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Sequences

Sequence FN1.oAug05

GTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCAC CAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCC AGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTG TACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCA ATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCG GCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAG CCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTC ATCCTGTTGGCACTGATGAAGAACCCTTACAGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCC GTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGAT GGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAA AGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATT TGCTCCTGCACATGCTTTGGAGGCCAGCGGGTAAGACTGGATGTGCCAGGCTCCCTACAAGTTAGATAAGATAAAGGGTGGGCTCCTGCAAGGATGTGTC GTACACACAGGAGGGGCAGAGACCCTTCGGAAGTATTAAAATACCACATTTCCTGTTGGCATACAACTGCTGACATAGAGCTCTAGAGCAGCTCTATGTC TACCTTACATGCCATTCATTCTTTCTATTACTCTTAGTAGAAAGAATGAATGAATGGCATGTAGAGTACCAAAAACACAAGTCTTGAGTCATTCTTAATA GCAACACCTGTCATTTATATGATGTTAGAATCATTTTCCTAAGCTCCCTAGCATGTCAGAGATACTATTTACACTGAAAAATAGTGAAGCAGAGATACTA TTCAAATTAATTAGTGGTAAATAGAATGTGTTTCATTTCAGCCGGTTCTCCCCATCCTGGGCAGCCTGAGACCCTCCCCTCCCCTACTATTCTCAGGCTG CTTCTATTTTTCAGCAAAGTGTTAAGTGCAGTGTAGCTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

VATKYEVSVYALKDTLTSRPAQGVVTTLENVSP PRRARVTDATETTITISWRTKTETITGFQVDAVP ANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYL YTLNDNARSSPVVIDASTAIDAPSNLRFLATTP NSLLVSWQPPRARITGYIIKYEKPGSPPREVVPR PRPGVTEATITGLEPGTEYTIYVIALKNNQKSE PLIGRKKTGQEALSQTTISWAPFQDTSEYIISC HPVGTDEEPLQSTKA*TNLRMTRALTPTQFPIMP LEMSGNECLNQALNCCASA*ALEVVISDVIHLD GAMTMV*TTRLERSGTVREKMAR**AAHVLGTE KENSSVTLMRQRVMMMGRHTT*ENSGRRNISVPF APAHALEASG*DWMCQAPYKLDKIKGGLLQGCV VHTGGAETLRKY*NTTFPVGIQLLT*SSRAALC LPYMPFILSITLSRKNE*MACRVPKTQVLSHS** QHLSFI*C*NHFPKLPSMSEILFTLKNSEAEIL FKLISGK*NVFHFSRFSPSWAA*DPPLPYYSQA ASIFQQSVKCSVA

Frame 1

WPPNMK*VSMLLRTL*QADQLRELSPLWRMSAH QEGLV*QMLLRPPSPLAGEPRLRRSLASKLMPF QPMARLQSREPSSQMSEATPSQVYNQALTTRSTC TP*MTMLGAPLWSSTPPLPLMHHPTCVSWPPHP IPCWYHGSRHVPGLPATSSSMRSLGLLPEKWSL GPALVSQRLLLLAWNREPNIQFMSLP*RIIRRAS P*LEGKRQDKKLSLRQPSHGPHSRTLLSTSFHV ILLALMKNPYSQRRLEPTYG*LVL*PLHSFPLC RWR*VGTNV*IRL*TVVPVLRLWKWSFQM*FI*M VP*QWCELQDWREVGPSGRKWPDDELHMSWERK RRIQV*PS*GNVL**WEDIPRRRTVAEGISRCH LLLHMLWRPAGKTGCARLPTS*IR*RVGSCKDVS YTQEGQRPFGSIKIPHFLLAYNC*HRALEQLYV YLTCHSFFLLLLVERMNEWHVEYQKHKS*VILN SNTCHLYDVRIIFLSSLACQRYYLH*KIVKQRYY SN*LVVNRMCFISAGSPHPGQPETLPSPTILRL LLFFSKVLSAV*L

Frame 2

GHQI*SECLCS*GHFDKQTSSGSCHHSGECQPT KKGSCDRCY*DHHHH*LENQD*DDHWLPS*CRS SQWPDSNPENHQARCQKLHHHRFTTRH*LQDLP VHLE*QCSELPCGHRRLHCH*CTIQPAFPGHHTQ FLAGIMAAATCQDYRLHHQV*EAWVSSQRSGPS APPWCHRGYYYWPGTGNRIYNLCHCPEE*SEER APDWKEKDRTRSSLSDNHLMGPIPGHF*VHHFMS SCWH**RTLTVNEGLNQPTDDSCFDPYTVSHYA VGDEWERMSESGFKLLCQCLGFGSGHFRCDSSR WCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGK GEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAI CSCTCFGGQRVRLDVPGSLQVR*DKGWAPARMC RTHRRGRDPSEVLKYHISCWHTTADIEL*SSSMS TLHAIHSFYYS**KE*MNGM*STKNTSLESFLI ATPVIYMMLESFS*AP*HVRDTIYTEK**SRDT IQIN*W*IECVSFQPVLPILGSLRPSPPLLFSGC FYFSAKC*VQCS

Sequence FN1.nAug05

GTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCAC CAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCC AGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTG TACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCA ATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCG GCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAG CCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTC ATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGT CATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGGATGGTGCCATGACAATGGTGTGAACTAC AAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATG AGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCA GCGGGTAAGACTGGATGTGCCAGGCTCCCTACAAGTTAGATAAGATAAAGGGTGGGCTCCTGCAAGGATGTGTCGTACACACAGGAGGGGCAGAGACCCT TCGGAAGTATTAAAATACCACATTTCCTGTTGGCATACAACTGCTGACATAGAGCTCTAGAGCAGCTCTATGTCTACCTTACATGCCATTCATTCTTTCT ATTACTCTTAGTAGAAAGAATGAATGAATGGCATGTAGAGTACCAAAAACACAAGTCTTGAGTCATTCTTAATAGCAACACCTGTCATTTATATGATGTT AGAATCATTTTCCTAAGCTCCCTAGCATGTCAGAGATACTATTTACACTGAAAAATAGTGAAGCAGAGATACTATTCAAATTAATTAGTGGTAAATAGAA TGTGTTTCATTTCAGCCGGTTCTCCCCATCCTGGGCAGCCTGAGACCCTCCCCTCCCCTACTATTCTCAGGCTGCTTCTATTTTTCAGCAAAGTGTTAAG TGCAGTGTAGCTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

VATKYEVSVYALKDTLTSRPAQGVVTTLENVSP PRRARVTDATETTITISWRTKTETITGFQVDAVP ANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYL YTLNDNARSSPVVIDASTAIDAPSNLRFLATTP NSLLVSWQPPRARITGYIIKYEKPGSPPREVVPR PRPGVTEATITGLEPGTEYTIYVIALKNNQKSE PLIGRKKTGQEALSQTTISWAPFQDTSEYIISC HPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNV IVEALKDQQRHKVREEVVTVGNSGWCHDNGVNY KIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPH EATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQ RVRLDVPGSLQVR*DKGWAPARMCRTHRRGRDP SEVLKYHISCWHTTADIEL*SSSMSTLHAIHSF YYS**KE*MNGM*STKNTSLESFLIATPVIYMML ESFS*AP*HVRDTIYTEK**SRDTIQIN*W*IE CVSFQPVLPILGSLRPSPPLLFSGCFYFSAKC* VQCS

Frame 1

WPPNMK*VSMLLRTL*QADQLRELSPLWRMSAH QEGLV*QMLLRPPSPLAGEPRLRRSLASKLMPF QPMARLQSREPSSQMSEATPSQVYNQALTTRSTC TP*MTMLGAPLWSSTPPLPLMHHPTCVSWPPHP IPCWYHGSRHVPGLPATSSSMRSLGLLPEKWSL GPALVSQRLLLLAWNREPNIQFMSLP*RIIRRAS P*LEGKRQDKKLSLRQPSHGPHSRTLLSTSFHV ILLALMKNPYSSGFLELLPVPL*QASPEVPPTT S*WRH*KTSRGIRFGKRLLPWATLDGAMTMV*TT RLERSGTVREKMAR**AAHVLGTEKENSSVTLM RQRVMMMGRHTT*ENSGRRNISVPFAPAHALEA SG*DWMCQAPYKLDKIKGGLLQGCVVHTGGAETL RKY*NTTFPVGIQLLT*SSRAALCLPYMPFILS ITLSRKNE*MACRVPKTQVLSHS**QHLSFI*C *NHFPKLPSMSEILFTLKNSEAEILFKLISGK*N VFHFSRFSPSWAA*DPPLPYYSQAASIFQQSVK CSVA

Frame 2

GHQI*SECLCS*GHFDKQTSSGSCHHSGECQPT KKGSCDRCY*DHHHH*LENQD*DDHWLPS*CRS SQWPDSNPENHQARCQKLHHHRFTTRH*LQDLP VHLE*QCSELPCGHRRLHCH*CTIQPAFPGHHTQ FLAGIMAAATCQDYRLHHQV*EAWVSSQRSGPS APPWCHRGYYYWPGTGNRIYNLCHCPEE*SEER APDWKEKDRTRSSLSDNHLMGPIPGHF*VHHFMS SCWH**RTLTVQGSWNFYQCHSDRPHQRCHLQR HSGGTERPAEA*GSGRGCYRGQLWMVP*QWCEL QDWREVGPSGRKWPDDELHMSWERKRRIQV*PS* GNVL**WEDIPRRRTVAEGISRCHLLLHMLWRP AGKTGCARLPTS*IR*RVGSCKDVSYTQEGQRP FGSIKIPHFLLAYNC*HRALEQLYVYLTCHSFFL LLLVERMNEWHVEYQKHKS*VILNSNTCHLYDV RIIFLSSLACQRYYLH*KIVKQRYYSN*LVVNR MCFISAGSPHPGQPETLPSPTILRLLLFFSKVLS AV*L

