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Sequence LTA.fAug05
CGTCAGCACCCCAAGATGCATCTTGCCCACAGCACCCTCAAACCTGCTGCTCACCTCATTGGAGACCCCAGCAAGCAGAACTCACTGCTCTGGAGAGCAAACACGGACCGTGCCTTCCTCCAGGATGGTTTCTCCTTGAGCAACAATTCTCTCCTGGTCCCCACCAGTGGCATCTACTTCGTCTACTCCCAGGGGTCGTC
ACCACCTCTCCTTTGGCCATTCCAACAGCTCAAGTCTTCCCTGATCAAGTCACCGGAGCTTTCAAAGAAGGAATTCTAGGCATCCCAGGGGACCACACCT
CCCTGAACCATCCCTGATGTCTGTCTGGCTGAGGATTTCAAGCCTGCCTAGGAATTCCCAGCCCAAAGCTGTTGGTCTGTCCCACCAGCTAGGTGGGGCC
TAGATCCACACACAGAGGAAGAGCAGGCACATGGAGGAGCTTGGGGGATGACTAGAGGCAGGGAGGGGACTATTTATGAAGGCAAAAAAATTAAATTATT
TATTTATGGAGGATGGAGAGAGGGGAATAATAGAAGAACATCCAAGGAGAAACAGAGACAGGCCCAAGAGATGAAGAGTGAGAGGGCATGCGCACAAGGC
TGACCAAGAGAGAAAGAAGTAGGCATGAGGGATCACAGGGCCCCAGAAGGCAGGGAAAGGCTCTGAAAGCCAGCTGCCGACCAGAGCCCCACACGGAGGC
ATCTGCACCCTCGATGAAGCCCAATAAACCTCTTTTCTCTGA
- LTA.F6 (forward primer)
- LTA.R5 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
RQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQGSS
PPLLWPFQQLKSSLIKSPELSKKEF*ASQGTTP
P*TIPDVCLAEDFKPA*EFPAQSCWSVPPARWG
LDPHTEEEQAHGGAWGMTRGREGTIYEGKKIKLF
IYGGWREGNNRRTSKEKQRQAQEMKSERACAQG
*PREKEVGMRDHRAPEGRERL*KPAADQSPTRR
HLHPR*SPINLFSL
Frame 1
VSTPRCILPTAPSNLLLTSLETPASRTHCSGEQTRTVPSSRMVSP*ATILSWSPPVASTSSTPRGR
HHLSFGHSNSSSLP*SSHRSFQRRNSRHPRGPHL
PEPSLMSVWLRISSLPRNSQPKAVGLSHQLGGA
*IHTQRKSRHMEELGG*LEAGRGLFMKAKKLNY
LFMEDGERGIIEEHPRRNRDRPKR*RVRGHAHKA
DQERKK*A*GITGPQKAGKGSESQLPTRAPHGG
ICTLDEAQ*TSFL*
Frame 2
SAPQDASCPQHPQTCCSPHWRPQQAELTALESKHGPCLPPGWFLLEQQFSPGPHQWHLLRLLPGVV
TTSPLAIPTAQVFPDQVTGAFKEGILGIPGDHT
SLNHP*CLSG*GFQACLGIPSPKLLVCPTS*VGP
RSTHRGRAGTWRSLGDD*RQGGDYL*RQKN*II
YLWRMERGE**KNIQGETETGPRDEE*EGMRTR
LTKRERSRHEGSQGPRRQGKALKASCRPEPHTEA
SAPSMKPNKPLFS
Sequence LTA.gAug05
CGTCAGCACCCCAAGATGCATCTTGCCCACAGCACCCTCAAACCTGCTGCTCACCTCATTGCTCCCAGAAGATGGTGTATCCAGGGCTGCAGGAACCCTGGCTGCACTCGATGTACCACGGGGCTGCGTTCCAGCTCACCCAGGGAGACCAGCTATCCACCCACACAGATGGCATCCCCCACCTAGTCCTCAGCCCTAGT
ACTGTCTTCTTTGGAGCCTTCGCTCTGTAGAACTTGGAAAAATCCAGAAAGAAAAAATAATTGATTTCAAGACCTTCTCCCCATTCTGCCTCCATTCTGA
CCATTTCAGGGGTCGTCACCACCTCTCCTTTGGCCATTCCAACAGCTCAAGTCTTCCCTGATCAAGTCACCGGAGCTTTCAAAGAAGGAATTCTAGGCAT
CCCAGGGGACCACACCTCCCTGAACCATCCCTGATGTCTGTCTGGCTGAGGATTTCAAGCCTGCCTAGGAATTCCCAGCCCAAAGCTGTTGGTCTGTCCC
ACCAGCTAGGTGGGGCCTAGATCCACACACAGAGGAAGAGCAGGCACATGGAGGAGCTTGGGGGATGACTAGAGGCAGGGAGGGGACTATTTATGAAGGC
AAAAAAATTAAATTATTTATTTATGGAGGATGGAGAGAGGGGAATAATAGAAGAACATCCAAGGAGAAACAGAGACAGGCCCAAGAGATGAAGAGTGAGA
GGGCATGCGCACAAGGCTGACCAAGAGAGAAAGAAGTAGGCATGAGGGATCACAGGGCCCCAGAAGGCAGGGAAAGGCTCTGAAAGCCAGCTGCCGACCA
GAGCCCCACACGGAGGCATCTGCACCCTCGATGAAGCCCAATAAACCTCTTTTCTCTGA
- LTA.F6 (forward primer)
- LTA.R5 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
RQHPKMHLAHSTLKPAAHLIAPRRWCIQGCRNPGCTRCTTGLRSSSPRETSYPPTQMASPT*SSALV
LSSLEPSLCRTWKNPERKNN*FQDLLPILPPF*
PFQGSSPPLLWPFQQLKSSLIKSPELSKKEF*A
SQGTTPP*TIPDVCLAEDFKPA*EFPAQSCWSVP
PARWGLDPHTEEEQAHGGAWGMTRGREGTIYEG
KKIKLFIYGGWREGNNRRTSKEKQRQAQEMKSE
RACAQG*PREKEVGMRDHRAPEGRERL*KPAADQ
SPTRRHLHPR*SPINLFSL
Frame 1
VSTPRCILPTAPSNLLLTSLLPEDGVSRAAGTLAALDVPRGCVPAHPGRPAIHPHRWHPPPSPQP*
YCLLWSLRSVELGKIQKEKIIDFKTFSPFCLHSD
HFRGRHHLSFGHSNSSSLP*SSHRSFQRRNSRH
PRGPHLPEPSLMSVWLRISSLPRNSQPKAVGLS
HQLGGA*IHTQRKSRHMEELGG*LEAGRGLFMKA
KKLNYLFMEDGERGIIEEHPRRNRDRPKR*RVR
GHAHKADQERKK*A*GITGPQKAGKGSESQLPT
RAPHGGICTLDEAQ*TSFL*
Frame 2
SAPQDASCPQHPQTCCSPHCSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPS
TVFFGAFAL*NLEKSRKKK*LISRPSPHSASIL
TISGVVTTSPLAIPTAQVFPDQVTGAFKEGILGI
PGDHTSLNHP*CLSG*GFQACLGIPSPKLLVCP
TS*VGPRSTHRGRAGTWRSLGDD*RQGGDYL*R
QKN*IIYLWRMERGE**KNIQGETETGPRDEE*E
GMRTRLTKRERSRHEGSQGPRRQGKALKASCRP
EPHTEASAPSMKPNKPLFS
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