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Sequences

Sequence CHEK2.kAug05

CAAGTGATCCTCCCACCTCAGCTTCCCAAGTAGCTGGGACTACAGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTG
CCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAG
CCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAA
GAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGA
GGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGT
GGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTG
AATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGC
CATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGG
TGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGA
CTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAG
GAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTT
CATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCC
AGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTG
GTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGC
TTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTA
TTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATT
CTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Multiple exon skipping

Frame 0

QVILPPQLPK*LGLQVVMSRESDVEAQQSHGSS
ACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQ
EPEEPTPAPWARLWALQDGFANLECVNDNYWFG
RDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV
GPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPL
NNNSEIALSLSRNKVVPVER*SWLSRGKHVRK*
P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*
IILASSRLKTFLMQKIIILFWN*WKGESCLTKW
WGINA*KKLPASSIFTRCSWLCSTFMKTVLYTV
T*SQRMFYCHLKKRTVL*RLLILGTPRFWERPLS
*EPYVEPPPTWRLKFLFLLGLLGITVLWTAGV*
ELFFLSALVGIHLSLSIGLKCH*RIRSPVENTT
SFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQKKP
*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF*P
SLLLVESGPVKGKPRVPRPQSAQLCVLLCCELR
GLNTKEMYLLSLCHLSFL*VCFFIVCILIMGIIA
FSQSLMYN*KPDGTWALCFCLIIGSLVEWLYYL
FI*DGVSLCCPA*MKCSGAGLAHCNLCFPGSSD
SCASAS*VAGITGMHHHAC*FLYIL

Frame 1

K*SSHLSFPSSWDYRS*CLGSRMLRLSSLMAAV
PVHSPMAALPSPKAPPHSPRAYPAPLPARCQTP
ASPLTPALGH*AP*RQCPLRNSILFLRTKNLRTK
NLRSLPLPPGLDYGPFRMDLPILNV*MTTTGLG
GTKAVNIALMNHC*KEQINTEHTARNTFGFSGK
WVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL*
ITILKLHCH*AEIKWCLWRGKAGFREENM*ESS
HKDHQQKEVCYWFSKRGRPSSQC*NRNRNFEKA
KSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSG
GE*TPERSYLQALFLPDALGCAVPS*KRYYTP*
LKARECFTVISRRGLSYKDY*FWALQDFGRDLS
HENLMWNPHLLGA*SSCFCWDCWV*PCCGLLEFR
SYSFYLP*WVSTFL*A*DSSVTEGSDHQWKIQL
HS*SLGRSLRESSGPCQEVVGSGSKGTFYDRRS
LKTPVASG*RHEEKVSRSSV*GK*IHSSTPGSSP
AFY*SKAAP*RGSRGCRDHKAPSCVCCCVVNSV
V*TRKKCTFFHSVIFLFFESVFL*FVF*LWE*L
LFHSH*CTIKNLMEPGLCASA**SVL*LNGFIIY
LFEMESHSVAQPE*SAVVQAWLTATSASQVQAI
LVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

SDPPTSASQVAGTTGRDVSGVGC*GSAVSWQQC
LFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQ
PVLSLQLWDTELLRDSVHSGTLFYS*GPRT*GP
RT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVWE
GQKL*ILL**TTAEKNR*IPNIQQETLSDFQGS
GS*KLLHCIHRRSQWQWNLCKYRACRERKTPSF
E*QF*NCTVTKQK*SGACGEVKLAFERKTCKKVA
IKIISKRKFAIGSAREADPALNVETEIEILKKL
NHPCIIKIKNFFDAEDYYIVLELMEGGELFDKV
VGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRD
LKPENVLLSSQEEDCLIKITDFGHSKILGETSL
MRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSL
GVILFICLSGYPPFSEHRTQVSLKDQITSGKYNF
IPEVWAEVSEKALDLVKKLLVVDPKARFTTEEA
LRHPWLQDEDMKRKFQDLLSEENESTALPQVLA
QPSTSRKRPREGEAEGAETTKRPAVCAAVL*TPW
FEHERNVPSFTLSSFFSLSLFFYSLYFNYGNNC
FFTVTDVQLKT*WNLGFVLLLDNRFFS*MALLF
IYLRWSLTLLPSLNEVQWCRLGSLQPLLPRFKRF
LCLSLLSSWDYRYAPPCLLIFIYF

Sequence CHEK2.jAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG
AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGAGAAAATACTTAAAATCTACTCTCTCAGCTGATTTTCAAAA
ATACGGCGGGGCGCGGTGGCTCACGTCTTTAATCCCAGCACTTTGGGAGGCCGAGGCTGGCAGATCACCTGAGTTTTTGTCTTTTTTGATCTGACTGTAG
ATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAG
GAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAA
ATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGG
GAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGA
AAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAG
ATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTG
CTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGAT
CACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCA
CGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAG
CTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGC
TGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTAT
TTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTA
GTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCT
GCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Multiple exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER
KTPSFE*QF*NCTVTKQK*RENT*NLLSQLIFK
NTAGRGGSRL*SQHFGRPRLADHLSFCLF*SDCR
*SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFRE
ENM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NR
NRNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRG
RAV*QSGGE*TPERSYLQALFLPDALGCAVPS*
KRYYTP*LKARECFTVISRRGLSYKDY*FWALQ
DFGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PC
CGLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSD
HQWKIQLHS*SLGRSLRESSGPCQEVVGSGSKG
TFYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHS
STPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVC
CCVVNSVV*TRKKCTFFHSVIFLFFESVFL*FV
F*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*
LNGFIIYLFEMESHSVAQPE*SAVVQAWLTATS
ASQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG
KRRPLNNNSEIALSLSRNKEKILKIYSLS*FSK
IRRGAVAHVFNPSTLGGRGWQIT*VFVFFDLTV
DDQSVYPKALRDEYIMSKTLGSGACGEVKLAFER
KTCKKVAIKIISKRKFAIGSAREADPALNVETE
IEILKKLNHPCIIKIKNFFDAEDYYIVLELMEG
GELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHE
NGIIHRDLKPENVLLSSQEEDCLIKITDFGHSK
ILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNR
AVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQI
TSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKA
RFTTEEALRHPWLQDEDMKRKFQDLLSEENEST
ALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCA
AVL*TPWFEHERNVPSFTLSSFFSLSLFFYSLY
FNYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFF
S*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPL
LPRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK
ENAVL*ITILKLHCH*AEIKRKYLKSTLSADFQK
YGGARWLTSLIPALWEAEAGRSPEFLSFLI*L*
MISQFILRH*EMNTSCQKLLEVVPVER*SWLSR
GKHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK
*KF*KS*IILASSRLKTFLMQKIIILFWN*WKG
ESCLTKWWGINA*KKLPASSIFTRCSWLCSTFM
KTVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPR
FWERPLS*EPYVEPPPTWRLKFLFLLGLLGITV
LWTAGV*ELFFLSALVGIHLSLSIGLKCH*RIR
SPVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRH
VLRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQ
LYPRF*PSLLLVESGPVKGKPRVPRPQSAQLCV
LLCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCI
LIMGIIAFSQSLMYN*KPDGTWALCFCLIIGSL
VEWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNL
CFPGSSDSCASAS*VAGITGMHHHAC*FLYIL

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