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Sequences

Sequence RAD52.aAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCA GTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCA TGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCAC GCAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGATGGTTCATATCATGAA GATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCA GGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATTTAAC TAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCAGGTG ACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACC AGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAGCAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGC AGCGCCTCCGGCCCCTCCTGTGACGCACAGCACTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATC AAGACTCTTGAAGACAACTCTGAAAAGTGGGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTG ACACATTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCA AACTTATAGCGCTGACCAACGCACAACAGGAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTC AGGCCACATAATTGGACTCTGTCACAAAGGGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTT ATCTTGCTTCATTCTGAACCTTATCAAGAGGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGG AGAGGCTCTGGACAGATGAATACCTTTTCTTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATC ACCAATGGCTCCTTGCCCTCAAGCAATATAGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCT TCTTTCCTTTACGGTCCATGCCTTTAATGTTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCA ATCCAGGCTACAATCTTCATTTAATATAAATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAA TAATCATCTAAGACCACGAAAAAAGGCTGTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTG GTGCAAACACAGCTCTCTCCACAACCTCTTGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGG CCAGAAAAGGCATTTTTGAGAAAGCAAATCGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTT GCATTTTTTGAATATTTGTATACACATAATGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACAC ATAATTTTGTGCATGAAACAGAGTTTTTGTATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTG TATGCCTGGTGCTTTGCGACTTGTCACACGAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATT TCAATTAGGGATGCTCAAACGCAACTGTTTCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGAT GTTTCATAATTCTTCCCCCAAAATAATGAAGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGG TGCCATCTTGACTCATTGCAGCCTCCGCCTGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGC CCGACTAATTTTTGTATTTTTAGTAGAGGAGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCC CAAAGTAAAAAGTACTAGAATTATAGGCATGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCT TGAAGCAGTGACTTCCTATTTTTTGGCTCAGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCT ATTCCTTTCGAAGTTAAGGAGACAGCAAAGGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAA GGAATCTTCTGGAAATGAAACCCCACAGCTCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCA CAAAGCGCAATCTTACAAGAAGCGCAACACCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTAT TTTTAACAGTGTACATTGTTAAATAATGTAAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAA AATAATTTAAACAGTTCAGTAGCACTAGTTCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTT TTTTTGTGTATGAGTGAAAAGATGTCAAAATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTA TGTTTCACTCTTGGGTGGCCCCTTTCTCAGCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCA TTTTAAAGCAGGGGTGGAAGCCCTTGCCCTTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTAC TTACTAAGGGAAGCAGGGCCCTTAAGGAATGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATG CCATTGACGTGGGTAAAAGGTTGGAAATATTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCA GAGATAACAAAGATCCTAAACATAGCCAGTTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAA TAACACTGATTGGTTGCAGTTATTACTTACGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGA TTATGATCCTGCAACCATTTGGACATCATAAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTA ACTTTCCACAAAATGTAGTACCCCAAGCTGAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCA TCTGTGACTTTTCACTATCAGGTTCCCCAACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTA GCTCAAATAAGAAAGGTTTATATGACAGTCACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTT TAAGTTGTCCAATTTTGTTACAACACCCCCTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTC ATCTTACACCTGCTCTAGACAAAGTTGTTGAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGG ACCTACGATTTCCTTCCACATGCCCTTTATTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGC AAGCAACACTGGGAAACAGAAGTATTCAAGTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTT AAAGTGTCTCAATAAGTAAAGGACAGAGCTAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTA TATATCACAGTGTTTCCACATCAAAACTGATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGC TGCTAATATCTTTTGCTTAAAGCACTAATGACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAA AATAAAAAAGAGAGGGGTAAAAAAAAGGGAGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGG GATTCAGGGCATGTACCAAAAGCAAGAGCTCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTT TCCTAGCCCTCATCCTCATTACTGAGTATAGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTC CAATAACTGGAGCGGGGGAGACTCTCCATTCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGT AGTTAGTATATAGGCTACTTCCCACCCCCACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCC GCAGGTTGGAGCCTGGGGGGTTGCTGATGGATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGG CTGTGGTGCTGGGCCACCCGTCCCACTCCATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCT AGAGAGGTAGCTGATGCTGCAGAGATGGGGGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTC CAGGGCCCTGAAAACAAAACCACCACGTGTCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGAT ACAGCAGAACAGTCTTCTGTAGTATTTATTGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGT GTCTGTGTGTGTGTAGTTGAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWILLTSTMASSTWESVHL*GSS*RMVHIMK MLVMVLVRASSPRLYLWRRQGRRR*QTG*SEPS GVLGMHLETVFWTKTT*DH*ISFHASCLLKWI* LKRRDKILNRLWRRQDTTAADRTWPWDTHSCSR* PPLPDPAML*YRRTRTAAPEA*AHPPWRARPRT SGSSGRSSCSSSSGSGWRSSRFESPRRQLRRVR QRLRPLL*RTALL*LSQNHSWRKTSLQE*LKN*S RLLKTTLKSGL*LPMQGMVWSSPRLEQTQPRPL TH*P*TTRW*PRTGLHTAFATRNHKQNLDLGTS KLIALTNAQQETGNLIGRART*RKGNMIHLN*GS GHIIGLCHKGTLENYFLVMKLFIAAGE*TSLRF ILLHSEPYQEDLTESPLQLELSTFEKLVHHSSR ERLWTDEYLFFGL*GFPLFITELLC**RWTF*ES PMAPCPQAI*ARLGPKHLPQQLSTFSCFA*RLP SFLYGPCL*CCPH*ALWITTGKRLEQCFSLLCI NPGYNLHLI*IIYGFMTLQSCIVSRLTFFPKEGN NHLRPRKKAVFCFFFFFFF*DGVWLCCPDWSSV VQTQLSPQPLGPK*YSHLCLTKYRDYWCEPLCL ARKGIFEKANRIPY*QNRIYIYCLSEMLETRIVL HFLNICIHIMRPWGWDPSLNVEFTCVSCICLIH IILCMKQSFCIRRYTAAEEGWVFFSLRVAA*TV VCLVLCDLSHEVTCGIFHFWHHVSAQKFSDLRAF QLGMLKRNCFYFPISGVRCNPP*P*IGFS*CSD VS*FFPQNNEVCSLTTFFLRGGVWLCSAGWSAA VPS*LIAASACQDSSNSPASGSRVAGITDTHHDA RLIFVFLVEETGFLHVGQAGLKLTSSDLPTSAS QSKKY*NYRHEPLRPANLIIFLDVLFWAGLWLI LKQ*LPIFWLRPIWEAVNLR*GSLKVLAGSRPFL FLSKLRRQQRSSQELGIQL*IALNSK*CQ*ECE GIFWK*NPTAQQKQVWFI*MVSLNKSTKVTN*S TKRNLTRSATPRVSAKCTINGC*ETPKV*IFIFI FNSVHC*IM*GKHL*FRKFLMWKKILKVYY*HK NNLNSSVALVHSSRLVISYTGG*NQQ*ITPVLT FFVYE*KDVKIIIKPK*QMIMCCMRLHCSSGIKY VSLLGGPFLSYSAPCWFYPYSLHKKYKQRINKS F*SRGGSPCPFPFYLLKTFSSHLHI*GRRPTYL LTKGSRALKE*TGYRSLQPLSVVVVVVVVIPRIC H*RG*KVGNIFSFPTKSQEAPQN*ES*LLSLSS EITKILNIAS*DSISGKL*K*LITTHYKEA*H* ITLIGCSYYLRGVLFI*KCEHISCTSRNKGGTID YDPATIWTS*RK*VGISILS*ND*FRCGKIDRL TFHKM*YPKLRNAWYMCNKRKAIQTVFLRAIFS ICDFSLSGSPT*KLAPHPLLFMFSFF*VTSVQA* LK*ERFI*QSLARHHVCFLIL*SLSFTQHTPFF *VVQFCYNTPSSLFPKQNFLFLTTDANLTSHPT HLTPALDKVVE*LKKILFQFPFGITTAGLLGSHG PTISFHMPFIDLGSGKLMLMAEAIEVQGTKMSS KQHWETEVFKYSNLYNFTFTFIYLIQWKYKGKS *SVSISKGQS*LFLELFKRFIFYS*NYCCPTTFY ISQCFHIKTDGKVTCPQGT*FFFFPNC*CCSQS C*YLLLKALMTYHKKELHPSTLVFFTGF*RAKK K*KREG*KKGRRAG*ERAQ*VVFVLWFGFLRE*G IQGMYQKQELHPLEITDGFDFTLSSPASL*TRL S*PSSSLLSIDKQESMV*SCPLTKKLFFPSSPI PITGAGETLHSEGSMQY*KHSPLARNNQLNAALV VSI*ATSHPHPPPQHSISCPQIYSVNVSRSKWS AGWSLGGC*WISANC*C*NSARRQFPQVGTGQV AVVLGHPSHSIERQMG*LESHTAVGGT*TW*SDL ER*LMLQRWGQSHPGSRSWRCTVAQVQRTSLPF QGPENKTTTCQVWCGPWYKMCGANIYF*YNPVR YSRTVFCSIYWLFIFTK*G*K*SS*EGGMSCACV SVCV*LRMTK

