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Sequences

Sequence SLIT2.bAug05

TGCCCCGCGCGCTCTGCTACGGGCCCGCTGGGCTTCCGCGCCTTCTAGCTTCCGGAGCCCACTTTGATCGGGGCCATAATACCTATTGAGATCCCCTCTT CTGTCTTGTACCTTCGCCACTGGCATCGGATTTGCAGAAGCGTGCGTGGGATCAGAGGACCGCCCTCCCCACAACAACCGGCCCCTGCATCTTAGCAGCC GTTGGAAGCCCCAGCTCTTTTACCGCCAAGTTCATCCTTGGGAGACAGAAGACGCGTGATCTCCTCTCCGCTGCTCTTGGGGTCTCCTTGCAGCCCTGGC CAGGCGGATTCATCCTCAGGACCTAAAGTTGCCCAAGGAGCTCCTGCTCTGCCAGAGGAGGGTGGAGAGGGCGGTGGGAGGCGTGTGCCTGAGTGGGCTC TACTGCCTTGTTCCATATTATTTGGTGCACATTTTCCCTGGCACTCTGGGTTGCTAGCCCCGCCGGGCACTGGGCCTCAGACACTGCGCGGTTCCCTCGG AGCAGCAAGCTAAAGAAAGCCCCCAGTGCCGGCGAGGAAGGAGGCGGCGGGGAAAGATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTT AGTGCTGGCGATCCTGAACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCGCAGC GTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATTACGAAGACAGATTTTGCTGGTCTTAGACATCTAA GAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCATTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCT TCAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAAATTCAGGCAATCCCAAGGAAAGCTTTCCGT GGGGCAGTTGACATAAAAAATTTGCAACTGGATTACAACCAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTCACTC TCAACAATAACAACATTACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCATTCAAACAACCTGTATTGTGACTG CCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACACTCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCC GAGGTTCAAAAACGAGAATTTGTCTGCAGTGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCCTGTACCTGTAGCAACA ATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCAGAGACCATCACAGAAATACGTTTGGAACAGAACACAATCAAAGTCAT CCCTCCTGGAGCTTTCTCACCATATAAAAAGCTTAGACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCACCAGATGCTTTCCAAGGACTACGC TCTCTGAATTCACTTGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTCCTTACAGCTCCTATTATTGAATGCCA ACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGATCTCCACAACTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAAGGGGAC CTTTTCACCTCTTCGGGCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCATCTCAAGTGGCTAGCGGATTATCTCCATACCAAC CCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAG AAGATTATCGATCAAAATTAAGTGGAGACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGAACCACAGTAGATTGCTCTAATCAAAA GCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAGTTGCGTCTCAATAATAATGAATTTACCGTGTTGGAAGCCACAGGAATCTTTAAGAAA CTTCCTCAATTACGTAAAATAAACTTTAGCAACAATAAGATCACAGATATTGAGGAGGGAGCATTTGAAGGAGCATCTGGTGTAAATGAAATACTTCTTA CGAGTAATCGTTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTTGAGAAGCAATCGAATAACCTGTGTGGGGAA TGACAGTTTCATAGGACTCAGTTCTGTGCGTTTGCTTTCTTTGTATGATAATCAAATTACTACAGTTGCACCAGGGGCATTTGATACTCTCCATTCTTTA TCTACTCTAAACCTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTGGGAGAGTGGCTGAGAAAGAAGAGAATTGTCACGGGAAATCCTA GATGTCAAAAACCATACTTCCTGAAAGAAATACCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGATGACGGAAATGATGACAATAGTTGCTCCCC ACTTTCTCGCTGTCCTACTGAATGTACTTGCTTGGATACAGTCGTCCGATGTAGCAACAAGGGTTTGAAGGTCTTGCCGAAAGGTATTCCAAGAGATGTC ACAGAGTTGTATCTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACTCTCCAACTACAAACATTTAACACTTATAGACTTAAGTAACAACAGAATAA GCACGCTTTCTAATCAGAGCTTCAGCAACATGACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCTGAGATGTATTCCTCCTCGCACCTTTGATGG ATTAAAGTCTCTTCGATTACTTTCTCTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTTTCTGCATTATCACATCTAGCAATT GGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAGCCTGGAATTGCTCGTTGTGCTGGTCCTG GAGAAATGGCAGATAAACTTTTACTCACAACTCCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGTCAATATTCTAGCTAAGTGTAACCCCTGCCT ATCAAATCCGTGTAAAAATGATGGCACATGTAATAGTGATCCAGTTGACTTTTACCGATGCACCTGTCCATATGGTTTCAAGGGGCAGGACTGTGATGTC CCAATTCATGCCTGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCACTTAAAGGAAGGAGAAGAAGATGGATTCTGGTGTATTTGTGCTGATGGAT TTGAAGGAGAAAATTGTGAAGTCAACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTACATGTGTCGATGGCATTAATAACTACACATGCCT TTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGAGAAGCTGGACTTCTGTGCCCAGGACCTGAACCCCTGCCAGCACGATTCAAAGTGCATCCTAACT CCAAAGGGATTCAAATGTGACTGCACACCAGGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGCCAAGACAACAAGTGTAAAAACGGAGCCC ACTGCACAGATGCAGTGAACGGCTATACGTGCATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGTACCAG CCCCTGTGATAATTTTGATTGTCAGAATGGAGCTCAGTGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTGCCTGGCTATCAGGGAGAAAAGTGT GAAAAATTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTCAGCCAAGGTTCGGCCTCAGACGAACATAACACTTCAGATTGCCA CAGATGAAGACAGCGGAATCCTCCTGTATAAGGGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGGGCGTGTTCGTGCCAGCTATGACACCGGCTC TCATCCAGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAACTACTTGCCTTGGATCAGAGTCTCTCTTTGTCCGTG GATGGTGGGAACCCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCTGAATTTTGACTCTCCACTCTATGTAGGAGGCATGCCAGGGAAGAGTAACG TGGCATCTCTGCGCCAGGCCCCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAGTGAGCTGCAGGACTTCCAGAAGGT GCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAGAAGGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAGGCAGGCTTCACCTGC GAGTGCCAGGAAGGATGGATGGGGCCCCTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGCGTACATGGCACCTGCTTGCCCATCAATG CGTTCTCCTACAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAAGAGGAGGATCTGTTTAACCCATGCCAGGCGATCAAGTGCAAGCA TGGGAAGTGCAGGCTTTCAGGTCTGGGGCAGCCCTACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGATCGAGAAATCTCTTGTCGAGGGGAA AGGATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCCCGATTAGAGTGCAGAGGTGGGTGTGCAGGAGGGC AGTGCTGTGGACCGCTGAGGAGCAAGCGGCGGAAATACTCTTTCGAATGCACTGACGGCTCCTCCTTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGG CTGTACGAGGTGTGTGTCCTAAACACACTCCCGGCAGCTCTGTCTTTGGAAAAGGTTGTATACTTCTTGACCGTGTGGGACTAATGAATGCTTCATAGTG GAAATATTTGAAATATATTGTAAAATACAGAACAGACTTATTTTTATTATGAGAATAAAGACTTTTTTTCTGCATTTGGAAAAAAAAAAAAAGAAATGCT TGAACTAAAGCTTCCCCTATGCTGGAGAAGTATGAAGAAAGATATACCTGGAGACATTAGAACAGCGATGGGAACCATTGCAACTCGGGTCCATCTTTGT AACATGCTGAAGACAAGCAGAAGCACATGCACGAGGGACAGAGGAGCTACTGTGCACTGCTGTGAAATTGCCCAGAGCATAAAACCTGTGTACCCTCCTT CACATCAACCAAGTTCACTAGGACATACCAAGCACATGCGTGTGAATGAGGATGCAAGGCAAGAGAATGGAACTCCAGAATTCACAGATAGAACAAATGG ATGAGAAATATTTCATGCAAAATATAAAGTGTCCTGAGGATCTGGGTTCCATTTACGAGAGGAAATGAAAGTGCTAAAATAAATTTTATCTTCCTTTTAA ATGTCAGCATGTCAGCAGAAGCAGCACACAAAAGTCTTTACCATTTTCCAGTATTAATTTTTTTGTAATATAAATGATAAAGGAGATGATAAAGAACCAA TAGATTATTGATAAATAAATTAGTAATAATA

