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Sequences

Sequence FGFR4.eAug05

FGFR4.F7 (forward primer)
FGFR4.R1 (reverse primer)
Alternative donor

Frame 0

RGGTGGGPLAATPPSRVHSSLPAEERSGCLRTL PRAGVAAGWSWE*GGGGAR*GGARKAVGGKSSLG P*EL*EGDAAAAGPVGGPAECAWASSLVPGGL* GSGA*LLDLQQR**GPQVP*GPLE*AQLPPAST LLDTPPAHGEETACSTCGEHRQVPLSSCRQPHAH HPLA*GWTGLSWGEPHWRHSAAPSALESRDGER GALGPRHIHLPGRERCGQHPL*LPARCAGAVPA PAHPAGRAPGQHHSRGGQRRGAAVQGVQRCPAPH PVAEAHRHQRQQLRSRRFPLCASPKDCRHQ*LR GGGPVPAERVSRGRRRVHLPRRQFHRPLLPVCL AHGAARGGPHMDRSSARGQVYGHHPVRVGLPGLG CAPAAGRAVSRAGAPRPAPPPARHCAEALPLPS GPTGTGRIPHLTCDSLTPAGRTKSPTLQFSLE

Frame 1

GVGQEVGRSRPRRRRGYIPRSRPRSARAVCGPC RVQGSRPAGAGSEAAEEPGEEEPGRQLVGSPAW VPESCEKEMRLLLALLGVLLSVPGPPVLSLEASE EVELDSLTSSNDDEDPKSHRDPSNRHSYPQQAP YWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTP TIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESV VPSDRGTYTCLVENAVGSIRYNYLLDVLERSPH RPILQAGLPANTTAVVGSDVELLCKVYSDAQPH IQWLKHIVINGSSFGADGFPYVQVLKTADINSSE VEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAW LTVLPEEDPTWTAAAPEARYTDIILYASGSLAL AVLLLLAGLYRGQALHGRHPRPPATVQKLSRFPL ARQVLGASPTSHVTA*LQQAEPSLPLCSSPW

Frame 2

GWDRRWAARGHAAVAGTFLAPGRGALGLSADPA ACRGRGRLELGVRRRRSQVRRSQEGSWWEVQLG SLRAVRRRCGCCWPCWGSC*VCLGLQSCPWRPL RKWSLTP*PPATMMRTPSPIGTPRIGTVTPSKHP TGHTPSAWRRNCMQYLRGTPSSSAVQLQATPRP PSAGLRMDRPFMGRTALEAFGCAISTGVS*WRA WCPRTAAHTPAW*RTLWAASAITTC*MCWSGPRT GPSCRPGSRPTPQPWWAATWSCCARCTAMPSPT SSG*STSSSTAAASEPTVSPMCKS*RLQTSIAQ RWRSCTCGTCQPRTQASTPASQAIPSASPTSLPG SRCCQRRTPHGPQQRPRPGIRTSSCTRRAPWPW LCSCCWPGCIEGRRSTAGTPARPPLCRSSPASL WPDRYWAHPPPHM*QPDSSRQNQVSHFAVLPG

