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Sequences

Sequence AKT2.aAug05

GGGGAGGGGGCGGGGGGGGCCGCGCCGTGCTAGCCGTTGGGCCTGCCTCGGAGGAGGCGTCGCCGCCGCCGCTGCCGCTGCCGGCGCCGTTGCCGCTGCC GGGAAACACAAGGAAAGGGAACCAGCGCAGCGTGGCGATGGGCGGGGGTAGAGCCCCGCCGGAGAGGCTGGGCGGCTGCCGGTGACAGACTGTGCCCTGT CCACGGTGCCTCCTGCATGTCCTGCTGCCCTGAGCTGTCCCGAGCTAGGTGACAGCGTACCACGCTGCCACCATGAATGAGGTGTCTGTCATCAAAGAAG GCTGGCTCCACAAGCGTGGTGAATACATCAAGACCTGGAGGCCACGGTACTTCCTGCTGAAGAGCGACGGCTCCTTCATTGGGTACAAGGAGAGGCCCGA GGCCCCTGATCAGACTCTACCCCCCTTAAACAACTTCTCCGTAGCAGAATGCCAGCTGATGAAGACCGAGAGGCCGCGACCCAACACCTTTGTCATACGC TGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCACGTGGATTCTCCAGACGAGAGGGAGGAGTGGATGCGGGCCATCCAGATGGTCGCCAACAGCC TCAAGCAGCGGGCCCCAGGCGAGGACCCCATGGACTACAAGTGTGGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTCAGCAAGGC ACGGGCTAAAGTGACCATGAATGACTTCGACTATCTCAAACTCCTTGGCAAGGGAACCTTTGGCAAAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGC TACTACGCCATGAAGATCCTGCGGAAGGAAGTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAGAGCCGGGTCCTCCAGAACACCAGGCACC CGTTCCTCACTGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCG GGAGCGTGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATC AAGCTGGAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCT TCTGTGGGACCCCGGAGTACCTGGCGCCTGAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGAT GATGTGCGGCCGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAGAGATCCGCTTCCCGCGCACGCTCAGCCCCGAG GCCAAGTCCCTGCTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCCAAGGAGGTCATGGAGCACAGGTTCTTCC TCAGCATCAACTGGCAGGACGTGGTCCAGAAGAAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACACAAGGTACTTCGATGATGAATT TACCGCCCAGTCCATCACAATCACACCCCCTGACCGCTATGACAGCCTGGGCTTACTGGAGCTGGACCAGCGGACCCACTTCCCCCAGTTCTCCTACTCG GCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATCACCGCTGGGTGGTTTTTTCCCCCTAACTTTTTACTTAGCCTTT TTGGTTTGTGTCCCCACCCCCACCTCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGGCCCCTGCAGCCCCTGCCTCCAG CCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGGGCTGTTGGGCGGTTGGAGATTCAGGTTTTAATCCAC ACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCTGGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGCCTCCGCGCTGCTGCATCTGGAC GAAGGCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGGCTCGGAGTGGCCAGGCACGGGGCGGTCCA ATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAATAATGTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCAGGGCTGGGGGTTAGGGCTGCTC CCTGGTGAATGGAGTCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTCCGGCCTCCTCCCCACCAAACTG TCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCTCCCAGCCCCGTGCCTTACTAGGGCTTCCTTCCAGCTGGCCTTACCT CCCGCTGGACCCTGGGCCTGGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGGGCCACCAGCCTTGGCTGTTACA ATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGGTGGGCATCTCTGGTACCTATTGGGGTGGGGGGCCTCTGAAAAGGGA GGCTCCTAGGCCCCCCTCACCCCTCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACGTCCATGTCAGTGCTGTGGGACT CCTGTGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGTGTTTGTGTGAGGGCAGCGTGTCCTCCAGTGTGCA TGGTGTGTGGGCTTGGGCCCCATCCCTGGCCCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAGCCCCACTGTGATCCATGAGCTG CCCTGCCCACGCCTCCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTGCTCTCCCTATCCCATCTCCTGTACTACACAGT TCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTCCTCTGTGTGCGATGTTGTTATCTGACAGTTCTCCGTCCCTACTGGC CTTTCTCCTCGTCTTCATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGG

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

GEGAGGAAPC*PLGLPRRRRRRRRCRCRRRCRC RETQGKGTSAAWRWAGVEPRRRGWAAAGDRLCPV HGASCMSCCPELSRAR*QRTTLPP*MRCLSSKK AGSTSVVNTSRPGGHGTSC*RATAPSLGTRRGP RPLIRLYPP*TTSP*QNAS**RPRGRDPTPLSYA ACSGPQSSRGPSTWILQTRGRSGCGPSRWSPTA SSSGPQARTPWTTSVAPPVTPPRLRRWKWRSAR HGLK*P*MTSTISNSLAREPLAKSSWCGRRPLAA TTP*RSCGRKSSLPRMKSLTQSPRAGSSRTPGT RSSLR*SMPSRPTTACAL*WSMPTGVSCSSTCP GSVSSQRSGPGFMVQRLSRLLSTCTRGTWYTATS SWKTSCWTKMATSRSLTLASAKRASVTGPP*KP SVGPRSTWRLRCWRTMTMAGPWTGGGWVWSCTR *CAAACPSTTRTTSASSSSSSWKRSASRARSAPR PSPCLLGCLRRTPSRGLVGGPAMPRRSWSTGSS SASTGRTWSRRSSCHPSNLRSRPRSTQGTSMMN LPPSPSQSHPLTAMTAWAYWSWTSGPTSPSSPTR PASASEQSAHAEDARSLPSPLGGFFPLTFYLAF LVCVPTPTSSPPFQFFFRPLPDAPQRPLQPLPP ASSLTFVPRLAFGRLHLPPRPGLLGGWRFRF*ST QAPVRGEAWRLGPA*VSGLGVVLVSASALLHLD EGCLLVGRDTRQTVVLPSRPRGLGSEWPGTGRS NPPPAVPLWGQKSNNVQGQAGALGSCRAGG*GCS LVNGVRS*DLYHGEPGVAGLGAASWSGLLPTKL SSPYG*GRRNIWGQTCLPPSPVPY*GFLPAGLT SRWTLGLAWPHWGLWAGLTLSSAGVEGHQPWLLQ SYTGQYWAPWTWSGRGGGGHLWYLLGWGASEKG GS*APLTPPSPQGPTFCSLKVEHECTSMSVLWD SCACLGLRVSAGRRHTWVCVHVCLCEGSVSSSVH GVWAWAPSLARAFHPVGEGC*PSGRSPTVIHEL PCPRLPSL*QHLWVFGV*LLWVCSPYPISCTTQ FMAGWGGVGLVRVGEGLFPLCAMLLSDSSPSLLA FLLVFIFVRYKQ*RHSFQTR

Frame 1

GRGRGGPRRASRWACLGGGVAAAAAAAGAVAAA GKHKEREPAQRGDGRG*SPAGEAGRLPVTDCAL STVPPACPAALSCPELGDSVPRCHHE*GVCHQRR LAPQAW*IHQDLEATVLPAEERRLLHWVQGEAR GP*SDSTPLKQLLRSRMPADEDREAATQHLCHT LPAVDHSHREDLPRGFSRREGGVDAGHPDGRQQP QAAGPRRGPHGLQVWLPQ*LLHD*GDGSGGQQG TG*SDHE*LRLSQTPWQGNLWQSHPGAGEGHWP LLRHEDPAEGSHHCQG*SRSHSHREPGPPEHQAP VPHCAEVCLPDPRPPVLCDGVCQRG*AVLPPVP GACLHRGAGPVLWCRDCLGS*VLALAGRGIPRH QAGKPHAGQRWPHQDH*LWPLQRGHQ*RGHHENL LWDPGVPGA*GAGGQ*LWPGRGLVGAGCGHVRD DVRPPALLQPGPRAPLRAHPHGRDPLPAHAQPR GQVPACWAA*EGPQAEAWWGAQRCQGGHGAQVLP QHQLAGRGPEEAPATLQTSGHVRGRHKVLR**I YRPVHHNHTP*PL*QPGLTGAGPADPLPPVLLL GQHPRVSSLPTQRTHARCHHRWVVFSP*LFT*PF WFVSPPPPPHPLSSSSSGPSQTHPSGPCSPCLQ PPASPLCPDSHLEDSTSRPGLGCWAVGDSGFNP HKPQ*GVKHGAWGLPEFLAWVSCWCLPPRCCIWT KAAFWWDATPGRQWCCLPGPVA*ARSGQARGGP IPHPLSPYGGRKAIMSRGRQGPLGAAGLGVRAA PW*MESDPRICTMGNQEWPGWVPPPGPASSPPNC PHPMDEAGGTFGAKPACLPAPCLTRASFQLALP PAGPWAWPGPTGGYGLGSPSPLRGWRATSLGCY NLTPDSIGPHGLGQGGVGVGISGTYWGGGPLKRE APRPPSPLPLPRAPRSAALRLNMSARPCQCCGT PVRASDCVCRRDAGTRGCVCMCVCVRAACPPVC MVCGLGPHPWPEHFILWGRGADLVGGAPL*SMSC PAHASPPCSNTSGCLEFSFCGFALPIPSPVLHS SWQGGEGWGWFGWVRVFFLCVRCCYLTVLRPYW PFSSSSYLYGTSNKDTHFRP