SLIT2.F36 (forward primer)
SLIT2.R4 (reverse primer)
Single exon skipping

Frame 0

CPARSATGPLGFRAF*LPEPTLIGAIIPIEIPS SVLYLRHWHRICRSVRGIRGPPSPQQPAPAS*QP LEAPALLPPSSSLGDRRRVISSPLLLGSPCSPG QADSSSGPKVAQGAPALPEEGGEGGGRRVPEWA LLPCSILFGAHFPWHSGLLAPPGTGPQTLRGSLG AAS*RKPPVPARKEAAGKDARRWLADAVPVAGV SAGDPEQGGTAGVPGAVLLLGQHSGLSRAGAAQ RAQEYPPQHRETGFKWK*HHKNYEDRFCWS*TSK SSSAYGE*D*HH*KRSIPGS*RTRETAFKQKSP SAVS*VAVSWDCEAIQA*SQ*KPNSGNPKESFP WGS*HKKFATGLQPDQLY*RWGIQGSPGPGSAHS QQ*QHY*TFCGKFQPYA*T*DFSTAFKQPVL*L PPGLALRLASPKASGWSVHSVYGPLPPERP*CS RGSKTRICLQWSPVIYGSFL*CFALPCRLYL*QQ YRRLSWERSH*DPHKSSRDHHRNTFGTEHNQSH PSWSFLTI*KA*TN*PEQ*SDL*TCTRCFPRTT LSEFTCPLWK*NHRTPQKFI*RTVFLTAPIIECQ QDKLPSGRCFSGSPQLEPSLPI*QQASDHRQGD LFTSSGHSNYAFGPEPLYL*LPSQVASGLSPYQ PD*DQWCPLHQPPPPGKQKNWTDQKQEIPLFRYR RLSIKIKWRLLCGSGLP*KVSL*RNHSRLL*SK AQQNPGAHSPVHCRVASQ***IYRVGSHRNL*E TSSIT*NKL*QQ*DHRY*GGSI*RSIWCK*NTSY E*SFGKCAA*DVQGIGKPQNFDVEKQSNNLCGE *QFHRTQFCAFAFFV**SNYYSCTRGI*YSPFF IYSKPLGQSF*L*LLPGLVGRVAEKEENCHGKS* MSKTILPERNTHPGCGHSGLHL**RK**Q*LLP TFSLSY*MYLLGYSRPM*QQGFEGLAERYSKRC HRVVSGWKPIYTGSQGTLQLQTFNTYRLK*QQNK HAF*SELQQHDPAPHLNS*LQPSEMYSSSHL*W IKVSSITFSTWK*HFCCA*RCFQ*SFCIITSSN WSQPSLL*L*HAVVIRLGEVGI*GAWNCSLCWSW RNGR*TFTHNSLQKIYLSRSCGCQYSS*V*PLP IKSV*K*WHM***SS*LLPMHLSIWFQGAGL*C PNSCLHQ*PM*TWRNLPLKGRRRRWILVYLC*WI *RRKL*SQR**L*R**L*K*FYMCRWH**LHMP LPT*VYR*VV*GEAGLLCPGPEPLPARFKVHPN SKGIQM*LHTRVRR*TLRHRF*RLPRQQV*KRSP LHRCSERLYVHMPRRLQWLVL*VFSTHGPPSYQ PL**F*LSEWSSVYRQNK*ANMSVFAWLSGRKV *KIG*CEFYKQRVLSSDSFSQGSASDEHNTSDCH R*RQRNPPV*G*QRPYRGRTLSGACSCQL*HRL SSSFCHLQCGDNQ*WKLPHCGTTCLGSESLFVR GWWEPQNHH*LVKAVHSEF*LSTLCRRHAREE*R GISAPGPWAERNQLPRLHPEPLHQQ*AAGLPEG ADANRHFAWL*AMPQEGVCPWHMPAQQPGRLHL RVPGRMDGAPL*PTDQ*PLPWK*MRTWHLLAHQC VLLQL*VLGGPWRCPL**RGGSV*PMPGDQVQA WEVQAFRSGAALL*MQQWIHGGQL*SRNLLSRG KDKRLLPKAAGLCCLPNNQEGVPIRVQRWVCRRA VLWTAEEQAAEILFRMH*RLLLCGRG*ESGEVR LYEVCVLNTLPAALSLEKVVYFLTVWD**MLHS GNI*NIL*NTEQTYFYYENKDFFSAFGKKKKEML ELKLPLCWRSMKKDIPGDIRTAMGTIATRVHLC NMLKTSRSTCTRDRGATVHCCEIAQSIKPVYPP SHQPSSLGHTKHMRVNEDARQENGTPEFTDRTNG *EIFHAKYKVS*GSGFHLREEMKVLK*ILSSF* MSACQQKQHTKVFTIFQY*FFCNINDKGDDKEP IDY**IN***

Frame 1

APRALLRARWASAPSSFRSPL*SGP*YLLRSPL LSCTFATGIGFAEACVGSEDRPPHNNRPLHLSS RWKPQLFYRQVHPWETEDA*SPLRCSWGLLAALA RRIHPQDLKLPKELLLCQRRVERAVGGVCLSGL YCLVPYYLVHIFPGTLGC*PRRALGLRHCAVPS EQQAKESPQCRRGRRRRGKMRGVGWQMLSLSLGL VLAILNKVAPQACPAQCSCSGSTVDCHGLALRS VPRNIPRNTERLDLNGNNITRITKTDFAGLRHL RVLQLMENKISTIERGAFQDLKELERLRLNRNHL QLFPELLFLGTAKLYRLDLSENQIQAIPRKAFR GAVDIKNLQLDYNQISCIEDGAFRALRDLEVLT LNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDC HLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVA EVQKREFVCSGHQSFMAPSCSVLHCPAACTCSN NIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVI PPGAFSPYKKLRRIDLSNNQISELAPDAFQGLR SLNSLVLYGNKITELPKSLFEGLFSLQLLLLNA NKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGT FSPLRAIQTMHLAQNPFICDCHLKWLADYLHTN PIETSGARCTSPRRLANKRIGQIKSKKFRCSGT EDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQK LNKIPEHIPQYTAELRLNNNEFTVLEATGIFKK LPQLRKINFSNNKITDIEEGAFEGASGVNEILL TSNRLENVQHKMFKGLESLKTLMLRSNRITCVGN DSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSL STLNLLANPFNCNCYLAWLGEWLRKKRIVTGNP RCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSP LSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDV TELYLDGNQFTLVPKELSNYKHLTLIDLSNNRI STLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDG LKSLRLLSLHGNDISVVPEGAFNDLSALSHLAI GANPLYCDCNMQWLSDWVKSEYKEPGIARCAGP GEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCL SNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDV PIHACISNPCKHGGTCHLKEGEEDGFWCICADG FEGENCEVNVDDCEDNDCENNSTCVDGINNYTCL CPPEYTGELCEEKLDFCAQDLNPCQHDSKCILT PKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGA HCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTS PCDNFDCQNGAQCIVRINEPICQCLPGYQGEKC EKLVSVNFINKESYLQIPSAKVRPQTNITLQIA TDEDSGILLYKGDKDHIAVELYRGRVRASYDTGS HPASAIYSVETINDGNFHIVELLALDQSLSLSV DGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSN VASLRQAPGQNGTSFHGCIRNLYINSELQDFQKV PMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTC ECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPIN AFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKH GKCRLSGLGQPYCECSSGYTGDSCDREISCRGE RIRDYYQKQQGYAACQTTKKVSRLECRGGCAGG QCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCG CTRCVS*THSRQLCLWKRLYTS*PCGTNECFIV EIFEIYCKIQNRLIFIMRIKTFFLHLEKKKKKC LN*SFPYAGEV*RKIYLETLEQRWEPLQLGSIFV TC*RQAEAHARGTEELLCTAVKLPRA*NLCTLL HINQVH*DIPSTCV*MRMQGKRMELQNSQIEQM DEKYFMQNIKCPEDLGSIYERK*KC*NKFYLPFK CQHVSRSSTQKSLPFSSINFFVI*MIKEMIKNQ *IIDK*ISNN

