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Sequence AKT2.iAug05
CCATCAAGGCCAGTGGCCACCAGTTAAATGGACTAGATTGAGGATGGCTTATGTATCATTTCTTTATCTCAGTTGCTGTCCAGGTATCCTATTAAGCCACAGTACTTTTTCAAATAAAATCTTATGAGGTACCTCAGTGCAGAAAACAGAATAGTTGAGTTGTCATGAGCTGAAGGACCCCCTGGTGGGAGTCTCTGAGG
AACTCTCTGTAGATCTCCTTAGTGCAGTGGAGAACTACTGTTTGTTGACTGACAGCCTGTCCATCCTGTCATTGAAGTTGGCTCCCAAGCCTTTGCCACT
CCCAGCCACAGCAGACATCACTTGTGTGTGTAAGTTGAACACATTTTACTGTAAAAACAAGAGCCATGGTTGCTCAGAACAGCTCTAGTTCTCCCTTGGC
TGTGCCTGATGGAGGCAGAGGACAAGATGGTTCAGCATGTAACCCTTTCCTACCGCAGAGCCATGCCTTCACTTAATGCCCCAAAAGGTGCAGCCGGTAT
GTGAACCCCATATGGCTGACTCCTGGGGGCCCCAGCCAGGGCAGGGTGTGTGTGTGCAGAATAAGCAGGGCCAGGGCCCTGCCCTGGCATGGCTCAGCAG
AGCCCTCCTCCCTCCCACCTCCCACCCTGCTGCCTTCATCCCCCAGGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTAT
GCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGT
ACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAA
AGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTGAGGTGTCTGGGGTTGGTGGGGGTGCTGGAGGACAA
TGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGC
- AKT2.F10 (forward primer)
- AKT2.R10 (reverse primer)
- Intron retention
Frame 0
PSRPVATS*MD*IEDGLCIISLSQLLSRYPIKPQYFFK*NLMRYLSAENRIVELS*AEGPPGGSL*G
TLCRSP*CSGELLFVD*QPVHPVIEVGSQAFAT
PSHSRHHLCV*VEHILL*KQEPWLLRTALVLPW
LCLMEAEDKMVQHVTLSYRRAMPSLNAPKGAAGM
*TPYG*LLGAPARAGCVCAE*AGPGPCPGMAQQ
SPPPSHLPPCCLHPPGAEVCLPDPRPPVLCDGV
CQRG*AVLPPVPGACLHRGAGPVLWCRDCLGS*V
LALAGRGIPRHQAGKPHAGQRWPHQDH*LWPLQ
RGHQ*RGHHENLLWDPGVPGA*GVWGWWGCWRT
MTMAGPWTGGGWVWSCTR*CAA
Frame 1
HQGQWPPVKWTRLRMAYVSFLYLSCCPGILLSHSTFSNKIL*GTSVQKTE*LSCHELKDPLVGVSE
ELSVDLLSAVENYCLLTDSLSILSLKLAPKPLPL
PATADITCVCKLNTFYCKNKSHGCSEQL*FSLG
CA*WRQRTRWFSM*PFPTAEPCLHLMPQKVQPV
CEPHMADSWGPQPGQGVCVQNKQGQGPALAWLSR
ALLPPTSHPAAFIPQALKYAFQTHDRLCFVMEY
ANGGELFFHLSRERVFTEERARFYGAEIVSALE
YLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCK
EGISDGATMKTFCGTPEYLAPEVSGVGGGAGGQ
*LWPGRGLVGAGCGHVRDDVRP
Frame 2
IKASGHQLNGLD*GWLMYHFFISVAVQVSY*ATVLFQIKSYEVPQCRKQNS*VVMS*RTPWWESLR
NSL*ISLVQWRTTVC*LTACPSCH*SWLPSLCH
SQPQQTSLVCVS*THFTVKTRAMVAQNSSSSPLA
VPDGGRGQDGSACNPFLPQSHAFT*CPKRCSRY
VNPIWLTPGGPSQGRVCVCRISRARALPWHGSA
EPSSLPPPTLLPSSPRR*SMPSRPTTACAL*WSM
PTGVSCSSTCPGSVSSQRSGPGFMVQRLSRLLS
TCTRGTWYTATSSWKTSCWTKMATSRSLTLASA
KRASVTGPP*KPSVGPRSTWRLRCLGLVGVLEDN
DYGRAVDWWGLGVVMYEMMCGR
Sequence AKT2.eAug05
GGGGAGGGGGCGGGGGGGGCCGCGCCGTGCTAGCCGTTGGGCCTGCCTCGGAGGAGGCGTCGCCGCCGCCGCTGCCGCTGCCGGCGCCGTTGCCGCTGCCGGGAAACACAAGGAAAGGGAACCAGCGCAGCGTGGCGATGGGCGGGGGTAGAGCCCCGCCGGAGAGGCTGGGCGGCTGCCGGTGACAGGTGAATACATCA
AGACCTGGAGGCCACGGTACTTCCTGCTGAAGAGCGACGGCTCCTTCATTGGGTACAAGGAGAGGCCCGAGGCCCCTGATCAGACTCTACCCCCCTTAAA