Frame 2

GGGGGGRAVLAVGPASEEASPPPLPLPAPLPLP GNTRKGNQRSVAMGGGRAPPERLGGCR*QTVPC PRCLLHVLLP*AVPS*VTAYHAATMNEVSVIKE GWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPE APDQTLPPLNNFSVAECQLMKTERPRPNTFVIR CLQWTTVIERTFHVDSPDEREEWMRAIQMVANS LKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKA RAKVTMNDFDYLKLLGKGTFGKVILVREKATGR YYAMKILRKEVIIAKDEVAHTVTESRVLQNTRH PFLTALKYAFQTHDRLCFVMEYANGGELFFHLSR ERVFTEERARFYGAEIVSALEYLHSRDVVYRDI KLENLMLDKDGHIKITDFGLCKEGISDGATMKT FCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEM MCGRLPFYNQDHERLFELILMEEIRFPRTLSPE AKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFF LSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEF TAQSITITPPDRYDSLGLLELDQRTHFPQFSYS ASIRE*AVCPRRGRTLAAITAGWFFPPNFLLSL FGLCPHPHLLTPFPVLLQAPPRRTPAAPAAPASS LQPHLCAQTRIWKTPPPAQAWAVGRLEIQVLIH TSPSEG*SMAPGACLSFWPGCRAGVCLRAAASG RRLPSGGTRHPADSGAAFQAPWPRLGVARHGAVQ SPTRCPPMGAEKQ*CPGAGRGPWELQGWGLGLL PGEWSQILGSVPWGTRSGRAGCRLLVRPPPHQT VLTLWMRQEEHLGPNLPASQPRALLGLPSSWPYL PLDPGPGLAPLGAMGWAHPLLCGGGGPPALAVT ILHRTVLGPMDLVREGWGWASLVPIGVGGL*KG RLLGPPHPSLSPGPHVLQP*G*T*VHVHVSAVGL LCVPRTACVGGTQAHVGVCACVFV*GQRVLQCA WCVGLGPIPGPSISSCGGGVLT*WEEPHCDP*A ALPTPPLPVATPLGVWSLAFVGLLSLSHLLYYTV HGRVGRGGVGSGG*GSFSSVCDVVI*QFSVPTG LSPRLHICTVQAIKTLISDQ

Sequence AKT2.bAug05

ATGAGTGACAGAACTAGTTTATTGTTCTTTATTTTATTATTATTATTATTATTATTATTATTATTATTATTTTATTATTATTTTGGGATGGAGTCTCGCA CTGTTGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCCGCCTCCCGGGTTCAAATGATCCTCTTGCCTCAGCCTCCCGAGTAGCTG GGATTACAGGCGCCCGTCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACTGGGTTTTACTATGTTGGCCAGGCTGGTCTCGAACTCCTGACCT CAAGTGATCCTCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCATCGCACCTGGCCTGTTTTTATTTTTTATTTTTGTTTTTTAAAAACT TTTTAAGAGATGGGTCTTGCTTTGTTACCTAGGCTGGTCTTGAACGCTTCGGCTCAAGCACTTGGTCCACCTTGGCCTCCCAAAATGCTGGGATTACAGG TGTGAGCCACAGTGCTTAGCCTCTAGTTAACTGTTCTTAAACCAGGCTCCCTTCTGCTGGGACCTACTTCTTGTCCACCAGCTTCTCTCCTCTACATCCC TGGGCTGCCAGGACCTCTGTCCCTGCCATGCAGACTCCCTGCTCCCAGTGGACTGTCCCCTCCAGGGAGTCCTGCGGGCCAGCTCTTGCCTTGCAGTGTC TTCAGCTCCTTTGGCTAATGAATGCTGCTTCTTACTCTTGGTTTAACTTTACAAAACATTGGTAACTTATAAACTTGAGCTTGTCATTTGTTCCGAAGAA TTCACAGGAGCACTCTCTCTCCCTCATTCAAGGCTCAGGGTGGTGGCTCATTTTCAAGGGGCTGTGAAAAGAGAGGGTCACTCCTTTTTGATCATTTCCC TGCTGCCTGGCTTCAAATGGCCCTCAGGCCAGGTCACTTTAGGGAAATTGGTGATCGCTCTTGGTATTCCACTTTTTTTTTTTTTTTGAGACGGAGTCTC GCACTGTCACCAGGCTGGAGTGCAGTGGTGCGATCTCTGCTCACTGCACCCTCCGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGC TGGGATTAGGCGAATGCCACCCCTGACTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTTGTG ATCCATCCGCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCACTGCGCCTGGCGTGGAATTTCACTCAGGTCCCTTCAGCTCTCTCTGGTTCT GACAGATCTTGCCCAAATTCCCATGGTTATGTGGTCATTTGCTTGCCTGTTGCTGGGGGTGGCTGGGAGGTGGCACTCAGCTGTTCGACTCTGAGGCCCA GGACTCCTGGCTGCTGGGACTCACGTGTGTCCCTGCACAGGGAGGAGTGGATGCGGGCCATCCAGATGGTCGCCAACAGCCTCAAGCAGCGGGCCCCAGG CGAGGACCCCATGGACTACAAGTGTGGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTCAGCAAGGCACGGGCTAAAGTGACCATG AATGACTTCGACTATCTCAAACTCCTTGGCAAGGGAACCTTTGGCAAAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTACGCCATGAAGATCC TGCGGAAGGAAGTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAGAGCCGGGTCCTCCAGAACACCAGGCACCCGTTCCTCACTGCGCTGAA GTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTCTTCACAGAG GAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAAACCTCATGC TGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTA CCTGGCGCCTGAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCC TTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAGAGATCCGCTTCCCGCGCACGCTCAGCCCCGAGGCCAAGTCCCTGCTTGCTG GGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCCAAGGAGGTCATGGAGCACAGGTTCTTCCTCAGCATCAACTGGCAGGA CGTGGTCCAGAAGAAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACACAAGGTACTTCGATGATGAATTTACCGCCCAGTCCATCACA ATCACACCCCCTGACCGCTATGACAGCCTGGGCTTACTGGAGCTGGACCAGCGGACCCACTTCCCCCAGTTCTCCTACTCGGCCAGCATCCGCGAGTGAG CAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATCACCGCTGGGTGGTTTTTTCCCCCTAACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCC CCACCTCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGGCCCCTGCAGCCCCTGCCTCCAGCCTCCAGCCTCACCTTTGT GCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGGGCTGTTGGGCGGTTGGAGATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGT GAAGCATGGCGCCTGGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGCCTCCGCGCTGCTGCATCTGGACGAAGGCTGCCTTCTGGTGG GACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGGCTCGGAGTGGCCAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCC CCTATGGGGGCAGAAAAGCAATAATGTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCAGGGCTGGGGGTTAGGGCTGCTCCCTGGTGAATGGAGTCAGA TCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTCCGGCCTCCTCCCCACCAAACTGTCCTCACCCTATGGATGAG GCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCTCCCAGCCCCGTGCCTTACTAGGGCTTCCTTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGGCCT GGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGGGCCACCAGCCTTGGCTGTTACAATCTTACACCGGACAGTAT TGGGCCCCTCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACGTCCATGTCAGTGCTGTGGGACTCCTGTGCGTGCCTCG GACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGTGTTTGTGTGAGGGCAGCGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTG GGCCCCATCCCTGGCCCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAGCCCCACTGTGATCCATGAGCTGCCCTGCCCACGCCTC CCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTGCTCTCCCTATCCCATCTCCTGTACTACACAGTTCATGGCAGGGTGGG GAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTCCTCTGTGTGCGATGTTGTTATCTGACAGTTCTCCGTCCCTACTGGCCTTTCTCCTCGTCTT CATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGGGCCCAGCCTGCACTCACGCCAGCCCAACCACTCTGGGCTTTGCCTTGGTGATGG AGTCAGACCCCTGGGCCCCAGCTCCTCCTGTACTAGCCGTTCCCTTCAGCAAGGAGGGCACTGAGCTCAGGGTGAGGGCAGCTGGGGTGTGTGCAGGAGC TCAGGCTGGAGAGGGTGGGTGGAGCTGGTGCTGTGGGGCTGAGGGGTATGGGAAGGCTCCCCGCATGTGGGGGTGGGGTGGACAGAGACCACTCCAGGCC CTCAGTGCTGCTTAGGCTAAGAGAGGTGGGGTGGAGGGACAGGGCTGGAAGATCTGGGTAGCCCAGAATGAGGAGGGTGCCTGTGCTGTCACTGAATGAG AGGGAGTGGTTCATTCCACCCGGCTGCCGAGCCTCAGAGGGGGGCATTCCTATCCTGCCCCACCTCCCTGTTTATGCTGCCACCTGGAAGCCTTGAGGCC CCCAAATTCCAGTACAGACCCAGTGGTGTGTTCATGGTGGCGTGGTTGCTGTCACCTGGGAGCTCCTGAGCGTTTGGTTAGAACCCTGTTCAGCTTGGGG TCAGCCCTCCCCTAGTCACTGCCCTTTAGCCTGGATGTGTCTGGGCCCCTGCACTTCCCGTGCTTGAGTCACGTGGCTGCATGGCCGGGCGCTGGCCGGA TGGAACACCTCCCCCAGCAAGGGACCAGGGACCAGAGCCCTGGCCTGCCCTGCTGAGCCCTGCTGTGCAGAGGGCCTGGCACAGATGAATTTGAGATTTT GCCGCAAGGTGTTAGCACTTCACACCCATTGAGTCTTTGAGATTTTAAGTGAATGTAAGCAGAAAAAGTCAGATCCAATTTACAGAAATCAGAGTTAGCT ACAGCTAGGACTCGTTTGGTTGGGGTTTTTTAGTTTGTCTTTCTAAAGTCATGTGGACCTTAATTTAATTACAAAAGTCTACCCTGGTGGTCATAAAATA GGCAGGCCTATGAAGAAAGGCCTTTTACTCTTCCATCTCATCCCAGCCCCGAGTTGACCCACGTTGCTGCTCCTCACACCATGGTGATGCAGGTCTCGTA GTGTGGGCACAGGCCTGGCTACCTCATCTTTTTAGTGCCTCTCTCCTCTTCCACAGGATGGGGTCCCACAGCTGCAGCAGCTGGCCCCGTAGTTGAGCAT GTGTGGTTATCCTGTAGAGCTTTTCCCAAGAAGGGTGTTTGAACTTAGAGTCTTAATAAAATCTTACCAAATAAATTTTGAGTAGAATAATCGTCTTTTG CAATGTACATTTTAAAAATTTCACACATTCTTTTTTGTATATAAAGAACAGTGACTGGGCACAGTGGCTCATGCCTGTAATCCCAGCAATTTGGGAGGCC GAGGCGGGCGGGTCTCTTGAGGCCAGGGGTTCGAGACCAGCCTGGGCATCATAGGGAGACCTTCATCTCTACAAAAAATACAAAAATTAGCTGGGCATGG TGGTGCATGCCTGCAATCCCAGCTAACTTGGAAGGCTGAGGTGAGGTGGGAAGATCACTTGAGCCCAGGAGTTTGAGGCTGCAGTGAGCTATGATTGCGG CACTGCACTGCAGCCTGGGACAATGAGACTGTGTCTCTAAAAATAAAAAAAAAAAAAACATGATACATGCTATTAAAAAAGACAGCAAAGCAGGAGTATA AGAAAGGAAATTCACCCGAGGTCGCAGGGCCTTGAGTACTCATTTTGGTGCTGATTACCTCTCTGCAAATGGACACGGCATCATAAATTGGTAGTTTCCT GCTCTTTTTGTGTAATCTTTTCCAGTTAATGTGAAGCCTCTGGGGGCTGCCCTCGTGCACTGATGGTTGTGTGGAGTCGGGGGCGGCAGTGCGATTCCCT TTTAGCTGCTGCATGGGGGGAACTCAGGCTTTCCAGCTGCTTCCTGGGGTTCCATGGGGTAGACCCCTCAACCGCTTCAGCTGCCCCGTTAACAGGAATT GACTTGGTTTCGTTTGGTGCTACCAGCAGTCCTGTAATAAACTAGCTATCCATCTGTA

