Back to Rnomics Platform
 Open Session
×
1 warning:
  • Access restricted to public data.
    Please sign in at Rnomics Platform to access your data.
Navigation
Home
Sequences

Sequence TUBB3.iAug05

GAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAG
TGGGGCCGGCAACAACTGGGCCAAGGGTCACTACACGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGG
CACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTG
TCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGCGCTCTACG
ACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTT
CCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCC
CGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCC
GCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACTTCGT
GGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAG
GAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCA
CCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGACGAGGA
GGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACCCCCGGAGC
CATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGCCACGTGTG
AGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACTTAATAAAT
CTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

EPFWWTWNPEPWTVSAQGPLDISSGLTISSLVR
VGPATTGPRVTTRKECENCDCLQGFQLTHSLGGG
TGSGMGTLLISKVREEYPDRIMNTFSVVPSPKV
SDTVVEPYNATLSIHQLVENTDETYCIDNEALY
DICFRTLKLATPTYGDLNHLVSATMSGVTTSLRF
PGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTA
RGSQQYRALTVPELTQQMFDAKNMMAACDPRHG
RYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFV
EWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQ
ELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEF
TEAESNMNDLVSEYQQYQDATAEEEGEMYEDDEE
ESEAQGPK*SCSQLE*EAGGGRGRSQQCLNPRS
HLAADTLLSPRPRAPLPPSCSIYGLVLPT*ATC
ELLLSLSYCSSRPDVLRFCFLLVCVYIFGDT**I
YCCQIPL

Frame 1

SHSGGPGTRNHGQCPLRGLWTSLQA*QFHLWSE
WGRQQLGQGSLHGRSVKTATACRASS*PTRWGA
ARAPAWARCSSARCVRSIPTAS*TPSASCPHPRC
QTRWWSPTTPRCPSTSWWRTRMRPTASTTRRST
TSASAPSSWPRPPTGTSTTWYRPP*AESPPPCA
SRASSTLTCASWPSTWCPSRACTSSCPASPPSQP
GAASSTGP*PCPSSPSRCSMPRT*WPPATRATA
AT*RWPPCSGAACP*RRWTSRCWPSRARTAATS
WSGSPTT*RWPCVTSRPAASRCPPPSSGTARPSR
SCSSASPSSSRPCSGARPSCTGTRARAWTRWSS
PRPRAT*TTWCPSTSSTRTPRPRKRARCTKTTR
RSRRPRAPSEAARSWSERQVAAGAEASSV*TPGA
ILLPTPCFPLALGLPCRPPAVFMASSSPPRPRV
SCSCLCLIAAPGLTFYGFVFYWFVFIFSGILNK
SIAVRYP

Frame 2

AILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQS
GAGNNWAKGHYTEGV*KLRLPAGLPADPLAGGR
HGLRHGHVAHQQGA*GVSRPHHEHLQRRALTQG
VRHGGGALQRHAVHPPAGGEHG*DLLHRQRGALR
HLLPHPQAGHAHLRGPQPPGIGHHERSHHLLAL
PGPAQR*PAQAGRQHGALPAPALLHARLRPPHS
PGQPAVPGPDRARAHPADVRCQEHDGRLRPAPRP
LPDGGHRVPGPHVHEGGGRADAGHPEQEQQLLR
GVDPQQREGGRV*HPAPRPQDVLHLHREQHGHP
GAVQAHLRAVHGHVPAQGLPALVHGRGHGRDGVH
RGREQHERPGVRVPAVPGRHGRGRGRDVRRRRG
GVGGPGPQVKLLAAGVRGRWRPGPKPAVSKPPE
PSCCRHPAFPSP*GSLAALLQYLWPRPPHLGHV*
AAPVSVLLQLQA*RFTVLFFTGLCLYFRGYLIN
LLLSDTL

Sequence TUBB3.jAug05

CCTGTCCACTCTGAGATGCCTGGAAGGGGGCTCAGTGCAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGAC
TCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCA
TGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTCACTA
CACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTG
GGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCAC
CCAAGGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGC
GCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCC
TTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCC
TCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCG
CCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGC
TACTTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGG
CCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGAT
GGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGAC
GACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACC
CCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGC
CACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACT
TAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

PVHSEMPGRGLSAVLGSHQ**AWHRPQRQLRGR
LGLAAGADQRLLQRGLFSQVRASSHSGGPGTRNH
GQCPLRGLWTSLQA*QFHLWSEWGRQQLGQGSL
HGGGGAGGFGPGCGAEGV*KLRLPAGLPADPLA
GGRHGLRHGHVAHQQGA*GVSRPHHEHLQRRALT
QGVRHGGGALQRHAVHPPAGGEHG*DLLHRQRG
ALRHLLPHPQAGHAHLRGPQPPGIGHHERSHHL
LALPGPAQR*PAQAGRQHGALPAPALLHARLRPP
HSPGQPAVPGPDRARAHPADVRCQEHDGRLRPA
PRPLPDGGHRVPGPHVHEGGGRADAGHPEQEQQ
LLRGVDPQQREGGRV*HPAPRPQDVLHLHREQHG
HPGAVQAHLRAVHGHVPAQGLPALVHGRGHGRD
GVHRGREQHERPGVRVPAVPGRHGRGRGRDVRR
RRGGVGGPGPQVKLLAAGVRGRWRPGPKPAVSKP
PEPSCCRHPAFPSP*GSLAALLQYLWPRPPHLG
HV*AAPVSVLLQLQA*RFTVLFFTGLCLYFRGY
LINLLLSDTL

Frame 1

LSTLRCLEGGSVQFWEVISDEHGIDPSGNYVGD
SDLQLERISVYYNEASSHKYVPRAILVDLEPGT
MDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHY
TEGAELVDSVLDVVRKECENCDCLQGFQLTHSL
GGGTGSGMGTLLISKVREEYPDRIMNTFSVVPS
PKVSDTVVEPYNATLSIHQLVENTDETYCIDNEA
LYDICFRTLKLATPTYGDLNHLVSATMSGVTTS
LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAP
LTARGSQQYRALTVPELTQQMFDAKNMMAACDPR
HGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSS
YFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNST
AIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEM
EFTEAESNMNDLVSEYQQYQDATAEEEGEMYED
DEEESEAQGPK*SCSQLE*EAGGGRGRSQQCLN
PRSHLAADTLLSPRPRAPLPPSCSIYGLVLPT*A
TCELLLSLSYCSSRPDVLRFCFLLVCVYIFGDT
**IYCCQIPL