Frame 2

PRALCYGPAGLPRLLASGAHFDRGHNTY*DPLF CLVPSPLASDLQKRAWDQRTALPTTTGPCILAA VGSPSSFTAKFILGRQKTRDLLSAALGVSLQPW PGGFILRT*SCPRSSCSARGGWRGRWEACA*VGS TALFHIIWCTFSLALWVASPAGHWASDTARFPR SSKLKKAPSAGEEGGGGERCAALAGRCCPCRWG *CWRS*TRWHRRRARRSALARAAQWTVTGWRCAA CPGISPATPRDWI*MEITSQELRRQILLVLDI* EFFSLWRIRLAPLKEEHSRILKN*RDCV*TEIT FSCFLSCCFLGLRSYTGLISVKTKFRQSQGKLSV GQLT*KICNWITTRSAVLKMGHSGLSGTWKCSL STITTLLDFLWQVSTICLNLGLFDCIQTTCIVT ATWPGSPTGFAKGLGLVCTLSVWAPPT*EAIM*P RFKNENLSAVVTSHLWLLLVVFCTALPPVPVAT IS*TVVGKVSLRSPQIFQRPSQKYVWNRTQSKS SLLELSHHIKSLDELT*AIIRSLNLHQMLSKDYA L*IHLSSMEIKSQNSPKVYLKDCFPYSSYY*MP TR*TAFG*MLFRISTT*TFSPYMTTSFRPSPRG PFHLFGPFKLCIWPRTPLFVTAISSG*RIISIPT RLRPVVPVAPAPAAWQTKELDRSKARNSVVQVQ KIIDQN*VETALRIWLALKSVAVKEPQ*IALIK SSTKSRSTFPSTLQSCVSIIMNLPCWKPQESLRN FLNYVK*TLATIRSQILRREHLKEHLV*MKYFL RVIVWKMCSIRCSRDWKASKL*C*EAIE*PVWG MTVS*DSVLCVCFLCMIIKLLQLHQGHLILSILY LL*TSWPILLTVTATWLGWESG*ERRELSREIL DVKNHTS*KKYPSRMWPFRTSLVMTEMMTIVAP HFLAVLLNVLAWIQSSDVATRV*RSCRKVFQEMS QSCIWMETNLHWFPRNSPTTNI*HL*T*VTTE* ARFLIRASAT*PSSSP*FLVTTV*DVFLLAPLM D*SLFDYFLYMEMTFLLCLKVLSMIFLHYHI*QL EPTLFTVIVTCSGYPTG*SRNIRSLELLVVLVL EKWQINFYSQLPPKNLPVKVLWMSIF*LSVTPA YQIRVKMMAHVIVIQLTFTDAPVHMVSRGRTVMS QFMPASVTHVNMEELAT*RKEKKMDSGVFVLMD LKEKIVKSTLMIVKIMTVKIILHVSMALITTHA FAHLSIQVSCVRRSWTSVPRT*TPASTIQSAS*L QRDSNVTAHQGT*VNTATSILTTAKTTSVKTEP TAQMQ*TAIRAYAPKVTVACSVSFLHPWSSLVP APVIILIVRMELSVSSE*MSQYVSVCLAIREKSV KNWLV*IL*TKSLIFRFLQPRFGLRRT*HFRLP QMKTAESSCIRVTKTISR*NSIGGVFVPAMTPA LIQLLPFTVWRQSMMETSTLWNYLPWIRVSLCPW MVGTPKSSLTCQSSPL*ILTLHSM*EACQGRVT WHLCARPLGRTEPASTAASGTFTSTVSCRTSRR CRCKQAFCLAVSHATRRCVPMAHASPAARQASPA SARKDGWGPSVTNGPMTLALEINAYMAPACPSM RSPTAVSAWRAMEVSSVMKRRICLTHARRSSAS MGSAGFQVWGSPTVNAAVDTRGTAVIEKSLVEGK G*EIITKSSRAMLLAKQPRRCPD*SAEVGVQEG SAVDR*GASGGNTLSNALTAPPLWTRLRKW*SA AVRGVCPKHTPGSSVFGKGCILLDRVGLMNAS*W KYLKYIVKYRTDLFLL*E*RLFFCIWKKKKRNA *TKASPMLEKYEERYTWRH*NSDGNHCNSGPSL *HAEDKQKHMHEGQRSYCALL*NCPEHKTCVPSF TSTKFTRTYQAHACE*GCKAREWNSRIHR*NKW MRNISCKI*SVLRIWVPFTRGNESAKINFIFLL NVSMSAEAAHKSLYHFPVLIFL*YK**RR**RTN RLLINKLVII

