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Sequences

Sequence ITGB1.dAug05

ATGAATTTACAACCAATTTTCTGGATTGGACTGATCAGTTCAGTTTGCTGTGTGTTTGCTCAAACAGATGAAAATAGATGTTTAAAAGCAAATGCCAAAT CATGTGGAGAATGTATACAAGCAGGGCCAAATTGTGGGTGGTGCACAAATTCAACATTTTTACAGGAAGGAATGCCTACTTCTGCACGATGTGATGATTT AGAAGCCTTAAAAAAGAAGGGTTGCCCTCCAGATGACATAGAAAATCCCAGAGGCTCCAAAGATATAAAGAAAAATAAAAATGTAACCAACCGTAGCAAA GGAACAGCAGAGAAGCTCAAGCCAGAGGATATTACTCAGATCCAACCACAGCAGTTGGTTTTGCGATTAAGATCAGGGGAGCCACAGACATTTACATTAA AATTCAAGAGAGCTGAAGACTATCCCATTGACCTCTACTACCTTATGGACCTGTCTTACTCAATGAAAGACGATTTGGAGAATGTAAAAAGTCTTGGAAC AGATCTGATGAATGAAATGAGGAGGATTACTTCGGACTTCAGAATTGGATTTGGCTCATTTGTGGAAAAGACTGTGATGCCTTACATTAGCACAACACCA GCTAAGCTCAGGAACCCTTGCACAAGTGAACAGAACTGCACCAGCCCATTTAGCTACAAAAATGTGCTCAGTCTTACTAATAAAGGAGAAGTATTTAATG AACTTGTTGGAAAACAGCGCATATCTGGAAATTTGGATTCTCCAGAAGGTGGTTTCGATGCCATCATGCAAGTTGCAGTTTGTGGATCACTGATTGGCTG GAGGAATGTTACACGGCTGCTGGTGTTTTCCACAGATGCCGGGTTTCACTTTGCTGGAGATGGGAAACTTGGTGGCATTGTTTTACCAAATGATGGACAA TGTCACCTGGAAAATAATATGTACACAATGAGCCATTATTATGATTATCCTTCTATTGCTCACCTTGTCCAGAAACTGAGTGAAAATAATATTCAGACAA TTTTTGCAGTTACTGAAGAATTTCAGCCTGTTTACAAGGAGCTGAAAAACTTGATCCCTAAGTCAGCAGTAGGAACATTATCTGCAAATTCTAGCAATGT AATTCAGTTGATCATTGATGCATACAATTCCCTTTCCTCAGAAGTCATTTTGGAAAACGGCAAATTGTCAGAAGGCGTAACAATAAGTTACAAATCTTAC TGCAAGAACGGGGTGAATGGAACAGGGGAAAATGGAAGAAAATGTTCCAATATTTCCATTGGAGATGAGGTTCAATTTGAAATTAGCATAACTTCAAATA AGTGTCCAAAAAAGGATTCTGACAGCTTTAAAATTAGGCCTCTGGGCTTTACGGAGGAAGTAGAGGTTATTCTTCAGTACATCTGTGAATGTGAATGCCA AAGCGAAGGCATCCCTGAAAGTCCCAAGTGTCATGAAGGAAATGGGACATTTGAGTGTGGCGCGTGCAGGTGCAATGAAGGGCGTGTTGGTAGACATTGT GAATGCAGCACAGATGAAGTTAACAGTGAAGACATGGATGCTTACTGCAGGAAAGAAAACAGTTCAGAAATCTGCAGTAACAATGGAGAGTGCGTCTGCG GACAGTGTGTTTGTAGGAAGAGGGATAATACAAATGAAATTTATTCTGGCAAATTCTGCGAGTGTGATAATTTCAACTGTGATAGATCCAATGGCTTAAT TTGTGGAGGAAATGGTGTTTGCAAGTGTCGTGTGTGTGAGTGCAACCCCAACTACACTGGCAGTGCATGTGACTGTTCTTTGGATACTAGTACTTGTGAA