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Sequence BIRC5.dAug05
CGCCTCTACTCCCAGAAGGCCGCGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGCGCGCCATTAACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGGTGGCGGCGGCGGCATGGGTGCCCCGACGTTGCCCCCTGCCTGGCAGCCCTTTCTCAAGGACCACCGCATCTCTACATTCAAG
AACTGGCCCTTCTTGGAGGGCTGCGCCTGCACCCCGGAGCGGATGGCCGAGGCTGGCTTCATCCACTGCCCCACTGAGAACGAGCCAGACTTGGCCCAGT
GTTTCTTCTGCTTCAAGGAGCTGGAAGGCTGGGAGCCAGATGACGACCCCATGCAAAGGAAACCAACAATAAGAAGAAAGAATTTGAGGAAACTGCGGAG
AAAGTGCGCCGTGCCATCGAGCAGCTGGCTGCCATGGATTGAGGCCTCTGGCCGGAGCTGCCTGGTCCCAGAGTGGCTGCACCACTTCCAGGGTTTATTC
CCTGGTGCCACCAGCCTTCCTGTGGGCCCCTTAGCAATGTCTTAGGAAAGGAGATCAACATTTTCAAATTAGATGTTTCAACTGTGCTCTTGTTTTGTCT
TGAAAGTGGCACCAGAGGTGCTTCTGCCTGTGCAGCGGGTGCTGCTGGTAACAGTGGCTGCTTCTCTCTCTCTCTCTCTTTTTTGGGGGCTCATTTTTGC
TGTTTTGATTCCCGGGCTTACCAGGTGAGAAGTGAGGGAGGAAGAAGGCAGTGTCCCTTTTGCTAGAGCTGACAGCTTTGTTCGCGTGGGCAGAGCCTTC
CACAGTGAATGTGTCTGGACCTCATGTTGTTGAGGCTGTCACAGTCCTGAGTGTGGACTTGGCAGGTGCCTGTTGAATCTGAGCTGCAGGTTCCTTATCT
GTCACACCTGTGCCTCCTCAGAGGACAGTTTTTTTGTTGTTGTGTTTTTTTGTTTTTTTTTTTTTGGTAGATGCATGACTTGTGTGTGATGAGAGAATGG
AGACAGAGTCCCTGGCTCCTCTACTGTTTAACAACATGGCTTTCTTATTTTGTTTGAATTGTTAATTCACAGAATAGCACAAACTACAATTAAAACTAAG
CACAAAGCCATTCTAAGTCATTGGGGAAACGGGGTGAACTTCAGGTGGATGAGGAGACAGAATAGAGTGATAGGAAGCGTCTGGCAGATACTCCTTTTGC
CACTGCTGTGTGATTAGACAGGCCCAGTGAGCCGCGGGGCACATGCTGGCCGCTCCTCCCTCAGAAAAAGGCAGTGGCCTAAATCCTTTTTAAATGACTT
GGCTCGATGCTGTGGGGGACTGGCTGGGCTGCTGCAGGCCGTGTGTCTGTCAGCCCAACCTTCACATCTGTCACGTTCTCCACACGGGGGAGAGACGCAG
TCCGCCCAGGTCCCCGCTTTCTTTGGAGGCAGCAGCTCCCGCAGGGCTGAAGTCTGGCGTAAGATGATGGATTTGATTCGCCCTCCTCCCTGTCATAGAG
CTGCAGGGTGGATTGTTACAGCTTCGCTGGAAACCTCTGGAGGTCATCTCGGCTGTTCCTGAGAAATAAAAAGCCTGTCATTTCAAACACTGCTGTGGAC
CCTACTGGGTTTTTAAAATATTGTCAGTTTTTCATCGTCGTCCCTAGCCTGCCAACAGCCATCTGCCCAGACAGCCGCAGTGAGGATGAGCGTCCTGGCA
