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Sequences

Sequence CHEK2.aAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGC
CATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGGTGTGAGGCACCATCCCCGGC
CAGTTTGCAGTCTAAAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAA
ATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACC
TTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTT
TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA
GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC
AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG
AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT
GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT
TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG
CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC
ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA
GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG
AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG
CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT
TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG
ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG
CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT
ATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*DRVW
PCYPI*S*TPAVI*SSCLSLPKCWDKRCEAPSPA
SLQSKMCE*QLLVWEGQKL*ILL**TTAEKNR*
IPNIQQETLSDFQGSGS*KLLHCIHRRSQWQWN
LCKYRACRERKTPSFE*QF*NCTVTKQK*SFCLF
*SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK
AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS
QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG
IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC
AVPS*KRYYTP*LKARECFTVISRRGLSYKDY*
FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC
WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV
TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG
SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G
K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA
PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV
FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA*
*SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW
LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF
YIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLETESG
HVTQSDLELLLSSDPPASASQSAGIRGVRHHPR
PVCSLKCVNDNYWFGRDKSCEYCFDEPLLKRTDK
YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGT
FVNTELVGKGKRRPLNNNSEIALSLSRNKVFVF
FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK
LAFERKTCKKVAIKIISKRKFAIGSAREADPAL
NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL
ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV
QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD
FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT
AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS
LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV
VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE
ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR
PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF
FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL
DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG
SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF
IYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILRQSLA
MLPNLILNSCCHLILLPQPPKVLG*EV*GTIPG
QFAV*NV*MTTTGLGGTKAVNIALMNHC*KEQI
NTEHTARNTFGFSGKWVLKTLTLHT*KITVAMEP
L*IQSL*GKENAVL*ITILKLHCH*AEIKFLSF
LI*L*MISQFILRH*EMNTSCQKLLEVVPVER*
SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS
MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW
N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL
CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI
LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL
LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC
H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW*
WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR
KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS
AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF
FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL
IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL
AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL
YIL

Sequence CHEK2.mAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT
CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA
GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGG
CAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTT
TTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTG
GAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGA
CCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCT
GAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAAT
GTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTG
GAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTAT
CTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGG
GCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGC
TTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCG
AAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGA
AATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTG
ATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAG
TCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTC
CTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS
SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK
RTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSG
NGTFVNTELVGKGKRRPLNNNSEIALSLSRNKV
FVFFDLTVDDQSVYPKALRDEYIMSKTLGSGAC
GEVKLAFERKTCKKVAIKIISKRKFAIGSAREAD
PALNVETEIEILKKLNHDGRGRAV*QSGGE*TP
ERSYLQALFLPDALGCAVPS*KRYYTP*LKARE
CFTVISRRGLSYKDY*FWALQDFGRDLSHENLMW
NPHLLGA*SSCFCWDCWV*PCCGLLEFRSYSFY
LP*WVSTFL*A*DSSVTEGSDHQWKIQLHS*SL
GRSLRESSGPCQEVVGSGSKGTFYDRRSLKTPVA
SG*RHEEKVSRSSV*GK*IHSSTPGSSPAFY*S
KAAP*RGSRGCRDHKAPSCVCCCVVNSVV*TRK
KCTFFHSVIFLFFESVFL*FVF*LWE*LLFHSH*
CTIKNLMEPGLCASA**SVL*LNGFIIYLFEME
SHSVAQPE*SAVVQAWLTATSASQVQAILVPQP
PE*LGLQVCTTMPVNFYIF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP
PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K
EQINTEHTARNTFGFSGKWVLKTLTLHT*KITV
AMEPL*IQSL*GKENAVL*ITILKLHCH*AEIKF
LSFLI*L*MISQFILRH*EMNTSCQKLLEVVPV
ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQ
TQLSMLKQK*KF*KS*IMMEGGELFDKVVGNKRL
KEATCKLYFYQMLLAVQYLHENGIIHRDLKPEN
VLLSSQEEDCLIKITDFGHSKILGETSLMRTLC
GTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFI
CLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVW
AEVSEKALDLVKKLLVVDPKARFTTEEALRHPW
LQDEDMKRKFQDLLSEENESTALPQVLAQPSTSR
KRPREGEAEGAETTKRPAVCAAVL*TPWFEHER
NVPSFTLSSFFSLSLFFYSLYFNYGNNCFFTVT
DVQLKT*WNLGFVLLLDNRFFS*MALLFIYLRWS
LTLLPSLNEVQWCRLGSLQPLLPRFKRFLCLSL
LSSWDYRYAPPCLLIFIYF

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL
LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK
NR*IPNIQQETLSDFQGSGS*KLLHCIHRRSQW
QWNLCKYRACRERKTPSFE*QF*NCTVTKQK*S
FCLF*SDCR*SVSLS*GIKR*IHHVKNSWKWCLW
RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR
PSSQC*NRNRNFEKAKS*WKGESCLTKWWGINA
*KKLPASSIFTRCSWLCSTFMKTVLYTVT*SQRM
FYCHLKKRTVL*RLLILGTPRFWERPLS*EPYV
EPPPTWRLKFLFLLGLLGITVLWTAGV*ELFFL
SALVGIHLSLSIGLKCH*RIRSPVENTTSFLKSG
QKSQRKLWTLSRSCW*WIQRHVLRQKKP*DTRG
FRMKT*RESFKIFCLRKMNPQLYPRF*PSLLLV
ESGPVKGKPRVPRPQSAQLCVLLCCELRGLNTKE
MYLLSLCHLSFL*VCFFIVCILIMGIIAFSQSL
MYN*KPDGTWALCFCLIIGSLVEWLYYLFI*DG
VSLCCPA*MKCSGAGLAHCNLCFPGSSDSCASAS
*VAGITGMHHHAC*FLYIL

Sequence CHEK2.gAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGATACTGGATTTCACCATGGTGCCCAGGCTGGTCTTGAAT
TCCTGGGCTCAAGCAATCTACCTGCGTTGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACCACACCTGGCAGAACCTTTGTGAAAGATCTCAATT
TAATATGGAAATAAAAAAATTTGTATGCTGAGGCTGCTAACGTCTATGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCA
GTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTC
CAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGAC
CAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTG
GGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGA
AGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCT
TTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCAT
TAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAA
GATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCAT
CCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGA
ATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTC
TGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACA
AAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTC
TGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCT
GCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGG
CTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAA
TTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRDTGFHHGAQAGLE
FLGSSNLPALASQSAGITDMNHHTWQNLCERSQF
NMEIKKFVC*GC*RLWS*CLGSRMLRLSSLMAA
VPVHSPMAALPSPKAPPHSPRAYPAPLPARCQT
PASPLTPALGH*AP*RQCPLRNSILFLRTKNLRT
KNLRSLPLPPGLDYGPFRMDLPILNV*MTTTGL
GGTKAVNIALMNHC*KEQINTEHTARNTFGFSG
KWVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL
*ITILKLHCH*AEIKFLSFLI*L*MISQFILRH
*EMNTSCQKLLEVVPVER*SWLSRGKHVRK*P*
RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*II
LASSRLKTFLMQKIIILFWN*WKGESCLTKWWG
INA*KKLPASSIFTRCSWLCRMKT*RESFKIFC
LRKMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQ
SAQLCVLLCCELRGLNTKEMYLLSLCHLSFL*V
CFFIVCILIMGIIAFSQSLMYN*KPDGTWALCF
CLIIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAG
LAHCNLCFPGSSDSCASAS*VAGITGMHHHAC*
FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAILDFTMVPRLVLN
SWAQAIYLRWPPKVLGLQT*TTTPGRTFVKDLN
LIWK*KNLYAEAANVYGRDVSGVGC*GSAVSWQQ
CLFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKL
QPVLSLQLWDTELLRDSVHSGTLFYS*GPRT*G
PRT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVW
EGQKL*ILL**TTAEKNR*IPNIQQETLSDFQG
SGS*KLLHCIHRRSQWQWNLCKYRACRERKTPS
FE*QF*NCTVTKQK*SFCLF*SDCR*SVSLS*GI
KR*IHHVKNSWKWCLWRGKAGFREENM*ESSHK
DHQQKEVCYWFSKRGRPSSQC*NRNRNFEKAKS
SLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSGGE
*TPERSYLQALFLPDALGCAG*RHEEKVSRSSV
*GK*IHSSTPGSSPAFY*SKAAP*RGSRGCRDH
KAPSCVCCCVVNSVV*TRKKCTFFHSVIFLFFES
VFL*FVF*LWE*LLFHSH*CTIKNLMEPGLCAS
A**SVL*LNGFIIYLFEMESHSVAQPE*SAVVQ
AWLTATSASQVQAILVPQPPE*LGLQVCTTMPVN
FYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRYWISPWCPGWS*I
PGLKQSTCVGLPKCWDYRHEPPHLAEPL*KISI
*YGNKKICMLRLLTSMVVMSRESDVEAQQSHGS
SACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNS
SQSSHSSSGTLSSLETVSTQELYSIPEDQEPED
QEPEEPTPAPWARLWALQDGFANLECVNDNYWF
GRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFRE
VGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRP
LNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKA
LRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIK
IISKRKFAIGSAREADPALNVETEIEILKKLNH
PCIIKIKNFFDAEDYYIVLELMEGGELFDKVVG
NKRLKEATCKLYFYQMLLAVQDEDMKRKFQDLLS
EENESTALPQVLAQPSTSRKRPREGEAEGAETT
KRPAVCAAVL*TPWFEHERNVPSFTLSSFFSLS
LFFYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLL
LDNRFFS*MALLFIYLRWSLTLLPSLNEVQWCR
LGSLQPLLPRFKRFLCLSLLSSWDYRYAPPCLL
IFIYF

Sequence CHEK2.eAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG
AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT
TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA
AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA
AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC
AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGATTACTGATTTTGGGCACTCCAAGA
TTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGC
TGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATC
ACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCAC
GTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGC
TCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCT
GCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATT
TTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAG
TTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTG
CTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER
KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS
LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E
SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE
KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*
QSGGE*TPERSYLQALFLPDALGCADY*FWALQD
FGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PC
CGLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSD
HQWKIQLHS*SLGRSLRESSGPCQEVVGSGSKGT
FYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHS
STPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVC
CCVVNSVV*TRKKCTFFHSVIFLFFESVFL*FVF
*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*
LNGFIIYLFEMESHSVAQPE*SAVVQAWLTATS
ASQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG
KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV
YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK
KVAIKIISKRKFAIGSAREADPALNVETEIEILK
KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD
KVVGNKRLKEATCKLYFYQMLLAVQITDFGHSK
ILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRA
VDCWSLGVILFICLSGYPPFSEHRTQVSLKDQI
TSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKA
RFTTEEALRHPWLQDEDMKRKFQDLLSEENESTA
LPQVLAQPSTSRKRPREGEAEGAETTKRPAVCA
AVL*TPWFEHERNVPSFTLSSFFSLSLFFYSLY
FNYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFFS
*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPL
LPRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK
ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF
ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR
K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*
KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT
KWWGINA*KKLPASSIFTRCSWLCRLLILGTPR
FWERPLS*EPYVEPPPTWRLKFLFLLGLLGITV
LWTAGV*ELFFLSALVGIHLSLSIGLKCH*RIRS
PVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRH
VLRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQ
LYPRF*PSLLLVESGPVKGKPRVPRPQSAQLCVL
LCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCI
LIMGIIAFSQSLMYN*KPDGTWALCFCLIIGSL
VEWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNLC
FPGSSDSCASAS*VAGITGMHHHAC*FLYIL