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGWFIS* RCWLWC**GPQVQGFIFGEGKEGGGDRRAEASPQ EFWECTWKLYSGQRLPEITK*ASTPVAS*SGFN *SEETRS*TVCGGGKIQQLPTEHGPGTPTAAAG DLPFQTQPCCDTGGPGLQLPKPELIRRGERGHAP AEAPAEAAAAAVPGADGEAAGSSLHAVS*EE*G SASGPSCDAQHSCNCLRTTPGERLPCRSDSRIN QDS*RQL*KVGCDSRCRGWCGQALV*SRPSPDL* HISLEQPDGDPEQDSTQRLPPETTSKIWILGPP NL*R*PTHNRKLGIS*EEPGHEEKEI*SILTEA QAT*LDSVTKGLWKTTFWS*NCSSLLENERHCDL SCFILNLIKRI*LRAHCS*S*ALLKSLSITLVG RGSGQMNTFSSACEASHYLLLNYYVNEDGHFRN HQWLLALKQYRPDLVLSTCLSNCLHSVVLHNVCL LSFTVHAFNVAHIKHCGSRQEKGWSSAFHYFVS IQATIFI*YK*FMDL*HYNPALFQD*HFFLRKE III*DHEKRLFFVFFFFFFFETGSGCVALTGVQW CKHSSLHNLLGPSDTPTSALQNTGITGVSHCVW PEKAFLRKQIVYLINKIEYIYIAYLKCLKPELF CIF*IFVYT**DLGDGTQV*TWNSPVFRVYASYT *FCA*NRVFV*EDTLQLKRAGFFFLLGSLHKLL YAWCFATCHTRSRVEFSTSGITSVLRNFLISEH FN*GCSNATVSTSPFQV*DVTHLDHKLAFHSAQM FHNSSPKIMKFAV*QLFF*EGGSGSVAQAGVQR CHLDSLQPPPARIQAILLPQAPE*LGLQTPTTT PD*FLYF**RRRGFSMLARLVSN*PQVICPPRPP KVKSTRIIGMSHCAQPI**FF*TYYFGLGCG*S *SSDFLFFGSDPFGKL*T*DKEV*KY*PAADRF YSFRS*GDSKGAPRN*GSSYKLHLTQNSASENVK ESSGNETPQLNKSKFGLFEWFH*ISLQR*QTEA QSAILQEAQHPELVQNVQLTAAEKPPKFEFLFL FLTVYIVK*CKENIYNSESF*CGKRYSKCTINTK II*TVQ*H*FIPLGLLFHTQEDEINSK*HQF*L FLCMSEKMSKLLLNPNNK**CAA*GSTAPLV*S MFHSWVAPFSATQPLVGSILTLSTRNTSRE*INH FKAGVEALALFLSTF*KPSALICIFRAVDQLTY LLREAGPLRNEQGTGASSLFLW*WWWWW*FPEY AIDVGKRLEIFSVSQLRVKKHPKIKSPSFFLCLQ R*QRS*T*PVRTVFPESSENN**LPITRRHSTE *H*LVAVITYGEYYLFENVNILVVLLEIKVAQ* IMILQPFGHHKGNE*E*VFFPRMINSDVER*ID* LSTKCSTPS*EMHGTCATRGKQSKQFF*EQFSP SVTFHYQVPQLEN*PPTPFSLCSVSSRSRLFKP SSNKKGLYDSHWPGITFVSSFFSLCLSPSTHHSF KLSNFVTTPPPHYSRNRTFCF*PLMPT*LPTLL ILHLL*TKLLNDLKKYFFNFPLA*PLLDCWGHM DLRFPSTCPLLIWGLVS*C*WQKQ*KSKALKCPA SNTGKQKYSSTVIYTILHSHLFI*YNGNTKEKV KVSQ*VKDRASFFWNFLNVSYFTLKTTVVQQHF IYHSVSTSKLMAKLHVRRELNFFFFLIANAAVKA ANIFCLKH**PIIRKSCTPVPWYFSLGSKEQRK NKKERGKKKGGGQGRREPSRWFLSCGSDS*GSE GFRACTKSKSSTHSKLQMGLILH*VLQLPYEQDF PSPHPHY*V*INRKAWSEAAP*QKSCSFLPLPF Q*LERGRLSILRAQCSIKNIPLWQEITS*MQH* *LVYRLLPTPTHPLSIPSPAPRSIQLMSLGLSGP QVGAWGVADGFLQIADVEILQGGSSHRLELAKW LWCWATRPTPLSAKWGSWKAILLWGAHRLGEVT *RGS*CCRDGGRATQVRGHGDAQLPRCRELPCHS RALKTKPPRVKSGVGHGIKCVALTYTSSIIL*D TAEQSSVVFIGSLYLPSKGENNPHEREGCHVRV CLCVCS*G*RR

