA
AGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAATTCCTCATTA
GTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATATCTATAAAATGACA
GATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCT
GAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAG
TGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR
VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE
RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH
LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF
YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT
L
*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY
R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK
G*SSIWV*E*NKRL*RLLRAILSE*HFNHSAEG
YHAT*PDKAPAGNG*TRSCVRTAWEPAF*QLPFH
HK*LFCP*GPAKSRTKHIRKSSINFTEK**IPY
KPESRRPFC*QV*GVCR*FYQ*K*RTRSGKVIP
F*MPIIR**VVHAQLLWESSE*KLPISRGAH*GC
*CGGATAGRVWATRFDGNILLAKARSRGNPLPG
IWNQPLL**P*I*SF*RQSPRVSSCWQHTIFNL
CIESSPIESCRICPESSCCSYY*YCWV*CNGRKC
EQGEARIDSFNRKGQQKNVHGGVWPDPRRIYAR
VQVCQKTPHHFN*SNY*RDYSCCYENRC*VCV*
TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNAE*A
*F*SQRRCGQWKKPPRSKASKRIPGQKDLQGAR
NLLLWALHQHAHRSTGMDGTAVWCFCGEGAFII
HPWHRCPPNCGCAARCLDRGQWLPCNWADV*GTC
GDPRVGVGQCSTLPVPGAGHLPDTPDPPQPLLT
AASHRYRATGPQE*AYKVAFPGPGSSSHSSVLL
LSWLLNILCTSA*KGLLAMQGSLKDFLLEVSLGN
LP*AQNYGNFSPEKILKPFKRHQLSKMLIHYLS
ALFFLFRLLLA*GWKHRVAWPQENSWFP*VYFS
KTLCSQRQRVRPFNGRRVLGIDYVT*SQNSPWAV
LKCWSGTLGRKF*GRY*K*KGNLKPGHGGSRL*
SQHFGRPRWADHWRSGVRNQPGQHGETPSLLKI
QKLAGHGGGHL*SQLLRWLRQENHFSPGGGGCSE
PRSYHGTPAWVTVRLWLKKKKKKRKMKLEEISK
SLRYIC*ISKECVLKQQKIFKNRFPKTVF*FLI
SNK*NVYCCSSGI*SIPLKI*DLWHEYFISIK*Q
IPPGRKLLLSLR*FFSFAPCC*NHTAS*IILLA
EGRKSVFHKPIIQDCL*LLEGLGLP*PPQCARA
VKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE
CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK
GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI
ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS
IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL
S
NLGTVRTLRTKQRIQPQKTSVYIELGSDSSED
TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK
GEAASGCESETSVSEDCSGLSSQSDILTTQQRD
TMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPS
IISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPI
SQNPEGLSADKFEVSADSSTSKNKEPGVERSSP
SKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKV
VDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLE
SGISLFSDDPESDPSEDRAPESARVGNIPSSTS
ALKVPQLKVAESAQSPAAAHTTDTAGYNAMEES
VSREKPELTASTERVNKRMSMVVSGLTPEEFMLV
YKFARKHHITLTNLITEETTHVVMKTDAEFVCE
RTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNE
HDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLE
ICCYGPFTNMPTDQLEWMVQLCGASVVKELSSF
TLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAP
VVTREWVLDSVALYQCQELDTYLIPQIPHSHY*L
QPATGTEPQDPKNELTKWPFQALGAPLTLQSFY
CPGY*IFYVHQPEKDFWLCKGPLKIFCLKSPLE
ICHEHKIMVIFHLRRF*NHLNATN*ARC*FIIYQ
PYSFYSGCCWLRAGSTEWLGLKRIAGFPKFTSL
KPCVHKGRESDPSMEGECLGSIM*LKVRIVLGQ
FSNVGVEHWGGNSEAGIRNEKET*NLGMVAHACN
PSTLGGQGGQITGGQEFETSLANMVKPHLY*KY
RN*PVMVVDTCNPSYSGG*GRRITSAREVEVAV
SQDHTTALQPG*Q*DCGSKKKKKKGK*N*KRFLK
V*DIFARFLKNVF*NSRRFSRTGFQRQSSNSSL
VISKMFIVVALVYNPFLLKYKTSGMNISYL*ND
RSHQEGSCCFL*GDFFPLLPVAETIQLHK*FCLL
KEEKVFFINPLSRTVYSCWKD*VFPSPPSVQGQ
*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS*
SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK
GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS
FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF
LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS
L
TLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI
PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK
RVKQHLGVRVKQASLKTAQGYPLRVTF*PLSRGI
PCNIT**SSSRKWLN*KLC*NSMGASLLTATLP
S*VTLLPLRTCEIQNKAHQKKQY*LHRKVVNTL
*ARIQKAFLLTSLRCLQIVLPVKIKNQEWKGHPL
LNAHH*MIGGTCTVALGVFRIETTHLKRSSLRL
LMWRSNSWKSLGHTI*RKHLTCQGKI*REPLTW
NLESASSLMTLNLILLKTEPQSQLVLATYHLQPL
H*KFPN*KLQNLPRVQLLLILLILLGIMQWKKV
*AGRSQN*QLQQKGSTKECPWWCLA*PQKNLCS
CTSLPENTTSL*LI*LLKRLLMLL*KQMLSLCVN
GH*NIF*ELREENG*LAISG*PSLLKKEKC*MS
MILKSEEMWSMEETTKVQSEQENPRTERSSGG*
KSVAMGPSPTCPQINWNGWYSCVVLLW*RSFHHS
PLAQVSTQLWLCSQMPGQRTMASMQLGRCVRHL
W*PESGCWTV*HSTSARSWTPT*YPRSPTATTD
CSQPQVQSHRTPRMSLQSGLSRPWELLSLFSPST
VLATKYFMYISLKRTSGYARVP*RFSA*SLPWK
SAMSTKLW*FFT*EDFKTI*TPPIEQDADSLFI
SPILSIQAVVGLGLEAQSGLASRE*LVSLSLLL*
NPVFTKAESQTLQWKESAWDRLCDLKSE*SLGS
SQMLEWNIGEEILRQVLEMKRKLETWAWWLTPV
IPALWEAKVGRSLEVRSSKPAWPTW*NPISTKNT
EISRSWWWTPVIPATQVAKAGESLQPGRWRLQ*
AKIIPRHSSLGDSETVAQKKKKKKENETRRDF*
KSEIYLLDF*RMCSKTAEDFQEPVSKDSLLIPH*
**VKCLLL*LWYIIHSS*NIRPLA*IFHIYKMT
DPTRKEAVAFFEVIFFLCSLLLKPYSFINNFAC
*RKKKCFS*THYPGLFIAVGRTRSSLAPPVCKGS
EDLIVQNTFCKCCAVNTANKLGSKHF