Frame 2

CPL*DAWKGAQCSSGKSSVMSMASTPAATTWAT
RTCSWSGSASTTTRPLLTSTCLEPFWWTWNPEP
WTVSAQGPLDISSGLTISSLVRVGPATTGPRVT
TRRGRSWWIRSWMWCGRSVKTATACRASS*PTRW
GAARAPAWARCSSARCVRSIPTAS*TPSASCPH
PRCQTRWWSPTTPRCPSTSWWRTRMRPTASTTR
RSTTSASAPSSWPRPPTGTSTTWYRPP*AESPPP
CASRASSTLTCASWPSTWCPSRACTSSCPASPP
SQPGAASSTGP*PCPSSPSRCSMPRT*WPPATR
ATAAT*RWPPCSGAACP*RRWTSRCWPSRARTAA
TSWSGSPTT*RWPCVTSRPAASRCPPPSSGTAR
PSRSCSSASPSSSRPCSGARPSCTGTRARAWTR
WSSPRPRAT*TTWCPSTSSTRTPRPRKRARCTKT
TRRSRRPRAPSEAARSWSERQVAAGAEASSV*T
PGAILLPTPCFPLALGLPCRPPAVFMASSSPPR
PRVSCSCLCLIAAPGLTFYGFVFYWFVFIFSGIL
NKSIAVRYP

Sequence TUBB3.hAug05

GATGGAGCAGGAGCTGGCTGCCCTGAGGCTGCAAAACTTCTTCCCTCGTGGAGACAGGGAGGCACCTCAGACACTCACCCCGGACTCCCTTGAACAGGGA
CAGGGAGGAACCCCAGGCAGCTAGACCCCAGCAGCAGCCACACGAGCACACTGTGGGGCAGGGAGGGGCATCTCTTGAGAACAAAAGATCCATTTCTCGA
CTTTCCAAACTGGAGAGCTTCTTGAGAGAAAAGAGAGAGACAGGTACAGGTCCACGCCACCCACACACAGCCCTGTGCACACAGACCGGACACAGGCGTC
CACAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTA
CAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTC
TTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGG
ATGTGGTGCGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCT
CATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCCTACAAC
GCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGG
CCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCG
CAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTG
ACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCC
GGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTGAAGGT
GGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAG
TTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACC
TGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTG
AAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACCCCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCC
CCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGCCACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCA
GCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACTTAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

DGAGAGCPEAAKLLPSWRQGGTSDTHPGLP*TG
TGRNPRQLDPSSSHTSTLWGREGHLLRTKDPFLD
FPNWRAS*EKRERQVQVHATHTQPCAHRPDTGV
HSSGKSSVMSMASTPAATTWATRTCSWSGSAST
TTRPLLTSTCLEPFWWTWNPEPWTVSAQGPLDIS
SGLTISSLVRVGPATTGPRVTTRRGRSWWIRSW
MWCGRSVKTATACRASS*PTRWGAARAPAWARC
SSARCVRSIPTAS*TPSASCPHPRCQTRWWSPTT
PRCPSTSWWRTRMRPTASTTRRSTTSASAPSSW
PRPPTGTSTTWYRPP*AESPPPCASRASSTLTC
ASWPSTWCPSRACTSSCPASPPSQPGAASSTGP*
PCPSSPSRCSMPRT*WPPATRATAAT*RWPPCS
GAACP*RRWTSRCWPSRARTAATSWSGSPTT*R
WPCVTSRPAASRCPPPSSGTARPSRSCSSASPSS
SRPCSGARPSCTGTRARAWTRWSSPRPRAT*TT
WCPSTSSTRTPRPRKRARCTKTTRRSRRPRAPS
EAARSWSERQVAAGAEASSV*TPGAILLPTPCFP
LALGLPCRPPAVFMASSSPPRPRVSCSCLCLIA
APGLTFYGFVFYWFVFIFSGILNKSIAVRYP

Frame 1

MEQELAALRLQNFFPRGDREAPQTLTPDSLEQG
QGGTPGS*TPAAATRAHCGAGRGIS*EQKIHFS
TFQTGELLERKERDRYRSTPPTHSPVHTDRTQAS
TVLGSHQ**AWHRPQRQLRGRLGLAAGADQRLL
QRGLFSQVRASSHSGGPGTRNHGQCPLRGLWTS
LQA*QFHLWSEWGRQQLGQGSLHGGGGAGGFGPG
CGAEGV*KLRLPAGLPADPLAGGRHGLRHGHVA
HQQGA*GVSRPHHEHLQRRALTQGVRHGGGALQ
RHAVHPPAGGEHG*DLLHRQRGALRHLLPHPQAG
HAHLRGPQPPGIGHHERSHHLLALPGPAQR*PA
QAGRQHGALPAPALLHARLRPPHSPGQPAVPGP
DRARAHPADVRCQEHDGRLRPAPRPLPDGGHRVP
GPHVHEGGGRADAGHPEQEQQLLRGVDPQQREG
GRV*HPAPRPQDVLHLHREQHGHPGAVQAHLRA
VHGHVPAQGLPALVHGRGHGRDGVHRGREQHERP
GVRVPAVPGRHGRGRGRDVRRRRGGVGGPGPQV
KLLAAGVRGRWRPGPKPAVSKPPEPSCCRHPAF
PSP*GSLAALLQYLWPRPPHLGHV*AAPVSVLLQ
LQA*RFTVLFFTGLCLYFRGYLINLLLSDTL

Frame 2

WSRSWLP*GCKTSSLVETGRHLRHSPRTPLNRD
REEPQAARPQQQPHEHTVGQGGASLENKRSISR
LSKLESFLREKRETGTGPRHPHTALCTQTGHRR
PQFWEVISDEHGIDPSGNYVGDSDLQLERISVYY
NEASSHKYVPRAILVDLEPGTMDSVRSGAFGHL
FRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVL
DVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLL
ISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYN
ATLSIHQLVENTDETYCIDNEALYDICFRTLKL
ATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLR
KLAVNMVPFPRLHFFMPGFAPLTARGSQQYRAL
TVPELTQQMFDAKNMMAACDPRHGRYLTVATVF
RGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKV
AVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQ
FTAMFRRKAFLHWYTGEGMDEMEFTEAESNMND
LVSEYQQYQDATAEEEGEMYEDDEEESEAQGPK*
SCSQLE*EAGGGRGRSQQCLNPRSHLAADTLLS
PRPRAPLPPSCSIYGLVLPT*ATCELLLSLSYC
SSRPDVLRFCFLLVCVYIFGDT**IYCCQIPL

Sequence TUBB3.eAug05

AAGAGCGCGCGGCCGCGGTCCCCGACCCTCAGCAGCCAGCCCGGCCCGCCCGCGCCCGTCCGCAGCCGCCCGCCAGACGCGCCCAGTATGAGGGAGATCG
TGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGG
CGACTCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTAGACAGTCTCGATATGTTGCCCAGGCTGGTCTCGAACTCCTGGACTC
AAGCGATCCACCTGCCTTGGCCTCCCAAAGTGTTGGGAGTGCAGACACGAGCCCCTGCATCTGGTGGACGTCTGACTGAATCCTGCCTGTCCTGCTCCGT
CCTGTCCTGCTCCGTCCTTAAACACAGTGAGGCTTAAGGGTGAAGAAAGTTGCTGAAGCTTGCCTTTGGTCCAGGACTCTGACTTCAGAGTACAGGCTCT
TAGGATCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCC
TGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTG
CGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCA
AGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCT
GTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCC
ACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGG
CCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCC
CGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGC
ATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGT
GTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGC
CATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCC
GAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCT
CGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACCCCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCC
TAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGCCACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGG
CCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACTTAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