Sequence FGFR4.aAug05

CGGGGTGGGACAGGAGGTGGGCCGCTCGCGGCCACGCCGCCGTCGCGGGTACATTCCTCGCTCCCGGCCGAGGAGCGCTCGGGCTGTCTGCGGACCCTGC CGCGTGCAGGGGTCGCGGCCGGCTGGAGCTGGGAGTGAGGCGGCGGAGGAGCCAGGTGAGGAGGAGCCAGGAAGGCAGTTGGTGGGAAGTCCAGCTTGGG TCCCTGAGAGCTGTGAGAAGGAGATGCGGCTGCTGCTGGCCCTGTTGGGGGTCCTGCTGAGTGTGCCTGGGCCTCCAGTCTTGTCCCTGGAGGCCTCTGA GGAAGTGGAGCTTGAGCCCTGCCTGGCTCCCAGCCTGGAGCAGCAAGAGCAGGAGCTGACAGTAGCCCTTGGGCAGCCTGTGCGTCTGTGCTGTGGGCGG GCTGAGCGTGGTGGCCACTGGTACAAGGAGGGCAGTCGCCTGGCACCTGCTGGCCGTGTACGGGGCTGGAGGGGCCGCCTAGAGATTGCCAGCTTCCTAC CTGAGGATGCTGGCCGCTACCTCTGCCTGGCACGAGGCTCCATGATCGTCCTGCAGAATCTCACCTTGATTACAGGTGACTCCTTGACCTCCAGCAACGA TGATGAGGACCCCAAGTCCCATAGGGACCCCTCGAATAGGCACAGTTACCCCCAGCAAGCACCCTACTGGACACACCCCCAGCGCATGGAGAAGAAACTG CATGCAGTACCTGCGGGGAACACCGTCAAGTTCCGCTGTCCAGCTGCAGGCAACCCCACGCCCACCATCCGCTGGCTTAAGGATGGACAGGCCTTTCATG GGGAGAACCGCATTGGAGGCATTCGGCTGCGCCATCAGCACTGGAGTCTCGTGATGGAGAGCGTGGTGCCCTCGGACCGCGGCACATACACCTGCCTGGT AGAGAACGCTGTGGGCAGCATCCGCTATAACTACCTGCTAGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCCGGGCTCCCGGCCAACACC ACAGCCGTGGTGGGCAGCGACGTGGAGCTGCTGTGCAAGGTGTACAGCGATGCCCAGCCCCACATCCAGTGGCTGAAGCACATCGTCATCAACGGCAGCA GCTTCGGAGCCGACGGTTTCCCCTATGTGCAAGTCCTAAAGACTGCAGACATCAATAGCTCAGAGGTGGAGGTCCTGTACCTGCGGAACGTGTCAGCCGA GGACGCAGGCGAGTACACCTGCCTCGCAGGCAATTCCATCGGCCTCTCCTACCAGTCTGCCTGGCTCACGGTGCTGCCAGAGGAGGACCCCACATGGACC GCAGCAGCGCCCGAGGCCAGGTATACGGACATCATCCTGTACGCGTCGGGCTCCCTGGCCTTGGCTGTGCTCCTGCTGCTGGCCGGGCTGTATCGAGGGC AGGCGCTCCACGGCCGGCACCCCCGCCCGCCCGCCACTGTGCAGAAGCTCTCCCGCTTCCCTCTGGCCCGACAGTTCTCCCTGGAGTCAGGCTCTTCCGG CAAGTCAAGCTCATCCCTGGTACGAGGCGTGCGTCTCTCCTCCAGCGGCCCCGCCTTGCTCGCCGGCCTCGTGAGTCTAGATCTACCTCTCGACCCACTA TGGGAGTTCCCCCGGGACAGGCTGGTGCTTGGGAAGCCCCTAGGCGAGGGCTGCTTTGGCCAGGTAGTACGTGCAGAGGCCTTTGGCATGGACCCTGCCC GGCCTGACCAAGCCAGCACTGTGGCCGTCAAGATGCTCAAAGACAACGCCTCTGACAAGGACCTGGCCGACCTGGTCTCGGAGATGGAGGTGATGAAGCT GATCGGCCGACACAAGAACATCATCAACCTGCTTGGTGTCTGCACCCAGGAAGGGCCCCTGTACGTGATCGTGGAGTGCGCCGCCAAGGGAAACCTGCGG GAGTTCCTGCGGGCCCGGCGCCCCCCAGGCCCCGACCTCAGCCCCGACGGTCCTCGGAGCAGTGAGGGGCCGCTCTCCTTCCCAGTCCTGGTCTCCTGCG CCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCCCGGAAGTGTATCCACCGGGACCTGGCTGCCCGCAATGTGCTGGTGACTGAGGACAATGTGAT GAAGATTGCTGACTTTGGGCTGGCCCGCGGCGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCCTGTGAAGTGGATGGCGCCCGAG GCCTTGTTTGACCGGGTGTACACACACCAGAGTGACGTGTGGTCTTTTGGGATCCTGCTATGGGAGATCTTCACCCTCGGGGGCTCCCCGTATCCTGGCA TCCCGGTGGAGGAGCTGTTCTCGCTGCTGCGGGAGGGACATCGGATGGACCGACCCCCACACTGCCCCCCAGAGCTGTACGGGCTGATGCGTGAGTGCTG GCACGCAGCGCCCTCCCAGAGGCCTACCTTCAAGCAGCTGGTGGAGGCGCTGGACAAGGTCCTGCTGGCCGTCTCTGAGGAGTACCTCGACCTCCGCCTG ACCTTCGGACCCTATTCCCCCTCTGGTGGGGACGCCAGCAGCACCTGCTCCTCCAGCGATTCTGTCTTCAGCCACGACCCCCTGCCATTGGGATCCAGCT CCTTCCCCTTCGGGTCTGGGGTGCAGACATGAGCAAGGCTCAAGGCTGTGCAGGCACATAGGCTGGTGGCCTTGGGCCTTGGGGCTCAGCCACAGCCTGA CACAGTGCTCGACCTTGATAGCATGGGGCCCCTGGCCCAGAGTTGCTGTGCCGTGTCCAAGGGCCGTGCCCTTGCCCTTGGAGCTGCCGTGCCTGTGTCC TGATGGCCCAAATGTCAGGGTTCTGCTCGGCTTCTTGGACCTTGGCGCTTAGTCCCCATCCCGGGTTTGGCTGAGCCTGGCTGGAGAGCTGCTATGCTAA ACCTCCTGCCTCCCAATACCAGCAGGAGGTTCTGGGCCTCTGAACCCCCTTTCCCCACACCTCCCCCTGCTGCTGCTGCCCCAGCGTCTTGACGGGAGCA TTGGCCCCTGAGCCCAGAGAAGCTGGAAGCCTGCCGAAAACAGGAGCAAATGGCGTTTTATAAATTATTTTTTTGAAATAAAGCTCTGTGTGCCTGGGTC