Sequence BRCA1.qAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCC
CATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCT
TCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTG
CTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTCTATCAT
CCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTT
GGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATACCGTTAATA
AGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGCTTG
TGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAA
AAGTATCAGG
GTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGC
AGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTA
CCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAA
TACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGT
CATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCAT
TAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCT
TACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTT
CAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGA
AGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTT
ATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTG
TGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWFIGTERNGFICSSR*RSTKCH*CYAENLRVS
HLSGVDQGTCLHKV*PHILQILHAETSQPEERA
FTVSFM*E*YNQKEPTRKYEI*STC*RAIENHLC
FSA*HRFGVCKQL*FCKKGK*LS*TSKR*SFYH
PKYGLQKPCQKTSTE*TRKSFLGNQSQCPTL*P
WNCENSEDKAADTTSKDVCLH*IGI*FF*RYR**
GNLLQCGRSRIVTNHPSRNQG*NQFGFCKKGCL
*IF*DGCNKY*TSSTQ***FEHH*EACS*EASR
KVSG
*SSIWV*E*NKRL*RLLRAILSE*HFNHSA
EGYHAT*PDKAPAGNG*TRSCVRTAWEPAF*QL
PFHHK*LFCP*GPAKSRTKHIRKSSINFTEK**
IPYKPESRRPFC*QV*GVCR*FYQ*K*RTRSGKV
IPF*MPIIR**VVHAQLLWESSE*KLPISRGAH
*GC*CGGATAGRVWATRFDGNILLAKARSRGNP
LPGIWNQPLL**P*I*SF*RQSPRVSSCWQHTIF
NLCIESSPIESCRICPESSCCSYY*YCWV*CNG
RKCEQGEARIDSFNRKGQQKNVHGGVWPDPRRI
YARVQVCQKTPHHFN*SNY*RDYSCCYENRC*VC
V*TDTEIFSRNCGRKMGS*LFLGDPVY*RKK