GCCAGCAACGGACAGATCTGCAATGGCCGGGGCATCTGCGAGTGTGGTGTCTGTAAGTGTACAGATCCGAAGTTTCAAGGGCAAACGTGTGAGATGTGTC AGACCTGCCTTGGTGTCTGTGCTGAGCATAAAGAATGTGTTCAGTGCAGAGCCTTCAATAAAGGAGAAAAGAAAGACACATGCACACAGGAATGTTCCTA TTTTAACATTACCAAGGTAGAAAGTCGGGACAAATTACCCCAGCCGGTCCAACCTGATCCTGTGTCCCATTGTAAGGAGAAGGATGTTGACGACTGTTGG TTCTATTTTACGTATTCAGTGAATGGGAACAACGAGGTCATGGTTCATGTTGTGGAGAATCCAGAGTGTCCCACTGGTCCAGACATCATTCCAATTGTAG CTGGTGTGGTTGCTGGAATTGTTCTTATTGGCCTTGCATTACTGCTGATATGGAAGCTTTTAATGATAATTCATGACAGAAGGGAGTTTGCTAAATTTGA AAAGGAGAAAATGAATGCCAAATGGGACACGCCTGGAGTGCAGTGGTGTGATATCAGCTCACTGCAACCTCTGACTTCCAGATTCCAGCAATTCTCCTGC CTCAGCCTCCCGAGTACCTGGGATTACAGGGTGAAAATCCTATTTATAAGAGTGCCGTAACAACTGTGGTCAATCCGAAGTATGAGGGAAAATGAGTACT GCCCGTGCAAATCCCACAACACTGAATGCAAAGTAGCAATTTCCATAGTCACAGTTAGGTAGCTTTAGGGCAATATTGCCATGGTTTTACTCATGTGCAG GTTTTGAAAATGTACAATATGTATAATTTTTAAAATGTTTTATTATTTTGAAAATAATGTTGTAATTCATGCCAGGGACTGACAAAAGACTTGAGACAGG ATGGTTACTCTTGTCAGCTAAGGTCACATTGTGCCTTTTTGACCTTTTCTTCCTGGACTATTGAAATCAAGCTTATTGGATTAAGTGATATTTCTATAGC GATTGAAAGGGCAATAGTTAAAGTAATGAGCATGATGAGAGTTTCTGTTAATCATGTATTAAAACTGATTTTTAGCTTTACAAATATGTCAGTTTGCAGT TATGCAGAATCCAAAGTAAATGTCCTGCTAGCTAGTTAAGGATTGTTTTAAATCTGTTATTTTGCTATTTGCCTGTTAGACATGACTGATGACATATCTG AAAGACAAGTATGTTGAGAGTTGCTGGTGTAAAATACGTTTGAAATAGTTGATCTACAAAGGCCATGGGAAAAATTCAGAGAGTTAGGAAGGAAAAACCA ATAGCTTTAAAACCTGTGTGCCATTTTAAGAGTTACTTAATGTTTGGTAACTTTTATGCCTTCACTTTACAAATTCAAGCCTTAGATAAAAGAACCGAGC AATTTTCTGCTAGCTGAATGGGGACCTTTTGAGTTGAATTTATTTTATTATTTTTATTTTGTTTAATGTCTGGTGCTTTCTGTCACCTCTTCTAATCTTT TAATGTATTTGTTTGCAATTTTGGGGTAAGACTTTTTTTATGAGTACTTTTTCTTTGAAGTTTTAGCGGTCAATTTGCCTTTTTAATGAACATGTGAAGT TATACTGTGGCTATGCAACAGCTCTCACCTACGCGAGTCTTACTTTGAGTTAGTGCCATAACAGACCACTGTATGTTTACTTCTCACCATTTGAGTTGCC CATCTTGTTTCACACTAGTCACATTCTTGTTTTAAGTGCCTTTAGTTTTAACAGTTCACTTTTTACAGTGCTATTTACTGAAGTTATTTATTAAATATGC CTAAAATACTTAAATCGGATGTCTTGACTCTGATGTATTTTATCAGGTTGTGTGCATGAAATTTTTATAGATTAAAGAAGTTGAGGAAAAGCA