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHE DVGYGVSEGLKSKALSLEKARKEAVTDGLKRAL RSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLT KAKRQDLEPSVEEARYNSCRPNMALGHPQLQQV TSPSRPSHAVIPADQDCSSRSLSSSAVESEATH QRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEA APPAPPVTHSTPVTVSEPLLEKDFLAGVTQELI KTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTS DTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQ TYSADQRTTGNWESHRKSQDMKKRKYDPS*LRL RPHNWTLSQRDFGKLLFGHEIVHRCWRMNVIAI YLASF*TLSRGSD*EPTAVRAEHF*KACPSL**G EALDR*IPFLRLVRLPTIYY*TIMLMKMDILGI TNGSLPSSNIGQTWS*APASAIVYIQLFCITSA FFPLRSMPLMLPTLSTVDHDRKKVGAVLFTTLYQ SRLQSSFNINNLWIYDITILHCFKTDIFS*GRK *SSKTTKKGCFLFFFFFFFLRRGLAVLP*LEFS GANTALSTTSWAQVILPPLPYKIQGLLV*ATVSG QKRHF*ESKSYTLLTK*NIYILLI*NA*NQNCF AFFEYLYTHNETLGMGPKSERGIHLCFVYMPHT HNFVHETEFLYKKIHCS*RGLGFFFS*GRCINCC MPGALRLVTRGHVWNFPLLASRQCSEIF*SQSI SIRDAQTQLFLLPHFRCEM*PTLTINWLFIVLR CFIILPPK**SLQFNNFFFERGGLAL*RRLECSG AILTHCSLRLPGFKQFSCLRLPSSWDYRHPPRR PTNFCIFSRGDGVSPCWPGWSQTDLK*SAHLGL PK*KVLEL*A*AIAPSQFNNFFRRIILGWAVANL EAVTSYFLAQTHLGSCEPEIRKSESTSRQPTVS IPFEVKETAKELPGIRDPVINCT*LKIVPVRM* RNLLEMKPHSSTKASLVYLNGFIK*VYKGNKLKH KAQSYKKRNTQS*CKMYN*RLLRNPQSLNFYFY F*QCTLLNNVRKTFIIQKVFNVEKDTQSVLLTQ K*FKQFSSTSSFL*ACYFIHRRMKSTVNNTSSDF FCV*VKRCQNYY*TQITNDNVLHEAPLLLWYKV CFTLGWPLSQLLSPLLVLSLLSPQEIQAENK*I ILKQGWKPLPFSFLPFKNLQLSFAYLGP*TNLLT Y*GKQGP*GMNRVQEPPASFCGSGGGGGDSQNM PLTWVKGWKYFQFPN*ESRSTPKLRVLASFFVF RDNKDPKHSQLGQYFRKALKIINNYPLQGGIALN NTDWLQLLLTGSIIYLKM*TY*LYF*K*RWHNR L*SCNHLDIIKEMSRNKYSFLE*LIQMWKDR*I NFPQNVVPQAEKCMVHVQQEESNPNSFSESNFLH L*LFTIRFPNLKTSPPPPSLYVQFLLGHVCSSL AQIRKVYMTVTGQASRLFPHSLVFVFHPAHTIL LSCPILLQHPLLTIPETELSVFNH*CQPNFPPYS SYTCSRQSC*MT*KNTFSISLWHNHCWTAGVTW TYDFLPHALY*SGVW*VDANGRSNRSPRH*NVQ QATLGNRSIQVQ*FIQFYIHIYLSNTMEIQRKKL KCLNK*RTELAFFGTF*TFHILLLKLLLSNNIL YITVFPHQN*WQSYMSAGNLIFFFS*LLMLQSK LLISFA*STNDLS*ERVAPQYPGIFHWVLKSKEK IKKRGVKKREEGRVGESPVGGFCPVVRILEGVR DSGHVPKARAPPTRNYRWV*FYIEFSSFLMNKT FLALILITEYR*TGKHGLKLPLDKKAVLSFLSHS NNWSGGDSPF*GLNAVLKTFPSGKK*PVKCSTS S*YIGYFPPPPTPSAFHLLPPDLFS*CL*V*VV RRLEPGGLLMDFCKLLMLKFCKEAVPTGWNWPSG CGAGPPVPLH*APNGVAGKPYCCGGHIDLVK*P REVADAAEMGAEPPRFEVMEMHSCPGAENFLAI PGP*KQNHHVSSLVWAMV*NVWR*HILLV*SCKI QQNSLL*YLLALYIYQVRVKIILMRGRDVMCVC VCVCVVEDDE

Sequence RAD52.bAug05

GGAAGCCCGAGGAGCCGCTTCGTGTCTTCCCAAACCTAAGCGGCGCCCCACCCCCTACTTCTCGTGGCCCGTTACGCCCGGGGCAGCCAAGCGCCGCGGG AGCAGCGCCGGGATCGACCCTGGCTGCTGGGGGGACGCTTTGTCCCAAGAAAGCTGGTTTTTTGCCCCTTAGCTTCGTGGAACGCGGCCGCGAAATTAGT GGTCTTCTGACCTGAGACTCGCGGGGCTGCGCACACGGGCATCGCCGAGGACCCCAGCAATGGACGTCGTGTCTGTGGTGATCCCGCCAGGACGCGGGGA GCGTGGGCGGAAATTAGTGTCCACGTTCCGACCCTGCGCTGTGCCAAACGCTGCTTCGGGAGCTGTTTATTTAGCCTCCGACAGCTAAGCGAATCGGCGG GCGGCTGGGGCCTGGTGCAACCCGATTTTCTCGTGCAGACTCGCAGCCGGCTTTGGTTTTGTTTTTTGTTTTTTGTTTTTTTTTTGGAGCCATGCAGAGG GTAGCTACAGCCATTAATTTAAGGATGTAGATAAGCTATCTCTAAACCAAGAAAGTAAGAATGGTGTGAATTATATTTATTCCCAGCCTTCCAGTGTTTA TTAAAATAATTTTCGGTGTCCTTGAGAGGAGTTAAAAGCCATCATTTTACTCCTGCTTACCTCCGCCACCGTAATTAACCACTTTATACTGCCTCATAAT CCCATTAACAAATTGCAGTCGCTCCTCAGCCTTCACATTCATTCACTCACCAGTTTCTACTGTTTCATCCTGCCCGGACTCTTAACTGTTCCCTCCCAGG ATCGACATGCACTTGGATCATTGCAGTTTCTGGGCCTGCTGTCATCTCCCCCTTCAGTACTTCCCACATATGACTACCAACTGTTAGACGATGTGCTGCT GCCAGACCAGCCTTTCATTTCTTGGACATCCGGCTATTTACCCAGGAAAAGTCATCCTTTGACTATTTTTCATGACCTTTCTCATCTGACTCGTGATCTG CCCATCTTGTTACTCCCTAGCAGTGTGGTAAGAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGC TGCTGGCGGCGGCTCAGTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAA TACATAAGTAGCCGCATGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCT GGGCACACTCCATCACGCAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGA TGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGG CTGAAGCGAGCCCTCAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTC TTGAAGTGGATTTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCC ACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAG AGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAGCAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAG CTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCCTGTGACGCACAGCACTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGT GACTCAAGAATTAATCAAGACTCTTGAAGACAACTCTGAAAAGTGGGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGAC CCAGCCCAGACCTCTGACACATTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTG GATCTTGGGACCTCCAAACTTATAGCGCTGACCAACGCACAACAGGAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCC ATCTTAACTGAGGCTCAGGCCACATAATTGGACTCTGTCACAAAGGGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATG AACGTCATTGCGATTTATCTTGCTTCATTCTGAACCTTATCAAGAGGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTC CATCACTCTAGTAGGGAGAGGCTCTGGACAGATGAATACCTTTTCTTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGAT GGACATTTTAGGAATCACCAATGGCTCCTTGCCCTCAAGCAATATAGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTT TGCATAACGTCTGCCTTCTTTCCTTTACGGTCCATGCCTTTAATGTTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTT TCACTACTTTGTATCAATCCAGGCTACAATCTTCATTTAATATAAATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTT TTCCTAAGGAAGGAAATAATCATCTAAGACCACGAAAAAAGGCTGTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCC TGACTGGAGTTCAGTGGTGCAAACACAGCTCTCTCCACAACCTCTTGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGT GAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTGAGAAAGCAAATCGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTG AAACCAGAATTGTTTTGCATTTTTTGAATATTTGTATACACATAATGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTG TATATGCCTCATACACATAATTTTGTGCATGAAACAGAGTTTTTGTATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTC GCTGCATAAACTGTTGTATGCCTGGTGCTTTGCGACTTGTCACACGAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTT CTGATCTCAGAGCATTTCAATTAGGGATGCTCAAACGCAACTGTTTCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTT TTCATAGTGCTCAGATGTTTCATAATTCTTCCCCCAAAATAATGAAGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCA GGCTGGAGTGCAGCGGTGCCATCTTGACTCATTGCAGCCTCCGCCTGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAG ACACCCACCACGACGCCCGACTAATTTTTGTATTTTTAGTAGAGGAGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCT GCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAGAATTATAGGCATGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCT GGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTATTTTTTGGCTCAGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCG GCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAGGAGACAGCAAAGGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTG CCAGTGAGAATGTGAAGGAATCTTCTGGAAATGAAACCCCACAGCTCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAG GTAACAAACTGAAGCACAAAGCGCAATCTTACAAGAAGCGCAACACCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTT GAATTTTTATTTTTATTTTTAACAGTGTACATTGTTAAATAATGTAAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGT GTACTATTAACACAAAAATAATTTAAACAGTTCAGTAGCACTAGTTCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATA ACACCAGTTCTGACTTTTTTTGTGTATGAGTGAAAAGATGTCAAAATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCT CCTCTGGTATAAAGTATGTTTCACTCTTGGGTGGCCCCTTTCTCAGCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCA GAGAATAAATAAATCATTTTAAAGCAGGGGTGGAAGCCCTTGCCCTTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGT AGACCAACTTACTTACTTACTAAGGGAAGCAGGGCCCTTAAGGAATGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGG TGATTCCCAGAATATGCCATTGACGTGGGTAAAAGGTTGGAAATATTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCT TCTTTCTTTGTCTTCAGAGATAACAAAGATCCTAAACATAGCCAGTTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAG GAGGCATAGCACTGAATAACACTGATTGGTTGCAGTTATTACTTACGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATA AAGGTGGCACAATAGATTATGATCCTGCAACCATTTGGACATCATAAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGG AAAGATAGATAGATTAACTTTCCACAAAATGTAGTACCCCAAGCTGAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTG AGAGCAATTTTCTCCATCTGTGACTTTTCACTATCAGGTTCCCCAACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCA CGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTTTATATGACAGTCACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCA GCACACACCATTCTTTTAAGTTGTCCAATTTTGTTACAACACCCCCTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTA ACTTCCCACCCTACTCATCTTACACCTGCTCTAGACAAAGTTGTTGAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGAC TGCTGGGGTCACATGGACCTACGATTTCCTTCCACATGCCCTTTATTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGG CACTAAAATGTCCAGCAAGCAACACTGGGAAACAGAAGTATTCAAGTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAA TACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTAAAGGACAGAGCTAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTT GTCCAACAACATTTTATATATCACAGTGTTTCCACATCAAAACTGATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTA ATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTTAAAGCACTAATGACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTC TAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGTAAAAAAAAGGGAGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGAT TCTTGAGGGAGTGAGGGATTCAGGGCATGTACCAAAAGCAAGAGCTCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTC CTTATGAACAAGACTTTCCTAGCCCTCATCCTCATTACTGAGTATAGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTT CCTTCCTCTCCCATTCCAATAACTGGAGCGGGGGAGACTCTCCATTCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGT TAAATGCAGCACTAGTAGTTAGTATATAGGCTACTTCCCACCCCCACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTC TAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGGGGTTGCTGATGGATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTT GGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACCCGTCCCACTCCATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGA CTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCTGCAGAGATGGGGGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAG AACTTCCTTGCCATTCCAGGGCCCTGAAAACAAAACCACCACGTGTCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAG TATAATCCTGTAAGATACAGCAGAACAGTCTTCTGTAGTATTTATTGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGA TGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTTGAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