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

MSDRTSLLFFILLLLLLLLLLLLFYYYFGMESR TVVQAGVQWRDLGSLQPPPPGFK*SSCLSLPSSW DYRRPSPRPANFLYF**RLGFTMLARLVSNS*P QVILHLGLPKCWDYRREPSHLACFYFLFLFFKN FLRDGSCFVT*AGLERFGSSTWSTLASQNAGITG VSHSA*PLVNCS*TRLPSAGTYFLSTSFSPLHP WAARTSVPAMQTPCSQWTVPSRESCGPALALQC LQLLWLMNAASYSWFNFTKHW*LINLSLSFVPKN SQEHSLSLIQGSGWWLIFKGL*KERVTPF*SFP CCLASNGPQARSL*GNW*SLLVFHFFFFFETES RTVTRLECSGAISAHCTLRLPGSSNSPASAS*VA GIRRMPPLTNFCIFSRDGVSPCWPGWSRTPDLV IHPPQPPKVLGL*A*ATAPGVEFHSGPFSSLWF *QILPKFPWLCGHLLACCWGWLGGGTQLFDSEAQ DSWLLGLTCVPAQGGVDAGHPDGRQQPQAAGPR RGPHGLQVWLPQ*LLHD*GDGSGGQQGTG*SDH E*LRLSQTPWQGNLWQSHPGAGEGHWPLLRHEDP AEGSHHCQG*SRSHSHREPGPPEHQAPVPHCAE VCLPDPRPPVLCDGVCQRG*AVLPPVPGACLHR GAGPVLWCRDCLGS*VLALAGRGIPRHQAGKPHA GQRWPHQDH*LWPLQRGHQ*RGHHENLLWDPGV PGA*GAGGQ*LWPGRGLVGAGCGHVRDDVRPPA LLQPGPRAPLRAHPHGRDPLPAHAQPRGQVPACW AA*EGPQAEAWWGAQRCQGGHGAQVLPQHQLAG RGPEEAPATLQTSGHVRGRHKVLR**IYRPVHH NHTP*PL*QPGLTGAGPADPLPPVLLLGQHPRVS SLPTQRTHARCHHRWVVFSP*LFT*PFWFVSPP PPPHPLSSSSSGPSQTHPSGPCSPCLQPPASPL CPDSHLEDSTSRPGLGCWAVGDSGFNPHKPQ*GV KHGAWGLPEFLAWVSCWCLPPRCCIWTKAAFWW DATPGRQWCCLPGPVA*ARSGQARGGPIPHPLS PYGGRKAIMSRGRQGPLGAAGLGVRAAPW*MESD PRICTMGNQEWPGWVPPPGPASSPPNCPHPMDE AGGTFGAKPACLPAPCLTRASFQLALPPAGPWA WPGPTGGYGLGSPSPLRGWRATSLGCYNLTPDSI GPLPLPRAPRSAALRLNMSARPCQCCGTPVRAS DCVCRRDAGTRGCVCMCVCVRAACPPVCMVCGL GPHPWPEHFILWGRGADLVGGAPL*SMSCPAHAS PPCSNTSGCLEFSFCGFALPIPSPVLHSSWQGG EGWGWFGWVRVFFLCVRCCYLTVLRPYWPFSSS SYLYGTSNKDTHFRPGPSLHSRQPNHSGLCLGDG VRPLGPSSSCTSRSLQQGGH*AQGEGSWGVCRS SGWRGWVELVLWG*GVWEGSPHVGVGWTETTPG PQCCLG*ERWGGGTGLEDLGSPE*GGCLCCH*MR GSGSFHPAAEPQRGAFLSCPTSLFMLPPGSLEA PKFQYRPSGVFMVAWLLSPGSS*AFG*NPVQLG VSPPLVTAL*PGCVWAPALPVLESRGCMAGRWPD GTPPPARDQGPEPWPALLSPAVQRAWHR*I*DF AARC*HFTPIESLRF*VNVSRKSQIQFTEIRVS YS*DSFGWGFLVCLSKVMWTLI*LQKSTLVVIK* AGL*RKAFYSSISSQPRVDPRCCSSHHGDAGLV VWAQAWLPHLFSASLLFHRMGSHSCSSWPRS*A CVVIL*SFSQEGCLNLES**NLTK*ILSRIIVFC NVHFKNFTHSFLYIKNSDWAQWLMPVIPAIWEA EAGGSLEARGSRPAWAS*GDLHLYKKYKN*LGM VVHACNPS*LGRLR*GGKIT*AQEFEAAVSYDCG TALQPGTMRLCL*K*KKKKHDTCY*KRQQSRSI RKEIHPRSQGLEYSFWC*LPLCKWTRHHKLVVS CSFCVIFSS*CEASGGCPRALMVVWSRGRQCDSL LAAAWGELRLSSCFLGFHGVDPSTASAAPLTGI DLVSFGATSSPVIN*LSIC