KSARPRSPTLSSQPGPPAPVRSRPPDAPSMREI
VHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVG
DSDLQLERISVYYNEAS*TVSICCPGWSRTPGL
KRSTCLGLPKCWECRHEPLHLVDV*LNPACPAP
SCPAPSLNTVRLKGEESC*SLPLVQDSDFRVQAL
RISQVRASSHSGGPGTRNHGQCPLRGLWTSLQA
*QFHLWSEWGRQQLGQGSLHGGGGAGGFGPGCG
AEGV*KLRLPAGLPADPLAGGRHGLRHGHVAHQQ
GA*GVSRPHHEHLQRRALTQGVRHGGGALQRHA
VHPPAGGEHG*DLLHRQRGALRHLLPHPQAGHA
HLRGPQPPGIGHHERSHHLLALPGPAQR*PAQAG
RQHGALPAPALLHARLRPPHSPGQPAVPGPDRA
RAHPADVRCQEHDGRLRPAPRPLPDGGHRVPGP
HVHEGGGRADAGHPEQEQQLLRGVDPQQREGGRV
*HPAPRPQDVLHLHREQHGHPGAVQAHLRAVHG
HVPAQGLPALVHGRGHGRDGVHRGREQHERPGV
RVPAVPGRHGRGRGRDVRRRRGGVGGPGPQVKLL
AAGVRGRWRPGPKPAVSKPPEPSCCRHPAFPSP
*GSLAALLQYLWPRPPHLGHV*AAPVSVLLQLQ
A*RFTVLFFTGLCLYFRGYLINLLLSDTL

Frame 1

RARGRGPRPSAASPARPRPSAAARQTRPV*GRS
CTSRPASAATRSGPSSGKSSVMSMASTPAATTW
ATRTCSWSGSASTTTRPLRQSRYVAQAGLELLDS
SDPPALASQSVGSADTSPCIWWTSD*ILPVLLR
PVLLRP*TQ*GLRVKKVAEACLWSRTLTSEYRL
LGSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRP
DNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKECENCDCLQGFQLTHSLGGGTGSGMGTLLIS
KVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATL
SIHQLVENTDETYCIDNEALYDICFRTLKLATP
TYGDLNHLVSATMSGVTTSLRFPGQLNADLRKL
AVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVP
ELTQQMFDAKNMMAACDPRHGRYLTVATVFRGR
MSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAV
CDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTA
MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATAEEEGEMYEDDEEESEAQGPK*SC
SQLE*EAGGGRGRSQQCLNPRSHLAADTLLSPRP
RAPLPPSCSIYGLVLPT*ATCELLLSLSYCSSR
PDVLRFCFLLVCVYIFGDT**IYCCQIPL

Frame 2

ERAAAVPDPQQPARPARARPQPPARRAQYEGDR
AHPGRPVRQPDRGQVLGSHQ**AWHRPQRQLRG
RLGLAAGADQRLLQRGLLDSLDMLPRLVSNSWT
QAIHLPWPPKVLGVQTRAPASGGRLTESCLSCSV
LSCSVLKHSEA*G*RKLLKLAFGPGL*LQSTGS
*DLTSTCLEPFWWTWNPEPWTVSAQGPLDISSG
LTISSLVRVGPATTGPRVTTRRGRSWWIRSWMWC
GRSVKTATACRASS*PTRWGAARAPAWARCSSA
RCVRSIPTAS*TPSASCPHPRCQTRWWSPTTPR
CPSTSWWRTRMRPTASTTRRSTTSASAPSSWPRP
PTGTSTTWYRPP*AESPPPCASRASSTLTCASW
PSTWCPSRACTSSCPASPPSQPGAASSTGP*PC
PSSPSRCSMPRT*WPPATRATAAT*RWPPCSGAA
CP*RRWTSRCWPSRARTAATSWSGSPTT*RWPC
VTSRPAASRCPPPSSGTARPSRSCSSASPSSSR
PCSGARPSCTGTRARAWTRWSSPRPRAT*TTWCP
STSSTRTPRPRKRARCTKTTRRSRRPRAPSEAA
RSWSERQVAAGAEASSV*TPGAILLPTPCFPLA
LGLPCRPPAVFMASSSPPRPRVSCSCLCLIAAPG
LTFYGFVFYWFVFIFSGILNKSIAVRYP

Sequence TUBB3.dAug05

ATTCTATGAGACAGAGTCTCACTCTGTCGCCCACGCTGGAGTACAGTGGTGCAATCTTGGCTTACTGCAACCTCCGTCTCCTGGGTTCAAGCAATTCTTC
TGCCTCAGCTTCCGGAGTAGCTGGAACGTGTGAGCCACCACCCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGCTGGTCTCGAACTCCTGACTTCCG
GTGATCTGCCCACCTCAGCCTCCCAAATTGTTGGTATTACAGGCGTGAGCCACCGCACCTGGCCTCTATTTATTTATTTATTTTGAGATGGGGTCTTGAT
ATGAATTCCTAGACTCATGCTTCTCATGCTTGCTACCACCCAGTCCTGCCTCCTGCACAGCAGTTTCCACTAAAGTCACCCCTTTACGAGAGGCATCTTT
TTTTTTTTTTTGAGATGGTGTCTCACTCTGTCGCCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCAAGCTCCACCTCCTGGGTTCAAGTGATT
CTCCTGCTTCAGCCTCCCGAGTAGCTGGGACTACAGGCACCCGCCACCATTCCCGGCTAATTTTTTGTATCTTTAGTAGAGACGGGGTTTCACCATGTTA
GCCAGGATGGTCTCGATCTCCTGACTTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGTACCTGGCCTACTAGAG
GCATCTTAAAACACATCTCCTGTGTCTCCATTCCAAAGCGCATCTTTGTCTACACTCCTGGGGTCCTCCCACGTCAGCCTCCCAAAGTGCTGGGATTACA
GTCATGAGCCACTGTGCCCGGCCCCACCAAATTGTTTTCTAACCTGCTTTTTTCTTGAGGAATCTTCTGCAGCACATGACTCACATGTAGAGCCCCTTGT
TTCTTGAGGCCTTCATACCCGTCAGCTCATCTAAAAGTCACTCCTGAGGAGTAAGGGCAAGGGGCATTGGGAGAGGTCAGGCAGCCCTGGGAAGCCTCCC
AGCGTGTGTCCCAGGAGGCCTGCTGCCTGCAGACACTGCTCCCAGACGCAGCGGCCTCGGCCATCAGAGCGTGTCCCACGATGAGGTGGCAACTTCCCTG
AAGGGCTTGCTCTCACCCAGGGGTGTGGTTCTCCGTGTGCACACAGATAGATAGTTCTACGTTTGCCATATACCATTTATATGCCTCACACAGATTAACC
CACTTTCATTTCAAAAAGACCTCCTGTCATCGTGCCCATTGTACAGATGAGGACACTGAGACACGGAATCCTAGTGACCTGTGCAAGGTCACACAGCTGG
GAAGGGTGAGGGAAGCGTCTGTGAACCCAGCAGGCCCAGCCCCTGAGTCTAGGCTCCGCTCGGCAGCTGTCATGGGAGCAGAGTCCGAGAAGCACTGCAG
GCACCTGCTCTAGGGGGAGAGAGGTGGAGGTGGCAGGTAGAGAGAGGACGACCCCTGGGAGCTGAGACGATGCCAGCCTCACACGGACAGGCTGGGTGAC
CTCAGGCAAGTCGCTTCCCATTTGGGACCCCAAGGACCCTTCTGCAGACCCAGCTGGGGGTCCCCGATGAGACTGGCTCTCAGAGTCCCTGTGAGTCCCG
TCAGCTGAGGCCAGTAATGTCCTGGCACCATCACTGGACATGGTTTGCTCTCCCATGGAGGGGCAGGTGTAGCAGCCAAAAGCGATCCTGAGCTCTGGGC
CTCTGAACCCTGCTCTGCCAGCTACTGTGGGTGTCTCTACCCGCTCTGTGTCTCAGTTCCCTTTCCTGAAAAGAGGGGATAGTAAAAACTAACCCTCAAG
GTGTTTATTACCAATGCCCTTCATGGAGGAATCCGCTGCTCCAGGCCCTTGCCAGCAGCTGCACAGCTGGGCCTGGGGACCTGGGTGTGGGTCTGTTGAA
CCTTGGCCCACCCTGGGGCTGTGGTCGGCCTGGACGTGTAGGTGTGGAGGTGCATATTTATTATGGCAATTTTCTGACAGGTAACAGAGTGTGGGGCTCT
CTTCTGAATGGGGCTCGTGGAGGCCGTGGGTCAAAAGCCCTAATTTTGTAGTGAGGGCTAAAAGGCTTCACAAGGGAAAGGGCCTGGCTGGGGCTATGGG
CCGGTGCCGACCCCCCCTCTCCCACTTTGTTTGCAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCGG
ACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGA
CAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTCACTACACG
GAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGG
GCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAA
GGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGCGCTC
TACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGC
GCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCAC
AGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCGCCAC
GGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACT
TCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCAT
CCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAG
TTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGACG
AGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACCCCCG
GAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGCCACG
TGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACTTAAT
AAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