GSPRSRFVSSQT*AAPHPLLLVARYARGSQAPR EQRRDRPWLLGGRFVPRKLVFCPLASWNAAAKLV VF*PETRGAAHTGIAEDPSNGRRVCGDPARTRG AWAEISVHVPTLRCAKRCFGSCLFSLRQLSESA GGWGLVQPDFLVQTRSRLWFCFLFFVFFLEPCRG *LQPLI*GCR*AISKPRK*EWCELYLFPAFQCL LK*FSVSLRGVKSHHFTPAYLRHRN*PLYTAS* SH*QIAVAPQPSHSFTHQFLLFHPARTLNCSLPG STCTWIIAVSGPAVISPFSTSHI*LPTVRRCAA ARPAFHFLDIRLFTQEKSSFDYFS*PFSSDS*S AHLVTP*QCGKRSTRIKMSGTEEAILGGRDSHPA AGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPE YISSRMAGGGQKVCYIEGHRVINLANEMFGYNG WAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKD GSYHEDVGYGVSEGLKSKALSLEKARKEAVTDG LKRALRSFGNALGNCILDKDYLRSLNKLPRQLP LEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHP QLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVE SEATHQRKLRQKQLQQQFRERMEKQQVRVSTPS AEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGV TQELIKTLEDNSEKWAVTPDAGDGVVKPSSRAD PAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKS GSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDP S*LRLRPHNWTLSQRDFGKLLFGHEIVHRCWRM NVIAIYLASF*TLSRGSD*EPTAVRAEHF*KAC PSL**GEALDR*IPFLRLVRLPTIYY*TIMLMKM DILGITNGSLPSSNIGQTWS*APASAIVYIQLF CITSAFFPLRSMPLMLPTLSTVDHDRKKVGAVL FTTLYQSRLQSSFNINNLWIYDITILHCFKTDIF S*GRK*SSKTTKKGCFLFFFFFFFLRRGLAVLP *LEFSGANTALSTTSWAQVILPPLPYKIQGLLV *ATVSGQKRHF*ESKSYTLLTK*NIYILLI*NA* NQNCFAFFEYLYTHNETLGMGPKSERGIHLCFV YMPHTHNFVHETEFLYKKIHCS*RGLGFFFS*G RCINCCMPGALRLVTRGHVWNFPLLASRQCSEIF *SQSISIRDAQTQLFLLPHFRCEM*PTLTINWL FIVLRCFIILPPK**SLQFNNFFFERGGLAL*R RLECSGAILTHCSLRLPGFKQFSCLRLPSSWDYR HPPRRPTNFCIFSRGDGVSPCWPGWSQTDLK*S AHLGLPK*KVLEL*A*AIAPSQFNNFFRRIILG WAVANLEAVTSYFLAQTHLGSCEPEIRKSESTSR QPTVSIPFEVKETAKELPGIRDPVINCT*LKIV PVRM*RNLLEMKPHSSTKASLVYLNGFIK*VYK GNKLKHKAQSYKKRNTQS*CKMYN*RLLRNPQSL NFYFYF*QCTLLNNVRKTFIIQKVFNVEKDTQS VLLTQK*FKQFSSTSSFL*ACYFIHRRMKSTVN NTSSDFFCV*VKRCQNYY*TQITNDNVLHEAPLL LWYKVCFTLGWPLSQLLSPLLVLSLLSPQEIQA ENK*IILKQGWKPLPFSFLPFKNLQLSFAYLGP *TNLLTY*GKQGP*GMNRVQEPPASFCGSGGGGG DSQNMPLTWVKGWKYFQFPN*ESRSTPKLRVLA SFFVFRDNKDPKHSQLGQYFRKALKIINNYPLQ GGIALNNTDWLQLLLTGSIIYLKM*TY*LYF*K* RWHNRL*SCNHLDIIKEMSRNKYSFLE*LIQMW KDR*INFPQNVVPQAEKCMVHVQQEESNPNSFS ESNFLHL*LFTIRFPNLKTSPPPPSLYVQFLLGH VCSSLAQIRKVYMTVTGQASRLFPHSLVFVFHP AHTILLSCPILLQHPLLTIPETELSVFNH*CQP NFPPYSSYTCSRQSC*MT*KNTFSISLWHNHCWT AGVTWTYDFLPHALY*SGVW*VDANGRSNRSPR H*NVQQATLGNRSIQVQ*FIQFYIHIYLSNTME IQRKKLKCLNK*RTELAFFGTF*TFHILLLKLLL SNNILYITVFPHQN*WQSYMSAGNLIFFFS*LL MLQSKLLISFA*STNDLS*ERVAPQYPGIFHWV LKSKEKIKKRGVKKREEGRVGESPVGGFCPVVRI LEGVRDSGHVPKARAPPTRNYRWV*FYIEFSSF LMNKTFLALILITEYR*TGKHGLKLPLDKKAVL SFLSHSNNWSGGDSPF*GLNAVLKTFPSGKK*PV KCSTSS*YIGYFPPPPTPSAFHLLPPDLFS*CL *V*VVRRLEPGGLLMDFCKLLMLKFCKEAVPTG WNWPSGCGAGPPVPLH*APNGVAGKPYCCGGHID LVK*PREVADAAEMGAEPPRFEVMEMHSCPGAE NFLAIPGP*KQNHHVSSLVWAMV*NVWR*HILL V*SCKIQQNSLL*YLLALYIYQVRVKIILMRGRD VMCVCVCVCVVEDDE