Sequence FN1.jAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACC ATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTT CTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGT TGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAG TGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACA ATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAA GTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACA TGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATT CTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTA AATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTT CTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATG AGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCAT ATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCC GCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATC TTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTAT AATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAA GTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTA ATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTT CTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACC ATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAA AAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACT TTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGG GATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGA GACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCT GAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCA AAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGC AAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCAT GTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAG CTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAG CCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDRTRSSLSDN HLMGPIPGHF*VHHFMSSCWH**RTLTVQGSWNF YQCHSDRPHQRCHLQRHSGGTERPAEA*GSGRG CYRGQLCQRRLEPTYG*LVL*PLHSFPLCRWR* VGTNV*IRL*TVVPVLRLWKWSFQM*FI*MVP*Q WCELQDWREVGPSGRKWPDDELHMSWERKRRIQ V*PS*GNVL**WEDIPRRRTVAEGISRCHLLLH MLWRPAGLAL*QLPQTWG*TQSRRHYWPVLQPVF SEIPSENKH*C*LPN*VLHAFRCTG*QRRFPRV NHLSNPEEQACLSAKIHLNWSDVSRPSLEFFFL S*ALCSGGSSPASAQLTASPSITLGVS*GFSHK* GLHIACSASKYSIPLSILNEVILRFGLGSIGKH MQPTKMQMF*NDMTKILSRKVTQTLLLSLKCLA RNTVGTSMILLL*YFKYPQYSLFPNDPSNCLEIS FSYLLFINFSQYFYTEKIVLKTLSMQLIRGIWY NYGG*LFFILYVPKLYYCGKTTVLIKDLHSTT* SSSI*YKTPSGEIIPVNILFQFSKHLKIYDVQV* LLISVQDFLNQLLQKLIGFCHFISSLMEIALHF LL**I*VDPNIY*NC*FTVQKYNRNNL*LLFSN HCNSSLLPSTFPSLNKPLFKEIACKGNKND*DIK SI*IV*YGGVLS*GNPMV**PPSNMSYFGSADT FGMA**TPS*TVCLLLICKGHFFFNLTLRKQTL GLNLTFRPGAVAHTSNPSTLGG*GRWIT*GQEFE TSLANMVKPCLY*KYKCYQAWQWASLVPATQKA EVGEWLEPRRRRLQ*AEIVPLNSSMGDRARLHP KKHK*INVTFRIVNSGNGNNY**KISSS**TRHA KHFLNILMYNERRYFNKHLAKNIKTKYRKILPH VHLKKKNTNSHLN*FTVYKML*HLAISFRGGFR A*FYRA*SSDEVSLAKYRPSPWTSIYY*SLSFIS PIVTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTGQEALSQTT ISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGT STSATLTGLTRGATYNVIVEALKDQQRHKVREEV VTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDE WERMSESGFKLLCQCLGFGSGHFRCDSSRWCHD NGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFK CDPHEATCYDDGKTYHVGEQWQKEYLGAICSCT CFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQY SQRYHQRTNTNVNCPIECFMPLDVQADREDSRE* IIFPIQRNKHVSLPRSI*TGVMLADPA*SSSFF LKPFALEEVLQLQLNSQLLQASPWEFPEGFLIN EGCTLPVLLRSIQYRSVF*MK*F*DLVWDQ*ESI CSQPRCKCFEMI*PKF*VGKSPKHFCFHLSVWP AIL*EQA*SCYCDILNIHSTHFFQMILVIA*KY LSLTCYLSIFPSIFIRKKLY*KHLVCS**EEFGI IMVGDYFLYCMCQSFTTVERQLF**KIYIPQLE VHLFDIRHLRGK*FL*IFFFNSANI*KSMMCKS NC*FQYKIF*ISCYKN*LVFVTSSLH*WR*LYTF CFNRFKWTPIFIKIASLPFRSIIEIIFSCSFLT IVILPFFPPPFLH*INLCSKRLPAREIKMTKIL KVFE*YNMEEFYLRETPWYDNPHLTCLTLGQLTL LAWLDKLPAKQFVYS*SVKVISFSI*H*GNKLW D*I*HSGQAQWLTPLIPALWEAEAGGSPEVRSL RPAWPTW*NLVSIKNTNVTRHGSGRLWSRLLRRL R*ENGLNPEGGGCSELRLCH*TPAWGTEQDSIP KNINK*M*HSE**IVEMEIIISKKLVVHNKQDM QSTF*IY*CTMKEGISINTWQRILKLNTERYFLM FI*KKKILTPI*IDLQFIKCFDI*LSHLGEVLE LNFTGHEAQMRFP*RNTDPAPGLLFITDL*ALS AQ*SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQDKKLSLRQP SHGPHSRTLLSTSFHVILLALMKNPYSSGFLEL LPVPL*QASPEVPPTTS*WRH*KTSRGIRFGKR LLPWATLSTKA*TNLRMTRALTPTQFPIMPLEMS GNECLNQALNCCASA*ALEVVISDVIHLDGAMT MV*TTRLERSGTVREKMAR**AAHVLGTEKENS SVTLMRQRVMMMGRHTT*ENSGRRNISVPFAPAH ALEASGAGAVTTAADLGVNPVPKALLASPTTSI LRDTIREQTLMLIAQLSASCL*MYRLTEKIPES KSSFQSRGTSMSLCQDPSKLE*C*QTQLRVLLSF LSPLLWRKFSSFSSTHSFSKHHPGSFLRVFS*M RAAHCLFCFEVFNTAQYFK*SDSKIWFGINRKA YAANQDANVLK*YDQNFK*ESHPNTSAFT*VSGP QYCRNKHDLVTVIF*ISTVLTFSK*S**LPRNI FLLPVIYQFFPVFLYGKNCIENT*YAVDKRNLV *LWWVIIFYTVCAKALLLWKDNCFNKRFTFHNLK FIYLI*DTFGGNNSCEYSFSIQQTFENL*CASL IVDFSTRFSKSVATKTDWFLSLHLFTNGDSFTL SALIDLSGPQYLLKLLVYRSEV**K*SLVALF*P L*FFPSSLHLSFIE*TSVQRDCLQGK*K*LRY* KYLNSIIWRSFILGKPHGMITPI*HVLLWVS*H FWHGLINSQLNSLFTPDL*RSFLFQFNIEETNFG IKFDIQARRSGSHL*SQHFGRLRQVDHLRSGV* DQPGQHGETLSLLKIQMLPGMAVGVFGPGYSEG *GRRMA*TQKAEVAVS*DCAIELQHGGQSKTPSQ KT*INKCDIQNSK*WKWK*LLVKN**FIINKTC KALFKYTNVQ*KKVFQ*TPGKEY*N*IQKDTSS CSFEKKKY*LPFELIYSL*NALTFSYLI*GRF*S LILQGMKLR*GFLSEIQTQPLDFYLLLIFKLYQ PNSHQESCSVCFLKITESTK*R*Y*TTV

Sequence FN1.hAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGA CACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATC CGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACC ACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTG GCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGG CACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTG CGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTC CTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCT GAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACT TCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCA CCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGA AGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTT AAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGA AGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTA TGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGC TGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACA CTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAA GCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTT CTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTC GAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTT TGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCT TGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTT TTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATA CTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTT CGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCT AAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCC AATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCA CCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGG AGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGC TAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCG CAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACC TTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGA AGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACAT TCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTA CAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAAC TCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAG ATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCT GTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNHYSCTN*PEVHSGHTHKPERPVD TTQCSAHWISSAGDPQGEDRTNERNQPCS*QLI RGCIRTYGGHQI*SECLCS*GHFDKQTSSGSCH HSGECQPTKKGSCDRCY*DHHHH*LENQD*DDHW LPS*CRSSQWPDSNPENHQARCQKLHHHRFTTR H*LQDLPVHLE*QCSELPCGHRRLHCH*CTIQP AFPGHHTQFLAGIMAAATCQDYRLHHQV*EAWVS SQRSGPSAPPWCHRGYYYWPGTGNRIYNLCHCP EE*SEERAPDWKEKDRTRSSLSDNHLMGPIPGH F*VHHFMSSCWH**RTLTVQGSWNFYQCHSDRPH QRCHLQRHSGGTERPAEA*GSGRGCYRGQLCQR RLEPTYG*LVL*PLHSFPLCRWR*VGTNV*IRL *TVVPVLRLWKWSFQM*FI*MVP*QWCELQDWRE VGPSGRKWPDDELHMSWERKRRIQV*PS*GNVL **WEDIPRRRTVAEGISRCHLLLHMLWRPAGLA L*QLPQTWG*TQSRRHYWPVLQPVFSEIPSENKH *C*LPN*VLHAFRCTG*QRRFPRVNHLSNPEEQ ACLSAKIHLNWSDVSRPSLEFFFLS*ALCSGGS SPASAQLTASPSITLGVS*GFSHK*GLHIACSAS KYSIPLSILNEVILRFGLGSIGKHMQPTKMQMF *NDMTKILSRKVTQTLLLSLKCLARNTVGTSMI LLL*YFKYPQYSLFPNDPSNCLEISFSYLLFINF SQYFYTEKIVLKTLSMQLIRGIWYNYGG*LFFI LYVPKLYYCGKTTVLIKDLHSTT*SSSI*YKTP SGEIIPVNILFQFSKHLKIYDVQV*LLISVQDFL NQLLQKLIGFCHFISSLMEIALHFLL**I*VDP NIY*NC*FTVQKYNRNNL*LLFSNHCNSSLLPS TFPSLNKPLFKEIACKGNKND*DIKSI*IV*YGG VLS*GNPMV**PPSNMSYFGSADTFGMA**TPS *TVCLLLICKGHFFFNLTLRKQTLGLNLTFRPG AVAHTSNPSTLGG*GRWIT*GQEFETSLANMVKP CLY*KYKCYQAWQWASLVPATQKAEVGEWLEPR RRRLQ*AEIVPLNSSMGDRARLHPKKHK*INVT FRIVNSGNGNNY**KISSS**TRHAKHFLNILMY NERRYFNKHLAKNIKTKYRKILPHVHLKKKNTN SHLN*FTVYKML*HLAISFRGGFRA*FYRA*SS DEVSLAKYRPSPWTSIYY*SLSFISPIVTKRAAL YVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQW TPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSS VVVSGLMVATKYEVSVYALKDTLTSRPAQGVVT TLENVSPPRRARVTDATETTITISWRTKTETIT GFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPG TDYKIYLYTLNDNARSSPVVIDASTAIDAPSNL RFLATTPNSLLVSWQPPRARITGYIIKYEKPGS PPREVVPRPRPGVTEATITGLEPGTEYTIYVIAL KNNQKSEPLIGRKKTGQEALSQTTISWAPFQDT SEYIISCHPVGTDEEPLQFRVPGTSTSATLTGL TRGATYNVIVEALKDQQRHKVREEVVTVGNSVNE GLNQPTDDSCFDPYTVSHYAVGDEWERMSESGF KLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGE KWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCY DDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWR CDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTN TNVNCPIECFMPLDVQADREDSRE*IIFPIQRNK HVSLPRSI*TGVMLADPA*SSSFFLKPFALEEV LQLQLNSQLLQASPWEFPEGFLINEGCTLPVLL RSIQYRSVF*MK*F*DLVWDQ*ESICSQPRCKCF EMI*PKF*VGKSPKHFCFHLSVWPAIL*EQA*S CYCDILNIHSTHFFQMILVIA*KYLSLTCYLSI FPSIFIRKKLY*KHLVCS**EEFGIIMVGDYFLY CMCQSFTTVERQLF**KIYIPQLEVHLFDIRHL RGK*FL*IFFFNSANI*KSMMCKSNC*FQYKIF *ISCYKN*LVFVTSSLH*WR*LYTFCFNRFKWTP IFIKIASLPFRSIIEIIFSCSFLTIVILPFFPP PFLH*INLCSKRLPAREIKMTKILKVFE*YNME EFYLRETPWYDNPHLTCLTLGQLTLLAWLDKLPA KQFVYS*SVKVISFSI*H*GNKLWD*I*HSGQA QWLTPLIPALWEAEAGGSPEVRSLRPAWPTW*N LVSIKNTNVTRHGSGRLWSRLLRRLR*ENGLNPE GGGCSELRLCH*TPAWGTEQDSIPKNINK*M*H SE**IVEMEIIISKKLVVHNKQDMQSTF*IY*C TMKEGISINTWQRILKLNTERYFLMFI*KKKILT PI*IDLQFIKCFDI*LSHLGEVLELNFTGHEAQ MRFP*RNTDPAPGLLFITDL*ALSAQ*SPRELL CMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PLFLHQLT*SSLRSHPQA*APSG HHPMFSSLDIECG*PPRRRPDQ*KKSTLLLTAH PWLYQDLWWPPNMK*VSMLLRTL*QADQLRELSP LWRMSAHQEGLV*QMLLRPPSPLAGEPRLRRSL ASKLMPFQPMARLQSREPSSQMSEATPSQVYNQ ALTTRSTCTP*MTMLGAPLWSSTPPLPLMHHPTC VSWPPHPIPCWYHGSRHVPGLPATSSSMRSLGL LPEKWSLGPALVSQRLLLLAWNREPNIQFMSLP *RIIRRASP*LEGKRQDKKLSLRQPSHGPHSRTL LSTSFHVILLALMKNPYSSGFLELLPVPL*QAS PEVPPTTS*WRH*KTSRGIRFGKRLLPWATLST KA*TNLRMTRALTPTQFPIMPLEMSGNECLNQAL NCCASA*ALEVVISDVIHLDGAMTMV*TTRLER SGTVREKMAR**AAHVLGTEKENSSVTLMRQRV MMMGRHTT*ENSGRRNISVPFAPAHALEASGAGA VTTAADLGVNPVPKALLASPTTSILRDTIREQT LMLIAQLSASCL*MYRLTEKIPESKSSFQSRGT SMSLCQDPSKLE*C*QTQLRVLLSFLSPLLWRKF SSFSSTHSFSKHHPGSFLRVFS*MRAAHCLFCF EVFNTAQYFK*SDSKIWFGINRKAYAANQDANV LK*YDQNFK*ESHPNTSAFT*VSGPQYCRNKHDL VTVIF*ISTVLTFSK*S**LPRNIFLLPVIYQF FPVFLYGKNCIENT*YAVDKRNLV*LWWVIIFY TVCAKALLLWKDNCFNKRFTFHNLKFIYLI*DTF GGNNSCEYSFSIQQTFENL*CASLIVDFSTRFS KSVATKTDWFLSLHLFTNGDSFTLSALIDLSGP QYLLKLLVYRSEV**K*SLVALF*PL*FFPSSLH LSFIE*TSVQRDCLQGK*K*LRY*KYLNSIIWR SFILGKPHGMITPI*HVLLWVS*HFWHGLINSQ LNSLFTPDL*RSFLFQFNIEETNFGIKFDIQARR SGSHL*SQHFGRLRQVDHLRSGV*DQPGQHGET LSLLKIQMLPGMAVGVFGPGYSEG*GRRMA*TQ KAEVAVS*DCAIELQHGGQSKTPSQKT*INKCDI QNSK*WKWK*LLVKN**FIINKTCKALFKYTNV Q*KKVFQ*TPGKEY*N*IQKDTSSCSFEKKKY* LPFELIYSL*NALTFSYLI*GRF*SLILQGMKLR *GFLSEIQTQPLDFYLLLIFKLYQPNSHQESCS VCFLKITESTK*R*Y*TTV