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSGSLEQKEMDLSALRVEEVQNVINAMQKILECP
ICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGP
SQCPLCKNDITKRSLQESTRFSQLVEELLKIIC
AFQLDTGLEYANSYNFAKKENNSPEHLKDEVSII
QSMGYRNRAKRLLQSEPENPSLETSLSVQLSNL
GTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVN
KATYCSVGDQELLQITPQGTRDEISLDSAKKAAC
EFSETDVTNTEHHQPSNNDLNTTEKRAAERHPE
KYQ
GEAASGCESETSVSEDCSGLSSQSDILTTQ
QRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSY
PSIISDSSALEDLRNPEQSTSEKAVLTSQKSSE
YPISQNPEGLSADKFEVSADSSTSKNKEPGVER
SSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELI
KVVDVEEQQLEESGPHDLTETSYLPRQDLEGTP
YLESGISLFSDDPESDPSEDRAPESARVGNIPS
STSALKVPQLKVAESAQSPAAAHTTDTAGYNAME
ESVSREKPELTASTERVNKRMSMVVSGLTPEEF
MLVYKFARKHHITLTNLITEETTHVVMKTDAEF
VCERTLKYFLGIAGGKWVVSYFWVTQSIKERK

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALVHWNRKKWIYLLFALKKYKMSLMLCRKS*SV
PSVWS*SRNLSPQSVTTYFANFAC*NFSTRRKGL
HSVLYVRMI*PKGAYKKVRDLVNLLKSY*KSFV
LFSLTQVWSMQTAIILQKRKITLLNI*KMKFLS
SKVWATETVPKDFYRVNPKILPWKPVSVSNSLTL
EL*EL*GQSSGYNLKRRLSTLNWDLILLKIPLI
RQLIAVWEIKNCYKSPLKEPGMKSVWILQKRLL
VNFLRRM*QILNIINPVIMI*TPLRSVQLRGIQK
SIR
VKQHLGVRVKQASLKTAQGYPLRVTF*PLS
RGIPCNIT**SSSRKWLN*KLC*NSMGASLLTA
TLPS*VTLLPLRTCEIQNKAHQKKQY*LHRKVVN
TL*ARIQKAFLLTSLRCLQIVLPVKIKNQEWKG
HPLLNAHH*MIGGTCTVALGVFRIETTHLKRSS
LRLLMWRSNSWKSLGHTI*RKHLTCQGKI*REPL
TWNLESASSLMTLNLILLKTEPQSQLVLATYHL
QPLH*KFPN*KLQNLPRVQLLLILLILLGIMQW
KKV*AGRSQN*QLQQKGSTKECPWWCLA*PQKNL
CSCTSLPENTTSL*LI*LLKRLLMLL*KQMLSL
CVNGH*NIF*ELREENG*LAISG*PSLLKKEK