Frame 1

EARGAASCLPKPKRRPTPYFSWPVTPGAAKRRG SSAGIDPGCWGDALSQESWFFAP*LRGTRPRN* WSSDLRLAGLRTRASPRTPAMDVVSVVIPPGRGE RGRKLVSTFRPCAVPNAASGAVYLASDS*ANRR AAGAWCNPIFSCRLAAGFGFVFCFLFFFWSHAE GSYSH*FKDVDKLSLNQESKNGVNYIYSQPSSVY *NNFRCP*EELKAIILLLLTSATVINHFILPHN PINKLQSLLSLHIHSLTSFYCFILPGLLTVPSQ DRHALGSLQFLGLLSSPPSVLPTYDYQLLDDVLL PDQPFISWTSGYLPRKSHPLTIFHDLSHLTRDL PILLLPSSVVRGQPESRCLGLRKQFLEDVTAIL LLAAAQCYALDSASTQQKSTRPSRRP*GRGWAQN T*VAAWLAEARRCATLRVIG*LIWPMRCLVTMA GHTPSRSRMWILLTSTMASSTWESVHL*GSS*R MVHIMKMLVMVLVRASSPRLYLWRRQGRRR*QTG *SEPSGVLGMHLETVFWTKTT*DH*ISFHASCL LKWI*LKRRDKILNRLWRRQDTTAADRTWPWDT HSCSR*PPLPDPAML*YRRTRTAAPEA*AHPPWR ARPRTSGSSGRSSCSSSSGSGWRSSRFESPRRQ LRRVRQRLRPLL*RTALL*LSQNHSWRKTSLQE *LKN*SRLLKTTLKSGL*LPMQGMVWSSPRLEQT QPRPLTH*P*TTRW*PRTGLHTAFATRNHKQNL DLGTSKLIALTNAQQETGNLIGRART*RKGNMI HLN*GSGHIIGLCHKGTLENYFLVMKLFIAAGE* TSLRFILLHSEPYQEDLTESPLQLELSTFEKLV HHSSRERLWTDEYLFFGL*GFPLFITELLC**R WTF*ESPMAPCPQAI*ARLGPKHLPQQLSTFSCF A*RLPSFLYGPCL*CCPH*ALWITTGKRLEQCF SLLCINPGYNLHLI*IIYGFMTLQSCIVSRLTF FPKEGNNHLRPRKKAVFCFFFFFFF*DGVWLCCP DWSSVVQTQLSPQPLGPK*YSHLCLTKYRDYWC EPLCLARKGIFEKANRIPY*QNRIYIYCLSEML ETRIVLHFLNICIHIMRPWGWDPSLNVEFTCVSC ICLIHIILCMKQSFCIRRYTAAEEGWVFFSLRV AA*TVVCLVLCDLSHEVTCGIFHFWHHVSAQKF SDLRAFQLGMLKRNCFYFPISGVRCNPP*P*IGF S*CSDVS*FFPQNNEVCSLTTFFLRGGVWLCSA GWSAAVPS*LIAASACQDSSNSPASGSRVAGIT DTHHDARLIFVFLVEETGFLHVGQAGLKLTSSDL PTSASQSKKY*NYRHEPLRPANLIIFLDVLFWA GLWLILKQ*LPIFWLRPIWEAVNLR*GSLKVLA GSRPFLFLSKLRRQQRSSQELGIQL*IALNSK*C Q*ECEGIFWK*NPTAQQKQVWFI*MVSLNKSTK VTN*STKRNLTRSATPRVSAKCTINGC*ETPKV *IFIFIFNSVHC*IM*GKHL*FRKFLMWKKILKV YY*HKNNLNSSVALVHSSRLVISYTGG*NQQ*I TPVLTFFVYE*KDVKIIIKPK*QMIMCCMRLHC SSGIKYVSLLGGPFLSYSAPCWFYPYSLHKKYKQ RINKSF*SRGGSPCPFPFYLLKTFSSHLHI*GR RPTYLLTKGSRALKE*TGYRSLQPLSVVVVVVV VIPRICH*RG*KVGNIFSFPTKSQEAPQN*ES*L LSLSSEITKILNIAS*DSISGKL*K*LITTHYK EA*H*ITLIGCSYYLRGVLFI*KCEHISCTSRN KGGTIDYDPATIWTS*RK*VGISILS*ND*FRCG KIDRLTFHKM*YPKLRNAWYMCNKRKAIQTVFL RAIFSICDFSLSGSPT*KLAPHPLLFMFSFF*V TSVQA*LK*ERFI*QSLARHHVCFLIL*SLSFTQ HTPFF*VVQFCYNTPSSLFPKQNFLFLTTDANL TSHPTHLTPALDKVVE*LKKILFQFPFGITTAG LLGSHGPTISFHMPFIDLGSGKLMLMAEAIEVQG TKMSSKQHWETEVFKYSNLYNFTFTFIYLIQWK YKGKS*SVSISKGQS*LFLELFKRFIFYS*NYC CPTTFYISQCFHIKTDGKVTCPQGT*FFFFPNC* CCSQSC*YLLLKALMTYHKKELHPSTLVFFTGF *RAKKK*KREG*KKGRRAG*ERAQ*VVFVLWFG FLRE*GIQGMYQKQELHPLEITDGFDFTLSSPAS L*TRLS*PSSSLLSIDKQESMV*SCPLTKKLFF PSSPIPITGAGETLHSEGSMQY*KHSPLARNNQ LNAALVVSI*ATSHPHPPPQHSISCPQIYSVNVS RSKWSAGWSLGGC*WISANC*C*NSARRQFPQV GTGQVAVVLGHPSHSIERQMG*LESHTAVGGT* TW*SDLER*LMLQRWGQSHPGSRSWRCTVAQVQR TSLPFQGPENKTTTCQVWCGPWYKMCGANIYF* YNPVRYSRTVFCSIYWLFIFTK*G*K*SS*EGG MSCACVSVCV*LRMTK

