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Sequences

Sequence CHEK2.kAug05

CAAGTGATCCTCCCACCTCAGCTTCCCAAGTAGCTGGGACTACAGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTG
CCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAG
CCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAA
GAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGA
GGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGT
GGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTG
AATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGC
CATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGG
TGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGA
CTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAG
GAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTT
CATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCC
AGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTG
GTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGC
TTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTA
TTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATT
CTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

QVILPPQLPK*LGLQVVMSRESDVEAQQSHGSS
ACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQ
EPEEPTPAPWARLWALQDGFANLECVNDNYWFG
RDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV
GPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPL
NNNSEIALSLSRNKVVPVER*SWLSRGKHVRK*
P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*
IILASSRLKTFLMQKIIILFWN*WKGESCLTKW
WGINA*KKLPASSIFTRCSWLCSTFMKTVLYTV
T*SQRMFYCHLKKRTVL*RLLILGTPRFWERPLS
*EPYVEPPPTWRLKFLFLLGLLGITVLWTAGV*
ELFFLSALVGIHLSLSIGLKCH*RIRSPVENTT
SFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQKKP
*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF*P
SLLLVESGPVKGKPRVPRPQSAQLCVLLCCELR
GLNTKEMYLLSLCHLSFL*VCFFIVCILIMGIIA
FSQSLMYN*KPDGTWALCFCLIIGSLVEWLYYL
FI*DGVSLCCPA*MKCSGAGLAHCNLCFPGSSD
SCASAS*VAGITGMHHHAC*FLYIL

Frame 1

K*SSHLSFPSSWDYRS*CLGSRMLRLSSLMAAV
PVHSPMAALPSPKAPPHSPRAYPAPLPARCQTP
ASPLTPALGH*AP*RQCPLRNSILFLRTKNLRTK
NLRSLPLPPGLDYGPFRMDLPILNV*MTTTGLG
GTKAVNIALMNHC*KEQINTEHTARNTFGFSGK
WVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL*
ITILKLHCH*AEIKWCLWRGKAGFREENM*ESS
HKDHQQKEVCYWFSKRGRPSSQC*NRNRNFEKA
KSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSG
GE*TPERSYLQALFLPDALGCAVPS*KRYYTP*
LKARECFTVISRRGLSYKDY*FWALQDFGRDLS
HENLMWNPHLLGA*SSCFCWDCWV*PCCGLLEFR
SYSFYLP*WVSTFL*A*DSSVTEGSDHQWKIQL
HS*SLGRSLRESSGPCQEVVGSGSKGTFYDRRS
LKTPVASG*RHEEKVSRSSV*GK*IHSSTPGSSP
AFY*SKAAP*RGSRGCRDHKAPSCVCCCVVNSV
V*TRKKCTFFHSVIFLFFESVFL*FVF*LWE*L
LFHSH*CTIKNLMEPGLCASA**SVL*LNGFIIY
LFEMESHSVAQPE*SAVVQAWLTATSASQVQAI
LVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

SDPPTSASQVAGTTGRDVSGVGC*GSAVSWQQC
LFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQ
PVLSLQLWDTELLRDSVHSGTLFYS*GPRT*GP
RT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVWE
GQKL*ILL**TTAEKNR*IPNIQQETLSDFQGS
GS*KLLHCIHRRSQWQWNLCKYRACRERKTPSF
E*QF*NCTVTKQK*SGACGEVKLAFERKTCKKVA
IKIISKRKFAIGSAREADPALNVETEIEILKKL
NHPCIIKIKNFFDAEDYYIVLELMEGGELFDKV
VGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRD
LKPENVLLSSQEEDCLIKITDFGHSKILGETSL
MRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSL
GVILFICLSGYPPFSEHRTQVSLKDQITSGKYNF
IPEVWAEVSEKALDLVKKLLVVDPKARFTTEEA
LRHPWLQDEDMKRKFQDLLSEENESTALPQVLA
QPSTSRKRPREGEAEGAETTKRPAVCAAVL*TPW
FEHERNVPSFTLSSFFSLSLFFYSLYFNYGNNC
FFTVTDVQLKT*WNLGFVLLLDNRFFS*MALLF
IYLRWSLTLLPSLNEVQWCRLGSLQPLLPRFKRF
LCLSLLSSWDYRYAPPCLLIFIYF

