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Sequences

Sequence CHEK2.lAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT
CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA
GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAATGGTGCCTGTGG
AGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGAC
CCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATA
TTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCT
CTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS
SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK
RTDKYRTYSKKHFRIFREENLSCPYRIWFNGACG
EVKLAFERKTCKKVAIKIISKRKFAIGSAREAD
PALNVETEIEILKKLNHPCIIKIKNFFDAEDYY
IVLELMEGGELFDKVVGNKRLKEATCKLYFYQML
LAVQYLHENGIIHRDLKPENVLLSSQEEDCLIK

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP
PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K
EQINTEHTARNTFGFSGRKICPVLTEYGLMVPV
ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQT
QLSMLKQK*KF*KS*IILASSRLKTFLMQKIII
LFWN*WKGESCLTKWWGINA*KKLPASSIFTRC
SWLCSTFMKTVLYTVT*SQRMFYCHLKKRTVL*

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL
LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK
NR*IPNIQQETLSDFQGGKFVLSLQNMV*WCLW
RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR
PSSQC*NRNRNFEKAKSSLHHQD*KLF*CRRLLY
CFGIDGRGRAV*QSGGE*TPERSYLQALFLPDA
LGCAVPS*KRYYTP*LKARECFTVISRRGLSYK

Sequence CHEK2.kAug05

CAAGTGATCCTCCCACCTCAGCTTCCCAAGTAGCTGGGACTACAGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTG
CCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAG
CCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAA
GAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGA
GGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGT
GGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTG
AATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGC
CATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGG
TGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGA
CTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAG
GAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTT
CATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCC
AGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTG
GTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGC
TTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTA
TTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATT
CTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

QVILPPQLPK*LGLQVVMSRESDVEAQQSHGSS
ACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQ
EPEEPTPAPWARLWALQDGFANLECVNDNYWFG
RDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV
GPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPL
NNNSEIALSLSRNKVVPVER*SWLSRGKHVRK*
P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*
IILASSRLKTFLMQKIIILFWN*WKGESCLTKW
WGINA*KKLPASSIFTRCSWLCSTFMKTVLYTV
T*SQRMFYCHLKKRTVL*RLLILGTPRFWERPLS
*EPYVEPPPTWRLKFLFLLGLLGITVLWTAGV*
ELFFLSALVGIHLSLSIGLKCH*RIRSPVENTT
SFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQKKP
*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF*P
SLLLVESGPVKGKPRVPRPQSAQLCVLLCCELR
GLNTKEMYLLSLCHLSFL*VCFFIVCILIMGIIA
FSQSLMYN*KPDGTWALCFCLIIGSLVEWLYYL
FI*DGVSLCCPA*MKCSGAGLAHCNLCFPGSSD
SCASAS*VAGITGMHHHAC*FLYIL

Frame 1

K*SSHLSFPSSWDYRS*CLGSRMLRLSSLMAAV
PVHSPMAALPSPKAPPHSPRAYPAPLPARCQTP
ASPLTPALGH*AP*RQCPLRNSILFLRTKNLRTK
NLRSLPLPPGLDYGPFRMDLPILNV*MTTTGLG
GTKAVNIALMNHC*KEQINTEHTARNTFGFSGK
WVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL*
ITILKLHCH*AEIKWCLWRGKAGFREENM*ESS
HKDHQQKEVCYWFSKRGRPSSQC*NRNRNFEKA
KSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSG
GE*TPERSYLQALFLPDALGCAVPS*KRYYTP*
LKARECFTVISRRGLSYKDY*FWALQDFGRDLS
HENLMWNPHLLGA*SSCFCWDCWV*PCCGLLEFR
SYSFYLP*WVSTFL*A*DSSVTEGSDHQWKIQL
HS*SLGRSLRESSGPCQEVVGSGSKGTFYDRRS
LKTPVASG*RHEEKVSRSSV*GK*IHSSTPGSSP
AFY*SKAAP*RGSRGCRDHKAPSCVCCCVVNSV
V*TRKKCTFFHSVIFLFFESVFL*FVF*LWE*L
LFHSH*CTIKNLMEPGLCASA**SVL*LNGFIIY
LFEMESHSVAQPE*SAVVQAWLTATSASQVQAI
LVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

SDPPTSASQVAGTTGRDVSGVGC*GSAVSWQQC
LFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQ
PVLSLQLWDTELLRDSVHSGTLFYS*GPRT*GP
RT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVWE
GQKL*ILL**TTAEKNR*IPNIQQETLSDFQGS
GS*KLLHCIHRRSQWQWNLCKYRACRERKTPSF
E*QF*NCTVTKQK*SGACGEVKLAFERKTCKKVA
IKIISKRKFAIGSAREADPALNVETEIEILKKL
NHPCIIKIKNFFDAEDYYIVLELMEGGELFDKV
VGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRD
LKPENVLLSSQEEDCLIKITDFGHSKILGETSL
MRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSL
GVILFICLSGYPPFSEHRTQVSLKDQITSGKYNF
IPEVWAEVSEKALDLVKKLLVVDPKARFTTEEA
LRHPWLQDEDMKRKFQDLLSEENESTALPQVLA
QPSTSRKRPREGEAEGAETTKRPAVCAAVL*TPW
FEHERNVPSFTLSSFFSLSLFFYSLYFNYGNNC
FFTVTDVQLKT*WNLGFVLLLDNRFFS*MALLF
IYLRWSLTLLPSLNEVQWCRLGSLQPLLPRFKRF
LCLSLLSSWDYRYAPPCLLIFIYF

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