FGFR4.F7 (forward primer)
FGFR4.R1 (reverse primer)
Alternative donor

Frame 0

RGGTGGGPLAATPPSRVHSSLPAEERSGCLRTL PRAGVAAGWSWE*GGGGAR*GGARKAVGGKSSLG P*EL*EGDAAAAGPVGGPAECAWASSLVPGGL* GSGA*ALPGSQPGAARAGADSSPWAACASVLWA G*AWWPLVQGGQSPGTCWPCTGLEGPPRDCQLPT *GCWPLPLPGTRLHDRPAESHLDYR*LLDLQQR **GPQVP*GPLE*AQLPPASTLLDTPPAHGEET ACSTCGEHRQVPLSSCRQPHAHHPLA*GWTGLSW GEPHWRHSAAPSALESRDGERGALGPRHIHLPG RERCGQHPL*LPARCAGAVPAPAHPAGRAPGQH HSRGGQRRGAAVQGVQRCPAPHPVAEAHRHQRQQ LRSRRFPLCASPKDCRHQ*LRGGGPVPAERVSR GRRRVHLPRRQFHRPLLPVCLAHGAARGGPHMD RSSARGQVYGHHPVRVGLPGLGCAPAAGRAVSRA GAPRPAPPPARHCAEALPLPSGPTVLPGVRLFR QVKLIPGTRRASLLQRPRLARRPRESRSTSRPT MGVPPGQAGAWEAPRRGLLWPGSTCRGLWHGPCP A*PSQHCGRQDAQRQRL*QGPGRPGLGDGGDEA DRPTQEHHQPAWCLHPGRAPVRDRGVRRQGKPA GVPAGPAPPRPRPQPRRSSEQ*GAALLPSPGLLR LPGGPRHAVSGVPEVYPPGPGCPQCAGD*GQCD EDC*LWAGPRRPPH*LL*ENQQRPPACEVDGAR GLV*PGVHTPE*RVVFWDPAMGDLHPRGLPVSWH PGGGAVLAAAGGTSDGPTPTLPPRAVRADA*VL ARSALPEAYLQAAGGGAGQGPAGRL*GVPRPPP DLRTLFPLWWGRQQHLLLQRFCLQPRPPAIGIQL LPLRVWGADMSKAQGCAGT*AGGLGPWGSATA* HSARP**HGAPGPELLCRVQGPCPCPWSCRACV LMAQMSGFCSASWTLALSPHPGFG*AWLESCYAK PPASQYQQEVLGL*TPFPHTSPCCCCPSVLTGA LAPEPREAGSLPKTGANGVL*IIFLK*SSVCLG

