Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence BCL2L1.dAug05

GGCTGCGCTGGGGCGGACCGCCGGGGGCGAGGCCCCTCCTCTCAGGAAGGTCTAGGGGGCGCCCCCGGGGGAGGGCGGTTGCCTAGCAACGGGGCGGTGG CTCGGCGGGTACCCGGCCTAGTGAGCTCGAGAAGCCGCTAGGCCGCAGCCGGCCCACCCTGGGCTCCGGAGAGGTGCAGCCCCCGGCCCCGGAGCCCTCC CGGGGGCGCCAGAAAGCCACTTTGGGCCTGTCCCCAGGAAAACGTGGTCTCAGCTGTGGGGCCGTCGAGCAGGCCTCGACCCCTCTTCCTGACCTGAGGA GAGGCCCAGCGCCTCACCCTCACCCAGTCTTTGTGCAGGGCGCCGGCGGCCATTGTGTCCGGGCGGGGAATGGAGGACCCGGCAATCCCCAACTGCCACG TTCTGGGCCTTTGGGGAATTCAGAGCAAACCAGCGGCATTTGTTGGGGGTCTCCGGCCTTCAACATCACAGACAGGCCTGGGGGTGGCCTTCCCAAAGTC AGATTGCAGATCTGAGGCAGTTTCCCCCTCCCTGCGTCCCTCACTGAAACCTTGAACCCCATTGAGAAGTCCCTTTAGGGTTTCGGACGCCTCCACCTCA CCCTGGGCTGGTGCTTAAATAGAAAAAAGAAAAACAAAAACCAACTAAATCCATACCAGCCACCTCCGGGAGAGTACTCCTGGCTCCCAGTAGGAGGCGG AGAGCCAAGGGGCGTGCAAGAGAGAGGGGGCTGGGCTCCCGGGTGGCAGGAGGCCGCGGCTGCGGAGCGGCCGCCCTCGATCCGGGCGATGGAGGAGGAA GCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTT TTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGCACCTGCC TGCCTTTGCCTAAGGCGGATTTGAATCTCTTTCTCTCCCTTCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCA GTGAGTGAGCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTA CAAGCTTTCCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCC AGTGCCATCAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCC CCATGGCAGCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCAC CCCAGGGACAGCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGG GCACTGTGCGTGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGA TCCAGGAGAACGGCGGCTGGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCT GACGGGCATGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCT GCTCCACCACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGT CCCTTGCAGCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCACAAAATCTGTC CAAAGGAGGCTGGCAGGTATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCTGAAAATGTTTT TCTGCCAGGGAGCTTGAAAGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTGATCATTTTCCC CCCACTCTCCCCACACTAACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCAGGCTGCTTGGG ATAAAGATGCAAGGACCAGGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGGCTAGAGGCCAG CCCCACCCAGGAGGGAGGGGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCCCTTCCCCTCCC TGGCTCCCATGACCATACTGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAAGAGCAGCCTGT GGCATCTTTGCCTTGGGCTGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGCCCCAGGGTCTT CCCTACCTCAGGCAGGAAGGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGGCAGATCTGTGC CCCATGCCTGTCCAGCCTGGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCCACCCTTGGCCT GTCTCATCCCCAGGGGTCCCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTATTTTTGTTAAGC GTGTCTGTATTTATGTGTGAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTCCCTCCCCTGCC CTGGTCCAGGGAAGCTGGCCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTTGAAATAGTCTG TGTGAAGGTGAAAGTGCAGTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCLX-3 (forward primer)
BCLX-2 (reverse primer)
Single exon skipping

Frame 0

GCAGADRRGRGPSSQEGLGGAPGGGRLPSNGAV ARRVPGLVSSRSR*AAAGPPWAPERCSPRPRSPP GGARKPLWACPQENVVSAVGPSSRPRPLFLT*G EAQRLTLTQSLCRAPAAIVSGRGMEDPAIPNCH VLGLWGIQSKPAAFVGGLRPSTSQTGLGVAFPKS DCRSEAVSPSLRPSLKP*TPLRSPFRVSDASTS PWAGA*IEKRKTKTN*IHTSHLRESTPGSQ*EA ESQGACKREGAGLPGGRRPRLRSGRPRSGRWRRK QARGLVPELRNSCVAFWAPSLPGRMIPPAGAGF FASHREAG*VTGIPAATSSPDL*YKRSSGGCTC LPLPKADLNLFLSLQNLILALDLRRESLTRDETQ *VSRCFGQWTG*AHPYYKNVSEQPGAGG*LSLL QAFPERIQLESV**CGREQD*GPRRD*IGDGDP QCHQWQPILAPGRQPRGEWSHWPQQQFGCPGGDP HGSSKASAEGGRRRV*TAVPAGIQ*PDIPAPHH PRDSISEL*TGSE*TLPGWGKLGSHCGLFLLRR GTVRGKRRQGDAGIGESDRSLDGHLPE*PPRALD PGERRLGYFCGTLWEQCSSREPKGPGTLQPLVP DGHDCGRRGSAGLTLQSEMTRH*PSTLPSHPLL CSTTSSVQPPLPPGEPLHAAHAHLPITGLGPDLV PCS*FSRIYHTSVRPPHLSSLGLRIHKISTKSV QRRLAGMEGFVAGGRRALPDWCNPYPLASLKMF FCQGA*KFSEPLPQKGD*IAFVLMFVASELIIFP PLSPH*PGFPFLPSLPPKSHLGATFD*GFRLLG IKMQGPGLPPHLWTG*SPHSQSKCPPEASG*RP APPRREGAIATGSTPCQS*GGPCSSAPP*SLPLP GSHDHTEGPTGPKTDAPELFMASAASLPTRAAC GIFALGCSSWWVQGTQP*GERELSGTAMGAPGS SLPQAGRAGRRACCMGWGRAD*KGQSCLARQICA PCLSSLGSQAAKARVAWPGALQASLSLLLHPWP VSSPGVPATPGSLLYIFETSFYSL*R*YTIFVK RVCIYV*GAAGLQCACTWRAGARRLDGLMLPPLP WSREAGRGSWLLRGICPSPNPHPTLVPAL*NSL CEGESAVQ**TVFTQ*TKS

