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Sequence CHEK2.fAug05
GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGT
CAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTT
TGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAA
AACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTA
CCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTC
ATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACC
TACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTG
GGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTC
AGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAA
GACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCC
GTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTC
TTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTA
AAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGT
TGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTG
GGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
- CHEK2.u.f.13 (forward primer)
- CHEK2.u.r.25 (reverse primer)
- Mutually exclusive exon skipping
Frame 0
VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLVFVFFDLTVDDQSVYPKALRDEYIM
SKTLGSGACGEVKLAFERKTCKKVAIKIISKRKF
AIGSAREADPALNVETEIEILKKLNHPCIIKIK
NFFDAEDYYIVLELMEGGELFDKVVGNKRLKEA
TCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS
SQEEDCLIKITDFGHSKILGETSLMRTLCGTPT
YLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS
GYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVS
EKALDLVKKLLVVDPKARFTTEEALRHPWLQDE
DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP
REGEAEGAETTKRPAVCAAVL*TPWFEHERNVPS
FTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL
KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL
LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSSW
DYRYAPPCLLIFIYF
Frame 1
S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILFLSFLI*L*MISQFILRH*EMNTSC
QKLLEVVPVER*SWLSRGKHVRK*P*RSSAKGS
LLLVQQERQTQLSMLKQK*KF*KS*IILASSRLK
TFLMQKIIILFWN*WKGESCLTKWWGINA*KKL
PASSIFTRCSWLCSTFMKTVLYTVT*SQRMFYC
HLKKRTVL*RLLILGTPRFWERPLS*EPYVEPPP
TWRLKFLFLLGLLGITVLWTAGV*ELFFLSALV
GIHLSLSIGLKCH*RIRSPVENTTSFLKSGQKS
QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRMK
T*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP
VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL
LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN*
KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC
CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA
GITGMHHHAC*FLYIL
Frame 2
RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQSCFCLF*SDCR*SVSLS*GIKR*IHHV
KNSWKWCLWRGKAGFREENM*ESSHKDHQQKEV
CYWFSKRGRPSSQC*NRNRNFEKAKSSLHHQD*
KLF*CRRLLYCFGIDGRGRAV*QSGGE*TPERSY
LQALFLPDALGCAVPS*KRYYTP*LKARECFTV
ISRRGLSYKDY*FWALQDFGRDLSHENLMWNPH
LLGA*SSCFCWDCWV*PCCGLLEFRSYSFYLP*W
VSTFL*A*DSSVTEGSDHQWKIQLHS*SLGRSL
RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG*
RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAAP
*RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF
FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI
KNLMEPGLCASA**SVL*LNGFIIYLFEMESHSV
AQPE*SAVVQAWLTATSASQVQAILVPQPPE*L
GLQVCTTMPVNFYIF
Sequence CHEK2.lAug05
GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA
GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAATGGTGCCTGTGG
AGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGAC
CCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATA
TTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCT
CTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAG
- CHEK2.u.f.13 (forward primer)
- CHEK2.u.r.25 (reverse primer)
- Mutually exclusive exon skipping
Frame 0
VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK
RTDKYRTYSKKHFRIFREENLSCPYRIWFNGACG
EVKLAFERKTCKKVAIKIISKRKFAIGSAREAD
PALNVETEIEILKKLNHPCIIKIKNFFDAEDYY
IVLELMEGGELFDKVVGNKRLKEATCKLYFYQML
LAVQYLHENGIIHRDLKPENVLLSSQEEDCLIK
Frame 1
S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K
EQINTEHTARNTFGFSGRKICPVLTEYGLMVPV
ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQT
QLSMLKQK*KF*KS*IILASSRLKTFLMQKIII
LFWN*WKGESCLTKWWGINA*KKLPASSIFTRC
SWLCSTFMKTVLYTVT*SQRMFYCHLKKRTVL*
Frame 2
RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK
NR*IPNIQQETLSDFQGGKFVLSLQNMV*WCLW
RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR
PSSQC*NRNRNFEKAKSSLHHQD*KLF*CRRLLY
CFGIDGRGRAV*QSGGE*TPERSYLQALFLPDA
LGCAVPS*KRYYTP*LKARECFTVISRRGLSYK
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