Frame 1

GVGQEVGRSRPRRRRGYIPRSRPRSARAVCGPC RVQGSRPAGAGSEAAEEPGEEEPGRQLVGSPAW VPESCEKEMRLLLALLGVLLSVPGPPVLSLEASE EVELEPCLAPSLEQQEQELTVALGQPVRLCCGR AERGGHWYKEGSRLAPAGRVRGWRGRLEIASFL PEDAGRYLCLARGSMIVLQNLTLITGDSLTSSND DEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKL HAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFH GENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLV ENAVGSIRYNYLLDVLERSPHRPILQAGLPANT TAVVGSDVELLCKVYSDAQPHIQWLKHIVINGS SFGADGFPYVQVLKTADINSSEVEVLYLRNVSAE DAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWT AAAPEARYTDIILYASGSLALAVLLLLAGLYRG QALHGRHPRPPATVQKLSRFPLARQFSLESGSSG KSSSSLVRGVRLSSSGPALLAGLVSLDLPLDPL WEFPRDRLVLGKPLGEGCFGQVVRAEAFGMDPA RPDQASTVAVKMLKDNASDKDLADLVSEMEVMKL IGRHKNIINLLGVCTQEGPLYVIVECAAKGNLR EFLRARRPPGPDLSPDGPRSSEGPLSFPVLVSC AYQVARGMQYLESRKCIHRDLAARNVLVTEDNVM KIADFGLARGVHHIDYYKKTSNGRLPVKWMAPE ALFDRVYTHQSDVWSFGILLWEIFTLGGSPYPG IPVEELFSLLREGHRMDRPPHCPPELYGLMRECW HAAPSQRPTFKQLVEALDKVLLAVSEEYLDLRL TFGPYSPSGGDASSTCSSSDSVFSHDPLPLGSS SFPFGSGVQT*ARLKAVQAHRLVALGLGAQPQPD TVLDLDSMGPLAQSCCAVSKGRALALGAAVPVS *WPKCQGSARLLGPWRLVPIPGLAEPGWRAAML NLLPPNTSRRFWASEPPFPTPPPAAAAPAS*REH WPLSPEKLEACRKQEQMAFYKLFF*NKALCAWV

Frame 2

GWDRRWAARGHAAVAGTFLAPGRGALGLSADPA ACRGRGRLELGVRRRRSQVRRSQEGSWWEVQLG SLRAVRRRCGCCWPCWGSC*VCLGLQSCPWRPL RKWSLSPAWLPAWSSKSRS*Q*PLGSLCVCAVGG LSVVATGTRRAVAWHLLAVYGAGGAA*RLPASY LRMLAATSAWHEAP*SSCRISP*LQVTP*PPAT MMRTPSPIGTPRIGTVTPSKHPTGHTPSAWRRNC MQYLRGTPSSSAVQLQATPRPPSAGLRMDRPFM GRTALEAFGCAISTGVS*WRAWCPRTAAHTPAW *RTLWAASAITTC*MCWSGPRTGPSCRPGSRPTP QPWWAATWSCCARCTAMPSPTSSG*STSSSTAA ASEPTVSPMCKS*RLQTSIAQRWRSCTCGTCQP RTQASTPASQAIPSASPTSLPGSRCCQRRTPHGP QQRPRPGIRTSSCTRRAPWPWLCSCCWPGCIEG RRSTAGTPARPPLCRSSPASLWPDSSPWSQALP ASQAHPWYEACVSPPAAPPCSPAS*V*IYLSTHY GSSPGTGWCLGSP*ARAALAR*YVQRPLAWTLP GLTKPALWPSRCSKTTPLTRTWPTWSRRWR**S *SADTRTSSTCLVSAPRKGPCT*SWSAPPRETCG SSCGPGAPQAPTSAPTVLGAVRGRSPSQSWSPA PTRWPEACSIWSPGSVSTGTWLPAMCW*LRTM* *RLLTLGWPAASTTLTTIRKPATAACL*SGWRPR PCLTGCTHTRVTCGLLGSCYGRSSPSGAPRILA SRWRSCSRCCGRDIGWTDPHTAPQSCTG*CVSA GTQRPPRGLPSSSWWRRWTRSCWPSLRSTSTSA* PSDPIPPLVGTPAAPAPPAILSSATTPCHWDPA PSPSGLGCRHEQGSRLCRHIGWWPWALGLSHSL TQCSTLIAWGPWPRVAVPCPRAVPLPLELPCLCP DGPNVRVLLGFLDLGA*SPSRVWLSLAGELLC* TSCLPIPAGGSGPLNPLSPHLPLLLLPQRLDGS IGP*AQRSWKPAENRSKWRFINYFFEIKLCVPG