Sequence SLIT2.aAug05

TGCCCCGCGCGCTCTGCTACGGGCCCGCTGGGCTTCCGCGCCTTCTAGCTTCCGGAGCCCACTTTGATCGGGGCCATAATACCTATTGAGATCCCCTCTT CTGTCTTGTACCTTCGCCACTGGCATCGGATTTGCAGAAGCGTGCGTGGGATCAGAGGACCGCCCTCCCCACAACAACCGGCCCCTGCATCTTAGCAGCC GTTGGAAGCCCCAGCTCTTTTACCGCCAAGTTCATCCTTGGGAGACAGAAGACGCGTGATCTCCTCTCCGCTGCTCTTGGGGTCTCCTTGCAGCCCTGGC CAGGCGGATTCATCCTCAGGACCTAAAGTTGCCCAAGGAGCTCCTGCTCTGCCAGAGGAGGGTGGAGAGGGCGGTGGGAGGCGTGTGCCTGAGTGGGCTC TACTGCCTTGTTCCATATTATTTGGTGCACATTTTCCCTGGCACTCTGGGTTGCTAGCCCCGCCGGGCACTGGGCCTCAGACACTGCGCGGTTCCCTCGG AGCAGCAAGCTAAAGAAAGCCCCCAGTGCCGGCGAGGAAGGAGGCGGCGGGGAAAGATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTT AGTGCTGGCGATCCTGAACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCGCAGC GTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATTACGAAGACAGATTTTGCTGGTCTTAGACATCTAA GAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCATTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCT TCAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAAATTCAGGCAATCCCAAGGAAAGCTTTCCGT GGGGCAGTTGACATAAAAAATTTGCAACTGGATTACAACCAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTCACTC TCAACAATAACAACATTACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCATTCAAACAACCTGTATTGTGACTG CCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACACTCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCC GAGGTTCAAAAACGAGAATTTGTCTGCAGTGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCCTGTACCTGTAGCAACA ATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCAGAGACCATCACAGAAATACGTTTGGAACAGAACACAATCAAAGTCAT CCCTCCTGGAGCTTTCTCACCATATAAAAAGCTTAGACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCACCAGATGCTTTCCAAGGACTACGC TCTCTGAATTCACTTGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTCCTTACAGCTCCTATTATTGAATGCCA ACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGATCTCCACAACTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAAGGGGAC CTTTTCACCTCTTCGGGCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCATCTCAAGTGGCTAGCGGATTATCTCCATACCAAC CCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGCTAAAG AACAGTATTTCATTCCAGGTACAGAAGATTATCGATCAAAATTAAGTGGAGACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGAAC CACAGTAGATTGCTCTAATCAAAAGCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAGTTGCGTCTCAATAATAATGAATTTACCGTGTTG GAAGCCACAGGAATCTTTAAGAAACTTCCTCAATTACGTAAAATAAACTTTAGCAACAATAAGATCACAGATATTGAGGAGGGAGCATTTGAAGGAGCAT CTGGTGTAAATGAAATACTTCTTACGAGTAATCGTTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTTGAGAAG CAATCGAATAACCTGTGTGGGGAATGACAGTTTCATAGGACTCAGTTCTGTGCGTTTGCTTTCTTTGTATGATAATCAAATTACTACAGTTGCACCAGGG GCATTTGATACTCTCCATTCTTTATCTACTCTAAACCTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTGGGAGAGTGGCTGAGAAAGA AGAGAATTGTCACGGGAAATCCTAGATGTCAAAAACCATACTTCCTGAAAGAAATACCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGATGACGG AAATGATGACAATAGTTGCTCCCCACTTTCTCGCTGTCCTACTGAATGTACTTGCTTGGATACAGTCGTCCGATGTAGCAACAAGGGTTTGAAGGTCTTG CCGAAAGGTATTCCAAGAGATGTCACAGAGTTGTATCTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACTCTCCAACTACAAACATTTAACACTTA TAGACTTAAGTAACAACAGAATAAGCACGCTTTCTAATCAGAGCTTCAGCAACATGACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCTGAGATG TATTCCTCCTCGCACCTTTGATGGATTAAAGTCTCTTCGATTACTTTCTCTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTT TCTGCATTATCACATCTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAGCCTG GAATTGCTCGTTGTGCTGGTCCTGGAGAAATGGCAGATAAACTTTTACTCACAACTCCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGTCAATAT TCTAGCTAAGTGTAACCCCTGCCTATCAAATCCGTGTAAAAATGATGGCACATGTAATAGTGATCCAGTTGACTTTTACCGATGCACCTGTCCATATGGT TTCAAGGGGCAGGACTGTGATGTCCCAATTCATGCCTGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCACTTAAAGGAAGGAGAAGAAGATGGAT TCTGGTGTATTTGTGCTGATGGATTTGAAGGAGAAAATTGTGAAGTCAACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTACATGTGTCGA TGGCATTAATAACTACACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGAGAAGCTGGACTTCTGTGCCCAGGACCTGAACCCCTGCCAG CACGATTCAAAGTGCATCCTAACTCCAAAGGGATTCAAATGTGACTGCACACCAGGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGCCAAG ACAACAAGTGTAAAAACGGAGCCCACTGCACAGATGCAGTGAACGGCTATACGTGCATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAGTTTTCTCC ACCCATGGTCCTCCCTCGTACCAGCCCCTGTGATAATTTTGATTGTCAGAATGGAGCTCAGTGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTG CCTGGCTATCAGGGAGAAAAGTGTGAAAAATTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTCAGCCAAGGTTCGGCCTCAGA CGAACATAACACTTCAGATTGCCACAGATGAAGACAGCGGAATCCTCCTGTATAAGGGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGGGCGTGT TCGTGCCAGCTATGACACCGGCTCTCATCCAGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAACTACTTGCCTTG GATCAGAGTCTCTCTTTGTCCGTGGATGGTGGGAACCCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCTGAATTTTGACTCTCCACTCTATGTAG GAGGCATGCCAGGGAAGAGTAACGTGGCATCTCTGCGCCAGGCCCCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAG TGAGCTGCAGGACTTCCAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAGAAGGTGTGTGCCCATGGCACATGCCAGCCC AGCAGCCAGGCAGGCTTCACCTGCGAGTGCCAGGAAGGATGGATGGGGCCCCTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGCGTAC ATGGCACCTGCTTGCCCATCAATGCGTTCTCCTACAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAAGAGGAGGATCTGTTTAACCC ATGCCAGGCGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCAGGTCTGGGGCAGCCCTACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGAT CGAGAAATCTCTTGTCGAGGGGAAAGGATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCCCGATTAGAGT GCAGAGGTGGGTGTGCAGGAGGGCAGTGCTGTGGACCGCTGAGGAGCAAGCGGCGGAAATACTCTTTCGAATGCACTGACGGCTCCTCCTTTGTGGACGA GGTTGAGAAAGTGGTGAAGTGCGGCTGTACGAGGTGTGTGTCCTAAACACACTCCCGGCAGCTCTGTCTTTGGAAAAGGTTGTATACTTCTTGACCGTGT GGGACTAATGAATGCTTCATAGTGGAAATATTTGAAATATATTGTAAAATACAGAACAGACTTATTTTTATTATGAGAATAAAGACTTTTTTTCTGCATT TGGAAAAAAAAAAAAAGAAATGCTTGAACTAAAGCTTCCCCTATGCTGGAGAAGTATGAAGAAAGATATACCTGGAGACATTAGAACAGCGATGGGAACC ATTGCAACTCGGGTCCATCTTTGTAACATGCTGAAGACAAGCAGAAGCACATGCACGAGGGACAGAGGAGCTACTGTGCACTGCTGTGAAATTGCCCAGA GCATAAAACCTGTGTACCCTCCTTCACATCAACCAAGTTCACTAGGACATACCAAGCACATGCGTGTGAATGAGGATGCAAGGCAAGAGAATGGAACTCC AGAATTCACAGATAGAACAAATGGATGAGAAATATTTCATGCAAAATATAAAGTGTCCTGAGGATCTGGGTTCCATTTACGAGAGGAAATGAAAGTGCTA AAATAAATTTTATCTTCCTTTTAAATGTCAGCATGTCAGCAGAAGCAGCACACAAAAGTCTTTACCATTTTCCAGTATTAATTTTTTTGTAATATAAATG ATAAAGGAGATGATAAAGAACCAATAGATTATTGATAAATAAATTAGTAATAATA