Sequence CHEK2.dAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG
AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT
TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA
AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA
AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC
AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATAC
ACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGAC
CTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGG
AGTTTAGGAGTTATTCTTTTTATCTGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCT
CAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGA
AGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCC
CGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTT
CTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATT
AAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTG
TTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCT
GGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER
KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS
LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E
SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE
KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*
QSGGE*TPERSYLQALFLPDALGCAVPS*KRYYT
P*LKARECFTVISRRGLSYKDY*FWALQDFGRD
LSHENLMWNPHLLGA*SSCFCWDCWV*PCCGLL
EFRSYSFYLDSSVTEGSDHQWKIQLHS*SLGRSL
RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG*
RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAA
P*RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF
FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI
KNLMEPGLCASA**SVL*LNGFIIYLFEMESHS
VAQPE*SAVVQAWLTATSASQVQAILVPQPPE*L
GLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG
KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV
YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK
KVAIKIISKRKFAIGSAREADPALNVETEIEILK
KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD
KVVGNKRLKEATCKLYFYQMLLAVQYLHENGII
HRDLKPENVLLSSQEEDCLIKITDFGHSKILGET
SLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCW
SLGVILFIWTQVSLKDQITSGKYNFIPEVWAEV
SEKALDLVKKLLVVDPKARFTTEEALRHPWLQDE
DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP
REGEAEGAETTKRPAVCAAVL*TPWFEHERNVP
SFTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL
KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL
LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSS
WDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK
ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF
ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR
K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*
KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT
KWWGINA*KKLPASSIFTRCSWLCSTFMKTVLY
TVT*SQRMFYCHLKKRTVL*RLLILGTPRFWER
PLS*EPYVEPPPTWRLKFLFLLGLLGITVLWTAG
V*ELFFLSGLKCH*RIRSPVENTTSFLKSGQKS
QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRM
KT*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP
VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL
LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN
*KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC
CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA
GITGMHHHAC*FLYIL

Sequence CHEK2.cAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG
AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT
TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA
AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA
AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC
AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATAC
ACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGAC
CTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGG
AGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAAT
ACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGA
AGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTT
CTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAA
CTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAA
TAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTA
TTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCA
AGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER
KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS
LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E
SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE
KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*
QSGGE*TPERSYLQALFLPDALGCAVPS*KRYYT
P*LKARECFTVISRRGLSYKDY*FWALQDFGRD
LSHENLMWNPHLLGA*SSCFCWDCWV*PCCGLL
EFRSYSFYLP*WVSTFL*A*DSSVTEGSDHQWKI
QLHS*SLGRSLRESSGPCQEVVGSGSKGTFYDR
RSLKTPVASG*RHEEKVSRSSV*GK*IHSSTPG
SSPAFY*SKAAP*RGSRGCRDHKAPSCVCCCVVN
SVV*TRKKCTFFHSVIFLFFESVFL*FVF*LWE
