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Sequences

Sequence BID.iAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCT TCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCA CTCCCGCTTGGGAAGAATAGAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCA TCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCT GCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTC CGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCA GAAGTGTGACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGG CTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTAC ACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAA ATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTC TTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTT AATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGT ACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTC AGCCACTGTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAG AAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCT GGAGAGAACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGT CACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGT TTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAG TAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTC AGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTG TTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGC GCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTA ATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCA TACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCC AAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGT GGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAG TGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCA AATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCA GCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGA CTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCA TGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGA TGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAAC ACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTT AATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTT TTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAG ACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTT CTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCT AGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAA CCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATAT TGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCA AGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Alternative donor

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGQQRFQPQG*VHHKPTGVWLPPKLF*QQL PQRAGRTGPRAASAGSPVGGLR*AAD*WQPQQP LPLGKNRDSESQEDIIRNIARHLAQVGDSMDRS IPPGLVNGLALQLRNTSRSEEDRNRDLATALEQL LQAYPRDMEKEKTMLVLALLLAKKVASHTPSLL RDVFHTTVNFINQNLRTYVRSLARNGMD*TDSS RSVTG*SSMWSQLYSCFQCRRSPGMSTAFLEKTG WKIAVTSILKTMLNL*PTLKYLH*YT*MKMSIY TYLNGLHIIHT*ICTSVNLHTVPLFPLCRFPLK N*IGKQVSRK*KQNTIFLVKKNYCLLKYNHRGWS YSRQYPV*GKQESEKEHTPYK*KIPLKIGTIYL NTKEPTIFLFKKPHGTEILN*SAALK*WEVRPQ YTGA*LFQLRFLCWSQKEPLVVLKGVKVI*GPRS ATVCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQ KLLRCPGS*PPGNSCKLPISSHTLSSSMPQKCI WREQVSKHKR*KSSWTF*KFSKVVPSLRDSQNQV T*VVPK*IRKSLALETVQHWSAVPVVSYVPGIS LKARQWMLGPYHTHCCEQETPVTTT*GHWRRI* VRALTDS*FLLTRCFPVL*EFKIIRKEKQTLYCS ACNTYHARAGSIESGQKLTNTKKKCCRAVWRPL FGNKSS*HYDFSCFGVDF*KSFPTTMETG*TTL RLHIHPYVSHYNQILTGGGIFFPKELYAVITRN* WESVLKQQVTLDHLSHTLTLPQHLHLLLFHLNS