Frame 1

AALGRTAGGEAPPLRKV*GAPPGEGGCLATGRW LGGYPA**AREAARPQPAHPGLRRGAAPGPGAL PGAPESHFGPVPRKTWSQLWGRRAGLDPSS*PEE RPSASPSPSLCAGRRRPLCPGGEWRTRQSPTAT FWAFGEFRANQRHLLGVSGLQHHRQAWGWPSQS QIADLRQFPPPCVPH*NLEPH*EVPLGFRTPPPH PGLVLK*KKEKQKPTKSIPATSGRVLLAPSRRR RAKGRARERGLGSRVAGGRGCGAAALDPGDGGG SKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVF LPATARPAELPASPQPPPLPTCDTKDLPGAAPA CLCLRRI*ISFSPFRILSWLWILEENH*PETRL SE*AGVLDNGLVEPIPIIKMSQSNRELVVDFLSY KLSQKGYSWSQFSDVEENRTEAPEGTESEMETP SAINGNPSWHLADSPAVNGATGHSSSLDAREVI PMAAVKQALREAGDEFELRYRRAFSDLTSQLHIT PGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGG ALCVESVDKEMQVLVSRIAAWMATYLNDHLEPW IQENGGWDTFVELYGNNAAAESRKGQERFNRWFL TGMTVAGVVLLGSLFSRK*PDTDHPLYPPTPFS APPHPPSSRHCHQENHYMQPMPTCPSQGWAQIW SLAASFLEFITLL*DPHTSVPLASEFTKFPQNLS KGGWQVWKGLWLGAGGPYLIGATLTP*PP*KCF SARELESFQNLFPRKETRLPLF*CLWPQN*SFS PHSPHTNLGSLSFHPYPLRAI*GPLLTRDSGCLG *RCKDQDSLLTSGLARVLTPSPNVLQKPLARGQ PHPGGRGL*LQEAPHAKARVALAVQHHPSPFPS LAPMTILRDQLGPRQMPQSCLWPQLPHFLQEQPV ASLPWAAPHGGFRGLSPEVKGSYQEQLWEPQGL PYLRQEGQEGEPAAWGGVGLTRRASPAWPGRSV PHACPAWAARLPRPEWPGQELFRPPSLFCSTLGL SHPQGSQPPRALCCTYLRLVFIPCEDDILFLLS VSVFMCEELLACSARARGELVPGDWTA*CSLPC PGPGKLAEGPGS*GASAPPPTPTPHLFQLFEIVC VKVKVQFSNKLCLLSEQRA

Frame 2

LRWGGPPGARPLLSGRSRGRPRGRAVA*QRGGG SAGTRPSELEKPLGRSRPTLGSGEVQPPAPEPS RGRQKATLGLSPGKRGLSCGAVEQASTPLPDLR RGPAPHPHPVFVQGAGGHCVRAGNGGPGNPQLPR SGPLGNSEQTSGICWGSPAFNITDRPGGGLPKV RLQI*GSFPLPASLTETLNPIEKSL*GFGRLHL TLGWCLNRKKKNKNQLNPYQPPPGEYSWLPVGGG EPRGVQERGGWAPGWQEAAAAERPPSIRAMEEE ASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWF FCQPPRGRLSYRHPRSHLLSRPVIQKIFRGLHLP AFA*GGFESLSLPSESYLGFGS*KRITNQRRDS VSEQVFWTMDWLSPSLL*KCLRATGSWWLTFSP TSFPRKDTAGVSLVMWKRTGLRPQKGLNRRWRPP VPSMATHPGTWQTAPR*MEPLATAAVWMPGR*S PWQQ*SKR*GRQATSLNCGTGGHSVT*HPSSTS PQGQHIRALNR**MNSSGMG*TGVALWPFSPSAG HCAWKA*TRRCRYW*VGSQLGWPLT*MTT*SLG SRRTAAGILLWNSMGTMQQPRAERARNASTAGS *RA*LWPAWFCWAHSSVGNDQTLTIHSTLPPPSL LHHILRPAAIATRRTTTCSPCPPAHHRVGPRSG PLQLVF*NLSHFCETPTPQFPWPQNSQNFHKIC PKEAGRYGRVCGWGQEGPT*LVQPLPLSLPENVF LPGSLKVFRTSSPERRLDCLCFDVCGLRIDHFP PTLPTLTWVPFPSIPTP*EPFRGHF*LGIQAAW DKDARTRTPSSPLDWLESSLPVQMSSRSLWLEAS PTQEGGGYSYRKHPMPKLGWPLQFSTTLVPSPP WLP*PY*GTNWAQDRCPRAVYGLSCLTSYKSSL WHLCLGLLLMVGSGDSALR*KGAIRNSYGSPRVF PTSGRKGRKESLLHGVG*G*LEGPVLPGQADLC PMPVQPGQPGCQGQSGLARSSSGLPLSSAPPLA CLIPRGPSHPGLSAVHI*D*FLFLVKMIYYFC*A CLYLCVRSCWLAVRVHVESWCPEIGRPDAPSPA LVQGSWPRVLAPEGHLPLPQPPPHTCSSSLK*S V*R*KCSSVINCVYSVNKE