SLIT2.F36 (forward primer)
SLIT2.R4 (reverse primer)
Single exon skipping

Frame 0

CPARSATGPLGFRAF*LPEPTLIGAIIPIEIPS SVLYLRHWHRICRSVRGIRGPPSPQQPAPAS*QP LEAPALLPPSSSLGDRRRVISSPLLLGSPCSPG QADSSSGPKVAQGAPALPEEGGEGGGRRVPEWA LLPCSILFGAHFPWHSGLLAPPGTGPQTLRGSLG AAS*RKPPVPARKEAAGKDARRWLADAVPVAGV SAGDPEQGGTAGVPGAVLLLGQHSGLSRAGAAQ RAQEYPPQHRETGFKWK*HHKNYEDRFCWS*TSK SSSAYGE*D*HH*KRSIPGS*RTRETAFKQKSP SAVS*VAVSWDCEAIQA*SQ*KPNSGNPKESFP WGS*HKKFATGLQPDQLY*RWGIQGSPGPGSAHS QQ*QHY*TFCGKFQPYA*T*DFSTAFKQPVL*L PPGLALRLASPKASGWSVHSVYGPLPPERP*CS RGSKTRICLQWSPVIYGSFL*CFALPCRLYL*QQ YRRLSWERSH*DPHKSSRDHHRNTFGTEHNQSH PSWSFLTI*KA*TN*PEQ*SDL*TCTRCFPRTT LSEFTCPLWK*NHRTPQKFI*RTVFLTAPIIECQ QDKLPSGRCFSGSPQLEPSLPI*QQASDHRQGD LFTSSGHSNYAFGPEPLYL*LPSQVASGLSPYQ PD*DQWCPLHQPPPPGKQKNWTDQKQEIPLFS*R TVFHSRYRRLSIKIKWRLLCGSGLP*KVSL*RN HSRLL*SKAQQNPGAHSPVHCRVASQ***IYRV GSHRNL*ETSSIT*NKL*QQ*DHRY*GGSI*RSI WCK*NTSYE*SFGKCAA*DVQGIGKPQNFDVEK QSNNLCGE*QFHRTQFCAFAFFV**SNYYSCTR GI*YSPFFIYSKPLGQSF*L*LLPGLVGRVAEKE ENCHGKS*MSKTILPERNTHPGCGHSGLHL**R K**Q*LLPTFSLSY*MYLLGYSRPM*QQGFEGL AERYSKRCHRVVSGWKPIYTGSQGTLQLQTFNTY RLK*QQNKHAF*SELQQHDPAPHLNS*LQPSEM YSSSHL*WIKVSSITFSTWK*HFCCA*RCFQ*S FCIITSSNWSQPSLL*L*HAVVIRLGEVGI*GAW NCSLCWSWRNGR*TFTHNSLQKIYLSRSCGCQY SS*V*PLPIKSV*K*WHM***SS*LLPMHLSIW FQGAGL*CPNSCLHQ*PM*TWRNLPLKGRRRRWI LVYLC*WI*RRKL*SQR**L*R**L*K*FYMCR WH**LHMPLPT*VYR*VV*GEAGLLCPGPEPLP ARFKVHPNSKGIQM*LHTRVRR*TLRHRF*RLPR QQV*KRSPLHRCSERLYVHMPRRLQWLVL*VFS THGPPSYQPL**F*LSEWSSVYRQNK*ANMSVF AWLSGRKV*KIG*CEFYKQRVLSSDSFSQGSASD EHNTSDCHR*RQRNPPV*G*QRPYRGRTLSGAC SCQL*HRLSSSFCHLQCGDNQ*WKLPHCGTTCL GSESLFVRGWWEPQNHH*LVKAVHSEF*LSTLCR RHAREE*RGISAPGPWAERNQLPRLHPEPLHQQ *AAGLPEGADANRHFAWL*AMPQEGVCPWHMPA QQPGRLHLRVPGRMDGAPL*PTDQ*PLPWK*MRT WHLLAHQCVLLQL*VLGGPWRCPL**RGGSV*P MPGDQVQAWEVQAFRSGAALL*MQQWIHGGQL* SRNLLSRGKDKRLLPKAAGLCCLPNNQEGVPIRV QRWVCRRAVLWTAEEQAAEILFRMH*RLLLCGR G*ESGEVRLYEVCVLNTLPAALSLEKVVYFLTV WD**MLHSGNI*NIL*NTEQTYFYYENKDFFSAF GKKKKEMLELKLPLCWRSMKKDIPGDIRTAMGT IATRVHLCNMLKTSRSTCTRDRGATVHCCEIAQ SIKPVYPPSHQPSSLGHTKHMRVNEDARQENGTP EFTDRTNG*EIFHAKYKVS*GSGFHLREEMKVL K*ILSSF*MSACQQKQHTKVFTIFQY*FFCNIN DKGDDKEPIDY**IN***

Frame 1

APRALLRARWASAPSSFRSPL*SGP*YLLRSPL LSCTFATGIGFAEACVGSEDRPPHNNRPLHLSS RWKPQLFYRQVHPWETEDA*SPLRCSWGLLAALA RRIHPQDLKLPKELLLCQRRVERAVGGVCLSGL YCLVPYYLVHIFPGTLGC*PRRALGLRHCAVPS EQQAKESPQCRRGRRRRGKMRGVGWQMLSLSLGL VLAILNKVAPQACPAQCSCSGSTVDCHGLALRS VPRNIPRNTERLDLNGNNITRITKTDFAGLRHL RVLQLMENKISTIERGAFQDLKELERLRLNRNHL QLFPELLFLGTAKLYRLDLSENQIQAIPRKAFR GAVDIKNLQLDYNQISCIEDGAFRALRDLEVLT LNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDC HLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVA EVQKREFVCSGHQSFMAPSCSVLHCPAACTCSN NIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVI PPGAFSPYKKLRRIDLSNNQISELAPDAFQGLR SLNSLVLYGNKITELPKSLFEGLFSLQLLLLNA NKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGT FSPLRAIQTMHLAQNPFICDCHLKWLADYLHTN PIETSGARCTSPRRLANKRIGQIKSKKFRCSAK EQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGT TVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVL EATGIFKKLPQLRKINFSNNKITDIEEGAFEGA SGVNEILLTSNRLENVQHKMFKGLESLKTLMLRS NRITCVGNDSFIGLSSVRLLSLYDNQITTVAPG AFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRK KRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDG NDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVL PKGIPRDVTELYLDGNQFTLVPKELSNYKHLTL IDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRC IPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDL SALSHLAIGANPLYCDCNMQWLSDWVKSEYKEP GIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNI LAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYG FKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG FWCICADGFEGENCEVNVDDCEDNDCENNSTCVD GINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQ HDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQ DNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP PMVLPRTSPCDNFDCQNGAQCIVRINEPICQCL PGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQ TNITLQIATDEDSGILLYKGDKDHIAVELYRGRV RASYDTGSHPASAIYSVETINDGNFHIVELLAL DQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYV GGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINS ELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQP SSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCV HGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNP CQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCD REISCRGERIRDYYQKQQGYAACQTTKKVSRLE CRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDE VEKVVKCGCTRCVS*THSRQLCLWKRLYTS*PC GTNECFIVEIFEIYCKIQNRLIFIMRIKTFFLH LEKKKKKCLN*SFPYAGEV*RKIYLETLEQRWEP LQLGSIFVTC*RQAEAHARGTEELLCTAVKLPR A*NLCTLLHINQVH*DIPSTCV*MRMQGKRMEL QNSQIEQMDEKYFMQNIKCPEDLGSIYERK*KC* NKFYLPFKCQHVSRSSTQKSLPFSSINFFVI*M IKEMIKNQ*IIDK*ISNN