Sequence FN1.iAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCACTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCA CACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGA AATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACA AGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCT GGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAG CTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCC ACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACA TCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAAC CGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCAC CCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGC GGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATG GGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGT GCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCG TGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACG AATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTG AACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACC CTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGG AGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGA TACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCT TTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCC CTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGC ACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCC AACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACT GTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTT ACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGG TGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCT ATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGA TTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTA ATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAAT TCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTT GAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCAT GGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAAT TTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCC TGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGG AGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACAT AAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACT TTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTT GAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTT TACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAG TCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNHYSCTN*PEVHSGH THKPERPVDTTQCSAHWISSAGDPQGEDRTNER NQPCS*QLIRGCIRTYGGHQI*SECLCS*GHFD KQTSSGSCHHSGECQPTKKGSCDRCY*DHHHH*L ENQD*DDHWLPS*CRSSQWPDSNPENHQARCQK LHHHRFTTRH*LQDLPVHLE*QCSELPCGHRRL HCH*CTIQPAFPGHHTQFLAGIMAAATCQDYRLH HQV*EAWVSSQRSGPSAPPWCHRGYYYWPGTGN RIYNLCHCPEE*SEERAPDWKEKDSSKDPFRHP PWV*HWKWYSASWHFWSATQCWATNDL*GTWF*A DHTAHNGHPHKA*AKTIPAECRTRSSLSDNHLM GPIPGHF*VHHFMSSCWH**RTLTVQGSWNFYQ CHSDRPHQRCHLQRHSGGTERPAEA*GSGRGCYR GQLCQRRLEPTYG*LVL*PLHSFPLCRWR*VGT NV*IRL*TVVPVLRLWKWSFQM*FI*MVP*QWC ELQDWREVGPSGRKWPDDELHMSWERKRRIQV*P S*GNVL**WEDIPRRRTVAEGISRCHLLLHMLW RPAGLAL*QLPQTWG*TQSRRHYWPVLQPVFSE IPSENKH*C*LPN*VLHAFRCTG*QRRFPRVNHL SNPEEQACLSAKIHLNWSDVSRPSLEFFFLS*A LCSGGSSPASAQLTASPSITLGVS*GFSHK*GL HIACSASKYSIPLSILNEVILRFGLGSIGKHMQP TKMQMF*NDMTKILSRKVTQTLLLSLKCLARNT VGTSMILLL*YFKYPQYSLFPNDPSNCLEISFS YLLFINFSQYFYTEKIVLKTLSMQLIRGIWYNYG G*LFFILYVPKLYYCGKTTVLIKDLHSTT*SSS I*YKTPSGEIIPVNILFQFSKHLKIYDVQV*LL ISVQDFLNQLLQKLIGFCHFISSLMEIALHFLL* *I*VDPNIY*NC*FTVQKYNRNNL*LLFSNHCN SSLLPSTFPSLNKPLFKEIACKGNKND*DIKSI *IV*YGGVLS*GNPMV**PPSNMSYFGSADTFGM A**TPS*TVCLLLICKGHFFFNLTLRKQTLGLN LTFRPGAVAHTSNPSTLGG*GRWIT*GQEFETS LANMVKPCLY*KYKCYQAWQWASLVPATQKAEVG EWLEPRRRRLQ*AEIVPLNSSMGDRARLHPKKH K*INVTFRIVNSGNGNNY**KISSS**TRHAKH FLNILMYNERRYFNKHLAKNIKTKYRKILPHVHL KKKNTNSHLN*FTVYKML*HLAISFRGGFRA*F YRA*SSDEVSLAKYRPSPWTSIYY*SLSFISPI VTKRAALYVF*KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQV TPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKE INLAPDSSSVVVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITIS WRTKTETITGFQVDAVPANGQTPIQRTIKPDVRS YTITGLQPGTDYKIYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLLVSWQPPRARITGY IIKYEKPGSPPREVVPRPRPGVTEATITGLEPGT EYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTH PGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFR RTTPPTTATPIRHRPRPYPPNVGQEALSQTTISW APFQDTSEYIISCHPVGTDEEPLQFRVPGTSTS ATLTGLTRGATYNVIVEALKDQQRHKVREEVVT VGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWER MSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGV NYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCD PHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFG GQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQR YHQRTNTNVNCPIECFMPLDVQADREDSRE*II FPIQRNKHVSLPRSI*TGVMLADPA*SSSFFLKP FALEEVLQLQLNSQLLQASPWEFPEGFLINEGC TLPVLLRSIQYRSVF*MK*F*DLVWDQ*ESICS QPRCKCFEMI*PKF*VGKSPKHFCFHLSVWPAIL *EQA*SCYCDILNIHSTHFFQMILVIA*KYLSL TCYLSIFPSIFIRKKLY*KHLVCS**EEFGIIM VGDYFLYCMCQSFTTVERQLF**KIYIPQLEVHL FDIRHLRGK*FL*IFFFNSANI*KSMMCKSNC* FQYKIF*ISCYKN*LVFVTSSLH*WR*LYTFCF NRFKWTPIFIKIASLPFRSIIEIIFSCSFLTIVI LPFFPPPFLH*INLCSKRLPAREIKMTKILKVF E*YNMEEFYLRETPWYDNPHLTCLTLGQLTLLA WLDKLPAKQFVYS*SVKVISFSI*H*GNKLWD*I *HSGQAQWLTPLIPALWEAEAGGSPEVRSLRPA WPTW*NLVSIKNTNVTRHGSGRLWSRLLRRLR* ENGLNPEGGGCSELRLCH*TPAWGTEQDSIPKNI NK*M*HSE**IVEMEIIISKKLVVHNKQDMQST F*IY*CTMKEGISINTWQRILKLNTERYFLMFI *KKKILTPI*IDLQFIKCFDI*LSHLGEVLELNF TGHEAQMRFP*RNTDPAPGLLFITDL*ALSAQ* SPRELLCMFFKNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PLFLHQLT*SSLRS HPQA*APSGHHPMFSSLDIECG*PPRRRPDQ*K KSTLLLTAHPWLYQDLWWPPNMK*VSMLLRTL*Q ADQLRELSPLWRMSAHQEGLV*QMLLRPPSPLA GEPRLRRSLASKLMPFQPMARLQSREPSSQMSE ATPSQVYNQALTTRSTCTP*MTMLGAPLWSSTPP LPLMHHPTCVSWPPHPIPCWYHGSRHVPGLPAT SSSMRSLGLLPEKWSLGPALVSQRLLLLAWNRE PNIQFMSLP*RIIRRASP*LEGKRQFKRPLSSPT LGMTLEMVFSFLALLVSNPVLGNK*SLRNMVLG GPHRPQRPPP*GIGQDHTRRM*DKKLSLRQPSH GPHSRTLLSTSFHVILLALMKNPYSSGFLELLPV PL*QASPEVPPTTS*WRH*KTSRGIRFGKRLLP WATLSTKA*TNLRMTRALTPTQFPIMPLEMSGN ECLNQALNCCASA*ALEVVISDVIHLDGAMTMV* TTRLERSGTVREKMAR**AAHVLGTEKENSSVT LMRQRVMMMGRHTT*ENSGRRNISVPFAPAHAL EASGAGAVTTAADLGVNPVPKALLASPTTSILRD TIREQTLMLIAQLSASCL*MYRLTEKIPESKSS FQSRGTSMSLCQDPSKLE*C*QTQLRVLLSFLS PLLWRKFSSFSSTHSFSKHHPGSFLRVFS*MRAA HCLFCFEVFNTAQYFK*SDSKIWFGINRKAYAA NQDANVLK*YDQNFK*ESHPNTSAFT*VSGPQY CRNKHDLVTVIF*ISTVLTFSK*S**LPRNIFLL PVIYQFFPVFLYGKNCIENT*YAVDKRNLV*LW WVIIFYTVCAKALLLWKDNCFNKRFTFHNLKFI YLI*DTFGGNNSCEYSFSIQQTFENL*CASLIVD FSTRFSKSVATKTDWFLSLHLFTNGDSFTLSAL IDLSGPQYLLKLLVYRSEV**K*SLVALF*PL* FFPSSLHLSFIE*TSVQRDCLQGK*K*LRY*KYL NSIIWRSFILGKPHGMITPI*HVLLWVS*HFWH GLINSQLNSLFTPDL*RSFLFQFNIEETNFGIK FDIQARRSGSHL*SQHFGRLRQVDHLRSGV*DQP GQHGETLSLLKIQMLPGMAVGVFGPGYSEG*GR RMA*TQKAEVAVS*DCAIELQHGGQSKTPSQKT *INKCDIQNSK*WKWK*LLVKN**FIINKTCKAL FKYTNVQ*KKVFQ*TPGKEY*N*IQKDTSSCSF EKKKY*LPFELIYSL*NALTFSYLI*GRF*SLI LQGMKLR*GFLSEIQTQPLDFYLLLIFKLYQPNS HQESCSVCFLKITESTK*R*Y*TTV