IL*DRVSLCRPRWSTVVQSWLTATSVSWVQAIL
LPQLPE*LERVSHHPWLIFVFLVETGWSRTPDFR
*SAHLSLPNCWYYRREPPHLASIYLFILRWGLD
MNS*THASHACYHPVLPPAQQFPLKSPLYERHL
FFFFEMVSHSVAQAGVQWHDLSSLQAPPPGFK*F
SCFSLPSSWDYRHPPPFPANFLYL**RRGFTML
ARMVSIS*LRDPPASASQSVGITGMSHCTWPTR
GILKHISCVSIPKRIFVYTPGVLPRQPPKVLGLQ
S*ATVPGPTKLFSNLLFS*GIFCST*LTCRAPC
FLRPSYPSAHLKVTPEE*GQGALGEVRQPWEAS
QRVSQEACCLQTLLPDAAASAIRACPTMRWQLP*
RACSHPGVWFSVCTQIDSSTFAIYHLYASHRLT
HFHFKKTSCHRAHCTDEDTETRNPSDLCKVTQL
GRVREASVNPAGPAPESRLRSAAVMGAESEKHCR
HLL*GERGGGGR*REDDPWELRRCQPHTDRLGD
LRQVASHLGPQGPFCRPSWGSPMRLALRVPVSP
VS*GQ*CPGTITGHGLLSHGGAGVAAKSDPELWA
SEPCSASYCGCLYPLCVSVPFPEKRG**KLTLK
VFITNALHGGIRCSRPLPAAAQLGLGTWVWVC*
TLAHPGAVVGLDV*VWRCIFIMAIF*QVTECGAL
F*MGLVEAVGQKP*FCSEG*KASQGKGPGWGYG
PVPTPPLPLCLQFWEVISDEHGIDPSGNYVGDS
DLQLERISVYYNEASSHKYVPRAILVDLEPGTMD
SVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYT
EGAELVDSVLDVVRKECENCDCLQGFQLTHSLG
GGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPK
VSDTVVEPYNATLSIHQLVENTDETYCIDNEAL
YDICFRTLKLATPTYGDLNHLVSATMSGVTTSL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT
ARGSQQYRALTVPELTQQMFDAKNMMAACDPRH
GRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSY
FVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAI
QELFKRISEQFTAMFRRKAFLHWYTGEGMDEME
FTEAESNMNDLVSEYQQYQDATAEEEGEMYEDD
EEESEAQGPK*SCSQLE*EAGGGRGRSQQCLNPR
SHLAADTLLSPRPRAPLPPSCSIYGLVLPT*AT
CELLLSLSYCSSRPDVLRFCFLLVCVYIFGDT*
*IYCCQIPL

Frame 1

FYETESHSVAHAGVQWCNLGLLQPPSPGFKQFF
CLSFRSSWNV*ATTPG*FLYF**RQAGLELLTS
GDLPTSASQIVGITGVSHRTWPLFIYLF*DGVLI
*IPRLMLLMLATTQSCLLHSSFH*SHPFTRGIF
FFFLRWCLTLSPRLECNGMISAHCKLHLLGSSD
SPASASRVAGTTGTRHHSRLIFCIFSRDGVSPC*
PGWSRSPDFVIHPPRPPKVLGLQA*ATVPGLLE
AS*NTSPVSPFQSASLSTLLGSSHVSLPKCWDY
SHEPLCPAPPNCFLTCFFLEESSAAHDSHVEPLV
S*GLHTRQLI*KSLLRSKGKGHWERSGSPGKPP
SVCPRRPAACRHCSQTQRPRPSERVPR*GGNFP
EGLALTQGCGSPCAHR*IVLRLPYTIYMPHTD*P
TFISKRPPVIVPIVQMRTLRHGILVTCARSHSW
EG*GKRL*TQQAQPLSLGSARQLSWEQSPRSTA
GTCSRGREVEVAGRERTTPGS*DDASLTRTGWVT
SGKSLPIWDPKDPSADPAGGPR*DWLSESL*VP
SAEASNVLAPSLDMVCSPMEGQV*QPKAILSSG
PLNPALPATVGVSTRSVSQFPFLKRGDSKN*PSR
CLLPMPFMEESAAPGPCQQLHSWAWGPGCGSVE
PWPTLGLWSAWTCRCGGAYLLWQFSDR*QSVGL
SSEWGSWRPWVKSPNFVVRAKRLHKGKGLAGAMG
RCRPPLSHFVCSSGKSSVMSMASTPAATTWATR
TCSWSGSASTTTRPLLTSTCLEPFWWTWNPEPW
TVSAQGPLDISSGLTISSLVRVGPATTGPRVTTR
RGRSWWIRSWMWCGRSVKTATACRASS*PTRWG
AARAPAWARCSSARCVRSIPTAS*TPSASCPHP
RCQTRWWSPTTPRCPSTSWWRTRMRPTASTTRRS
TTSASAPSSWPRPPTGTSTTWYRPP*AESPPPC
ASRASSTLTCASWPSTWCPSRACTSSCPASPPS
QPGAASSTGP*PCPSSPSRCSMPRT*WPPATRAT
AAT*RWPPCSGAACP*RRWTSRCWPSRARTAAT
SWSGSPTT*RWPCVTSRPAASRCPPPSSGTARP
SRSCSSASPSSSRPCSGARPSCTGTRARAWTRWS
SPRPRAT*TTWCPSTSSTRTPRPRKRARCTKTT
RRSRRPRAPSEAARSWSERQVAAGAEASSV*TP
GAILLPTPCFPLALGLPCRPPAVFMASSSPPRPR
VSCSCLCLIAAPGLTFYGFVFYWFVFIFSGILN
KSIAVRYP

