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Sequence KLK8.bAug05
CATGGAGGAGGAAGGAGATGGCATGGCTTACCATAAAGAAGCACTGGACGCCGGGTGCACGTTCCAGGATCCAGGTGCCCAGGGGTCATGAAGCTGGGACTCCTCTGTGCTCTGCTCTCTCTGCTGGCAGGGCATGGCTGGGCAGACACCCGTGCCATCGGGGCCGAGGAATGTCGCCCCAACTCCCAGCCTTGGCAGGC
CGGCCTCTTCCACCTTACTCGGCTCTTCTGTGGGGCGACCCTCATCAGTGACCGCTGGCTGCTCACAGCTGCCCACTGCCGCAAGCCGTATCTGTGGGTC
CGCCTTGGAGAGCACCACCTCTGGAAATGGGAGGGTCCGGAGCAGCTGTTCCGGGTTACGGACTTCTTCCCCCACCCTGGCTTCAACAAGGACCTCAGCG
CCAATGACCACAATGATGACATCATGCTGATCCGCCTGCCCAGGCAGGCACGTCTGAGTCCTGCTGTGCAGCCCCTCAACCTCAGCCAGACCTGTGTCTC
CCCAGGCATGCAGTGTCTCATCTCAGGCTGGGGGGCCGTGTCCAGCCCCAAGGCGCTGTTTCCAGTCACACTGCAGTGTGCCAACATCAGCATCCTGGAG
AACAAACTCTGTCACTGGGCATACCCTGGACACATCTCGGACAGCATGCTCTGTGCGGGCCTGTGGGAGGGGGGCCGAGGTTCCTGCCAGGGTGACTCTG
GGGGCCCCCTGGTTTGCAATGGAACCTTGGCAGGCGTGGTGTCTGGGGGTGCTGAGCCCTGCTCCAGACCCCGGCGCCCCGCAGTCTACACCAGCGTATG
CCACTACCTTGACTGGATCCAAGAAATCATGGAGAACTGAGCCCGCGCGCCACGGGGGCACCTTGGAAGACCAAGAGAGGCCGAAGGGCACGGGGTAGGG
GGTTCTCGTAGGGTCCCAGCCTCAATGGTTCCCGCCCTGGACCTCCAGCTGCCCTGACTCCCCTCTGGACACTAAGACTCCGCCCCTGAGGCTCCGCCCC
CTCACGAGGTCAAGCAAGACACAGTCGCGCCCCCTCGGAACGGAGCAGGGACACGCCCTTCAGAGCCCGTCTCTATGACGTCACCGACAGCCATCACCTC
CTTCTTGGAACAGCACAGCCTGTGGCTCCGCCCCAAGGAACCACTTACACAAAATAGCTCCGCCCCTCGGAACTTTGCCCAGTGGGACTTCCCCTCGGGA
CTCCACCCCTTGTGGCCCCGCCTCCTTCACCAGAGATCTCGCCCCTCGTGATGTCAGGGGCGCAGTAGCTCCGCCCACGTGGAGCTCGGGCGGTGTAGAG
CTCAGCCCCTTGTGGCCCCGTCCTGGGCGTGTGCTGGGTTTGAATCCTGGCGGAGACCTGGGGGGAAATTGAGGGAGGGTCTGGATACCTTTAGAGCCAA
TGCAACGGATGATTTTTCAGTAAACGCGGGAAACCTCACCTTCTTTCTGCCTGAGCTGTGAGATGAGTGGAGAGCAAACGGGTGGGCGGTGAAGGGCAGA
TGAGGGAACCGGTACCGCCTTGCAACTCCCCCTTAAACCCCTATGTTCCAGTTCCCAGAAGCTCCCCAGGCTCTAGTGCAGGAGGAGAAGGAGGAGGAGC
AGGAGGTGGAGATTCCCAGTTAAAAGGCTCCAGAATCGTGTACCAGGCAGAGAACTGAAGTACTGGGGCCTCCTCCACTGGGTCCGAATCAGTAGGTGAC
CCCGCCCCTGGATTCTGGAAGACCTCACCATGGGACGCCCCCGACCTCGTGCGGCCAAGACGTGGATGTTCCTGCTCTTGCTGGGGGGAGCCTGGGCAGG