YSRPPFYNRS*CKFFQTKSEIVLVVKQDRHPFS QSQHYKKIPLSQTQMFQS*ALPSHGDSGISWQLV DFAL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK *WKFFC*TSKAFSLKRCLVYLKVTWDIWCIQKV KSFLTSPLRTQH*LLRYSRLP*RITALAPSFTVQ QLY*LC*LRIGI*SPLNVTILFSKPGVKPIKYG LNKDN**CEMIPPVKGIVTMWIER*VH*LSSNP MYSL*HVASDRKNNSQI*SLFLSPDEE*IGQKVD DRKEEEPE*PTVISSAVQSGSAEAHGTHTEKGN TCCLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P *LRNEVGRGTFNLEEEINGRL**EKYRSRKKIIF FL*CWPRTPLHNTVLLLESIRANRVIPNSATGK TVL*PSFPGEREQLVIFKCQNFSSK*YIH*PVS SGGAKNTHHSEQYHYSISPLER*QRGRITWSQLL VFN**QGLANTPENNFTLSSQGSSSLVWAPVV* PSNVEDLMVLVCGDAGLKFNSLAKRKGRWDVLI L*WHALSSSPWSSLYI*GN*MSLFCTIQQVLSSQ GYNYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVNNGSSLRDECITNLLVFGFLQSCSDNS FRRELDALGHELPVLAPQWEGYDELQTDGNRSSH SRLGRIEILKVKKTSSGILPGTSPRSGTAWTVA SLRAW*TAWPCSSGTPAGRRRTGTGTWPLPWSS CCRPTLETWRRRRPCWCWPCCWPRRWPVTRRPCS VMSFTQQ*ILLTRTYAPT*GA*PEMGWTERTVP EV*LAKARCGHSCIAASSVDGALACQQRS*RRQ AGR*L*LLF*RQC*TYNPL*NIYINILE*KCPFT RI*MAFISSTHESAHL*IYTRCLYFHCAGSHLK IKLESRFQGSRNKIQFFW*KKITVY*STTIEDG LTAGSILFEESKNQRRNIPLTNEKFHSK*GLSIL ILRNQQSSCLKNHMAQRF*TKVLHSNDGKSGPS TQGLDFFNFVSFVGVKKNHLWF*KV*R*FKGPG QPLFVYKIR*LTAYTFSLSMTSRLC*RHEATGAR SYCDAPGVSPLVIAVNFQFLAIRSAHPCLRSAS GENRFLSIKDERAVGLFKNSAKWFPLLGTVKTK SLR*YQNK*GKA*L*KQCNTGLLFQW*AMSQESV *KHDSGCWVHITHIAVNRKLL*PQHEATGDAYE *GH*RTHDFFLPDAFLFFKSLKSSERKNKLYIV QHAIHTTLGLAQLKVGKSLQILKRSAAAQCGGHC LEINLPNTTTFHVLEWTFKKVFQPQWKQVEPHC AYTSIHTSAIIIRFSLGEAFSSQRSCMQSSPGT NGKVFLSSK*LWTTSLTH*LFHSIFTCFCFT*IH TVGHHFITGLSVNSSRPSLR*SWL*SKTVTPSP KANIIKRFHCLKHKCSSLEHFPAMVTRAYPGSW WILPCNTILVDMIQR*L*FRECVFLSVLKIFELS DGNSFVKHQKLSP*KDAWCI*KSRGTFGAFRRS NHS*PLLLGPSINCCDTQGFLKGSQHWRLVLLS SNFISCAS*ELEYRVL*MLRSCFQNLESNQLSMD LTKTIDDVK*YHLSRA*LPCG*KDESTDYLQIP CILSSTLLLTERITHRFEVCSFPLMKSRLAKR* MTGRKRSQNDPQ*SVQQCRAAVQRLMELTRKKET HAA*NAR*LNYPNYHSIPVFLR*KHLSQH*IDL N*GMR*EEALLIWRKK*MEDCNRKNTGLGKK*F FSFDAGQGPLYIIQFSCWKASGQTELFQTLQQGR LYFDLLFLGKGNN**SLNVKTFLLNSTFINQFL LVVQRTLTTVSNTTIQFPLWRGDSGVESPGVSC *SLTDDKALQIPLRTISL*AHREAAVWCGLLWCN HQMWKT*WC*CVGMPG*NSTALQKEREDGMC*Y CSGMHFPALHGALFIYEVIECHYSVPYSRYYLA KVIITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSTTVPASGMSASQTYWCLASSKAVLTTA SAESWTHWATSCQCWLPSGRATMSCRLMATAAA TPAWEE*RF*KSRRHHPEYCQAPRPGRGQHGP*H PSGPGERPGPAAQEHQPVGGGPEQGPGHCPGAA AAGLP*RHGEGEDHAGAGPAAGQEGGQSHAVLA P*CLSHNSEFY*PEPTHLREELSQKWDGLNGQFQ KCDWLKLDVVTAV*LLPV*TEPWHVNSVPREDR LEDSCDFYFKDNVKLITHFKISTLIYLNENVHL HVFEWPSYHPHMNLHICKSTHGAFISTVQVPT*K LNWKAGFKEVETKYNFFGKKKLLFIKVQP*RMV LQQAVSCLRKARIREGTYPLQMKNSTQNRDYLS *Y*GTNNLPV*KTTWHRDSELKCCTQMMGSPAPV HRGLTFSTSFPLLESKRTTCGSKRCEGDLRAQV SHCLFTKSGN*LHTLFLFP*HQDFAKDMKPRVP EATAMPRELAPW**L*TSNF*PYAQLIHASEVHL ERTGF*A*KMKEQLDFLKIQQSGSLS*GQSKPS HLGSTKINKEKLSFRNSATLVCCSSGKLCPRNQ FKSTTVDAGSISHTLL*TGNSCDHNMRPLETHMS KGTDGLMISSYQMLSCSLRV*NHQKGKTNSILF SMQYIPR*GWLN*KWAKAYKY*KEVLPRSVEAT VWK*IFLTLRLFMFWSGLLKKFSNHNGNRLNHTA PTHPSIRQPL*SDSHWGRHFLPKGAVCSHHPEL MGKCS*AASDSGPPLSHTDSSTASSPASVSLEF IQ*ATIL*QVLV*ILPDQV*DSLGCKARPSPLLP KPTL*KDSIVSNTNVPVLSTSQPW*LGHILAVG GFCPVTQSLWI*FKGDFNSENVFSFLF*KYLN* VMEILLLNIKSFLLEKMLGVSESHVGHLVHSEGQ IIPDLSS*DPALTAAILKASLKDHSTGA*FYCP ATLLVVLAENWNIESFECYDLVFKTWSQTN*VW T*QRQLMM*NDTTCQGHSYHVDRKMSPLIIFKSH VFSLARCF*QKE*LTDLKFVPFP**RVDWPKGR *QEGRGARMTHSDQFSSAERQCRGSWNSHGKRK HMLLKMHDNLIIQITIQFRYF*DESI*ANTKLTL IKE*GRKRHF*SGGRNKWKTVIGKIQV*EKNNF FPLMLAKDPST*YSSPAGKHQGKQSYSKLCNRE DCTLTFFSWGKGTTSNL*MSKLFF*IVHSLTSFF WWCKEHSPQ*AIPLFNFPFGEVTAG*NHLESAA SL*LMTRPCKYP*EQFHSELTGKQQSGVGSCGV TIKCGRPDGVSVWGCRAEIQQPCKKKGKMGCVNI VVACTFQLSMELSLYMR*LNVTILYHTAGII*P RL*LP*SAVECSRRLFTG