Sequence FN1.dAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAA CATTGGTCCAGACACCATGCGTGTCACCTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGAT GTTGCAGAGTTGTCAATTTCTCCTTCAGACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAAC AACATGAGAGCACACCTCTTAGAGGAAGACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCA CTGGATTGCTCCTCGAGCCACCATCACTGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGG AATTCCATCACCCTCACCAACCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAAC AATCAACAGTTTCTGATGTTCCGAGGGACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATA TTACAGGATCACTTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCT GGAGTTGATTATACCATCACTGTGTATGCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACA AACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCAC TCCCAAAAATGGACCAGGACCAACAAAAACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTT AGTGTCTATGCTCAGAATCCAAGCGGAGAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGG ATGTCGATTCCATCAAAATTGCTTGGGAAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATT CCCTGCACCTGATGGTGAAGAAGACACTGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAG AGCCAGCCCCTGATTGGAACCCAGTCCACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACAC CACCCAATGTTCAGCTCACTGGATATCGAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGT GGTTGTATCAGGACTTATGGTGGCCACCAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACT CTGGAGAATGTCAGCCCACCAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCT TCCAAGTTGATGCCGTTCCAGCCAATGGCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCAC TGACTACAAGATCTACCTGTACACCTTGAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGT TTCCTGGCCACCACACCCAATTCCTTGCTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTC CCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAA GAATAATCAGAAGAGCGAGCCCCTGATTGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAG CTTCCTGGCACTTCTGGTCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCA TAAGGCATAGGCCAAGACCATACCCGCCGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACAT CATTTCATGTCATCCTGTTGGCACTGATGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCC ACCTACAACGTCATAGTGGAGGCACTGAAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACC AACCTACGGATGACTCGTGCTTTGACCCCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTG CCAGTGCTTAGGCTTTGGAAGTGGTCATTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGT CAGGGAGAAAATGGCCAGATGATGAGCTGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGA AGACATACCACGTAGGAGAACAGTGGCAGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTG CCGCAGACCTGGGGGTGAACCCAGTCCCGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAAT TGCCCAATTGAGTGCTTCATGCCTTTAGATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTC TGCCAAGATCCATCTAAACTGGAGTGATGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCA GCTCAACTCACAGCTTCTCCAAGCATCACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAA TACCGCTCAGTATTTTAAATGAAGTGATTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATAT GACCAAAATTTTAAGTAGGAAAGTCACCCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGA TATTTTAAATATCCACAGTACTCACTTTTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATT TTTATACGGAAAAAATTGTATTGAAAACACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCC AAAGCTTTACTACTGTGGAAAGACAACTGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATA ATTCCTGTGAATATTCTTTTTCAATTCAGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGC TACAAAAACTGATTGGTTTTTGTCACTTCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTA AAATTGCTAGTTTACCGTTCAGAAGTATAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCA TTGAATAAACCTCTGTTCAAAGAGATTGCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTT AGGGAAACCCCATGGTATGATAACCCCCATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTG TTTACTCCTGATCTGTAAAGGTCATTTCTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCAC ACCTCTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATT AAAAATACAAATGTTACCAGGCATGGCAGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTT GCAGTGAGCTGAGATTGTGCCATTGAACTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTA AATAGTGGAAATGGAAATAATTATTAGTAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAA GGTATTTCAATAAACACCTGGCAAAGAATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAA TTGATTTACAGTTTATAAAATGCTTTGACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCC TTAGCGAAATACAGACCCAGCCCCTGGACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTT AAAAATCACAGAAAGTACAAAATAAAGGTGATATTAAACGACTGTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPSCSSSH*PAIHQ HWSRHHACHLGSTPIH*FNQLPGALLTCEK*GR CCRVVNFSFRQCSGLNKSPAWYRICSECLQCLRT T*EHTS*RKTENRS*FPNWH*LF*YYCQLFYCA LDCSSSHHHWLQDPPSSRALQWETSRRSGAPLS EFHHPHQPHSRHRVCGQHRCS*WQRGKSLIDWPT INSF*CSEGPGSCCCDPHQPTDQLGCSCCHSEI LQDHLRRDRRK*PCPGVHCAWEQVYSYHQRP*T WS*LYHHCVCCHWPWRQPRKQQANFH*LPNRN*Q TIPDASDRCSGQQH*CQVAAFKFPCYWLQSNHH SQKWTRTNKN*NCRSRSNRNDY*RLAAHSGVCG *CLCSESKRRESASGSDCSNQH*SP*RTGIH*CG CRFHQNCLGKPTGASFQVQGDLLEP*GWNP*AI PCT*W*RRHCRAARPQTGF*VHSQCGCLAR*YG EPAPDWNPVHSYSCTN*PEVHSGHTHKPERPVDT TQCSAHWISSAGDPQGEDRTNERNQPCS*QLIR GCIRTYGGHQI*SECLCS*GHFDKQTSSGSCHH SGECQPTKKGSCDRCY*DHHHH*LENQD*DDHWL PS*CRSSQWPDSNPENHQARCQKLHHHRFTTRH *LQDLPVHLE*QCSELPCGHRRLHCH*CTIQPA FPGHHTQFLAGIMAAATCQDYRLHHQV*EAWVSS QRSGPSAPPWCHRGYYYWPGTGNRIYNLCHCPE E*SEERAPDWKEKDSSKDPFRHPPWV*HWKWYS ASWHFWSATQCWATNDL*GTWF*ADHTAHNGHPH KA*AKTIPAECRTRSSLSDNHLMGPIPGHF*VH HFMSSCWH**RTLTVQGSWNFYQCHSDRPHQRC HLQRHSGGTERPAEA*GSGRGCYRGQLCQRRLEP TYG*LVL*PLHSFPLCRWR*VGTNV*IRL*TVV PVLRLWKWSFQM*FI*MVP*QWCELQDWREVGP SGRKWPDDELHMSWERKRRIQV*PS*GNVL**WE DIPRRRTVAEGISRCHLLLHMLWRPAGLAL*QL PQTWG*TQSRRHYWPVLQPVFSEIPSENKH*C* LPN*VLHAFRCTG*QRRFPRVNHLSNPEEQACLS AKIHLNWSDVSRPSLEFFFLS*ALCSGGSSPAS AQLTASPSITLGVS*GFSHK*GLHIACSASKYS IPLSILNEVILRFGLGSIGKHMQPTKMQMF*NDM TKILSRKVTQTLLLSLKCLARNTVGTSMILLL* YFKYPQYSLFPNDPSNCLEISFSYLLFINFSQY FYTEKIVLKTLSMQLIRGIWYNYGG*LFFILYVP KLYYCGKTTVLIKDLHSTT*SSSI*YKTPSGEI IPVNILFQFSKHLKIYDVQV*LLISVQDFLNQL LQKLIGFCHFISSLMEIALHFLL**I*VDPNIY* NC*FTVQKYNRNNL*LLFSNHCNSSLLPSTFPS LNKPLFKEIACKGNKND*DIKSI*IV*YGGVLS *GNPMV**PPSNMSYFGSADTFGMA**TPS*TVC LLLICKGHFFFNLTLRKQTLGLNLTFRPGAVAH TSNPSTLGG*GRWIT*GQEFETSLANMVKPCLY *KYKCYQAWQWASLVPATQKAEVGEWLEPRRRRL Q*AEIVPLNSSMGDRARLHPKKHK*INVTFRIV NSGNGNNY**KISSS**TRHAKHFLNILMYNER RYFNKHLAKNIKTKYRKILPHVHLKKKNTNSHLN *FTVYKML*HLAISFRGGFRA*FYRA*SSDEVS LAKYRPSPWTSIYY*SLSFISPIVTKRAALYVF *KSQKVQNKGDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTN IGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYVVSVSSVYE QHESTPLRGRQKTGLDSPTGIDFSDITANSFTVH WIAPRATITGYRIRHHPEHFSGRPREDRVPHSR NSITLTNLTPGTEYVVSIVALNGREESPLLIGQ QSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRY YRITYGETGGNSPVQEFTVPGSKSTATISGLKP GVDYTITVYAVTGRGDSPASSKPISINYRTEID KPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTT PKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVV SVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDV DVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELF PAPDGEEDTAELQGLRPGSEYTVSVVALHDDME SQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWT PPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSV VVSGLMVATKYEVSVYALKDTLTSRPAQGVVTT LENVSPPRRARVTDATETTITISWRTKTETITG FQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGT DYKIYLYTLNDNARSSPVVIDASTAIDAPSNLR FLATTPNSLLVSWQPPRARITGYIIKYEKPGSP PREVVPRPRPGVTEATITGLEPGTEYTIYVIALK NNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQ LPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATP IRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYI ISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGA TYNVIVEALKDQQRHKVREEVVTVGNSVNEGLN QPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLC QCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDR QGENGQMMSCTCLGNGKGEFKCDPHEATCYDDG KTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNC RRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVN CPIECFMPLDVQADREDSRE*IIFPIQRNKHVS LPRSI*TGVMLADPA*SSSFFLKPFALEEVLQLQ LNSQLLQASPWEFPEGFLINEGCTLPVLLRSIQ YRSVF*MK*F*DLVWDQ*ESICSQPRCKCFEMI *PKF*VGKSPKHFCFHLSVWPAIL*EQA*SCYCD ILNIHSTHFFQMILVIA*KYLSLTCYLSIFPSI FIRKKLY*KHLVCS**EEFGIIMVGDYFLYCMC QSFTTVERQLF**KIYIPQLEVHLFDIRHLRGK* FL*IFFFNSANI*KSMMCKSNC*FQYKIF*ISC YKN*LVFVTSSLH*WR*LYTFCFNRFKWTPIFI KIASLPFRSIIEIIFSCSFLTIVILPFFPPPFLH *INLCSKRLPAREIKMTKILKVFE*YNMEEFYL RETPWYDNPHLTCLTLGQLTLLAWLDKLPAKQF VYS*SVKVISFSI*H*GNKLWD*I*HSGQAQWLT PLIPALWEAEAGGSPEVRSLRPAWPTW*NLVSI KNTNVTRHGSGRLWSRLLRRLR*ENGLNPEGGG CSELRLCH*TPAWGTEQDSIPKNINK*M*HSE** IVEMEIIISKKLVVHNKQDMQSTF*IY*CTMKE GISINTWQRILKLNTERYFLMFI*KKKILTPI* IDLQFIKCFDI*LSHLGEVLELNFTGHEAQMRFP *RNTDPAPGLLFITDL*ALSAQ*SPRELLCMFF KNHRKYKIKVILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQLFLLPLTCDSP TLVQTPCVSPGLHPHPLI*PTSWCVTHL*KMRKM LQSCQFLLQTMQWS*QISCLVQNM**VSPVSTN NMRAHLLEEDRKQVLIPQLALTFLILLPTLLLC TGLLLEPPSLATGSAIIPSTSVGDLEKIGCPTLG IPSPSPTSLQAQSMWSASLLLMAERKVPY*LAN NQQFLMFRGTWKLLLRPPPAY*SAGMLLLSQ*D ITGSLTERQEEIALSRSSLCLGASLQLPSAALNL ELIIPSLCMLSLAVETAPQAASQFPLITEQKLT NHPRCK*PMFRTTALVSSGCLQVPLLLVTE*PP LPKMDQDQQKLKLQVQIKQK*LLKACSPQWSMWL VSMLRIQAERVSLWFRLQ*PTLIALKDWHSLMW MSIPSKLLGKAHRGKFPGTG*PTRALRMESMSY SLHLMVKKTLQSCKASDRVLSTQSVWLPCTMIWR ASP*LEPSPQLFLHQLT*SSLRSHPQA*APSGH HPMFSSLDIECG*PPRRRPDQ*KKSTLLLTAHP WLYQDLWWPPNMK*VSMLLRTL*QADQLRELSPL WRMSAHQEGLV*QMLLRPPSPLAGEPRLRRSLA SKLMPFQPMARLQSREPSSQMSEATPSQVYNQA LTTRSTCTP*MTMLGAPLWSSTPPLPLMHHPTCV SWPPHPIPCWYHGSRHVPGLPATSSSMRSLGLL PEKWSLGPALVSQRLLLLAWNREPNIQFMSLP* RIIRRASP*LEGKRQFKRPLSSPTLGMTLEMVFS FLALLVSNPVLGNK*SLRNMVLGGPHRPQRPPP *GIGQDHTRRM*DKKLSLRQPSHGPHSRTLLST SFHVILLALMKNPYSSGFLELLPVPL*QASPEVP PTTS*WRH*KTSRGIRFGKRLLPWATLSTKA*T NLRMTRALTPTQFPIMPLEMSGNECLNQALNCC ASA*ALEVVISDVIHLDGAMTMV*TTRLERSGTV REKMAR**AAHVLGTEKENSSVTLMRQRVMMMG RHTT*ENSGRRNISVPFAPAHALEASGAGAVTT AADLGVNPVPKALLASPTTSILRDTIREQTLMLI AQLSASCL*MYRLTEKIPESKSSFQSRGTSMSL CQDPSKLE*C*QTQLRVLLSFLSPLLWRKFSSF SSTHSFSKHHPGSFLRVFS*MRAAHCLFCFEVFN TAQYFK*SDSKIWFGINRKAYAANQDANVLK*Y DQNFK*ESHPNTSAFT*VSGPQYCRNKHDLVTV IF*ISTVLTFSK*S**LPRNIFLLPVIYQFFPVF LYGKNCIENT*YAVDKRNLV*LWWVIIFYTVCA KALLLWKDNCFNKRFTFHNLKFIYLI*DTFGGN NSCEYSFSIQQTFENL*CASLIVDFSTRFSKSVA TKTDWFLSLHLFTNGDSFTLSALIDLSGPQYLL KLLVYRSEV**K*SLVALF*PL*FFPSSLHLSF IE*TSVQRDCLQGK*K*LRY*KYLNSIIWRSFIL GKPHGMITPI*HVLLWVS*HFWHGLINSQLNSL FTPDL*RSFLFQFNIEETNFGIKFDIQARRSGS HL*SQHFGRLRQVDHLRSGV*DQPGQHGETLSLL KIQMLPGMAVGVFGPGYSEG*GRRMA*TQKAEV AVS*DCAIELQHGGQSKTPSQKT*INKCDIQNS K*WKWK*LLVKN**FIINKTCKALFKYTNVQ*KK VFQ*TPGKEY*N*IQKDTSSCSFEKKKY*LPFE LIYSL*NALTFSYLI*GRF*SLILQGMKLR*GF LSEIQTQPLDFYLLLIFKLYQPNSHQESCSVCFL KITESTK*R*Y*TTV

