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Sequences

Sequence BID.hAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCC GGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAG GCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATG TCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGT GACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAG ATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATT TGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAAT TGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGC AGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTA AGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGG GGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACT GTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTAC TGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGA ACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAG GTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAG CACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGC ACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGC AATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAA ATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACA CATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAA AGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTA GGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCA ACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTT GCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGA AATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATT CCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTT TATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACA AAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTC TCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGG AAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCT GCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAG GAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTT TGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTAC TTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGG TGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTT AACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAA ATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTG GCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTAT AATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Single exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGDSESQEDIIRNIARHLAQVGDSMDRSIPP GLVNGLALQLRNTSRSEEDRNRDLATALEQLLQ AYPRDMEKEKTMLVLALLLAKKVASHTPSLLRD VFHTTVNFINQNLRTYVRSLARNGMD*TDSSRSV TG*SSMWSQLYSCFQCRRSPGMSTAFLEKTGWK IAVTSILKTMLNL*PTLKYLH*YT*MKMSIYTY LNGLHIIHT*ICTSVNLHTVPLFPLCRFPLKN*I GKQVSRK*KQNTIFLVKKNYCLLKYNHRGWSYS RQYPV*GKQESEKEHTPYK*KIPLKIGTIYLNT KEPTIFLFKKPHGTEILN*SAALK*WEVRPQYTG A*LFQLRFLCWSQKEPLVVLKGVKVI*GPRSAT VCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQKL LRCPGS*PPGNSCKLPISSHTLSSSMPQKCIWRE QVSKHKR*KSSWTF*KFSKVVPSLRDSQNQVT* VVPK*IRKSLALETVQHWSAVPVVSYVPGISLK ARQWMLGPYHTHCCEQETPVTTT*GHWRRI*VRA LTDS*FLLTRCFPVL*EFKIIRKEKQTLYCSAC NTYHARAGSIESGQKLTNTKKKCCRAVWRPLFG NKSS*HYDFSCFGVDF*KSFPTTMETG*TTLRLH IHPYVSHYNQILTGGGIFFPKELYAVITRN*WE SVLKQQVTLDHLSHTLTLPQHLHLLLFHLNSYS RPPFYNRS*CKFFQTKSEIVLVVKQDRHPFSQSQ HYKKIPLSQTQMFQS*ALPSHGDSGISWQLVDF AL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK*W KFFC*TSKAFSLKRCLVYLKVTWDIWCIQKVKSF LTSPLRTQH*LLRYSRLP*RITALAPSFTVQQL Y*LC*LRIGI*SPLNVTILFSKPGVKPIKYGLN KDN**CEMIPPVKGIVTMWIER*VH*LSSNPMYS L*HVASDRKNNSQI*SLFLSPDEE*IGQKVDDR KEEEPE*PTVISSAVQSGSAEAHGTHTEKGNTC CLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P*LR NEVGRGTFNLEEEINGRL**EKYRSRKKIIFFL *CWPRTPLHNTVLLLESIRANRVIPNSATGKTV L*PSFPGEREQLVIFKCQNFSSK*YIH*PVSSGG AKNTHHSEQYHYSISPLER*QRGRITWSQLLVF N**QGLANTPENNFTLSSQGSSSLVWAPVV*PS NVEDLMVLVCGDAGLKFNSLAKRKGRWDVLIL*W HALSSSPWSSLYI*GN*MSLFCTIQQVLSSQGY NYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVILKVKKTSSGILPGTSPRSGTAWTVASL RAW*TAWPCSSGTPAGRRRTGTGTWPLPWSSCCR PTLETWRRRRPCWCWPCCWPRRWPVTRRPCSVM SFTQQ*ILLTRTYAPT*GA*PEMGWTERTVPEV *LAKARCGHSCIAASSVDGALACQQRS*RRQAGR *L*LLF*RQC*TYNPL*NIYINILE*KCPFTRI *MAFISSTHESAHL*IYTRCLYFHCAGSHLKIK LESRFQGSRNKIQFFW*KKITVY*STTIEDGLTA GSILFEESKNQRRNIPLTNEKFHSK*GLSILIL RNQQSSCLKNHMAQRF*TKVLHSNDGKSGPSTQ GLDFFNFVSFVGVKKNHLWF*KV*R*FKGPGQPL FVYKIR*LTAYTFSLSMTSRLC*RHEATGARSY CDAPGVSPLVIAVNFQFLAIRSAHPCLRSASGE NRFLSIKDERAVGLFKNSAKWFPLLGTVKTKSLR *YQNK*GKA*L*KQCNTGLLFQW*AMSQESV*K HDSGCWVHITHIAVNRKLL*PQHEATGDAYE*G H*RTHDFFLPDAFLFFKSLKSSERKNKLYIVQHA IHTTLGLAQLKVGKSLQILKRSAAAQCGGHCLE INLPNTTTFHVLEWTFKKVFQPQWKQVEPHCAY TSIHTSAIIIRFSLGEAFSSQRSCMQSSPGTNGK VFLSSK*LWTTSLTH*LFHSIFTCFCFT*IHTV GHHFITGLSVNSSRPSLR*SWL*SKTVTPSPKA NIIKRFHCLKHKCSSLEHFPAMVTRAYPGSWWIL PCNTILVDMIQR*L*FRECVFLSVLKIFELSDG NSFVKHQKLSP*KDAWCI*KSRGTFGAFRRSNH S*PLLLGPSINCCDTQGFLKGSQHWRLVLLSSNF ISCAS*ELEYRVL*MLRSCFQNLESNQLSMDLT KTIDDVK*YHLSRA*LPCG*KDESTDYLQIPCI LSSTLLLTERITHRFEVCSFPLMKSRLAKR*MTG RKRSQNDPQ*SVQQCRAAVQRLMELTRKKETHA A*NAR*LNYPNYHSIPVFLR*KHLSQH*IDLN* GMR*EEALLIWRKK*MEDCNRKNTGLGKK*FFSF DAGQGPLYIIQFSCWKASGQTELFQTLQQGRLY FDLLFLGKGNN**SLNVKTFLLNSTFINQFLLV VQRTLTTVSNTTIQFPLWRGDSGVESPGVSC*SL TDDKALQIPLRTISL*AHREAAVWCGLLWCNHQ MWKT*WC*CVGMPG*NSTALQKEREDGMC*YCS GMHFPALHGALFIYEVIECHYSVPYSRYYLAKVI ITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGG*F*KSRRHHPEYCQAPRPGRGQHGP*HPS GPGERPGPAAQEHQPVGGGPEQGPGHCPGAAAA GLP*RHGEGEDHAGAGPAAGQEGGQSHAVLAP*C LSHNSEFY*PEPTHLREELSQKWDGLNGQFQKC DWLKLDVVTAV*LLPV*TEPWHVNSVPREDRLE