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Sequences

Sequence FGFR2.pAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGGCCGCCGG TGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGG ATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTT ACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAA CGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGC TTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTC CCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTC AAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGC TGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACC TAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTG CTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCT ATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGG AAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAG GTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTA ATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAG CCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACA CAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATA CCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAA TGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTA ATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTARPP VLTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLG YPFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRNTWTSANLSNSIHLV TLTQEVLVLQEMILFFLQTPCLTNHAFLSIHT* TAVLKHE*LCLPVPKQDSTGNLATLSRETMPPRA CCLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIF PRRRRRFLEQWTATSHHVTPLTCRAYWLWTSRT QGGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGA GCM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT *KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTF AFRENGITLIY**IGNIQNN*SFIVFFVI*VAFL CRQHSRLVNLLLGLN*LSDPLKREYLQYMTNLG KMKF*FICV*MLLSDDCS*TS*MPHIKRTHS*E GVSFWCATLSLRQRNV*LDFPR*MVPASS*KMP* SIP*GQTLVEMIAECASLWQLAFCF*VAH*SD* PVFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*N TELFC**FWGYVHLFKGLLSSNSGRTSPKDPASY LHQTKYRRCSFCTKVLCFALETPTHFAIAVQDE CRLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDI NVNKTK*MSRNLFF*

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQGRR C*HHGQRD*GSLYSECNF*GRWGIYVLGG*FYW DILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*L P*HKKFLFFRR*FCFFSRPHALRTMPSSVSTHK RQC*NMNDCVCLSPNRTALGT*LH*AGRPCLPE LVVSTCIYGSEE*IIGKVISICVKIYTVENL*SS PGGEEGFWSSGLPQATM*PLSPAVRTGCGPVGL KVDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*V LDVCKYIQIMYKYILYIYKELFFVLILNGCPNAP RKLVSLFLIAIC*MLFLHIIS*FSPSRGGKILL LSGKMV*R*FINKLVIYKTINHL*FFL*FKWHF YAGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWG K*SFDLFVFKCCCQTIVLRPPKCPILKELIHRK VFHFGVQPCHYVNATSNWTSQDKWYQRPLKRCL NPFLEDRP*LK**QNVLLSGSWPSASELHINQIS LYSLQ*ILIMASRLFGVGDAC*DLQVPS*KIET QSCSADSFGDTSIFLRDCFHLILAGPHQKIQPH TYIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMN ADYTDLMCYKIGESI**NLLIFILTIKMFLQIL MLTRQNKCHATYFF

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKAAG VNTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIG ISFHSAWLTVLPAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPS YPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHIN GSVKT*MTVSACPQTGQHWEPSYTEQGDHASQS LLSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNL PQEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DS RWTCVLPSLLIL**LEKIYVSTHLQSTNAVYRC WMYVNIFKLCINIYYIFTRSYFLY*F*MDVPMH LENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFC FQGKWYNVNLLINW*YTKQLIIYSFFCNLSGIS MQAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FG ENEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGR CFILVCNPVITSTQRLTGLPKINGTSVLLKDAL IHSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRL ACILFSEF**WLPDSLALETPVRIFKSHHRKLKH RVVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLI PTSDKISPLFLLY*SIVFCFGNTHSLCNSRAR* MQITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY* C*QDKINVTQLIFL