Frame 2

SMRQSLTLSPTLEYSGAILAYCNLRLLGSSNSS
ASASGVAGTCEPPPLANFCIFSRDRLVSNS*LP
VICPPQPPKLLVLQA*ATAPGLYLFIYFEMGS*
YEFLDSCFSCLLPPSPASCTAVSTKVTPLREASF
FFF*DGVSLCRPGWSAMA*SQLTASSTSWVQVI
LLLQPPE*LGLQAPATIPG*FFVSLVETGFHHV
SQDGLDLLTS*STRLGLPKCWDYRHEPLYLAY*R
HLKTHLLCLHSKAHLCLHSWGPPTSASQSAGIT
VMSHCARPHQIVF*PAFFLRNLLQHMTHM*SPL
FLEAFIPVSSSKSHS*GVRARGIGRGQAALGSLP
ACVPGGLLPADTAPRRSGLGHQSVSHDEVATSL
KGLLSPRGVVLRVHTDR*FYVCHIPFICLTQIN
PLSFQKDLLSSCPLYR*GH*DTES**PVQGHTAG
KGEGSVCEPSRPSP*V*APLGSCHGSRVREALQ
APALGGERWRWQVERGRPLGAETMPASHGQAG*
PQASRFPFGTPRTLLQTQLGVPDETGSQSPCESR
QLRPVMSWHHHWTWFALPWRGRCSSQKRS*ALG
L*TLLCQLLWVSLPALCLSSLS*KEGIVKTNPQ
GVYYQCPSWRNPLLQALASSCTAGPGDLGVGLLN
LGPPWGCGRPGRVGVEVHIYYGNFLTGNRVWGS
LLNGARGGRGSKALIL**GLKGFTRERAWLGLW
AGADPPSPTLFAVLGSHQ**AWHRPQRQLRGRLG
LAAGADQRLLQRGLFSQVRASSHSGGPGTRNHG
QCPLRGLWTSLQA*QFHLWSEWGRQQLGQGSLH
GGGGAGGFGPGCGAEGV*KLRLPAGLPADPLAGG
RHGLRHGHVAHQQGA*GVSRPHHEHLQRRALTQ
GVRHGGGALQRHAVHPPAGGEHG*DLLHRQRGA
LRHLLPHPQAGHAHLRGPQPPGIGHHERSHHLLA
LPGPAQR*PAQAGRQHGALPAPALLHARLRPPH
SPGQPAVPGPDRARAHPADVRCQEHDGRLRPAP
RPLPDGGHRVPGPHVHEGGGRADAGHPEQEQQLL
RGVDPQQREGGRV*HPAPRPQDVLHLHREQHGH
PGAVQAHLRAVHGHVPAQGLPALVHGRGHGRDG
VHRGREQHERPGVRVPAVPGRHGRGRGRDVRRRR
GGVGGPGPQVKLLAAGVRGRWRPGPKPAVSKPP
EPSCCRHPAFPSP*GSLAALLQYLWPRPPHLGH
V*AAPVSVLLQLQA*RFTVLFFTGLCLYFRGYLI
NLLLSDTL

Sequence TUBB3.bAug05

AAGAGCGCGCGGCCGCGGTCCCCGACCCTCAGCAGCCAGCCCGGCCCGCCCGCGCCCGTCCGCAGCCGCCCGCCAGACGCGCCCAGTATGAGGGAGATCG
TGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGG
CGACTCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGA
ACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTC
ACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTC
GCTGGGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCC
TCACCCAAGGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACG
AGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCAC
CTCCTTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCC
CCCCTCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACC
CGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAG
CAGCTACTTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGC
ACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACG
AGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGA
AGACGACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTA
AACCCCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCT
AGGCCACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGA
TACTTAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

KSARPRSPTLSSQPGPPAPVRSRPPDAPSMREI
VHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVG
DSDLQLERISVYYNEASSHKYVPRAILVDLEPG
TMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKG
HYTEGAELVDSVLDVVRKECENCDCLQGFQLTHS
LGGGTGSGMGTLLISKVREEYPDRIMNTFSVVP
SPKVSDTVVEPYNATLSIHQLVENTDETYCIDN
EALYDICFRTLKLATPTYGDLNHLVSATMSGVTT
SLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFA
PLTARGSQQYRALTVPELTQQMFDAKNMMAACD
PRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNS
SYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNS
TAIQELFKRISEQFTAMFRRKAFLHWYTGEGMD
EMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYE
DDEEESEAQGPK*SCSQLE*EAGGGRGRSQQCL
NPRSHLAADTLLSPRPRAPLPPSCSIYGLVLPT
*ATCELLLSLSYCSSRPDVLRFCFLLVCVYIFGD
T**IYCCQIPL

Frame 1

RARGRGPRPSAASPARPRPSAAARQTRPV*GRS
CTSRPASAATRSGPSSGKSSVMSMASTPAATTW
ATRTCSWSGSASTTTRPLLTSTCLEPFWWTWNPE
PWTVSAQGPLDISSGLTISSLVRVGPATTGPRV
TTRRGRSWWIRSWMWCGRSVKTATACRASS*PT
RWGAARAPAWARCSSARCVRSIPTAS*TPSASCP
HPRCQTRWWSPTTPRCPSTSWWRTRMRPTASTT
RRSTTSASAPSSWPRPPTGTSTTWYRPP*AESP
PPCASRASSTLTCASWPSTWCPSRACTSSCPASP
PSQPGAASSTGP*PCPSSPSRCSMPRT*WPPAT
RATAAT*RWPPCSGAACP*RRWTSRCWPSRART
AATSWSGSPTT*RWPCVTSRPAASRCPPPSSGTA
RPSRSCSSASPSSSRPCSGARPSCTGTRARAWT
RWSSPRPRAT*TTWCPSTSSTRTPRPRKRARCT
KTTRRSRRPRAPSEAARSWSERQVAAGAEASSV*
TPGAILLPTPCFPLALGLPCRPPAVFMASSSPP
RPRVSCSCLCLIAAPGLTFYGFVFYWFVFIFSG
ILNKSIAVRYP