Frame 2

KPEEPLRVFPNLSGAPPPTSRGPLRPGQPSAAG AAPGSTLAAGGTLCPKKAGFLPLSFVERGREIS GLLT*DSRGCAHGHRRGPQQWTSCLW*SRQDAG SVGGN*CPRSDPALCQTLLRELFI*PPTAKRIGG RLGPGATRFSRADSQPALVLFFVFCFFFGAMQR VATAINLRM*ISYL*TKKVRMV*IIFIPSLPVF IKIIFGVLERS*KPSFYSCLPPPP*LTTLYCLII PLTNCSRSSAFTFIHSPVSTVSSCPDS*LFPPR IDMHLDHCSFWACCHLPLQYFPHMTTNC*TMCC CQTSLSFLGHPAIYPGKVIL*LFFMTFLI*LVIC PSCYSLAVW*EVNQNQDVWD*GSNSWRT*QPSC CWRRLSVMLWTVPVHSRRVPGHPEGPEAEAGPR IHK*PHGWRRPEGVLH*GSSGN*SGQ*DVWLQWL GTLHHAAECGFC*PQQWQVLRGSLCICEGPAEG WFIS*RCWLWC**GPQVQGFIFGEGKEGGGDRR AEASPQEFWECTWKLYSGQRLPEITK*ASTPVAS *SGFN*SEETRS*TVCGGGKIQQLPTEHGPGTP TAAAGDLPFQTQPCCDTGGPGLQLPKPELIRRG ERGHAPAEAPAEAAAAAVPGADGEAAGSSLHAVS *EE*GSASGPSCDAQHSCNCLRTTPGERLPCRS DSRINQDS*RQL*KVGCDSRCRGWCGQALV*SR PSPDL*HISLEQPDGDPEQDSTQRLPPETTSKIW ILGPPNL*R*PTHNRKLGIS*EEPGHEEKEI*S ILTEAQAT*LDSVTKGLWKTTFWS*NCSSLLEN ERHCDLSCFILNLIKRI*LRAHCS*S*ALLKSLS ITLVGRGSGQMNTFSSACEASHYLLLNYYVNED GHFRNHQWLLALKQYRPDLVLSTCLSNCLHSVV LHNVCLLSFTVHAFNVAHIKHCGSRQEKGWSSAF HYFVSIQATIFI*YK*FMDL*HYNPALFQD*HF FLRKEIII*DHEKRLFFVFFFFFFFETGSGCVA LTGVQWCKHSSLHNLLGPSDTPTSALQNTGITGV SHCVWPEKAFLRKQIVYLINKIEYIYIAYLKCL KPELFCIF*IFVYT**DLGDGTQV*TWNSPVFR VYASYT*FCA*NRVFV*EDTLQLKRAGFFFLLGS LHKLLYAWCFATCHTRSRVEFSTSGITSVLRNF LISEHFN*GCSNATVSTSPFQV*DVTHLDHKLA FHSAQMFHNSSPKIMKFAV*QLFF*EGGSGSVAQ AGVQRCHLDSLQPPPARIQAILLPQAPE*LGLQ TPTTTPD*FLYF**RRRGFSMLARLVSN*PQVI CPPRPPKVKSTRIIGMSHCAQPI**FF*TYYFGL GCG*S*SSDFLFFGSDPFGKL*T*DKEV*KY*P AADRFYSFRS*GDSKGAPRN*GSSYKLHLTQNS ASENVKESSGNETPQLNKSKFGLFEWFH*ISLQR *QTEAQSAILQEAQHPELVQNVQLTAAEKPPKF EFLFLFLTVYIVK*CKENIYNSESF*CGKRYSK CTINTKII*TVQ*H*FIPLGLLFHTQEDEINSK* HQF*LFLCMSEKMSKLLLNPNNK**CAA*GSTA PLV*SMFHSWVAPFSATQPLVGSILTLSTRNTS RE*INHFKAGVEALALFLSTF*KPSALICIFRAV DQLTYLLREAGPLRNEQGTGASSLFLW*WWWWW *FPEYAIDVGKRLEIFSVSQLRVKKHPKIKSPS FFLCLQR*QRS*T*PVRTVFPESSENN**LPITR RHSTE*H*LVAVITYGEYYLFENVNILVVLLEI KVAQ*IMILQPFGHHKGNE*E*VFFPRMINSDV ER*ID*LSTKCSTPS*EMHGTCATRGKQSKQFF* EQFSPSVTFHYQVPQLEN*PPTPFSLCSVSSRS RLFKPSSNKKGLYDSHWPGITFVSSFFSLCLSP STHHSFKLSNFVTTPPPHYSRNRTFCF*PLMPT* LPTLLILHLL*TKLLNDLKKYFFNFPLA*PLLD CWGHMDLRFPSTCPLLIWGLVS*C*WQKQ*KSK ALKCPASNTGKQKYSSTVIYTILHSHLFI*YNGN TKEKVKVSQ*VKDRASFFWNFLNVSYFTLKTTV VQQHFIYHSVSTSKLMAKLHVRRELNFFFFLIA NAAVKAANIFCLKH**PIIRKSCTPVPWYFSLGS KEQRKNKKERGKKKGGGQGRREPSRWFLSCGSD S*GSEGFRACTKSKSSTHSKLQMGLILH*VLQL PYEQDFPSPHPHY*V*INRKAWSEAAP*QKSCSF LPLPFQ*LERGRLSILRAQCSIKNIPLWQEITS *MQH**LVYRLLPTPTHPLSIPSPAPRSIQLMS LGLSGPQVGAWGVADGFLQIADVEILQGGSSHRL ELAKWLWCWATRPTPLSAKWGSWKAILLWGAHR LGEVT*RGS*CCRDGGRATQVRGHGDAQLPRCR ELPCHSRALKTKPPRVKSGVGHGIKCVALTYTSS IIL*DTAEQSSVVFIGSLYLPSKGENNPHEREG CHVRVCLCVCS*G*RR

Sequence RAD52.dAug05

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWILLTSTMASSTWESVHL*GSS*RMVHIMK MLVMVLVRASSPRLYLWRRQGRRR*QTG*SEPS GVLGMHLETVFWTKTT*DH*ISFHASCLLKWI* LKRRDKILNRLWRRQDTTAADRTWPWDTHSCSR* PPLPDPAML*YRRTRTAAPGM

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGWFIS* RCWLWC**GPQVQGFIFGEGKEGGGDRRAEASPQ EFWECTWKLYSGQRLPEITK*ASTPVAS*SGFN *SEETRS*TVCGGGKIQQLPTEHGPGTPTAAAG DLPFQTQPCCDTGGPGLQLPVC

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHE DVGYGVSEGLKSKALSLEKARKEAVTDGLKRAL RSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLT KAKRQDLEPSVEEARYNSCRPNMALGHPQLQQV TSPSRPSHAVIPADQDCSSRY