Sequence FGFR2.lAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGT AACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGA GAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCC GAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTC CAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAA CTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAG TGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGA GATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCC TCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCA AGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGT AACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTC AAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGG GCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACAT GATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAG GAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAG ACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCA CTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAA TCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTA ACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGAT TTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACA AGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACA CATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAA ACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATC AGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCT TAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGG ACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCT CTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGG AGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGC TTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTT TGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAA CCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQGRRC*HHGQRD*GSLYSEC NF*GRWGIYVLGG*FYWDILSLCMVDSSASAWK RKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPVP NEEHDQEARLQQPAGCAQADQTYPPAETGFG*V QLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV* TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGSS GN*QRQAQGGGHRGREDVER*CHRERPF*SGVR DGDDEDDWETQEYHKSSWSLHTGWASLCHS*VC L*RQPPRIPPSPEATRDGVLL*H*PCS*GADDLQ GLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCFG NRKQCDENSRLWTRQRYQQYRLLQKDHQWAASS QVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFRG LALPRDSRGGTF*AAEGRTQNG*ASQLHQRTVH DDEGLLACSALPETNVQAVGRRLGSNSHSHNQ* GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFSR PHALRTMPSSVSTHKRQC*NMNDCVCLSPNRTA LGT*LH*AGRPCLPELVVSTCIYGSEE*IIGKV ISICVKIYTVENL*SSPGGEEGFWSSGLPQATM* PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGED LCQHTLTEHKCSI*VLDVCKYIQIMYKYILYIY KELFFVLILNGCPNAPRKLVSLFLIAIC*MLFLH IIS*FSPSRGGKILLLSGKMV*R*FINKLVIYK TINHL*FFL*FKWHFYAGSTAD*LIYCLDLTSY QIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIVL RPPKCPILKELIHRKVFHFGVQPCHYVNATSNW TSQDKWYQRPLKRCLNPFLEDRP*LK**QNVLL SGSWPSASELHINQISLYSLQ*ILIMASRLFGVG DAC*DLQVPS*KIETQSCSADSFGDTSIFLRDC FHLILAGPHQKIQPHTYIRQNIAVVPSVLKYCV LLWKHPLTLQ*PCKMNADYTDLMCYKIGESI**N LLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKAAGVNTTDKEIEVLYIRNV TFEDAGEYTCLAGNSIGISFHSAWLTVLPAPGR EKEITASPDYLEIAIYCIGVFLIACMVVTVILC RMKNTTKKPDFSSQPAVHKLTKRIPLRRQVSAES SSSMNSNTPLVRITTRLSSTADTPMLAGVSEYE LPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEA VGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSE MEMMKMIGKHKNIINLLGACTQDGPLYVIVEYA SKGNLREYLRARRPPGMEYSYDINRVPEEQMTF KDLVSCTYQLARGMEYLASQKCIHRDLAARNVLV TENNVMKIADFGLARDINNIDYYKKTTNGRLPV KWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLG GSPYPGIPVEELFKLLKEGHRMDKPANCTNELYM MMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNE EYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSP DPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQH WEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK* SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPPC NPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEKI YVSTHLQSTNAVYRCWMYVNIFKLCINIYYIFT RSYFLY*F*MDVPMHLENWSLFF**LFAKCCSY T*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YTK QLIIYSFFCNLSGISMQAAQQTS*SIAWT*LVI RSFEKRIFTIYD*FGENEVLIYLCLNAAVRRLF LDLLNAPY*KNSFIGRCFILVCNPVITSTQRLTG LPKINGTSVLLKDALIHSLRTDLS*NDSRMCFS LAAGLLLLSCTLIRLACILFSEF**WLPDSLAL ETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GIA FI*FWQDLTKRSSLIPTSDKISPLFLLY*SIVF CFGNTHSLCNSRAR*MQITLILCVTKLEKVFNK TC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSRPPVLTPRTKRLRFSIFGM *LLRTLGNIRAWRVILLGYPFTLHG*QFCQRLEE KRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSCA E*RTRPRSQTSAASRLCTS*PNVSPCGDRFRLS PAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSMN FQRTQNGSFQEIS*HWASPWEKVALGKWSWRKQ WELTKTSPRRRSPWP*RC*KMMPQRKTFLIWCQ RWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSMP LKATSENTSEPGGHPGWSTPMTLTVFLRSR*PS RTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMFW *QKTM**K*QTLDSPEISTI*TITKRPPMGGFQS SGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL*G ARPTQGFPWRNFLSC*RKDTEWISQPTAPTNCT ***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPMR NTWTSANLSNSIHLVTLTQEVLVLQEMILFFLQ TPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQDS TGNLATLSRETMPPRACCLHLYIWIRGVNNWKSN QHMCKDLYS*KLVIFPRRRRRFLEQWTATSHHV TPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWRR FMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYLQ GVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVLT HNFLIFTEQRWKNTFAFRENGITLIY**IGNIQ NN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*LS DPLKREYLQYMTNLGKMKF*FICV*MLLSDDCS *TS*MPHIKRTHS*EGVSFWCATLSLRQRNV*L DFPR*MVPASS*KMP*SIP*GQTLVEMIAECASL WQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWRW RRLLGSSSPIIEN*NTELFC**FWGYVHLFKGL LSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLCF ALETPTHFAIAVQDECRLH*SYVLQNWRKYLIK PVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.kAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGA AGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGT GCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGA GTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTAT GAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAG CAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTC AGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTAT GCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCT TCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTT GGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCA GTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAG GGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTA CATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAAT GAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTC CAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACA GCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAG TAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCAT GTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAA GATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTT ACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTT ACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATAC AAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTT ATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGT TCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAAC TGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTC TCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGT TGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGAT TGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTG TTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATA AAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTASAW KRKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPV PNEEHDQEARLQQPAGCAQADQTYPPAETGFG* VQLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV *TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGS SGN*QRQAQGGGHRGREDVER*CHRERPF*SGV RDGDDEDDWETQEYHKSSWSLHTGWASLCHS*V CL*RQPPRIPPSPEATRDGVLL*H*PCS*GADDL QGLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCF GNRKQCDENSRLWTRQRYQQYRLLQKDHQWAAS SQVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFR GLALPRDSRGGTF*AAEGRTQNG*ASQLHQRTV HDDEGLLACSALPETNVQAVGRRLGSNSHSHNQ *GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFS RPHALRTMPSSVSTHKRQC*NMNDCVCLSPNRT ALGT*LH*AGRPCLPELVVSTCIYGSEE*IIGK VISICVKIYTVENL*SSPGGEEGFWSSGLPQATM *PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGE DLCQHTLTEHKCSI*VLDVCKYIQIMYKYILYI YKELFFVLILNGCPNAPRKLVSLFLIAIC*MLFL HIIS*FSPSRGGKILLLSGKMV*R*FINKLVIY KTINHL*FFL*FKWHFYAGSTAD*LIYCLDLTS YQIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIV LRPPKCPILKELIHRKVFHFGVQPCHYVNATSN WTSQDKWYQRPLKRCLNPFLEDRP*LK**QNVL LSGSWPSASELHINQISLYSLQ*ILIMASRLFGV GDAC*DLQVPS*KIETQSCSADSFGDTSIFLRD CFHLILAGPHQKIQPHTYIRQNIAVVPSVLKYC VLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI** NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQAPG REKEITASPDYLEIAIYCIGVFLIACMVVTVIL CRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVSAE SSSSMNSNTPLVRITTRLSSTADTPMLAGVSEY ELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAE AVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVS EMEMMKMIGKHKNIINLLGACTQDGPLYVIVEY ASKGNLREYLRARRPPGMEYSYDINRVPEEQMT FKDLVSCTYQLARGMEYLASQKCIHRDLAARNVL VTENNVMKIADFGLARDINNIDYYKKTTNGRLP VKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTL GGSPYPGIPVEELFKLLKEGHRMDKPANCTNELY MMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTN EEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFS PDPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQ HWEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK *SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPP CNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEK IYVSTHLQSTNAVYRCWMYVNIFKLCINIYYIF TRSYFLY*F*MDVPMHLENWSLFF**LFAKCCS YT*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YT KQLIIYSFFCNLSGISMQAAQQTS*SIAWT*LV IRSFEKRIFTIYD*FGENEVLIYLCLNAAVRRL FLDLLNAPY*KNSFIGRCFILVCNPVITSTQRLT GLPKINGTSVLLKDALIHSLRTDLS*NDSRMCF SLAAGLLLLSCTLIRLACILFSEF**WLPDSLA LETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GI AFI*FWQDLTKRSSLIPTSDKISPLFLLY*SIV FCFGNTHSLCNSRAR*MQITLILCVTKLEKVFN KTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKRLE EKRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSC AE*RTRPRSQTSAASRLCTS*PNVSPCGDRFRL SPAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSM NFQRTQNGSFQEIS*HWASPWEKVALGKWSWRK QWELTKTSPRRRSPWP*RC*KMMPQRKTFLIWC QRWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSM PLKATSENTSEPGGHPGWSTPMTLTVFLRSR*P SRTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMF W*QKTM**K*QTLDSPEISTI*TITKRPPMGGFQ SSGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL* GARPTQGFPWRNFLSC*RKDTEWISQPTAPTNC T***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPM RNTWTSANLSNSIHLVTLTQEVLVLQEMILFFL QTPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQD STGNLATLSRETMPPRACCLHLYIWIRGVNNWKS NQHMCKDLYS*KLVIFPRRRRRFLEQWTATSHH VTPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWR RFMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYL QGVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVL THNFLIFTEQRWKNTFAFRENGITLIY**IGNI QNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*L SDPLKREYLQYMTNLGKMKF*FICV*MLLSDDC S*TS*MPHIKRTHS*EGVSFWCATLSLRQRNV* LDFPR*MVPASS*KMP*SIP*GQTLVEMIAECAS LWQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWR WRRLLGSSSPIIEN*NTELFC**FWGYVHLFKG LLSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLC FALETPTHFAIAVQDECRLH*SYVLQNWRKYLI KPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.iAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGG CTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTA AGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGT AGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAAT GCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCA GTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGA GGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGA AGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGT GCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTC GGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCC GAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGG CGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCT GGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTT GAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGA TGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAA TGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGG CTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCA CTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGA ACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACA ACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTT TTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAAC AGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTG GAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGC CACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATT GGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTAT ATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCT GTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTA ATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAA CTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGAC GATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACG TCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATG TGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTT TGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTA AGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGT ATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTAT TTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRSTILDQHRKDGKA APCCACGQHCQVSLPSRGEPNANHAVAEKREGV* AGASHWRLQGTKPALEPHYGKCGPI*QGKLYLC SGE*IRVHQSHVPPGCCGAIASPAHPPSRTAGK CLHSGRRRRRVCLQGLQ*CPAPHPVDQARGKERQ *IRARRAALPQGSQALGDK*FQCRSAGSVQCDR GGCWGIYM*GLQLYRAGQPVCLAHCPAKTASAW KRKGDYSFPRLPGDSHLLHRGLLNRLYGGNSHPV PNEEHDQEARLQQPAGCAQADQTYPPAETGNSF G*VQLLHELQHPAGEDNNTPLFNGRHPHAGRGL RV*TSRGPKMGVSKR*ADTGQAPGRRLLWASGHG GSSGN*QRQAQGGGHRGREDVER*CHRERPF*S GVRDGDDEDDWETQEYHKSSWSLHTGWASLCHS *VCL*RQPPRIPPSPEATRDGVLL*H*PCS*GAD DLQGLGVMHLPAGQRHGVLGFPKMYSSRFSSQK CFGNRKQCDENSRLWTRQRYQQYRLLQKDHQWA ASSQVDGSRSPV**SIHSSE*CLVLRGVNVGDLH FRGLALPRDSRGGTF*AAEGRTQNG*ASQLHQR TVHDDEGLLACSALPETNVQAVGRRLGSNSHSH NQ*GILGPQPTSRTVFT*LP*HKKFLFFRR*FCF FSRPHALRTMPSSVSTHKRQC*NMNDCVCLSPN RTALGT*LH*AGRPCLPELVVSTCIYGSEE*II GKVISICVKIYTVENL*SSPGGEEGFWSSGLPQA TM*PLSPAVRTGCGPVGLKVDVRSAFLVNFVII GEDLCQHTLTEHKCSI*VLDVCKYIQIMYKYIL YIYKELFFVLILNGCPNAPRKLVSLFLIAIC*ML FLHIIS*FSPSRGGKILLLSGKMV*R*FINKLV IYKTINHL*FFL*FKWHFYAGSTAD*LIYCLDL TSYQIL*KENIYNI*LIWGK*SFDLFVFKCCCQT IVLRPPKCPILKELIHRKVFHFGVQPCHYVNAT SNWTSQDKWYQRPLKRCLNPFLEDRP*LK**QN VLLSGSWPSASELHINQISLYSLQ*ILIMASRLF GVGDAC*DLQVPS*KIETQSCSADSFGDTSIFL RDCFHLILAGPHQKIQPHTYIRQNIAVVPSVLK YCVLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI **NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR LHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEF KQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCV VENEYGSINHTYHLDVVERSPHRPILQAGLPAN ASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNG SKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTE ADAGEYICKVSNYIGQANQSAWLTVLPKQQAPG REKEITASPDYLEIAIYCIGVFLIACMVVTVIL CRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVS AESSSSMNSNTPLVRITTRLSSTADTPMLAGVS EYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVM AEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDL VSEMEMMKMIGKHKNIINLLGACTQDGPLYVIV EYASKGNLREYLRARRPPGMEYSYDINRVPEEQ MTFKDLVSCTYQLARGMEYLASQKCIHRDLAARN VLVTENNVMKIADFGLARDINNIDYYKKTTNGR LPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIF TLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNE LYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLT TNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSV FSPDPMPYEPCLPQYPHINGSVKT*MTVSACPQT GQHWEPSYTEQGDHASQSLLSPLVYMDQRSK*L EK*SAYV*RFIQLKTCNLPQEEKKVSGAVDCHK PPCNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**L EKIYVSTHLQSTNAVYRCWMYVNIFKLCINIYY IFTRSYFLY*F*MDVPMHLENWSLFF**LFAKC CSYT*FLNFHRAEVEKYFCFQGKWYNVNLLINW* YTKQLIIYSFFCNLSGISMQAAQQTS*SIAWT* LVIRSFEKRIFTIYD*FGENEVLIYLCLNAAVR RLFLDLLNAPY*KNSFIGRCFILVCNPVITSTQR LTGLPKINGTSVLLKDALIHSLRTDLS*NDSRM CFSLAAGLLLLSCTLIRLACILFSEF**WLPDS LALETPVRIFKSHHRKLKHRVVLLIVLGIRPSF* GIAFI*FWQDLTKRSSLIPTSDKISPLFLLY*S IVFCFGNTHSLCNSRAR*MQITLILCVTKLEKV FNKTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKEHHTGPTQKRWKSG SMLCLRPTLSSFAAQPGGTQCQPCGG*KTGRSL SRSIALEATRYETSTGASLWKVWSHLTREIIPV *WRMNTGPSITRTTWMLWSDRLTGPSSKPDCRQM PPQWSEET*SLSARFTVMPSPTSSGSSTWKRTA VNTGPTGCPTSRFSSTRG*IVPMQKCWLCSM*P RRMLGNIYVRSPII*GRPTSLPGSLSCQNSKRLE EKRRLQLPQTTWR*PFTA*GSS*SPVWW*QSSC AE*RTRPRSQTSAASRLCTS*PNVSPCGDR*QF RLSPAPP*TPTPRW*G*QHASLQRQTPPCWQGSP SMNFQRTQNGSFQEIS*HWASPWEKVALGKWSW RKQWELTKTSPRRRSPWP*RC*KMMPQRKTFLI WCQRWR**R*LGNTRIS*IFLEPAHRMGLSMS*L SMPLKATSENTSEPGGHPGWSTPMTLTVFLRSR *PSRTWCHAPTSWPEAWSTWLPKNVFIEI*QPE MFW*QKTM**K*QTLDSPEISTI*TITKRPPMGG FQSSGWLQKPCLIEYTLIRVMSGPSGC*CGRSS L*GARPTQGFPWRNFLSC*RKDTEWISQPTAPT NCT***GTVGMQCPPRDQRSSSW*KTWIEFSLSQ PMRNTWTSANLSNSIHLVTLTQEVLVLQEMILF FLQTPCLTNHAFLSIHT*TAVLKHE*LCLPVPK QDSTGNLATLSRETMPPRACCLHLYIWIRGVNNW KSNQHMCKDLYS*KLVIFPRRRRRFLEQWTATS HHVTPLTCRAYWLWTSRTQGGRAFCLPC*FCNN WRRFMSAHTYRAQMQYIGAGCM*IYSNYV*IYII YLQGVIFCIDFKWMSQCT*KIGLSFFNSYLLNA VLTHNFLIFTEQRWKNTFAFRENGITLIY**IG NIQNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN *LSDPLKREYLQYMTNLGKMKF*FICV*MLLSD DCS*TS*MPHIKRTHS*EGVSFWCATLSLRQRN V*LDFPR*MVPASS*KMP*SIP*GQTLVEMIAEC ASLWQLAFCF*VAH*SD*PVFSSVNFDNGFQTL WRWRRLLGSSSPIIEN*NTELFC**FWGYVHLF KGLLSSNSGRTSPKDPASYLHQTKYRRCSFCTKV LCFALETPTHFAIAVQDECRLH*SYVLQNWRKY LIKPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.mAug05