Sequence BCL2L1.cAug05

GGCTGCGCTGGGGCGGACCGCCGGGGGCGAGGCCCCTCCTCTCAGGAAGGTCTAGGGGGCGCCCCCGGGGGAGGGCGGTTGCCTAGCAACGGGGCGGTGG CTCGGCGGGTACCCGGCCTAGTGAGCTCGAGAAGCCGCTAGGCCGCAGCCGGCCCACCCTGGGCTCCGGAGAGGTGCAGCCCCCGGCCCCGGAGCCCTCC CGGGGGCGCCAGAAAGCCACTTTGGGCCTGTCCCCAGGAAAACGTGGTCTCAGCTGTGGGGCCGTCGAGCAGGCCTCGACCCCTCTTCCTGACCTGAGGA GAGGCCCAGCGCCTCACCCTCACCCAGTCTTTGTGCAGGGCGCCGGCGGCCATTGTGTCCGGGCGGGGAATGGAGGACCCGGCAATCCCCAACTGCCACG TTCTGGGCCTTTGGGGAATTCAGAGCAAACCAGCGGCATTTGTTGGGGGTCTCCGGCCTTCAACATCACAGACAGGCCTGGGGGTGGCCTTCCCAAAGTC AGATTGCAGATCTGAGGCAGTTTCCCCCTCCCTGCGTCCCTCACTGAAACCTTGAACCCCATTGAGAAGTCCCTTTAGGGTTTCGGACGCCTCCACCTCA CCCTGGGCTGGTGCTTAAATAGAAAAAAGAAAAACAAAAACCAACTAAATCCATACCAGCCACCTCCGGGAGAGTACTCCTGGCTCCCAGTAGGAGGCGG AGAGCCAAGGGGCGTGCAAGAGAGAGGGGGCTGGGCTCCCGGGTGGCAGGAGGCCGCGGCTGCGGAGCGGCCGCCCTCGATCCGGGCGATGGAGGAGGAA GCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTT TTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGCACCTGCC TGCCTTTGCCTAAGGCGGATTTGAATAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGAGCAGGTGTTT TGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTTCCCAGAAAGG ATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCATCAATGGCAAC CCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCAGCAGTAAAGC AAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGACAGCATATCA GAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTGCGTGGAAAGC GTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAGAACGGCGGCT GGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCTGACGGGCATGACTGTGGC CGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCTGCTCCACCACATCCTCCG TCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGTCCCTTGCAGCTAGTTTTC TAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCACAAAATCTGTCCAAAGGAGGCTGGCAGGT ATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCTGAAAATGTTTTTCTGCCAGGGAGCTTGAA AGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTGATCATTTTCCCCCCACTCTCCCCACACTA ACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCAGGCTGCTTGGGATAAAGATGCAAGGACCA GGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGGCTAGAGGCCAGCCCCACCCAGGAGGGAGG GGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCCCTTCCCCTCCCTGGCTCCCATGACCATAC TGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAAGAGCAGCCTGTGGCATCTTTGCCTTGGGC TGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGCCCCAGGGTCTTCCCTACCTCAGGCAGGAA GGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGGCAGATCTGTGCCCCATGCCTGTCCAGCCT GGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCCACCCTTGGCCTGTCTCATCCCCAGGGGTC CCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTATTTTTGTTAAGCGTGTCTGTATTTATGTGT GAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTCCCTCCCCTGCCCTGGTCCAGGGAAGCTGG CCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTTGAAATAGTCTGTGTGAAGGTGAAAGTGCA GTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCLX-3 (forward primer)
BCLX-2 (reverse primer)
Single exon skipping

Frame 0

GCAGADRRGRGPSSQEGLGGAPGGGRLPSNGAV ARRVPGLVSSRSR*AAAGPPWAPERCSPRPRSPP GGARKPLWACPQENVVSAVGPSSRPRPLFLT*G EAQRLTLTQSLCRAPAAIVSGRGMEDPAIPNCH VLGLWGIQSKPAAFVGGLRPSTSQTGLGVAFPKS DCRSEAVSPSLRPSLKP*TPLRSPFRVSDASTS PWAGA*IEKRKTKTN*IHTSHLRESTPGSQ*EA ESQGACKREGAGLPGGRRPRLRSGRPRSGRWRRK QARGLVPELRNSCVAFWAPSLPGRMIPPAGAGF FASHREAG*VTGIPAATSSPDL*YKRSSGGCTC LPLPKADLNNLILALDLRRESLTRDETQ*VSRCF GQWTG*AHPYYKNVSEQPGAGG*LSLLQAFPER IQLESV**CGREQD*GPRRD*IGDGDPQCHQWQ PILAPGRQPRGEWSHWPQQQFGCPGGDPHGSSKA SAEGGRRRV*TAVPAGIQ*PDIPAPHHPRDSIS EL*TGSE*TLPGWGKLGSHCGLFLLRRGTVRGK RRQGDAGIGESDRSLDGHLPE*PPRALDPGERRL GYFCGTLWEQCSSREPKGPGTLQPLVPDGHDCG RRGSAGLTLQSEMTRH*PSTLPSHPLLCSTTSS VQPPLPPGEPLHAAHAHLPITGLGPDLVPCS*FS RIYHTSVRPPHLSSLGLRIHKISTKSVQRRLAG MEGFVAGGRRALPDWCNPYPLASLKMFFCQGA* KFSEPLPQKGD*IAFVLMFVASELIIFPPLSPH* PGFPFLPSLPPKSHLGATFD*GFRLLGIKMQGP GLPPHLWTG*SPHSQSKCPPEASG*RPAPPRRE GAIATGSTPCQS*GGPCSSAPP*SLPLPGSHDHT EGPTGPKTDAPELFMASAASLPTRAACGIFALG CSSWWVQGTQP*GERELSGTAMGAPGSSLPQAG RAGRRACCMGWGRAD*KGQSCLARQICAPCLSSL GSQAAKARVAWPGALQASLSLLLHPWPVSSPGV PATPGSLLYIFETSFYSL*R*YTIFVKRVCIYV *GAAGLQCACTWRAGARRLDGLMLPPLPWSREAG RGSWLLRGICPSPNPHPTLVPAL*NSLCEGESA VQ**TVFTQ*TKS