Sequence RAD52.iAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCA GTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCA TGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCAC GCAGCAGAATGTGGGTGAGTATGCTCTCCAGCAGTGGGGACTTCTGCACTGTCCAGCTCCTGCTGAAAGTCTGCTGTGGGTGAGGAGGTAAGGTAGGTGG ACTCAGCTGCCAGCAGGAGGAATGGTTGTGGCCCAGCGGTCAGTGTCCTCTTCAACTTGCTCTCTTTCCCACTCCTAGATTTTGTTGACCTCAACAATGG CAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGACCTGCTGCCCGGAAGGATCAGTGGGTGGTAGGAGAA GGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGAC GGGCTGAAGCGAGCCCTCAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGC CTCTTGAAGTGGATTTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACA CCCACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGCCGCGGCTGTTGCTCAGGAGCC CGTCCTCGCCGCCGCCCTCTCATGCAGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAG CAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCCTGTGACGCACAGCA CTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATCAAGACTCTTGAAGACAACTCTGAAAAGTGGGC TGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACAACCAGATGGTGACC CAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCAACGCACAACAGGAA ACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACTCTGTCACAAAGGGA CTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAACCTTATCAAGAGGA TCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATGAATACCTTTTCTTC GGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCCTCAAGCAATATAGG CCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCATGCCTTTAATGTTG CCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTCATTTAATATAAATA ATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACGAAAAAAGGCTGTTT TTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCTCCACAACCTCTTGG GCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTGAGAAAGCAAATCGT ATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTGTATACACATAATGA GACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAACAGAGTTTTTGTAT AAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCGACTTGTCACACGAG GTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAAACGCAACTGTTTCT ACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCCCAAAATAATGAAGT TTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTGCAGCCTCCGCCTGC CAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATTTTTAGTAGAGGAGA CGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAGAATTATAGGCATGA GCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTATTTTTTGGCTCAGA CCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAGGAGACAGCAAAGGA GCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGAAACCCCACAGCTCA ACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAAGAAGCGCAACACCC AGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTGTTAAATAATGTAAG GAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCAGTAGCACTAGTTCA TTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAAAAGATGTCAAAATT ATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGGCCCCTTTCTCAGCT ACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGAAGCCCTTGCCCTTT TCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGGCCCTTAAGGAATGA ACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAAGGTTGGAAATATTT TCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTAAACATAGCCAGTTA GGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCAGTTATTACTTACGG GGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCATTTGGACATCATAAA GGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAGTACCCCAAGCTGAG AAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTATCAGGTTCCCCAACT TGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTTTATATGACAGTCAC TGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGTTACAACACCCCCTC CTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAGACAAAGTTGTTGAA TGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCACATGCCCTTTATTG ATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACAGAAGTATTCAAGTA CAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTAAAGGACAGAGCTAG CTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCACATCAAAACTGATG GCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTTAAAGCACTAATGAC CTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGTAAAAAAAAGGGAGG AGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCAAAAGCAAGAGCTCC ACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCATTACTGAGTATAGA TAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGGAGACTCTCCATTCT GAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTACTTCCCACCCCCACC CACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGGGGTTGCTGATGGAT TTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACCCGTCCCACTCCATT GAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCTGCAGAGATGGGGGC AGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAAACCACCACGTGTCA AGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCTGTAGTATTTATTGG CTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTTGAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWVSMLSSSGDFCTVQLLLKVCCG*GGKVGG LSCQQEEWLWPSGQCPLQLALFPTPRFC*PQQW QVLRGSLCICEGPAEGEGME*TCCPEGSVGGRR RMVHIMKMLVMVLVRASSPRLYLWRRQGRRR*QT G*SEPSGVLGMHLETVFWTKTT*DH*ISFHASC LLKWI*LKRRDKILNRLWRRQDTTAADRTWPWD THSCSR*PPLPDPAML*YRRTRTAAPAAAVAQEP VLAAALSCRSLSSSAVESEATHQRKLRQKQLQQ QFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHS TPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWA VTPDAGDGVVKPSSRADPAQTSDTLALNNQMVT QNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTG NWESHRKSQDMKKRKYDPS*LRLRPHNWTLSQRD FGKLLFGHEIVHRCWRMNVIAIYLASF*TLSRG SD*EPTAVRAEHF*KACPSL**GEALDR*IPFL RLVRLPTIYY*TIMLMKMDILGITNGSLPSSNIG QTWS*APASAIVYIQLFCITSAFFPLRSMPLML PTLSTVDHDRKKVGAVLFTTLYQSRLQSSFNIN NLWIYDITILHCFKTDIFS*GRK*SSKTTKKGCF LFFFFFFFLRRGLAVLP*LEFSGANTALSTTSW AQVILPPLPYKIQGLLV*ATVSGQKRHF*ESKS YTLLTK*NIYILLI*NA*NQNCFAFFEYLYTHNE TLGMGPKSERGIHLCFVYMPHTHNFVHETEFLY KKIHCS*RGLGFFFS*GRCINCCMPGALRLVTR GHVWNFPLLASRQCSEIF*SQSISIRDAQTQLFL LPHFRCEM*PTLTINWLFIVLRCFIILPPK**S LQFNNFFFERGGLAL*RRLECSGAILTHCSLRL PGFKQFSCLRLPSSWDYRHPPRRPTNFCIFSRGD GVSPCWPGWSQTDLK*SAHLGLPK*KVLEL*A* AIAPSQFNNFFRRIILGWAVANLEAVTSYFLAQ THLGSCEPEIRKSESTSRQPTVSIPFEVKETAKE LPGIRDPVINCT*LKIVPVRM*RNLLEMKPHSS TKASLVYLNGFIK*VYKGNKLKHKAQSYKKRNT QS*CKMYN*RLLRNPQSLNFYFYF*QCTLLNNVR KTFIIQKVFNVEKDTQSVLLTQK*FKQFSSTSS FL*ACYFIHRRMKSTVNNTSSDFFCV*VKRCQN YY*TQITNDNVLHEAPLLLWYKVCFTLGWPLSQL LSPLLVLSLLSPQEIQAENK*IILKQGWKPLPF SFLPFKNLQLSFAYLGP*TNLLTY*GKQGP*GM NRVQEPPASFCGSGGGGGDSQNMPLTWVKGWKYF QFPN*ESRSTPKLRVLASFFVFRDNKDPKHSQL GQYFRKALKIINNYPLQGGIALNNTDWLQLLLT GSIIYLKM*TY*LYF*K*RWHNRL*SCNHLDIIK EMSRNKYSFLE*LIQMWKDR*INFPQNVVPQAE KCMVHVQQEESNPNSFSESNFLHL*LFTIRFPN LKTSPPPPSLYVQFLLGHVCSSLAQIRKVYMTVT GQASRLFPHSLVFVFHPAHTILLSCPILLQHPL LTIPETELSVFNH*CQPNFPPYSSYTCSRQSC* MT*KNTFSISLWHNHCWTAGVTWTYDFLPHALY* SGVW*VDANGRSNRSPRH*NVQQATLGNRSIQV Q*FIQFYIHIYLSNTMEIQRKKLKCLNK*RTEL AFFGTF*TFHILLLKLLLSNNILYITVFPHQN*W QSYMSAGNLIFFFS*LLMLQSKLLISFA*STND LS*ERVAPQYPGIFHWVLKSKEKIKKRGVKKRE EGRVGESPVGGFCPVVRILEGVRDSGHVPKARAP PTRNYRWV*FYIEFSSFLMNKTFLALILITEYR *TGKHGLKLPLDKKAVLSFLSHSNNWSGGDSPF *GLNAVLKTFPSGKK*PVKCSTSS*YIGYFPPPP TPSAFHLLPPDLFS*CL*V*VVRRLEPGGLLMD FCKLLMLKFCKEAVPTGWNWPSGCGAGPPVPLH *APNGVAGKPYCCGGHIDLVK*PREVADAAEMGA EPPRFEVMEMHSCPGAENFLAIPGP*KQNHHVS SLVWAMV*NVWR*HILLV*SCKIQQNSLL*YLL ALYIYQVRVKIILMRGRDVMCVCVCVCVVEDDE

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECG*VCSPAVGTSALSSSC*KSAVGEEVR*V DSAASRRNGCGPAVSVLFNLLSFPLLDFVDLNNG KFYVGVCAFVRVQLKVRGWSRPAARKDQWVVGE GWFIS*RCWLWC**GPQVQGFIFGEGKEGGGDR RAEASPQEFWECTWKLYSGQRLPEITK*ASTPVA S*SGFN*SEETRS*TVCGGGKIQQLPTEHGPGT PTAAAGDLPFQTQPCCDTGGPGLQLPPRLLLRS PSSPPPSHAEA*AHPPWRARPRTSGSSGRSSCSS SSGSGWRSSRFESPRRQLRRVRQRLRPLL*RTA LL*LSQNHSWRKTSLQE*LKN*SRLLKTTLKSG L*LPMQGMVWSSPRLEQTQPRPLTH*P*TTRW*P RTGLHTAFATRNHKQNLDLGTSKLIALTNAQQE TGNLIGRART*RKGNMIHLN*GSGHIIGLCHKG TLENYFLVMKLFIAAGE*TSLRFILLHSEPYQED LTESPLQLELSTFEKLVHHSSRERLWTDEYLFF GL*GFPLFITELLC**RWTF*ESPMAPCPQAI* ARLGPKHLPQQLSTFSCFA*RLPSFLYGPCL*CC PH*ALWITTGKRLEQCFSLLCINPGYNLHLI*I IYGFMTLQSCIVSRLTFFPKEGNNHLRPRKKAV FCFFFFFFF*DGVWLCCPDWSSVVQTQLSPQPLG PK*YSHLCLTKYRDYWCEPLCLARKGIFEKANR IPY*QNRIYIYCLSEMLETRIVLHFLNICIHIM RPWGWDPSLNVEFTCVSCICLIHIILCMKQSFCI RRYTAAEEGWVFFSLRVAA*TVVCLVLCDLSHE VTCGIFHFWHHVSAQKFSDLRAFQLGMLKRNCF YFPISGVRCNPP*P*IGFS*CSDVS*FFPQNNEV CSLTTFFLRGGVWLCSAGWSAAVPS*LIAASAC QDSSNSPASGSRVAGITDTHHDARLIFVFLVEE TGFLHVGQAGLKLTSSDLPTSASQSKKY*NYRHE PLRPANLIIFLDVLFWAGLWLILKQ*LPIFWLR PIWEAVNLR*GSLKVLAGSRPFLFLSKLRRQQR SSQELGIQL*IALNSK*CQ*ECEGIFWK*NPTAQ QKQVWFI*MVSLNKSTKVTN*STKRNLTRSATP RVSAKCTINGC*ETPKV*IFIFIFNSVHC*IM* GKHL*FRKFLMWKKILKVYY*HKNNLNSSVALVH SSRLVISYTGG*NQQ*ITPVLTFFVYE*KDVKI IIKPK*QMIMCCMRLHCSSGIKYVSLLGGPFLS YSAPCWFYPYSLHKKYKQRINKSF*SRGGSPCPF PFYLLKTFSSHLHI*GRRPTYLLTKGSRALKE* TGYRSLQPLSVVVVVVVVIPRICH*RG*KVGNI FSFPTKSQEAPQN*ES*LLSLSSEITKILNIAS* DSISGKL*K*LITTHYKEA*H*ITLIGCSYYLR GVLFI*KCEHISCTSRNKGGTIDYDPATIWTS* RK*VGISILS*ND*FRCGKIDRLTFHKM*YPKLR NAWYMCNKRKAIQTVFLRAIFSICDFSLSGSPT *KLAPHPLLFMFSFF*VTSVQA*LK*ERFI*QS LARHHVCFLIL*SLSFTQHTPFF*VVQFCYNTPS SLFPKQNFLFLTTDANLTSHPTHLTPALDKVVE *LKKILFQFPFGITTAGLLGSHGPTISFHMPFI DLGSGKLMLMAEAIEVQGTKMSSKQHWETEVFKY SNLYNFTFTFIYLIQWKYKGKS*SVSISKGQS* LFLELFKRFIFYS*NYCCPTTFYISQCFHIKTD GKVTCPQGT*FFFFPNC*CCSQSC*YLLLKALMT YHKKELHPSTLVFFTGF*RAKKK*KREG*KKGR RAG*ERAQ*VVFVLWFGFLRE*GIQGMYQKQEL HPLEITDGFDFTLSSPASL*TRLS*PSSSLLSID KQESMV*SCPLTKKLFFPSSPIPITGAGETLHS EGSMQY*KHSPLARNNQLNAALVVSI*ATSHPH PPPQHSISCPQIYSVNVSRSKWSAGWSLGGC*WI SANC*C*NSARRQFPQVGTGQVAVVLGHPSHSI ERQMG*LESHTAVGGT*TW*SDLER*LMLQRWG QSHPGSRSWRCTVAQVQRTSLPFQGPENKTTTCQ VWCGPWYKMCGANIYF*YNPVRYSRTVFCSIYW LFIFTK*G*K*SS*EGGMSCACVSVCV*LRMTK