GAGACGCAGTTGTCTCTGGGCGCTTGCCAGAGCCACGAACCCCAGACCTGTTTGTATCATCCGGGCTCCTTCCGGGCAGAAACAACTGAAAATGCACTTC
AGACCCACTTATTTCTGCCACATCTGAGTCGGCCTGAGATAGACTTTTCCCTCTAAACTGGGAGAATATCACAGTGGTTTTTGTTAGCAGAAAATGCACT
CCAGCCTCTGTACTCATCTAAGCTGCTTATTTTTGATATTTGTGTCAGTCTGTAAATGGATACTTCACTTTAATAACTGTTGCTTAGTAATTGGCTTTGT
AGAGAAGCTGGAAAAAAATGGTTTTGTCTTCAACTCCTTTGCATGCCAGGCGGTGATGTGGATCTCGGCTTCTGTGAGCCTGTGCTGTGGGCAGGGCTGA
GCTGGAGCCGCCCCTCTCAGCCCGCCTGCCACGGCCTTTCCTTAAAGGCCATCCTTAAAACCAGACCCTCATGGCTACCAGCACCTGAAAGCTTCCTCGA
CATCTGTTAATAAAGCCGTAGGCCCTTGTCTAAGTGCAACCGCCTAGACTTTCTTTCAGATACATGTCCACATGTCCATTTTTCAGGTTCTCTAAGTTGG
AGTGGAGTCTGGGAAGGGTTGTGAATGAGGCTTCTGGGCTATGGGTGAGGTTCCAATGGCAGGTTAGAGCCCCTCGGGCCAACTGCCATCCTGGAAAGTA
GAGACAGCAGTGCCCGCTGCCCAGAAGAGACCAGCAAGCCAAACTGGAGCCCCCATTGCAGGCTGTCGCCATGTGGAAAGAGTAACTCACAATTGCCAAT
AAAGTCTCATGTGGTTTTATCT
- primer-1293 (forward primer)
- primer-1294 (reverse primer)
- Multiple exon skipping
Frame 0
RLYSQKAAGGGPPKRACAPDMPRGAPLTARFESRDPLAEVAAAAWVPRRCPLPGSPFSRTTASLHSR
TGPSWRAAPAPRSGWPRLASSTAPLRTSQTWPS
VSSASRSWKAGSQMTTPCKGNQQ*EERI*GNCG
ESAPCHRAAGCHGLRPLAGAAWSQSGCTTSRVYS
LVPPAFLWAP*QCLRKGDQHFQIRCFNCALVLS
*KWHQRCFCLCSGCCW*QWLLLSLSLFFGGSFL
LF*FPGLPGEK*GRKKAVSLLLELTALFAWAEPS
TVNVSGPHVVEAVTVLSVDLAGAC*I*AAGSLS
VTPVPPQRTVFLLLCFFVFFFLVDA*LVCDERM
ETESLAPLLFNNMAFLFCLNC*FTE*HKLQLKLS
TKPF*VIGETG*TSGG*GDRIE**EASGRYSFC
HCCVIRQAQ*AAGHMLAAPPSEKGSGLNPF*MT
WLDAVGDWLGCCRPCVCQPNLHICHVLHTGERRS
PPRSPLSLEAAAPAGLKSGVR*WI*FALLPVIE
LQGGLLQLRWKPLEVISAVPEK*KACHFKHCCG
PYWVFKILSVFHRRP*PANSHLPRQPQ*G*ASWQ
RRSCLWALARATNPRPVCIIRAPSGQKQLKMHF
RPTYFCHI*VGLR*TFPSKLGEYHSGFC*QKMH
SSLCTHLSCLFLIFVSVCKWILHFNNCCLVIGFV
EKLEKNGFVFNSFACQAVMWISASVSLCCGQG*
AGAAPLSPPATAFP*RPSLKPDPHGYQHLKASS
TSVNKAVGPCLSATA*TFFQIHVHMSIFQVL*VG
VESGKGCE*GFWAMGEVPMAG*SPSGQLPSWKV
ETAVPAAQKRPASQTGAPIAGCRHVERVTHNCQ
*SLMWFY
Frame 1
ASTPRRPRGVDRLRGRALPTCPAARH*PPDLNRGTRWQRWRRRHGCPDVAPCLAALSQGPPHLYIQ