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.hAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGT GCGGAAGATTTTGTCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCA ACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGG CTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCC ATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAG ACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCT GCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATA TGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTT CCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAA GAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCC AACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAAT GGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAA GCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATG ATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAG AATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCAC CTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATG AAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAG CCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGAT TCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGG CATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCC AACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACC ATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACT GAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGAT TTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTG CGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTA CAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATT GATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCA CCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGT TTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAA TATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCC ATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGT ACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGC TTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTT CAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAG GACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACT CACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTG ACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRCHLIRR**G*HRW CGRFCQ*EQ*QQESTILDQHRKDGKAAPCCACGQ HCQVSLPSRGEPNANHAVAEKREGV*AGASHWR LQGTKPALEPHYGKCGPI*QGKLYLCSGE*IRV HQSHVPPGCCGAIASPAHPPSRTAGKCLHSGRRR RRVCLQGLQ*CPAPHPVDQARGKERQ*IRARRA ALPQGSQALGDK*FQCRSAGSVQCDRGGCWGIY M*GLQLYRAGQPVCLAHCPAKTASAWKRKGDYSF PRLPGDSHLLHRGLLNRLYGGNSHPVPNEEHDQ EARLQQPAGCAQADQTYPPAETGFG*VQLLHEL QHPAGEDNNTPLFNGRHPHAGRGLRV*TSRGPKM GVSKR*ADTGQAPGRRLLWASGHGGSSGN*QRQ AQGGGHRGREDVER*CHRERPF*SGVRDGDDED DWETQEYHKSSWSLHTGWASLCHS*VCL*RQPPR IPPSPEATRDGVLL*H*PCS*GADDLQGLGVMH LPAGQRHGVLGFPKMYSSRFSSQKCFGNRKQCD ENSRLWTRQRYQQYRLLQKDHQWAASSQVDGSRS PV**SIHSSE*CLVLRGVNVGDLHFRGLALPRD SRGGTF*AAEGRTQNG*ASQLHQRTVHDDEGLL ACSALPETNVQAVGRRLGSNSHSHNQ*GILGPQP TSRTVFT*LP*HKKFLFFRR*FCFFSRPHALRT MPSSVSTHKRQC*NMNDCVCLSPNRTALGT*LH *AGRPCLPELVVSTCIYGSEE*IIGKVISICVKI YTVENL*SSPGGEEGFWSSGLPQATM*PLSPAV RTGCGPVGLKVDVRSAFLVNFVIIGEDLCQHTL TEHKCSI*VLDVCKYIQIMYKYILYIYKELFFVL ILNGCPNAPRKLVSLFLIAIC*MLFLHIIS*FS PSRGGKILLLSGKMV*R*FINKLVIYKTINHL* FFL*FKWHFYAGSTAD*LIYCLDLTSYQIL*KEN IYNI*LIWGK*SFDLFVFKCCCQTIVLRPPKCP ILKELIHRKVFHFGVQPCHYVNATSNWTSQDKW YQRPLKRCLNPFLEDRP*LK**QNVLLSGSWPSA SELHINQISLYSLQ*ILIMASRLFGVGDAC*DL QVPS*KIETQSCSADSFGDTSIFLRDCFHLILA GPHQKIQPHTYIRQNIAVVPSVLKYCVLLWKHPL TLQ*PCKMNADYTDLMCYKIGESI**NLLIFIL TIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEDAISSGDDEDDTDG AEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAA NTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGG YKVRNQHWSLIMESVVPSDKGNYTCVVENEYGS INHTYHLDVVERSPHRPILQAGLPANASTVVGG DVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGL PYLKVLKHSGINSSNAEVLALFNVTEADAGEYI CKVSNYIGQANQSAWLTVLPKQQAPGREKEITA SPDYLEIAIYCIGVFLIACMVVTVILCRMKNTTK KPDFSSQPAVHKLTKRIPLRRQVSAESSSSMNS NTPLVRITTRLSSTADTPMLAGVSEYELPEDPK WEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDK PKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKM IGKHKNIINLLGACTQDGPLYVIVEYASKGNLR EYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCT YQLARGMEYLASQKCIHRDLAARNVLVTENNVM KIADFGLARDINNIDYYKKTTNGRLPVKWMAPE ALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGI PVEELFKLLKEGHRMDKPANCTNELYMMMRDCW HAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLS QPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEP CLPQYPHINGSVKT*MTVSACPQTGQHWEPSYT EQGDHASQSLLSPLVYMDQRSK*LEK*SAYV*R FIQLKTCNLPQEEKKVSGAVDCHKPPCNPSHLPC VLAVDQ*DSRWTCVLPSLLIL**LEKIYVSTHL QSTNAVYRCWMYVNIFKLCINIYYIFTRSYFLY *F*MDVPMHLENWSLFF**LFAKCCSYT*FLNFH RAEVEKYFCFQGKWYNVNLLINW*YTKQLIIYS FFCNLSGISMQAAQQTS*SIAWT*LVIRSFEKR IFTIYD*FGENEVLIYLCLNAAVRRLFLDLLNAP Y*KNSFIGRCFILVCNPVITSTQRLTGLPKING TSVLLKDALIHSLRTDLS*NDSRMCFSLAAGLL LLSCTLIRLACILFSEF**WLPDSLALETPVRIF KSHHRKLKHRVVLLIVLGIRPSF*GIAFI*FWQ DLTKRSSLIPTSDKISPLFLLY*SIVFCFGNTH SLCNSRAR*MQITLILCVTKLEKVFNKTC*FLY* Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKMPSHPEMMRMTPMV RKILSVRTVTTREHHTGPTQKRWKSGSMLCLRP TLSSFAAQPGGTQCQPCGG*KTGRSLSRSIALE ATRYETSTGASLWKVWSHLTREIIPV*WRMNTGP SITRTTWMLWSDRLTGPSSKPDCRQMPPQWSEE T*SLSARFTVMPSPTSSGSSTWKRTAVNTGPTG CPTSRFSSTRG*IVPMQKCWLCSM*PRRMLGNIY VRSPII*GRPTSLPGSLSCQNSKRLEEKRRLQL PQTTWR*PFTA*GSS*SPVWW*QSSCAE*RTRP RSQTSAASRLCTS*PNVSPCGDRFRLSPAPP*TP TPRW*G*QHASLQRQTPPCWQGSPSMNFQRTQN GSFQEIS*HWASPWEKVALGKWSWRKQWELTKT SPRRRSPWP*RC*KMMPQRKTFLIWCQRWR**R* LGNTRIS*IFLEPAHRMGLSMS*LSMPLKATSE NTSEPGGHPGWSTPMTLTVFLRSR*PSRTWCHA PTSWPEAWSTWLPKNVFIEI*QPEMFW*QKTM** K*QTLDSPEISTI*TITKRPPMGGFQSSGWLQK PCLIEYTLIRVMSGPSGC*CGRSSL*GARPTQG FPWRNFLSC*RKDTEWISQPTAPTNCT***GTVG MQCPPRDQRSSSW*KTWIEFSLSQPMRNTWTSA NLSNSIHLVTLTQEVLVLQEMILFFLQTPCLTN HAFLSIHT*TAVLKHE*LCLPVPKQDSTGNLATL SRETMPPRACCLHLYIWIRGVNNWKSNQHMCKD LYS*KLVIFPRRRRRFLEQWTATSHHVTPLTCR AYWLWTSRTQGGRAFCLPC*FCNNWRRFMSAHTY RAQMQYIGAGCM*IYSNYV*IYIIYLQGVIFCI DFKWMSQCT*KIGLSFFNSYLLNAVLTHNFLIF TEQRWKNTFAFRENGITLIY**IGNIQNN*SFIV FFVI*VAFLCRQHSRLVNLLLGLN*LSDPLKRE YLQYMTNLGKMKF*FICV*MLLSDDCS*TS*MP HIKRTHS*EGVSFWCATLSLRQRNV*LDFPR*MV PASS*KMP*SIP*GQTLVEMIAECASLWQLAFC F*VAH*SD*PVFSSVNFDNGFQTLWRWRRLLGS SSPIIEN*NTELFC**FWGYVHLFKGLLSSNSGR TSPKDPASYLHQTKYRRCSFCTKVLCFALETPT HFAIAVQDECRLH*SYVLQNWRKYLIKPVNFYT DNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.gAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGT GCGGAAGATTTTGTCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCA ACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGG CTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCC ATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAG ACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCT GCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAA TATACGTGCTTGGCGGGTAATTCTATTGGGATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCC CAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAA GCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAAC TCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAA AATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGA CAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAG ATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCC GAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATG CACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTG ATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAG AAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGG GATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGT TGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCA GCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGA ACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTAC ACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAA GATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCC GTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACAC TTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGT ATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTT TCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTAT AGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGA GAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGC CCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAAT GGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTC TGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGAT CTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGG CAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCC ACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATA CTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F14 (forward primer)