Frame 1

AALGRTAGGEAPPLRKV*GAPPGEGGCLATGRW LGGYPA**AREAARPQPAHPGLRRGAAPGPGAL PGAPESHFGPVPRKTWSQLWGRRAGLDPSS*PEE RPSASPSPSLCAGRRRPLCPGGEWRTRQSPTAT FWAFGEFRANQRHLLGVSGLQHHRQAWGWPSQS QIADLRQFPPPCVPH*NLEPH*EVPLGFRTPPPH PGLVLK*KKEKQKPTKSIPATSGRVLLAPSRRR RAKGRARERGLGSRVAGGRGCGAAALDPGDGGG SKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVF LPATARPAELPASPQPPPLPTCDTKDLPGAAPA CLCLRRI*IILSWLWILEENH*PETRLSE*AGV LDNGLVEPIPIIKMSQSNRELVVDFLSYKLSQKG YSWSQFSDVEENRTEAPEGTESEMETPSAINGN PSWHLADSPAVNGATGHSSSLDAREVIPMAAVK QALREAGDEFELRYRRAFSDLTSQLHITPGTAYQ SFEQVVNELFRDGVNWGRIVAFFSFGGALCVES VDKEMQVLVSRIAAWMATYLNDHLEPWIQENGG WDTFVELYGNNAAAESRKGQERFNRWFLTGMTVA GVVLLGSLFSRK*PDTDHPLYPPTPFSAPPHPP SSRHCHQENHYMQPMPTCPSQGWAQIWSLAASF LEFITLL*DPHTSVPLASEFTKFPQNLSKGGWQV WKGLWLGAGGPYLIGATLTP*PP*KCFSARELE SFQNLFPRKETRLPLF*CLWPQN*SFSPHSPHT NLGSLSFHPYPLRAI*GPLLTRDSGCLG*RCKDQ DSLLTSGLARVLTPSPNVLQKPLARGQPHPGGR GL*LQEAPHAKARVALAVQHHPSPFPSLAPMTI LRDQLGPRQMPQSCLWPQLPHFLQEQPVASLPWA APHGGFRGLSPEVKGSYQEQLWEPQGLPYLRQE GQEGEPAAWGGVGLTRRASPAWPGRSVPHACPA WAARLPRPEWPGQELFRPPSLFCSTLGLSHPQGS QPPRALCCTYLRLVFIPCEDDILFLLSVSVFMC EELLACSARARGELVPGDWTA*CSLPCPGPGKL AEGPGS*GASAPPPTPTPHLFQLFEIVCVKVKVQ FSNKLCLLSEQRA

Frame 2

LRWGGPPGARPLLSGRSRGRPRGRAVA*QRGGG SAGTRPSELEKPLGRSRPTLGSGEVQPPAPEPS RGRQKATLGLSPGKRGLSCGAVEQASTPLPDLR RGPAPHPHPVFVQGAGGHCVRAGNGGPGNPQLPR SGPLGNSEQTSGICWGSPAFNITDRPGGGLPKV RLQI*GSFPLPASLTETLNPIEKSL*GFGRLHL TLGWCLNRKKKNKNQLNPYQPPPGEYSWLPVGGG EPRGVQERGGWAPGWQEAAAAERPPSIRAMEEE ASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWF FCQPPRGRLSYRHPRSHLLSRPVIQKIFRGLHLP AFA*GGFE*SYLGFGS*KRITNQRRDSVSEQVF WTMDWLSPSLL*KCLRATGSWWLTFSPTSFPRK DTAGVSLVMWKRTGLRPQKGLNRRWRPPVPSMAT HPGTWQTAPR*MEPLATAAVWMPGR*SPWQQ*S KR*GRQATSLNCGTGGHSVT*HPSSTSPQGQHI RALNR**MNSSGMG*TGVALWPFSPSAGHCAWKA *TRRCRYW*VGSQLGWPLT*MTT*SLGSRRTAA GILLWNSMGTMQQPRAERARNASTAGS*RA*LW PAWFCWAHSSVGNDQTLTIHSTLPPPSLLHHILR PAAIATRRTTTCSPCPPAHHRVGPRSGPLQLVF *NLSHFCETPTPQFPWPQNSQNFHKICPKEAGR YGRVCGWGQEGPT*LVQPLPLSLPENVFLPGSLK VFRTSSPERRLDCLCFDVCGLRIDHFPPTLPTL TWVPFPSIPTP*EPFRGHF*LGIQAAWDKDART RTPSSPLDWLESSLPVQMSSRSLWLEASPTQEGG GYSYRKHPMPKLGWPLQFSTTLVPSPPWLP*PY *GTNWAQDRCPRAVYGLSCLTSYKSSLWHLCLG LLLMVGSGDSALR*KGAIRNSYGSPRVFPTSGRK GRKESLLHGVG*G*LEGPVLPGQADLCPMPVQP GQPGCQGQSGLARSSSGLPLSSAPPLACLIPRG PSHPGLSAVHI*D*FLFLVKMIYYFC*ACLYLCV RSCWLAVRVHVESWCPEIGRPDAPSPALVQGSW PRVLAPEGHLPLPQPPPHTCSSSLK*SV*R*KC SSVINCVYSVNKE