ELALLGGLRLHPGADGRGWLHPLPH*ERARLGPV
FLLLQGAGRLGAR*RPHAKETNNKKKEFEETAE
KVRRAIEQLAAMD*GLWPELPGPRVAAPLPGFI
PWCHQPSCGPLSNVLGKEINIFKLDVSTVLLFCL
ESGTRGASACAAGAAGNSGCFSLSLSFLGAHFC
CFDSRAYQVRSEGGRRQCPFC*S*QLCSRGQSL
PQ*MCLDLMLLRLSQS*VWTWQVPVESELQVPYL
SHLCLLRGQFFCCCVFLFFFFW*MHDLCVMREW
RQSPWLLYCLTTWLSYFV*IVNSQNSTNYN*N*
AQSHSKSLGKRGELQVDEETE*SDRKRLADTPFA
TAV*LDRPSEPRGTCWPLLPQKKAVA*ILFK*L
GSMLWGTGWAAAGRVSVSPTFTSVTFSTRGRDA
VRPGPRFLWRQQLPQG*SLA*DDGFDSPSSLS*S
CRVDCYSFAGNLWRSSRLFLRNKKPVISNTAVD
PTGFLKYCQFFIVVPSLPTAICPDSRSEDERPG
RDAVVSGRLPEPRTPDLFVSSGLLPGRNN*KCTS
DPLISATSESA*DRLFPLNWENITVVFVSRKCT
PASVLI*AAYF*YLCQSVNGYFTLITVA**LAL
*RSWKKMVLSSTPLHARR*CGSRLL*ACAVGRAE
LEPPLSARLPRPFLKGHP*NQTLMATST*KLPR
HLLIKP*ALV*VQPPRLSFRYMSTCPFFRFSKL
EWSLGRVVNEASGLWVRFQWQVRAPRANCHPGK*
RQQCPLPRRDQQAKLEPPLQAVAMWKE*LTIAN
KVSCGFI
Frame 2
PLLPEGRGGWTA*EGVRSRHAPRRAINRQI*IAGPVGRGGGGGMGAPTLPPAWQPFLKDHRISTFK
NWPFLEGCACTPERMAEAGFIHCPTENEPDLAQ
CFFCFKELEGWEPDDDPMQRKPTIRRKNLRKLRR
KCAVPSSSWLPWIEASGRSCLVPEWLHHFQGLF
PGATSLPVGPLAMS*ERRSTFSN*MFQLCSCFV
LKVAPEVLLPVQRVLLVTVAASLSLSLFWGLIFA
VLIPGLTR*EVREEEGSVPFARADSFVRVGRAF
HSECVWTSCC*GCHSPECGLGRCLLNLSCRFLI
CHTCASSEDSFFVVVFFCFFFFGRCMTCV**ENG
DRVPGSSTV*QHGFLILFELLIHRIAQTTIKTK
HKAILSHWGNGVNFRWMRRQNRVIGSVWQILLL
PLLCD*TGPVSRGAHAGRSSLRKRQWPKSFLNDL
ARCCGGLAGLLQAVCLSAQPSHLSRSPHGGETQ
SAQVPAFFGGSSSRRAEVWRKMMDLIRPPPCHR
AAGWIVTASLETSGGHLGCS*EIKSLSFQTLLWT
LLGF*NIVSFSSSSLACQQPSAQTAAVRMSVLA
ETQLSLGACQSHEPQTCLYHPGSFRAETTENAL
QTHLFLPHLSRPEIDFSL*TGRISQWFLLAENAL
QPLYSSKLLIFDICVSL*MDTSL**LLLSNWLC
REAGKKWFCLQLLCMPGGDVDLGFCEPVLWAGL
SWSRPSQPACHGLSLKAILKTRPSWLPAPESFLD
IC**SRRPLSKCNRLDFLSDTCPHVHFSGSLSW
SGVWEGL*MRLLGYG*GSNGRLEPLGPTAILES
RDSSARCPEETSKPNWSPHCRLSPCGKSNSQLPI
KSHVVLS
Sequence BIRC5.aAug05
CGCCTCTACTCCCAGAAGGCCGCGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGCGCGCCATTAACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGGTGGCGGCGGCGGCATGGGTGCCCCGACGTTGCCCCCTGCCTGGCAGCCCTTTCTCAAGGACCACCGCATCTCTACATTCAAG
