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Sequences

Sequence CHEK2.fAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC CTTCAGGATGGATTTGCCAATCTTGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGT CAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTT TGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAA AACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTA CCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTC ATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACC TACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTG GGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTC AGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAA GACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCC GTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTC TTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTA AAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGT TGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTG GGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Multiple exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLVFVFFDLTVDDQSVYPKALRDEYIM SKTLGSGACGEVKLAFERKTCKKVAIKIISKRKF AIGSAREADPALNVETEIEILKKLNHPCIIKIK NFFDAEDYYIVLELMEGGELFDKVVGNKRLKEA TCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS SQEEDCLIKITDFGHSKILGETSLMRTLCGTPT YLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS GYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVS EKALDLVKKLLVVDPKARFTTEEALRHPWLQDE DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP REGEAEGAETTKRPAVCAAVL*TPWFEHERNVPS FTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSSW DYRYAPPCLLIFIYF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILFLSFLI*L*MISQFILRH*EMNTSC QKLLEVVPVER*SWLSRGKHVRK*P*RSSAKGS LLLVQQERQTQLSMLKQK*KF*KS*IILASSRLK TFLMQKIIILFWN*WKGESCLTKWWGINA*KKL PASSIFTRCSWLCSTFMKTVLYTVT*SQRMFYC HLKKRTVL*RLLILGTPRFWERPLS*EPYVEPPP TWRLKFLFLLGLLGITVLWTAGV*ELFFLSALV GIHLSLSIGLKCH*RIRSPVENTTSFLKSGQKS QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRMK T*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN* KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA GITGMHHHAC*FLYIL

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQSCFCLF*SDCR*SVSLS*GIKR*IHHV KNSWKWCLWRGKAGFREENM*ESSHKDHQQKEV CYWFSKRGRPSSQC*NRNRNFEKAKSSLHHQD* KLF*CRRLLYCFGIDGRGRAV*QSGGE*TPERSY LQALFLPDALGCAVPS*KRYYTP*LKARECFTV ISRRGLSYKDY*FWALQDFGRDLSHENLMWNPH LLGA*SSCFCWDCWV*PCCGLLEFRSYSFYLP*W VSTFL*A*DSSVTEGSDHQWKIQLHS*SLGRSL RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG* RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAAP *RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI KNLMEPGLCASA**SVL*LNGFIIYLFEMESHSV AQPE*SAVVQAWLTATSASQVQAILVPQPPE*L GLQVCTTMPVNFYIF

Sequence CHEK2.jAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGAGAAAATACTTAAAATCTACTCTCTCAGCTGATTTTCAAAA ATACGGCGGGGCGCGGTGGCTCACGTCTTTAATCCCAGCACTTTGGGAGGCCGAGGCTGGCAGATCACCTGAGTTTTTGTCTTTTTTGATCTGACTGTAG ATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAG GAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAA ATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGG GAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGA AAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAG ATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTG CTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGAT CACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCA CGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAG CTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGC TGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTAT TTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTA GTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCT GCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Multiple exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE* QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER KTPSFE*QF*NCTVTKQK*RENT*NLLSQLIFK NTAGRGGSRL*SQHFGRPRLADHLSFCLF*SDCR *SVSLS*GIKR*IHHVKNSWKWCLWRGKAGFRE ENM*ESSHKDHQQKEVCYWFSKRGRPSSQC*NR NRNFEKAKSSLHHQD*KLF*CRRLLYCFGIDGRG RAV*QSGGE*TPERSYLQALFLPDALGCAVPS* KRYYTP*LKARECFTVISRRGLSYKDY*FWALQ DFGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PC CGLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSD HQWKIQLHS*SLGRSLRESSGPCQEVVGSGSKG TFYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHS STPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVC CCVVNSVV*TRKKCTFFHSVIFLFFESVFL*FV F*LWE*LLFHSH*CTIKNLMEPGLCASA**SVL* LNGFIIYLFEMESHSVAQPE*SAVVQAWLTATS ASQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLECVND NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG KRRPLNNNSEIALSLSRNKEKILKIYSLS*FSK IRRGAVAHVFNPSTLGGRGWQIT*VFVFFDLTV DDQSVYPKALRDEYIMSKTLGSGACGEVKLAFER KTCKKVAIKIISKRKFAIGSAREADPALNVETE IEILKKLNHPCIIKIKNFFDAEDYYIVLELMEG GELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHE NGIIHRDLKPENVLLSSQEEDCLIKITDFGHSK ILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNR AVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQI TSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKA RFTTEEALRHPWLQDEDMKRKFQDLLSEENEST ALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCA AVL*TPWFEHERNVPSFTLSSFFSLSLFFYSLY FNYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFF S*MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPL LPRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK ENAVL*ITILKLHCH*AEIKRKYLKSTLSADFQK YGGARWLTSLIPALWEAEAGRSPEFLSFLI*L* MISQFILRH*EMNTSCQKLLEVVPVER*SWLSR GKHVRK*P*RSSAKGSLLLVQQERQTQLSMLKQK *KF*KS*IILASSRLKTFLMQKIIILFWN*WKG ESCLTKWWGINA*KKLPASSIFTRCSWLCSTFM KTVLYTVT*SQRMFYCHLKKRTVL*RLLILGTPR FWERPLS*EPYVEPPPTWRLKFLFLLGLLGITV LWTAGV*ELFFLSALVGIHLSLSIGLKCH*RIR SPVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRH VLRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQ LYPRF*PSLLLVESGPVKGKPRVPRPQSAQLCV LLCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCI LIMGIIAFSQSLMYN*KPDGTWALCFCLIIGSL VEWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNL CFPGSSDSCASAS*VAGITGMHHHAC*FLYIL