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Sequences

Sequence CHEK2.fAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT
CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGT
CAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTT
TGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAA
AACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTA
CCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTC
ATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACC
TACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTG
GGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTC
AGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAA
GACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCC
GTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTC
TTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTA
AAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGT
TGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTG
GGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Multiple exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS
SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLVFVFFDLTVDDQSVYPKALRDEYIM
SKTLGSGACGEVKLAFERKTCKKVAIKIISKRKF
AIGSAREADPALNVETEIEILKKLNHPCIIKIK
NFFDAEDYYIVLELMEGGELFDKVVGNKRLKEA
TCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS
SQEEDCLIKITDFGHSKILGETSLMRTLCGTPT
YLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS
GYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVS
EKALDLVKKLLVVDPKARFTTEEALRHPWLQDE
DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP
REGEAEGAETTKRPAVCAAVL*TPWFEHERNVPS
FTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL
KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL
LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSSW
DYRYAPPCLLIFIYF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP
PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILFLSFLI*L*MISQFILRH*EMNTSC
QKLLEVVPVER*SWLSRGKHVRK*P*RSSAKGS
LLLVQQERQTQLSMLKQK*KF*KS*IILASSRLK
TFLMQKIIILFWN*WKGESCLTKWWGINA*KKL
PASSIFTRCSWLCSTFMKTVLYTVT*SQRMFYC
HLKKRTVL*RLLILGTPRFWERPLS*EPYVEPPP
TWRLKFLFLLGLLGITVLWTAGV*ELFFLSALV
GIHLSLSIGLKCH*RIRSPVENTTSFLKSGQKS
QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRMK
T*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP
VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL
LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN*
KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC
CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA
GITGMHHHAC*FLYIL

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL
LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQSCFCLF*SDCR*SVSLS*GIKR*IHHV
KNSWKWCLWRGKAGFREENM*ESSHKDHQQKEV
CYWFSKRGRPSSQC*NRNRNFEKAKSSLHHQD*
KLF*CRRLLYCFGIDGRGRAV*QSGGE*TPERSY
LQALFLPDALGCAVPS*KRYYTP*LKARECFTV
ISRRGLSYKDY*FWALQDFGRDLSHENLMWNPH
LLGA*SSCFCWDCWV*PCCGLLEFRSYSFYLP*W
VSTFL*A*DSSVTEGSDHQWKIQLHS*SLGRSL
RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG*
RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAAP
*RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF
FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI
KNLMEPGLCASA**SVL*LNGFIIYLFEMESHSV
AQPE*SAVVQAWLTATSASQVQAILVPQPPE*L
GLQVCTTMPVNFYIF

Sequence CHEK2.jAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCA
AGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC
AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC
GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG
AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGAGAAAATACTTAAAATCTACTCTCTCAGCTGATTTTCAAAA
ATACGGCGGGGCGCGGTGGCTCACGTCTTTAATCCCAGCACTTTGGGAGGCCGAGGCTGGCAGATCACCTGAG
TTTTTGTCTTTTTTGATCTGACTGTAG
ATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAG
GAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAA
ATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGG
GAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGA
AAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAG
ATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTG
CTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGAT
CACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCA
CGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAG
CTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGC
TGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTAT
TTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTA
GTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCT
GCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Multiple exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GP
RT*GAYPCPLGSIMGPSGWICQS*MCE*
QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS
DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER
KTPSFE*QF*NCTVTKQK*RENT*NLLSQLIFK
NTAGRGGSRL*SQHFGRPRLADHLS
FCLF*SDCR
*SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFRE
ENM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NR
NRNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRG
RAV*QSGGE*TPERSYLQALFLPDALGCAVPS*
KRYYTP*LKARECFTVISRRGLSYKDY*FWALQ
DFGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PC
CGLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSD
HQWKIQLHS*SLGRSLRESSGPCQEVVGSGSKG
TFYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHS
STPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVC
CCVVNSVV*TRKKCTFFHSVIFLFFESVFL*FV
F*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL*
LNGFIIYLFEMESHSVAQPE*SAVVQAWLTATS
ASQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQ
EPEEPTPAPWARLWALQDGFANLECVND
NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF
RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG
KRRPLNNNSEIALSLSRNKEKILKIYSLS*FSK
IRRGAVAHVFNPSTLGGRGWQIT*
VFVFFDLTV
DDQSVYPKALRDEYIMSKTLGSGACGEVKLAFER
KTCKKVAIKIISKRKFAIGSAREADPALNVETE
IEILKKLNHPCIIKIKNFFDAEDYYIVLELMEG
GELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHE
NGIIHRDLKPENVLLSSQEEDCLIKITDFGHSK
ILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNR
AVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQI
TSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKA
RFTTEEALRHPWLQDEDMKRKFQDLLSEENEST
ALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCA
AVL*TPWFEHERNVPSFTLSSFFSLSLFFYSLY
FNYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFF
S*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPL
LPRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRT
KNLRSLPLPPGLDYGPFRMDLPILNV*MT
TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF
GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK
ENAVL*ITILKLHCH*AEIKRKYLKSTLSADFQK
YGGARWLTSLIPALWEAEAGRSPE
FLSFLI*L*
MISQFILRH*EMNTSCQKLLEVVPVER*SWLSR
GKHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK
*KF*KS*IILASSRLKTFLMQKIIILFWN*WKG
ESCLTKWWGINA*KKLPASSIFTRCSWLCSTFM
KTVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPR
FWERPLS*EPYVEPPPTWRLKFLFLLGLLGITV
LWTAGV*ELFFLSALVGIHLSLSIGLKCH*RIR
SPVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRH
VLRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQ
LYPRF*PSLLLVESGPVKGKPRVPRPQSAQLCV
LLCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCI
LIMGIIAFSQSLMYN*KPDGTWALCFCLIIGSL
VEWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNL
CFPGSSDSCASAS*VAGITGMHHHAC*FLYIL

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