AACTGGCCCTTCTTGGAGGGCTGCGCCTGCACCCCGGAGCGGATGGCCGAGGCTGGCTTCATCCACTGCCCCACTGAGAACGAGCCAGACTTGGCCCAGT
GTTTCTTCTGCTTCAAGGAGCTGGAAGGCTGGGAGCCAGATGACGACCCCATTGGGCCGGGCACGGTGGCTTACGCCTGTAATACCAGCACTTTGGGAGG
CCGAGGCGGGCGGATCACGAGAGAGGAACATAAAAAGCATTCGTCCGGTTGCGCTTTCCTTTCTGTCAAGAAGCAGTTTGAAGAATTAACCCTTGGTGAA
TTTTTGAAACTGGACAGAGAAAGAGCCAAGAACAAAATTGCAAAGGAAACCAACAATAAGAAGAAAGAATTTGAGGAAACTGCGGAGAAAGTGCGCCGTG
CCATCGAGCAGCTGGCTGCCATGGATTGAGGCCTCTGGCCGGAGCTGCCTGGTCCCAGAGTGGCTGCACCACTTCCAGGGTTTATTCCCTGGTGCCACCA
GCCTTCCTGTGGGCCCCTTAGCAATGTCTTAGGAAAGGAGATCAACATTTTCAAATTAGATGTTTCAACTGTGCTCTTGTTTTGTCTTGAAAGTGGCACC
AGAGGTGCTTCTGCCTGTGCAGCGGGTGCTGCTGGTAACAGTGGCTGCTTCTCTCTCTCTCTCTCTTTTTTGGGGGCTCATTTTTGCTGTTTTGATTCCC
GGGCTTACCAGGTGAGAAGTGAGGGAGGAAGAAGGCAGTGTCCCTTTTGCTAGAGCTGACAGCTTTGTTCGCGTGGGCAGAGCCTTCCACAGTGAATGTG
TCTGGACCTCATGTTGTTGAGGCTGTCACAGTCCTGAGTGTGGACTTGGCAGGTGCCTGTTGAATCTGAGCTGCAGGTTCCTTATCTGTCACACCTGTGC
CTCCTCAGAGGACAGTTTTTTTGTTGTTGTGTTTTTTTGTTTTTTTTTTTTTGGTAGATGCATGACTTGTGTGTGATGAGAGAATGGAGACAGAGTCCCT
GGCTCCTCTACTGTTTAACAACATGGCTTTCTTATTTTGTTTGAATTGTTAATTCACAGAATAGCACAAACTACAATTAAAACTAAGCACAAAGCCATTC
TAAGTCATTGGGGAAACGGGGTGAACTTCAGGTGGATGAGGAGACAGAATAGAGTGATAGGAAGCGTCTGGCAGATACTCCTTTTGCCACTGCTGTGTGA
TTAGACAGGCCCAGTGAGCCGCGGGGCACATGCTGGCCGCTCCTCCCTCAGAAAAAGGCAGTGGCCTAAATCCTTTTTAAATGACTTGGCTCGATGCTGT
GGGGGACTGGCTGGGCTGCTGCAGGCCGTGTGTCTGTCAGCCCAACCTTCACATCTGTCACGTTCTCCACACGGGGGAGAGACGCAGTCCGCCCAGGTCC
CCGCTTTCTTTGGAGGCAGCAGCTCCCGCAGGGCTGAAGTCTGGCGTAAGATGATGGATTTGATTCGCCCTCCTCCCTGTCATAGAGCTGCAGGGTGGAT
TGTTACAGCTTCGCTGGAAACCTCTGGAGGTCATCTCGGCTGTTCCTGAGAAATAAAAAGCCTGTCATTTCAAACACTGCTGTGGACCCTACTGGGTTTT
TAAAATATTGTCAGTTTTTCATCGTCGTCCCTAGCCTGCCAACAGCCATCTGCCCAGACAGCCGCAGTGAGGATGAGCGTCCTGGCAGAGACGCAGTTGT
CTCTGGGCGCTTGCCAGAGCCACGAACCCCAGACCTGTTTGTATCATCCGGGCTCCTTCCGGGCAGAAACAACTGAAAATGCACTTCAGACCCACTTATT