Frame 1

*VTELVYCSLFYYYYYYYYYYYYFIIILGWSLA LLSRLECSGAISAHCNLRLPGSNDPLASASRVA GITGARHHARLIFCIFSRDWVLLCWPGWSRTPDL K*SSTLASQSAGITGVSHRTWPVFIFYFCFLKT F*EMGLALLPRLVLNASAQALGPPWPPKMLGLQ V*ATVLSL*LTVLKPGSLLLGPTSCPPASLLYIP GLPGPLSLPCRLPAPSGLSPPGSPAGQLLPCSV FSSFG**MLLLTLGLTLQNIGNL*T*ACHLFRR IHRSTLSPSFKAQGGGSFSRGCEKRGSLLFDHFP AAWLQMALRPGHFREIGDRSWYSTFFFFLRRSL ALSPGWSAVVRSLLTAPSASRVQAILLPQPPE* LGLGECHP*LIFVFLVETGFHHVGQAGLELLTL* SIRLSLPKCWDYRHEPLRLAWNFTQVPSALSGS DRSCPNSHGYVVICLPVAGGGWEVALSCSTLRP RTPGCWDSRVSLHREEWMRAIQMVANSLKQRAPG EDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTM NDFDYLKLLGKGTFGKVILVREKATGRYYAMKI LRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALK YAFQTHDRLCFVMEYANGGELFFHLSRERVFTE ERARFYGAEIVSALEYLHSRDVVYRDIKLENLM LDKDGHIKITDFGLCKEGISDGATMKTFCGTPEY LAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLP FYNQDHERLFELILMEEIRFPRTLSPEAKSLLA GLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQD VVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSIT ITPPDRYDSLGLLELDQRTHFPQFSYSASIRE* AVCPRRGRTLAAITAGWFFPPNFLLSLFGLCPHP HLLTPFPVLLQAPPRRTPAAPAAPASSLQPHLC AQTRIWKTPPPAQAWAVGRLEIQVLIHTSPSEG *SMAPGACLSFWPGCRAGVCLRAAASGRRLPSGG TRHPADSGAAFQAPWPRLGVARHGAVQSPTRCP PMGAEKQ*CPGAGRGPWELQGWGLGLLPGEWSQ ILGSVPWGTRSGRAGCRLLVRPPPHQTVLTLWMR QEEHLGPNLPASQPRALLGLPSSWPYLPLDPGP GLAPLGAMGWAHPLLCGGGGPPALAVTILHRTV LGPSLSPGPHVLQP*G*T*VHVHVSAVGLLCVPR TACVGGTQAHVGVCACVFV*GQRVLQCAWCVGL GPIPGPSISSCGGGVLT*WEEPHCDP*AALPTP PLPVATPLGVWSLAFVGLLSLSHLLYYTVHGRVG RGGVGSGG*GSFSSVCDVVI*QFSVPTGLSPRL HICTVQAIKTLISDQGPACTHASPTTLGFALVM ESDPWAPAPPVLAVPFSKEGTELRVRAAGVCAGA QAGEGGWSWCCGAEGYGKAPRMWGWGGQRPLQA LSAA*AKRGGVEGQGWKIWVAQNEEGACAVTE* EGVVHSTRLPSLRGGHSYPAPPPCLCCHLEALRP PNSSTDPVVCSWWRGCCHLGAPERLVRTLFSLG SALP*SLPFSLDVSGPLHFPCLSHVAAWPGAGR MEHLPQQGTRDQSPGLPC*ALLCRGPGTDEFEIL PQGVSTSHPLSL*DFK*M*AEKVRSNLQKSELA TARTRLVGVF*FVFLKSCGP*FNYKSLPWWS*N RQAYEERPFTLPSHPSPELTHVAAPHTMVMQVS* CGHRPGYLIFLVPLSSSTGWGPTAAAAGPVVEH VWLSCRAFPKKGV*T*SLNKILPNKF*VE*SSF AMYILKISHILFCI*RTVTGHSGSCL*SQQFGRP RRAGLLRPGVRDQPGHHRETFISTKNTKISWAW WCMPAIPANLEG*GEVGRSLEPRSLRLQ*AMIA ALHCSLGQ*DCVSKNKKKKNMIHAIKKDSKAGV* ERKFTRGRRALSTHFGADYLSANGHGIINW*FP ALFV*SFPVNVKPLGAALVH*WLCGVGGGSAIP F*LLHGGNSGFPAASWGSMG*TPQPLQLPR*QEL TWFRLVLPAVL**TSYPSV

Frame 2

E*QN*FIVLYFIIIIIIIIIIIILLLFWDGVSH CCPGWSAVARSRLTATSASRVQMILLPQPPE*L GLQAPVTTPG*FFVFLVETGFYYVGQAGLELLT SSDPPPWPPKVLGLQA*AIAPGLFLFFIFVF*KL FKRWVLLCYLGWS*TLRLKHLVHLGLPKCWDYR CEPQCLASS*LFLNQAPFCWDLLLVHQLLSSTS LGCQDLCPCHADSLLPVDCPLQGVLRASSCLAVS SAPLANECCFLLLV*LYKTLVTYKLELVICSEE FTGALSLPHSRLRVVAHFQGAVKREGHSFLIIS LLPGFKWPSGQVTLGKLVIALGIPLFFFF*DGVS HCHQAGVQWCDLCSLHPPPPGFKQFSCLSLLSS WD*ANATPD*FLYF**RRGFTMLARLVSNS*PC DPSASASQSAGIIGMSHCAWRGISLRSLQLSLVL TDLAQIPMVMWSFACLLLGVAGRWHSAVRL*GP GLLAAGTHVCPCTGRSGCGPSRWSPTASSSGPQ ARTPWTTSVAPPVTPPRLRRWKWRSARHGLK*P* MTSTISNSLAREPLAKSSWCGRRPLAATTP*RS CGRKSSLPRMKSLTQSPRAGSSRTPGTRSSLR* SMPSRPTTACAL*WSMPTGVSCSSTCPGSVSSQR SGPGFMVQRLSRLLSTCTRGTWYTATSSWKTSC WTKMATSRSLTLASAKRASVTGPP*KPSVGPRS TWRLRCWRTMTMAGPWTGGGWVWSCTR*CAAACP STTRTTSASSSSSSWKRSASRARSAPRPSPCLL GCLRRTPSRGLVGGPAMPRRSWSTGSSSASTGR TWSRRSSCHPSNLRSRPRSTQGTSMMNLPPSPSQ SHPLTAMTAWAYWSWTSGPTSPSSPTRPASASE QSAHAEDARSLPSPLGGFFPLTFYLAFLVCVPT PTSSPPFQFFFRPLPDAPQRPLQPLPPASSLTFV PRLAFGRLHLPPRPGLLGGWRFRF*STQAPVRG EAWRLGPA*VSGLGVVLVSASALLHLDEGCLLV GRDTRQTVVLPSRPRGLGSEWPGTGRSNPPPAVP LWGQKSNNVQGQAGALGSCRAGG*GCSLVNGVR S*DLYHGEPGVAGLGAASWSGLLPTKLSSPYG* GRRNIWGQTCLPPSPVPY*GFLPAGLTSRWTLGL AWPHWGLWAGLTLSSAGVEGHQPWLLQSYTGQY WAPPSPQGPTFCSLKVEHECTSMSVLWDSCACL GLRVSAGRRHTWVCVHVCLCEGSVSSSVHGVWAW APSLARAFHPVGEGC*PSGRSPTVIHELPCPRL PSL*QHLWVFGV*LLWVCSPYPISCTTQFMAGW GGVGLVRVGEGLFPLCAMLLSDSSPSLLAFLLVF IFVRYKQ*RHSFQTRAQPALTPAQPLWALPW*W SQTPGPQLLLY*PFPSARRALSSG*GQLGCVQE LRLERVGGAGAVGLRGMGRLPACGGGVDRDHSRP SVLLRLREVGWRDRAGRSG*PRMRRVPVLSLNE REWFIPPGCRASEGGIPILPHLPVYAATWKP*G PQIPVQTQWCVHGGVVAVTWELLSVWLEPCSAWG QPSPSHCPLAWMCLGPCTSRA*VTWLHGRALAG WNTSPSKGPGTRALACPAEPCCAEGLAQMNLRF CRKVLALHTH*VFEILSECKQKKSDPIYRNQS*L QLGLVWLGFFSLSF*SHVDLNLITKVYPGGHKI GRPMKKGLLLFHLIPAPS*PTLLLLTPW*CRSR SVGTGLATSSF*CLSPLPQDGVPQLQQLAP*LSM CGYPVELFPRRVFELRVLIKSYQINFE*NNRLL QCTF*KFHTFFFVYKEQ*LGTVAHACNPSNLGG RGGRVS*GQGFETSLGIIGRPSSLQKIQKLAGHG GACLQSQLTWKAEVRWEDHLSPGV*GCSEL*LR HCTAAWDNETVSLKIKKKKT*YMLLKKTAKQEY KKGNSPEVAGP*VLILVLITSLQMDTAS*IGSFL LFLCNLFQLM*SLWGLPSCTDGCVESGAAVRFP FSCCMGGTQAFQLLPGVPWGRPLNRFSCPVNRN *LGFVWCYQQSCNKLAIHL