ITGB1.F2 (forward primer)
ITGB1.R15 (reverse primer)
Intron retention

Frame 0

MNLQPIFWIGLISSVCCVFAQTDENRCLKANAK SCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDL EALKKKGCPPDDIENPRGSKDIKKNKNVTNRSK GTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTL KFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGT DLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP AKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFN ELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGW RNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQ CHLENNMYTMSHYYDYPSIAHLVQKLSENNIQT IFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNV IQLIIDAYNSLSSEVILENGKLSEGVTISYKSY CKNGVNGTGENGRKCSNISIGDEVQFEISITSN KCPKKDSDSFKIRPLGFTEEVEVILQYICECECQ SEGIPESPKCHEGNGTFECGACRCNEGRVGRHC ECSTDEVNSEDMDAYCRKENSSEICSNNGECVC GQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLI CGGNGVCKCRVCECNPNYTGSACDCSLDTSTCE ASNGQICNGRGICECGVCKCTDPKFQGQTCEMC QTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSY FNITKVESRDKLPQPVQPDPVSHCKEKDVDDCW FYFTYSVNGNNEVMVHVVENPECPTGPDIIPIV AGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFE KEKMNAKWDTPGVQWCDISSLQPLTSRFQQFSC LSLPSTWDYRVKILFIRVP*QLWSIRSMRENEY CPCKSHNTECKVAISIVTVR*L*GNIAMVLLMCR F*KCTICIIFKMFYYFENNVVIHARD*QKT*DR MVTLVS*GHIVPF*PFLPGLLKSSLLD*VIFL* RLKGQ*LK**A**EFLLIMY*N*FLALQICQFAV MQNPK*MSC*LVKDCFKSVILLFAC*T*LMTYL KDKYVESCWCKIRLK*LIYKGHGKNSES*EGKT NSFKTCVPF*ELLNVW*LLCLHFTNSSLR*KNRA IFC*LNGDLLS*IYFIIFILFNVWCFLSPLLIF *CICLQFWGKTFFMSTFSLKF*RSICLFNEHVK LYCGYATALTYASLTLS*CHNRPLYVYFSPFELP ILFHTSHILVLSAFSFNSSLFTVLFTEVIY*IC LKYLNRMS*L*CILSGCVHEIFID*RS*GKA

Frame 1

*IYNQFSGLD*SVQFAVCLLKQMKIDV*KQMPN HVENVYKQGQIVGGAQIQHFYRKECLLLHDVMI *KP*KRRVALQMT*KIPEAPKI*RKIKM*PTVAK EQQRSSSQRILLRSNHSSWFCD*DQGSHRHLH* NSRELKTIPLTSTTLWTCLTQ*KTIWRM*KVLE QI**MK*GGLLRTSELDLAHLWKRL*CLTLAQHQ LSSGTLAQVNRTAPAHLATKMCSVLLIKEKYLM NLLENSAYLEIWILQKVVSMPSCKLQFVDH*LA GGMLHGCWCFPQMPGFTLLEMGNLVALFYQMMDN VTWKIICTQ*AIIMIILLLLTLSRN*VKIIFRQ FLQLLKNFSLFTRS*KT*SLSQQ*EHYLQILAM *FS*SLMHTIPFPQKSFWKTANCQKA*Q*VTNLT ARTG*MEQGKMEENVPIFPLEMRFNLKLA*LQI SVQKRILTALKLGLWALRRK*RLFFSTSVNVNA KAKASLKVPSVMKEMGHLSVARAGAMKGVLVDIV NAAQMKLTVKTWMLTAGKKTVQKSAVTMESASA DSVFVGRGIIQMKFILANSASVIISTVIDPMA* FVEEMVFASVVCVSATPTTLAVHVTVLWILVLVK PATDRSAMAGASASVVSVSVQIRSFKGKRVRCV RPALVSVLSIKNVFSAEPSIKEKRKTHAHRNVP ILTLPR*KVGTNYPSRSNLILCPIVRRRMLTTVG SILRIQ*MGTTRSWFMLWRIQSVPLVQTSFQL* LVWLLELFLLALHYC*YGSF***FMTEGSLLNL KRRK*MPNGTRLECSGVISAHCNL*LPDSSNSPA SASRVPGITG*KSYL*ECRNNCGQSEV*GKMST ARANPTTLNAK*QFP*SQLGSFRAILPWFYSCA GFENVQYV*FLKCFIILKIML*FMPGTDKRLETG WLLLSAKVTLCLFDLFFLDY*NQAYWIK*YFYS D*KGNS*SNEHDESFC*SCIKTDF*LYKYVSLQ LCRIQSKCPAS*LRIVLNLLFCYLPVRHD**HI* KTSMLRVAGVKYV*NS*STKAMGKIQRVRKEKP IALKPVCHFKSYLMFGNFYAFTLQIQALDKRTE QFSAS*MGTF*VEFILLFLFCLMSGAFCHLF*SF NVFVCNFGVRLFL*VLFL*SFSGQFAFLMNM*S YTVAMQQLSPTRVLL*VSAITDHCMFTSHHLSC PSCFTLVTFLF*VPLVLTVHFLQCYLLKLFIKYA *NT*IGCLDSDVFYQVVCMKFL*IKEVEEK