CAACTTCTCCGTAGCAGAATGCCAGCTGATGAAGACCGAGAGGCCGCGACCCAACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGG
ACCTTCCACGTGGATTCTCCAGACGAGAGGGAGGAGTGGATGCGGGCCATCCAGATGGTCGCCAACAGCCTCAAGCAGCGGGCCCCAGGCGAGGACCCCA
TGGACTACAAGTGTGGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTCAGCAAGGCACGGGCTAAAGTGACCATGAATGACTTCGA
CTATCTCAAACTCCTTGGCAAGGGAACCTTTGGCAAAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTACGCCATGAAGATCCTGCGGAAGGAA
GTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAGAGCCGGGTCCTCCAGAACACCAGGCACCCGTTCCTCACTGCGCTGAAGTATGCCTTCC
AGACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTCTTCACAGAGGAGCGGGCCCG
GTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAAACCTCATGCTGGACAAAGAT
GGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTG
AGGTGTCTGGGGTTGGTGGGGGTAGGAACGTGTGAGGCCAAGGGTAGGGGGATCCCCCAGACCTGTGTGTTTCCAGCCCAGATGCCCTGAGGGCAGGACT
GTGATGCCAGCCTCAGGGCCACGGGCCCAGCCCTCATTTCTCCTCCATCCTCAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGC
TGGGTGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAGAGATCCGCTT
CCCGCGCACGCTCAGCCCCGAGGCCAAGTCCCTGCTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCCAAGGAG
GTCATGGAGCACAGGTTCTTCCTCAGCATCAACTGGCAGGACGTGGTCCAGAAGAAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACA
CAAGGTACTTCGATGATGAATTTACCGCCCAGTCCATCACAATCACACCCCCTGACCGCTATGACAGCCTGGGCTTACTGGAGCTGGACCAGCGGACCCA
CTTCCCCCAGTTCTCCTACTCGGCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATCACCGCTGGGTGGTTTTTTCCC
CCTAACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCCCCACCTCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGG
CCCCTGCAGCCCCTGCCTCCAGCCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGGGCTGTTGGGCGGTT
GGAGATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCTGGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGC
CTCCGCGCTGCTGCATCTGGACGAAGGCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGGCTCGGAG
TGGCCAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAATAATGTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCA
GGGCTGGGGGTTAGGGCTGCTCCCTGGTGAATGGAGTCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTC
CGGCCTCCTCCCCACCAAACTGTCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCTCCCAGCCCCGTGCCTTACTAGGGC
TTCCTTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGGCCTGGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGG
GCCACCAGCCTTGGCTGTTACAATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGGTGGGCATCTCTGGTACCTATTGGG
GTGGGGGGCCTCTGAAAAGGGAGGCTCCTAGGCCCCCCTCACCCCTCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACG
TCCATGTCAGTGCTGTGGGACTCCTGTGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGTGTTTGTGTGAGG
GCAGCGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTGGGCCCCATCCCTGGCCCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAG
CCCCACTGTGATCCATGAGCTGCCCTGCCCACGCCTCCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTGCTCTCCCTAT
CCCATCTCCTGTACTACACAGTTCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTCCTCTGTGTGCGATGTTGTTATCTG
ACAGTTCTCCGTCCCTACTGGCCTTTCTCCTCGTCTTCATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGG
- AKT2.F10 (forward primer)
- AKT2.R10 (reverse primer)
- Intron retention
Frame 0
GEGAGGAAPC*PLGLPRRRRRRRRCRCRRRCRCRETQGKGTSAAWRWAGVEPRRRGWAAAGDR*IHQ
DLEATVLPAEERRLLHWVQGEARGP*SDSTPLK
QLLRSRMPADEDREAATQHLCHTLPAVDHSHRE
DLPRGFSRREGGVDAGHPDGRQQPQAAGPRRGPH
GLQVWLPQ*LLHD*GDGSGGQQGTG*SDHE*LR
LSQTPWQGNLWQSHPGAGEGHWPLLRHEDPAEG
SHHCQG*SRSHSHREPGPPEHQAPVPHCAEVCLP
DPRPPVLCDGVCQRG*AVLPPVPGACLHRGAGP
VLWCRDCLGS*VLALAGRGIPRHQAGKPHAGQR
WPHQDH*LWPLQRGHQ*RGHHENLLWDPGVPGA*
GVWGWWG*ERVRPRVGGSPRPVCFQPRCPEGRT
VMPASGPRAQPSFLLHPQVLEDNDYGRAVDWWG
LGVVMYEMMCGRLPFYNQDHERLFELILMEEIRF
PRTLSPEAKSLLAGLLKKDPKQRLGGGPSDAKE
VMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVD
TRYFDDEFTAQSITITPPDRYDSLGLLELDQRTH
FPQFSYSASIRE*AVCPRRGRTLAAITAGWFFP
PNFLLSLFGLCPHPHLLTPFPVLLQAPPRRTPA
APAAPASSLQPHLCAQTRIWKTPPPAQAWAVGRL
EIQVLIHTSPSEG*SMAPGACLSFWPGCRAGVC
LRAAASGRRLPSGGTRHPADSGAAFQAPWPRLG
VARHGAVQSPTRCPPMGAEKQ*CPGAGRGPWELQ
GWGLGLLPGEWSQILGSVPWGTRSGRAGCRLLV
RPPPHQTVLTLWMRQEEHLGPNLPASQPRALLG
LPSSWPYLPLDPGPGLAPLGAMGWAHPLLCGGGG
PPALAVTILHRTVLGPMDLVREGWGWASLVPIG
VGGL*KGRLLGPPHPSLSPGPHVLQP*G*T*VH
VHVSAVGLLCVPRTACVGGTQAHVGVCACVFV*G
QRVLQCAWCVGLGPIPGPSISSCGGGVLT*WEE
PHCDP*AALPTPPLPVATPLGVWSLAFVGLLSL
SHLLYYTVHGRVGRGGVGSGG*GSFSSVCDVVI*
QFSVPTGLSPRLHICTVQAIKTLISDQ
Frame 1
GRGRGGPRRASRWACLGGGVAAAAAAAGAVAAAGKHKEREPAQRGDGRG*SPAGEAGRLPVTGEYI
KTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLN
NFSVAECQLMKTERPRPNTFVIRCLQWTTVIER
TFHVDSPDEREEWMRAIQMVANSLKQRAPGEDP
MDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFD
YLKLLGKGTFGKVILVREKATGRYYAMKILRKE
VIIAKDEVAHTVTESRVLQNTRHPFLTALKYAF
QTHDRLCFVMEYANGGELFFHLSRERVFTEERAR
FYGAEIVSALEYLHSRDVVYRDIKLENLMLDKD
GHIKITDFGLCKEGISDGATMKTFCGTPEYLAP
EVSGVGGGRNV*GQG*GDPPDLCVSSPDALRAGL
*CQPQGHGPSPHFSSILRCWRTMTMAGPWTGGG
WVWSCTR*CAAACPSTTRTTSASSSSSSWKRSA
SRARSAPRPSPCLLGCLRRTPSRGLVGGPAMPRR
SWSTGSSSASTGRTWSRRSSCHPSNLRSRPRST
QGTSMMNLPPSPSQSHPLTAMTAWAYWSWTSGP
TSPSSPTRPASASEQSAHAEDARSLPSPLGGFFP
LTFYLAFLVCVPTPTSSPPFQFFFRPLPDAPQR
PLQPLPPASSLTFVPRLAFGRLHLPPRPGLLGG
WRFRF*STQAPVRGEAWRLGPA*VSGLGVVLVSA
SALLHLDEGCLLVGRDTRQTVVLPSRPRGLGSE
WPGTGRSNPPPAVPLWGQKSNNVQGQAGALGSC
RAGG*GCSLVNGVRS*DLYHGEPGVAGLGAASWS
GLLPTKLSSPYG*GRRNIWGQTCLPPSPVPY*G
FLPAGLTSRWTLGLAWPHWGLWAGLTLSSAGVE
