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Sequence BID.hAug05
GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCCCGCGGCTGGAGGGTGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCC
GGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAG
GCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATG
TCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGT
GACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAG
ATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATT
TGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAAT
TGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGC
AGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTA
AGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGG
GGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACT
GTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTAC
TGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGA
ACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAG
GTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAG
CACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGC
ACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGC
AATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAA
ATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACA
CATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAA
AGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTA
GGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCA
ACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTT
GCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGA
AATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATT
CCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTT
TATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACA
AAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTC
TCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGG
AAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCT
GCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAG
GAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTT
TGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTAC
TTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGG
TGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTT
AACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAA
ATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTG
GCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTAT
AATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG
- BID.u.f.10 (forward primer)
- BID.u.r.14 (reverse primer)
- Single exon skipping
Frame 0
EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAPPRLEGDSESQEDIIRNIARHLAQVGDSMDRSIPP
GLVNGLALQLRNTSRSEEDRNRDLATALEQLLQ
AYPRDMEKEKTMLVLALLLAKKVASHTPSLLRD
VFHTTVNFINQNLRTYVRSLARNGMD*TDSSRSV
TG*SSMWSQLYSCFQCRRSPGMSTAFLEKTGWK
IAVTSILKTMLNL*PTLKYLH*YT*MKMSIYTY