Sequence BRCA1.oAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCC
CATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCT
TCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTG
CTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTCTATCAT
CCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTCTCTAAC
CTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATACCGTTA
ATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGC
TTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCA
GAAAAGTATCAGG
GTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTC
AGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAG
CTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGTATTAACTTCACAGAAAAGTAGTGAA
TACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAGGT
CATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAGCTCAT
TAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCT
TACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACCATCTT
CAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGA
AGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAGAATTT
ATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTG
TGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATGCT
GAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCTTCAGG
GGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGC
TTCCATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACC
TGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGC
CCTGGGAGCTCCTCTCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCC
TTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCA
CCAATTGAGCAAGATGCTGATTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAA
GAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATT
ATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAA
CTTGAAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGC
CAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGA
ATCACTTCAGCCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAA
AAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAAC
CGGTTTCCAAAGACAGTCTTCTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACC
TCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTG
AAACCATACAGCTTCATAAATAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTA
GGTCTTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGG
TAGCAAACACTTCCA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWFIGTERNGFICSSR*RSTKCH*CYAENLRVS
HLSGVDQGTCLHKV*PHILQILHAETSQPEERA
FTVSFM*E*YNQKEPTRKYEI*STC*RAIENHLC
FSA*HRFGVCKQL*FCKKGK*LS*TSKR*SFYH
PKYGLQKPCQKTSTE*TRKSFLAGNQSQCPTL*
PWNCENSEDKAADTTSKDVCLH*IGI*FF*RYR*
*GNLLQCGRSRIVTNHPSRNQG*NQFGFCKKGC
L*IF*DGCNKY*TSSTQ***FEHH*EACS*EAS
RKVSG
*SSIWV*E*NKRL*RLLRAILSE*HFNHS
AEGYHAT*PDKAPAGNG*TRSCVRTAWEPAF*Q
LPFHHK*LFCP*GPAKSRTKHIRKSINFTEK**
IPYKPESRRPFC*QV*GVCR*FYQ*K*RTRSGKV
IPF*MPIIR**VVHAQLLWESSE*KLPISRGAH
*GC*CGGATAGRVWATRFDGNILLAKARSRGNP
LPGIWNQPLL**P*I*SF*RQSPRVSSCWQHTIF
NLCIESSPIESCRICPESSCCSYY*YCWV*CNG
RKCEQGEARIDSFNRKGQQKNVHGGVWPDPRRI
YARVQVCQKTPHHFN*SNY*RDYSCCYENRC*VC
V*TDTEIFSRNCGRKMGS*LFLGDPVY*RKKNA
E*A*F*SQRRCGQWKKPPRSKASKRIPGQKDLQ
GARNLLLWALHQHAHRVSTQLWLCSQMPGQRTMA
SMQLGRCVRHLW*PESGCWTV*HSTSARSWTPT
*YPRSPTATTDCSQPQVQSHRTPRMSLQSGLSR
PWELLSLFSPSTVLATKYFMYISLKRTSGYARVP
*RFSA*SLPWKSAMSTKLW*FFT*EDFKTI*TP
PIEQDADSLFISPILSIQAVVGLGLEAQSGLAS
RE*LVSLSLLL*NPVFTKAESQTLQWKESAWDRL
CDLKSE*SLGSSQMLEWNIGEEILRQVLEMKRK
LETWAWWLTPVIPALWEAKVGRSLEVRSSKPAW
PTW*NPISTKNTEISRSWWWTPVIPATQVAKAGE
SLQPGRWRLQ*AKIIPRHSSLGDSETVAQKKKK
KKENETRRDF*KSEIYLLDF*RMCSKTAEDFQE
PVSKDSLLIPH***VKCLLL*LWYIIHSS*NIRP
LA*IFHIYKMTDPTRKEAVAFFEVIFFLCSLLL
KPYSFINNFAC*RKKKCFS*THYPGLFIAVGRT
RSSLAPPVCKGSEDLIVQNTFCKCCAVNTANKLG
SKHF