Frame 2

PRALCYGPAGLPRLLASGAHFDRGHNTY*DPLF CLVPSPLASDLQKRAWDQRTALPTTTGPCILAA VGSPSSFTAKFILGRQKTRDLLSAALGVSLQPW PGGFILRT*SCPRSSCSARGGWRGRWEACA*VGS TALFHIIWCTFSLALWVASPAGHWASDTARFPR SSKLKKAPSAGEEGGGGERCAALAGRCCPCRWG *CWRS*TRWHRRRARRSALARAAQWTVTGWRCAA CPGISPATPRDWI*MEITSQELRRQILLVLDI* EFFSLWRIRLAPLKEEHSRILKN*RDCV*TEIT FSCFLSCCFLGLRSYTGLISVKTKFRQSQGKLSV GQLT*KICNWITTRSAVLKMGHSGLSGTWKCSL STITTLLDFLWQVSTICLNLGLFDCIQTTCIVT ATWPGSPTGFAKGLGLVCTLSVWAPPT*EAIM*P RFKNENLSAVVTSHLWLLLVVFCTALPPVPVAT IS*TVVGKVSLRSPQIFQRPSQKYVWNRTQSKS SLLELSHHIKSLDELT*AIIRSLNLHQMLSKDYA L*IHLSSMEIKSQNSPKVYLKDCFPYSSYY*MP TR*TAFG*MLFRISTT*TFSPYMTTSFRPSPRG PFHLFGPFKLCIWPRTPLFVTAISSG*RIISIPT RLRPVVPVAPAPAAWQTKELDRSKARNSVVQLK NSISFQVQKIIDQN*VETALRIWLALKSVAVKE PQ*IALIKSSTKSRSTFPSTLQSCVSIIMNLPCW KPQESLRNFLNYVK*TLATIRSQILRREHLKEH LV*MKYFLRVIVWKMCSIRCSRDWKASKL*C*E AIE*PVWGMTVS*DSVLCVCFLCMIIKLLQLHQG HLILSILYLL*TSWPILLTVTATWLGWESG*ER RELSREILDVKNHTS*KKYPSRMWPFRTSLVMT EMMTIVAPHFLAVLLNVLAWIQSSDVATRV*RSC RKVFQEMSQSCIWMETNLHWFPRNSPTTNI*HL *T*VTTE*ARFLIRASAT*PSSSP*FLVTTV*D VFLLAPLMD*SLFDYFLYMEMTFLLCLKVLSMIF LHYHI*QLEPTLFTVIVTCSGYPTG*SRNIRSL ELLVVLVLEKWQINFYSQLPPKNLPVKVLWMSI F*LSVTPAYQIRVKMMAHVIVIQLTFTDAPVHMV SRGRTVMSQFMPASVTHVNMEELAT*RKEKKMD SGVFVLMDLKEKIVKSTLMIVKIMTVKIILHVS MALITTHAFAHLSIQVSCVRRSWTSVPRT*TPAS TIQSAS*LQRDSNVTAHQGT*VNTATSILTTAK TTSVKTEPTAQMQ*TAIRAYAPKVTVACSVSFL HPWSSLVPAPVIILIVRMELSVSSE*MSQYVSVC LAIREKSVKNWLV*IL*TKSLIFRFLQPRFGLR RT*HFRLPQMKTAESSCIRVTKTISR*NSIGGV FVPAMTPALIQLLPFTVWRQSMMETSTLWNYLPW IRVSLCPWMVGTPKSSLTCQSSPL*ILTLHSM* EACQGRVTWHLCARPLGRTEPASTAASGTFTST VSCRTSRRCRCKQAFCLAVSHATRRCVPMAHASP AARQASPASARKDGWGPSVTNGPMTLALEINAY MAPACPSMRSPTAVSAWRAMEVSSVMKRRICLT HARRSSASMGSAGFQVWGSPTVNAAVDTRGTAVI EKSLVEGKG*EIITKSSRAMLLAKQPRRCPD*S AEVGVQEGSAVDR*GASGGNTLSNALTAPPLWT RLRKW*SAAVRGVCPKHTPGSSVFGKGCILLDRV GLMNAS*WKYLKYIVKYRTDLFLL*E*RLFFCI WKKKKRNA*TKASPMLEKYEERYTWRH*NSDGN HCNSGPSL*HAEDKQKHMHEGQRSYCALL*NCPE HKTCVPSFTSTKFTRTYQAHACE*GCKAREWNS RIHR*NKWMRNISCKI*SVLRIWVPFTRGNESA KINFIFLLNVSMSAEAAHKSLYHFPVLIFL*YK* *RR**RTNRLLINKLVII

Sequence SLIT2.cAug05

TGCCCCGCGCGCTCTGCTACGGGCCCGCTGGGCTTCCGCGCCTTCTAGCTTCCGGAGCCCACTTTGATCGGGGCCATAATACCTATTGAGATCCCCTCTT CTGTCTTGTACCTTCGCCACTGGCATCGGATTTGCAGAAGCGTGCGTGGGATCAGAGGACCGCCCTCCCCACAACAACCGGCCCCTGCATCTTAGCAGCC GTTGGAAGCCCCAGCTCTTTTACCGCCAAGTTCATCCTTGGGAGACAGAAGACGCGTGATCTCCTCTCCGCTGCTCTTGGGGTCTCCTTGCAGCCCTGGC CAGGCGGATTCATCCTCAGGACCTAAAGTTGCCCAAGGAGCTCCTGCTCTGCCAGAGGAGGGTGGAGAGGGCGGTGGGAGGCGTGTGCCTGAGTGGGCTC TACTGCCTTGTTCCATATTATTTGGTGCACATTTTCCCTGGCACTCTGGGTTGCTAGCCCCGCCGGGCACTGGGCCTCAGACACTGCGCGGTTCCCTCGG AGCAGCAAGCTAAAGAAAGCCCCCAGTGCCGGCGAGGAAGGAGGCGGCGGGGAAAGATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTT AGTGCTGGCGATCCTGAACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCGCAGC GTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATTACGAAGACAGATTTTGCTGGTCTTAGACATCTAA GAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCATTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCT TCAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAAATTCAGGCAATCCCAAGGAAAGCTTTCCGT GGGGCAGTTGACATAAAAAATTTGCAACTGGATTACAACCAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTCACTC TCAACAATAACAACATTACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCATTCAAACAACCTGTATTGTGACTG CCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACACTCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCC GAGGTTCAAAAACGAGAATTTGTCTGCAGTGATGAGGAAGAAGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCCTGTA CCTGTAGCAACAATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCAGAGACCATCACAGAAATACGTTTGGAACAGAACAC AATCAAAGTCATCCCTCCTGGAGCTTTCTCACCATATAAAAAGCTTAGACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCACCAGATGCTTTC CAAGGACTACGCTCTCTGAATTCACTTGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTCCTTACAGCTCCTAT TATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGATCTCCACAACTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCAT CGCCAAGGGGACCTTTTCACCTCTTCGGGCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCATCTCAAGTGGCTAGCGGATTAT CTCCATACCAACCCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTT GTTCAGGTACAGAAGATTATCGATCAAAATTAAGTGGAGACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGAACCACAGTAGATTG CTCTAATCAAAAGCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAGTTGCGTCTCAATAATAATGAATTTACCGTGTTGGAAGCCACAGGA ATCTTTAAGAAACTTCCTCAATTACGTAAAATAAACTTTAGCAACAATAAGATCACAGATATTGAGGAGGGAGCATTTGAAGGAGCATCTGGTGTAAATG AAATACTTCTTACGAGTAATCGTTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTTGAGAAGCAATCGAATAAC CTGTGTGGGGAATGACAGTTTCATAGGACTCAGTTCTGTGCGTTTGCTTTCTTTGTATGATAATCAAATTACTACAGTTGCACCAGGGGCATTTGATACT CTCCATTCTTTATCTACTCTAAACCTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTGGGAGAGTGGCTGAGAAAGAAGAGAATTGTCA CGGGAAATCCTAGATGTCAAAAACCATACTTCCTGAAAGAAATACCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGATGACGGAAATGATGACAA TAGTTGCTCCCCACTTTCTCGCTGTCCTACTGAATGTACTTGCTTGGATACAGTCGTCCGATGTAGCAACAAGGGTTTGAAGGTCTTGCCGAAAGGTATT CCAAGAGATGTCACAGAGTTGTATCTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACTCTCCAACTACAAACATTTAACACTTATAGACTTAAGTA ACAACAGAATAAGCACGCTTTCTAATCAGAGCTTCAGCAACATGACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCTGAGATGTATTCCTCCTCG CACCTTTGATGGATTAAAGTCTCTTCGATTACTTTCTCTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTTTCTGCATTATCA CATCTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAGCCTGGAATTGCTCGTT GTGCTGGTCCTGGAGAAATGGCAGATAAACTTTTACTCACAACTCCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGTCAATATTCTAGCTAAGTG TAACCCCTGCCTATCAAATCCGTGTAAAAATGATGGCACATGTAATAGTGATCCAGTTGACTTTTACCGATGCACCTGTCCATATGGTTTCAAGGGGCAG GACTGTGATGTCCCAATTCATGCCTGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCACTTAAAGGAAGGAGAAGAAGATGGATTCTGGTGTATTT GTGCTGATGGATTTGAAGGAGAAAATTGTGAAGTCAACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTACATGTGTCGATGGCATTAATAA CTACACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGAGAAGCTGGACTTCTGTGCCCAGGACCTGAACCCCTGCCAGCACGATTCAAAG TGCATCCTAACTCCAAAGGGATTCAAATGTGACTGCACACCAGGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGCCAAGACAACAAGTGTA AAAACGGAGCCCACTGCACAGATGCAGTGAACGGCTATACGTGCATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCT CCCTCGTACCAGCCCCTGTGATAATTTTGATTGTCAGAATGGAGCTCAGTGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTGCCTGGCTATCAG GGAGAAAAGTGTGAAAAATTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTCAGCCAAGGTTCGGCCTCAGACGAACATAACAC TTCAGATTGCCACAGATGAAGACAGCGGAATCCTCCTGTATAAGGGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGGGCGTGTTCGTGCCAGCTA TGACACCGGCTCTCATCCAGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAACTACTTGCCTTGGATCAGAGTCTC TCTTTGTCCGTGGATGGTGGGAACCCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCTGAATTTTGACTCTCCACTCTATGTAGGAGGCATGCCAG GGAAGAGTAACGTGGCATCTCTGCGCCAGGCCCCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAGTGAGCTGCAGGA CTTCCAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAGAAGGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAGGCA GGCTTCACCTGCGAGTGCCAGGAAGGATGGATGGGGCCCCTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGCGTACATGGCACCTGCT TGCCCATCAATGCGTTCTCCTACAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAAGAGGAGGATCTGTTTAACCCATGCCAGGCGAT CAAGTGCAAGCATGGGAAGTGCAGGCTTTCAGGTCTGGGGCAGCCCTACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGATCGAGAAATCTCT TGTCGAGGGGAAAGGATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCCCGATTAGAGTGCAGAGGTGGGT GTGCAGGAGGGCAGTGCTGTGGACCGCTGAGGAGCAAGCGGCGGAAATACTCTTTCGAATGCACTGACGGCTCCTCCTTTGTGGACGAGGTTGAGAAAGT GGTGAAGTGCGGCTGTACGAGGTGTGTGTCCTAAACACACTCCCGGCAGCTCTGTCTTTGGAAAAGGTTGTATACTTCTTGACCGTGTGGGACTAATGAA TGCTTCATAGTGGAAATATTTGAAATATATTGTAAAATACAGAACAGACTTATTTTTATTATGAGAATAAAGACTTTTTTTCTGCATTTGGAAAAAAAAA AAAAGAAATGCTTGAACTAAAGCTTCCCCTATGCTGGAGAAGTATGAAGAAAGATATACCTGGAGACATTAGAACAGCGATGGGAACCATTGCAACTCGG GTCCATCTTTGTAACATGCTGAAGACAAGCAGAAGCACATGCACGAGGGACAGAGGAGCTACTGTGCACTGCTGTGAAATTGCCCAGAGCATAAAACCTG TGTACCCTCCTTCACATCAACCAAGTTCACTAGGACATACCAAGCACATGCGTGTGAATGAGGATGCAAGGCAAGAGAATGGAACTCCAGAATTCACAGA TAGAACAAATGGATGAGAAATATTTCATGCAAAATATAAAGTGTCCTGAGGATCTGGGTTCCATTTACGAGAGGAAATGAAAGTGCTAAAATAAATTTTA TCTTCCTTTTAAATGTCAGCATGTCAGCAGAAGCAGCACACAAAAGTCTTTACCATTTTCCAGTATTAATTTTTTTGTAATATAAATGATAAAGGAGATG ATAAAGAACCAATAGATTATTGATAAATAAATTAGTAATAATA