Sequence FGFR4.gAug05

FGFR4.F7 (forward primer)
FGFR4.R1 (reverse primer)
Alternative donor

Frame 0

RSRPRRRRGYIPRSRPRSARAVCGPCRVQGSRP AGAGSEAAEEPGEEEPGEQDPVLGAESRRLEEGS WWEVQLGSLRAVRRRCGCCWPCWGSC*VCLGLQ SCPWRPLRKWSLVVRDSSRE*RDAWGDRPAPLG P*MLPSVPDVDSLTSSNDDEDPKSHRDPSNRHSY PQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAA GNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWS LVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVL ERSPHRPILQAGLPANTTAVVGSDVELLCKVYS DAQPHIQ

Frame 1

ARGHAAVAGTFLAPGRGALGLSADPAACRGRGR LELGVRRRRSQVRRSQVSRTLCWARSHAGSRKA VGGKSSLGP*EL*EGDAAAAGPVGGPAECAWASS LVPGGL*GSGAWW*ETLAGSEGMPGETDLPLLD LRCFPLSLM*TP*PPATMMRTPSPIGTPRIGTV TPSKHPTGHTPSAWRRNCMQYLRGTPSSSAVQLQ ATPRPPSAGLRMDRPFMGRTALEAFGCAISTGV S*WRAWCPRTAAHTPAW*RTLWAASAITTC*MC WSGPRTGPSCRPGSRPTPQPWWAATWSCCARCTA MPSPTSS

Frame 2

LAATPPSRVHSSLPAEERSGCLRTLPRAGVAAG WSWE*GGGGAR*GGAR*AGPCAGRGVTQARGRQ LVGSPAWVPESCEKEMRLLLALLGVLLSVPGPP VLSLEASEEVELGGKRL*QGVKGCLGRQTCPSWT LDASLCP*CRLLDLQQR**GPQVP*GPLE*AQL PPASTLLDTPPAHGEETACSTCGEHRQVPLSSC RQPHAHHPLA*GWTGLSWGEPHWRHSAAPSALES RDGERGALGPRHIHLPGRERCGQHPL*LPARCA GAVPAPAHPAGRAPGQHHSRGGQRRGAAVQGVQ RCPAPHPV