Sequence BID.eAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCT TCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCA CTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGT AGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGC AGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTT GCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGT TCCAGAAGTGTGACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGA CAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCAT TTACACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTT AAAAATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGAT GGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTA TCTTAATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCC CAGTACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCA GGTCAGCCACTGTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTG CCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGC ATCTGGAGAGAACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACC AAGTCACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAAT CAGTTTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATA TGAGTAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATT GTTCAGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCC ACTGTTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACA CTGCGCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGA ACTAATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAA TTCATACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTC TCCCAAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGT TGGTGGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAAT TAAGTGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAG GTCAAATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTG TCCAGCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTA TGGACTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAAT CCCATGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGG TAGATGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGG AAACACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGA CCTTAATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAA TTTTTTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGG GAAGACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTT TCTTCTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGC TGCTAGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGT GTAACCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTA ATATTGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTA GCCAAGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Alternative donor

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGQQRFQPQG*VHHKPTGVWLPPKLF*QQL PQRAGRTGPRAASAGSPVGGLR*AAD*WQPQQP LPLGKNRGRF*KSRRHHPEYCQAPRPGRGQHGP *HPSGPGERPGPAAQEHQPVGGGPEQGPGHCPGA AAAGLP*RHGEGEDHAGAGPAAGQEGGQSHAVL AP*CLSHNSEFY*PEPTHLREELSQKWDGLNGQ FQKCDWLKLDVVTAV*LLPV*TEPWHVNSVPRED RLEDSCDFYFKDNVKLITHFKISTLIYLNENVH LHVFEWPSYHPHMNLHICKSTHGAFISTVQVPT *KLNWKAGFKEVETKYNFFGKKKLLFIKVQP*RM VLQQAVSCLRKARIREGTYPLQMKNSTQNRDYL S*Y*GTNNLPV*KTTWHRDSELKCCTQMMGSPA PVHRGLTFSTSFPLLESKRTTCGSKRCEGDLRAQ VSHCLFTKSGN*LHTLFLFP*HQDFAKDMKPRV PEATAMPRELAPW**L*TSNF*PYAQLIHASEV HLERTGF*A*KMKEQLDFLKIQQSGSLS*GQSKP SHLGSTKINKEKLSFRNSATLVCCSSGKLCPRN QFKSTTVDAGSISHTLL*TGNSCDHNMRPLETH