Sequence AKT2.cAug05

GTGCCCGGCGGGGGGACCGCGGCCCTCGAGCCCCTCCTTCAGGAACTGTGCCCTGTCCACGGTGCCTCCTGCATGTCCTGCTGCCCTGAGCTGTCCCGAG CTAGGTGACAGCGTACCACGCTGCCACCATGAATGAGGTGTCTGTCATCAAAGAAGGCTGGCTCCACAAGCGTGGTGAATACATCAAGACCTGGAGGCCA CGGTACTTCCTGCTGAAGAGCGACGGCTCCTTCATTGGGTACAAGGAGAGGCCCGAGGCCCCTGATCAGACTCTACCCCCCTTAAACAACTTCTCCGTAG CAGAATGCCAGCTGATGAAGACCGAGAGGCCGCGACCCAACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCACGTGGA TTCTCCAGACGAGAGGGAGGAGTGGATGCGGGCCATCCAGATGGTCGCCAACAGCCTCAAGCAGCGGGCCCCAGGCGAGGACCCCATGGACTACAAGTGT GGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTCAGCAAGGCACGGGCTAAAGTGACCATGAATGACTTCGACTATCTCAAACTCC TTGGCAAGGGAACCTTTGGCAAAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTACGCCATGAAGATCCTGCGGAAGGAAGTCATCATTGCCAA GGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTC TTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAA ACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGAC CCCGGAGTACCTGGCGCCTGAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGC CGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAGAGATCCGCTTCCCGCGCACGCTCAGCCCCGAGGCCAAGTCCC TGCTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCCAAGGAGGTCATGGAGCACAGGTTCTTCCTCAGCATCAA CTGGCAGGACGTGGTCCAGAAGAAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACACAAGATGACAGCCTGGGCTTACTGGAGCTGGA CCAGCGGACCCACTTCCCCCAGTTCTCCTACTCGGCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATCACCGCTGGG TGGTTTTTTCCCCCTAACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCCCCACCTCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGAC GCACCCCAGCGGCCCCTGCAGCCCCTGCCTCCAGCCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGGGC TGTTGGGCGGTTGGAGATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCTGGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGT GCTGGTGTCTGCCTCCGCGCTGCTGCATCTGGACGAAGGCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGC CTAGGCTCGGAGTGGCCAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAATAATGTCCAGGGGCAGGCAGGGGCCC TTGGGAGCTGCAGGGCTGGGGGTTAGGGCTGCTCCCTGGTGAATGGAGTCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGC CGCCTCCTGGTCCGGCCTCCTCCCCACCAAACTGTCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCTCCCAGCCCCGTG CCTTACTAGGGCTTCCTTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGGCCTGGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTG CGGGGGTGGAGGGCCACCAGCCTTGGCTGTTACAATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGGTGGGCATCTCTG GTACCTATTGGGGTGGGGGGCCTCTGAAAAGGGAGGCTCCTAGGCCCCCCTCACCCCTCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAA CATGAGTGCACGTCCATGTCAGTGCTGTGGGACTCCTGTGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGT GTTTGTGTGAGGGCAGCGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTGGGCCCCATCCCTGGCCCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACC TAGTGGGAGGAGCCCCACTGTGATCCATGAGCTGCCCTGCCCACGCCTCCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTT TGCTCTCCCTATCCCATCTCCTGTACTACACAGTTCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTCCTCTGTGTGCGA TGTTGTTATCTGACAGTTCTCCGTCCCTACTGGCCTTTCTCCTCGTCTTCATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGG

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

VPGGGTAALEPLLQELCPVHGASCMSCCPELSR AR*QRTTLPP*MRCLSSKKAGSTSVVNTSRPGGH GTSC*RATAPSLGTRRGPRPLIRLYPP*TTSP* QNAS**RPRGRDPTPLSYAACSGPQSSRGPSTW ILQTRGRSGCGPSRWSPTASSSGPQARTPWTTSV APPVTPPRLRRWKWRSARHGLK*P*MTSTISNS LAREPLAKSSWCGRRPLAATTP*RSCGRKSSLP RR*SMPSRPTTACAL*WSMPTGVSCSSTCPGSVS SQRSGPGFMVQRLSRLLSTCTRGTWYTATSSWK TSCWTKMATSRSLTLASAKRASVTGPP*KPSVG PRSTWRLRCWRTMTMAGPWTGGGWVWSCTR*CAA ACPSTTRTTSASSSSSSWKRSASRARSAPRPSP CLLGCLRRTPSRGLVGGPAMPRRSWSTGSSSAS TGRTWSRRSSCHPSNLRSRPRSTQDDSLGLLELD QRTHFPQFSYSASIRE*AVCPRRGRTLAAITAG WFFPPNFLLSLFGLCPHPHLLTPFPVLLQAPPR RTPAAPAAPASSLQPHLCAQTRIWKTPPPAQAWA VGRLEIQVLIHTSPSEG*SMAPGACLSFWPGCR AGVCLRAAASGRRLPSGGTRHPADSGAAFQAPW PRLGVARHGAVQSPTRCPPMGAEKQ*CPGAGRGP WELQGWGLGLLPGEWSQILGSVPWGTRSGRAGC RLLVRPPPHQTVLTLWMRQEEHLGPNLPASQPR ALLGLPSSWPYLPLDPGPGLAPLGAMGWAHPLLC GGGGPPALAVTILHRTVLGPMDLVREGWGWASL VPIGVGGL*KGRLLGPPHPSLSPGPHVLQP*G* T*VHVHVSAVGLLCVPRTACVGGTQAHVGVCACV FV*GQRVLQCAWCVGLGPIPGPSISSCGGGVLT *WEEPHCDP*AALPTPPLPVATPLGVWSLAFVG LLSLSHLLYYTVHGRVGRGGVGSGG*GSFSSVCD VVI*QFSVPTGLSPRLHICTVQAIKTLISDQ

Frame 1

CPAGGPRPSSPSFRNCALSTVPPACPAALSCPE LGDSVPRCHHE*GVCHQRRLAPQAW*IHQDLEA TVLPAEERRLLHWVQGEARGP*SDSTPLKQLLRS RMPADEDREAATQHLCHTLPAVDHSHREDLPRG FSRREGGVDAGHPDGRQQPQAAGPRRGPHGLQV WLPQ*LLHD*GDGSGGQQGTG*SDHE*LRLSQTP WQGNLWQSHPGAGEGHWPLLRHEDPAEGSHHCQ GAEVCLPDPRPPVLCDGVCQRG*AVLPPVPGAC LHRGAGPVLWCRDCLGS*VLALAGRGIPRHQAGK PHAGQRWPHQDH*LWPLQRGHQ*RGHHENLLWD PGVPGA*GAGGQ*LWPGRGLVGAGCGHVRDDVR PPALLQPGPRAPLRAHPHGRDPLPAHAQPRGQVP ACWAA*EGPQAEAWWGAQRCQGGHGAQVLPQHQ LAGRGPEEAPATLQTSGHVRGRHKMTAWAYWSW TSGPTSPSSPTRPASASEQSAHAEDARSLPSPLG GFFPLTFYLAFLVCVPTPTSSPPFQFFFRPLPD APQRPLQPLPPASSLTFVPRLAFGRLHLPPRPG LLGGWRFRF*STQAPVRGEAWRLGPA*VSGLGVV LVSASALLHLDEGCLLVGRDTRQTVVLPSRPRG LGSEWPGTGRSNPPPAVPLWGQKSNNVQGQAGA LGSCRAGG*GCSLVNGVRS*DLYHGEPGVAGLGA ASWSGLLPTKLSSPYG*GRRNIWGQTCLPPSPV PY*GFLPAGLTSRWTLGLAWPHWGLWAGLTLSS AGVEGHQPWLLQSYTGQYWAPWTWSGRGGGGHLW YLLGWGASEKGGS*APLTPPSPQGPTFCSLKVE HECTSMSVLWDSCACLGLRVSAGRRHTWVCVHV CLCEGSVSSSVHGVWAWAPSLARAFHPVGEGC*P SGRSPTVIHELPCPRLPSL*QHLWVFGV*LLWV CSPYPISCTTQFMAGWGGVGLVRVGEGLFPLCA MLLSDSSPSLLAFLLVFIFVRYKQ*RHSFQTR