Frame 2

ERAAAVPDPQQPARPARARPQPPARRAQYEGDR
AHPGRPVRQPDRGQVLGSHQ**AWHRPQRQLRG
RLGLAAGADQRLLQRGLFSQVRASSHSGGPGTR
NHGQCPLRGLWTSLQA*QFHLWSEWGRQQLGQGS
LHGGGGAGGFGPGCGAEGV*KLRLPAGLPADPL
AGGRHGLRHGHVAHQQGA*GVSRPHHEHLQRRA
LTQGVRHGGGALQRHAVHPPAGGEHG*DLLHRQR
GALRHLLPHPQAGHAHLRGPQPPGIGHHERSHH
LLALPGPAQR*PAQAGRQHGALPAPALLHARLR
PPHSPGQPAVPGPDRARAHPADVRCQEHDGRLRP
APRPLPDGGHRVPGPHVHEGGGRADAGHPEQEQ
QLLRGVDPQQREGGRV*HPAPRPQDVLHLHREQ
HGHPGAVQAHLRAVHGHVPAQGLPALVHGRGHGR
DGVHRGREQHERPGVRVPAVPGRHGRGRGRDVR
RRRGGVGGPGPQVKLLAAGVRGRWRPGPKPAVS
KPPEPSCCRHPAFPSP*GSLAALLQYLWPRPPHL
GHV*AAPVSVLLQLQA*RFTVLFFTGLCLYFRG
YLINLLLSDTL

Sequence TUBB3.aAug05

CCCTGGTCCAGCCCCCAAATCTGCCGAGAGGGCAGGTCCCGGGGAAGCTCCGGACTCCTAGAGGGGCGGCCAGGTGGGGGCCCTGGTGACCAGGACAGAC
TGTGGTGTTTTTTAACGTAAAGGAGATCCGCGGTGTGAGGGACCCCCTGGGTCCTGCACGCCGCCTGGTGGCAGGCCGGGCCATGGTGGGTGCTCACGCC
CCCGGCATGTGGCCGCCCTCAGTGGGAGGGGCTCTGAGAACGACTTTTTAAAACGCAGAGAAAAGCTCCATTCTTCCCAGGACCTCAGCGCAGCCCTGGC
CCAGGAAGGCAGGAGACAGAGGCCAGGACGGTCCAGAGGTGTCGAAATGTCCTGGGGACCTGAGCAGCAGCCACCAGGGAAGAGGCAGGGAGGGAGCTGA
GGACCAGGCTTGGTTGTGAGAATCCCTGAGCCCAGGCGGTAGATGCCAGGAGGTGTCTGGACTGGCTGGGCCATGCCTGGGCTGACCTGTCCAGCCAGGG
AGAGGGTGTGAGGGCAGATCTGGGGGTGCCCAGATGGAAGGAGGCAGGCATGGGGGACACCCAAGGCCCCCTGGCAGCACCATGAACTAAGCAGGACACC
TGGAGGGGAAGAACTGTGGGGACCTGGAGGCCTCCAACGACTCCTTCCTGCTTCCTGGACAGGACTATGGCTGTGCAGGGATCCCAGAGAAGACTTCTGG
GCTCCCTCAACTCCACCCCCACAGCCATCCCCCAGCTGGGGCTGGCTGCCAACCAGACAGGAGCCCGGTGCCTGGAGGTGTCCATCTCTGACGGGCTCTT
CCTCAGCCTGGGGCTGGTGAGCTTGGTGGAGAACGCGCTGGTGGTGGCCACCATCGCCAAGAACCGGAACCTGCACTCACCCATGTACTGCTTCATCTGC
TGCCTGGCCTTGTCGGACCTGCTGGTGAGCGGGAGCAACGTGCTGGAGACGGCCGTCATCCTCCTGCTGGAGGCCGGTGCACTGGTGGCCCGGGCTGCGG
TGCTGCAGCAGCTGGACAATGTCATTGACGTGATCACCTGCAGCTCCATGCTGTCCAGCCTCTGCTTCCTGGGCGCCATCGCCGTGGACCGCTACATCTC
CATCTTCTACGCACTGCGCTACCACAGCATCGTGACCCTGCCGCGGGCGCGGCGAGCCGTTGCGGCCATCTGGGTGGCCAGTGTCGTCTTCAGCACGCTC
TTCATCGCCTACTACGACCACGTGGCCGTCCTGCTGTGCCTCGTGGTCTTCTTCCTGGCTATGCTGGTGCTCATGGCCGTGCTGTACGTCCACATGCTGG
CCCGGGCCTGCCAGCACGCCCAGGGCATCGCCCGGCTCCACAAGAGGCAGCGCCCGGTCCACCAGGGCTTTGGCCTTAAAGGCGCTGTCACCCTCACCAT
CCTGCTGGGCATTTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCATCTCACACTCATCGTCCTCTGCCCCGAGCACCCCACGTGCGGCTGCATCTTCAAG
AACTTCAACCTCTTTCTCGCCCTCATCATCTGCAATGCCATCATCGACCCCCTCATCTACGCCTTCCACAGCCAGGAGCTCCGCAGGACGCTCAAGGAGG
TGCTGACATGCTCCTGCTCTCAGGACCGTGCCCTCGTCAGCTGGGATGTGAAGTCTCTGGGTGGAAGTGTGTGCCAAGAGCTACTCCCACAGCAGCCCCA
GGAGAAGGGGCTTTGTGACCAGAAAGCTTCATCCACAGCCTTGCAGCGGCTCCTGCAAAAGGAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGAC
CCCAGCGGCAACTACGTGGGCGACTCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTC
TGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGG
CAACAACTGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAGGAGTGTGAAAACTGCGACTGCCTGCAG
GGCTTCCAGCTGACCCACTCGCTGGGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGCATCATGA
ACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGA
GACCTACTGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACGGGGACCTCAACCACCTGGTATCGGCC
ACCATGAGCGGAGTCACCACCTCCTTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTCCCGCGCCTGCACT
TCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAA
CATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTG
GCCATCCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGCCTCAAGATGTCCT
CCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTA
CACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACGGCCGAG
GAAGAGGGCGAGATGTACGAAGACGACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCTGGAGTGAGAGGCAGGTGGCGGCCGGG
GCCGAAGCCAGCAGTGTCTAAACCCCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCTCCCTTGCCGCCCTCCTGCAGTATTTA
TGGCCTCGTCCTCCCCACCTAGGCCACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACGTTTTACGGTTTTGTTTTTTACTGGTT
TGTGTTTATATTTTCGGGGATACTTAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