SLIT2.F36 (forward primer)
SLIT2.R4 (reverse primer)
Single exon skipping

Frame 0

CPARSATGPLGFRAF*LPEPTLIGAIIPIEIPS SVLYLRHWHRICRSVRGIRGPPSPQQPAPAS*QP LEAPALLPPSSSLGDRRRVISSPLLLGSPCSPG QADSSSGPKVAQGAPALPEEGGEGGGRRVPEWA LLPCSILFGAHFPWHSGLLAPPGTGPQTLRGSLG AAS*RKPPVPARKEAAGKDARRWLADAVPVAGV SAGDPEQGGTAGVPGAVLLLGQHSGLSRAGAAQ RAQEYPPQHRETGFKWK*HHKNYEDRFCWS*TSK SSSAYGE*D*HH*KRSIPGS*RTRETAFKQKSP SAVS*VAVSWDCEAIQA*SQ*KPNSGNPKESFP WGS*HKKFATGLQPDQLY*RWGIQGSPGPGSAHS QQ*QHY*TFCGKFQPYA*T*DFSTAFKQPVL*L PPGLALRLASPKASGWSVHSVYGPLPPERP*CS RGSKTRICLQ**GRRSPVIYGSFL*CFALPCRLY L*QQYRRLSWERSH*DPHKSSRDHHRNTFGTEH NQSHPSWSFLTI*KA*TN*PEQ*SDL*TCTRCF PRTTLSEFTCPLWK*NHRTPQKFI*RTVFLTAPI IECQQDKLPSGRCFSGSPQLEPSLPI*QQASDH RQGDLFTSSGHSNYAFGPEPLYL*LPSQVASGL SPYQPD*DQWCPLHQPPPPGKQKNWTDQKQEIPL FRYRRLSIKIKWRLLCGSGLP*KVSL*RNHSRL L*SKAQQNPGAHSPVHCRVASQ***IYRVGSHR NL*ETSSIT*NKL*QQ*DHRY*GGSI*RSIWCK* NTSYE*SFGKCAA*DVQGIGKPQNFDVEKQSNN LCGE*QFHRTQFCAFAFFV**SNYYSCTRGI*Y SPFFIYSKPLGQSF*L*LLPGLVGRVAEKEENCH GKS*MSKTILPERNTHPGCGHSGLHL**RK**Q *LLPTFSLSY*MYLLGYSRPM*QQGFEGLAERY SKRCHRVVSGWKPIYTGSQGTLQLQTFNTYRLK* QQNKHAF*SELQQHDPAPHLNS*LQPSEMYSSS HL*WIKVSSITFSTWK*HFCCA*RCFQ*SFCII TSSNWSQPSLL*L*HAVVIRLGEVGI*GAWNCSL CWSWRNGR*TFTHNSLQKIYLSRSCGCQYSS*V *PLPIKSV*K*WHM***SS*LLPMHLSIWFQGA GL*CPNSCLHQ*PM*TWRNLPLKGRRRRWILVYL C*WI*RRKL*SQR**L*R**L*K*FYMCRWH** LHMPLPT*VYR*VV*GEAGLLCPGPEPLPARFK VHPNSKGIQM*LHTRVRR*TLRHRF*RLPRQQV* KRSPLHRCSERLYVHMPRRLQWLVL*VFSTHGP PSYQPL**F*LSEWSSVYRQNK*ANMSVFAWLS GRKV*KIG*CEFYKQRVLSSDSFSQGSASDEHNT SDCHR*RQRNPPV*G*QRPYRGRTLSGACSCQL *HRLSSSFCHLQCGDNQ*WKLPHCGTTCLGSES LFVRGWWEPQNHH*LVKAVHSEF*LSTLCRRHAR EE*RGISAPGPWAERNQLPRLHPEPLHQQ*AAG LPEGADANRHFAWL*AMPQEGVCPWHMPAQQPG RLHLRVPGRMDGAPL*PTDQ*PLPWK*MRTWHLL AHQCVLLQL*VLGGPWRCPL**RGGSV*PMPGD QVQAWEVQAFRSGAALL*MQQWIHGGQL*SRNL LSRGKDKRLLPKAAGLCCLPNNQEGVPIRVQRWV CRRAVLWTAEEQAAEILFRMH*RLLLCGRG*ES GEVRLYEVCVLNTLPAALSLEKVVYFLTVWD** MLHSGNI*NIL*NTEQTYFYYENKDFFSAFGKKK KEMLELKLPLCWRSMKKDIPGDIRTAMGTIATR VHLCNMLKTSRSTCTRDRGATVHCCEIAQSIKP VYPPSHQPSSLGHTKHMRVNEDARQENGTPEFTD RTNG*EIFHAKYKVS*GSGFHLREEMKVLK*IL SSF*MSACQQKQHTKVFTIFQY*FFCNINDKGD DKEPIDY**IN***