Frame 2

EFTTNFLDWTDQFSLLCVCSNR*K*MFKSKCQI MWRMYTSRAKLWVVHKFNIFTGRNAYFCTM**F RSLKKEGLPSR*HRKSQRLQRYKEK*KCNQP*Q RNSREAQARGYYSDPTTAVGFAIKIRGATDIYIK IQES*RLSH*PLLPYGPVLLNERRFGECKKSWN RSDE*NEEDYFGLQNWIWLICGKDCDALH*HNT S*AQEPLHK*TELHQPI*LQKCAQSY**RRSI** TCWKTAHIWKFGFSRRWFRCHHASCSLWITDWL EECYTAAGVFHRCRVSLCWRWETWWHCFTK*WT MSPGK*YVHNEPLL*LSFYCSPCPETE*K*YSDN FCSY*RISACLQGAEKLDP*VSSRNIICKF*QC NSVDH*CIQFPFLRSHFGKRQIVRRRNNKLQIL LQERGEWNRGKWKKMFQYFHWR*GSI*N*HNFK* VSKKGF*QL*N*ASGLYGGSRGYSSVHL*M*MP KRRHP*KSQVS*RKWDI*VWRVQVQ*RACW*TL *MQHR*S*Q*RHGCLLQERKQFRNLQ*QWRVRLR TVCL*EEG*YK*NLFWQILRV**FQL**IQWLN LWRKWCLQVSCV*VQPQLHWQCM*LFFGY*YL* SQQRTDLQWPGHLRVWCL*VYRSEVSRANV*DVS DLPWCLC*A*RMCSVQSLQ*RRKERHMHTGMFL F*HYQGRKSGQITPAGPT*SCVPL*GEGC*RLL VLFYVFSEWEQRGHGSCCGESRVSHWSRHHSNCS WCGCWNCSYWPCITADMEAFNDNS*QKGVC*I* KGENECQMGHAWSAVV*YQLTATSDFQIPAILL PQPPEYLGLQGENPIYKSAVTTVVNPKYEGK*VL PVQIPQH*MQSSNFHSHS*VALGQYCHGFTHVQ VLKMYNMYNF*NVLLF*K*CCNSCQGLTKDLRQ DGYSCQLRSHCAFLTFSSWTIEIKLIGLSDISIA IERAIVKVMSMMRVSVNHVLKLIFSFTNMSVCS YAESKVNVLLAS*GLF*ICYFAICLLDMTDDIS ERQVC*ELLV*NTFEIVDLQRPWEKFRELGRKNQ *L*NLCAILRVT*CLVTFMPSLYKFKP*IKEPS NFLLAEWGPFELNLFYYFYFV*CLVLSVTSSNL LMYLFAILG*DFFYEYFFFEVLAVNLPF**TCEV ILWLCNSSHLRESYFELVP*QTTVCLLLTI*VA HLVSH*SHSCFKCL*F*QFTFYSAIY*SYLLNM PKILKSDVLTLMYFIRLCA*NFYRLKKLRKS