ACACTCCAGGGCACAGGAGGACAAGGTGCTGGGGGGTCATGAGTGCCAACCCCATTCGCAGCCTTGGCAGGCGGCCTTGTTCCAGGGCCAGCAACTACTC
TGTGGCGGTGTCCTTGTAGGTGGCAACTGGGTCCTTACAGCTGCCCACTGTAAAAAACCGAAATACACAGTACGCCTGGGAGACCACAGCCTACAGAATA
AAGATGGCCCAGAGCAAGAAATACCTGTGGTTCAGTCCATCCCACACCCCTGCTACAACAGCAGCGATGTGGAGGACCACAACCATGATCTGATGCTTCT
TCAACTGCGTGACCAGGCATCCCTGGGGTCCAAAGTGAAGCCCATCAGCCTGGCAGATCATTGCACCCAGCCTGGCCAGAAGTGCACCGTCTCAGGCTGG
GGCACTGTCACCAGTCCCCGAGAGAATTTTCCTGACACTCTCAACTGTGCAGAAGTAAAAATCTTTCCCCAGAAGAAGTGTGAGGATGCTTACCCGGGGC
AGATCACAGATGGCATGGTCTGTGCAGGCAGCAGCAAAGGGGCTGACACGTGCCAGGGCGATTCTGGAGGCCCCCTGGTGTGTGATGGTGCACTCCAGGG
CATCACATCCTGGGGCTCAGACCCCTGTGGGAGGTCCGACAAACCTGGCGTCTATACCAACATCTGCCGCTACCTGGACTGGATCAAGAAGATCATAGGC
AGCAAGGGCTGATTCTAGGATAAGCACTAGATCTCCCTTAATAAACTCACAACTCTCTGGTTCCTTGCCTGTCTCTGTTTTGGCCCTGTGGGGAGGGCTG
GATGGGGATCCGGGATTGTTCCTGCTGGCAGACTAGGTGGGGATGTGCAGAAACCAAGTTCTCATGGTCACTTTGGCCATCAC
- KLK8.F1 (forward primer)
- KLK8.R13 (reverse primer)
- Alternative acceptor
Frame 0
HGGGRRWHGLP*RSTGRRVHVPGSRCPGVMKLGLLCALLSLLAGHGWADTRAIGAEECRPNSQPWQA
GLFHLTRLFCGATLISDRWLLTAAHCRKPYLWV
RLGEHHLWKWEGPEQLFRVTDFFPHPGFNKDLS
ANDHNDDIMLIRLPRQARLSPAVQPLNLSQTCVS
PGMQCLISGWGAVSSPKALFPVTLQCANISILE
NKLCHWAYPGHISDSMLCAGLWEGGRGSCQGDS
GGPLVCNGTLAGVVSGGAEPCSRPRRPAVYTSVC
HYLDWIQEIMEN*ARAPRGHLGRPREAEGHGVG
GSRRVPASMVPALDLQLP*LPSGH*DSAPEAPP
PHEVKQDTVAPPRNGAGTRPSEPVSMTSPTAITS
FLEQHSLWLRPKEPLTQNSSAPRNFAQWDFPSG
LHPLWPRLLHQRSRPS*CQGRSSSAHVELGRCR
AQPLVAPSWACAGFESWRRPGGKLREGLDTFRAN
ATDDFSVNAGNLTFFLPEL*DEWRANGWAVKGR
*GNRYRLATPP*TPMFQFPEAPQALVQEEKEEE
QEVEIPS*KAPESCTRQRTEVLGPPPLGPNQ*VT
PPLDSGRPHHGTPPTSCGQDVDVPALAGGSLGR
TLQGTGGQGAGGS*VPTPFAALAGGLVPGPATT
LWRCPCRWQLGPYSCPL*KTEIHSTPGRPQPTE*
RWPRARNTCGSVHPTPLLQQQRCGGPQP*SDAS
STA*PGIPGVQSEAHQPGRSLHPAWPEVHRLRL
GHCHQSPREFS*HSQLCRSKNLSPEEV*GCLPGA
DHRWHGLCRQQQRG*HVPGRFWRPPGV*WCTPG
HHILGLRPLWEVRQTWRLYQHLPLPGLDQEDHR