Sequence BCL2L1.bAug05

CGCGCACTCCCTTTGCGTCTCGGGCTCGCGCGCGTTGCCGCGGCACCGGAAGTGACTGAGCTTGCAAGTTCCCCTGTCTCTTCAGGGGAAACTGAGGCCG GCTTGTTCGGGAGAGACGGCGCGAGCAGTCAGCCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGA GCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTT CCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCAT CAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCA GCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGA CAGCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTG CGTGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAG AACGGCGGCTGGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCTGACGGGCA TGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCTGCTCCACC ACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGTCCCTTGCA GCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACTTGTTCCAGCTCTTTGAAATAGTCTGTGTGAAGGTGAAAGTGCAGTTCAGTAATAA ACTGTGTTTACTCAGTGAACAAAGAGCC

BCLX-3 (forward primer)
BCLX-2 (reverse primer)
Single exon skipping

Frame 0

RALPLRLGLARVAAAPEVTELASSPVSSGETEA GLFGRDGASSQPESYLGFGS*KRITNQRRDSVSE QVFWTMDWLSPSLL*KCLRATGSWWLTFSPTSF PRKDTAGVSLVMWKRTGLRPQKGLNRRWRPPVP SMATHPGTWQTAPR*MEPLATAAVWMPGR*SPWQ Q*SKR*GRQATSLNCGTGGHSVT*HPSSTSPQG QHIRALNR**MNSSGMG*TGVALWPFSPSAGHC AWKA*TRRCRYW*VGSQLGWPLT*MTT*SLGSRR TAAGILLWNSMGTMQQPRAERARNASTAGS*RA *LWPAWFCWAHSSVGNDQTLTIHSTLPPPSLLH HILRPAAIATRRTTTCSPCPPAHHRVGPRSGPLQ LVF*NLSHFCETPTLVPAL*NSLCEGESAVQ** TVFTQ*TKS

Frame 1

AHSLCVSGSRALPRHRK*LSLQVPLSLQGKLRP ACSGETARAVSQNLILALDLRRESLTRDETQ*V SRCFGQWTG*AHPYYKNVSEQPGAGG*LSLLQAF PERIQLESV**CGREQD*GPRRD*IGDGDPQCH QWQPILAPGRQPRGEWSHWPQQQFGCPGGDPHG SSKASAEGGRRRV*TAVPAGIQ*PDIPAPHHPRD SISEL*TGSE*TLPGWGKLGSHCGLFLLRRGTV RGKRRQGDAGIGESDRSLDGHLPE*PPRALDPG ERRLGYFCGTLWEQCSSREPKGPGTLQPLVPDGH DCGRRGSAGLTLQSEMTRH*PSTLPSHPLLCST TSSVQPPLPPGEPLHAAHAHLPITGLGPDLVPC S*FSRIYHTSVRPPHLFQLFEIVCVKVKVQFSNK LCLLSEQRA

Frame 2

RTPFASRARARCRGTGSD*ACKFPCLFRGN*GR LVRERRREQSARILSWLWILEENH*PETRLSE* AGVLDNGLVEPIPIIKMSQSNRELVVDFLSYKL SQKGYSWSQFSDVEENRTEAPEGTESEMETPSAI NGNPSWHLADSPAVNGATGHSSSLDAREVIPMA AVKQALREAGDEFELRYRRAFSDLTSQLHITPG TAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALC VESVDKEMQVLVSRIAAWMATYLNDHLEPWIQE NGGWDTFVELYGNNAAAESRKGQERFNRWFLTG MTVAGVVLLGSLFSRK*PDTDHPLYPPTPFSAPP HPPSSRHCHQENHYMQPMPTCPSQGWAQIWSLA ASFLEFITLL*DPHTCSSSLK*SV*R*KCSSVI NCVYSVNKE