LNGLHIIHT*ICTSVNLHTVPLFPLCRFPLKN*I
GKQVSRK*KQNTIFLVKKNYCLLKYNHRGWSYS
RQYPV*GKQESEKEHTPYK*KIPLKIGTIYLNT
KEPTIFLFKKPHGTEILN*SAALK*WEVRPQYTG
A*LFQLRFLCWSQKEPLVVLKGVKVI*GPRSAT
VCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQKL
LRCPGS*PPGNSCKLPISSHTLSSSMPQKCIWRE
QVSKHKR*KSSWTF*KFSKVVPSLRDSQNQVT*
VVPK*IRKSLALETVQHWSAVPVVSYVPGISLK
ARQWMLGPYHTHCCEQETPVTTT*GHWRRI*VRA
LTDS*FLLTRCFPVL*EFKIIRKEKQTLYCSAC
NTYHARAGSIESGQKLTNTKKKCCRAVWRPLFG
NKSS*HYDFSCFGVDF*KSFPTTMETG*TTLRLH
IHPYVSHYNQILTGGGIFFPKELYAVITRN*WE
SVLKQQVTLDHLSHTLTLPQHLHLLLFHLNSYS
RPPFYNRS*CKFFQTKSEIVLVVKQDRHPFSQSQ
HYKKIPLSQTQMFQS*ALPSHGDSGISWQLVDF
AL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK*W
KFFC*TSKAFSLKRCLVYLKVTWDIWCIQKVKSF
LTSPLRTQH*LLRYSRLP*RITALAPSFTVQQL
Y*LC*LRIGI*SPLNVTILFSKPGVKPIKYGLN
KDN**CEMIPPVKGIVTMWIER*VH*LSSNPMYS
L*HVASDRKNNSQI*SLFLSPDEE*IGQKVDDR
KEEEPE*PTVISSAVQSGSAEAHGTHTEKGNTC
CLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P*LR
NEVGRGTFNLEEEINGRL**EKYRSRKKIIFFL
*CWPRTPLHNTVLLLESIRANRVIPNSATGKTV
L*PSFPGEREQLVIFKCQNFSSK*YIH*PVSSGG
AKNTHHSEQYHYSISPLER*QRGRITWSQLLVF
N**QGLANTPENNFTLSSQGSSSLVWAPVV*PS
NVEDLMVLVCGDAGLKFNSLAKRKGRWDVLIL*W
HALSSSPWSSLYI*GN*MSLFCTIQQVLSSQGY
NYHEVLWNVVGGFSL
Frame 1
KEEARGAP*GGSG*STVSARLGDAASARTRPRPRGWRVILKVKKTSSGILPGTSPRSGTAWTVASL
RAW*TAWPCSSGTPAGRRRTGTGTWPLPWSSCCR
PTLETWRRRRPCWCWPCCWPRRWPVTRRPCSVM
SFTQQ*ILLTRTYAPT*GA*PEMGWTERTVPEV
*LAKARCGHSCIAASSVDGALACQQRS*RRQAGR
*L*LLF*RQC*TYNPL*NIYINILE*KCPFTRI
*MAFISSTHESAHL*IYTRCLYFHCAGSHLKIK
LESRFQGSRNKIQFFW*KKITVY*STTIEDGLTA
GSILFEESKNQRRNIPLTNEKFHSK*GLSILIL
RNQQSSCLKNHMAQRF*TKVLHSNDGKSGPSTQ
GLDFFNFVSFVGVKKNHLWF*KV*R*FKGPGQPL
FVYKIR*LTAYTFSLSMTSRLC*RHEATGARSY
CDAPGVSPLVIAVNFQFLAIRSAHPCLRSASGE
NRFLSIKDERAVGLFKNSAKWFPLLGTVKTKSLR
*YQNK*GKA*L*KQCNTGLLFQW*AMSQESV*K
HDSGCWVHITHIAVNRKLL*PQHEATGDAYE*G
H*RTHDFFLPDAFLFFKSLKSSERKNKLYIVQHA
IHTTLGLAQLKVGKSLQILKRSAAAQCGGHCLE
INLPNTTTFHVLEWTFKKVFQPQWKQVEPHCAY
TSIHTSAIIIRFSLGEAFSSQRSCMQSSPGTNGK
VFLSSK*LWTTSLTH*LFHSIFTCFCFT*IHTV
GHHFITGLSVNSSRPSLR*SWL*SKTVTPSPKA
NIIKRFHCLKHKCSSLEHFPAMVTRAYPGSWWIL
PCNTILVDMIQR*L*FRECVFLSVLKIFELSDG
NSFVKHQKLSP*KDAWCI*KSRGTFGAFRRSNH
S*PLLLGPSINCCDTQGFLKGSQHWRLVLLSSNF
ISCAS*ELEYRVL*MLRSCFQNLESNQLSMDLT
KTIDDVK*YHLSRA*LPCG*KDESTDYLQIPCI
LSSTLLLTERITHRFEVCSFPLMKSRLAKR*MTG
RKRSQNDPQ*SVQQCRAAVQRLMELTRKKETHA
A*NAR*LNYPNYHSIPVFLR*KHLSQH*IDLN*
GMR*EEALLIWRKK*MEDCNRKNTGLGKK*FFSF
DAGQGPLYIIQFSCWKASGQTELFQTLQQGRLY
FDLLFLGKGNN**SLNVKTFLLNSTFINQFLLV
VQRTLTTVSNTTIQFPLWRGDSGVESPGVSC*SL
TDDKALQIPLRTISL*AHREAAVWCGLLWCNHQ
MWKT*WC*CVGMPG*NSTALQKEREDGMC*YCS
GMHFPALHGALFIYEVIECHYSVPYSRYYLAKVI
ITMKCCGM**EAFHW
Frame 2
RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAPAAGG*F*KSRRHHPEYCQAPRPGRGQHGP*HPS
GPGERPGPAAQEHQPVGGGPEQGPGHCPGAAAA
GLP*RHGEGEDHAGAGPAAGQEGGQSHAVLAP*C
LSHNSEFY*PEPTHLREELSQKWDGLNGQFQKC