QQGLILG*ALDLP**THNSLVPCLSLFWPCGEGW
MGIRDCSCWQTRWGCAETKFSWSLWPS
Frame 1
MEEEGDGMAYHKEALDAGCTFQDPGAQGS*SWDSSVLCSLCWQGMAGQTPVPSGPRNVAPTPSLGR
PASSTLLGSSVGRPSSVTAGCSQLPTAASRICGS
ALESTTSGNGRVRSSCSGLRTSSPTLASTRTSA
PMTTMMTSC*SACPGRHV*VLLCSPSTSARPVS
PQACSVSSQAGGPCPAPRRCFQSHCSVPTSASWR
TNSVTGHTLDTSRTACSVRACGRGAEVPARVTL
GAPWFAMEPWQAWCLGVLSPAPDPGAPQSTPAY
ATTLTGSKKSWRTEPARHGGTLEDQERPKGTG*G
VLVGSQPQWFPPWTSSCPDSPLDTKTPPLRLRP
LTRSSKTQSRPLGTEQGHALQSPSL*RHRQPSP
PSWNSTACGSAPRNHLHKIAPPLGTLPSGTSPRD
STPCGPASFTRDLAPRDVRGAVAPPTWSSGGVE
LSPLWPRPGRVLGLNPGGDLGGN*GRVWIPLEP
MQRMIFQ*TRETSPSFCLSCEMSGEQTGGR*RAD
EGTGTALQLPLKPLCSSSQKLPRL*CRRRRRRS
RRWRFPVKRLQNRVPGRELKYWGLLHWVRISR*
PRPWILEDLTMGRPRPRAAKTWMFLLLLGGAWAG
HSRAQEDKVLGGHECQPHSQPWQAALFQGQQLL
CGGVLVGGNWVLTAAHCKKPKYTVRLGDHSLQN
KDGPEQEIPVVQSIPHPCYNSSDVEDHNHDLMLL
QLRDQASLGSKVKPISLADHCTQPGQKCTVSGW
GTVTSPRENFPDTLNCAEVKIFPQKKCEDAYPG
QITDGMVCAGSSKGADTCQGDSGGPLVCDGALQG
ITSWGSDPCGRSDKPGVYTNICRYLDWIKKIIG
SKG*F*DKH*ISLNKLTTLWFLACLCFGPVGRA
GWGSGIVPAGRLGGDVQKPSSHGHFGHH
Frame 2
WRRKEMAWLTIKKHWTPGARSRIQVPRGHEAGTPLCSALSAGRAWLGRHPCHRGRGMSPQLPALAG
RPLPPYSALLWGDPHQ*PLAAHSCPLPQAVSVG
PPWRAPPLEMGGSGAAVPGYGLLPPPWLQQGPQR
Q*PQ**HHADPPAQAGTSESCCAAPQPQPDLCL
PRHAVSHLRLGGRVQPQGAVSSHTAVCQHQHPG
EQTLSLGIPWTHLGQHALCGPVGGGPRFLPG*LW
GPPGLQWNLGRRGVWGC*ALLQTPAPRSLHQRM
PLP*LDPRNHGELSPRATGAPWKTKRGRRARGR
GFS*GPSLNGSRPGPPAALTPLWTLRLRP*GSAP
SRGQARHSRAPSERSRDTPFRARLYDVTDSHHL
LLGTAQPVAPPQGTTYTK*LRPSELCPVGLPLG
TPPLVAPPPSPEISPLVMSGAQ*LRPRGARAV*S
SAPCGPVLGVCWV*ILAETWGEIEGGSGYL*SQ
CNG*FFSKRGKPHLLSA*AVR*VESKRVGGEGQ
MREPVPPCNSPLNPYVPVPRSSPGSSAGGEGGGA
GGGDSQLKGSRIVYQAEN*STGASSTGSESVGD
PAPGFWKTSPWDAPDLVRPRRGCSCSCWGEPGQ
DTPGHRRTRCWGVMSANPIRSLGRRPCSRASNYS
VAVSL*VATGSLQLPTVKNRNTQYAWETTAYRI
KMAQSKKYLWFSPSHTPATTAAMWRTTTMI*CF
FNCVTRHPWGPK*SPSAWQIIAPSLARSAPSQAG
ALSPVPERIFLTLSTVQK*KSFPRRSVRMLTRG
RSQMAWSVQAAAKGLTRARAILEAPWCVMVHSR
ASHPGAQTPVGGPTNLASIPTSAATWTGSRRS*A
