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Sequences

Sequence CHEK2.fAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC CTTCAGGATGGATTTGCCAATCTTGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGT CAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTT TGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAA AACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTA CCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTC ATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACC TACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTG GGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTC AGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAA GACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCC GTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTC TTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTA AAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGT TGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTG GGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Multiple exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLVFVFFDLTVDDQSVYPKALRDEYIM SKTLGSGACGEVKLAFERKTCKKVAIKIISKRKF AIGSAREADPALNVETEIEILKKLNHPCIIKIK NFFDAEDYYIVLELMEGGELFDKVVGNKRLKEA TCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS SQEEDCLIKITDFGHSKILGETSLMRTLCGTPT YLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS GYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVS EKALDLVKKLLVVDPKARFTTEEALRHPWLQDE DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP REGEAEGAETTKRPAVCAAVL*TPWFEHERNVPS FTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSSW DYRYAPPCLLIFIYF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILFLSFLI*L*MISQFILRH*EMNTSC QKLLEVVPVER*SWLSRGKHVRK*P*RSSAKGS LLLVQQERQTQLSMLKQK*KF*KS*IILASSRLK TFLMQKIIILFWN*WKGESCLTKWWGINA*KKL PASSIFTRCSWLCSTFMKTVLYTVT*SQRMFYC HLKKRTVL*RLLILGTPRFWERPLS*EPYVEPPP TWRLKFLFLLGLLGITVLWTAGV*ELFFLSALV GIHLSLSIGLKCH*RIRSPVENTTSFLKSGQKS QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRMK T*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN* KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA GITGMHHHAC*FLYIL

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQSCFCLF*SDCR*SVSLS*GIKR*IHHV KNSWKWCLWRGKAGFREENM*ESSHKDHQQKEV CYWFSKRGRPSSQC*NRNRNFEKAKSSLHHQD* KLF*CRRLLYCFGIDGRGRAV*QSGGE*TPERSY LQALFLPDALGCAVPS*KRYYTP*LKARECFTV ISRRGLSYKDY*FWALQDFGRDLSHENLMWNPH LLGA*SSCFCWDCWV*PCCGLLEFRSYSFYLP*W VSTFL*A*DSSVTEGSDHQWKIQLHS*SLGRSL RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG* RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAAP *RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI KNLMEPGLCASA**SVL*LNGFIIYLFEMESHSV AQPE*SAVVQAWLTATSASQVQAILVPQPPE*L GLQVCTTMPVNFYIF

Sequence CHEK2.aAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGC CATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGGTGTGAGGCACCATCCCCGGC CAGTTTGCAGTCTAAAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAA ATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACC TTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTT TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT ATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Multiple exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*DRVW PCYPI*S*TPAVI*SSCLSLPKCWDKRCEAPSPA SLQSKMCE*QLLVWEGQKL*ILL**TTAEKNR* IPNIQQETLSDFQGSGS*KLLHCIHRRSQWQWN LCKYRACRERKTPSFE*QF*NCTVTKQK*SFCLF *SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC AVPS*KRYYTP*LKARECFTVISRRGLSYKDY* FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA* *SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF YIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLETESG HVTQSDLELLLSSDPPASASQSAGIRGVRHHPR PVCSLKCVNDNYWFGRDKSCEYCFDEPLLKRTDK YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGT FVNTELVGKGKRRPLNNNSEIALSLSRNKVFVF FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK LAFERKTCKKVAIKIISKRKFAIGSAREADPAL NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF IYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILRQSLA MLPNLILNSCCHLILLPQPPKVLG*EV*GTIPG QFAV*NV*MTTTGLGGTKAVNIALMNHC*KEQI NTEHTARNTFGFSGKWVLKTLTLHT*KITVAMEP L*IQSL*GKENAVL*ITILKLHCH*AEIKFLSF LI*L*MISQFILRH*EMNTSCQKLLEVVPVER* SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW* WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL YIL