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Sequences

Sequence ALOX5.eAug05

CCACCAGACCATCACCCACCTTCTGCGAACACATCTGGTGTCTGAGGTTTTTGGCATTGCAATGTACCGCCAGCTGCCTGCTGTGCACCCCATTTTCAAG
CTGCTGGTGGCACACGTGAGATTCACCATTGCAATCAACACCAAGGCCCGTGAGCAGCTCATCTGCGAGTGTGGCCTCTTTGACAAGGCCATCAAGGCCC
GGGGCATGGAGAGCAAAGAAGACATCCCCTACTACTTCTACCGGGACGACGGGCTCCTGGTGTGGGAAGCCATCAGGACGTTCACGGCCGAGGTGGTAGA
CATCTACTACGAGGGCGACCAGGTGGTGGAGGAGGACCCGGAGCTGCAGGACTTCGTGAACGATGTCTACGTGTACGGCATGCGGGGCCGCAAGTCCTCA
GGCTTCCCCAAGTCGGTCAAGAGCCGGGAGCAGCTGTCGGAGTACCTGACCGTGGTGATCTTCACCGCCTCCGCCCAGCACGCCGCGGTCAACTTCGGCC
AGTACGACTGGTGCTCCTGGATCCCCAATGCGCCCCCAACCATGCGAGCCCCGCCACCGACTGCCAAGGGCGTGGTGACCATTGAGCAGATCGTGGACAC
GCTGCCCGACCGCGGCCGCTCCTGCTGGCATCTGGGTGCAGTGTGGGCGCTGAGCCAGTTCCAGGAAAACGAGGTGAAGCTGGGCAGGGCGGGGCACAGC
CCCAGGTCACCCCAGGTTAAGCGGTTCCTCAGCCTCAGGGCTTTGTGACTCGGGCCCCAAGGCTCACTTGGAGCAAAGGAATCCTGACTTCCAAGGCTGG
AAGGGCCCAGAAGGCTGCAGCCGCCACCAGGTCCCCCGGCCTCAGCCTGGACAGAGCTCAGGGTGTGCAGGGCAGGAGAGCACACAGCCCAGGCTTTGCT
CACTGTCACCAGAGGGTCGTGTGTGACGCCCCCTCCCCCCAGCTATTGACAAAGTTCTTGCACATGTGTTTTACCCTGGTACTCCAGAGGGAATGACCAA
GAGTTTCCTGGGTTCCTTCCGGACACGTGCATCTCATTTAACCTAACAACTGAATCCGGTGTGTCTTCGTGGATGCACCCGCTTTGGGGTAGCTCAGTAC
TGCATAGAATGGCCGGACCAGTCCAACCTGCTCCAGACTGCTGGGTGTCTGAGTTGTTCCCGGCCAATGTGTTGGTCCCTCACTGGACATCCTTGTTTTT
GTGGCTTTGCACATGTGTTCAGTATGACCGCACACATTCCTAGCAGACAAATTCCTTGGTCAAAGAATATTGATACTGAATTGCCCTCCACAAACTAAAT
TCAAAATCCCATTATCAATAGCCTATGTTTCTTGGACCTTTCCAGCTCAGAAGCTTTGTGATCTTAATCCAATACAGTATTTTTTTAAAATGATATCTAG
CTCTGCCCCGCAGACATCTGTGTGAGAATGCAAATAAGCACAGGACCCCAACAGGGCAGCCACCCCTTCAGGCTCCCTGGCCCGCTTTCTGCCTTCCTGG
GCTGGAGAGGCCAGTGCTGGCCCCAGCGCCCCTGATGGGAGGTGAGAGTGCTGCGCAGGGGTGGCCCAAGACAGCAGGATACCATGGCTGCAAACACCAG
CAGCCCCCAGCTTCATCTGCATCATCTCGTAACCACTTGGCAGCAGGCAGTTATTTTCCCACTTATCCATGAAGCCCCAGCCCTGGAGCCTTCCTTAGAG
AAGCAGGTTGGAGGCGACGACACTTGCCTTCCCGAGGCCCTCTTGTCAGGCAGCAGAGGGTGAATATGGGGAGGTGAATAGATGCTCCCTCCTTCATCTC
CCAAACGGTGGCTGGCCCCTTGGGATGAGACAGGCCTGTCAGTTTACACGGGTAGTGGATTGACCTATGTGTGTGTCCATGTCTGGGCCCTCAGCTGTTC
CTGGGCATGTACCCAGAAGAGCATTTTATCGAGAAGCCTGTGAAGGAAGCCATGGCCCGATTCCGCAAGAACCTCGAGGCCATTGTCAGCGTGATTGCTG
AGCGCAACAAGAAGAAGCAGCTGCCATATTAC
  • ALOX5.F16 (forward primer)
  • ALOX5.R7 (reverse primer)
  • Alternative acceptor