Sequence ITGB1.fAug05

ATGAATTTACAACCAATTTTCTGGATTGGACTGATCAGTTCAGTTTGCTGTGTGTTTGCTCAAACAGATGAAAATAGATGTTTAAAAGCAAATGCCAAAT CATGTGGAGAATGTATACAAGCAGGGCCAAATTGTGGGTGGTGCACAAATTCAACATTTTTACAGGAAGGAATGCCTACTTCTGCACGATGTGATGATTT AGAAGCCTTAAAAAAGAAGGGTTGCCCTCCAGATGACATAGAAAATCCCAGAGGCTCCAAAGATATAAAGAAAAATAAAAATGTAACCAACCGTAGCAAA GGAACAGCAGAGAAGCTCAAGCCAGAGGATATTACTCAGATCCAACCACAGCAGTTGGTTTTGCGATTAAGATCAGGGGAGCCACAGACATTTACATTAA AATTCAAGAGAGCTGAAGACTATCCCATTGACCTCTACTACCTTATGGACCTGTCTTACTCAATGAAAGACGATTTGGAGAATGTAAAAAGTCTTGGAAC AGATCTGATGAATGAAATGAGGAGGATTACTTCGGACTTCAGAATTGGATTTGGCTCATTTGTGGAAAAGACTGTGATGCCTTACATTAGCACAACACCA GCTAAGCTCAGGAACCCTTGCACAAGTGAACAGAACTGCACCAGCCCATTTAGCTACAAAAATGTGCTCAGTCTTACTAATAAAGGAGAAGTATTTAATG AACTTGTTGGAAAACAGCGCATATCTGGAAATTTGGATTCTCCAGAAGGTGGTTTCGATGCCATCATGCAAGTTGCAGTTTGTGGATCACTGATTGGCTG GAGGAATGTTACACGGCTGCTGGTGTTTTCCACAGATGCCGGGTTTCACTTTGCTGGAGATGGGAAACTTGGTGGCATTGTTTTACCAAATGATGGACAA TGTCACCTGGAAAATAATATGTACACAATGAGCCATTATTATGATTATCCTTCTATTGCTCACCTTGTCCAGAAACTGAGTGAAAATAATATTCAGACAA TTTTTGCAGTTACTGAAGAATTTCAGCCTGTTTACAAGGAGCTGAAAAACTTGATCCCTAAGTCAGCAGTAGGAACATTATCTGCAAATTCTAGCAATGT AATTCAGTTGATCATTGATGCATACAATTCCCTTTCCTCAGAAGTCATTTTGGAAAACGGCAAATTGTCAGAAGGCGTAACAATAAGTTACAAATCTTAC TGCAAGAACGGGGTGAATGGAACAGGGGAAAATGGAAGAAAATGTTCCAATATTTCCATTGGAGATGAGGTTCAATTTGAAATTAGCATAACTTCAAATA AGTGTCCAAAAAAGGATTCTGACAGCTTTAAAATTAGGCCTCTGGGCTTTACGGAGGAAGTAGAGGTTATTCTTCAGTACATCTGTGAATGTGAATGCCA AAGCGAAGGCATCCCTGAAAGTCCCAAGTGTCATGAAGGAAATGGGACATTTGAGTGTGGCGCGTGCAGGTGCAATGAAGGGCGTGTTGGTAGACATTGT GAATGCAGCACAGATGAAGTTAACAGTGAAGACATGGATGCTTACTGCAGGAAAGAAAACAGTTCAGAAATCTGCAGTAACAATGGAGAGTGCGTCTGCG GACAGTGTGTTTGTAGGAAGAGGGATAATACAAATGAAATTTATTCTGGCAAATTCTGCGAGTGTGATAATTTCAACTGTGATAGATCCAATGGCTTAAT TTGTGGAGGAAATGGTGTTTGCAAGTGTCGTGTGTGTGAGTGCAACCCCAACTACACTGGCAGTGCATGTGACTGTTCTTTGGATACTAGTACTTGTGAA GCCAGCAACGGACAGATCTGCAATGGCCGGGGCATCTGCGAGTGTGGTGTCTGTAAGTGTACAGATCCGAAGTTTCAAGGGCAAACGTGTGAGATGTGTC AGACCTGCCTTGGTGTCTGTGCTGAGCATAAAGAATGTGTTCAGTGCAGAGCCTTCAATAAAGGAGAAAAGAAAGACACATGCACACAGGAATGTTCCTA TTTTAACATTACCAAGGTAGAAAGTCGGGACAAATTACCCCAGCCGGTCCAACCTGATCCTGTGTCCCATTGTAAGGAGAAGGATGTTGACGACTGTTGG