MSKGTDGLMISSYQMLSCSLRV*NHQKGKTNSIL FSMQYIPR*GWLN*KWAKAYKY*KEVLPRSVEA TVWK*IFLTLRLFMFWSGLLKKFSNHNGNRLNH TAPTHPSIRQPL*SDSHWGRHFLPKGAVCSHHPE LMGKCS*AASDSGPPLSHTDSSTASSPASVSLE FIQ*ATIL*QVLV*ILPDQV*DSLGCKARPSPL LPKPTL*KDSIVSNTNVPVLSTSQPW*LGHILAV GGFCPVTQSLWI*FKGDFNSENVFSFLF*KYLN *VMEILLLNIKSFLLEKMLGVSESHVGHLVHSE GQIIPDLSS*DPALTAAILKASLKDHSTGA*FYC PATLLVVLAENWNIESFECYDLVFKTWSQTN*V WT*QRQLMM*NDTTCQGHSYHVDRKMSPLIIFK SHVFSLARCF*QKE*LTDLKFVPFP**RVDWPKG R*QEGRGARMTHSDQFSSAERQCRGSWNSHGKR KHMLLKMHDNLIIQITIQFRYF*DESI*ANTKL TLIKE*GRKRHF*SGGRNKWKTVIGKIQV*EKNN FFPLMLAKDPST*YSSPAGKHQGKQSYSKLCNR EDCTLTFFSWGKGTTSNL*MSKLFF*IVHSLTS FFWWCKEHSPQ*AIPLFNFPFGEVTAG*NHLESA ASL*LMTRPCKYP*EQFHSELTGKQQSGVGSCG VTIKCGRPDGVSVWGCRAEIQQPCKKKGKMGCV NIVVACTFQLSMELSLYMR*LNVTILYHTAGII* PRL*LP*SAVECSRRLFTG

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVNNGSSLRDECITNLLVFGFLQSCSDNS FRRELDALGHELPVLAPQWEGYDELQTDGNRSSH SRLGRIEADSESQEDIIRNIARHLAQVGDSMDR SIPPGLVNGLALQLRNTSRSEEDRNRDLATALE QLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSL LRDVFHTTVNFINQNLRTYVRSLARNGMD*TDS SRSVTG*SSMWSQLYSCFQCRRSPGMSTAFLEK TGWKIAVTSILKTMLNL*PTLKYLH*YT*MKMSI YTYLNGLHIIHT*ICTSVNLHTVPLFPLCRFPL KN*IGKQVSRK*KQNTIFLVKKNYCLLKYNHRG WSYSRQYPV*GKQESEKEHTPYK*KIPLKIGTIY LNTKEPTIFLFKKPHGTEILN*SAALK*WEVRP QYTGA*LFQLRFLCWSQKEPLVVLKGVKVI*GP RSATVCLQNQVTNCIHFFSFHDIKTLLKT*SHGC QKLLRCPGS*PPGNSCKLPISSHTLSSSMPQKC IWREQVSKHKR*KSSWTF*KFSKVVPSLRDSQN QVT*VVPK*IRKSLALETVQHWSAVPVVSYVPGI SLKARQWMLGPYHTHCCEQETPVTTT*GHWRRI *VRALTDS*FLLTRCFPVL*EFKIIRKEKQTLY CSACNTYHARAGSIESGQKLTNTKKKCCRAVWRP LFGNKSS*HYDFSCFGVDF*KSFPTTMETG*TT LRLHIHPYVSHYNQILTGGGIFFPKELYAVITR N*WESVLKQQVTLDHLSHTLTLPQHLHLLLFHLN SYSRPPFYNRS*CKFFQTKSEIVLVVKQDRHPF SQSQHYKKIPLSQTQMFQS*ALPSHGDSGISWQ LVDFAL*HNPCGYDSKVTLIQRMCFPFCFKNI*I K*WKFFC*TSKAFSLKRCLVYLKVTWDIWCIQK VKSFLTSPLRTQH*LLRYSRLP*RITALAPSFT VQQLY*LC*LRIGI*SPLNVTILFSKPGVKPIKY GLNKDN**CEMIPPVKGIVTMWIER*VH*LSSN PMYSL*HVASDRKNNSQI*SLFLSPDEE*IGQK VDDRKEEEPE*PTVISSAVQSGSAEAHGTHTEKG NTCCLKCTIT*LSKLPFNSGIFEMKAFEPTLN* P*LRNEVGRGTFNLEEEINGRL**EKYRSRKKI IFFL*CWPRTPLHNTVLLLESIRANRVIPNSATG KTVL*PSFPGEREQLVIFKCQNFSSK*YIH*PV SSGGAKNTHHSEQYHYSISPLER*QRGRITWSQ LLVFN**QGLANTPENNFTLSSQGSSSLVWAPVV *PSNVEDLMVLVCGDAGLKFNSLAKRKGRWDVL IL*WHALSSSPWSSLYI*GN*MSLFCTIQQVLS SQGYNYHEVLWNVVGGFSL

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSTTVPASGMSASQTYWCLASSKAVLTTA SAESWTHWATSCQCWLPSGRATMSCRLMATAAA TPAWEE*RQILKVKKTSSGILPGTSPRSGTAWTV ASLRAW*TAWPCSSGTPAGRRRTGTGTWPLPWS SCCRPTLETWRRRRPCWCWPCCWPRRWPVTRRP CSVMSFTQQ*ILLTRTYAPT*GA*PEMGWTERTV PEV*LAKARCGHSCIAASSVDGALACQQRS*RR QAGR*L*LLF*RQC*TYNPL*NIYINILE*KCP FTRI*MAFISSTHESAHL*IYTRCLYFHCAGSHL KIKLESRFQGSRNKIQFFW*KKITVY*STTIED GLTAGSILFEESKNQRRNIPLTNEKFHSK*GLS ILILRNQQSSCLKNHMAQRF*TKVLHSNDGKSGP STQGLDFFNFVSFVGVKKNHLWF*KV*R*FKGP GQPLFVYKIR*LTAYTFSLSMTSRLC*RHEATG ARSYCDAPGVSPLVIAVNFQFLAIRSAHPCLRSA SGENRFLSIKDERAVGLFKNSAKWFPLLGTVKT KSLR*YQNK*GKA*L*KQCNTGLLFQW*AMSQE SV*KHDSGCWVHITHIAVNRKLL*PQHEATGDAY E*GH*RTHDFFLPDAFLFFKSLKSSERKNKLYI VQHAIHTTLGLAQLKVGKSLQILKRSAAAQCGG HCLEINLPNTTTFHVLEWTFKKVFQPQWKQVEPH CAYTSIHTSAIIIRFSLGEAFSSQRSCMQSSPG TNGKVFLSSK*LWTTSLTH*LFHSIFTCFCFT* IHTVGHHFITGLSVNSSRPSLR*SWL*SKTVTPS PKANIIKRFHCLKHKCSSLEHFPAMVTRAYPGS WWILPCNTILVDMIQR*L*FRECVFLSVLKIFE LSDGNSFVKHQKLSP*KDAWCI*KSRGTFGAFRR SNHS*PLLLGPSINCCDTQGFLKGSQHWRLVLL SSNFISCAS*ELEYRVL*MLRSCFQNLESNQLS MDLTKTIDDVK*YHLSRA*LPCG*KDESTDYLQI PCILSSTLLLTERITHRFEVCSFPLMKSRLAKR *MTGRKRSQNDPQ*SVQQCRAAVQRLMELTRKK ETHAA*NAR*LNYPNYHSIPVFLR*KHLSQH*ID LN*GMR*EEALLIWRKK*MEDCNRKNTGLGKK* FFSFDAGQGPLYIIQFSCWKASGQTELFQTLQQ GRLYFDLLFLGKGNN**SLNVKTFLLNSTFINQF LLVVQRTLTTVSNTTIQFPLWRGDSGVESPGVS C*SLTDDKALQIPLRTISL*AHREAAVWCGLLW CNHQMWKT*WC*CVGMPG*NSTALQKEREDGMC* YCSGMHFPALHGALFIYEVIECHYSVPYSRYYL AKVIITMKCCGM**EAFHW