Frame 0

PPDHHPPSANTSGV*GFWHCNVPPAACCAPHFQ
AAGGTREIHHCNQHQGP*AAHLRVWPL*QGHQGP
GHGEQRRHPLLLLPGRRAPGVGSHQDVHGRGGR
HLLRGRPGGGGGPGAAGLRERCLRVRHAGPQVL
RLPQVGQEPGAAVGVPDRGDLHRLRPARRGQLRP
VRLVLLDPQCAPNHASPATDCQGRGDH*ADRGH
AARPRPLLLASGCSVGAEPVPGKRGEAGQGGAQ
PQVTPG*AVPQPQGFVTRAPRLTWSKGILTSKAG
RAQKAAAATRSPGLSLDRAQGVQGRRAHSPGFA
HCHQRVVCDAPSPQLLTKFLHMCFTLVLQRE*P
RVSWVPSGHVHLI*PNN*IRCVFVDAPALG*LST
A*NGRTSPTCSRLLGV*VVPGQCVGPSLDILVF
VALHMCSV*PHTFLADKFLGQRILILNCPPQTK
FKIPLSIAYVSWTFPAQKLCDLNPIQYFFKMISS
SAPQTSV*ECK*AQDPNRAATPSGSLARFLPSW
AGEASAGPSAPDGR*ECCAGVAQDSRIPWLQTP
AAPSFICIIS*PLGSRQLFSHLSMKPQPWSLP*R
SRLEATTLAFPRPSCQAAEGEYGEVNRCSLLHL
PNGGWPLGMRQACQFTRVVD*PMCVSMSGPSAV
PGHVPRRAFYREACEGSHGPIPQEPRGHCQRDC*
AQQEEAAAIL

Frame 1

HQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFK
LLVAHVRFTIAINTKAREQLICECGLFDKAIKA
RGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVD
IYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSS
GFPKSVKSREQLSEYLTVVIFTASAQHAAVNFG
QYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDT
LPDRGRSCWHLGAVWALSQFQENEVKLGRAGHS
PRSPQVKRFLSLRAL*LGPQGSLGAKES*LPRL
EGPRRLQPPPGPPASAWTELRVCRAGEHTAQALL
TVTRGSCVTPPPPSY*QSSCTCVLPWYSRGNDQ
EFPGFLPDTCISFNLTTESGVSSWMHPLWGSSV
LHRMAGPVQPAPDCWVSELFPANVLVPHWTSLFL
WLCTCVQYDRTHS*QTNSLVKEY*Y*IALHKLN
SKSHYQ*PMFLGPFQLRSFVILIQYSIFLK*YL
ALPRRHLCENANKHRTPTGQPPLQAPWPAFCLPG
LERPVLAPAPLMGGESAAQGWPKTAGYHGCKHQ
QPPASSASSRNHLAAGSYFPTYP*SPSPGAFLR
EAGWRRRHLPSRGPLVRQQRVNMGR*IDAPSFIS
QTVAGPLG*DRPVSLHG*WIDLCVCPCLGPQLF
LGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIA
ERNKKKQLPYY