ARADSRISTRSPLINSQLSGSLPVSVLALWGGL
DGDPGLFLLAD*VGMCRNQVLMVTLAI
Sequence KLK8.dAug05
CTCTCCCTAATTTAGGAGTCCCAGCTCCCAGCTCCCTGTCCCCTCAGACCCAGACATCGAGGACTCCCCCCTCCCTTGGAATGTAGGAATCCAGTCCCCCAGCCTCCTCCTTCCTCCAGAGAAGCCCAGAACAGCCCCAGATACTCTCGGCTGCCTCCCCAGTGCCCAAATCCAGAACTGGGAGCTCAGGCTCCTCCTTC
CTGTTTACCGGCCCCGCCCTCTCCATTTCCCAGACCTCACCATGGGACGCCCCCGACCTCGTGCGGCCAAGACGTGGATGTTCCTGCTCTTGCTGGGGGG
AGCCTGGGCAGGACACTCCAGGGCACAGGAGGACAAGGTGCTGGGGGGTCATGAGTGCCAACCCCATTCGCAGCCTTGGCAGGCGGCCTTGTTCCAGGGC
CAGCAACTACTCTGTGGCGGTGTCCTTGTAGGTGGCAACTGGGTCCTTACAGCTGCCCACTGTAAAAAACCGAAATACACAGTACGCCTGGGAGACCACA
GCCTACAGAATAAAGATGGCCCAGAGCAAGAAATACCTGTGGTTCAGTCCATCCCACACCCCTGCTACAACAGCAGCGATGTGGAGGACCACAACCATGA
TCTGATGCTTCTTCAACTGCGTGACCAGGCATCCCTGGGGTCCAAAGTGAAGCCCATCAGCCTGGCAGATCATTGCACCCAGCCTGGCCAGAAGTGCACC
GTCTCAGGCTGGGGCACTGTCACCAGTCCCCGAGAGAATTTTCCTGACACTCTCAACTGTGCAGAAGTAAAAATCTTTCCCCAGAAGAAGTGTGAGGATG
CTTACCCGGGGCAGATCACAGATGGCATGGTCTGTGCAGGCAGCAGCAAAGGGGCTGACACGTGCCAGTAGAGACAGGGATTCACCATGTTGGCCAGGCT
GGTCTGGAACGCCTGACTTCAAGTGATCTACCGCCTCGGCCTCCCAAAGTGCCGGGATTGCAGGGCGATTCTGGAGGCCCCCTGGTGTGTGATGGTGCAC
TCCAGGGCATCACATCCTGGGGCTCAGACCCCTGTGGGAGGTCCGACAAACCTGGCGTCTATACCAACATCTGCCGCTACCTGGACTGGATCAAGAAGAT
CATAGGCAGCAAGGGCTGATTCTAGGATAAGCACTAGATCTCCCTTAATAAACTCACAACTCTCTGGTTCCTTGCCTGTCTCTGTTTTGGCCCTGTGGGG
AGGGCTGGATGGGGATCCGGGATTGTTCCTGCTGGCAGACTAGGTGGGGATGTGCAGAAACCAAGTTCTCATGGTCACTTTGGCCATCAC
- KLK8.F1 (forward primer)
- KLK8.R13 (reverse primer)
- Alternative acceptor
Frame 0
LSLI*ESQLPAPCPLRPRHRGLPPPLECRNPVPQPPPSSREAQNSPRYSRLPPQCPNPELGAQAPPS
CLPAPPSPFPRPHHGTPPTSCGQDVDVPALAGG
SLGRTLQGTGGQGAGGS*VPTPFAALAGGLVPG
PATTLWRCPCRWQLGPYSCPL*KTEIHSTPGRPQ
PTE*RWPRARNTCGSVHPTPLLQQQRCGGPQP*
SDASSTA*PGIPGVQSEAHQPGRSLHPAWPEVH
RLRLGHCHQSPREFS*HSQLCRSKNLSPEEV*GC
LPGADHRWHGLCRQQQRG*HVPVETGIHHVGQA
GLERLTSSDLPPRPPKVPGLQGDSGGPLVCDGA
LQGITSWGSDPCGRSDKPGVYTNICRYLDWIKKI