TTCTATTTTACGTATTCAGTGAATGGGAACAACGAGGTCATGGTTCATGTTGTGGAGAATCCAGAGTGTCCCACTGGTCCAGACATCATTCCAATTGTAG CTGGTGTGGTTGCTGGAATTGTTCTTATTGGCCTTGCATTACTGCTGATATGGAAGCTTTTAATGATAATTCATGACAGAAGGGAGTTTGCTAAATTTGA AAAGGAGAAAATGAATGCCAAATGGGACACGGTAAGTTACAAAACATCCAAAAAGCAAAGTGGCTTATAAAGTAAATGTAATACTCCTAAGACTTATGTA TTAGCTGTCAGGCTGATTATTAAAGTCCTTTCTAAGTATTTTATTCCCCCAAAAGTTTCTTACTCAAGGAATTTGCATTTAGTGAAAAACAGAAAGCATC CTAAATATATCCCATTGAAACAAAACATTGATTATAAGCATGTATATTCTGGTTACTGTGGCCAGTATTTTTATTTCTTTAATAGTTTTGATCCTAAATC TGCCTTTTCATCTAATGTGAAGTAGAATCCTAAATAATGTTATCTGTGTAGCAAGCTATTCAATGGAAAGCTGCTTCTTTCTTTAAAACAAACAAACAAA AAAAACCTTCAGTGAAAGCCAGATTCAAAAGGTTATATACCAAGCTTGTCCAACTCGCAGCTCGTCGGCCAGGACATGCAGCCCAGAATAGCTTTGAATG TGGCCCCAACACAAATTTGTAAACTTTCTTAGAAATTGTAATTATTATTATTATTTTTTTTTGGTAACTTTTTTTTAAAGCTCATCAGCTATCGTTAGTG TATTTTATGTGTGGCCCAAGACAATTCTTCTTCTTCCAGTGTGGCCCAGGGAAGCCAAAAGATTGGACACCCCTGCTATATACTATATGATTCCATTTAG AGGACATTCTGGAAAAGCAAAACTGTAGGGGCAAAAATCAGTGGTTGCTAGGGGCTGGAATGGGGGAAAGTGTTGACCACAGAGGGGCGTAAGGGATCTT CCTTGGGATGATCTTGATTGTGGGTGGATTTATGTATTTGAAAACTCACAGAACTATGTACTTTAAAAAGATGTATGTTCCTCTATGAAAATTATATCTC AGTAAACTTTGGCTTATAAAAATCTTAAAAGCCCTAAGTGACCGAAAGGTTATGTTAGCATTGAGTGCTTTGAAATATGGAGTCAGAGGGTGGGGTAACC AAATGTTGGCCTTTGTGTATTCATCTTTTGATACAAGAAAGCAATGCCAATCTTCAGTATTTTTAAATTGTAAATGAATTTTGTAGTTAGTTGCACTTTA CTACAATTTGCTTGGGTTTTTAAAAATTATCAATTTTTTAAATTACAAATTTCAGAAGGGTAGAAATAAAAAAAAAGACATTTATAGTGTTCAAACACTA TAAAGGGATTTTTTTTTTCTGATGAAATCATTAGAAACTATTTCTTTTACTTTAGCAAGAAAATCCGATTTACAAGAGTCCTATTAATAATTTCAAGAAT CCAAACTACGGACGTAAAGCTGGTCTCTAAATTGCCGTTCGTATTTTGTTTCACTTTCTGCCTTTGCATGTGAACTTCTATCTTTTTTACTGCACTGTGT GTCCTTTATCACTATTGCTGTTCTTTTTTTAAGTCTGGCTTTAAATATCGCTATTGTGGCTTTTCTTTTTGTATGTTAACTTCGCGTTTAGAAAGACCGT AAAATAAGTAATGTGATATAGAAACAGCAGCCATGTTGTAGTGAAAAGAGAATGTGCTTTGCAATCAGTTAGACCTCAGGTCAAATCCCACCTCTTCCAC TTAGTAAGTAGAACAGCATTGAGTCACTTCACTTCTCTCAACTGTCACAGGGAGTCAGTACTCTGTTTTGTATGGGGTGGTTGTATTTGGCAAATGCACT CCAGTATATGAAATCAGCCTGTCAGCTCTAATCCTCATAACAGTCCTGTGCCTAGAATGATATTATCCTATTATCTTTTGTTCTTTTTCTGTTTATTTAT TTATTTATTTTGAGACAGTCTCTCTCTGTCGCCCAGCCTGGAGTGCAGTGGTGTGATATCAGCTCACTGCAACCTCTGACTTCCAGATTCCAGCAATTCT CCTGCCTCAGCCTCCCGAGTACCTGGGATTACAGGTGCCTGCCACCATGCTAGGCTAATTTTTTTGTTTTTAGTAGAGATGGGGTTTCACCACATTGGCT GGGCTGGCCTTGA