TCTGCCACATCTGAGTCGGCCTGAGATAGACTTTTCCCTCTAAACTGGGAGAATATCACAGTGGTTTTTGTTAGCAGAAAATGCACTCCAGCCTCTGTAC
TCATCTAAGCTGCTTATTTTTGATATTTGTGTCAGTCTGTAAATGGATACTTCACTTTAATAACTGTTGCTTAGTAATTGGCTTTGTAGAGAAGCTGGAA
AAAAATGGTTTTGTCTTCAACTCCTTTGCATGCCAGGCGGTGATGTGGATCTCGGCTTCTGTGAGCCTGTGCTGTGGGCAGGGCTGAGCTGGAGCCGCCC
CTCTCAGCCCGCCTGCCACGGCCTTTCCTTAAAGGCCATCCTTAAAACCAGACCCTCATGGCTACCAGCACCTGAAAGCTTCCTCGACATCTGTTAATAA
AGCCGTAGGCCCTTGTCTAAGTGCAACCGCCTAGACTTTCTTTCAGATACATGTCCACATGTCCATTTTTCAGGTTCTCTAAGTTGGAGTGGAGTCTGGG
AAGGGTTGTGAATGAGGCTTCTGGGCTATGGGTGAGGTTCCAATGGCAGGTTAGAGCCCCTCGGGCCAACTGCCATCCTGGAAAGTAGAGACAGCAGTGC
CCGCTGCCCAGAAGAGACCAGCAAGCCAAACTGGAGCCCCCATTGCAGGCTGTCGCCATGTGGAAAGAGTAACTCACAATTGCCAATAAAGTCTCATGTG
GTTTTATCT
- primer-1293 (forward primer)
- primer-1294 (reverse primer)
- Multiple exon skipping
Frame 0
RLYSQKAAGGGPPKRACAPDMPRGAPLTARFESRDPLAEVAAAAWVPRRCPLPGSPFSRTTASLHSR
TGPSWRAAPAPRSGWPRLASSTAPLRTSQTWPS
VSSASRSWKAGSQMTTPLGRARWLTPVIPALWE
AEAGGSRERNIKSIRPVALSFLSRSSLKN*PLVN
F*NWTEKEPRTKLQRKPTIRRKNLRKLRRKCAV
PSSSWLPWIEASGRSCLVPEWLHHFQGLFPGAT
SLPVGPLAMS*ERRSTFSN*MFQLCSCFVLKVAP
EVLLPVQRVLLVTVAASLSLSLFWGLIFAVLIP
GLTR*EVREEEGSVPFARADSFVRVGRAFHSEC
VWTSCC*GCHSPECGLGRCLLNLSCRFLICHTCA
SSEDSFFVVVFFCFFFFGRCMTCV**ENGDRVP
GSSTV*QHGFLILFELLIHRIAQTTIKTKHKAI
LSHWGNGVNFRWMRRQNRVIGSVWQILLLPLLCD
*TGPVSRGAHAGRSSLRKRQWPKSFLNDLARCC
GGLAGLLQAVCLSAQPSHLSRSPHGGETQSAQV
PAFFGGSSSRRAEVWRKMMDLIRPPPCHRAAGWI
VTASLETSGGHLGCS*EIKSLSFQTLLWTLLGF
*NIVSFSSSSLACQQPSAQTAAVRMSVLAETQL
SLGACQSHEPQTCLYHPGSFRAETTENALQTHLF
LPHLSRPEIDFSL*TGRISQWFLLAENALQPLY
SSKLLIFDICVSL*MDTSL**LLLSNWLCREAG
KKWFCLQLLCMPGGDVDLGFCEPVLWAGLSWSRP
SQPACHGLSLKAILKTRPSWLPAPESFLDIC**
SRRPLSKCNRLDFLSDTCPHVHFSGSLSWSGVW
EGL*MRLLGYG*GSNGRLEPLGPTAILESRDSSA
RCPEETSKPNWSPHCRLSPCGKSNSQLPIKSHV
VLS
Frame 1
ASTPRRPRGVDRLRGRALPTCPAARH*PPDLNRGTRWQRWRRRHGCPDVAPCLAALSQGPPHLYIQ