Sequence BCL2L1.aAug05

CGCGCACTCCCTTTGCGTCTCGGGCTCGCGCGCGTTGCCGCGGCACCGGAAGTGACTGAGCTTGCAAGTTCCCCTGTCTCTTCAGGGGAAACTGAGGCCG GCTTGTTCGGGAGAGACGGCGCGAGCAGTCAGCCAGGTAGGCCGGCAGCCAGGTAGGCCGGCCCGGGTCCGCGGCGCGGAACTCGGCCGCGAAGAGCTCT TGCGTCTGGAAGCTACCGGGCCGATGAAGGGGGATGTGGCCCCCCACGGCTCGCGGGGCTCGCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATC ACTAACCAGAGACGAGACTCAGTGAGTGAGCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCT GGTGGTTGACTTTCTCTCCTACAAGCTTTCCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACT GAATCGGAGATGGAGACCCCCAGTGCCATCAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTT TGGATGCCCGGGAGGTGATCCCCATGGCAGCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCT GACATCCCAGCTCCACATCACCCCAGGGACAGCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTG GCCTTTTTCTCCTTCGGCGGGGCACTGTGCGTGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGA ATGACCACCTAGAGCCTTGGATCCAGGAGAACGGCGGCTGGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGA ACGCTTCAACCGCTGGTTCCTGACGGGCATGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTC TACCCTCCCACCCCCTTCTCTGCTCCACCACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCAC AGGGTTGGGCCCAGATCTGGTCCCTTGCAGCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCAC AAAATTTCCACAAAATCTGTCCAAAGGAGGCTGGCAGGTATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCT TAGCCTCCCTGAAAATGTTTTTCTGCCAGGGAGCTTGAAAGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGC CTCAGAATTGATCATTTTCCCCCCACTCTCCCCACACTAACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGAC TAGGGATTCAGGCTGCTTGGGATAAAGATGCAAGGACCAGGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAG AAGCCTCTGGCTAGAGGCCAGCCCCACCCAGGAGGGAGGGGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACC ACCCTAGTCCCTTCCCCTCCCTGGCTCCCATGACCATACTGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCA CTTCCTACAAGAGCAGCCTGTGGCATCTTTGCCTTGGGCTGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAG CTATGGGAGCCCCAGGGTCTTCCCTACCTCAGGCAGGAAGGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTG CCTGGCCAGGCAGATCTGTGCCCCATGCCTGTCCAGCCTGGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCT CTTCTGCTCCACCCTTGGCCTGTCTCATCCCCAGGGGTCCCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGAT GATATACTATTTTTGTTAAGCGTGTCTGTATTTATGTGTGAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGG CCTGATGCTCCCTCCCCTGCCCTGGTCCAGGGAAGCTGGCCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTT CCAGCTCTTTGAAATAGTCTGTGTGAAGGTGAAAGTGCAGTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCLX-3 (forward primer)
BCLX-2 (reverse primer)
Single exon skipping

Frame 0

RALPLRLGLARVAAAPEVTELASSPVSSGETEA GLFGRDGASSQPGRPAAR*AGPGPRRGTRPRRAL ASGSYRADEGGCGPPRLAGLAESYLGFGS*KRI TNQRRDSVSEQVFWTMDWLSPSLL*KCLRATGS WWLTFSPTSFPRKDTAGVSLVMWKRTGLRPQKGL NRRWRPPVPSMATHPGTWQTAPR*MEPLATAAV WMPGR*SPWQQ*SKR*GRQATSLNCGTGGHSVT *HPSSTSPQGQHIRALNR**MNSSGMG*TGVALW PFSPSAGHCAWKA*TRRCRYW*VGSQLGWPLT* MTT*SLGSRRTAAGILLWNSMGTMQQPRAERAR NASTAGS*RA*LWPAWFCWAHSSVGNDQTLTIHS TLPPPSLLHHILRPAAIATRRTTTCSPCPPAHH RVGPRSGPLQLVF*NLSHFCETPTPQFPWPQNS QNFHKICPKEAGRYGRVCGWGQEGPT*LVQPLPL SLPENVFLPGSLKVFRTSSPERRLDCLCFDVCG LRIDHFPPTLPTLTWVPFPSIPTP*EPFRGHF* LGIQAAWDKDARTRTPSSPLDWLESSLPVQMSSR SLWLEASPTQEGGGYSYRKHPMPKLGWPLQFST TLVPSPPWLP*PY*GTNWAQDRCPRAVYGLSCL TSYKSSLWHLCLGLLLMVGSGDSALR*KGAIRNS YGSPRVFPTSGRKGRKESLLHGVG*G*LEGPVL PGQADLCPMPVQPGQPGCQGQSGLARSSSGLPL SSAPPLACLIPRGPSHPGLSAVHI*D*FLFLVKM IYYFC*ACLYLCVRSCWLAVRVHVESWCPEIGR PDAPSPALVQGSWPRVLAPEGHLPLPQPPPHTC SSSLK*SV*R*KCSSVINCVYSVNKE

Frame 1

AHSLCVSGSRALPRHRK*LSLQVPLSLQGKLRP ACSGETARAVSQVGRQPGRPARVRGAELGREEL LRLEATGPMKGDVAPHGSRGSQNLILALDLRRES LTRDETQ*VSRCFGQWTG*AHPYYKNVSEQPGA GG*LSLLQAFPERIQLESV**CGREQD*GPRRD *IGDGDPQCHQWQPILAPGRQPRGEWSHWPQQQF GCPGGDPHGSSKASAEGGRRRV*TAVPAGIQ*P DIPAPHHPRDSISEL*TGSE*TLPGWGKLGSHC GLFLLRRGTVRGKRRQGDAGIGESDRSLDGHLPE *PPRALDPGERRLGYFCGTLWEQCSSREPKGPG TLQPLVPDGHDCGRRGSAGLTLQSEMTRH*PST LPSHPLLCSTTSSVQPPLPPGEPLHAAHAHLPIT GLGPDLVPCS*FSRIYHTSVRPPHLSSLGLRIH KISTKSVQRRLAGMEGFVAGGRRALPDWCNPYP LASLKMFFCQGA*KFSEPLPQKGD*IAFVLMFVA SELIIFPPLSPH*PGFPFLPSLPPKSHLGATFD *GFRLLGIKMQGPGLPPHLWTG*SPHSQSKCPP EASG*RPAPPRREGAIATGSTPCQS*GGPCSSAP P*SLPLPGSHDHTEGPTGPKTDAPELFMASAAS LPTRAACGIFALGCSSWWVQGTQP*GERELSGT AMGAPGSSLPQAGRAGRRACCMGWGRAD*KGQSC LARQICAPCLSSLGSQAAKARVAWPGALQASLS LLLHPWPVSSPGVPATPGSLLYIFETSFYSL*R *YTIFVKRVCIYV*GAAGLQCACTWRAGARRLDG LMLPPLPWSREAGRGSWLLRGICPSPNPHPTLV PAL*NSLCEGESAVQ**TVFTQ*TKS