ITGB1.F2 (forward primer)
ITGB1.R15 (reverse primer)
Intron retention

Frame 0

MNLQPIFWIGLISSVCCVFAQTDENRCLKANAK SCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDL EALKKKGCPPDDIENPRGSKDIKKNKNVTNRSK GTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTL KFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGT DLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP AKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFN ELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGW RNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQ CHLENNMYTMSHYYDYPSIAHLVQKLSENNIQT IFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNV IQLIIDAYNSLSSEVILENGKLSEGVTISYKSY CKNGVNGTGENGRKCSNISIGDEVQFEISITSN KCPKKDSDSFKIRPLGFTEEVEVILQYICECECQ SEGIPESPKCHEGNGTFECGACRCNEGRVGRHC ECSTDEVNSEDMDAYCRKENSSEICSNNGECVC GQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLI CGGNGVCKCRVCECNPNYTGSACDCSLDTSTCE ASNGQICNGRGICECGVCKCTDPKFQGQTCEMC QTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSY FNITKVESRDKLPQPVQPDPVSHCKEKDVDDCW FYFTYSVNGNNEVMVHVVENPECPTGPDIIPIV AGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFE KEKMNAKWDTVSYKTSKKQSGL*SKCNTPKTYV LAVRLIIKVLSKYFIPPKVSYSRNLHLVKNRKH PKYIPLKQNIDYKHVYSGYCGQYFYFFNSFDPKS AFSSNVK*NPK*CYLCSKLFNGKLLLSLKQTNK KNLQ*KPDSKGYIPSLSNSQLVGQDMQPRIALN VAPTQICKLS*KL*LLLLFFFGNFFLKLISYR*C ILCVAQDNSSSSSVAQGSQKIGHPCYILYDSI* RTFWKSKTVGAKISGC*GLEWGKVLTTEGRKGS SLG*S*LWVDLCI*KLTELCTLKRCMFLYENYIS VNFGL*KS*KP*VTERLC*H*VL*NMESEGGVT KCWPLCIHLLIQESNANLQYF*IVNEFCS*LHF TTICLGF*KLSIF*ITNFRRVEIKKKTFIVFKHY KGIFFF**NH*KLFLLL*QENPIYKSPINNFKN PNYGRKAGL*IAVRILFHFLPLHVNFYLFYCTV CPLSLLLFFF*VWL*ISLLWLFFLYVNFAFRKTV K*VM*YRNSSHVVVKRECALQSVRPQVKSHLFH LVSRTALSHFTSLNCHRESVLCFVWGGCIWQMH SSI*NQPVSSNPHNSPVPRMILSYYLLFFFCLFI YLF*DSLSLSPSLECSGVISAHCNL*LPDSSNS PASASRVPGITGACHHARLIFLFLVEMGFHHIG WAGL

Frame 1

*IYNQFSGLD*SVQFAVCLLKQMKIDV*KQMPN HVENVYKQGQIVGGAQIQHFYRKECLLLHDVMI *KP*KRRVALQMT*KIPEAPKI*RKIKM*PTVAK EQQRSSSQRILLRSNHSSWFCD*DQGSHRHLH* NSRELKTIPLTSTTLWTCLTQ*KTIWRM*KVLE QI**MK*GGLLRTSELDLAHLWKRL*CLTLAQHQ LSSGTLAQVNRTAPAHLATKMCSVLLIKEKYLM NLLENSAYLEIWILQKVVSMPSCKLQFVDH*LA GGMLHGCWCFPQMPGFTLLEMGNLVALFYQMMDN VTWKIICTQ*AIIMIILLLLTLSRN*VKIIFRQ FLQLLKNFSLFTRS*KT*SLSQQ*EHYLQILAM *FS*SLMHTIPFPQKSFWKTANCQKA*Q*VTNLT ARTG*MEQGKMEENVPIFPLEMRFNLKLA*LQI SVQKRILTALKLGLWALRRK*RLFFSTSVNVNA KAKASLKVPSVMKEMGHLSVARAGAMKGVLVDIV NAAQMKLTVKTWMLTAGKKTVQKSAVTMESASA DSVFVGRGIIQMKFILANSASVIISTVIDPMA* FVEEMVFASVVCVSATPTTLAVHVTVLWILVLVK PATDRSAMAGASASVVSVSVQIRSFKGKRVRCV RPALVSVLSIKNVFSAEPSIKEKRKTHAHRNVP ILTLPR*KVGTNYPSRSNLILCPIVRRRMLTTVG SILRIQ*MGTTRSWFMLWRIQSVPLVQTSFQL* LVWLLELFLLALHYC*YGSF***FMTEGSLLNL KRRK*MPNGTR*VTKHPKSKVAYKVNVILLRLMY *LSG*LLKSFLSILFPQKFLTQGICI**KTESI LNISH*NKTLIISMYILVTVASIFISLIVLILN LPFHLM*SRILNNVICVASYSMESCFFL*NKQTK KTFSESQIQKVIYQACPTRSSSARTCSPE*L*M WPQHKFVNFLRNCNYYYYFFLVTFF*SSSAIVS VFYVWPKTILLLPVWPREAKRLDTPAIYYMIPFR GHSGKAKL*GQKSVVARGWNGGKC*PQRGVRDL PWDDLDCGWIYVFENSQNYVL*KDVCSSMKIIS Q*TLAYKNLKSPK*PKGYVSIECFEIWSQRVG*P NVGLCVFIF*YKKAMPIFSIFKL*MNFVVSCTL LQFAWVFKNYQFFKLQISEG*K*KKRHL*CSNT IKGFFFSDEIIRNYFFYFSKKIRFTRVLLIISRI QTTDVKLVSKLPFVFCFTFCLCM*TSIFFTALC VLYHYCCSFFKSGFKYRYCGFSFCMLTSRLERP *NK*CDIETAAML**KENVLCNQLDLRSNPTSST **VEQH*VTSLLSTVTGSQYSVLYGVVVFGKCT PVYEISLSALILITVLCLE*YYPIIFCSFSVYL FIYFETVSLCRPAWSAVV*YQLTATSDFQIPAIL LPQPPEYLGLQVPATMLG*FFCF**RWGFTTLA GLAL