*LLFHSH*CTIKNLMEPGLCASA**SVL*LNGF
IIYLFEMESHSVAQPE*SAVVQAWLTATSASQVQ
AILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG
KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV
YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK
KVAIKIISKRKFAIGSAREADPALNVETEIEILK
KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD
KVVGNKRLKEATCKLYFYQMLLAVQYLHENGII
HRDLKPENVLLSSQEEDCLIKITDFGHSKILGET
SLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCW
SLGVILFICLSGYPPFSEHRTQVSLKDQITSGK
YNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTE
EALRHPWLQDEDMKRKFQDLLSEENESTALPQV
LAQPSTSRKRPREGEAEGAETTKRPAVCAAVL*
TPWFEHERNVPSFTLSSFFSLSLFFYSLYFNYGN
NCFFTVTDVQLKT*WNLGFVLLLDNRFFS*MAL
LFIYLRWSLTLLPSLNEVQWCRLGSLQPLLPRF
KRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK
ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF
ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR
K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*
KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT
KWWGINA*KKLPASSIFTRCSWLCSTFMKTVLY
TVT*SQRMFYCHLKKRTVL*RLLILGTPRFWER
PLS*EPYVEPPPTWRLKFLFLLGLLGITVLWTAG
V*ELFFLSALVGIHLSLSIGLKCH*RIRSPVEN
TTSFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQ
KKP*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF
*PSLLLVESGPVKGKPRVPRPQSAQLCVLLCCE
LRGLNTKEMYLLSLCHLSFL*VCFFIVCILIMG
IIAFSQSLMYN*KPDGTWALCFCLIIGSLVEWLY
YLFI*DGVSLCCPA*MKCSGAGLAHCNLCFPGS
SDSCASAS*VAGITGMHHHAC*FLYIL

Sequence CHEK2.bAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGATACTGGATTTCACCATGGTGCCCAGGCTGGTCTTGAAT
TCCTGGGCTCAAGCAATCTACCTGCGTTGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACCACACCTGGCAGAACCTTTGTATCTTGAAAGCTGT
GTAAATACAATCAGTGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCC
AGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACT
GAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGG
GCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATG
AACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACAT
AGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTA
AGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTG
GAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTC
AGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGAT
GCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCT
ATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGA
GGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCT
GAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTT
TCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCT
GGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGA
AAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAG
CCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTC
ATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGG
AACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGA
ATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTA
TGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRDTGFHHGAQAGLE
FLGSSNLPALASQSAGITDMNHHTWQNLCILKAV
*IQSVS*CLGSRMLRLSSLMAAVPVHSPMAALP
SPKAPPHSPRAYPAPLPARCQTPASPLTPALGH
*AP*RQCPLRNSILFLRTKNLRTKNLRSLPLPPG
LDYGPFRMDLPILNV*MTTTGLGGTKAVNIALM
NHC*KEQINTEHTARNTFGFSGKWVLKTLTLHT
*KITVAMEPL*IQSL*GKENAVL*ITILKLHCH*
AEIKFLSFLI*L*MISQFILRH*EMNTSCQKLL
EVVPVER*SWLSRGKHVRK*P*RSSAKGSLLLV
QQERQTQLSMLKQK*KF*KS*IILASSRLKTFLM
QKIIILFWN*WKGESCLTKWWGINA*KKLPASS
IFTRCSWLCSTFMKTVLYTVT*SQRMFYCHLKK
RTVL*RLLILGTPRFWERPLS*EPYVEPPPTWRL
KFLFLLGLLGITVLWTAGV*ELFFLSALVGIHL
SLSIGLKCH*RIRSPVENTTSFLKSGQKSQRKL
WTLSRSCW*WIQRHVLRQKKP*DTRGFRMKT*RE
SFKIFCLRKMNPQLYPRF*PSLLLVESGPVKGK
PRVPRPQSAQLCVLLCCELRGLNTKEMYLLSLC
HLSFL*VCFFIVCILIMGIIAFSQSLMYN*KPDG
TWALCFCLIIGSLVEWLYYLFI*DGVSLCCPA*
MKCSGAGLAHCNLCFPGSSDSCASAS*VAGITG
MHHHAC*FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAILDFTMVPRLVLN
SWAQAIYLRWPPKVLGLQT*TTTPGRTFVS*KL
CKYNQCRDVSGVGC*GSAVSWQQCLFTAPWQRYP
VPRLLLTVPGHIQLLYQHDAKLQPVLSLQLWDT
ELLRDSVHSGTLFYS*GPRT*GPRT*GAYPCPL
GSIMGPSGWICQS*MCE*QLLVWEGQKL*ILL**
TTAEKNR*IPNIQQETLSDFQGSGS*KLLHCIH
RRSQWQWNLCKYRACRERKTPSFE*QF*NCTVT
KQK*SFCLF*SDCR*SVSLS*GIKR*IHHVKNSW
KWCLWRGKAGFREENM*ESSHKDHQQKEVCYWF
SKRGRPSSQC*NRNRNFEKAKSSLHHQD*KLF*
CRRLLYCFGIDGRGRAV*QSGGE*TPERSYLQAL
FLPDALGCAVPS*KRYYTP*LKARECFTVISRR
GLSYKDY*FWALQDFGRDLSHENLMWNPHLLGA
*SSCFCWDCWV*PCCGLLEFRSYSFYLP*WVSTF
L*A*DSSVTEGSDHQWKIQLHS*SLGRSLRESS
GPCQEVVGSGSKGTFYDRRSLKTPVASG*RHEE
KVSRSSV*GK*IHSSTPGSSPAFY*SKAAP*RGS
RGCRDHKAPSCVCCCVVNSVV*TRKKCTFFHSV
IFLFFESVFL*FVF*LWE*LLFHSH*CTIKNLM
EPGLCASA**SVL*LNGFIIYLFEMESHSVAQPE
*SAVVQAWLTATSASQVQAILVPQPPE*LGLQV
CTTMPVNFYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRYWISPWCPGWS*I
PGLKQSTCVGLPKCWDYRHEPPHLAEPLYLESC