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSGSLEQKEMDLSALRVEEVQNVINAMQKILECP
ICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGP
SQCPLCKNDITKRSLQESTRFSQLVEELLKIIC
AFQLDTGLEYANSYNFAKKENNSPEHLKDEVSII
QSMGYRNRAKRLLQSEPENPSLQETSLSVQLSN
LGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTV
NKATYCSVGDQELLQITPQGTRDEISLDSAKKAA
CEFSETDVTNTEHHQPSNNDLNTTEKRAAERHP
EKYQ
GEAASGCESETSVSEDCSGLSSQSDILTT
QQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNS
YPSIISDSSALEDLRNPEQSTSEKVLTSQKSSE
YPISQNPEGLSADKFEVSADSSTSKNKEPGVER
SSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELI
KVVDVEEQQLEESGPHDLTETSYLPRQDLEGTP
YLESGISLFSDDPESDPSEDRAPESARVGNIPS
STSALKVPQLKVAESAQSPAAAHTTDTAGYNAME
ESVSREKPELTASTERVNKRMSMVVSGLTPEEF
MLVYKFARKHHITLTNLITEETTHVVMKTDAEF
VCERTLKYFLGIAGGKWVVSYFWVTQSIKERKML
NEHDFEVRGDVVNGRNHQGPKRARESQDRKIFR
GLEICCYGPFTNMPTGCPPNCGCAARCLDRGQW
LPCNWADV*GTCGDPRVGVGQCSTLPVPGAGHLP
DTPDPPQPLLTAASHRYRATGPQE*AYKVAFPG
PGSSSHSSVLLLSWLLNILCTSA*KGLLAMQGS
LKDFLLEVSLGNLP*AQNYGNFSPEKILKPFKRH
QLSKMLIHYLSALFFLFRLLLA*GWKHRVAWPQ
ENSWFP*VYFSKTLCSQRQRVRPFNGRRVLGID
YVT*SQNSPWAVLKCWSGTLGRKF*GRY*K*KGN
LKPGHGGSRL*SQHFGRPRWADHWRSGVRNQPG
QHGETPSLLKIQKLAGHGGGHL*SQLLRWLRQE
NHFSPGGGGCSEPRSYHGTPAWVTVRLWLKKKKK
KRKMKLEEISKSLRYIC*ISKECVLKQQKIFKN
RFPKTVF*FLISNK*NVYCCSSGI*SIPLKI*D
LWHEYFISIK*QIPPGRKLLLSLR*FFSFAPCC*
NHTAS*IILLAEGRKSVFHKPIIQDCL*LLEGL
GLP*PPQCARAVKT*LYKIRFVNVVLLTLQINL
VANTS

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALVHWNRKKWIYLLFALKKYKMSLMLCRKS*SV
PSVWS*SRNLSPQSVTTYFANFAC*NFSTRRKGL
HSVLYVRMI*PKGAYKKVRDLVNLLKSY*KSFV
LFSLTQVWSMQTAIILQKRKITLLNI*KMKFLS
SKVWATETVPKDFYRVNPKILPCRKPVSVSNSLT
LEL*EL*GQSSGYNLKRRLSTLNWDLILLKIPL
IRQLIAVWEIKNCYKSPLKEPGMKSVWILQKRL
LVNFLRRM*QILNIINPVIMI*TPLRSVQLRGIQ
KSIR
VKQHLGVRVKQASLKTAQGYPLRVTF*PL
SRGIPCNIT**SSSRKWLN*KLC*NSMGASLLT
ATLPS*VTLLPLRTCEIQNKAHQKKY*LHRKVVN
TL*ARIQKAFLLTSLRCLQIVLPVKIKNQEWKG
HPLLNAHH*MIGGTCTVALGVFRIETTHLKRSS
LRLLMWRSNSWKSLGHTI*RKHLTCQGKI*REPL
TWNLESASSLMTLNLILLKTEPQSQLVLATYHL
QPLH*KFPN*KLQNLPRVQLLLILLILLGIMQW
KKV*AGRSQN*QLQQKGSTKECPWWCLA*PQKNL
CSCTSLPENTTSL*LI*LLKRLLMLL*KQMLSL
CVNGH*NIF*ELREENG*LAISG*PSLLKKEKC
*MSMILKSEEMWSMEETTKVQSEQENPRTERSSG
G*KSVAMGPSPTCPQGVHPIVVVQPDAWTEDNG
FHAIGQMCEAPVVTREWVLDSVALYQCQELDTY
LIPQIPHSHY*LQPATGTEPQDPKNELTKWPFQA
LGAPLTLQSFYCPGY*IFYVHQPEKDFWLCKGP
LKIFCLKSPLEICHEHKIMVIFHLRRF*NHLNA
TN*ARC*FIIYQPYSFYSGCCWLRAGSTEWLGLK
RIAGFPKFTSLKPCVHKGRESDPSMEGECLGSI
M*LKVRIVLGQFSNVGVEHWGGNSEAGIRNEKE
T*NLGMVAHACNPSTLGGQGGQITGGQEFETSLA
NMVKPHLY*KYRN*PVMVVDTCNPSYSGG*GRR
ITSAREVEVAVSQDHTTALQPG*Q*DCGSKKKK
KKGK*N*KRFLKV*DIFARFLKNVF*NSRRFSRT
GFQRQSSNSSLVISKMFIVVALVYNPFLLKYKT
SGMNISYL*NDRSHQEGSCCFL*GDFFPLLPVA
ETIQLHK*FCLLKEEKVFFINPLSRTVYSCWKD*
VFPSPPSVQGQ*RLDCTKYVL*MLCC*HCK*TW
*QTLP