Sequence FN1.cAug05

CGGGGCGGGGACAGCCCGGCGGGTCTCTCCTCCCCCGCGCCCCGGGCCTCCAGAGGGGCGGGAGGGGACCGTCCCATATAAGCCCCGGCTCCCGGCGCTC GGACGCCCGCGCCGGCTGTGCTGCACAGGGGGAGGAGAGGGAACCCCAGGCGCGAGCGGGAAGAGGGGACCTGCAGCCACAACTTCTCTGGTCCTCTGCA TCCCTTCTGTCCCTCCACCCGTCCCCTTCCCCACCCTCTGGCCCCCACCTTCTTGGAGGCGACAACCCCCGGGAGGCATTAGAAGGGATTTTTCCCGCAG GTTGCGAAGGGAAGCAAACTTGGTGGCAACTTGCCTCCCGGTGCGGGCGTCTCTCCCCCACCGTCTCAACATGCTTAGGGGTCCGGGGCCCGGGCTGCTG CTGCTGGCCGTCCAGTGCCTGGGGACAGCGGTGCCCTCCACGGGAGCCTCGAAGAGCAAGAGGCAGGCTCAGCAAATGGTTCAGCCCCAGTCCCCGGTGG CTGTCAGTCAAAGCAAGCCCGGTTGTTATGACAATGGAAAACACTATCAGATAAATCAACAGTGGGAGCGGACCTACCTAGGCAATGCGTTGGTTTGTAC TTGTTATGGAGGAAGCCGAGGTTTTAACTGCGAGAGTAAACCTGAAGCTGAAGAGACTTGCTTTGACAAGTACACTGGGAACACTTACCGAGTGGGTGAC ACTTATGAGCGTCCTAAAGACTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGCGAGGGAGAATAAGCTGTACCATCGCAAACCGCTGCCATGAAG GGGGTCAGTCCTACAAGATTGGTGACACCTGGAGGAGACCACATGAGACTGGTGGTTACATGTTAGAGTGTGTGTGTCTTGGTAATGGAAAAGGAGAATG GACCTGCAAGCCCATAGCTGAGAAGTGTTTTGATCATGCTGCTGGGACTTCCTATGTGGTCGGAGAAACGTGGGAGAAGCCCTACCAAGGCTGGATGATG GTAGATTGTACTTGCCTGGGAGAAGGCAGCGGACGCATCACTTGCACTTCTAGAAATAGATGCAACGATCAGGACACAAGGACATCCTATAGAATTGGAG ACACCTGGAGCAAGAAGGATAATCGAGGAAACCTGCTCCAGTGCATCTGCACAGGCAACGGCCGAGGAGAGTGGAAGTGTGAGAGGCACACCTCTGTGCA GACCACATCGAGCGGATCTGGCCCCTTCACCGATGTTCGTGCAGCTGTTTACCAACCGCAGCCTCACCCCCAGCCTCCTCCCTATGGCCACTGTGTCACA GACAGTGGTGTGGTCTACTCTGTGGGGATGCAGTGGCTGAAGACACAAGGAAATAAGCAAATGCTTTGCACGTGCCTGGGCAACGGAGTCAGCTGCCAAG AGACAGCTGTAACCCAGACTTACGGTGGCAACTCAAATGGAGAGCCATGTGTCTTACCATTCACCTACAATGGCAGGACGTTCTACTCCTGCACCACAGA AGGGCGACAGGACGGACATCTTTGGTGCAGCACAACTTCGAATTATGAGCAGGACCAGAAATACTCTTTCTGCACAGACCACACTGTTTTGGTTCAGACT CGAGGAGGAAATTCCAATGGTGCCTTGTGCCACTTCCCCTTCCTATACAACAACCACAATTACACTGATTGCACTTCTGAGGGCAGAAGAGACAACATGA AGTGGTGTGGGACCACACAGAACTATGATGCCGACCAGAAGTTTGGGTTCTGCCCCATGGCTGCCCACGAGGAAATCTGCACAACCAATGAAGGGGTCAT GTACCGCATTGGAGATCAGTGGGATAAGCAGCATGACATGGGTCACATGATGAGGTGCACGTGTGTTGGGAATGGTCGTGGGGAATGGACATGCATTGCC TACTCGCAGCTTCGAGATCAGTGCATTGTTGATGACATCACTTACAATGTGAACGACACATTCCACAAGCGTCATGAAGAGGGGCACATGCTGAACTGTA CATGCTTCGGTCAGGGTCGGGGCAGGTGGAAGTGTGATCCCGTCGACCAATGCCAGGATTCAGAGACTGGGACGTTTTATCAAATTGGAGATTCATGGGA GAAGTATGTGCATGGTGTCAGATACCAGTGCTACTGCTATGGCCGTGGCATTGGGGAGTGGCATTGCCAACCTTTACAGACCTATCCAAGCTCAAGTGGT CCTGTCGAAGTATTTATCACTGAGACTCCGAGTCAGCCCAACTCCCACCCCATCCAGTGGAATGCACCACAGCCATCTCACATTTCCAAGTACATTCTCA GGTGGAGACCTAAAAATTCTGTAGGCCGTTGGAAGGAAGCTACCATACCAGGCCACTTAAACTCCTACACCATCAAAGGCCTGAAGCCTGGTGTGGTATA CGAGGGCCAGCTCATCAGCATCCAGCAGTACGGCCACCAAGAAGTGACTCGCTTTGACTTCACCACCACCAGCACCAGCACACCTGTGACCAGCAACACC GTGACAGGAGAGACGACTCCCTTTTCTCCTCTTGTGGCCACTTCTGAATCTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCTT CCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAGGAGGGAGATGAGCCACAGTACCTGGATCTTCCAAGCACAGCCACTTCTGTGAACAT CCCTGACCTGCTTCCTGGCCGAAAATACATTGTAAATGTCTATCAGATATCTGAGGATGGGGAGCAGAGTTTGATCCTGTCTACTTCACAAACAACAGCG CCTGATGCCCCTCCTGACACGACTGTGGACCAAGTTGATGACACCTCAATTGTTGTTCGCTGGAGCAGACCCCAGGCTCCCATCACAGGGTACAGAATAG TCTATTCGCCATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACTCCGTCACCCTCAGTGACTTGCAACCTGGTGTTCAGTATAA CATCACTATCTATGCTGTGGAAGAAAATCAAGAAAGTACACCTGTTGTCATTCAACAAGAAACCACTGGCACCCCACGCTCAGATACAGTGCCCTCTCCC AGGGACCTGCAGTTTGTGGAAGTGACAGACGTGAAGGTCACCATCATGTGGACACCGCCTGAGAGTGCAGTGACCGGCTACCGTGTGGATGTGATCCCCG TCAACCTGCCTGGCGAGCACGGGCAGAGGCTGCCCATCAGCAGGAACACCTTTGCAGAAGTCACCGGGCTGTCCCCTGGGGTCACCTATTACTTCAAAGT CTTTGCAGTGAGCCATGGGAGGGAGAGCAAGCCTCTGACTGCTCAACAGACAACCAAACTGGATGCTCCCACTAACCTCCAGTTTGTCAATGAAACTGAT TCTACTGTCCTGGTGAGATGGACTCCACCTCGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGGACAGCCCAGGCAGTACAATG TGGGTCCCTCTGTCTCCAAGTACCCACTGAGGAATCTGCAGCCTGCATCTGAGTACACCGTATCCCTCGTGGCCATAAAGGGCAACCAAGAGAGCCCCAA AGCCACTGGAGTCTTTACCACACTGCAGCCTGGGAGCTCTATTCCACCTTACAACACCGAGGTGACTGAGACCACCATTGTGATCACATGGACGCCTGCT CCAAGAATTGGTTTTAAGCTGGGTGTACGACCAAGCCAGGGAGGAGAGGCACCACGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCCGGCTTGA CTCCAGGAGTAGAATACGTCTACACCATCCAAGTCCTGAGAGATGGACAGGAAAGAGATGCGCCAATTGTAAACAAAGTGGTGACACCATTGTCTCCACC AACAAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAGACATTACTGGTTATAGAATTACCACA ACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCCATGCTGATCAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACA ATGTCAGTGTTTACACTGTCAAGGATGACAAGGAAAGTGTCCCTATCTCTGATACCATCATCCCAGAGGTGCCCCAACTCACTGACCTAAGCTTTGTTGA TATAACCGATTCAAGCATCGGCCTGAGGTGGACCCCGCTAAACTCTTCCACCATTATTGGGTACCGCATCACAGTAGTTGCGGCAGGAGAAGGTATCCCT ATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTCACAGGGCTGGAGCCGGGCATTGACTATGATATCAGCGTTATCACTCTCATTAATG GCGGCGAGAGTGCCCCTACTACACTGACACAACAAACGGCTGTTCCTCCTCCCACTGACCTGCGATTCACCAACATTGGTCCAGACACCATGCGTGTCAC CTGGGCTCCACCCCCATCCATTGATTTAACCAACTTCCTGGTGCGTTACTCACCTGTGAAAAATGAGGAAGATGTTGCAGAGTTGTCAATTTCTCCTTCA GACAATGCAGTGGTCTTAACAAATCTCCTGCCTGGTACAGAATATGTAGTGAGTGTCTCCAGTGTCTACGAACAACATGAGAGCACACCTCTTAGAGGAA GACAGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACTGCCAACTCTTTTACTGTGCACTGGATTGCTCCTCGAGCCACCATCAC TGGCTACAGGATCCGCCATCATCCCGAGCACTTCAGTGGGAGACCTCGAGAAGATCGGGTGCCCCACTCTCGGAATTCCATCACCCTCACCAACCTCACT CCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCAGAGAGGAAAGTCCCTTATTGATTGGCCAACAATCAACAGTTTCTGATGTTCCGAGGG ACCTGGAAGTTGTTGCTGCGACCCCCACCAGCCTACTGATCAGCTGGGATGCTCCTGCTGTCACAGTGAGATATTACAGGATCACTTACGGAGAGACAGG AGGAAATAGCCCTGTCCAGGAGTTCACTGTGCCTGGGAGCAAGTCTACAGCTACCATCAGCGGCCTTAAACCTGGAGTTGATTATACCATCACTGTGTAT GCTGTCACTGGCCGTGGAGACAGCCCCGCAAGCAGCAAGCCAATTTCCATTAATTACCGAACAGAAATTGACAAACCATCCCAGATGCAAGTGACCGATG TTCAGGACAACAGCATTAGTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACCACCACTCCCAAAAATGGACCAGGACCAACAAA AACTAAAACTGCAGGTCCAGATCAAACAGAAATGACTATTGAAGGCTTGCAGCCCACAGTGGAGTATGTGGTTAGTGTCTATGCTCAGAATCCAAGCGGA GAGAGTCAGCCTCTGGTTCAGACTGCAGTAACCAACATTGATCGCCCTAAAGGACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGG AAAGCCCACAGGGGCAAGTTTCCAGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCATGAGCTATTCCCTGCACCTGATGGTGAAGAAGACAC TGCAGAGCTGCAAGGCCTCAGACCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGATGATATGGAGAGCCAGCCCCTGATTGGAACCCAGTCC ACAGCTATTCCTGCACCAACTGACCTGAAGTTCACTCAGGTCACACCCACAAGCCTGAGCGCCCAGTGGACACCACCCAATGTTCAGCTCACTGGATATC GAGTGCGGGTGACCCCCAAGGAGAAGACCGGACCAATGAAAGAAATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTTATGGTGGCCAC CAAATATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCTCAGGGAGTTGTCACCACTCTGGAGAATGTCAGCCCACCAAGAAGG GCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGCTGGAGAACCAAGACTGAGACGATCACTGGCTTCCAAGTTGATGCCGTTCCAGCCAATG GCCAGACTCCAATCCAGAGAACCATCAAGCCAGATGTCAGAAGCTACACCATCACAGGTTTACAACCAGGCACTGACTACAAGATCTACCTGTACACCTT GAATGACAATGCTCGGAGCTCCCCTGTGGTCATCGACGCCTCCACTGCCATTGATGCACCATCCAACCTGCGTTTCCTGGCCACCACACCCAATTCCTTG CTGGTATCATGGCAGCCGCCACGTGCCAGGATTACCGGCTACATCATCAAGTATGAGAAGCCTGGGTCTCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCC CTGGTGTCACAGAGGCTACTATTACTGGCCTGGAACCGGGAACCGAATATACAATTTATGTCATTGCCCTGAAGAATAATCAGAAGAGCGAGCCCCTGAT TGGAAGGAAAAAGACAGTTCAAAAGACCCCTTTCGTCACCCACCCTGGGTATGACACTGGAAATGGTATTCAGCTTCCTGGCACTTCTGGTCAGCAACCC AGTGTTGGGCAACAAATGATCTTTGAGGAACATGGTTTTAGGCGGACCACACCGCCCACAACGGCCACCCCCATAAGGCATAGGCCAAGACCATACCCGC CGAATGTAGGACAAGAAGCTCTCTCTCAGACAACCATCTCATGGGCCCCATTCCAGGACACTTCTGAGTACATCATTTCATGTCATCCTGTTGGCACTGA TGAAGAACCCTTACAGTTCAGGGTTCCTGGAACTTCTACCAGTGCCACTCTGACAGGCCTCACCAGAGGTGCCACCTACAACGTCATAGTGGAGGCACTG AAAGACCAGCAGAGGCATAAGGTTCGGGAAGAGGTTGTTACCGTGGGCAACTCTGTCAACGAAGGCTTGAACCAACCTACGGATGACTCGTGCTTTGACC CCTACACAGTTTCCCATTATGCCGTTGGAGATGAGTGGGAACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTGGAAGTGGTCA TTTCAGATGTGATTCATCTAGATGGTGCCATGACAATGGTGTGAACTACAAGATTGGAGAGAAGTGGGACCGTCAGGGAGAAAATGGCCAGATGATGAGC TGCACATGTCTTGGGAACGGAAAAGGAGAATTCAAGTGTGACCCTCATGAGGCAACGTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGC AGAAGGAATATCTCGGTGCCATTTGCTCCTGCACATGCTTTGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGGGGGTGAACCCAGTCC CGAAGGCACTACTGGCCAGTCCTACAACCAGTATTCTCAGAGATACCATCAGAGAACAAACACTAATGTTAATTGCCCAATTGAGTGCTTCATGCCTTTA GATGTACAGGCTGACAGAGAAGATTCCCGAGAGTAAATCATCTTTCCAATCCAGAGGAACAAGCATGTCTCTCTGCCAAGATCCATCTAAACTGGAGTGA TGTTAGCAGACCCAGCTTAGAGTTCTTCTTTCTTTCTTAAGCCCTTTGCTCTGGAGGAAGTTCTCCAGCTTCAGCTCAACTCACAGCTTCTCCAAGCATC ACCCTGGGAGTTTCCTGAGGGTTTTCTCATAAATGAGGGCTGCACATTGCCTGTTCTGCTTCGAAGTATTCAATACCGCTCAGTATTTTAAATGAAGTGA TTCTAAGATTTGGTTTGGGATCAATAGGAAAGCATATGCAGCCAACCAAGATGCAAATGTTTTGAAATGATATGACCAAAATTTTAAGTAGGAAAGTCAC CCAAACACTTCTGCTTTCACTTAAGTGTCTGGCCCGCAATACTGTAGGAACAAGCATGATCTTGTTACTGTGATATTTTAAATATCCACAGTACTCACTT TTTCCAAATGATCCTAGTAATTGCCTAGAAATATCTTTCTCTTACCTGTTATTTATCAATTTTTCCCAGTATTTTTATACGGAAAAAATTGTATTGAAAA CACTTAGTATGCAGTTGATAAGAGGAATTTGGTATAATTATGGTGGGTGATTATTTTTTATACTGTATGTGCCAAAGCTTTACTACTGTGGAAAGACAAC TGTTTTAATAAAAGATTTACATTCCACAACTTGAAGTTCATCTATTTGATATAAGACACCTTCGGGGGAAATAATTCCTGTGAATATTCTTTTTCAATTC AGCAAACATTTGAAAATCTATGATGTGCAAGTCTAATTGTTGATTTCAGTACAAGATTTTCTAAATCAGTTGCTACAAAAACTGATTGGTTTTTGTCACT TCATCTCTTCACTAATGGAGATAGCTTTACACTTTCTGCTTTAATAGATTTAAGTGGACCCCAATATTTATTAAAATTGCTAGTTTACCGTTCAGAAGTA TAATAGAAATAATCTTTAGTTGCTCTTTTCTAACCATTGTAATTCTTCCCTTCTTCCCTCCACCTTTCCTTCATTGAATAAACCTCTGTTCAAAGAGATT GCCTGCAAGGGAAATAAAAATGACTAAGATATTAAAAGTATTTGAATAGTATAATATGGAGGAGTTTTATCTTAGGGAAACCCCATGGTATGATAACCCC CATCTAACATGTCTTACTTTGGGTCAGCTGACACTTTTGGCATGGCTTGATAAACTCCCAGCTAAACAGTTTGTTTACTCCTGATCTGTAAAGGTCATTT CTTTTTCAATTTAACATTGAGGAAACAAACTTTGGGATTAAATTTGACATTCAGGCCAGGCGCAGTGGCTCACACCTCTAATCCCAGCACTTTGGGAGGC TGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTATTAAAAATACAAATGTTACCAGGCATGGC AGTGGGCGTCTTTGGTCCCGGCTACTCAGAAGGCTGAGGTAGGAGAATGGCTTGAACCCAGAAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCATTGAA CTCCAGCATGGGGGACAGAGCAAGACTCCATCCCAAAAAACATAAATAAATAAATGTGACATTCAGAATAGTAAATAGTGGAAATGGAAATAATTATTAG TAAAAAATTAGTAGTTCATAATAAACAAGACATGCAAAGCACTTTTTAAATATACTAATGTACAATGAAAGAAGGTATTTCAATAAACACCTGGCAAAGA ATATTAAAACTAAATACAGAAAGATACTTCCTCATGTTCATTTGAAAAAAAAAAATACTAACTCCCATTTGAATTGATTTACAGTTTATAAAATGCTTTG ACATTTAGCTATCTCATTTAGGGGAGGTTTTAGAGCTTAATTTTACAGGGCATGAAGCTCAGATGAGGTTTCCTTAGCGAAATACAGACCCAGCCCCTGG ACTTCTATTTATTACTGATCTTTAAGCTTTATCAGCCCAATAGTCACCAAGAGAGCTGCTCTGTATGTTTTTTAAAAATCACAGAAAGTACAAAATAAAG GTGATATTAAACGACTGTC