PWSSPQICREGRSRGSSGLLEGRPGGGPGDQDR
LWCFLT*RRSAV*GTPWVLHAAWWQAGPWWVLTP
PACGRPQWEGL*ERLFKTQRKAPFFPGPQRSPG
PGRQETEARTVQRCRNVLGT*AAATREEAGREL
RTRLGCENP*AQAVDARRCLDWLGHAWADLSSQG
EGVRADLGVPRWKEAGMGDTQGPLAAP*TKQDT
WRGRTVGTWRPPTTPSCFLDRTMAVQGSQRRLL
GSLNSTPTAIPQLGLAANQTGARCLEVSISDGLF
LSLGLVSLVENALVVATIAKNRNLHSPMYCFIC
CLALSDLLVSGSNVLETAVILLLEAGALVARAA
VLQQLDNVIDVITCSSMLSSLCFLGAIAVDRYIS
IFYALRYHSIVTLPRARRAVAAIWVASVVFSTL
FIAYYDHVAVLLCLVVFFLAMLVLMAVLYVHML
ARACQHAQGIARLHKRQRPVHQGFGLKGAVTLTI
LLGIFFLCWGPFFLHLTLIVLCPEHPTCGCIFK
NFNLFLALIICNAIIDPLIYAFHSQELRRTLKE
VLTCSCSQDRALVSWDVKSLGGSVCQELLPQQPQ
EKGLCDQKASSTALQRLLQKEFWEVISDEHGID
PSGNYVGDSDLQLERISVYYNEASSHKYVPRAI
LVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAG
NNWAKGHYTEGAELVDSVLDVVRKECENCDCLQ
GFQLTHSLGGGTGSGMGTLLISKVREEYPDRIM
NTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDE
TYCIDNEALYDICFRTLKLATPTYGDLNHLVSA
TMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLH
FFMPGFAPLTARGSQQYRALTVPELTQQMFDAKN
MMAACDPRHGRYLTVATVFRGRMSMKEVDEQML
AIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMS
STFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY
TGEGMDEMEFTEAESNMNDLVSEYQQYQDATAE
EEGEMYEDDEEESEAQGPK*SCSQLE*EAGGGR
GRSQQCLNPRSHLAADTLLSPRPRAPLPPSCSIY
GLVLPT*ATCELLLSLSYCSSRPDVLRFCFLLV
CVYIFGDT**IYCCQIPL

Frame 1

PGPAPKSAERAGPGEAPDS*RGGQVGALVTRTD
CGVF*RKGDPRCEGPPGSCTPPGGRPGHGGCSR
PRHVAALSGRGSENDFLKRREKLHSSQDLSAALA
QEGRRQRPGRSRGVEMSWGPEQQPPGKRQGGS*
GPGLVVRIPEPRR*MPGGVWTGWAMPGLTCPAR
ERV*GQIWGCPDGRRQAWGTPKAPWQHHELSRTP
GGEELWGPGGLQRLLPASWTGLWLCRDPREDFW
APSTPPPQPSPSWGWLPTRQEPGAWRCPSLTGS
SSAWGW*AWWRTRWWWPPSPRTGTCTHPCTASSA
AWPCRTCW*AGATCWRRPSSSCWRPVHWWPGLR
CCSSWTMSLT*SPAAPCCPASASWAPSPWTATS
PSSTHCATTAS*PCRGRGEPLRPSGWPVSSSARS
SSPTTTTWPSCCASWSSSWLCWCSWPCCTSTCW
PGPASTPRASPGSTRGSARSTRALALKALSPSP
SCWAFSSSAGAPSSCISHSSSSAPSTPRAAASSR
TSTSFSPSSSAMPSSTPSSTPSTARSSAGRSRR
C*HAPALRTVPSSAGM*SLWVEVCAKSYSHSSP
RRRGFVTRKLHPQPCSGSCKRSSGKSSVMSMAST
PAATTWATRTCSWSGSASTTTRPLLTSTCLEPF
WWTWNPEPWTVSAQGPLDISSGLTISSLVRVGP
ATTGPRVTTRRGRSWWIRSWMWCGRSVKTATACR
ASS*PTRWGAARAPAWARCSSARCVRSIPTAS*
TPSASCPHPRCQTRWWSPTTPRCPSTSWWRTRM
RPTASTTRRSTTSASAPSSWPRPPTGTSTTWYRP
P*AESPPPCASRASSTLTCASWPSTWCPSRACT
SSCPASPPSQPGAASSTGP*PCPSSPSRCSMPR
T*WPPATRATAAT*RWPPCSGAACP*RRWTSRCW
PSRARTAATSWSGSPTT*RWPCVTSRPAASRCP
PPSSGTARPSRSCSSASPSSSRPCSGARPSCTG
TRARAWTRWSSPRPRAT*TTWCPSTSSTRTPRPR
KRARCTKTTRRSRRPRAPSEAARSWSERQVAAG
AEASSV*TPGAILLPTPCFPLALGLPCRPPAVF
MASSSPPRPRVSCSCLCLIAAPGLTFYGFVFYWF
VFIFSGILNKSIAVRYP

Frame 2

LVQPPNLPRGQVPGKLRTPRGAARWGPW*PGQT
VVFFNVKEIRGVRDPLGPARRLVAGRAMVGAHA
PGMWPPSVGGALRTTF*NAEKSSILPRTSAQPW
PRKAGDRGQDGPEVSKCPGDLSSSHQGRGREGAE
DQAWL*ESLSPGGRCQEVSGLAGPCLG*PVQPG
RGCEGRSGGAQMEGGRHGGHPRPPGSTMN*AGH
LEGKNCGDLEASNDSFLLPGQDYGCAGIPEKTSG
LPQLHPHSHPPAGAGCQPDRSPVPGGVHL*RAL
PQPGAGELGGERAGGGHHRQEPEPALTHVLLHL
LPGLVGPAGEREQRAGDGRHPPAGGRCTGGPGCG
AAAAGQCH*RDHLQLHAVQPLLPGRHRRGPLHL
HLLRTALPQHRDPAAGAASRCGHLGGQCRLQHA
LHRLLRPRGRPAVPRGLLPGYAGAHGRAVRPHAG
PGLPARPGHRPAPQEAAPGPPGLWP*RRCHPHH
PAGHFLPLLGPLLPASHTHRPLPRAPHVRLHLQ
ELQPLSRPHHLQCHHRPPHLRLPQPGAPQDAQGG
ADMLLLSGPCPRQLGCEVSGWKCVPRATPTAAP
GEGAL*PESFIHSLAAAPAKGVLGSHQ**AWHR
PQRQLRGRLGLAAGADQRLLQRGLFSQVRASSHS
GGPGTRNHGQCPLRGLWTSLQA*QFHLWSEWGR
QQLGQGSLHGGGGAGGFGPGCGAEGV*KLRLPA
GLPADPLAGGRHGLRHGHVAHQQGA*GVSRPHHE
HLQRRALTQGVRHGGGALQRHAVHPPAGGEHG*
DLLHRQRGALRHLLPHPQAGHAHLRGPQPPGIG
HHERSHHLLALPGPAQR*PAQAGRQHGALPAPAL
LHARLRPPHSPGQPAVPGPDRARAHPADVRCQE
HDGRLRPAPRPLPDGGHRVPGPHVHEGGGRADA
GHPEQEQQLLRGVDPQQREGGRV*HPAPRPQDVL
HLHREQHGHPGAVQAHLRAVHGHVPAQGLPALV
HGRGHGRDGVHRGREQHERPGVRVPAVPGRHGR
GRGRDVRRRRGGVGGPGPQVKLLAAGVRGRWRPG
PKPAVSKPPEPSCCRHPAFPSP*GSLAALLQYL
WPRPPHLGHV*AAPVSVLLQLQA*RFTVLFFTG
LCLYFRGYLINLLLSDTL