Sequence BRCA1.lAug05

GTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGC
GACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCT
GCTCTGGGTAAAGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATGCTATGCAGAAAATCTTAGA
GTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAA
GGGCCTTCACAGTGTCCTTTATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCA
TTTGTGCTTTTCAGCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACATCTAAAAGATGAAGTTTC
TATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAGGAAACCAGTCTCAGTGTCCAACTC
TCT
AACCTTGGAACTGTGAGAACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAAGATA
CCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGAAATCAGTTTGGATTCTGCAAAAAA
GGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGG
CATCCAGAAAAGTATCAGG
GTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAA
CCACTCAGCAGAGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTC
TAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGCACATCAGAAAAAGTATTAACTTCACAGAAAAGT
AGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGG
AAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGA
GCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGA
ACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATAC
CATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCCAGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGC
AATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAA
GAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTG
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAA
AATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATC
TTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGG
AGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGAT
GTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCAC
AGCCACTACTGACTGCAGCCAGCCACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTCTCACTC
TTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTGGCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAA
GTCTCCCTTGGAAATCTGCCATGAGCACAAAATTATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGA
TTCATTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGGCCTCAAGAGAATAGCTGGTTTCCCTA
AGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCAGACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAAT
AGTCCTTGGGCAGTTCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTGAAACCTGGGCATGGTG
GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATC
TCTACTAAAAATACAGAAATTAGCCGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCGGGAGGT
GGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGCTCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAG
AAGAGATTTCTAAAAGTCTGAGATATATTTGCTAGATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTT
CTAATTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATATAAGACCTCTGGCATGAATATTTCATA
TCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTCTTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAA
TAATTTTGCTTGCTGAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTCTTCCCTAGCCCCCCCA
GTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGTGCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCA
  • BRCA1-F2 (forward primer)
  • BRCA1-R2 (reverse primer)
  • Single exon skipping