Frame 2

RTPFASRARARCRGTGSD*ACKFPCLFRGN*GR LVRERRREQSAR*AGSQVGRPGSAARNSAAKSS CVWKLPGR*RGMWPPTARGARRILSWLWILEEN H*PETRLSE*AGVLDNGLVEPIPIIKMSQSNREL VVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGT ESEMETPSAINGNPSWHLADSPAVNGATGHSSS LDAREVIPMAAVKQALREAGDEFELRYRRAFSDL TSQLHITPGTAYQSFEQVVNELFRDGVNWGRIV AFFSFGGALCVESVDKEMQVLVSRIAAWMATYL NDHLEPWIQENGGWDTFVELYGNNAAAESRKGQE RFNRWFLTGMTVAGVVLLGSLFSRK*PDTDHPL YPPTPFSAPPHPPSSRHCHQENHYMQPMPTCPS QGWAQIWSLAASFLEFITLL*DPHTSVPLASEFT KFPQNLSKGGWQVWKGLWLGAGGPYLIGATLTP *PP*KCFSARELESFQNLFPRKETRLPLF*CLW PQN*SFSPHSPHTNLGSLSFHPYPLRAI*GPLLT RDSGCLG*RCKDQDSLLTSGLARVLTPSPNVLQ KPLARGQPHPGGRGL*LQEAPHAKARVALAVQH HPSPFPSLAPMTILRDQLGPRQMPQSCLWPQLPH FLQEQPVASLPWAAPHGGFRGLSPEVKGSYQEQ LWEPQGLPYLRQEGQEGEPAAWGGVGLTRRASP AWPGRSVPHACPAWAARLPRPEWPGQELFRPPSL FCSTLGLSHPQGSQPPRALCCTYLRLVFIPCED DILFLLSVSVFMCEELLACSARARGELVPGDWT A*CSLPCPGPGKLAEGPGS*GASAPPPTPTPHLF QLFEIVCVKVKVQFSNKLCLLSEQRA

Sequence BCL2L1.eAug05

CGCGCACTCCCTTTGCGTCTCGGGCTCGCGCGCGTTGCCGCGGCACCGGAAGTGACTGAGCTTGCAAGTTCCCCTGTCTCTTCAGGGGAAACTGAGGCCG GCTTGTTCGGGAGAGACGGCGCGAGCAGTCAGCCAGGTAGGCCGGCAGCCAGGTAGGCCGGCCCGGGTCCGCGGCGCGGAACTCGGCCGCGAAGAGCTCT TGCGTCTGGAAGCTACCGGGCCGATGAAGGGGGATGTGGCCCCCCACGGCTCGCGGGGCTCGCAGCTCTTTCTCTCCCTTCAGAATCTTATCTTGGCTTT GGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGAGCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTC TCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTTCCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACT GAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCATCAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCA CTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCAGCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCG GCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGACAGCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTA AACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTGCGTGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTT GGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAGAACGGCGGCTGGGTGCCAGATGCCAGTTTTAGATTCTGGCTCCACCACTGACA CAGCTGGGTGACCTTGATCCAGTCCCTGGACCCTCAGAGGCACAGCTTCTTGGCCTGTAAATGGGGAGAGACAGCCTGCATTGCAGAATTGTTGGGAGGA TTAAAGGACAAATTGGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCTGACG GGCATGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCTGCTC CACCACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGTCCCT TGCAGCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCACAAAATCTGTCCAAA GGAGGCTGGCAGGTATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCTGAAAATGTTTTTCTG CCAGGGAGCTTGAAAGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTGATCATTTTCCCCCCA CTCTCCCCACACTAACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCAGGCTGCTTGGGATAA AGATGCAAGGACCAGGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGGCTAGAGGCCAGCCCC ACCCAGGAGGGAGGGGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCCCTTCCCCTCCCTGGC TCCCATGACCATACTGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAAGAGCAGCCTGTGGCA TCTTTGCCTTGGGCTGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGCCCCAGGGTCTTCCCT ACCTCAGGCAGGAAGGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGGCAGATCTGTGCCCCA TGCCTGTCCAGCCTGGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCCACCCTTGGCCTGTCT CATCCCCAGGGGTCCCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTATTTTTGTTAAGCGTGT CTGTATTTATGTGTGAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTCCCTCCCCTGCCCTGG TCCAGGGAAGCTGGCCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTTGAAATAGTCTGTGTG AAGGTGAAAGTGCAGTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCLX-3 (forward primer)
BCLX-2 (reverse primer)
Single exon skipping