Sequence BID.cAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCGCCACTGGGACACTGTGAACCAGGAGTGAGTCGGAGCTGCCGCGCTGCCCAGGCCATGGACTGTGAGGTCAACAACGGTTCC AGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACG AGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTC TGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTG GCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGG AGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAA TTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGGCTAAAGCTCGATG TGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTT TAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATATCACACACATTGCTGTGAACAGGAAACTC CTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTT TAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAAT ACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAA GTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTT CTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTT CCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGA TAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACT TCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGA ATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATC TGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCC TTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATC TTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGT GGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTT CCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGC AGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTT TTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACT GTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATC AGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAA ACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGA GAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCA CAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACA GCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAAT GTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Alternative donor

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGRHWDTVNQE*VGAAALPRPWTVRSTTVP ASGMSASQTYWCLASSKAVLTTASAESWTHWAT SCQCWLPSGRATMSCRLMATAAATPAWEE*RQI LKVKKTSSGILPGTSPRSGTAWTVASLRAW*TAW PCSSGTPAGRRRTGTGTWPLPWSSCCRPTLETW RRRRPCWCWPCCWPRRWPVTRRPCSVMSFTQQ* ILLTRTYAPT*GA*PEMGWTERTVPEV*LAKARC GHSCIAASSVDGALACQQRS*RRQAGR*L*LLF *RQC*TYNPL*NIYINILE*KCPYHTHCCEQET PVTTT*GHWRRI*VRALTDS*FLLTRCFPVL*EF KIIRKEKQTLYCSACNTYHARAGSIESGQKLTN TKKKCCRAVWRPLFGNKSS*HYDFSCFGVDF*K SFPTTMETG*TTLRLHIHPYVSHYNQILTGGGIF FPKELYAVITRN*WESVLKQQVTLDHLSHTLTL PQHLHLLLFHLNSYSRPPFYNRS*CKFFQTKSE IVLVVKQDRHPFSQSQHYKKIPLSQTQMFQS*AL PSHGDSGISWQLVDFAL*HNPCGYDSKVTLIQR MCFPFCFKNI*IK*WKFFC*TSKAFSLKRCLVY LKVTWDIWCIQKVKSFLTSPLRTQH*LLRYSRLP *RITALAPSFTVQQLY*LC*LRIGI*SPLNVTI LFSKPGVKPIKYGLNKDN**CEMIPPVKGIVTM WIER*VH*LSSNPMYSL*HVASDRKNNSQI*SLF LSPDEE*IGQKVDDRKEEEPE*PTVISSAVQSG SAEAHGTHTEKGNTCCLKCTIT*LSKLPFNSGI FEMKAFEPTLN*P*LRNEVGRGTFNLEEEINGRL **EKYRSRKKIIFFL*CWPRTPLHNTVLLLESI RANRVIPNSATGKTVL*PSFPGEREQLVIFKCQ NFSSK*YIH*PVSSGGAKNTHHSEQYHYSISPLE