Frame 0

VP*FRILRGCCLAVAPWFPWQRKSAGITDKLKL
RLRGVSSLRLPGRGTGCGVSQITGPLRSGGLHPL
LWVKFIGTERNGFICSSR*RSTKCH*CYAENLR
VSHLSGVDQGTCLHKV*PHILQILHAETSQPEE
RAFTVSFM*E*YNQKEPTRKYEI*STC*RAIENH
LCFSA*HRFGVCKQL*FCKKGK*LS*TSKR*SF
YHPKYGLQKPCQKTSTE*TRKSFLAGNQSQCPT
L
*PWNCENSEDKAADTTSKDVCLH*IGI*FF*RY
R**GNLLQCGRSRIVTNHPSRNQG*NQFGFCKK
GCL*IF*DGCNKY*TSSTQ***FEHH*EACS*E
ASRKVSG
*SSIWV*E*NKRL*RLLRAILSE*HFN
HSAEGYHAT*PDKAPAGNG*TRSCVRTAWEPAF
*QLPFHHK*LFCP*GPAKSRTKHIRKSINFTEK
**IPYKPESRRPFC*QV*GVCR*FYQ*K*RTRSG
KVIPF*MPIIR**VVHAQLLWESSE*KLPISRG
AH*GC*CGGATAGRVWATRFDGNILLAKARSRG
NPLPGIWNQPLL**P*I*SF*RQSPRVSSCWQHT
IFNLCIESSPIESCRICPESSCCSYY*YCWV*C
NGRKCEQGEARIDSFNRKGQQKNVHGGVWPDPR
RIYARVQVCQKTPHHFN*SNY*RDYSCCYENRC*
VCV*TDTEIFSRNCGRKMGS*LFLGDPVY*RKK
NAE*A*F*SQRRCGQWKKPPRSKASKRIPGQKD
LQGARNLLLWALHQHAHRSTGMDGTAVWCFCGEG
AFIIHPWHRCPPNCGCAARCLDRGQWLPCNWAD
V*GTCGDPRVGVGQCSTLPVPGAGHLPDTPDPP
QPLLTAASHRYRATGPQE*AYKVAFPGPGSSSHS
SVLLLSWLLNILCTSA*KGLLAMQGSLKDFLLE
VSLGNLP*AQNYGNFSPEKILKPFKRHQLSKML
IHYLSALFFLFRLLLA*GWKHRVAWPQENSWFP*
VYFSKTLCSQRQRVRPFNGRRVLGIDYVT*SQN
SPWAVLKCWSGTLGRKF*GRY*K*KGNLKPGHG
GSRL*SQHFGRPRWADHWRSGVRNQPGQHGETPS
LLKIQKLAGHGGGHL*SQLLRWLRQENHFSPGG
GGCSEPRSYHGTPAWVTVRLWLKKKKKKRKMKL
EEISKSLRYIC*ISKECVLKQQKIFKNRFPKTVF
*FLISNK*NVYCCSSGI*SIPLKI*DLWHEYFI
SIK*QIPPGRKLLLSLR*FFSFAPCC*NHTAS*
IILLAEGRKSVFHKPIIQDCL*LLEGLGLP*PPQ
CARAVKT*LYKIRFVNVVLLTLQINLVANTS

Frame 1

YLDFVF*EAAA*R*PLGFRGNGKARELQIN*NC
DCAA*AR*DFLDGGQAVGFLR*LGPCAQEAFTL
CSG*SSLEQKEMDLSALRVEEVQNVINAMQKILE
CPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK
GPSQCPLCKNDITKRSLQESTRFSQLVEELLKI
ICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVS
IIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL
S
NLGTVRTLRTKQRIQPQKTSVYIELGSDSSED
TVNKATYCSVGDQELLQITPQGTRDEISLDSAKK
AACEFSETDVTNTEHHQPSNNDLNTTEKRAAER
HPEKYQ
GEAASGCESETSVSEDCSGLSSQSDIL
TTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPS
NSYPSIISDSSALEDLRNPEQSTSEKVLTSQKS
SEYPISQNPEGLSADKFEVSADSSTSKNKEPGV
ERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEE
LIKVVDVEEQQLEESGPHDLTETSYLPRQDLEG
TPYLESGISLFSDDPESDPSEDRAPESARVGNI
PSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNA
MEESVSREKPELTASTERVNKRMSMVVSGLTPE
EFMLVYKFARKHHITLTNLITEETTHVVMKTDA
EFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERK
MLNEHDFEVRGDVVNGRNHQGPKRARESQDRKI
FRGLEICCYGPFTNMPTDQLEWMVQLCGASVVK
ELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQM
CEAPVVTREWVLDSVALYQCQELDTYLIPQIPH
SHY*LQPATGTEPQDPKNELTKWPFQALGAPLT
LQSFYCPGY*IFYVHQPEKDFWLCKGPLKIFCLK
SPLEICHEHKIMVIFHLRRF*NHLNATN*ARC*
FIIYQPYSFYSGCCWLRAGSTEWLGLKRIAGFP
KFTSLKPCVHKGRESDPSMEGECLGSIM*LKVRI
VLGQFSNVGVEHWGGNSEAGIRNEKET*NLGMV
AHACNPSTLGGQGGQITGGQEFETSLANMVKPH
LY*KYRN*PVMVVDTCNPSYSGG*GRRITSAREV
EVAVSQDHTTALQPG*Q*DCGSKKKKKKGK*N*
KRFLKV*DIFARFLKNVF*NSRRFSRTGFQRQS
SNSSLVISKMFIVVALVYNPFLLKYKTSGMNISY
L*NDRSHQEGSCCFL*GDFFPLLPVAETIQLHK
*FCLLKEEKVFFINPLSRTVYSCWKD*VFPSPP
SVQGQ*RLDCTKYVL*MLCC*HCK*TW*QTLP