Frame 0

RALPLRLGLARVAAAPEVTELASSPVSSGETEA GLFGRDGASSQPGRPAAR*AGPGPRRGTRPRRAL ASGSYRADEGGCGPPRLAGLAALSLPSESYLGF GS*KRITNQRRDSVSEQVFWTMDWLSPSLL*KC LRATGSWWLTFSPTSFPRKDTAGVSLVMWKRTGL RPQKGLNRRWRPPVPSMATHPGTWQTAPR*MEP LATAAVWMPGR*SPWQQ*SKR*GRQATSLNCGT GGHSVT*HPSSTSPQGQHIRALNR**MNSSGMG* TGVALWPFSPSAGHCAWKA*TRRCRYW*VGSQL GWPLT*MTT*SLGSRRTAAGCQMPVLDSGSTTD TAG*P*SSPWTLRGTASWPVNGERQPALQNCWED *RTNWDTFVELYGNNAAAESRKGQERFNRWFLT GMTVAGVVLLGSLFSRK*PDTDHPLYPPTPFSA PPHPPSSRHCHQENHYMQPMPTCPSQGWAQIWSL AASFLEFITLL*DPHTSVPLASEFTKFPQNLSK GGWQVWKGLWLGAGGPYLIGATLTP*PP*KCFS ARELESFQNLFPRKETRLPLF*CLWPQN*SFSPH SPHTNLGSLSFHPYPLRAI*GPLLTRDSGCLG* RCKDQDSLLTSGLARVLTPSPNVLQKPLARGQP HPGGRGL*LQEAPHAKARVALAVQHHPSPFPSLA PMTILRDQLGPRQMPQSCLWPQLPHFLQEQPVA SLPWAAPHGGFRGLSPEVKGSYQEQLWEPQGLP YLRQEGQEGEPAAWGGVGLTRRASPAWPGRSVPH ACPAWAARLPRPEWPGQELFRPPSLFCSTLGLS HPQGSQPPRALCCTYLRLVFIPCEDDILFLLSV SVFMCEELLACSARARGELVPGDWTA*CSLPCPG PGKLAEGPGS*GASAPPPTPTPHLFQLFEIVCV KVKVQFSNKLCLLSEQRA

Frame 1

AHSLCVSGSRALPRHRK*LSLQVPLSLQGKLRP ACSGETARAVSQVGRQPGRPARVRGAELGREEL LRLEATGPMKGDVAPHGSRGSQLFLSLQNLILAL DLRRESLTRDETQ*VSRCFGQWTG*AHPYYKNV SEQPGAGG*LSLLQAFPERIQLESV**CGREQD *GPRRD*IGDGDPQCHQWQPILAPGRQPRGEWSH WPQQQFGCPGGDPHGSSKASAEGGRRRV*TAVP AGIQ*PDIPAPHHPRDSISEL*TGSE*TLPGWG KLGSHCGLFLLRRGTVRGKRRQGDAGIGESDRSL DGHLPE*PPRALDPGERRLGARCQF*ILAPPLT QLGDLDPVPGPSEAQLLGL*MGRDSLHCRIVGR IKGQIGILLWNSMGTMQQPRAERARNASTAGS*R A*LWPAWFCWAHSSVGNDQTLTIHSTLPPPSLL HHILRPAAIATRRTTTCSPCPPAHHRVGPRSGP LQLVF*NLSHFCETPTPQFPWPQNSQNFHKICPK EAGRYGRVCGWGQEGPT*LVQPLPLSLPENVFL PGSLKVFRTSSPERRLDCLCFDVCGLRIDHFPP TLPTLTWVPFPSIPTP*EPFRGHF*LGIQAAWDK DARTRTPSSPLDWLESSLPVQMSSRSLWLEASP TQEGGGYSYRKHPMPKLGWPLQFSTTLVPSPPW LP*PY*GTNWAQDRCPRAVYGLSCLTSYKSSLWH LCLGLLLMVGSGDSALR*KGAIRNSYGSPRVFP TSGRKGRKESLLHGVG*G*LEGPVLPGQADLCP MPVQPGQPGCQGQSGLARSSSGLPLSSAPPLACL IPRGPSHPGLSAVHI*D*FLFLVKMIYYFC*AC LYLCVRSCWLAVRVHVESWCPEIGRPDAPSPAL VQGSWPRVLAPEGHLPLPQPPPHTCSSSLK*SV* R*KCSSVINCVYSVNKE

Frame 2

RTPFASRARARCRGTGSD*ACKFPCLFRGN*GR LVRERRREQSAR*AGSQVGRPGSAARNSAAKSS CVWKLPGR*RGMWPPTARGARSSFSPFRILSWL WILEENH*PETRLSE*AGVLDNGLVEPIPIIKMS QSNRELVVDFLSYKLSQKGYSWSQFSDVEENRT EAPEGTESEMETPSAINGNPSWHLADSPAVNGA TGHSSSLDAREVIPMAAVKQALREAGDEFELRYR RAFSDLTSQLHITPGTAYQSFEQVVNELFRDGV NWGRIVAFFSFGGALCVESVDKEMQVLVSRIAA WMATYLNDHLEPWIQENGGWVPDASFRFWLHH*H SWVTLIQSLDPQRHSFLACKWGETACIAELLGG LKDKLGYFCGTLWEQCSSREPKGPGTLQPLVPD GHDCGRRGSAGLTLQSEMTRH*PSTLPSHPLLCS TTSSVQPPLPPGEPLHAAHAHLPITGLGPDLVP CS*FSRIYHTSVRPPHLSSLGLRIHKISTKSVQ RRLAGMEGFVAGGRRALPDWCNPYPLASLKMFFC QGA*KFSEPLPQKGD*IAFVLMFVASELIIFPP LSPH*PGFPFLPSLPPKSHLGATFD*GFRLLGI KMQGPGLPPHLWTG*SPHSQSKCPPEASG*RPAP PRREGAIATGSTPCQS*GGPCSSAPP*SLPLPG SHDHTEGPTGPKTDAPELFMASAASLPTRAACG IFALGCSSWWVQGTQP*GERELSGTAMGAPGSSL PQAGRAGRRACCMGWGRAD*KGQSCLARQICAP CLSSLGSQAAKARVAWPGALQASLSLLLHPWPV SSPGVPATPGSLLYIFETSFYSL*R*YTIFVKRV CIYV*GAAGLQCACTWRAGARRLDGLMLPPLPW SREAGRGSWLLRGICPSPNPHPTLVPAL*NSLC EGESAVQ**TVFTQ*TKS