R*QRGRITWSQLLVFN**QGLANTPENNFTLSS QGSSSLVWAPVV*PSNVEDLMVLVCGDAGLKFN SLAKRKGRWDVLIL*WHALSSSPWSSLYI*GN*M SLFCTIQQVLSSQGYNYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVATGTL*TRSESELPRCPGHGL*GQQRF QPQG*VHHKPTGVWLPPKLF*QQLPQRAGRTGPR AASAGSPVGGLR*AAD*WQPQQPLPLGKNRGRF *KSRRHHPEYCQAPRPGRGQHGP*HPSGPGERP GPAAQEHQPVGGGPEQGPGHCPGAAAAGLP*RHG EGEDHAGAGPAAGQEGGQSHAVLAP*CLSHNSE FY*PEPTHLREELSQKWDGLNGQFQKCDWLKLD VVTAV*LLPV*TEPWHVNSVPREDRLEDSCDFYF KDNVKLITHFKISTLIYLNENVHITHIAVNRKL L*PQHEATGDAYE*GH*RTHDFFLPDAFLFFKS LKSSERKNKLYIVQHAIHTTLGLAQLKVGKSLQI LKRSAAAQCGGHCLEINLPNTTTFHVLEWTFKK VFQPQWKQVEPHCAYTSIHTSAIIIRFSLGEAF SSQRSCMQSSPGTNGKVFLSSK*LWTTSLTH*LF HSIFTCFCFT*IHTVGHHFITGLSVNSSRPSLR *SWL*SKTVTPSPKANIIKRFHCLKHKCSSLEH FPAMVTRAYPGSWWILPCNTILVDMIQR*L*FRE CVFLSVLKIFELSDGNSFVKHQKLSP*KDAWCI *KSRGTFGAFRRSNHS*PLLLGPSINCCDTQGF LKGSQHWRLVLLSSNFISCAS*ELEYRVL*MLRS CFQNLESNQLSMDLTKTIDDVK*YHLSRA*LPC G*KDESTDYLQIPCILSSTLLLTERITHRFEVC SFPLMKSRLAKR*MTGRKRSQNDPQ*SVQQCRAA VQRLMELTRKKETHAA*NAR*LNYPNYHSIPVF LR*KHLSQH*IDLN*GMR*EEALLIWRKK*MED CNRKNTGLGKK*FFSFDAGQGPLYIIQFSCWKAS GQTELFQTLQQGRLYFDLLFLGKGNN**SLNVK TFLLNSTFINQFLLVVQRTLTTVSNTTIQFPLW RGDSGVESPGVSC*SLTDDKALQIPLRTISL*AH REAAVWCGLLWCNHQMWKT*WC*CVGMPG*NST ALQKEREDGMC*YCSGMHFPALHGALFIYEVIE CHYSVPYSRYYLAKVIITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSPLGHCEPGVSRSCRAAQAMDCEVNNGS SLRDECITNLLVFGFLQSCSDNSFRRELDALGH ELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADS ESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGL ALQLRNTSRSEEDRNRDLATALEQLLQAYPRDM EKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVN FINQNLRTYVRSLARNGMD*TDSSRSVTG*SSM WSQLYSCFQCRRSPGMSTAFLEKTGWKIAVTSI LKTMLNL*PTLKYLH*YT*MKMSISHTLL*TGNS CDHNMRPLETHMSKGTDGLMISSYQMLSCSLRV *NHQKGKTNSILFSMQYIPR*GWLN*KWAKAYK Y*KEVLPRSVEATVWK*IFLTLRLFMFWSGLLKK FSNHNGNRLNHTAPTHPSIRQPL*SDSHWGRHF LPKGAVCSHHPELMGKCS*AASDSGPPLSHTDS STASSPASVSLEFIQ*ATIL*QVLV*ILPDQV*D SLGCKARPSPLLPKPTL*KDSIVSNTNVPVLST SQPW*LGHILAVGGFCPVTQSLWI*FKGDFNSE NVFSFLF*KYLN*VMEILLLNIKSFLLEKMLGVS ESHVGHLVHSEGQIIPDLSS*DPALTAAILKAS LKDHSTGA*FYCPATLLVVLAENWNIESFECYD LVFKTWSQTN*VWT*QRQLMM*NDTTCQGHSYHV DRKMSPLIIFKSHVFSLARCF*QKE*LTDLKFV PFP**RVDWPKGR*QEGRGARMTHSDQFSSAER QCRGSWNSHGKRKHMLLKMHDNLIIQITIQFRYF *DESI*ANTKLTLIKE*GRKRHF*SGGRNKWKT VIGKIQV*EKNNFFPLMLAKDPST*YSSPAGKH QGKQSYSKLCNREDCTLTFFSWGKGTTSNL*MSK LFF*IVHSLTSFFWWCKEHSPQ*AIPLFNFPFG EVTAG*NHLESAASL*LMTRPCKYP*EQFHSEL TGKQQSGVGSCGVTIKCGRPDGVSVWGCRAEIQQ PCKKKGKMGCVNIVVACTFQLSMELSLYMR*LN VTILYHTAGII*PRL*LP*SAVECSRRLFTG

Sequence BID.bAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCGCCACTGGGACACTGTGAACCAGGAGTGAGTCGGAGCTGCCGCGCTGCCCAGGCCATGGACTGTGAGGTCAACAACGGTTCC AGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACG AGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTC TGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTG GCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGG AGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAA TTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGGCTAAAGCTCGATG TGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTT TAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATTTGAATGGCCTTCATATCAT CCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAATTGGAAAGCAGGTTTCAAGG AAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGCAGGCAGTATCCTGTTTGAG GAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTAAGGAACCAACAATCTTCCT GTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGGGGCTTGACTTTTTCAACTT CGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACTGTTTGTTTACAAAATCAGG TAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTACTGCGATGCCCCGGGAGTTA GCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGAACAGGTTTCTAAGCATAAA AGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAGGTAGTACCAAAATAAATAA GGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAGCACGACAGTGGATGCTGGG TCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGCACTGACGGACTCATGATTT CTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGCTAGGG CTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAAATAAATCTTCCTAACACTA CGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACACATCCATCCATACGTCAGC CATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAAAGTGTTCTTAAGCAGCAAG TGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTAGGCCACCATTTTATAACAG GTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCAACATTATAAAAAGATTCCA TTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTTGCCCTGTAACACAATCCTT GTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGAAATTCTTTTGTTAAACATC AAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATTCCTGACCTCTCCTCTTAGG ACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTTTATTAGTTGTGCTAGCTGA GAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACAAAGACAATTGATGATGTGA AATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTCTCTCTAGCACGTTGCTTCT GACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGGAAGGAAGAGGAGCCAGAAT GACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCTGCTTAAAATGCACGATAAC TTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAGGAATGAGGTAGGAAGAGGC ACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTTTGATGCTGGCCAAGGACCC CTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTACTTTGACCTTCTTTTCCTGG GGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGGTGCAAAGAACACTCACCAC AGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTTAACTGATGACAAGGCCTTG CAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAAATGTGGAAGACCTGATGGT GTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTGGCATGCACTTTCCAGCTCT CCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTATAATTACCATGAAGTGCTGT GGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Alternative donor

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGRHWDTVNQE*VGAAALPRPWTVRSTTVP ASGMSASQTYWCLASSKAVLTTASAESWTHWAT SCQCWLPSGRATMSCRLMATAAATPAWEE*RQI LKVKKTSSGILPGTSPRSGTAWTVASLRAW*TAW PCSSGTPAGRRRTGTGTWPLPWSSCCRPTLETW RRRRPCWCWPCCWPRRWPVTRRPCSVMSFTQQ* ILLTRTYAPT*GA*PEMGWTERTVPEV*LAKARC GHSCIAASSVDGALACQQRS*RRQAGR*L*LLF *RQC*TYNPL*NIYINILE*KCPFTRI*MAFIS STHESAHL*IYTRCLYFHCAGSHLKIKLESRFQG SRNKIQFFW*KKITVY*STTIEDGLTAGSILFE ESKNQRRNIPLTNEKFHSK*GLSILILRNQQSS CLKNHMAQRF*TKVLHSNDGKSGPSTQGLDFFNF VSFVGVKKNHLWF*KV*R*FKGPGQPLFVYKIR *LTAYTFSLSMTSRLC*RHEATGARSYCDAPGV SPLVIAVNFQFLAIRSAHPCLRSASGENRFLSIK DERAVGLFKNSAKWFPLLGTVKTKSLR*YQNK* GKA*L*KQCNTGLLFQW*AMSQESV*KHDSGCW VHITHIAVNRKLL*PQHEATGDAYE*GH*RTHDF FLPDAFLFFKSLKSSERKNKLYIVQHAIHTTLG LAQLKVGKSLQILKRSAAAQCGGHCLEINLPNT TTFHVLEWTFKKVFQPQWKQVEPHCAYTSIHTSA IIIRFSLGEAFSSQRSCMQSSPGTNGKVFLSSK *LWTTSLTH*LFHSIFTCFCFT*IHTVGHHFIT GLSVNSSRPSLR*SWL*SKTVTPSPKANIIKRFH CLKHKCSSLEHFPAMVTRAYPGSWWILPCNTIL VDMIQR*L*FRECVFLSVLKIFELSDGNSFVKH QKLSP*KDAWCI*KSRGTFGAFRRSNHS*PLLLG PSINCCDTQGFLKGSQHWRLVLLSSNFISCAS* ELEYRVL*MLRSCFQNLESNQLSMDLTKTIDDV K*YHLSRA*LPCG*KDESTDYLQIPCILSSTLLL TERITHRFEVCSFPLMKSRLAKR*MTGRKRSQN DPQ*SVQQCRAAVQRLMELTRKKETHAA*NAR* LNYPNYHSIPVFLR*KHLSQH*IDLN*GMR*EEA LLIWRKK*MEDCNRKNTGLGKK*FFSFDAGQGP LYIIQFSCWKASGQTELFQTLQQGRLYFDLLFL GKGNN**SLNVKTFLLNSTFINQFLLVVQRTLTT VSNTTIQFPLWRGDSGVESPGVSC*SLTDDKAL QIPLRTISL*AHREAAVWCGLLWCNHQMWKT*W C*CVGMPG*NSTALQKEREDGMC*YCSGMHFPAL HGALFIYEVIECHYSVPYSRYYLAKVIITMKCC GM**EAFHW