Sequence TUBB3.kAug05

AAGAGCGCGCGGCCGCGGTCCCCGACCCTCAGCAGCCAGCCCGGCCCGCCCGCGCCCGTCCGCAGCCGCCCGCCAGACGCGCCCAGTATGAGGGAGATCG
TGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGG
CGACTCGGACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGAGCCATTCTGGTGGACCTGGAACCCGGA
ACCATGGACAGTGTCCGCTCAGGGGCCTTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGCACATCCAGCTGGTAGATGCCAACTCGCATCCAGCC
CCCAGCTGCCAGGGATTCTGGGGTGGCTGTTTGGGCTCCTGGCACTGCCAGTCGGAAGGAAGGTATGCTGGAGCAGGAGGGCTGATCCATTCACCCAGGA
TCCCCTCAGGAGGCAGAGCCTCGCTCTGCTGTGACCCCAAGTTCTCAAGACTCTGTCCAGAGCCCTCGTCCTGAGCACTCAGCAGCATCGGCTCAGGGAA
GCCACGGCGGGTCAGAGTGGGGCCGGCAACAACTGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAGG
AGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCG
TGAGGAGTATCCCGACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCCTACAACGCCACGCTGTCCATC
CACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTACG
GGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAA
CATGGTGCCCTTCCCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAGTACCGGGCCCTGACCGTGCCCGAGCTC
ACCCAGCAGATGTTCGATGCCAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGCATGTCCA
TGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACAT
CCCGCCCCGCGGCCTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTC
CGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACC
AGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGACGAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGAAGCTGCTCGCAGCT
GGAGTGAGAGGCAGGTGGCGGCCGGGGCCGAAGCCAGCAGTGTCTAAACCCCCGGAGCCATCTTGCTGCCGACACCCTGCTTTCCCCTCGCCCTAGGGCT
CCCTTGCCGCCCTCCTGCAGTATTTATGGCCTCGTCCTCCCCACCTAGGCCACGTGTGAGCTGCTCCTGTCTCTGTCTTATTGCAGCTCCAGGCCTGACG
TTTTACGGTTTTGTTTTTTACTGGTTTGTGTTTATATTTTCGGGGATACTTAATAAATCTATTGCTGTCAGATACCCTTG
  • TUBB3.u.f.17 (forward primer)
  • TUBB3.u.r.27 (reverse primer)
  • Single exon skipping

Frame 0

KSARPRSPTLSSQPGPPAPVRSRPPDAPSMREI
VHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVG
DSDLQLERISVYYNEASSHKYVPRAILVDLEPG
TMDSVRSGAFGHLFRPDNFIFAHPAGRCQLASS
PQLPGILGWLFGLLALPVGRKVCWSRRADPFTQD
PLRRQSLALL*PQVLKTLSRALVLSTQQHRLRE
ATAGQSGAGNNWAKGHYTEGAELVDSVLDVVRK
ECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVR
EEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSI
HQLVENTDETYCIDNEALYDICFRTLKLATPTY
GDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVN
MVPFPRLHFFMPGFAPLTARGSQQYRALTVPEL
TQQMFDAKNMMAACDPRHGRYLTVATVFRGRMS
MKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDI
PPRGLKMSSTFIGNSTAIQELFKRISEQFTAMF
RRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY
QQYQDATAEEEGEMYEDDEEESEAQGPK*SCSQL
E*EAGGGRGRSQQCLNPRSHLAADTLLSPRPRA
PLPPSCSIYGLVLPT*ATCELLLSLSYCSSRPD
VLRFCFLLVCVYIFGDT**IYCCQIPL

Frame 1

RARGRGPRPSAASPARPRPSAAARQTRPV*GRS
CTSRPASAATRSGPSSGKSSVMSMASTPAATTW
ATRTCSWSGSASTTTRPLLTSTCLEPFWWTWNPE
PWTVSAQGPLDISSGLTISSLHIQLVDANSHPA
PSCQGFWGGCLGSWHCQSEGRYAGAGGLIHSPR
IPSGGRASLCCDPKFSRLCPEPSS*ALSSIGSGK
PRRVRVGPATTGPRVTTRRGRSWWIRSWMWCGR
SVKTATACRASS*PTRWGAARAPAWARCSSARC
VRSIPTAS*TPSASCPHPRCQTRWWSPTTPRCPS
TSWWRTRMRPTASTTRRSTTSASAPSSWPRPPT
GTSTTWYRPP*AESPPPCASRASSTLTCASWPS
TWCPSRACTSSCPASPPSQPGAASSTGP*PCPSS
PSRCSMPRT*WPPATRATAAT*RWPPCSGAACP
*RRWTSRCWPSRARTAATSWSGSPTT*RWPCVT
SRPAASRCPPPSSGTARPSRSCSSASPSSSRPCS
GARPSCTGTRARAWTRWSSPRPRAT*TTWCPST
SSTRTPRPRKRARCTKTTRRSRRPRAPSEAARS
WSERQVAAGAEASSV*TPGAILLPTPCFPLALGL
PCRPPAVFMASSSPPRPRVSCSCLCLIAAPGLT
FYGFVFYWFVFIFSGILNKSIAVRYP

Frame 2

ERAAAVPDPQQPARPARARPQPPARRAQYEGDR
AHPGRPVRQPDRGQVLGSHQ**AWHRPQRQLRG
RLGLAAGADQRLLQRGLFSQVRASSHSGGPGTR
NHGQCPLRGLWTSLQA*QFHLCTSSW*MPTRIQP
PAARDSGVAVWAPGTASRKEGMLEQEG*SIHPG
SPQEAEPRSAVTPSSQDSVQSPRPEHSAASAQG
SHGGSEWGRQQLGQGSLHGGGGAGGFGPGCGAEG
V*KLRLPAGLPADPLAGGRHGLRHGHVAHQQGA
*GVSRPHHEHLQRRALTQGVRHGGGALQRHAVH
PPAGGEHG*DLLHRQRGALRHLLPHPQAGHAHLR
GPQPPGIGHHERSHHLLALPGPAQR*PAQAGRQ
HGALPAPALLHARLRPPHSPGQPAVPGPDRARA
HPADVRCQEHDGRLRPAPRPLPDGGHRVPGPHVH
EGGGRADAGHPEQEQQLLRGVDPQQREGGRV*H
PAPRPQDVLHLHREQHGHPGAVQAHLRAVHGHV
PAQGLPALVHGRGHGRDGVHRGREQHERPGVRVP
AVPGRHGRGRGRDVRRRRGGVGGPGPQVKLLAA
GVRGRWRPGPKPAVSKPPEPSCCRHPAFPSP*G
SLAALLQYLWPRPPHLGHV*AAPVSVLLQLQA*R
FTVLFFTGLCLYFRGYLINLLLSDTL

Copyright ©2008,2017, all rights reserved.