ELALLGGLRLHPGADGRGWLHPLPH*ERARLGPV
FLLLQGAGRLGAR*RPHWAGHGGLRL*YQHFGR
PRRADHERGT*KAFVRLRFPFCQEAV*RINPW*
IFETGQRKSQEQNCKGNQQ*EERI*GNCGESAPC
HRAAGCHGLRPLAGAAWSQSGCTTSRVYSLVPP
AFLWAP*QCLRKGDQHFQIRCFNCALVLS*KWH
QRCFCLCSGCCW*QWLLLSLSLFFGGSFLLF*FP
GLPGEK*GRKKAVSLLLELTALFAWAEPSTVNV
SGPHVVEAVTVLSVDLAGAC*I*AAGSLSVTPV
PPQRTVFLLLCFFVFFFLVDA*LVCDERMETESL
APLLFNNMAFLFCLNC*FTE*HKLQLKLSTKPF
*VIGETG*TSGG*GDRIE**EASGRYSFCHCCV
IRQAQ*AAGHMLAAPPSEKGSGLNPF*MTWLDAV
GDWLGCCRPCVCQPNLHICHVLHTGERRSPPRS
PLSLEAAAPAGLKSGVR*WI*FALLPVIELQGG
LLQLRWKPLEVISAVPEK*KACHFKHCCGPYWVF
KILSVFHRRP*PANSHLPRQPQ*G*ASWQRRSC
LWALARATNPRPVCIIRAPSGQKQLKMHFRPTY
FCHI*VGLR*TFPSKLGEYHSGFC*QKMHSSLCT
HLSCLFLIFVSVCKWILHFNNCCLVIGFVEKLE
KNGFVFNSFACQAVMWISASVSLCCGQG*AGAA
PLSPPATAFP*RPSLKPDPHGYQHLKASSTSVNK
AVGPCLSATA*TFFQIHVHMSIFQVL*VGVESG
KGCE*GFWAMGEVPMAG*SPSGQLPSWKVETAV
PAAQKRPASQTGAPIAGCRHVERVTHNCQ*SLMW
FY
Frame 2
PLLPEGRGGWTA*EGVRSRHAPRRAINRQI*IAGPVGRGGGGGMGAPTLPPAWQPFLKDHRISTFK
NWPFLEGCACTPERMAEAGFIHCPTENEPDLAQ
CFFCFKELEGWEPDDDPIGPGTVAYACNTSTLGG
RGGRITREEHKKHSSGCAFLSVKKQFEELTLGE
FLKLDRERAKNKIAKETNNKKKEFEETAEKVRR
AIEQLAAMD*GLWPELPGPRVAAPLPGFIPWCHQ
PSCGPLSNVLGKEINIFKLDVSTVLLFCLESGT
RGASACAAGAAGNSGCFSLSLSFLGAHFCCFDS
RAYQVRSEGGRRQCPFC*S*QLCSRGQSLPQ*MC
LDLMLLRLSQS*VWTWQVPVESELQVPYLSHLC
LLRGQFFCCCVFLFFFFW*MHDLCVMREWRQSP
WLLYCLTTWLSYFV*IVNSQNSTNYN*N*AQSHS
KSLGKRGELQVDEETE*SDRKRLADTPFATAV*
LDRPSEPRGTCWPLLPQKKAVA*ILFK*LGSML
WGTGWAAAGRVSVSPTFTSVTFSTRGRDAVRPGP
RFLWRQQLPQG*SLA*DDGFDSPSSLS*SCRVD
CYSFAGNLWRSSRLFLRNKKPVISNTAVDPTGF
LKYCQFFIVVPSLPTAICPDSRSEDERPGRDAVV
SGRLPEPRTPDLFVSSGLLPGRNN*KCTSDPLI
SATSESA*DRLFPLNWENITVVFVSRKCTPASV
LI*AAYF*YLCQSVNGYFTLITVA**LAL*RSWK
KMVLSSTPLHARR*CGSRLL*ACAVGRAELEPP
LSARLPRPFLKGHP*NQTLMATST*KLPRHLLI
KP*ALV*VQPPRLSFRYMSTCPFFRFSKLEWSLG
RVVNEASGLWVRFQWQVRAPRANCHPGK*RQQC
PLPRRDQQAKLEPPLQAVAMWKE*LTIANKVSC
GFI
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