GHQPWLLQSYTGQYWAPWTWSGRGGGGHLWYLLG
WGASEKGGS*APLTPPSPQGPTFCSLKVEHECT
SMSVLWDSCACLGLRVSAGRRHTWVCVHVCLCE
GSVSSSVHGVWAWAPSLARAFHPVGEGC*PSGRS
PTVIHELPCPRLPSL*QHLWVFGV*LLWVCSPY
PISCTTQFMAGWGGVGLVRVGEGLFPLCAMLLS
DSSPSLLAFLLVFIFVRYKQ*RHSFQTR
Frame 2
GGGGGGRAVLAVGPASEEASPPPLPLPAPLPLPGNTRKGNQRSVAMGGGRAPPERLGGCR*QVNTS
RPGGHGTSC*RATAPSLGTRRGPRPLIRLYPP*
TTSP*QNAS**RPRGRDPTPLSYAACSGPQSSRG
PSTWILQTRGRSGCGPSRWSPTASSSGPQARTP
WTTSVAPPVTPPRLRRWKWRSARHGLK*P*MTS
TISNSLAREPLAKSSWCGRRPLAATTP*RSCGRK
SSLPRMKSLTQSPRAGSSRTPGTRSSLR*SMPS
RPTTACAL*WSMPTGVSCSSTCPGSVSSQRSGP
GFMVQRLSRLLSTCTRGTWYTATSSWKTSCWTKM
ATSRSLTLASAKRASVTGPP*KPSVGPRSTWRL
RCLGLVGVGTCEAKGRGIPQTCVFPAQMP*GQD
CDASLRATGPALISPPSSGAGGQ*LWPGRGLVGA
GCGHVRDDVRPPALLQPGPRAPLRAHPHGRDPL
PAHAQPRGQVPACWAA*EGPQAEAWWGAQRCQG
GHGAQVLPQHQLAGRGPEEAPATLQTSGHVRGRH
KVLR**IYRPVHHNHTP*PL*QPGLTGAGPADP
LPPVLLLGQHPRVSSLPTQRTHARCHHRWVVFS
P*LFT*PFWFVSPPPPPHPLSSSSSGPSQTHPSG
PCSPCLQPPASPLCPDSHLEDSTSRPGLGCWAV
GDSGFNPHKPQ*GVKHGAWGLPEFLAWVSCWCL
PPRCCIWTKAAFWWDATPGRQWCCLPGPVA*ARS
GQARGGPIPHPLSPYGGRKAIMSRGRQGPLGAA
GLGVRAAPW*MESDPRICTMGNQEWPGWVPPPG
PASSPPNCPHPMDEAGGTFGAKPACLPAPCLTRA
SFQLALPPAGPWAWPGPTGGYGLGSPSPLRGWR
ATSLGCYNLTPDSIGPHGLGQGGVGVGISGTYW
GGGPLKREAPRPPSPLPLPRAPRSAALRLNMSAR
PCQCCGTPVRASDCVCRRDAGTRGCVCMCVCVR
AACPPVCMVCGLGPHPWPEHFILWGRGADLVGG
APL*SMSCPAHASPPCSNTSGCLEFSFCGFALPI
PSPVLHSSWQGGEGWGWFGWVRVFFLCVRCCYL
TVLRPYWPFSSSSYLYGTSNKDTHFRP
Sequence AKT2.shed3.aAug05
GGTGGGCCAGAGGGTGAGGAGGGTTGGATCTGGCCTCTCTCTGAGCCTCAGAGGGTGAGGCTGTGTGTGCTGGGCCCAGCCTGGCATGGGAGGGTTGATGTCCAGGAGCCCCGGGCATCTTTCCCTGGAAGGAAAGGCTGGGGTGGCAGGAAGCCTTGGGAGCATGCACCTGAGATCCGTTGACTTTTCCAACAGCTGGA
AAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGG
ACCCCGGAGTACCTGGCGCCTGAGGTGTCTGGGGTTGGTGGGGGTAGGAACGTGTGAGGCCAAGGGTAGGGGGATCCCCCAGACCTGTGTGTTTCCAGCC
CAGATGCCCTGAGGGCAGGACTGTGATGCCAGCCTCAGGGCCACGGGCCCAGCCCTCATTTCTCCTCCATCCTCAGGTGCTGGAGGACAATGACTATGGC
CGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGC
- AKT2.F10 (forward primer)
- AKT2.R10 (reverse primer)
- Intron retention
Frame 0
GGPEGEEGWIWPLSEPQRVRLCVLGPAWHGRVDVQEPRASFPGRKGWGGRKPWEHAPEIR*LFQQLE
NLMLDKDGHIKITDFGLCKEGISDGATMKTFCG
TPEYLAPEVSGVGGGRNV*GQG*GDPPDLCVSS
PDALRAGL*CQPQGHGPSPHFSSILRCWRTMTMA
GPWTGGGWVWSCTR*CAA
Frame 1
VGQRVRRVGSGLSLSLRG*GCVCWAQPGMGGLMSRSPGHLSLEGKAGVAGSLGSMHLRSVDFSNSW
KTSCWTKMATSRSLTLASAKRASVTGPP*KPSVG
PRSTWRLRCLGLVGVGTCEAKGRGIPQTCVFPA
QMP*GQDCDASLRATGPALISPPSSGAGGQ*LW
PGRGLVGAGCGHVRDDVRP
Frame 2
WARG*GGLDLASL*ASEGEAVCAGPSLAWEG*CPGAPGIFPWKERLGWQEALGACT*DPLTFPTAG
KPHAGQRWPHQDH*LWPLQRGHQ*RGHHENLLW
DPGVPGA*GVWGWWG*ERVRPRVGGSPRPVCFQP
RCPEGRTVMPASGPRAQPSFLLHPQVLEDNDYG
RAVDWWGLGVVMYEMMCGR
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