Frame 2

TRPSPTFCEHIWCLRFLALQCTASCLLCTPFSS
CWWHT*DSPLQSTPRPVSSSSASVASLTRPSRP
GAWRAKKTSPTTSTGTTGSWCGKPSGRSRPRW*
TSTTRATRWWRRTRSCRTS*TMSTCTACGAASPQ
ASPSRSRAGSSCRST*PW*SSPPPPSTPRSTSA
STTGAPGSPMRPQPCEPRHRLPRAW*PLSRSWT
RCPTAAAPAGIWVQCGR*ASSRKTR*SWAGRGTA
PGHPRLSGSSASGLCDSGPKAHLEQRNPDFQGW
KGPEGCSRHQVPRPQPGQSSGCAGQESTQPRLC
SLSPEGRV*RPLPPAIDKVLAHVFYPGTPEGMTK
SFLGSFRTRASHLT*QLNPVCLRGCTRFGVAQY
CIEWPDQSNLLQTAGCLSCSRPMCWSLTGHPCF
CGFAHVFSMTAHIPSRQIPWSKNIDTELPSTN*I
QNPIINSLCFLDLSSSEAL*S*SNTVFF*NDI*
LCPADICVRMQISTGPQQGSHPFRLPGPLSAFL
GWRGQCWPQRP*WEVRVLRRGGPRQQDTMAANTS
SPQLHLHHLVTTWQQAVIFPLIHEAPALEPSLE
KQVGGDDTCLPEALLSGSRG*IWGGE*MLPPSS
PKRWLAPWDETGLSVYTGSGLTYVCVHVWALSCS
WACTQKSILSRSL*RKPWPDSARTSRPLSA*LL
SATRRSSCHI

Sequence ALOX5.aAug05

CCGGGGCCAGGGACCAGTGGTGGGAGGAGGCTGCGGCGCTAGATGCGGACACCTGGACCGCCGCGCCGAGGCTCCCGGCGCTCGCTGCTCCCGCGGCCCG
CGCCATGCCCTCCTACACGGTCACCGTGGCCACTGGCAGCCAGTGGTTCGCCGGCACTGACGACTACATCTACCTCAGCCTCGTGGGCTCGGCGGGCTGC
AGCGAGAAGCACCTGCTGGACAAGCCCTTCTACAACGACTTCGAGCGTGGCGCGGTGGATTCATACGACGTGACTGTGGACGAGGAACTGGGCGAGATCC
AGCTGGTCAGAATCGAGAAGCGCAAGTACTGGCTGAATGACGACTGGTACCTGAAGTACATCACGCTGAAGACGCCCCACGGGGACTACATCGAGTTCCC
CTGCTACCGCTGGATCACCGGCGATGTCGAGGTTGTCCTGAGGGATGGACGCGCAAAGTTGGCCCGAGATGACCAAATTCACATTCTCAAGCAACACCGA
CGTAAAGAACTGGAAACACGGCAAAAACAATATCGATGGATGGAGTGGAACCCTGGCTTCCCCTTGAGCATCGATGCCAAATGCCACAAGGATTTACCCC
GTGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTTTGTTCTGAATTACTCCAAAGCGATGGAGAACCTGTTCATCAACCGCTTCATGCACATGTTCCA
GTCTTCTTGGAATGACTTCGCCGACTTTGAGAAAATCTTTGTCAAGATCAGCAACACTATTTCTGAGCGGGTCATGAATCACTGGCAGGAAGACCTGATG
TTTGGCTACCAGTTCCTGAATGGCTGCAACCCTGTGTTGATCCGGCGCTGCACAGAGCTGCCCGAGAAGCTCCCGGTGACCACGGAGATGGTAGAGTGCA
GCCTGGAGCGGCAGCTCAGCTTGGAGCAGGAGGTCCAGCAAGGGAACATTTTCATCGTGGACTTTGAGCTGCTGGATGGCATCGATGCCAACAAAACAGA
CCCCTGCACACTCCAGTTCCTGGCCGCTCCCATCTGCTTGCTGTATAAGAACCTGGCCAACAAGATTGTCCCCATTGCCATCCAGCTCAACCAAATCCCG
GGAGATGAGAACCCTATTTTCCTCCCTTCGGATGCAAAATACGACTGGCTTTTGGCCAAAATCTGGGTGCGTTCCAGTGACTTCCACGTCCACCAGACCA
TCACCCACCTTCTGCGAACACATCTGGTGTCTGAGGTTTTTGGCATTGCAATGTACCGCCAGCTGCCTGCTGTGCACCCCATTTTCAAGCTGCTGGTGGC
ACACGTGAGATTCACCATTGCAATCAACACCAAGGCCCGTGAGCAGCTCATCTGCGAGTGTGGCCTCTTTGACAAGGCCAACGCCACAGGGGGCGGTGGG
CACGTGCAGATGGTGCAGAGGGCCATGAAGGACCTGACCTATGCCTCCCTGTGCTTTCCCGAGGCCATCAAGGCCCGGGGCATGGAGAGCAAAGAAGACA
TCCCCTACTACTTCTACCGGGACGACGGGCTCCTGGTGTGGGAAGCCATCAGGACGTTCACGGCCGAGGTGGTAGACATCTACTACGAGGGCGACCAGGT
GGTGGAGGAGGACCCGGAGCTGCAGGACTTCGTGAACGATGTCTACGTGTACGGCATGCGGGGCCGCAAGTCCTCAGGCTTCCCCAAGTCGGTCAAGAGC
CGGGAGCAGCTGTCGGAGTACCTGACCGTGGTGATCTTCACCGCCTCCGCCCAGCACGCCGCGGTCAACTTCGGCCAGTACGACTGGTGCTCCTGGATCC
CCAATGCGCCCCCAACCATGCGAGCCCCGCCACCGACTGCCAAGGGCGTGGTGACCATTGAGCAGATCGTGGACACGCTGCCCGACCGCGGCCGCTCCTG
CTGGCATCTGGGTGCAGTGTGGGCGCTGAGCCAGTTCCAGGAAAACGAGCTGTTCCTGGGCATGTACCCAGAAGAGCATTTTATCGAGAAGCCTGTGAAG
GAAGCCATGGCCCGATTCCGCAAGAACCTCGAGGCCATTGTCAGCGTGATTGCTGAGCGCAACAAGAAGAAGCAGCTGCCATATTACTACTTGTCCCCAG
ACCGGATTCCGAACAGTGTGGCCATCTGAGCACACTGCCAGTCTCACTGTGGGAAGGCCAGCTGCCCCAGCCAGATGGACTCCAGCCTGCCTGGCAGGCT
GTCTGGCCAGGCCTCTTGGCAGTCACATCTCTTCCTCCGAGGCCAGTACCTTTCCATTTATTCTTTGATCTTCAGGGAACTGCATAGATTGATCAAAGTG
TAAACACCATAGGGACCCATTCTACACAGAGCAGGACTGCACAGCGTCCTGTCCACACCCAGCTCAGCATTTCCACACCAAGCAGCAACAGCAAATCACG
ACCACTGATAGATGTCTATTCTTGTTGGAGACATGGGATGATTATTTTCTGTTCTATTTGTGCTTAGTCCAATTCCTTGCACATAGTAGGTACCCAATTC
AATTACTATTGAATGAATTAAGAATTGGTTGCCATAAAAATAAATCAGTTCATTTAAAATG
  • ALOX5.F16 (forward primer)
  • ALOX5.R7 (reverse primer)
  • Alternative acceptor