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVATGTL*TRSESELPRCPGHGL*GQQRF QPQG*VHHKPTGVWLPPKLF*QQLPQRAGRTGPR AASAGSPVGGLR*AAD*WQPQQPLPLGKNRGRF *KSRRHHPEYCQAPRPGRGQHGP*HPSGPGERP GPAAQEHQPVGGGPEQGPGHCPGAAAAGLP*RHG EGEDHAGAGPAAGQEGGQSHAVLAP*CLSHNSE FY*PEPTHLREELSQKWDGLNGQFQKCDWLKLD VVTAV*LLPV*TEPWHVNSVPREDRLEDSCDFYF KDNVKLITHFKISTLIYLNENVHLHVFEWPSYH PHMNLHICKSTHGAFISTVQVPT*KLNWKAGFK EVETKYNFFGKKKLLFIKVQP*RMVLQQAVSCLR KARIREGTYPLQMKNSTQNRDYLS*Y*GTNNLP V*KTTWHRDSELKCCTQMMGSPAPVHRGLTFST SFPLLESKRTTCGSKRCEGDLRAQVSHCLFTKSG N*LHTLFLFP*HQDFAKDMKPRVPEATAMPREL APW**L*TSNF*PYAQLIHASEVHLERTGF*A* KMKEQLDFLKIQQSGSLS*GQSKPSHLGSTKINK EKLSFRNSATLVCCSSGKLCPRNQFKSTTVDAG SISHTLL*TGNSCDHNMRPLETHMSKGTDGLMI SSYQMLSCSLRV*NHQKGKTNSILFSMQYIPR*G WLN*KWAKAYKY*KEVLPRSVEATVWK*IFLTL RLFMFWSGLLKKFSNHNGNRLNHTAPTHPSIRQ PL*SDSHWGRHFLPKGAVCSHHPELMGKCS*AAS DSGPPLSHTDSSTASSPASVSLEFIQ*ATIL*Q VLV*ILPDQV*DSLGCKARPSPLLPKPTL*KDS IVSNTNVPVLSTSQPW*LGHILAVGGFCPVTQSL WI*FKGDFNSENVFSFLF*KYLN*VMEILLLNI KSFLLEKMLGVSESHVGHLVHSEGQIIPDLSS* DPALTAAILKASLKDHSTGA*FYCPATLLVVLAE NWNIESFECYDLVFKTWSQTN*VWT*QRQLMM* NDTTCQGHSYHVDRKMSPLIIFKSHVFSLARCF *QKE*LTDLKFVPFP**RVDWPKGR*QEGRGARM THSDQFSSAERQCRGSWNSHGKRKHMLLKMHDN LIIQITIQFRYF*DESI*ANTKLTLIKE*GRKR HF*SGGRNKWKTVIGKIQV*EKNNFFPLMLAKDP ST*YSSPAGKHQGKQSYSKLCNREDCTLTFFSW GKGTTSNL*MSKLFF*IVHSLTSFFWWCKEHSP Q*AIPLFNFPFGEVTAG*NHLESAASL*LMTRPC KYP*EQFHSELTGKQQSGVGSCGVTIKCGRPDG VSVWGCRAEIQQPCKKKGKMGCVNIVVACTFQL SMELSLYMR*LNVTILYHTAGII*PRL*LP*SAV ECSRRLFTG

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSPLGHCEPGVSRSCRAAQAMDCEVNNGS SLRDECITNLLVFGFLQSCSDNSFRRELDALGH ELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADS ESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGL ALQLRNTSRSEEDRNRDLATALEQLLQAYPRDM EKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVN FINQNLRTYVRSLARNGMD*TDSSRSVTG*SSM WSQLYSCFQCRRSPGMSTAFLEKTGWKIAVTSI LKTMLNL*PTLKYLH*YT*MKMSIYTYLNGLHII HT*ICTSVNLHTVPLFPLCRFPLKN*IGKQVSR K*KQNTIFLVKKNYCLLKYNHRGWSYSRQYPV* GKQESEKEHTPYK*KIPLKIGTIYLNTKEPTIFL FKKPHGTEILN*SAALK*WEVRPQYTGA*LFQL RFLCWSQKEPLVVLKGVKVI*GPRSATVCLQNQ VTNCIHFFSFHDIKTLLKT*SHGCQKLLRCPGS* PPGNSCKLPISSHTLSSSMPQKCIWREQVSKHK R*KSSWTF*KFSKVVPSLRDSQNQVT*VVPK*I RKSLALETVQHWSAVPVVSYVPGISLKARQWMLG PYHTHCCEQETPVTTT*GHWRRI*VRALTDS*F LLTRCFPVL*EFKIIRKEKQTLYCSACNTYHAR AGSIESGQKLTNTKKKCCRAVWRPLFGNKSS*HY DFSCFGVDF*KSFPTTMETG*TTLRLHIHPYVS HYNQILTGGGIFFPKELYAVITRN*WESVLKQQ VTLDHLSHTLTLPQHLHLLLFHLNSYSRPPFYNR S*CKFFQTKSEIVLVVKQDRHPFSQSQHYKKIP LSQTQMFQS*ALPSHGDSGISWQLVDFAL*HNP CGYDSKVTLIQRMCFPFCFKNI*IK*WKFFC*TS KAFSLKRCLVYLKVTWDIWCIQKVKSFLTSPLR TQH*LLRYSRLP*RITALAPSFTVQQLY*LC*L RIGI*SPLNVTILFSKPGVKPIKYGLNKDN**CE MIPPVKGIVTMWIER*VH*LSSNPMYSL*HVAS DRKNNSQI*SLFLSPDEE*IGQKVDDRKEEEPE *PTVISSAVQSGSAEAHGTHTEKGNTCCLKCTIT *LSKLPFNSGIFEMKAFEPTLN*P*LRNEVGRG TFNLEEEINGRL**EKYRSRKKIIFFL*CWPRT PLHNTVLLLESIRANRVIPNSATGKTVL*PSFPG EREQLVIFKCQNFSSK*YIH*PVSSGGAKNTHH SEQYHYSISPLER*QRGRITWSQLLVFN**QGL ANTPENNFTLSSQGSSSLVWAPVV*PSNVEDLMV LVCGDAGLKFNSLAKRKGRWDVLIL*WHALSSS PWSSLYI*GN*MSLFCTIQQVLSSQGYNYHEVL WNVVGGFSL