Frame 2

ARRGDRGPRAPPSGTVPCPRCLLHVLLP*AVPS *VTAYHAATMNEVSVIKEGWLHKRGEYIKTWRP RYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSV AECQLMKTERPRPNTFVIRCLQWTTVIERTFHVD SPDEREEWMRAIQMVANSLKQRAPGEDPMDYKC GSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKL LGKGTFGKVILVREKATGRYYAMKILRKEVIIAK ALKYAFQTHDRLCFVMEYANGGELFFHLSRERV FTEERARFYGAEIVSALEYLHSRDVVYRDIKLE NLMLDKDGHIKITDFGLCKEGISDGATMKTFCGT PEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCG RLPFYNQDHERLFELILMEEIRFPRTLSPEAKS LLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSIN WQDVVQKKLLPPFKPQVTSEVDTR*QPGLTGAG PADPLPPVLLLGQHPRVSSLPTQRTHARCHHRW VVFSP*LFT*PFWFVSPPPPPHPLSSSSSGPSQT HPSGPCSPCLQPPASPLCPDSHLEDSTSRPGLG CWAVGDSGFNPHKPQ*GVKHGAWGLPEFLAWVS CWCLPPRCCIWTKAAFWWDATPGRQWCCLPGPVA *ARSGQARGGPIPHPLSPYGGRKAIMSRGRQGP LGAAGLGVRAAPW*MESDPRICTMGNQEWPGWV PPPGPASSPPNCPHPMDEAGGTFGAKPACLPAPC LTRASFQLALPPAGPWAWPGPTGGYGLGSPSPL RGWRATSLGCYNLTPDSIGPHGLGQGGVGVGIS GTYWGGGPLKREAPRPPSPLPLPRAPRSAALRLN MSARPCQCCGTPVRASDCVCRRDAGTRGCVCMC VCVRAACPPVCMVCGLGPHPWPEHFILWGRGAD LVGGAPL*SMSCPAHASPPCSNTSGCLEFSFCGF ALPIPSPVLHSSWQGGEGWGWFGWVRVFFLCVR CCYLTVLRPYWPFSSSSYLYGTSNKDTHFRP

Sequence AKT2.gAug05

CCATCAAGGCCAGTGGCCACCAGTTAAATGGACTAGATTGAGGATGGCTTATGTATCATTTCTTTATCTCAGTTGCTGTCCAGGTATCCTATTAAGCCAC AGTACTTTTTCAAATAAAATCTTATGAGGTACCTCAGTGCAGAAAACAGAATAGTTGAGTTGTCATGAGCTGAAGGACCCCCTGGTGGGAGTCTCTGAGG AACTCTCTGTAGATCTCCTTAGTGCAGTGGAGAACTACTGTTTGTTGACTGACAGCCTGTCCATCCTGTCATTGAAGTTGGCTCCCAAGCCTTTGCCACT CCCAGCCACAGCAGACATCACTTGTGTGTGTAAGTTGAACACATTTTACTGTAAAAACAAGAGCCATGGTTGCTCAGAACAGCTCTAGTTCTCCCTTGGC TGTGCCTGATGGAGGCAGAGGACAAGATGGTTCAGCATGTAACCCTTTCCTACCGCAGAGCCATGCCTTCACTTAATGCCCCAAAAGGTGCAGCCGGTAT GTGAACCCCATATGGCTGACTCCTGGGGGCCCCAGCCAGGGCAGGGTGTGTGTGTGCAGAATAAGCAGGGCCAGGGCCCTGCCCTGGCATGGCTCAGCAG AGCCCTCCTCCCTCCCACCTCCCACCCTGCTGCCTTCATCCCCCAGGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTAT GCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGT ACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGGTTAGTGGCAGGGTGTGCGCCAGGCTGACCTGTGGCATGCACACCACATGGGACTCACACTG GGGAAGTTGGGCTGAGCTTCTGCCCTCATTCGCCAGGCTCCCCACCAAGGTCCAGGGTGGTGGCTGCCCTCATTCGCCAGGCTCCCCACCAAGGTCCAGG GTGGTGGCTGCCCTCATTCACCAGGCTCCCCACCAAGGTCCAGGGTGGTGGCTCTGTGGCAAGTTGCCCCAAGTAGGGACCTTCCCAAGCCTCCCATGAG CGCTCCTAGGCCACTAGCGGAGTGTCAACTGCCACAATACCTACTTTATTTGTATTTATTTATTTATTTATTTTTGAGGAGTCTTACTCTGTTGAGTGCA GTGGTGTGATCTCGGCTCGCTGCAACCTCTGCCTCCCGGGTTCAAGCGATTGTCCTGCCTCAGCCTCCCGAATAGGTGGGATTACAGGTGCCCGCCACCA TGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCATCATATTGGCCAGGCTGGTCTCAAACTCCTGACCTTGTGATCCACCCACCTCAGCCTC CCAAAGTGCTTGGATTACAGGCATGAGCCCCCGCGCCCGGCCAATTTTAGAAGATAGTTTGGCAGTGTCCACCAAAGCTGAGCACCAGTCATCTGCCCTA GCTGTGTGCACAGGAGAAGTGGGGACACACACTCACCTTAGAAAGTGTCCCCCTCACTCAGCAGCAGGACTTACTCTAGCCCCAACTGGAACAACCCAAG TATCCACTGTGAGAGGAACAGTCAGATCCACTGTGGAGTGTTCTCACAGTGGGACGCCACACAGCAGTTAGGAATGAGCTACAGCTACACACGACAGTGT GGACGCGTCTCATAGGCTAATGTCGAGGGAAAGCCGCACAGAGGAGGTGAGTCCACAGTTCACAGGTGTGTCTGTGTGCAGTGGTACAGATGGGAAGTAG AGGTTAATTCTTGTGGGGGATGGTCAGTAACCGGGAGGGGCACACAGGGCCTTCCAGGGGGAGATGCCACCTCAGCCTCGCAAGTAGCTGGGACTATAGG TGTGTGCCACCATGCCTGGCTAATTTTTTTTTTCATATTAAGAAATATGGAGATGGGGTCTCGCTGTGTTGCCCAGGCTGGTCTCAAACTCATGGGTTCA AGCAATCCTGCCACCTAAGTCCCCCAAATTGCTGGGATTACAGGTGTGAGCCACTTTGCCTGGCTGCATATTTCATAATTAAAGGACAGAAAGTCTAGAA AAAAAGAATAGAAGAAAGCTAGTGCCCCCACGTTCGAAATAAGAACCACAGTTCACAATGGGGACGGTGGGTGGACAGGGCCTGGCTCTCGGCTCTCCCT GCCCAGCTGTTCCCAGGTACCATGGGGGTGGGCCAGAGGGTGAGGAGGGTTGGATCTGGCCTCTCTCTGAGCCTCAGAGGGTGAGGCTGTGTGTGCTGGG CCCAGCCTGGCATGGGAGGGTTGATGTCCAGGAGCCCCGGGCATCTTTCCCTGGAAGGAAAGGCTGGGGTGGCAGGAAGCCTTGGGAGCATGCACCTGAG ATCCGTTGACTTTTCCAACAGCTGGAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACG GGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTGAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGG TGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAGAGATCCGCTTCCCG CGCACGCTCAGCCCCGAGGCCAAGTCCCTGCTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCCAAGGAGGTCA TGGAGCACAGGTTCTTCCTCAGCATCAACTGGCAGGACGTGGTCCAGAAGAAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACACAAG GTACTTCGATGATGAATTTACCGCCCAGTCCATCACAATCACACCCCCTGACCGCTATGACAGCCTGGGCTTACTGGAGCTGGACCAGCGGACCCACTTC CCCCAGTTCTCCTACTCGGCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATCACCGCTGGGTGGTTTTTTCCCCCTA ACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCCCCACCTCCTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGGCCCC TGCAGCCCCTGCCTCCAGCCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGGGCTGTTGGGCGGTTGGAG ATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCTGGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGCCTCC GCGCTGCTGCATCTGGACGAAGGCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGGCTCGGAGTGGC CAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAATAATGTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCAGGGC TGGGGGTTAGGGCTGCTCCCTGGTGAATGGAGTCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTCCGGC CTCCTCCCCACCAAACTGTCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTGCCTGCCTCCCAGCCCCGTGCCTTACTAGGGCTTCC TTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGGCCTGGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGGGCCA CCAGCCTTGGCTGTTACAATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGGTGGGCATCTCTGGTACCTATTGGGGTGG GGGGCCTCTGAAAAGGGAGGCTCCTAGGCCCCCCTCACCCCTCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACGTCCA TGTCAGTGCTGTGGGACTCCTGTGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCATGTGTGTTTGTGTGAGGGCAG CGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTGGGCCCCATCCCTGGCCCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAGCCCC ACTGTGATCCATGAGCTGCCCTGCCCACGCCTCCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTGCTCTCCCTATCCCA TCTCCTGTACTACACAGTTCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGTGGGTGAGGGTCTTTTTCCTCTGTGTGCGATGTTGTTATCTGACAG TTCTCCGTCCCTACTGGCCTTTCTCCTCGTCTTCATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGG

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

PSRPVATS*MD*IEDGLCIISLSQLLSRYPIKP QYFFK*NLMRYLSAENRIVELS*AEGPPGGSL*G TLCRSP*CSGELLFVD*QPVHPVIEVGSQAFAT PSHSRHHLCV*VEHILL*KQEPWLLRTALVLPW LCLMEAEDKMVQHVTLSYRRAMPSLNAPKGAAGM *TPYG*LLGAPARAGCVCAE*AGPGPCPGMAQQ SPPPSHLPPCCLHPPGAEVCLPDPRPPVLCDGV CQRG*AVLPPVPGACLHRGAGPVLWCRDCLGS*V LALAGRGIPRHQG*WQGVRQADLWHAHHMGLTL GKLG*ASALIRQAPHQGPGWWLPSFARLPTKVQ GGGCPHSPGSPPRSRVVALWQVAPSRDLPKPPMS APRPLAECQLPQYLLYLYLFIYLFLRSLTLLSA VV*SRLAATSASRVQAIVLPQPPE*VGLQVPAT MPS*FLYF**RRGFIILARLVSNS*PCDPPTSAS QSAWITGMSPRARPILEDSLAVSTKAEHQSSAL AVCTGEVGTHTHLRKCPPHSAAGLTLAPTGTTQ VSTVRGTVRSTVECSHSGTPHSS*E*ATATHDSV DASHRLMSRESRTEEVSPQFTGVSVCSGTDGK* RLILVGDGQ*PGGAHRAFQGEMPPQPRK*LGL* VCATMPG*FFFSY*EIWRWGLAVLPRLVSNSWVQ AILPPKSPKLLGLQV*ATLPGCIFHN*RTESLE KKNRRKLVPPRSK*EPQFTMGTVGGQGLALGSP CPAVPRYHGGGPEGEEGWIWPLSEPQRVRLCVLG PAWHGRVDVQEPRASFPGRKGWGGRKPWEHAPE IR*LFQQLENLMLDKDGHIKITDFGLCKEGISD GATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLG VVMYEMMCGRLPFYNQDHERLFELILMEEIRFP RTLSPEAKSLLAGLLKKDPKQRLGGGPSDAKEV MEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTR YFDDEFTAQSITITPPDRYDSLGLLELDQRTHF PQFSYSASIRE*AVCPRRGRTLAAITAGWFFPP NFLLSLFGLCPHPHLLTPFPVLLQAPPRRTPAAP AAPASSLQPHLCAQTRIWKTPPPAQAWAVGRLE IQVLIHTSPSEG*SMAPGACLSFWPGCRAGVCL RAAASGRRLPSGGTRHPADSGAAFQAPWPRLGVA RHGAVQSPTRCPPMGAEKQ*CPGAGRGPWELQG WGLGLLPGEWSQILGSVPWGTRSGRAGCRLLVR PPPHQTVLTLWMRQEEHLGPNLPASQPRALLGLP SSWPYLPLDPGPGLAPLGAMGWAHPLLCGGGGP PALAVTILHRTVLGPMDLVREGWGWASLVPIGV GGL*KGRLLGPPHPSLSPGPHVLQP*G*T*VHVH VSAVGLLCVPRTACVGGTQAHVGVCACVFV*GQ RVLQCAWCVGLGPIPGPSISSCGGGVLT*WEEP HCDP*AALPTPPLPVATPLGVWSLAFVGLLSLSH LLYYTVHGRVGRGGVGSGG*GSFSSVCDVVI*Q FSVPTGLSPRLHICTVQAIKTLISDQ

Frame 1

HQGQWPPVKWTRLRMAYVSFLYLSCCPGILLSH STFSNKIL*GTSVQKTE*LSCHELKDPLVGVSE ELSVDLLSAVENYCLLTDSLSILSLKLAPKPLPL PATADITCVCKLNTFYCKNKSHGCSEQL*FSLG CA*WRQRTRWFSM*PFPTAEPCLHLMPQKVQPV CEPHMADSWGPQPGQGVCVQNKQGQGPALAWLSR ALLPPTSHPAAFIPQALKYAFQTHDRLCFVMEY ANGGELFFHLSRERVFTEERARFYGAEIVSALE YLHSRDVVYRDIKVSGRVCARLTCGMHTTWDSHW GSWAELLPSFARLPTKVQGGGCPHSPGSPPRSR VVAALIHQAPHQGPGWWLCGKLPQVGTFPSLP* ALLGH*RSVNCHNTYFICIYLFIYF*GVLLC*VQ WCDLGSLQPLPPGFKRLSCLSLPNRWDYRCPPP CPANFCIFSRDGVSSYWPGWSQTPDLVIHPPQP PKVLGLQA*APAPGQF*KIVWQCPPKLSTSHLP* LCAQEKWGHTLTLESVPLTQQQDLL*PQLEQPK YPL*EEQSDPLWSVLTVGRHTAVRNELQLHTTV WTRLIG*CRGKAAQRR*VHSSQVCLCAVVQMGSR G*FLWGMVSNREGHTGPSRGRCHLSLASSWDYR CVPPCLANFFFHIKKYGDGVSLCCPGWSQTHGF KQSCHLSPPNCWDYRCEPLCLAAYFIIKGQKV*K KRIEES*CPHVRNKNHSSQWGRWVDRAWLSALP AQLFPGTMGVGQRVRRVGSGLSLSLRG*GCVCW AQPGMGGLMSRSPGHLSLEGKAGVAGSLGSMHLR SVDFSNSWKTSCWTKMATSRSLTLASAKRASVT GPP*KPSVGPRSTWRLRCWRTMTMAGPWTGGGW VWSCTR*CAAACPSTTRTTSASSSSSSWKRSASR ARSAPRPSPCLLGCLRRTPSRGLVGGPAMPRRS WSTGSSSASTGRTWSRRSSCHPSNLRSRPRSTQ GTSMMNLPPSPSQSHPLTAMTAWAYWSWTSGPTS PSSPTRPASASEQSAHAEDARSLPSPLGGFFPL TFYLAFLVCVPTPTSSPPFQFFFRPLPDAPQRP LQPLPPASSLTFVPRLAFGRLHLPPRPGLLGGWR FRF*STQAPVRGEAWRLGPA*VSGLGVVLVSAS ALLHLDEGCLLVGRDTRQTVVLPSRPRGLGSEW PGTGRSNPPPAVPLWGQKSNNVQGQAGALGSCRA GG*GCSLVNGVRS*DLYHGEPGVAGLGAASWSG LLPTKLSSPYG*GRRNIWGQTCLPPSPVPY*GF LPAGLTSRWTLGLAWPHWGLWAGLTLSSAGVEGH QPWLLQSYTGQYWAPWTWSGRGGGGHLWYLLGW GASEKGGS*APLTPPSPQGPTFCSLKVEHECTS MSVLWDSCACLGLRVSAGRRHTWVCVHVCLCEGS VSSSVHGVWAWAPSLARAFHPVGEGC*PSGRSP TVIHELPCPRLPSL*QHLWVFGV*LLWVCSPYP ISCTTQFMAGWGGVGLVRVGEGLFPLCAMLLSDS SPSLLAFLLVFIFVRYKQ*RHSFQTR