Sequence FGFR4.bAug05

CGCTCGCGGCCACGCCGCCGTCGCGGGTACATTCCTCGCTCCCGGCCGAGGAGCGCTCGGGCTGTCTGCGGACCCTGCCGCGTGCAGGGGTCGCGGCCGG CTGGAGCTGGGAGTGAGGCGGCGGAGGAGCCAGGTGAGGAGGAGCCAGGTGAGCAGGACCCTGTGCTGGGCGCGGAGTCACGCAGGCTCGAGGAAGGCAG TTGGTGGGAAGTCCAGCTTGGGTCCCTGAGAGCTGTGAGAAGGAGATGCGGCTGCTGCTGGCCCTGTTGGGGGTCCTGCTGAGTGTGCCTGGGCCTCCAG TCTTGTCCCTGGAGGCCTCTGAGGAAGTGGAGCTTGAGCCCTGCCTGGCTCCCAGCCTGGAGCAGCAAGAGCAGGAGCTGACAGTAGCCCTTGGGCAGCC TGTGCGTCTGTGCTGTGGGCGGGCTGAGCGTGGTGGCCACTGGTACAAGGAGGGCAGTCGCCTGGCACCTGCTGGCCGTGTACGGGGCTGGAGGGGCCGC CTAGAGATTGCCAGCTTCCTACCTGAGGATGCTGGCCGCTACCTCTGCCTGGCACGAGGCTCCATGATCGTCCTGCAGAATCTCACCTTGATTACAGGTG ACTCCTTGACCTCCAGCAACGATGATGAGGACCCCAAGTCCCATAGGGACCCCTCGAATAGGCACAGTTACCCCCAGCAAGCACCCTACTGGACACACCC CCAGCGCATGGAGAAGAAACTGCATGCAGTACCTGCGGGGAACACCGTCAAGTTCCGCTGTCCAGCTGCAGGCAACCCCACGCCCACCATCCGCTGGCTT AAGGATGGACAGGCCTTTCATGGGGAGAACCGCATTGGAGGCATTCGGCTGCGCCATCAGCACTGGAGTCTCGTGATGGAGAGCGTGGTGCCCTCGGACC GCGGCACATACACCTGCCTGGTAGAGAACGCTGTGGGCAGCATCCGCTATAACTACCTGCTAGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCA GGCCGGGCTCCCGGCCAACACCACAGCCGTGGTGGGCAGCGACGTGGAGCTGCTGTGCAAGGTGTACAGCGATGCCCAGCCCCACATCCAGTGGCTGAAG CACATCGTCATCAACGGCAGCAGCTTCGGAGCCGACGGTTTCCCCTATGTGCAAGTCCTAAAGACTGCAGACATCAATAGCTCAGAGGTGGAGGTCCTGT ACCTGCGGAACGTGTCAGCCGAGGACGCAGGCGAGTACACCTGCCTCGCAGGCAATTCCATCGGCCTCTCCTACCAGTCTGCCTGGCTCACGGTGCTGCC AGAGGAGGACCCCACATGGACCGCAGCAGCGCCCGAGGCCAGGTATACGGACATCATCCTGTACGCGTCGGGCTCCCTGGCCTTGGCTGTGCTCCTGCTG CTGGCCGGGCTGTATCGAGGGCAGGCGCTCCACGGCCGGCACCCCCGCCCGCCCGCCACTGTGCAGAAGCTCTCCCGCTTCCCTCTGGCCCGACAGTTCT CCCTGGAGTCAGGCTCTTCCGGCAAGTCAAGCTCATCCCTGGTACGAGGCGTGCGTCTCTCCTCCAGCGGCCCCGCCTTGCTCGCCGGCCTCGTGAGTCT AGATCTACCTCTCGACCCACTATGGGAGTTCCCCCGGGACAGGCTGGTGCTTGGGAAGCCCCTAGGCGAGGGCTGCTTTGGCCAGGTAGTACGTGCAGAG GCCTTTGGCATGGACCCTGCCCGGCCTGACCAAGCCAGCACTGTGGCCGTCAAGATGCTCAAAGACAACGCCTCTGACAAGGACCTGGCCGACCTGGTCT CGGAGATGGAGGTGATGAAGCTGATCGGCCGACACAAGAACATCATCAACCTGCTTGGTGTCTGCACCCAGGAAGGGCCCCTGTACGTGATCGTGGAGTG CGCCGCCAAGGGAAACCTGCGGGAGTTCCTGCGGGCCCGGCGCCCCCCAGGCCCCGACCTCAGCCCCGACGGTCCTCGGAGCAGTGAGGGGCCGCTCTCC TTCCCAGTCCTGGTCTCCTGCGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCCCGGAAGTGTATCCACCGGGACCTGGCTGCCCGCAATGTGC TGGTGACTGAGGACAATGTGATGAAGATTGCTGACTTTGGGCTGGCCCGCGGCGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCC TGTGAAGTGGATGGCGCCCGAGGCCTTGTTTGACCGGGTGTACACACACCAGAGTGACGTGTGGTCTTTTGGGATCCTGCTATGGGAGATCTTCACCCTC GGGGGCTCCCCGTATCCTGGCATCCCGGTGGAGGAGCTGTTCTCGCTGCTGCGGGAGGGACATCGGATGGACCGACCCCCACACTGCCCCCCAGAGCTGT ACGGGCTGATGCGTGAGTGCTGGCACGCAGCGCCCTCCCAGAGGCCTACCTTCAAGCAGCTGGTGGAGGCGCTGGACAAGGTCCTGCTGGCCGTCTCTGA GGAGTACCTCGACCTCCGCCTGACCTTCGGACCCTATTCCCCCTCTGGTGGGGACGCCAGCAGCACCTGCTCCTCCAGCGATTCTGTCTTCAGCCACGAC CCCCTGCCATTGGGATCCAGCTCCTTCCCCTTCGGGTCTGGGGTGCAGACATGAGCAAGGCTCAAGGCTGTGCAGGCACATAGGCTGGTGGCCTTGGGCC TTGGGGCTCAGCCACAGCCTGACACAGTGCTCGACCTTGATAGCATGGGGCCCCTGGCCCAGAGTTGCTGTGCCGTGTCCAAGGGCCGTGCCCTTGCCCT TGGAGCTGCCGTGCCTGTGTCCTGATGGCCCAAATGTCAGGGTTCTGCTCGGCTTCTTGGACCTTGGCGCTTAGTCCCCATCCCGGGTTTGGCTGAGCCT GGCTGGAGAGCTGCTATGCTAAACCTCCTGCCTCCCAATACCAGCAGGAGGTTCTGGGCCTCTGAACCCCCTTTCCCCACACCTCCCCCTGCTGCTGCTG CCCCAGCGTCTTGACGGGAGCATTGGCCCCTGAGCCCAGAGAAGCTGGAAGCCTGCCGAAAACAGGAGCAAATGGCGTTTTATAAATTATTTTTTTGAAA TAAAGCTCTGTGTGCCTGGGTC