Frame 0

PGPGTSGGRRLRR*MRTPGPPRRGSRRSLLPRP
APCPPTRSPWPLAASGSPALTTTSTSASWARRAA
ARSTCWTSPSTTTSSVARWIHTT*LWTRNWARS
SWSESRSASTG*MTTGT*STSR*RRPTGTTSSS
PATAGSPAMSRLS*GMDAQSWPEMTKFTFSSNTD
VKNWKHGKNNIDGWSGTLASP*ASMPNATRIYP
VISSLIVKKEWTLF*ITPKRWRTCSSTASCTCS
SLLGMTSPTLRKSLSRSATLFLSGS*ITGRKT*C
LATSS*MAATLC*SGAAQSCPRSSR*PRRW*SA
AWSGSSAWSRRSSKGTFSSWTLSCWMASMPTKQ
TPAHSSSWPLPSACCIRTWPTRLSPLPSSSTKSR
EMRTLFSSLRMQNTTGFWPKSGCVPVTSTSTRP
SPTFCEHIWCLRFLALQCTASCLLCTPFSSCWW
HT*DSPLQSTPRPVSSSSASVASLTRPTPQGAVG
TCRWCRGP*RT*PMPPCAFPRPSRPGAWRAKKT
SPTTSTGTTGSWCGKPSGRSRPRW*TSTTRATR
WWRRTRSCRTS*TMSTCTACGAASPQASPSRSRA
GSSCRST*PW*SSPPPPSTPRSTSASTTGAPGS
PMRPQPCEPRHRLPRAW*PLSRSWTRCPTAAAP
AGIWVQCGR*ASSRKTSCSWACTQKSILSRSL*R
KPWPDSARTSRPLSA*LLSATRRSSCHITTCPQ
TGFRTVWPSEHTASLTVGRPAAPARWTPACLAG
CLARPLGSHISSSEASTFPFIL*SSGNCID*SKC
KHHRDPFYTEQDCTASCPHPAQHFHTKQQQQIT
TTDRCLFLLETWDDYFLFYLCLVQFLAHSRYPI
QLLLNELRIGCHKNKSVHLK