Frame 2

EFTTNFLDWTDQFSLLCVCSNR*K*MFKSKCQI MWRMYTSRAKLWVVHKFNIFTGRNAYFCTM**F RSLKKEGLPSR*HRKSQRLQRYKEK*KCNQP*Q RNSREAQARGYYSDPTTAVGFAIKIRGATDIYIK IQES*RLSH*PLLPYGPVLLNERRFGECKKSWN RSDE*NEEDYFGLQNWIWLICGKDCDALH*HNT S*AQEPLHK*TELHQPI*LQKCAQSY**RRSI** TCWKTAHIWKFGFSRRWFRCHHASCSLWITDWL EECYTAAGVFHRCRVSLCWRWETWWHCFTK*WT MSPGK*YVHNEPLL*LSFYCSPCPETE*K*YSDN FCSY*RISACLQGAEKLDP*VSSRNIICKF*QC NSVDH*CIQFPFLRSHFGKRQIVRRRNNKLQIL LQERGEWNRGKWKKMFQYFHWR*GSI*N*HNFK* VSKKGF*QL*N*ASGLYGGSRGYSSVHL*M*MP KRRHP*KSQVS*RKWDI*VWRVQVQ*RACW*TL *MQHR*S*Q*RHGCLLQERKQFRNLQ*QWRVRLR TVCL*EEG*YK*NLFWQILRV**FQL**IQWLN LWRKWCLQVSCV*VQPQLHWQCM*LFFGY*YL* SQQRTDLQWPGHLRVWCL*VYRSEVSRANV*DVS DLPWCLC*A*RMCSVQSLQ*RRKERHMHTGMFL F*HYQGRKSGQITPAGPT*SCVPL*GEGC*RLL VLFYVFSEWEQRGHGSCCGESRVSHWSRHHSNCS WCGCWNCSYWPCITADMEAFNDNS*QKGVC*I* KGENECQMGHGKLQNIQKAKWLIK*M*YS*DLC ISCQADY*SPF*VFYSPKSFLLKEFAFSEKQKAS *IYPIETKH*L*ACIFWLLWPVFLFL**F*S*I CLFI*CEVES*IMLSV*QAIQWKAASFFKTNKQ KKPSVKARFKRLYTKLVQLAARRPGHAAQNSFEC GPNTNL*TFLEIVIIIIIFFW*LFFKAHQLSLV YFMCGPRQFFFFQCGPGKPKDWTPLLYTI*FHL EDILEKQNCRGKNQWLLGAGMGESVDHRGA*GIF LGMILIVGGFMYLKTHRTMYFKKMYVPL*KLYL SKLWLIKILKALSDRKVMLALSALKYGVRGWGN QMLAFVYSSFDTRKQCQSSVFLNCK*IL*LVALY YNLLGFLKIINFLNYKFQKGRNKKKDIYSVQTL *RDFFFLMKSLETISFTLARKSDLQESY**FQE SKLRT*SWSLNCRSYFVSLSAFACELLSFLLHCV SFITIAVLFLSLALNIAIVAFLFVC*LRV*KDR KISNVI*KQQPCCSEKRMCFAIS*TSGQIPPLP LSK*NSIESLHFSQLSQGVSTLFCMGWLYLANAL QYMKSACQL*SS*QSCA*NDIILLSFVLFLFIY LFILRQSLSVAQPGVQWCDISSLQPLTSRFQQF SCLSLPSTWDYRCLPPC*ANFFVFSRDGVSPHWL GWP*