FN1.u.f.54 (forward primer)
FN1.u.r.64 (reverse primer)
Single exon skipping

Frame 0

RGGDSPAGLSSPAPRASRGAGGDRPI*APAPGA RTPAPAVLHRGRRGNPRREREEGTCSHNFSGPLH PFCPSTRPLPHPLAPTFLEATTPGRH*KGFFPQ VAKGSKLGGNLPPGAGVSPPPSQHA*GSGARAA AAGRPVPGDSGALHGSLEEQEAGSANGSAPVPGG CQSKQARLL*QWKTLSDKSTVGADLPRQCVGLY LLWRKPRF*LRE*T*S*RDLL*QVHWEHLPSG* HL*AS*RLHDLGLYLHRGWARENKLYHRKPLP*R GSVLQDW*HLEETT*DWWLHVRVCVSW*WKRRM DLQAHS*EVF*SCCWDFLCGRRNVGEALPRLDD GRLYLPGRRQRTHHLHF*K*MQRSGHKDIL*NWR HLEQEG*SRKPAPVHLHRQRPRRVEV*EAHLCA DHIERIWPLHRCSCSCLPTAASPPASSLWPLCH RQWCGLLCGDAVAEDTRK*ANALHVPGQRSQLPR DSCNPDLRWQLKWRAMCLTIHLQWQDVLLLHHR RATGRTSLVQHNFEL*AGPEILFLHRPHCFGSD SRRKFQWCLVPLPLPIQQPQLH*LHF*GQKRQHE VVWDHTEL*CRPEVWVLPHGCPRGNLHNQ*RGH VPHWRSVG*AA*HGSHDEVHVCWEWSWGMDMHC LLAASRSVHC**HHLQCERHIPQAS*RGAHAELY MLRSGSGQVEV*SRRPMPGFRDWDVLSNWRFMG EVCAWCQIPVLLLWPWHWGVALPTFTDLSKLKW SCRSIYH*DSESAQLPPHPVECTTAISHFQVHSQ VET*KFCRPLEGSYHTRPLKLLHHQRPEAWCGI RGPAHQHPAVRPPRSDSL*LHHHQHQHTCDQQH RDRRDDSLFSSCGHF*ICDRNHSQ*LCGLLGLSF RHRVGIPGGI*AE*GGR*ATVPGSSKHSHFCEH P*PASWPKIHCKCLSDI*GWGAEFDPVYFTNNS A*CPS*HDCGPS**HLNCCSLEQTPGSHHRVQNS LFAISRR*QHRTQPS*NCKLRHPQ*LATWCSV* HHYLCCGRKSRKYTCCHSTRNHWHPTLRYSALS QGPAVCGSDRREGHHHVDTA*ECSDRLPCGCDPR QPAWRARAEAAHQQEHLCRSHRAVPWGHLLLQS LCSEPWEGEQASDCSTDNQTGCSH*PPVCQ*N* FYCPGEMDSTSGPDNRIPTDRGPYPKRTAQAVQC GSLCLQVPTEESAACI*VHRIPRGHKGQPREPQ SHWSLYHTAAWELYSTLQHRGD*DHHCDHMDAC SKNWF*AGCTTKPGRRGTTRSDFRLRKHRCVRLD SRSRIRLHHPSPERWTGKRCANCKQSGDTIVST NKLASGGKP*HWSAHSLLGEEHHPRHYWL*NYH NPYKRPAGKFFGRSGPC*SELLHF**PESRPGVQ CQCLHCQG*QGKCPYL*YHHPRGAPTH*PKLC* YNRFKHRPEVDPAKLFHHYWVPHHSSCGRRRYP YF*RFCGLLSRILHSHRAGAGH*L*YQRYHSH*W RRECPYYTDTTNGCSSSH*PAIHQHWSRHHACH LGSTPIH*FNQLPGALLTCEK*GRCCRVVNFSF RQCSGLNKSPAWYRICSECLQCLRTT*EHTS*RK TENRS*FPNWH*LF*YYCQLFYCALDCSSSHHH WLQDPPSSRALQWETSRRSGAPLSEFHHPHQPH SRHRVCGQHRCS*WQRGKSLIDWPTINSF*CSEG PGSCCCDPHQPTDQLGCSCCHSEILQDHLRRDR RK*PCPGVHCAWEQVYSYHQRP*TWS*LYHHCV CCHWPWRQPRKQQANFH*LPNRN*QTIPDASDRC SGQQH*CQVAAFKFPCYWLQSNHHSQKWTRTNK N*NCRSRSNRNDY*RLAAHSGVCG*CLCSESKR RESASGSDCSNQH*SP*RTGIH*CGCRFHQNCLG KPTGASFQVQGDLLEP*GWNP*AIPCT*W*RRH CRAARPQTGF*VHSQCGCLAR*YGEPAPDWNPV HSYSCTN*PEVHSGHTHKPERPVDTTQCSAHWIS SAGDPQGEDRTNERNQPCS*QLIRGCIRTYGGH QI*SECLCS*GHFDKQTSSGSCHHSGECQPTKK GSCDRCY*DHHHH*LENQD*DDHWLPS*CRSSQW PDSNPENHQARCQKLHHHRFTTRH*LQDLPVHL E*QCSELPCGHRRLHCH*CTIQPAFPGHHTQFL AGIMAAATCQDYRLHHQV*EAWVSSQRSGPSAPP WCHRGYYYWPGTGNRIYNLCHCPEE*SEERAPD WKEKDSSKDPFRHPPWV*HWKWYSASWHFWSAT QCWATNDL*GTWF*ADHTAHNGHPHKA*AKTIPA ECRTRSSLSDNHLMGPIPGHF*VHHFMSSCWH* *RTLTVQGSWNFYQCHSDRPHQRCHLQRHSGGT ERPAEA*GSGRGCYRGQLCQRRLEPTYG*LVL*P LHSFPLCRWR*VGTNV*IRL*TVVPVLRLWKWS FQM*FI*MVP*QWCELQDWREVGPSGRKWPDDE LHMSWERKRRIQV*PS*GNVL**WEDIPRRRTVA EGISRCHLLLHMLWRPAGLAL*QLPQTWG*TQS RRHYWPVLQPVFSEIPSENKH*C*LPN*VLHAF RCTG*QRRFPRVNHLSNPEEQACLSAKIHLNWSD VSRPSLEFFFLS*ALCSGGSSPASAQLTASPSI TLGVS*GFSHK*GLHIACSASKYSIPLSILNEV ILRFGLGSIGKHMQPTKMQMF*NDMTKILSRKVT QTLLLSLKCLARNTVGTSMILLL*YFKYPQYSL FPNDPSNCLEISFSYLLFINFSQYFYTEKIVLK TLSMQLIRGIWYNYGG*LFFILYVPKLYYCGKTT VLIKDLHSTT*SSSI*YKTPSGEIIPVNILFQF SKHLKIYDVQV*LLISVQDFLNQLLQKLIGFCH FISSLMEIALHFLL**I*VDPNIY*NC*FTVQKY NRNNL*LLFSNHCNSSLLPSTFPSLNKPLFKEI ACKGNKND*DIKSI*IV*YGGVLS*GNPMV**P PSNMSYFGSADTFGMA**TPS*TVCLLLICKGHF FFNLTLRKQTLGLNLTFRPGAVAHTSNPSTLGG *GRWIT*GQEFETSLANMVKPCLY*KYKCYQAW QWASLVPATQKAEVGEWLEPRRRRLQ*AEIVPLN SSMGDRARLHPKKHK*INVTFRIVNSGNGNNY* *KISSS**TRHAKHFLNILMYNERRYFNKHLAK NIKTKYRKILPHVHLKKKNTNSHLN*FTVYKML* HLAISFRGGFRA*FYRA*SSDEVSLAKYRPSPW TSIYY*SLSFISPIVTKRAALYVF*KSQKVQNK GDIKRL

