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Sequence AKT2.iAug05
CCATCAAGGCCAGTGGCCACCAGTTAAATGGACTAGATTGAGGATGGCTTATGTATCATTTCTTTATCTCAGTTGCTGTCCAGGTATCCTATTAAGCCACAGTACTTTTTCAAATAAAATCTTATGAGGTACCTCAGTGCAGAAAACAGAATAGTTGAGTTGTCATGAGCTGAAGGACCCCCTGGTGGGAGTCTCTGAGG
AACTCTCTGTAGATCTCCTTAGTGCAGTGGAGAACTACTGTTTGTTGACTGACAGCCTGTCCATCCTGTCATTGAAGTTGGCTCCCAAGCCTTTGCCACT
CCCAGCCACAGCAGACATCACTTGTGTGTGTAAGTTGAACACATTTTACTGTAAAAACAAGAGCCATGGTTGCTCAGAACAGCTCTAGTTCTCCCTTGGC
TGTGCCTGATGGAGGCAGAGGACAAGATGGTTCAGCATGTAACCCTTTCCTACCGCAGAGCCATGCCTTCACTTAATGCCCCAAAAGGTGCAGCCGGTAT
GTGAACCCCATATGGCTGACTCCTGGGGGCCCCAGCCAGGGCAGGGTGTGTGTGTGCAGAATAAGCAGGGCCAGGGCCCTGCCCTGGCATGGCTCAGCAG
AGCCCTCCTCCCTCCCACCTCCCACCCTGCTGCCTTCATCCCCCAGGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTAT
GCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGT
ACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAA
AGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTGAGGTGTCTGGGGTTGGTGGGGGTGCTGGAGGACAA
TGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGC
- AKT2.F10 (forward primer)
- AKT2.R10 (reverse primer)
- Alternative donor and acceptor
Frame 0
PSRPVATS*MD*IEDGLCIISLSQLLSRYPIKPQYFFK*NLMRYLSAENRIVELS*AEGPPGGSL*G
TLCRSP*CSGELLFVD*QPVHPVIEVGSQAFAT
PSHSRHHLCV*VEHILL*KQEPWLLRTALVLPW
LCLMEAEDKMVQHVTLSYRRAMPSLNAPKGAAGM
*TPYG*LLGAPARAGCVCAE*AGPGPCPGMAQQ
SPPPSHLPPCCLHPPGAEVCLPDPRPPVLCDGV
CQRG*AVLPPVPGACLHRGAGPVLWCRDCLGS*V
LALAGRGIPRHQAGKPHAGQRWPHQDH*LWPLQ
RGHQ*RGHHENLLWDPGVPGA*GVWGWWGCWRT
MTMAGPWTGGGWVWSCTR*CAA
Frame 1
HQGQWPPVKWTRLRMAYVSFLYLSCCPGILLSHSTFSNKIL*GTSVQKTE*LSCHELKDPLVGVSE
ELSVDLLSAVENYCLLTDSLSILSLKLAPKPLPL
PATADITCVCKLNTFYCKNKSHGCSEQL*FSLG
CA*WRQRTRWFSM*PFPTAEPCLHLMPQKVQPV
CEPHMADSWGPQPGQGVCVQNKQGQGPALAWLSR
ALLPPTSHPAAFIPQALKYAFQTHDRLCFVMEY
ANGGELFFHLSRERVFTEERARFYGAEIVSALE
YLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCK
EGISDGATMKTFCGTPEYLAPEVSGVGGGAGGQ
*LWPGRGLVGAGCGHVRDDVRP
Frame 2
IKASGHQLNGLD*GWLMYHFFISVAVQVSY*ATVLFQIKSYEVPQCRKQNS*VVMS*RTPWWESLR
NSL*ISLVQWRTTVC*LTACPSCH*SWLPSLCH
SQPQQTSLVCVS*THFTVKTRAMVAQNSSSSPLA
VPDGGRGQDGSACNPFLPQSHAFT*CPKRCSRY
VNPIWLTPGGPSQGRVCVCRISRARALPWHGSA
EPSSLPPPTLLPSSPRR*SMPSRPTTACAL*WSM
PTGVSCSSTCPGSVSSQRSGPGFMVQRLSRLLS
TCTRGTWYTATSSWKTSCWTKMATSRSLTLASA
KRASVTGPP*KPSVGPRSTWRLRCLGLVGVLEDN
DYGRAVDWWGLGVVMYEMMCGR
Sequence AKT2.dAug05
ACTTCTCTGGGCGCCACCGTAGCTCCCGACCTGCTGTCCGCAAATAACCCCAGGCTGGGAAAGGCAAAGGCGCCTTGGTCTCTGTGGACCCTGGCCAGGCCCCAGGCTCCAGGTGAATACATCAAGACCTGGAGGCCACGGTACTTCCTGCTGAAGAGCGACGGCTCCTTCATTGGGTACAAGGAGAGGCCCGAGGCCCC
TGATCAGACTCTACCCCCCTTAAACAACTTCTCCGTAGCAGAATGCCAGCTGATGAAGACCGAGAGGCCGCGACCCAACACCTTTGTCATACGCTGCCTG
CAGTGGACCACAGTCATCGAGAGGACCTTCCACGTGGATTCTCCAGACGAGAGGGAGGAGTGGATGCGGGCCATCCAGATGGTCGCCAACAGCCTCAAGC
AGCGGGCCCCAGGCGAGGACCCCATGGACTACAAGTGTGGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTCAGCAAGGCACGGGC
TAAAGTGACCATGAATGACTTCGACTATCTCAAACTCCTTGGCAAGGGAACCTTTGGCAAAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTAC
GCCATGAAGATCCTGCGGAAGGAAGTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAGAGCCGGGTCCTCCAGAACACCAGGCACCCGTTCC
TCACTGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCACCTGTCCCGGGAGCG
TGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTCTCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTG
GAAAACCTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGCATCAGTGACGGGGCCACCATGAAAACCTTCTGTG
GGACCCCGGAGTACCTGGCGCCTGAGGGCCACGGGCCCAGCCCTCATTTCTCCTCCATCCTCAGGTGCTGGAGGACAATGACTATGGCCGGGCCGTGGAC
TGGTGGGGGCTGGGTGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAAG
AGATCCGCTTCCCGCGCACGCTCAGCCCCGAGGCCAAGTCCCTGCTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGTGGTTTGTGGTCAAGACAGAA
TCATTAAGGGCCATACAGGGCCTGCCTGCTCCGAAAGCCCGTCTGCCCGAACCCAGCTGCTCCGGGAGGCAGGTGCTGACGGCCGTCTCTGCACAGGCTT
GGTGGGGGGCCCAGCGATGCCAAGGAGGTCATGGAGCACAGGTTCTTCCTCAGCATCAACTGGCAGGACGTGGTCCAGAAGAAGCTCCTGCCACCCTTCA
AACCTCAGGTCACGTCCGAGGTCGACACAAGGTACTTCGATGATGAATTTACCGCCCAGTCCATCACAATCACACCCCCTGACCGCTGTGAGTGCCTGGG
GCCCCCGCGCTGGTGTGCCTGCCCCAGGGGTGGAGGGATGGGATCTGCTCTTAACTCACTTCTCAA
- AKT2.F10 (forward primer)
- AKT2.R10 (reverse primer)
- Alternative donor and acceptor
Frame 0
TSLGATVAPDLLSANNPRLGKAKAPWSLWTLARPQAPGEYIKTWRPRYFLLKSDGSFIGYKERPEAP
DQTLPPLNNFSVAECQLMKTERPRPNTFVIRCL
QWTTVIERTFHVDSPDEREEWMRAIQMVANSLK
QRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARA
KVTMNDFDYLKLLGKGTFGKVILVREKATGRYY
AMKILRKEVIIAKDEVAHTVTESRVLQNTRHPF
LTALKYAFQTHDRLCFVMEYANGGELFFHLSRER
VFTEERARFYGAEIVSALEYLHSRDVVYRDIKL
ENLMLDKDGHIKITDFGLCKEGISDGATMKTFC
GTPEYLAPEGHGPSPHFSSILRCWRTMTMAGPWT
GGGWVWSCTR*CAAACPSTTRTTSASSSSSSWK
RSASRARSAPRPSPCLLGCLRRTPSRVVCGQDR
IIKGHTGPACSESPSARTQLLREAGADGRLCTGL
VGGPAMPRRSWSTGSSSASTGRTWSRRSSCHPS
NLRSRPRSTQGTSMMNLPPSPSQSHPLTAVSAW
GPRAGVPAPGVEGWDLLLTHFS
Frame 1
LLWAPP*LPTCCPQITPGWERQRRLGLCGPWPGPRLQVNTSRPGGHGTSC*RATAPSLGTRRGPRP
LIRLYPP*TTSP*QNAS**RPRGRDPTPLSYAAC
SGPQSSRGPSTWILQTRGRSGCGPSRWSPTASS
SGPQARTPWTTSVAPPVTPPRLRRWKWRSARHG
LK*P*MTSTISNSLAREPLAKSSWCGRRPLAATT
P*RSCGRKSSLPRMKSLTQSPRAGSSRTPGTRS
SLR*SMPSRPTTACAL*WSMPTGVSCSSTCPGS
VSSQRSGPGFMVQRLSRLLSTCTRGTWYTATSSW
KTSCWTKMATSRSLTLASAKRASVTGPP*KPSV
GPRSTWRLRATGPALISPPSSGAGGQ*LWPGRG
LVGAGCGHVRDDVRPPALLQPGPRAPLRAHPHGR
DPLPAHAQPRGQVPACWAA*EGPQAEWFVVKTE
SLRAIQGLPAPKARLPEPSCSGRQVLTAVSAQA
WWGAQRCQGGHGAQVLPQHQLAGRGPEEAPATLQ
TSGHVRGRHKVLR**IYRPVHHNHTP*PL*VPG
APALVCLPQGWRDGICS*LTSQ
Frame 2
FSGRHRSSRPAVRK*PQAGKGKGALVSVDPGQAPGSR*IHQDLEATVLPAEERRLLHWVQGEARGP
*SDSTPLKQLLRSRMPADEDREAATQHLCHTLP
AVDHSHREDLPRGFSRREGGVDAGHPDGRQQPQA
AGPRRGPHGLQVWLPQ*LLHD*GDGSGGQQGTG
*SDHE*LRLSQTPWQGNLWQSHPGAGEGHWPLL
RHEDPAEGSHHCQG*SRSHSHREPGPPEHQAPVP
HCAEVCLPDPRPPVLCDGVCQRG*AVLPPVPGA
CLHRGAGPVLWCRDCLGS*VLALAGRGIPRHQA
GKPHAGQRWPHQDH*LWPLQRGHQ*RGHHENLLW
DPGVPGA*GPRAQPSFLLHPQVLEDNDYGRAVD
WWGLGVVMYEMMCGRLPFYNQDHERLFELILME
EIRFPRTLSPEAKSLLAGLLKKDPKQSGLWSRQN
H*GPYRACLLRKPVCPNPAAPGGRC*RPSLHRL
GGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPF
KPQVTSEVDTRYFDDEFTAQSITITPPDRCECLG
PPRWCACPRGGGMGSALNSLL
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