FGFR4.F7 (forward primer)
FGFR4.R1 (reverse primer)
Alternative donor

Frame 0

RSRPRRRRGYIPRSRPRSARAVCGPCRVQGSRP AGAGSEAAEEPGEEEPGEQDPVLGAESRRLEEGS WWEVQLGSLRAVRRRCGCCWPCWGSC*VCLGLQ SCPWRPLRKWSLSPAWLPAWSSKSRS*Q*PLGS LCVCAVGGLSVVATGTRRAVAWHLLAVYGAGGAA *RLPASYLRMLAATSAWHEAP*SSCRISP*LQV TP*PPATMMRTPSPIGTPRIGTVTPSKHPTGHT PSAWRRNCMQYLRGTPSSSAVQLQATPRPPSAGL RMDRPFMGRTALEAFGCAISTGVS*WRAWCPRT AAHTPAW*RTLWAASAITTC*MCWSGPRTGPSC RPGSRPTPQPWWAATWSCCARCTAMPSPTSSG*S TSSSTAAASEPTVSPMCKS*RLQTSIAQRWRSC TCGTCQPRTQASTPASQAIPSASPTSLPGSRCC QRRTPHGPQQRPRPGIRTSSCTRRAPWPWLCSCC WPGCIEGRRSTAGTPARPPLCRSSPASLWPDSS PWSQALPASQAHPWYEACVSPPAAPPCSPAS*V *IYLSTHYGSSPGTGWCLGSP*ARAALAR*YVQR PLAWTLPGLTKPALWPSRCSKTTPLTRTWPTWS RRWR**S*SADTRTSSTCLVSAPRKGPCT*SWS APPRETCGSSCGPGAPQAPTSAPTVLGAVRGRSP SQSWSPAPTRWPEACSIWSPGSVSTGTWLPAMC W*LRTM**RLLTLGWPAASTTLTTIRKPATAAC L*SGWRPRPCLTGCTHTRVTCGLLGSCYGRSSPS GAPRILASRWRSCSRCCGRDIGWTDPHTAPQSC TG*CVSAGTQRPPRGLPSSSWWRRWTRSCWPSL RSTSTSA*PSDPIPPLVGTPAAPAPPAILSSATT PCHWDPAPSPSGLGCRHEQGSRLCRHIGWWPWA LGLSHSLTQCSTLIAWGPWPRVAVPCPRAVPLP LELPCLCPDGPNVRVLLGFLDLGA*SPSRVWLSL AGELLC*TSCLPIPAGGSGPLNPLSPHLPLLLL PQRLDGSIGP*AQRSWKPAENRSKWRFINYFFE IKLCVPG