DSCDFYFKDNVKLITHFKISTLIYLNENVHLHVF EWPSYHPHMNLHICKSTHGAFISTVQVPT*KLN WKAGFKEVETKYNFFGKKKLLFIKVQP*RMVLQ QAVSCLRKARIREGTYPLQMKNSTQNRDYLS*Y* GTNNLPV*KTTWHRDSELKCCTQMMGSPAPVHR GLTFSTSFPLLESKRTTCGSKRCEGDLRAQVSH CLFTKSGN*LHTLFLFP*HQDFAKDMKPRVPEAT AMPRELAPW**L*TSNF*PYAQLIHASEVHLER TGF*A*KMKEQLDFLKIQQSGSLS*GQSKPSHL GSTKINKEKLSFRNSATLVCCSSGKLCPRNQFKS TTVDAGSISHTLL*TGNSCDHNMRPLETHMSKG TDGLMISSYQMLSCSLRV*NHQKGKTNSILFSM QYIPR*GWLN*KWAKAYKY*KEVLPRSVEATVWK *IFLTLRLFMFWSGLLKKFSNHNGNRLNHTAPT HPSIRQPL*SDSHWGRHFLPKGAVCSHHPELMG KCS*AASDSGPPLSHTDSSTASSPASVSLEFIQ* ATIL*QVLV*ILPDQV*DSLGCKARPSPLLPKP TL*KDSIVSNTNVPVLSTSQPW*LGHILAVGGF CPVTQSLWI*FKGDFNSENVFSFLF*KYLN*VME ILLLNIKSFLLEKMLGVSESHVGHLVHSEGQII PDLSS*DPALTAAILKASLKDHSTGA*FYCPAT LLVVLAENWNIESFECYDLVFKTWSQTN*VWT*Q RQLMM*NDTTCQGHSYHVDRKMSPLIIFKSHVF SLARCF*QKE*LTDLKFVPFP**RVDWPKGR*Q EGRGARMTHSDQFSSAERQCRGSWNSHGKRKHML LKMHDNLIIQITIQFRYF*DESI*ANTKLTLIK E*GRKRHF*SGGRNKWKTVIGKIQV*EKNNFFP LMLAKDPST*YSSPAGKHQGKQSYSKLCNREDCT LTFFSWGKGTTSNL*MSKLFF*IVHSLTSFFWW CKEHSPQ*AIPLFNFPFGEVTAG*NHLESAASL *LMTRPCKYP*EQFHSELTGKQQSGVGSCGVTIK CGRPDGVSVWGCRAEIQQPCKKKGKMGCVNIVV ACTFQLSMELSLYMR*LNVTILYHTAGII*PRL *LP*SAVECSRRLFTG

Sequence BID.iAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCT TCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCA CTCCCGCTTGGGAAGAATAGAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCA TCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCT GCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTC CGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCA GAAGTGTGACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGG CTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTAC ACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAA ATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTC TTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTT AATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGT ACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTC AGCCACTGTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAG AAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCT GGAGAGAACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGT CACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGT TTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAG TAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTC AGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTG TTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGC GCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTA ATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCA TACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCC AAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGT GGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAG TGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCA AATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCA GCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGA CTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCA TGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGA TGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAAC ACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTT AATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTT TTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAG ACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTT CTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCT AGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAA CCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATAT TGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCA AGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.u.f.10 (forward primer)
BID.u.r.14 (reverse primer)
Single exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGQQRFQPQG*VHHKPTGVWLPPKLF*QQL PQRAGRTGPRAASAGSPVGGLR*AAD*WQPQQP LPLGKNRDSESQEDIIRNIARHLAQVGDSMDRS IPPGLVNGLALQLRNTSRSEEDRNRDLATALEQL LQAYPRDMEKEKTMLVLALLLAKKVASHTPSLL RDVFHTTVNFINQNLRTYVRSLARNGMD*TDSS RSVTG*SSMWSQLYSCFQCRRSPGMSTAFLEKTG WKIAVTSILKTMLNL*PTLKYLH*YT*MKMSIY TYLNGLHIIHT*ICTSVNLHTVPLFPLCRFPLK N*IGKQVSRK*KQNTIFLVKKNYCLLKYNHRGWS YSRQYPV*GKQESEKEHTPYK*KIPLKIGTIYL NTKEPTIFLFKKPHGTEILN*SAALK*WEVRPQ YTGA*LFQLRFLCWSQKEPLVVLKGVKVI*GPRS ATVCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQ KLLRCPGS*PPGNSCKLPISSHTLSSSMPQKCI WREQVSKHKR*KSSWTF*KFSKVVPSLRDSQNQV T*VVPK*IRKSLALETVQHWSAVPVVSYVPGIS LKARQWMLGPYHTHCCEQETPVTTT*GHWRRI* VRALTDS*FLLTRCFPVL*EFKIIRKEKQTLYCS ACNTYHARAGSIESGQKLTNTKKKCCRAVWRPL FGNKSS*HYDFSCFGVDF*KSFPTTMETG*TTL RLHIHPYVSHYNQILTGGGIFFPKELYAVITRN* WESVLKQQVTLDHLSHTLTLPQHLHLLLFHLNS YSRPPFYNRS*CKFFQTKSEIVLVVKQDRHPFS QSQHYKKIPLSQTQMFQS*ALPSHGDSGISWQLV DFAL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK *WKFFC*TSKAFSLKRCLVYLKVTWDIWCIQKV KSFLTSPLRTQH*LLRYSRLP*RITALAPSFTVQ QLY*LC*LRIGI*SPLNVTILFSKPGVKPIKYG LNKDN**CEMIPPVKGIVTMWIER*VH*LSSNP MYSL*HVASDRKNNSQI*SLFLSPDEE*IGQKVD DRKEEEPE*PTVISSAVQSGSAEAHGTHTEKGN TCCLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P *LRNEVGRGTFNLEEEINGRL**EKYRSRKKIIF FL*CWPRTPLHNTVLLLESIRANRVIPNSATGK TVL*PSFPGEREQLVIFKCQNFSSK*YIH*PVS SGGAKNTHHSEQYHYSISPLER*QRGRITWSQLL VFN**QGLANTPENNFTLSSQGSSSLVWAPVV* PSNVEDLMVLVCGDAGLKFNSLAKRKGRWDVLI L*WHALSSSPWSSLYI*GN*MSLFCTIQQVLSSQ GYNYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVNNGSSLRDECITNLLVFGFLQSCSDNS FRRELDALGHELPVLAPQWEGYDELQTDGNRSSH SRLGRIEILKVKKTSSGILPGTSPRSGTAWTVA SLRAW*TAWPCSSGTPAGRRRTGTGTWPLPWSS