Frame 2

IKASGHQLNGLD*GWLMYHFFISVAVQVSY*AT VLFQIKSYEVPQCRKQNS*VVMS*RTPWWESLR NSL*ISLVQWRTTVC*LTACPSCH*SWLPSLCH SQPQQTSLVCVS*THFTVKTRAMVAQNSSSSPLA VPDGGRGQDGSACNPFLPQSHAFT*CPKRCSRY VNPIWLTPGGPSQGRVCVCRISRARALPWHGSA EPSSLPPPTLLPSSPRR*SMPSRPTTACAL*WSM PTGVSCSSTCPGSVSSQRSGPGFMVQRLSRLLS TCTRGTWYTATSRLVAGCAPG*PVACTPHGTHT GEVGLSFCPHSPGSPPRSRVVAALIRQAPHQGPG WWLPSFTRLPTKVQGGGSVASCPK*GPSQASHE RS*ATSGVSTATIPTLFVFIYLFIFEESYSVEC SGVISARCNLCLPGSSDCPASASRIGGITGARHH AQLIFVFLVETGFHHIGQAGLKLLTL*STHLSL PKCLDYRHEPPRPANFRR*FGSVHQS*APVICP SCVHRRSGDTHSP*KVSPSLSSRTYSSPNWNNPS IHCERNSQIHCGVFSQWDATQQLGMSYSYTRQC GRVS*ANVEGKPHRGGESTVHRCVCVQWYRWEV EVNSCGGWSVTGRGTQGLPGGDATSASQVAGTIG VCHHAWLIFFFILRNMEMGSRCVAQAGLKLMGS SNPAT*VPQIAGITGVSHFAWLHIS*LKDRKSR KKE*KKASAPTFEIRTTVHNGDGGWTGPGSRLSL PSCSQVPWGWARG*GGLDLASL*ASEGEAVCAG PSLAWEG*CPGAPGIFPWKERLGWQEALGACT* DPLTFPTAGKPHAGQRWPHQDH*LWPLQRGHQ*R GHHENLLWDPGVPGA*GAGGQ*LWPGRGLVGAG CGHVRDDVRPPALLQPGPRAPLRAHPHGRDPLP AHAQPRGQVPACWAA*EGPQAEAWWGAQRCQGGH GAQVLPQHQLAGRGPEEAPATLQTSGHVRGRHK VLR**IYRPVHHNHTP*PL*QPGLTGAGPADPL PPVLLLGQHPRVSSLPTQRTHARCHHRWVVFSP* LFT*PFWFVSPPPPPHPLSSSSSGPSQTHPSGP CSPCLQPPASPLCPDSHLEDSTSRPGLGCWAVG DSGFNPHKPQ*GVKHGAWGLPEFLAWVSCWCLPP RCCIWTKAAFWWDATPGRQWCCLPGPVA*ARSG QARGGPIPHPLSPYGGRKAIMSRGRQGPLGAAG LGVRAAPW*MESDPRICTMGNQEWPGWVPPPGPA SSPPNCPHPMDEAGGTFGAKPACLPAPCLTRAS FQLALPPAGPWAWPGPTGGYGLGSPSPLRGWRA TSLGCYNLTPDSIGPHGLGQGGVGVGISGTYWGG GPLKREAPRPPSPLPLPRAPRSAALRLNMSARP CQCCGTPVRASDCVCRRDAGTRGCVCMCVCVRA ACPPVCMVCGLGPHPWPEHFILWGRGADLVGGAP L*SMSCPAHASPPCSNTSGCLEFSFCGFALPIP SPVLHSSWQGGEGWGWFGWVRVFFLCVRCCYLT VLRPYWPFSSSSYLYGTSNKDTHFRP

Sequence AKT2.hAug05

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

PSRPVATS*MD*IEDGLCIISLSQLLSRYPIKP QYFFK*NLMRYLSAENRIVELS*AEGPPGGSL*G TLCRSP*CSGELLFVD*QPVHPVIEVGSQAFAT PSHSRHHLCV*VEHILL*KQEPWLLRTALVLPW LCLMEAEDKMVQHVTLSYRRAMPSLNAPKGAAGM *TPYG*LLGAPARAGCVCAE*AGPGPCPGMAQQ SPPPSHLPPCCLHPPGAEVCLPDPRPPVLCDGV CQRG*AVLPPVPGACLHRGAGPVLWCRDCLGS*V LALAGRGIPRHQAGKPHAGQRWPHQDH*LWPLQ RGHQ*RGHHENLLWDPGVPGA*GAGGQ*LWPGR GLVGAGCGHVRDDVRP

Frame 1

HQGQWPPVKWTRLRMAYVSFLYLSCCPGILLSH STFSNKIL*GTSVQKTE*LSCHELKDPLVGVSE ELSVDLLSAVENYCLLTDSLSILSLKLAPKPLPL PATADITCVCKLNTFYCKNKSHGCSEQL*FSLG CA*WRQRTRWFSM*PFPTAEPCLHLMPQKVQPV CEPHMADSWGPQPGQGVCVQNKQGQGPALAWLSR ALLPPTSHPAAFIPQALKYAFQTHDRLCFVMEY ANGGELFFHLSRERVFTEERARFYGAEIVSALE YLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCK EGISDGATMKTFCGTPEYLAPEVLEDNDYGRAV DWWGLGVVMYEMMCGR

Frame 2

IKASGHQLNGLD*GWLMYHFFISVAVQVSY*AT VLFQIKSYEVPQCRKQNS*VVMS*RTPWWESLR NSL*ISLVQWRTTVC*LTACPSCH*SWLPSLCH SQPQQTSLVCVS*THFTVKTRAMVAQNSSSSPLA VPDGGRGQDGSACNPFLPQSHAFT*CPKRCSRY VNPIWLTPGGPSQGRVCVCRISRARALPWHGSA EPSSLPPPTLLPSSPRR*SMPSRPTTACAL*WSM PTGVSCSSTCPGSVSSQRSGPGFMVQRLSRLLS TCTRGTWYTATSSWKTSCWTKMATSRSLTLASA KRASVTGPP*KPSVGPRSTWRLRCWRTMTMAGPW TGGGWVWSCTR*CAA

Sequence AKT2.oAug05

AGCTGTGTGCACAGGAGAAGTGGGGACACACACTCACCTTAGAAAGTGTCCCCCTCACTCAGCAGCAGGACTTACTCTAGCCCCAACTGGAACAACCCAA GTATCCACTGTGAGAGGAACAGTCAGATCCACTGTGGAGTGTTCTCACAGTGGGACGCCACACAGCAGTTAGGAATGAGCTACAGCTACACACGACAGTG TGGACGCGTCTCATAGGCTAATGTCGAGGGAAAGCCGCACAGAGGAGCTGGAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGG CCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTGAGGTGCTGGAGGACAATGACTATGGC CGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGC

AKT2.F10 (forward primer)
AKT2.R10 (reverse primer)
Alternative donor

Frame 0

SCVHRRSGDTHSP*KVSPSLSSRTYSSPNWNNP SIHCERNSQIHCGVFSQWDATQQLGMSYSYTRQC GRVS*ANVEGKPHRGAGKPHAGQRWPHQDH*LW PLQRGHQ*RGHHENLLWDPGVPGA*GAGGQ*LW PGRGLVGAGCGHVRDDVRP

Frame 1

AVCTGEVGTHTHLRKCPPHSAAGLTLAPTGTTQ VSTVRGTVRSTVECSHSGTPHSS*E*ATATHDS VDASHRLMSRESRTEELENLMLDKDGHIKITDFG LCKEGISDGATMKTFCGTPEYLAPEVLEDNDYG RAVDWWGLGVVMYEMMCGR

Frame 2

LCAQEKWGHTLTLESVPLTQQQDLL*PQLEQPK YPL*EEQSDPLWSVLTVGRHTAVRNELQLHTTV WTRLIG*CRGKAAQRSWKTSCWTKMATSRSLTL ASAKRASVTGPP*KPSVGPRSTWRLRCWRTMTMA GPWTGGGWVWSCTR*CAA

Sequence AKT2.aAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.bAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.cAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.gAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.hAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.oAug05

Frame 0

Frame 1

Frame 2

Sequence AKT2.iAug05

Frame 0

Frame 1

Frame 2