Frame 1

ARGHAAVAGTFLAPGRGALGLSADPAACRGRGR LELGVRRRRSQVRRSQVSRTLCWARSHAGSRKA VGGKSSLGP*EL*EGDAAAAGPVGGPAECAWASS LVPGGL*GSGA*ALPGSQPGAARAGADSSPWAA CASVLWAG*AWWPLVQGGQSPGTCWPCTGLEGP PRDCQLPT*GCWPLPLPGTRLHDRPAESHLDYR* LLDLQQR**GPQVP*GPLE*AQLPPASTLLDTP PAHGEETACSTCGEHRQVPLSSCRQPHAHHPLA *GWTGLSWGEPHWRHSAAPSALESRDGERGALGP RHIHLPGRERCGQHPL*LPARCAGAVPAPAHPA GRAPGQHHSRGGQRRGAAVQGVQRCPAPHPVAE AHRHQRQQLRSRRFPLCASPKDCRHQ*LRGGGPV PAERVSRGRRRVHLPRRQFHRPLLPVCLAHGAA RGGPHMDRSSARGQVYGHHPVRVGLPGLGCAPA AGRAVSRAGAPRPAPPPARHCAEALPLPSGPTVL PGVRLFRQVKLIPGTRRASLLQRPRLARRPRES RSTSRPTMGVPPGQAGAWEAPRRGLLWPGSTCR GLWHGPCPA*PSQHCGRQDAQRQRL*QGPGRPGL GDGGDEADRPTQEHHQPAWCLHPGRAPVRDRGV RRQGKPAGVPAGPAPPRPRPQPRRSSEQ*GAAL LPSPGLLRLPGGPRHAVSGVPEVYPPGPGCPQCA GD*GQCDEDC*LWAGPRRPPH*LL*ENQQRPPA CEVDGARGLV*PGVHTPE*RVVFWDPAMGDLHP RGLPVSWHPGGGAVLAAAGGTSDGPTPTLPPRAV RADA*VLARSALPEAYLQAAGGGAGQGPAGRL* GVPRPPPDLRTLFPLWWGRQQHLLLQRFCLQPR PPAIGIQLLPLRVWGADMSKAQGCAGT*AGGLGP WGSATA*HSARP**HGAPGPELLCRVQGPCPCP WSCRACVLMAQMSGFCSASWTLALSPHPGFG*A WLESCYAKPPASQYQQEVLGL*TPFPHTSPCCCC PSVLTGALAPEPREAGSLPKTGANGVL*IIFLK *SSVCLG

Frame 2

LAATPPSRVHSSLPAEERSGCLRTLPRAGVAAG WSWE*GGGGAR*GGAR*AGPCAGRGVTQARGRQ LVGSPAWVPESCEKEMRLLLALLGVLLSVPGPP VLSLEASEEVELEPCLAPSLEQQEQELTVALGQP VRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGR LEIASFLPEDAGRYLCLARGSMIVLQNLTLITG DSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHP QRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWL KDGQAFHGENRIGGIRLRHQHWSLVMESVVPSD RGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQ AGLPANTTAVVGSDVELLCKVYSDAQPHIQWLK HIVINGSSFGADGFPYVQVLKTADINSSEVEVL YLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLP EEDPTWTAAAPEARYTDIILYASGSLALAVLLL LAGLYRGQALHGRHPRPPATVQKLSRFPLARQF SLESGSSGKSSSSLVRGVRLSSSGPALLAGLVSL DLPLDPLWEFPRDRLVLGKPLGEGCFGQVVRAE AFGMDPARPDQASTVAVKMLKDNASDKDLADLV SEMEVMKLIGRHKNIINLLGVCTQEGPLYVIVEC AAKGNLREFLRARRPPGPDLSPDGPRSSEGPLS FPVLVSCAYQVARGMQYLESRKCIHRDLAARNV LVTEDNVMKIADFGLARGVHHIDYYKKTSNGRLP VKWMAPEALFDRVYTHQSDVWSFGILLWEIFTL GGSPYPGIPVEELFSLLREGHRMDRPPHCPPEL YGLMRECWHAAPSQRPTFKQLVEALDKVLLAVSE EYLDLRLTFGPYSPSGGDASSTCSSSDSVFSHD PLPLGSSSFPFGSGVQT*ARLKAVQAHRLVALG LGAQPQPDTVLDLDSMGPLAQSCCAVSKGRALAL GAAVPVS*WPKCQGSARLLGPWRLVPIPGLAEP GWRAAMLNLLPPNTSRRFWASEPPFPTPPPAAA APAS*REHWPLSPEKLEACRKQEQMAFYKLFF*N KALCAWV