Sequence CHEK2.hAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAAGTGAGTAAAGATGAGTAAGTTGCTGGGCGTGGTGGCTCACACCTGTAATCC
CAGCACTTTGGGAGGCTGAGGCGGATGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACCCACATGGAGAAACCCTGTCTCTACTAAAAATACAAA
ATAAGCCGGGCATGGTGGTGCATGCCTGTAATCCCAGCTATTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCA
AGATCGTGCCATTGCACTCTAGCCTGGGCAACAAGAGTGAAACTCTGTCTCAAAACAAAAAAAAAGATGATTTTTGTCTTTTTTGATCTGACTGTAGATG
ATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAA
AACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATA
GAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAG
AGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAA
CGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATT
TTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTG
TGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCAC
CAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGT
TTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTC
TACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGC
TGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTT
AATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTT
GAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCT
TCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGGKFVLSLQNMV*VSKDE*VAGRGGSHL*S
QHFGRLRRMDHLRSGVRDQPDPHGETLSLLKIQ
NKPGMVVHACNPSYSGG*GRRIA*TREAEVAVSQ
DRAIAL*PGQQE*NSVSKQKKR*FLSFLI*L*M
ISQFILRH*EMNTSCQKLLEVVPVER*SWLSRG
KHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK*
KF*KS*IILASSRLKTFLMQKIIILFWN*WKGE
SCLTKWWGINA*KKLPASSIFTRCSWLCSTFMK
TVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPRF
WERPLS*EPYVEPPPTWRLKFLFLLGLLGITVL
WTAGV*ELFFLSALVGIHLSLSIGLKCH*RIRS
PVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRHV
LRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQL
YPRF*PSLLLVESGPVKGKPRVPRPQSAQLCVL
LCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCIL
IMGIIAFSQSLMYN*KPDGTWALCFCLIIGSLV
EWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNLC
FPGSSDSCASAS*VAGITGMHHHAC*FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREENLSCPYRIWFK*VKMSKLLGVVAHTCNP
STLGG*GGWIT*GQEFETSLTHMEKPCLY*KYK
ISRAWWCMPVIPAIREAEAGESLEPGRQRLQ*A
KIVPLHSSLGNKSETLSQNKKKDDFCLF*SDCR*
SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFREE
NM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NRN
RNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRGR
AV*QSGGE*TPERSYLQALFLPDALGCAVPS*K
RYYTP*LKARECFTVISRRGLSYKDY*FWALQD
FGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PCC
GLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSDH
QWKIQLHS*SLGRSLRESSGPCQEVVGSGSKGT
FYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHSS
TPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVCC
CVVNSVV*TRKKCTFFHSVIFLFFESVFL*FVF
*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*L
NGFIIYLFEMESHSVAQPE*SAVVQAWLTATSA
SQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGRKICPVLTEYGLSE*R*VSCWAWWLTPVI
PALWEAEADGSPEVRSSRPA*PTWRNPVSTKNTK
*AGHGGACL*SQLFGRLRQENRLNPGGRGCSEP
RSCHCTLAWATRVKLCLKTKKKMIFVFFDLTVD
DQSVYPKALRDEYIMSKTLGSGACGEVKLAFERK
TCKKVAIKIISKRKFAIGSAREADPALNVETEI
EILKKLNHPCIIKIKNFFDAEDYYIVLELMEGG
ELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHEN
GIIHRDLKPENVLLSSQEEDCLIKITDFGHSKI
LGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRA
VDCWSLGVILFICLSGYPPFSEHRTQVSLKDQIT
SGKYNFIPEVWAEVSEKALDLVKKLLVVDPKAR
FTTEEALRHPWLQDEDMKRKFQDLLSEENESTA
LPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAA
VL*TPWFEHERNVPSFTLSSFFSLSLFFYSLYF
NYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFFS
*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPLL
PRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

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