DWLKLDVVTAV*LLPV*TEPWHVNSVPREDRLE
DSCDFYFKDNVKLITHFKISTLIYLNENVHLHVF
EWPSYHPHMNLHICKSTHGAFISTVQVPT*KLN
WKAGFKEVETKYNFFGKKKLLFIKVQP*RMVLQ
QAVSCLRKARIREGTYPLQMKNSTQNRDYLS*Y*
GTNNLPV*KTTWHRDSELKCCTQMMGSPAPVHR
GLTFSTSFPLLESKRTTCGSKRCEGDLRAQVSH
CLFTKSGN*LHTLFLFP*HQDFAKDMKPRVPEAT
AMPRELAPW**L*TSNF*PYAQLIHASEVHLER
TGF*A*KMKEQLDFLKIQQSGSLS*GQSKPSHL
GSTKINKEKLSFRNSATLVCCSSGKLCPRNQFKS
TTVDAGSISHTLL*TGNSCDHNMRPLETHMSKG
TDGLMISSYQMLSCSLRV*NHQKGKTNSILFSM
QYIPR*GWLN*KWAKAYKY*KEVLPRSVEATVWK
*IFLTLRLFMFWSGLLKKFSNHNGNRLNHTAPT
HPSIRQPL*SDSHWGRHFLPKGAVCSHHPELMG
KCS*AASDSGPPLSHTDSSTASSPASVSLEFIQ*
ATIL*QVLV*ILPDQV*DSLGCKARPSPLLPKP
TL*KDSIVSNTNVPVLSTSQPW*LGHILAVGGF
CPVTQSLWI*FKGDFNSENVFSFLF*KYLN*VME
ILLLNIKSFLLEKMLGVSESHVGHLVHSEGQII
PDLSS*DPALTAAILKASLKDHSTGA*FYCPAT
LLVVLAENWNIESFECYDLVFKTWSQTN*VWT*Q
RQLMM*NDTTCQGHSYHVDRKMSPLIIFKSHVF
SLARCF*QKE*LTDLKFVPFP**RVDWPKGR*Q
EGRGARMTHSDQFSSAERQCRGSWNSHGKRKHML
LKMHDNLIIQITIQFRYF*DESI*ANTKLTLIK
E*GRKRHF*SGGRNKWKTVIGKIQV*EKNNFFP
LMLAKDPST*YSSPAGKHQGKQSYSKLCNREDCT
LTFFSWGKGTTSNL*MSKLFF*IVHSLTSFFWW
CKEHSPQ*AIPLFNFPFGEVTAG*NHLESAASL
*LMTRPCKYP*EQFHSELTGKQQSGVGSCGVTIK
CGRPDGVSVWGCRAEIQQPCKKKGKMGCVNIVV
ACTFQLSMELSLYMR*LNVTILYHTAGII*PRL
*LP*SAVECSRRLFTG
Sequence BID.eAug05
GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCCCGCGGCTGGAGGGTGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCT
TCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCA
CTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGT
AGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGC
AGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTT
GCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGT
TCCAGAAGTGTGACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGA
CAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCAT
TTACACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTT
AAAAATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGAT
GGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTA
TCTTAATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCC
CAGTACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCA
GGTCAGCCACTGTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTG
CCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGC
ATCTGGAGAGAACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACC
AAGTCACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAAT
CAGTTTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATA
TGAGTAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATT
GTTCAGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCC
ACTGTTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACA
CTGCGCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGA
ACTAATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAA
TTCATACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTC
TCCCAAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGT
TGGTGGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAAT
TAAGTGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAG
GTCAAATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTG
TCCAGCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTA
TGGACTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAAT
CCCATGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGG
TAGATGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGG
AAACACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGA
CCTTAATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAA
TTTTTTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGG
GAAGACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTT
TCTTCTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGC
TGCTAGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGT
GTAACCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTA
ATATTGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTA
GCCAAGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG
- BID.u.f.10 (forward primer)
- BID.u.r.14 (reverse primer)
- Single exon skipping
Frame 0
EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAPPRLEGGQQRFQPQG*VHHKPTGVWLPPKLF*QQL
PQRAGRTGPRAASAGSPVGGLR*AAD*WQPQQP
LPLGKNRGRF*KSRRHHPEYCQAPRPGRGQHGP
*HPSGPGERPGPAAQEHQPVGGGPEQGPGHCPGA
AAAGLP*RHGEGEDHAGAGPAAGQEGGQSHAVL
AP*CLSHNSEFY*PEPTHLREELSQKWDGLNGQ
FQKCDWLKLDVVTAV*LLPV*TEPWHVNSVPRED
RLEDSCDFYFKDNVKLITHFKISTLIYLNENVH
LHVFEWPSYHPHMNLHICKSTHGAFISTVQVPT
*KLNWKAGFKEVETKYNFFGKKKLLFIKVQP*RM
VLQQAVSCLRKARIREGTYPLQMKNSTQNRDYL
S*Y*GTNNLPV*KTTWHRDSELKCCTQMMGSPA
PVHRGLTFSTSFPLLESKRTTCGSKRCEGDLRAQ
VSHCLFTKSGN*LHTLFLFP*HQDFAKDMKPRV
PEATAMPRELAPW**L*TSNF*PYAQLIHASEV
HLERTGF*A*KMKEQLDFLKIQQSGSLS*GQSKP
SHLGSTKINKEKLSFRNSATLVCCSSGKLCPRN
QFKSTTVDAGSISHTLL*TGNSCDHNMRPLETH
MSKGTDGLMISSYQMLSCSLRV*NHQKGKTNSIL
FSMQYIPR*GWLN*KWAKAYKY*KEVLPRSVEA
TVWK*IFLTLRLFMFWSGLLKKFSNHNGNRLNH
TAPTHPSIRQPL*SDSHWGRHFLPKGAVCSHHPE
LMGKCS*AASDSGPPLSHTDSSTASSPASVSLE
FIQ*ATIL*QVLV*ILPDQV*DSLGCKARPSPL
LPKPTL*KDSIVSNTNVPVLSTSQPW*LGHILAV
GGFCPVTQSLWI*FKGDFNSENVFSFLF*KYLN
*VMEILLLNIKSFLLEKMLGVSESHVGHLVHSE
GQIIPDLSS*DPALTAAILKASLKDHSTGA*FYC
PATLLVVLAENWNIESFECYDLVFKTWSQTN*V
WT*QRQLMM*NDTTCQGHSYHVDRKMSPLIIFK
SHVFSLARCF*QKE*LTDLKFVPFP**RVDWPKG
R*QEGRGARMTHSDQFSSAERQCRGSWNSHGKR
KHMLLKMHDNLIIQITIQFRYF*DESI*ANTKL
TLIKE*GRKRHF*SGGRNKWKTVIGKIQV*EKNN
FFPLMLAKDPST*YSSPAGKHQGKQSYSKLCNR
EDCTLTFFSWGKGTTSNL*MSKLFF*IVHSLTS
FFWWCKEHSPQ*AIPLFNFPFGEVTAG*NHLESA
ASL*LMTRPCKYP*EQFHSELTGKQQSGVGSCG
VTIKCGRPDGVSVWGCRAEIQQPCKKKGKMGCV
NIVVACTFQLSMELSLYMR*LNVTILYHTAGII*
PRL*LP*SAVECSRRLFTG
Frame 1
KEEARGAP*GGSG*STVSARLGDAASARTRPRPRGWRVVNNGSSLRDECITNLLVFGFLQSCSDNS
FRRELDALGHELPVLAPQWEGYDELQTDGNRSSH
SRLGRIEADSESQEDIIRNIARHLAQVGDSMDR
SIPPGLVNGLALQLRNTSRSEEDRNRDLATALE
QLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSL
LRDVFHTTVNFINQNLRTYVRSLARNGMD*TDS
SRSVTG*SSMWSQLYSCFQCRRSPGMSTAFLEK
TGWKIAVTSILKTMLNL*PTLKYLH*YT*MKMSI
YTYLNGLHIIHT*ICTSVNLHTVPLFPLCRFPL
KN*IGKQVSRK*KQNTIFLVKKNYCLLKYNHRG
WSYSRQYPV*GKQESEKEHTPYK*KIPLKIGTIY
LNTKEPTIFLFKKPHGTEILN*SAALK*WEVRP
QYTGA*LFQLRFLCWSQKEPLVVLKGVKVI*GP
RSATVCLQNQVTNCIHFFSFHDIKTLLKT*SHGC
QKLLRCPGS*PPGNSCKLPISSHTLSSSMPQKC
IWREQVSKHKR*KSSWTF*KFSKVVPSLRDSQN
QVT*VVPK*IRKSLALETVQHWSAVPVVSYVPGI
SLKARQWMLGPYHTHCCEQETPVTTT*GHWRRI
*VRALTDS*FLLTRCFPVL*EFKIIRKEKQTLY
CSACNTYHARAGSIESGQKLTNTKKKCCRAVWRP
LFGNKSS*HYDFSCFGVDF*KSFPTTMETG*TT
LRLHIHPYVSHYNQILTGGGIFFPKELYAVITR
N*WESVLKQQVTLDHLSHTLTLPQHLHLLLFHLN
SYSRPPFYNRS*CKFFQTKSEIVLVVKQDRHPF
SQSQHYKKIPLSQTQMFQS*ALPSHGDSGISWQ
LVDFAL*HNPCGYDSKVTLIQRMCFPFCFKNI*I
K*WKFFC*TSKAFSLKRCLVYLKVTWDIWCIQK
VKSFLTSPLRTQH*LLRYSRLP*RITALAPSFT
VQQLY*LC*LRIGI*SPLNVTILFSKPGVKPIKY
GLNKDN**CEMIPPVKGIVTMWIER*VH*LSSN
PMYSL*HVASDRKNNSQI*SLFLSPDEE*IGQK
VDDRKEEEPE*PTVISSAVQSGSAEAHGTHTEKG
NTCCLKCTIT*LSKLPFNSGIFEMKAFEPTLN*
P*LRNEVGRGTFNLEEEINGRL**EKYRSRKKI
IFFL*CWPRTPLHNTVLLLESIRANRVIPNSATG
KTVL*PSFPGEREQLVIFKCQNFSSK*YIH*PV
SSGGAKNTHHSEQYHYSISPLER*QRGRITWSQ
LLVFN**QGLANTPENNFTLSSQGSSSLVWAPVV
*PSNVEDLMVLVCGDAGLKFNSLAKRKGRWDVL
IL*WHALSSSPWSSLYI*GN*MSLFCTIQQVLS
SQGYNYHEVLWNVVGGFSL
Frame 2
RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAPAAGGWSTTVPASGMSASQTYWCLASSKAVLTTA
SAESWTHWATSCQCWLPSGRATMSCRLMATAAA
TPAWEE*RQILKVKKTSSGILPGTSPRSGTAWTV
ASLRAW*TAWPCSSGTPAGRRRTGTGTWPLPWS
SCCRPTLETWRRRRPCWCWPCCWPRRWPVTRRP
CSVMSFTQQ*ILLTRTYAPT*GA*PEMGWTERTV
PEV*LAKARCGHSCIAASSVDGALACQQRS*RR
QAGR*L*LLF*RQC*TYNPL*NIYINILE*KCP
FTRI*MAFISSTHESAHL*IYTRCLYFHCAGSHL
KIKLESRFQGSRNKIQFFW*KKITVY*STTIED
GLTAGSILFEESKNQRRNIPLTNEKFHSK*GLS
ILILRNQQSSCLKNHMAQRF*TKVLHSNDGKSGP
STQGLDFFNFVSFVGVKKNHLWF*KV*R*FKGP
GQPLFVYKIR*LTAYTFSLSMTSRLC*RHEATG
ARSYCDAPGVSPLVIAVNFQFLAIRSAHPCLRSA
SGENRFLSIKDERAVGLFKNSAKWFPLLGTVKT
KSLR*YQNK*GKA*L*KQCNTGLLFQW*AMSQE
SV*KHDSGCWVHITHIAVNRKLL*PQHEATGDAY
E*GH*RTHDFFLPDAFLFFKSLKSSERKNKLYI
VQHAIHTTLGLAQLKVGKSLQILKRSAAAQCGG
HCLEINLPNTTTFHVLEWTFKKVFQPQWKQVEPH
CAYTSIHTSAIIIRFSLGEAFSSQRSCMQSSPG
TNGKVFLSSK*LWTTSLTH*LFHSIFTCFCFT*
IHTVGHHFITGLSVNSSRPSLR*SWL*SKTVTPS
PKANIIKRFHCLKHKCSSLEHFPAMVTRAYPGS
WWILPCNTILVDMIQR*L*FRECVFLSVLKIFE
LSDGNSFVKHQKLSP*KDAWCI*KSRGTFGAFRR
SNHS*PLLLGPSINCCDTQGFLKGSQHWRLVLL
SSNFISCAS*ELEYRVL*MLRSCFQNLESNQLS
MDLTKTIDDVK*YHLSRA*LPCG*KDESTDYLQI
PCILSSTLLLTERITHRFEVCSFPLMKSRLAKR
*MTGRKRSQNDPQ*SVQQCRAAVQRLMELTRKK
ETHAA*NAR*LNYPNYHSIPVFLR*KHLSQH*ID
LN*GMR*EEALLIWRKK*MEDCNRKNTGLGKK*
FFSFDAGQGPLYIIQFSCWKASGQTELFQTLQQ
GRLYFDLLFLGKGNN**SLNVKTFLLNSTFINQF
LLVVQRTLTTVSNTTIQFPLWRGDSGVESPGVS
C*SLTDDKALQIPLRTISL*AHREAAVWCGLLW
CNHQMWKT*WC*CVGMPG*NSTALQKEREDGMC*
YCSGMHFPALHGALFIYEVIECHYSVPYSRYYL
AKVIITMKCCGM**EAFHW
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