VNTISVVMSRESDVEAQQSHGSSACSQPHGSVT
QSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTL
SSLETVSTQELYSIPEDQEPEDQEPEEPTPAPW
ARLWALQDGFANLECVNDNYWFGRDKSCEYCFD
EPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYI
EDHSGNGTFVNTELVGKGKRRPLNNNSEIALSL
SRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTL
GSGACGEVKLAFERKTCKKVAIKIISKRKFAIGS
AREADPALNVETEIEILKKLNHPCIIKIKNFFD
AEDYYIVLELMEGGELFDKVVGNKRLKEATCKL
YFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEE
DCLIKITDFGHSKILGETSLMRTLCGTPTYLAP
EVLVSVGTAGYNRAVDCWSLGVILFICLSGYPP
FSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKAL
DLVKKLLVVDPKARFTTEEALRHPWLQDEDMKR
KFQDLLSEENESTALPQVLAQPSTSRKRPREGE
AEGAETTKRPAVCAAVL*TPWFEHERNVPSFTLS
SFFSLSLFFYSLYFNYGNNCFFTVTDVQLKT*W
NLGFVLLLDNRFFS*MALLFIYLRWSLTLLPSL
NEVQWCRLGSLQPLLPRFKRFLCLSLLSSWDYRY
APPCLLIFIYF

Sequence CHEK2.hAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAAGTGAGTAAAGATGAGTAAGTTGCTGGGCGTGGTGGCTCACACCTGTAATCC
CAGCACTTTGGGAGGCTGAGGCGGATGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACCCACATGGAGAAACCCTGTCTCTACTAAAAATACAAA
ATAAGCCGGGCATGGTGGTGCATGCCTGTAATCCCAGCTATTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCA
AGATCGTGCCATTGCACTCTAGCCTGGGCAACAAGAGTGAAACTCTGTCTCAAAACAAAAAAAAAGATGATTTTTGTCTTTTTTGATCTGACTGTAGATG
ATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAA
AACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATA
GAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAG
AGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAA
CGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATT
TTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTG
TGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCAC
CAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGT
TTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTC
TACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGC
TGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTT
AATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTT
GAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCT
TCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGGKFVLSLQNMV*VSKDE*VAGRGGSHL*S
QHFGRLRRMDHLRSGVRDQPDPHGETLSLLKIQ
NKPGMVVHACNPSYSGG*GRRIA*TREAEVAVSQ
DRAIAL*PGQQE*NSVSKQKKR*FLSFLI*L*M
ISQFILRH*EMNTSCQKLLEVVPVER*SWLSRG
KHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK*
KF*KS*IILASSRLKTFLMQKIIILFWN*WKGE
SCLTKWWGINA*KKLPASSIFTRCSWLCSTFMK
TVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPRF
WERPLS*EPYVEPPPTWRLKFLFLLGLLGITVL
WTAGV*ELFFLSALVGIHLSLSIGLKCH*RIRS
PVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRHV
LRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQL
YPRF*PSLLLVESGPVKGKPRVPRPQSAQLCVL
LCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCIL
IMGIIAFSQSLMYN*KPDGTWALCFCLIIGSLV
EWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNLC
FPGSSDSCASAS*VAGITGMHHHAC*FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREENLSCPYRIWFK*VKMSKLLGVVAHTCNP
STLGG*GGWIT*GQEFETSLTHMEKPCLY*KYK
ISRAWWCMPVIPAIREAEAGESLEPGRQRLQ*A
KIVPLHSSLGNKSETLSQNKKKDDFCLF*SDCR*
SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFREE
NM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NRN
RNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRGR
AV*QSGGE*TPERSYLQALFLPDALGCAVPS*K
RYYTP*LKARECFTVISRRGLSYKDY*FWALQD
FGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PCC
GLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSDH
QWKIQLHS*SLGRSLRESSGPCQEVVGSGSKGT
FYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHSS
TPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVCC
CVVNSVV*TRKKCTFFHSVIFLFFESVFL*FVF
*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*L
NGFIIYLFEMESHSVAQPE*SAVVQAWLTATSA
SQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGRKICPVLTEYGLSE*R*VSCWAWWLTPVI
PALWEAEADGSPEVRSSRPA*PTWRNPVSTKNTK
*AGHGGACL*SQLFGRLRQENRLNPGGRGCSEP
RSCHCTLAWATRVKLCLKTKKKMIFVFFDLTVD
DQSVYPKALRDEYIMSKTLGSGACGEVKLAFERK
TCKKVAIKIISKRKFAIGSAREADPALNVETEI
EILKKLNHPCIIKIKNFFDAEDYYIVLELMEGG
ELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHEN
GIIHRDLKPENVLLSSQEEDCLIKITDFGHSKI
LGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRA
VDCWSLGVILFICLSGYPPFSEHRTQVSLKDQIT
SGKYNFIPEVWAEVSEKALDLVKKLLVVDPKAR
FTTEEALRHPWLQDEDMKRKFQDLLSEENESTA
LPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAA
VL*TPWFEHERNVPSFTLSSFFSLSLFFYSLYF
NYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFFS
*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPLL
PRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

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