Frame 1

RGQGPVVGGGCGARCGHLDRRAEAPGARCSRGP
RHALLHGHRGHWQPVVRRH*RLHLPQPRGLGGL
QREAPAGQALLQRLRAWRGGFIRRDCGRGTGRDP
AGQNREAQVLAE*RLVPEVHHAEDAPRGLHRVP
LLPLDHRRCRGCPEGWTRKVGPR*PNSHSQATP
T*RTGNTAKTISMDGVEPWLPLEHRCQMPQGFTP
*YPV***KRSGLCSELLQSDGEPVHQPLHAHVP
VFLE*LRRL*ENLCQDQQHYF*AGHESLAGRPD
VWLPVPEWLQPCVDPALHRAAREAPGDHGDGRVQ
PGAAAQLGAGGPAREHFHRGL*AAGWHRCQQNR
PLHTPVPGRSHLLAV*EPGQQDCPHCHPAQPNP
GR*EPYFPPFGCKIRLAFGQNLGAFQ*LPRPPDH
HPPSANTSGV*GFWHCNVPPAACCAPHFQAAGG
TREIHHCNQHQGP*AAHLRVWPL*QGQRHRGRW
ARADGAEGHEGPDLCLPVLSRGHQGPGHGEQRRH
PLLLLPGRRAPGVGSHQDVHGRGGRHLLRGRPG
GGGGPGAAGLRERCLRVRHAGPQVLRLPQVGQE
PGAAVGVPDRGDLHRLRPARRGQLRPVRLVLLDP
QCAPNHASPATDCQGRGDH*ADRGHAARPRPLL
LASGCSVGAEPVPGKRAVPGHVPRRAFYREACE
GSHGPIPQEPRGHCQRDC*AQQEEAAAILLLVPR
PDSEQCGHLSTLPVSLWEGQLPQPDGLQPAWQA
VWPGLLAVTSLPPRPVPFHLFFDLQGTA*IDQS
VNTIGTHSTQSRTAQRPVHTQLSISTPSSNSKSR
PLIDVYSCWRHGMIIFCSICA*SNSLHIVGTQF
NYY*MN*ELVAIKINQFI*N

Frame 2

GARDQWWEEAAALDADTWTAAPRLPALAAPAAR
AMPSYTVTVATGSQWFAGTDDYIYLSLVGSAGC
SEKHLLDKPFYNDFERGAVDSYDVTVDEELGEI
QLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFP
CYRWITGDVEVVLRDGRAKLARDDQIHILKQHR
RKELETRQKQYRWMEWNPGFPLSIDAKCHKDLP
RDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQ
SSWNDFADFEKIFVKISNTISERVMNHWQEDLM
FGYQFLNGCNPVLIRRCTELPEKLPVTTEMVEC
SLERQLSLEQEVQQGNIFIVDFELLDGIDANKTD
PCTLQFLAAPICLLYKNLANKIVPIAIQLNQIP
GDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQT
ITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVA
HVRFTIAINTKAREQLICECGLFDKANATGGGG
HVQMVQRAMKDLTYASLCFPEAIKARGMESKED
IPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQV
VEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKS
REQLSEYLTVVIFTASAQHAAVNFGQYDWCSWI
PNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSC
WHLGAVWALSQFQENELFLGMYPEEHFIEKPVK
EAMARFRKNLEAIVSVIAERNKKKQLPYYYLSP
DRIPNSVAI*AHCQSHCGKASCPSQMDSSLPGRL
SGQASWQSHLFLRGQYLSIYSLIFRELHRLIKV
*TP*GPILHRAGLHSVLSTPSSAFPHQAATANH
DH**MSILVGDMG*LFSVLFVLSPIPCT**VPNS
ITIE*IKNWLP*K*ISSFKM

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