IGSKG*F*DKH*ISLNKLTTLWFLACLCFGPVG
RAGWGSGIVPAGRLGGDVQKPSSHGHFGHH
Frame 1
SP*FRSPSSQLPVPSDPDIEDSPLPWNVGIQSPSLLLPPEKPRTAPDTLGCLPSAQIQNWELRLLL
PVYRPRPLHFPDLTMGRPRPRAAKTWMFLLLLGG
AWAGHSRAQEDKVLGGHECQPHSQPWQAALFQG
QQLLCGGVLVGGNWVLTAAHCKKPKYTVRLGDH
SLQNKDGPEQEIPVVQSIPHPCYNSSDVEDHNHD
LMLLQLRDQASLGSKVKPISLADHCTQPGQKCT
VSGWGTVTSPRENFPDTLNCAEVKIFPQKKCED
AYPGQITDGMVCAGSSKGADTCQ*RQGFTMLARL
VWNA*LQVIYRLGLPKCRDCRAILEAPWCVMVH
SRASHPGAQTPVGGPTNLASIPTSAATWTGSRR
S*AARADSRISTRSPLINSQLSGSLPVSVLALWG
GLDGDPGLFLLAD*VGMCRNQVLMVTLAI
Frame 2
LPNLGVPAPSSLSPQTQTSRTPPSLGM*ESSPPASSFLQRSPEQPQILSAASPVPKSRTGSSGSSF
LFTGPALSISQTSPWDAPDLVRPRRGCSCSCWG
EPGQDTPGHRRTRCWGVMSANPIRSLGRRPCSRA
SNYSVAVSL*VATGSLQLPTVKNRNTQYAWETT
AYRIKMAQSKKYLWFSPSHTPATTAAMWRTTTM
I*CFFNCVTRHPWGPK*SPSAWQIIAPSLARSAP
SQAGALSPVPERIFLTLSTVQK*KSFPRRSVRM
LTRGRSQMAWSVQAAAKGLTRASRDRDSPCWPG
WSGTPDFK*STASASQSAGIAGRFWRPPGV*WCT
PGHHILGLRPLWEVRQTWRLYQHLPLPGLDQED
HRQQGLILG*ALDLP**THNSLVPCLSLFWPCG
EGWMGIRDCSCWQTRWGCAETKFSWSLWPS
Sequence KLK8.aAug05
GTTCCCAGAAGCTCCCCAGGCTCTAGTGCAGGAGGAGAAGGAGGAGGAGCAGGAGGTGGAGATTCCCAGTTAAAAGGCTCCAGAATCGTGTACCAGGCAGAGAACTGAAGTACTGGGGCCTCCTCCACTGGGTCCGAATCAGTAGGTGACCCCGCCCCTGGATTCTGGAAGACCTCACCATGGGACGCCCCCGACCTCGT
GCGGCCAAGACGTGGATGTTCCTGCTCTTGCTGGGGGGAGCCTGGGCAGCGTGTGGAAGCCTGGACCTCCTCACTAAGTTGTATGCGGAGAACTTGCCGT
GTGTCCATTTGAACCCACAGTGGCCTTCCCAGCCCTCGCACTGCCCCAGAGGGTGGCGATCCAACCCTCTCCCTCCTGCTGCAGGACACTCCAGGGCACA
GGAGGACAAGGTGCTGGGGGGTCATGAGTGCCAACCCCATTCGCAGCCTTGGCAGGCGGCCTTGTTCCAGGGCCAGCAACTACTCTGTGGCGGTGTCCTT
GTAGGTGGCAACTGGGTCCTTACAGCTGCCCACTGTAAAAAACCGAAATACACAGTACGCCTGGGAGACCACAGCCTACAGAATAAAGATGGCCCAGAGC
AAGAAATACCTGTGGTTCAGTCCATCCCACACCCCTGCTACAACAGCAGCGATGTGGAGGACCACAACCATGATCTGATGCTTCTTCAACTGCGTGACCA
GGCATCCCTGGGGTCCAAAGTGAAGCCCATCAGCCTGGCAGATCATTGCACCCAGCCTGGCCAGAAGTGCACCGTCTCAGGCTGGGGCACTGTCACCAGT
CCCCGAGAGAATTTTCCTGACACTCTCAACTGTGCAGAAGTAAAAATCTTTCCCCAGAAGAAGTGTGAGGATGCTTACCCGGGGCAGATCACAGATGGCA
