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Sequences

Sequence CHEK2.lAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAATGGTGCCTGTGG AGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGAC CCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATA TTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCT CTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK RTDKYRTYSKKHFRIFREENLSCPYRIWFNGACG EVKLAFERKTCKKVAIKIISKRKFAIGSAREAD PALNVETEIEILKKLNHPCIIKIKNFFDAEDYY IVLELMEGGELFDKVVGNKRLKEATCKLYFYQML LAVQYLHENGIIHRDLKPENVLLSSQEEDCLIK

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K EQINTEHTARNTFGFSGRKICPVLTEYGLMVPV ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQT QLSMLKQK*KF*KS*IILASSRLKTFLMQKIII LFWN*WKGESCLTKWWGINA*KKLPASSIFTRC SWLCSTFMKTVLYTVT*SQRMFYCHLKKRTVL*

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK NR*IPNIQQETLSDFQGGKFVLSLQNMV*WCLW RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR PSSQC*NRNRNFEKAKSSLHHQD*KLF*CRRLLY CFGIDGRGRAV*QSGGE*TPERSYLQALFLPDA LGCAVPS*KRYYTP*LKARECFTVISRRGLSYK

Sequence CHEK2.hAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC GGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAAGTGAGTAAAGATGAGTAAGTTGCTGGGCGTGGTGGCTCACACCTGTAATCC CAGCACTTTGGGAGGCTGAGGCGGATGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACCCACATGGAGAAACCCTGTCTCTACTAAAAATACAAA ATAAGCCGGGCATGGTGGTGCATGCCTGTAATCCCAGCTATTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCA AGATCGTGCCATTGCACTCTAGCCTGGGCAACAAGAGTGAAACTCTGTCTCAAAACAAAAAAAAAGATGATTTTTGTCTTTTTTGATCTGACTGTAGATG ATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAA AACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATA GAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAG AGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAA CGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATT TTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTG TGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCAC CAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGT TTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTC TACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGC TGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTT AATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTT GAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCT TCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE* QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS DFQGGKFVLSLQNMV*VSKDE*VAGRGGSHL*S QHFGRLRRMDHLRSGVRDQPDPHGETLSLLKIQ NKPGMVVHACNPSYSGG*GRRIA*TREAEVAVSQ DRAIAL*PGQQE*NSVSKQKKR*FLSFLI*L*M ISQFILRH*EMNTSCQKLLEVVPVER*SWLSRG KHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK* KF*KS*IILASSRLKTFLMQKIIILFWN*WKGE SCLTKWWGINA*KKLPASSIFTRCSWLCSTFMK TVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPRF WERPLS*EPYVEPPPTWRLKFLFLLGLLGITVL WTAGV*ELFFLSALVGIHLSLSIGLKCH*RIRS PVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRHV LRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQL YPRF*PSLLLVESGPVKGKPRVPRPQSAQLCVL LCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCIL IMGIIAFSQSLMYN*KPDGTWALCFCLIIGSLV EWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNLC FPGSSDSCASAS*VAGITGMHHHAC*FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLECVND NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF RIFREENLSCPYRIWFK*VKMSKLLGVVAHTCNP STLGG*GGWIT*GQEFETSLTHMEKPCLY*KYK ISRAWWCMPVIPAIREAEAGESLEPGRQRLQ*A KIVPLHSSLGNKSETLSQNKKKDDFCLF*SDCR* SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFREE NM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NRN RNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRGR AV*QSGGE*TPERSYLQALFLPDALGCAVPS*K RYYTP*LKARECFTVISRRGLSYKDY*FWALQD FGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PCC GLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSDH QWKIQLHS*SLGRSLRESSGPCQEVVGSGSKGT FYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHSS TPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVCC CVVNSVV*TRKKCTFFHSVIFLFFESVFL*FVF *LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*L NGFIIYLFEMESHSVAQPE*SAVVQAWLTATSA SQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF GFSGRKICPVLTEYGLSE*R*VSCWAWWLTPVI PALWEAEADGSPEVRSSRPA*PTWRNPVSTKNTK *AGHGGACL*SQLFGRLRQENRLNPGGRGCSEP RSCHCTLAWATRVKLCLKTKKKMIFVFFDLTVD DQSVYPKALRDEYIMSKTLGSGACGEVKLAFERK TCKKVAIKIISKRKFAIGSAREADPALNVETEI EILKKLNHPCIIKIKNFFDAEDYYIVLELMEGG ELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHEN GIIHRDLKPENVLLSSQEEDCLIKITDFGHSKI LGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRA VDCWSLGVILFICLSGYPPFSEHRTQVSLKDQIT SGKYNFIPEVWAEVSEKALDLVKKLLVVDPKAR FTTEEALRHPWLQDEDMKRKFQDLLSEENESTA LPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAA VL*TPWFEHERNVPSFTLSSFFSLSLFFYSLYF NYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFFS *MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPLL PRFKRFLCLSLLSSWDYRYAPPCLLIFIYF