Frame 1

GAGTARRVSPPPRPGPPEGREGTVPYKPRLPAL GRPRRLCCTGGGEGTPGASGKRGPAATTSLVLC IPSVPPPVPFPTLWPPPSWRRQPPGGIRRDFSRR LRREANLVATCLPVRASLPHRLNMLRGPGPGLL LLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPV AVSQSKPGCYDNGKHYQINQQWERTYLGNALVCT CYGGSRGFNCESKPEAEETCFDKYTGNTYRVGD TYERPKDSMIWDCTCIGAGRGRISCTIANRCHE GGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEW TCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMM VDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIG DTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQ TTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVT DSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQ ETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTE GRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQT RGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNM KWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVM YRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIA YSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNC TCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWE KYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSG PVEVFITETPSQPNSHPIQWNAPQPSHISKYIL RWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVY EGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNT VTGETTPFSPLVATSESVTEITASSFVVSWVSA SDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNI PDLLPGRKYIVNVYQISEDGEQSLILSTSQTTA PDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRI VYSPSVEGSSTELNLPETANSVTLSDLQPGVQYN ITIYAVEENQESTPVVIQQETTGTPRSDTVPSP RDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIP VNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKV FAVSHGRESKPLTAQQTTKLDAPTNLQFVNETD STVLVRWTPPRAQITGYRLTVGLTRRGQPRQYN VGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPK ATGVFTTLQPGSSIPPYNTEVTETTIVITWTPA PRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGL TPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPP TNLHLEANPDTGVLTVSWERSTTPDITGYRITT TPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEY NVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVD ITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIP IFEDFVDSSVGYYTVTGLEPGIDYDISVITLIN GGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVT WAPPPSIDLTNFLVRYSPVKNEEDVAELSISPS DNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRG RQKTGLDSPTGIDFSDITANSFTVHWIAPRATIT GYRIRHHPEHFSGRPREDRVPHSRNSITLTNLT PGTEYVVSIVALNGREESPLLIGQQSTVSDVPR DLEVVAATPTSLLISWDAPAVTVRYYRITYGETG GNSPVQEFTVPGSKSTATISGLKPGVDYTITVY AVTGRGDSPASSKPISINYRTEIDKPSQMQVTD VQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTK TKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSG ESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAW ESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDT AELQGLRPGSEYTVSVVALHDDMESQPLIGTQS TAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGY RVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVAT KYEVSVYALKDTLTSRPAQGVVTTLENVSPPRR ARVTDATETTITISWRTKTETITGFQVDAVPAN GQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTL NDNARSSPVVIDASTAIDAPSNLRFLATTPNSL LVSWQPPRARITGYIIKYEKPGSPPREVVPRPR PGVTEATITGLEPGTEYTIYVIALKNNQKSEPLI GRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQP SVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYP PNVGQEALSQTTISWAPFQDTSEYIISCHPVGTD EEPLQFRVPGTSTSATLTGLTRGATYNVIVEAL KDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFD PYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGH FRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMS CTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQW QKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSP EGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPL DVQADREDSRE*IIFPIQRNKHVSLPRSI*TGV MLADPA*SSSFFLKPFALEEVLQLQLNSQLLQAS PWEFPEGFLINEGCTLPVLLRSIQYRSVF*MK* F*DLVWDQ*ESICSQPRCKCFEMI*PKF*VGKS PKHFCFHLSVWPAIL*EQA*SCYCDILNIHSTHF FQMILVIA*KYLSLTCYLSIFPSIFIRKKLY*K HLVCS**EEFGIIMVGDYFLYCMCQSFTTVERQ LF**KIYIPQLEVHLFDIRHLRGK*FL*IFFFNS ANI*KSMMCKSNC*FQYKIF*ISCYKN*LVFVT SSLH*WR*LYTFCFNRFKWTPIFIKIASLPFRS IIEIIFSCSFLTIVILPFFPPPFLH*INLCSKRL PAREIKMTKILKVFE*YNMEEFYLRETPWYDNP HLTCLTLGQLTLLAWLDKLPAKQFVYS*SVKVI SFSI*H*GNKLWD*I*HSGQAQWLTPLIPALWEA EAGGSPEVRSLRPAWPTW*NLVSIKNTNVTRHG SGRLWSRLLRRLR*ENGLNPEGGGCSELRLCH* TPAWGTEQDSIPKNINK*M*HSE**IVEMEIIIS KKLVVHNKQDMQSTF*IY*CTMKEGISINTWQR ILKLNTERYFLMFI*KKKILTPI*IDLQFIKCF DI*LSHLGEVLELNFTGHEAQMRFP*RNTDPAPG LLFITDL*ALSAQ*SPRELLCMFFKNHRKYKIK VILNDC

Frame 2

GRGQPGGSLLPRAPGLQRGGRGPSHISPGSRRS DARAGCAAQGEEREPQARAGRGDLQPQLLWSSA SLLSLHPSPSPPSGPHLLGGDNPREALEGIFPA GCEGKQTWWQLASRCGRLSPTVSTCLGVRGPGCC CWPSSAWGQRCPPREPRRARGRLSKWFSPSPRW LSVKASPVVMTMENTIR*INSGSGPT*AMRWFV LVMEEAEVLTARVNLKLKRLALTSTLGTLTEWVT LMSVLKTP*SGTVPASGLGEGE*AVPSQTAAMK GVSPTRLVTPGGDHMRLVVTC*SVCVLVMEKEN GPASP*LRSVLIMLLGLPMWSEKRGRSPTKAG*W *IVLAWEKAADASLALLEIDATIRTQGHPIELE TPGARRIIEETCSSASAQATAEESGSVRGTPLC RPHRADLAPSPMFVQLFTNRSLTPSLLPMATVSQ TVVWSTLWGCSG*RHKEISKCFARAWATESAAK RQL*PRLTVATQMESHVSYHSPTMAGRSTPAPQ KGDRTDIFGAAQLRIMSRTRNTLSAQTTLFWFRL EEEIPMVPCATSPSYTTTTITLIALLRAEETT* SGVGPHRTMMPTRSLGSAPWLPTRKSAQPMKGS CTALEISGISSMTWVT**GARVLGMVVGNGHALP TRSFEISALLMTSLTM*TTHSTSVMKRGTC*TV HASVRVGAGGSVIPSTNARIQRLGRFIKLEIHG RSMCMVSDTSATAMAVALGSGIANLYRPIQAQVV LSKYLSLRLRVSPTPTPSSGMHHSHLTFPSTFS GGDLKIL*AVGRKLPYQAT*TPTPSKA*SLVWY TRASSSASSSTATKK*LALTSPPPAPAHL*PATP *QERRLPFLLLWPLLNL*PKSQPVALWSPGSQL PTPCRDSGWNMS*VRREMSHSTWIFQAQPLL*T SLTCFLAENTL*MSIRYLRMGSRV*SCLLHKQQR LMPLLTRLWTKLMTPQLLFAGADPRLPSQGTE* SIRHQ*KVAAQNSTFLKLQTPSPSVTCNLVFSI TSLSMLWKKIKKVHLLSFNKKPLAPHAQIQCPLP GTCSLWK*QT*RSPSCGHRLRVQ*PATVWM*SP STCLASTGRGCPSAGTPLQKSPGCPLGSPITSK SLQ*AMGGRASL*LLNRQPNWMLPLTSSLSMKLI LLSW*DGLHLGPR*QDTD*PWALPEEDSPGSTM WVPLSPSTH*GICSLHLSTPYPSWP*RATKRAP KPLESLPHCSLGALFHLTTPR*LRPPL*SHGRLL QELVLSWVYDQAREERHHEK*LQTQEASLCPA* LQE*NTSTPSKS*EMDRKEMRQL*TKW*HHCLH QQTCIWRQTLTLECSQSPGRGAPPQTLLVIELPQ PLQTASREILWKKWSMLIRAPALLIT*VPAWST MSVFTLSRMTRKVSLSLIPSSQRCPNSLT*ALL I*PIQASA*GGPR*TLPPLLGTASQ*LRQEKVSL FLKILWTPQ*DTTQSQGWSRALTMISALSLSLM AARVPLLH*HNKRLFLLPLTCDSPTLVQTPCVS PGLHPHPLI*PTSWCVTHL*KMRKMLQSCQFLLQ TMQWS*QISCLVQNM**VSPVSTNNMRAHLLEE DRKQVLIPQLALTFLILLPTLLLCTGLLLEPPS LATGSAIIPSTSVGDLEKIGCPTLGIPSPSPTSL QAQSMWSASLLLMAERKVPY*LANNQQFLMFRG TWKLLLRPPPAY*SAGMLLLSQ*DITGSLTERQ EEIALSRSSLCLGASLQLPSAALNLELIIPSLCM LSLAVETAPQAASQFPLITEQKLTNHPRCK*PM FRTTALVSSGCLQVPLLLVTE*PPLPKMDQDQQ KLKLQVQIKQK*LLKACSPQWSMWLVSMLRIQAE RVSLWFRLQ*PTLIALKDWHSLMWMSIPSKLLG KAHRGKFPGTG*PTRALRMESMSYSLHLMVKKT LQSCKASDRVLSTQSVWLPCTMIWRASP*LEPSP QLFLHQLT*SSLRSHPQA*APSGHHPMFSSLDI ECG*PPRRRPDQ*KKSTLLLTAHPWLYQDLWWP PNMK*VSMLLRTL*QADQLRELSPLWRMSAHQEG LV*QMLLRPPSPLAGEPRLRRSLASKLMPFQPM ARLQSREPSSQMSEATPSQVYNQALTTRSTCTP *MTMLGAPLWSSTPPLPLMHHPTCVSWPPHPIPC WYHGSRHVPGLPATSSSMRSLGLLPEKWSLGPA LVSQRLLLLAWNREPNIQFMSLP*RIIRRASP* LEGKRQFKRPLSSPTLGMTLEMVFSFLALLVSNP VLGNK*SLRNMVLGGPHRPQRPPP*GIGQDHTR RM*DKKLSLRQPSHGPHSRTLLSTSFHVILLAL MKNPYSSGFLELLPVPL*QASPEVPPTTS*WRH* KTSRGIRFGKRLLPWATLSTKA*TNLRMTRALT PTQFPIMPLEMSGNECLNQALNCCASA*ALEVV ISDVIHLDGAMTMV*TTRLERSGTVREKMAR**A AHVLGTEKENSSVTLMRQRVMMMGRHTT*ENSG RRNISVPFAPAHALEASGAGAVTTAADLGVNPV PKALLASPTTSILRDTIREQTLMLIAQLSASCL* MYRLTEKIPESKSSFQSRGTSMSLCQDPSKLE* C*QTQLRVLLSFLSPLLWRKFSSFSSTHSFSKH HPGSFLRVFS*MRAAHCLFCFEVFNTAQYFK*SD SKIWFGINRKAYAANQDANVLK*YDQNFK*ESH PNTSAFT*VSGPQYCRNKHDLVTVIF*ISTVLT FSK*S**LPRNIFLLPVIYQFFPVFLYGKNCIEN T*YAVDKRNLV*LWWVIIFYTVCAKALLLWKDN CFNKRFTFHNLKFIYLI*DTFGGNNSCEYSFSI QQTFENL*CASLIVDFSTRFSKSVATKTDWFLSL HLFTNGDSFTLSALIDLSGPQYLLKLLVYRSEV **K*SLVALF*PL*FFPSSLHLSFIE*TSVQRD CLQGK*K*LRY*KYLNSIIWRSFILGKPHGMITP I*HVLLWVS*HFWHGLINSQLNSLFTPDL*RSF LFQFNIEETNFGIKFDIQARRSGSHL*SQHFGR LRQVDHLRSGV*DQPGQHGETLSLLKIQMLPGMA VGVFGPGYSEG*GRRMA*TQKAEVAVS*DCAIE LQHGGQSKTPSQKT*INKCDIQNSK*WKWK*LL VKN**FIINKTCKALFKYTNVQ*KKVFQ*TPGKE Y*N*IQKDTSSCSFEKKKY*LPFELIYSL*NAL TFSYLI*GRF*SLILQGMKLR*GFLSEIQTQPL DFYLLLIFKLYQPNSHQESCSVCFLKITESTK*R *Y*TTV