TGGTCTGTGCAGGCAGCAGCAAAGGGGCTGACACGTGCCAGGGCGATTCTGGAGGCCCCCTGGTGTGTGATGGTGCACTCCAGGGCATCACATCCTGGGG
CTCAGACCCCTGTGGGAGGTCCGACAAACCTGGCGTCTATACCAACATCTGCCGCTACCTGGACTGGATCAAGAAGATCATAGGCAGCAAGGGCTGATTC
TAGGATAAGCACTAGATCTCCCTTAATAAACTCACAACTCTCTGGTTCCTTGCCTGTCTCTGTTTTGGCCCTGTGGGGAGGGCTGGATGGGGATCCGGGA
TTGTTCCTGCTGGCAGACTAGGTGGGGATGTGCAGAAACCAAGTTCTCATGGTCACTTTGGCCATCAC
- KLK8.F1 (forward primer)
- KLK8.R13 (reverse primer)
- Alternative acceptor
Frame 0
VPRSSPGSSAGGEGGGAGGGDSQLKGSRIVYQAEN*STGASSTGSESVGDPAPGFWKTSPWDAPDLV
RPRRGCSCSCWGEPGQRVEAWTSSLSCMRRTCR
VSI*THSGLPSPRTAPEGGDPTLSLLLQDTPGH
RRTRCWGVMSANPIRSLGRRPCSRASNYSVAVSL
*VATGSLQLPTVKNRNTQYAWETTAYRIKMAQS
KKYLWFSPSHTPATTAAMWRTTTMI*CFFNCVT
RHPWGPK*SPSAWQIIAPSLARSAPSQAGALSPV
PERIFLTLSTVQK*KSFPRRSVRMLTRGRSQMA
WSVQAAAKGLTRARAILEAPWCVMVHSRASHPG
AQTPVGGPTNLASIPTSAATWTGSRRS*AARADS
RISTRSPLINSQLSGSLPVSVLALWGGLDGDPG
LFLLAD*VGMCRNQVLMVTLAI
Frame 1
FPEAPQALVQEEKEEEQEVEIPS*KAPESCTRQRTEVLGPPPLGPNQ*VTPPLDSGRPHHGTPPTS
CGQDVDVPALAGGSLGSVWKPGPPH*VVCGELAV
CPFEPTVAFPALALPQRVAIQPSPSCCRTLQGT
GGQGAGGS*VPTPFAALAGGLVPGPATTLWRCP
CRWQLGPYSCPL*KTEIHSTPGRPQPTE*RWPRA
RNTCGSVHPTPLLQQQRCGGPQP*SDASSTA*P
GIPGVQSEAHQPGRSLHPAWPEVHRLRLGHCHQ
SPREFS*HSQLCRSKNLSPEEV*GCLPGADHRWH
GLCRQQQRG*HVPGRFWRPPGV*WCTPGHHILG
LRPLWEVRQTWRLYQHLPLPGLDQEDHRQQGLI
LG*ALDLP**THNSLVPCLSLFWPCGEGWMGIRD
CSCWQTRWGCAETKFSWSLWPS
Frame 2
SQKLPRL*CRRRRRRSRRWRFPVKRLQNRVPGRELKYWGLLHWVRISR*PRPWILEDLTMGRPRPR
AAKTWMFLLLLGGAWAACGSLDLLTKLYAENLP
CVHLNPQWPSQPSHCPRGWRSNPLPPAAGHSRAQ
EDKVLGGHECQPHSQPWQAALFQGQQLLCGGVL
VGGNWVLTAAHCKKPKYTVRLGDHSLQNKDGPE
QEIPVVQSIPHPCYNSSDVEDHNHDLMLLQLRDQ
ASLGSKVKPISLADHCTQPGQKCTVSGWGTVTS
PRENFPDTLNCAEVKIFPQKKCEDAYPGQITDG
MVCAGSSKGADTCQGDSGGPLVCDGALQGITSWG
SDPCGRSDKPGVYTNICRYLDWIKKIIGSKG*F
*DKH*ISLNKLTTLWFLACLCFGPVGRAGWGSG
IVPAGRLGGDVQKPSSHGHFGHH
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