Frame 2

TLISYSERLLLSGSPLVSVATEKRGNYR*IKTA
TARRELAETSWTGDRLWGFSDNWAPALRRPSPS
ALGKVHWNRKKWIYLLFALKKYKMSLMLCRKS*
SVPSVWS*SRNLSPQSVTTYFANFAC*NFSTRRK
GLHSVLYVRMI*PKGAYKKVRDLVNLLKSY*KS
FVLFSLTQVWSMQTAIILQKRKITLLNI*KMKF
LSSKVWATETVPKDFYRVNPKILPCRKPVSVSNS
L
TLEL*EL*GQSSGYNLKRRLSTLNWDLILLKI
PLIRQLIAVWEIKNCYKSPLKEPGMKSVWILQK
RLLVNFLRRM*QILNIINPVIMI*TPLRSVQLRG
IQKSIR
VKQHLGVRVKQASLKTAQGYPLRVTF*
PLSRGIPCNIT**SSSRKWLN*KLC*NSMGASL
LTATLPS*VTLLPLRTCEIQNKAHQKKY*LHRKV
VNTL*ARIQKAFLLTSLRCLQIVLPVKIKNQEW
KGHPLLNAHH*MIGGTCTVALGVFRIETTHLKR
SSLRLLMWRSNSWKSLGHTI*RKHLTCQGKI*RE
PLTWNLESASSLMTLNLILLKTEPQSQLVLATY
HLQPLH*KFPN*KLQNLPRVQLLLILLILLGIM
QWKKV*AGRSQN*QLQQKGSTKECPWWCLA*PQK
NLCSCTSLPENTTSL*LI*LLKRLLMLL*KQML
SLCVNGH*NIF*ELREENG*LAISG*PSLLKKE
KC*MSMILKSEEMWSMEETTKVQSEQENPRTERS
SGG*KSVAMGPSPTCPQINWNGWYSCVVLLW*R
SFHHSPLAQVSTQLWLCSQMPGQRTMASMQLGR
CVRHLW*PESGCWTV*HSTSARSWTPT*YPRSPT
ATTDCSQPQVQSHRTPRMSLQSGLSRPWELLSL
FSPSTVLATKYFMYISLKRTSGYARVP*RFSA*
SLPWKSAMSTKLW*FFT*EDFKTI*TPPIEQDAD
SLFISPILSIQAVVGLGLEAQSGLASRE*LVSL
SLLL*NPVFTKAESQTLQWKESAWDRLCDLKSE
*SLGSSQMLEWNIGEEILRQVLEMKRKLETWAWW
LTPVIPALWEAKVGRSLEVRSSKPAWPTW*NPI
STKNTEISRSWWWTPVIPATQVAKAGESLQPGR
WRLQ*AKIIPRHSSLGDSETVAQKKKKKKENETR
RDF*KSEIYLLDF*RMCSKTAEDFQEPVSKDSL
LIPH***VKCLLL*LWYIIHSS*NIRPLA*IFH
IYKMTDPTRKEAVAFFEVIFFLCSLLLKPYSFIN
NFAC*RKKKCFS*THYPGLFIAVGRTRSSLAPP
VCKGSEDLIVQNTFCKCCAVNTANKLGSKHF

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