FGFR2.R14 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRCHLIRR**G*HRW CGRFCQ*EQ*QQESTILDQHRKDGKAAPCCACGQ HCQVSLPSRGEPNANHAVAEKREGV*AGASHWR LQGTKPALEPHYGKCGPI*QGKLYLCSGE*IRV HQSHVPPGCCGAIASPAHPPSRTAGKCLHSGRRR RRVCLQGLQ*CPAPHPVDQARGKERQ*IRARRA ALPQGSQGRRC*HHGQRD*GSLYSECNF*GRWG IYVLGG*FYWDILSLCMVDSSASAWKRKGDYSFP RLPGDSHLLHRGLLNRLYGGNSHPVPNEEHDQE ARLQQPAGCAQADQTYPPAETGNSFG*VQLLHE LQHPAGEDNNTPLFNGRHPHAGRGLRV*TSRGPK MGVSKR*ADTGQAPGRRLLWASGHGGSSGN*QR QAQGGGHRGREDVER*CHRERPF*SGVRDGDDE DDWETQEYHKSSWSLHTGWASLCHS*VCL*RQPP RIPPSPEATRDGVLL*H*PCS*GADDLQGLGVM HLPAGQRHGVLGFPKMYSSRFSSQKCFGNRKQC DENSRLWTRQRYQQYRLLQKDHQWAASSQVDGSR SPV**SIHSSE*CLVLRGVNVGDLHFRGLALPR DSRGGTF*AAEGRTQNG*ASQLHQRTVHDDEGL LACSALPETNVQAVGRRLGSNSHSHNQ*GILGPQ PTSRTVFT*LP*HKKFLFFRR*FCFFSRPHALR TMPSSVSTHKRQC*NMNDCVCLSPNRTALGT*L H*AGRPCLPELVVSTCIYGSEE*IIGKVISICVK IYTVENL*SSPGGEEGFWSSGLPQATM*PLSPA VRTGCGPVGLKVDVRSAFLVNFVIIGEDLCQHT LTEHKCSI*VLDVCKYIQIMYKYILYIYKELFFV LILNGCPNAPRKLVSLFLIAIC*MLFLHIIS*F SPSRGGKILLLSGKMV*R*FINKLVIYKTINHL *FFL*FKWHFYAGSTAD*LIYCLDLTSYQIL*KE NIYNI*LIWGK*SFDLFVFKCCCQTIVLRPPKC PILKELIHRKVFHFGVQPCHYVNATSNWTSQDK WYQRPLKRCLNPFLEDRP*LK**QNVLLSGSWPS ASELHINQISLYSLQ*ILIMASRLFGVGDAC*D LQVPS*KIETQSCSADSFGDTSIFLRDCFHLIL AGPHQKIQPHTYIRQNIAVVPSVLKYCVLLWKHP LTLQ*PCKMNADYTDLMCYKIGESI**NLLIFI LTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEDAISSGDDEDDTDG AEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAA NTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGG YKVRNQHWSLIMESVVPSDKGNYTCVVENEYGS INHTYHLDVVERSPHRPILQAGLPANASTVVGG DVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGL PYLKVLKAAGVNTTDKEIEVLYIRNVTFEDAGE YTCLAGNSIGISFHSAWLTVLPAPGREKEITAS PDYLEIAIYCIGVFLIACMVVTVILCRMKNTTKK PDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMN SNTPLVRITTRLSSTADTPMLAGVSEYELPEDP KWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKD KPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMK MIGKHKNIINLLGACTQDGPLYVIVEYASKGNL REYLRARRPPGMEYSYDINRVPEEQMTFKDLVSC TYQLARGMEYLASQKCIHRDLAARNVLVTENNV MKIADFGLARDINNIDYYKKTTNGRLPVKWMAP EALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPG IPVEELFKLLKEGHRMDKPANCTNELYMMMRDC WHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDL SQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYE PCLPQYPHINGSVKT*MTVSACPQTGQHWEPSY TEQGDHASQSLLSPLVYMDQRSK*LEK*SAYV* RFIQLKTCNLPQEEKKVSGAVDCHKPPCNPSHLP CVLAVDQ*DSRWTCVLPSLLIL**LEKIYVSTH LQSTNAVYRCWMYVNIFKLCINIYYIFTRSYFL Y*F*MDVPMHLENWSLFF**LFAKCCSYT*FLNF HRAEVEKYFCFQGKWYNVNLLINW*YTKQLIIY SFFCNLSGISMQAAQQTS*SIAWT*LVIRSFEK RIFTIYD*FGENEVLIYLCLNAAVRRLFLDLLNA PY*KNSFIGRCFILVCNPVITSTQRLTGLPKIN GTSVLLKDALIHSLRTDLS*NDSRMCFSLAAGL LLLSCTLIRLACILFSEF**WLPDSLALETPVRI FKSHHRKLKHRVVLLIVLGIRPSF*GIAFI*FW QDLTKRSSLIPTSDKISPLFLLY*SIVFCFGNT HSLCNSRAR*MQITLILCVTKLEKVFNKTC*FLY *Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKMPSHPEMMRMTPMV RKILSVRTVTTREHHTGPTQKRWKSGSMLCLRP TLSSFAAQPGGTQCQPCGG*KTGRSLSRSIALE ATRYETSTGASLWKVWSHLTREIIPV*WRMNTGP SITRTTWMLWSDRLTGPSSKPDCRQMPPQWSEE T*SLSARFTVMPSPTSSGSSTWKRTAVNTGPTG CPTSRFSRPPVLTPRTKRLRFSIFGM*LLRTLGN IRAWRVILLGYPFTLHG*QFCQRLEEKRRLQLP QTTWR*PFTA*GSS*SPVWW*QSSCAE*RTRPR SQTSAASRLCTS*PNVSPCGDR*QFRLSPAPP*T PTPRW*G*QHASLQRQTPPCWQGSPSMNFQRTQ NGSFQEIS*HWASPWEKVALGKWSWRKQWELTK TSPRRRSPWP*RC*KMMPQRKTFLIWCQRWR**R *LGNTRIS*IFLEPAHRMGLSMS*LSMPLKATS ENTSEPGGHPGWSTPMTLTVFLRSR*PSRTWCH APTSWPEAWSTWLPKNVFIEI*QPEMFW*QKTM* *K*QTLDSPEISTI*TITKRPPMGGFQSSGWLQ KPCLIEYTLIRVMSGPSGC*CGRSSL*GARPTQ GFPWRNFLSC*RKDTEWISQPTAPTNCT***GTV GMQCPPRDQRSSSW*KTWIEFSLSQPMRNTWTS ANLSNSIHLVTLTQEVLVLQEMILFFLQTPCLT NHAFLSIHT*TAVLKHE*LCLPVPKQDSTGNLAT LSRETMPPRACCLHLYIWIRGVNNWKSNQHMCK DLYS*KLVIFPRRRRRFLEQWTATSHHVTPLTC RAYWLWTSRTQGGRAFCLPC*FCNNWRRFMSAHT YRAQMQYIGAGCM*IYSNYV*IYIIYLQGVIFC IDFKWMSQCT*KIGLSFFNSYLLNAVLTHNFLI FTEQRWKNTFAFRENGITLIY**IGNIQNN*SFI VFFVI*VAFLCRQHSRLVNLLLGLN*LSDPLKR EYLQYMTNLGKMKF*FICV*MLLSDDCS*TS*M PHIKRTHS*EGVSFWCATLSLRQRNV*LDFPR*M VPASS*KMP*SIP*GQTLVEMIAECASLWQLAF CF*VAH*SD*PVFSSVNFDNGFQTLWRWRRLLG SSSPIIEN*NTELFC**FWGYVHLFKGLLSSNSG RTSPKDPASYLHQTKYRRCSFCTKVLCFALETP THFAIAVQDECRLH*SYVLQNWRKYLIKPVNFY TDNKNVSTDINVNKTK*MSRNLFF*