CCRPTLETWRRRRPCWCWPCCWPRRWPVTRRPCS VMSFTQQ*ILLTRTYAPT*GA*PEMGWTERTVP EV*LAKARCGHSCIAASSVDGALACQQRS*RRQ AGR*L*LLF*RQC*TYNPL*NIYINILE*KCPFT RI*MAFISSTHESAHL*IYTRCLYFHCAGSHLK IKLESRFQGSRNKIQFFW*KKITVY*STTIEDG LTAGSILFEESKNQRRNIPLTNEKFHSK*GLSIL ILRNQQSSCLKNHMAQRF*TKVLHSNDGKSGPS TQGLDFFNFVSFVGVKKNHLWF*KV*R*FKGPG QPLFVYKIR*LTAYTFSLSMTSRLC*RHEATGAR SYCDAPGVSPLVIAVNFQFLAIRSAHPCLRSAS GENRFLSIKDERAVGLFKNSAKWFPLLGTVKTK SLR*YQNK*GKA*L*KQCNTGLLFQW*AMSQESV *KHDSGCWVHITHIAVNRKLL*PQHEATGDAYE *GH*RTHDFFLPDAFLFFKSLKSSERKNKLYIV QHAIHTTLGLAQLKVGKSLQILKRSAAAQCGGHC LEINLPNTTTFHVLEWTFKKVFQPQWKQVEPHC AYTSIHTSAIIIRFSLGEAFSSQRSCMQSSPGT NGKVFLSSK*LWTTSLTH*LFHSIFTCFCFT*IH TVGHHFITGLSVNSSRPSLR*SWL*SKTVTPSP KANIIKRFHCLKHKCSSLEHFPAMVTRAYPGSW WILPCNTILVDMIQR*L*FRECVFLSVLKIFELS DGNSFVKHQKLSP*KDAWCI*KSRGTFGAFRRS NHS*PLLLGPSINCCDTQGFLKGSQHWRLVLLS SNFISCAS*ELEYRVL*MLRSCFQNLESNQLSMD LTKTIDDVK*YHLSRA*LPCG*KDESTDYLQIP CILSSTLLLTERITHRFEVCSFPLMKSRLAKR* MTGRKRSQNDPQ*SVQQCRAAVQRLMELTRKKET HAA*NAR*LNYPNYHSIPVFLR*KHLSQH*IDL N*GMR*EEALLIWRKK*MEDCNRKNTGLGKK*F FSFDAGQGPLYIIQFSCWKASGQTELFQTLQQGR LYFDLLFLGKGNN**SLNVKTFLLNSTFINQFL LVVQRTLTTVSNTTIQFPLWRGDSGVESPGVSC *SLTDDKALQIPLRTISL*AHREAAVWCGLLWCN HQMWKT*WC*CVGMPG*NSTALQKEREDGMC*Y CSGMHFPALHGALFIYEVIECHYSVPYSRYYLA KVIITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSTTVPASGMSASQTYWCLASSKAVLTTA SAESWTHWATSCQCWLPSGRATMSCRLMATAAA TPAWEE*RF*KSRRHHPEYCQAPRPGRGQHGP*H PSGPGERPGPAAQEHQPVGGGPEQGPGHCPGAA AAGLP*RHGEGEDHAGAGPAAGQEGGQSHAVLA P*CLSHNSEFY*PEPTHLREELSQKWDGLNGQFQ KCDWLKLDVVTAV*LLPV*TEPWHVNSVPREDR LEDSCDFYFKDNVKLITHFKISTLIYLNENVHL HVFEWPSYHPHMNLHICKSTHGAFISTVQVPT*K LNWKAGFKEVETKYNFFGKKKLLFIKVQP*RMV LQQAVSCLRKARIREGTYPLQMKNSTQNRDYLS *Y*GTNNLPV*KTTWHRDSELKCCTQMMGSPAPV HRGLTFSTSFPLLESKRTTCGSKRCEGDLRAQV SHCLFTKSGN*LHTLFLFP*HQDFAKDMKPRVP EATAMPRELAPW**L*TSNF*PYAQLIHASEVHL ERTGF*A*KMKEQLDFLKIQQSGSLS*GQSKPS HLGSTKINKEKLSFRNSATLVCCSSGKLCPRNQ FKSTTVDAGSISHTLL*TGNSCDHNMRPLETHMS KGTDGLMISSYQMLSCSLRV*NHQKGKTNSILF SMQYIPR*GWLN*KWAKAYKY*KEVLPRSVEAT VWK*IFLTLRLFMFWSGLLKKFSNHNGNRLNHTA PTHPSIRQPL*SDSHWGRHFLPKGAVCSHHPEL MGKCS*AASDSGPPLSHTDSSTASSPASVSLEF IQ*ATIL*QVLV*ILPDQV*DSLGCKARPSPLLP KPTL*KDSIVSNTNVPVLSTSQPW*LGHILAVG GFCPVTQSLWI*FKGDFNSENVFSFLF*KYLN* VMEILLLNIKSFLLEKMLGVSESHVGHLVHSEGQ IIPDLSS*DPALTAAILKASLKDHSTGA*FYCP ATLLVVLAENWNIESFECYDLVFKTWSQTN*VW T*QRQLMM*NDTTCQGHSYHVDRKMSPLIIFKSH VFSLARCF*QKE*LTDLKFVPFP**RVDWPKGR *QEGRGARMTHSDQFSSAERQCRGSWNSHGKRK HMLLKMHDNLIIQITIQFRYF*DESI*ANTKLTL IKE*GRKRHF*SGGRNKWKTVIGKIQV*EKNNF FPLMLAKDPST*YSSPAGKHQGKQSYSKLCNRE DCTLTFFSWGKGTTSNL*MSKLFF*IVHSLTSFF WWCKEHSPQ*AIPLFNFPFGEVTAG*NHLESAA SL*LMTRPCKYP*EQFHSELTGKQQSGVGSCGV TIKCGRPDGVSVWGCRAEIQQPCKKKGKMGCVNI VVACTFQLSMELSLYMR*LNVTILYHTAGII*P RL*LP*SAVECSRRLFTG