Frame 1

APRALLRARWASAPSSFRSPL*SGP*YLLRSPL LSCTFATGIGFAEACVGSEDRPPHNNRPLHLSS RWKPQLFYRQVHPWETEDA*SPLRCSWGLLAALA RRIHPQDLKLPKELLLCQRRVERAVGGVCLSGL YCLVPYYLVHIFPGTLGC*PRRALGLRHCAVPS EQQAKESPQCRRGRRRRGKMRGVGWQMLSLSLGL VLAILNKVAPQACPAQCSCSGSTVDCHGLALRS VPRNIPRNTERLDLNGNNITRITKTDFAGLRHL RVLQLMENKISTIERGAFQDLKELERLRLNRNHL QLFPELLFLGTAKLYRLDLSENQIQAIPRKAFR GAVDIKNLQLDYNQISCIEDGAFRALRDLEVLT LNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDC HLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVA EVQKREFVCSDEEEGHQSFMAPSCSVLHCPAAC TCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNT IKVIPPGAFSPYKKLRRIDLSNNQISELAPDAF QGLRSLNSLVLYGNKITELPKSLFEGLFSLQLL LLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTI AKGTFSPLRAIQTMHLAQNPFICDCHLKWLADY LHTNPIETSGARCTSPRRLANKRIGQIKSKKFR CSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDC SNQKLNKIPEHIPQYTAELRLNNNEFTVLEATG IFKKLPQLRKINFSNNKITDIEEGAFEGASGVN EILLTSNRLENVQHKMFKGLESLKTLMLRSNRIT CVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDT LHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIV TGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDN SCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGI PRDVTELYLDGNQFTLVPKELSNYKHLTLIDLS NNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPR TFDGLKSLRLLSLHGNDISVVPEGAFNDLSALS HLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR CAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKC NPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQ DCDVPIHACISNPCKHGGTCHLKEGEEDGFWCI CADGFEGENCEVNVDDCEDNDCENNSTCVDGINN YTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSK CILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKC KNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVL PRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQ GEKCEKLVSVNFINKESYLQIPSAKVRPQTNIT LQIATDEDSGILLYKGDKDHIAVELYRGRVRASY DTGSHPASAIYSVETINDGNFHIVELLALDQSL SLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMP GKSNVASLRQAPGQNGTSFHGCIRNLYINSELQD FQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQA GFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTC LPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAI KCKHGKCRLSGLGQPYCECSSGYTGDSCDREIS CRGERIRDYYQKQQGYAACQTTKKVSRLECRGG CAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKV VKCGCTRCVS*THSRQLCLWKRLYTS*PCGTNE CFIVEIFEIYCKIQNRLIFIMRIKTFFLHLEKK KKKCLN*SFPYAGEV*RKIYLETLEQRWEPLQLG SIFVTC*RQAEAHARGTEELLCTAVKLPRA*NL CTLLHINQVH*DIPSTCV*MRMQGKRMELQNSQ IEQMDEKYFMQNIKCPEDLGSIYERK*KC*NKFY LPFKCQHVSRSSTQKSLPFSSINFFVI*MIKEM IKNQ*IIDK*ISNN

Frame 2

PRALCYGPAGLPRLLASGAHFDRGHNTY*DPLF CLVPSPLASDLQKRAWDQRTALPTTTGPCILAA VGSPSSFTAKFILGRQKTRDLLSAALGVSLQPW PGGFILRT*SCPRSSCSARGGWRGRWEACA*VGS TALFHIIWCTFSLALWVASPAGHWASDTARFPR SSKLKKAPSAGEEGGGGERCAALAGRCCPCRWG *CWRS*TRWHRRRARRSALARAAQWTVTGWRCAA CPGISPATPRDWI*MEITSQELRRQILLVLDI* EFFSLWRIRLAPLKEEHSRILKN*RDCV*TEIT FSCFLSCCFLGLRSYTGLISVKTKFRQSQGKLSV GQLT*KICNWITTRSAVLKMGHSGLSGTWKCSL STITTLLDFLWQVSTICLNLGLFDCIQTTCIVT ATWPGSPTGFAKGLGLVCTLSVWAPPT*EAIM*P RFKNENLSAVMRKKVTSHLWLLLVVFCTALPPV PVATIS*TVVGKVSLRSPQIFQRPSQKYVWNRT QSKSSLLELSHHIKSLDELT*AIIRSLNLHQMLS KDYAL*IHLSSMEIKSQNSPKVYLKDCFPYSSY Y*MPTR*TAFG*MLFRISTT*TFSPYMTTSFRP SPRGPFHLFGPFKLCIWPRTPLFVTAISSG*RII SIPTRLRPVVPVAPAPAAWQTKELDRSKARNSV VQVQKIIDQN*VETALRIWLALKSVAVKEPQ*I ALIKSSTKSRSTFPSTLQSCVSIIMNLPCWKPQE SLRNFLNYVK*TLATIRSQILRREHLKEHLV*M KYFLRVIVWKMCSIRCSRDWKASKL*C*EAIE* PVWGMTVS*DSVLCVCFLCMIIKLLQLHQGHLIL SILYLL*TSWPILLTVTATWLGWESG*ERRELS REILDVKNHTS*KKYPSRMWPFRTSLVMTEMMT IVAPHFLAVLLNVLAWIQSSDVATRV*RSCRKVF QEMSQSCIWMETNLHWFPRNSPTTNI*HL*T*V TTE*ARFLIRASAT*PSSSP*FLVTTV*DVFLL APLMD*SLFDYFLYMEMTFLLCLKVLSMIFLHYH I*QLEPTLFTVIVTCSGYPTG*SRNIRSLELLV VLVLEKWQINFYSQLPPKNLPVKVLWMSIF*LS VTPAYQIRVKMMAHVIVIQLTFTDAPVHMVSRGR TVMSQFMPASVTHVNMEELAT*RKEKKMDSGVF VLMDLKEKIVKSTLMIVKIMTVKIILHVSMALI TTHAFAHLSIQVSCVRRSWTSVPRT*TPASTIQS AS*LQRDSNVTAHQGT*VNTATSILTTAKTTSV KTEPTAQMQ*TAIRAYAPKVTVACSVSFLHPWS SLVPAPVIILIVRMELSVSSE*MSQYVSVCLAIR EKSVKNWLV*IL*TKSLIFRFLQPRFGLRRT*H FRLPQMKTAESSCIRVTKTISR*NSIGGVFVPA MTPALIQLLPFTVWRQSMMETSTLWNYLPWIRVS LCPWMVGTPKSSLTCQSSPL*ILTLHSM*EACQ GRVTWHLCARPLGRTEPASTAASGTFTSTVSCR TSRRCRCKQAFCLAVSHATRRCVPMAHASPAARQ ASPASARKDGWGPSVTNGPMTLALEINAYMAPA CPSMRSPTAVSAWRAMEVSSVMKRRICLTHARR SSASMGSAGFQVWGSPTVNAAVDTRGTAVIEKSL VEGKG*EIITKSSRAMLLAKQPRRCPD*SAEVG VQEGSAVDR*GASGGNTLSNALTAPPLWTRLRK W*SAAVRGVCPKHTPGSSVFGKGCILLDRVGLMN AS*WKYLKYIVKYRTDLFLL*E*RLFFCIWKKK KRNA*TKASPMLEKYEERYTWRH*NSDGNHCNS GPSL*HAEDKQKHMHEGQRSYCALL*NCPEHKTC VPSFTSTKFTRTYQAHACE*GCKAREWNSRIHR *NKWMRNISCKI*SVLRIWVPFTRGNESAKINF IFLLNVSMSAEAAHKSLYHFPVLIFL*YK**RR* *RTNRLLINKLVII