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVGEYALQQWGLLHCPAPAESLLWVRR*GRW TQLPAGGMVVAQRSVSSSTCSLSHS*ILLTSTM ASSTWESVHL*GSS*R*GDGVDLLPGRISGW*EK DGSYHEDVGYGVSEGLKSKALSLEKARKEAVTD GLKRALRSFGNALGNCILDKDYLRSLNKLPRQL PLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGH PQLQQVTSPSRPSHAVIPADQDCSSRRGCCSGA RPRRRPLMQKPELIRRGERGHAPAEAPAEAAAA AVPGADGEAAGSSLHAVS*EE*GSASGPSCDAQH SCNCLRTTPGERLPCRSDSRINQDS*RQL*KVG CDSRCRGWCGQALV*SRPSPDL*HISLEQPDGD PEQDSTQRLPPETTSKIWILGPPNL*R*PTHNRK LGIS*EEPGHEEKEI*SILTEAQAT*LDSVTKG LWKTTFWS*NCSSLLENERHCDLSCFILNLIKR I*LRAHCS*S*ALLKSLSITLVGRGSGQMNTFSS ACEASHYLLLNYYVNEDGHFRNHQWLLALKQYR PDLVLSTCLSNCLHSVVLHNVCLLSFTVHAFNV AHIKHCGSRQEKGWSSAFHYFVSIQATIFI*YK* FMDL*HYNPALFQD*HFFLRKEIII*DHEKRLF FVFFFFFFFETGSGCVALTGVQWCKHSSLHNLL GPSDTPTSALQNTGITGVSHCVWPEKAFLRKQIV YLINKIEYIYIAYLKCLKPELFCIF*IFVYT** DLGDGTQV*TWNSPVFRVYASYT*FCA*NRVFV *EDTLQLKRAGFFFLLGSLHKLLYAWCFATCHTR SRVEFSTSGITSVLRNFLISEHFN*GCSNATVS TSPFQV*DVTHLDHKLAFHSAQMFHNSSPKIMK FAV*QLFF*EGGSGSVAQAGVQRCHLDSLQPPPA RIQAILLPQAPE*LGLQTPTTTPD*FLYF**RR RGFSMLARLVSN*PQVICPPRPPKVKSTRIIGM SHCAQPI**FF*TYYFGLGCG*S*SSDFLFFGSD PFGKL*T*DKEV*KY*PAADRFYSFRS*GDSKG APRN*GSSYKLHLTQNSASENVKESSGNETPQL NKSKFGLFEWFH*ISLQR*QTEAQSAILQEAQHP ELVQNVQLTAAEKPPKFEFLFLFLTVYIVK*CK ENIYNSESF*CGKRYSKCTINTKII*TVQ*H*F IPLGLLFHTQEDEINSK*HQF*LFLCMSEKMSKL LLNPNNK**CAA*GSTAPLV*SMFHSWVAPFSA TQPLVGSILTLSTRNTSRE*INHFKAGVEALAL FLSTF*KPSALICIFRAVDQLTYLLREAGPLRNE QGTGASSLFLW*WWWWW*FPEYAIDVGKRLEIF SVSQLRVKKHPKIKSPSFFLCLQR*QRS*T*PV RTVFPESSENN**LPITRRHSTE*H*LVAVITYG EYYLFENVNILVVLLEIKVAQ*IMILQPFGHHK GNE*E*VFFPRMINSDVER*ID*LSTKCSTPS* EMHGTCATRGKQSKQFF*EQFSPSVTFHYQVPQL EN*PPTPFSLCSVSSRSRLFKPSSNKKGLYDSH WPGITFVSSFFSLCLSPSTHHSFKLSNFVTTPP PHYSRNRTFCF*PLMPT*LPTLLILHLL*TKLLN DLKKYFFNFPLA*PLLDCWGHMDLRFPSTCPLL IWGLVS*C*WQKQ*KSKALKCPASNTGKQKYSS TVIYTILHSHLFI*YNGNTKEKVKVSQ*VKDRAS FFWNFLNVSYFTLKTTVVQQHFIYHSVSTSKLM AKLHVRRELNFFFFLIANAAVKAANIFCLKH** PIIRKSCTPVPWYFSLGSKEQRKNKKERGKKKGG GQGRREPSRWFLSCGSDS*GSEGFRACTKSKSS THSKLQMGLILH*VLQLPYEQDFPSPHPHY*V* INRKAWSEAAP*QKSCSFLPLPFQ*LERGRLSIL RAQCSIKNIPLWQEITS*MQH**LVYRLLPTPT HPLSIPSPAPRSIQLMSLGLSGPQVGAWGVADG FLQIADVEILQGGSSHRLELAKWLWCWATRPTPL SAKWGSWKAILLWGAHRLGEVT*RGS*CCRDGG RATQVRGHGDAQLPRCRELPCHSRALKTKPPRV KSGVGHGIKCVALTYTSSIIL*DTAEQSSVVFIG SLYLPSKGENNPHEREGCHVRVCLCVCS*G*RR

Sequence RAD52.cAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCCCCAGTGACAGCTGGAGTGGACAAAGTGACCAAGGGCCTCAAGGACACACAAGGCCAAGAAGATTTGAAGTGCTGTAGGTCAAC CAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCAGTGTTATGCTTTGGACAGTGCCAG TACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCATGGCTGGCGGAGGCCAGAAGGTGT GCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCACGCAGCAGAATGTGGATTTTGTTGA CCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGACCTGCTGCCCGGAAGGATCAGTGG GTGGTAGGAGAAGGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAG GCGGTGACAGACGGGCTGAAGCGAGCCCTCAGGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTTCTCCGTGCACTCAGTCATGTGTG CCGGAGGCCTTCCCACTCCTACTGCGAGTGCACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTACGCACAGGAGTTTTGGGAATGCAC TTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATTTAACTAAAGCGAAGAGACAAGA TCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCC AGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGA AGCAGCTGCAGCAGCAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCC TGTGACGCACAGCACTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATCAAGACTCTTGAAGACAAC TCTGAAAAGTGGGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACA ACCAGATGGTGACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCA ACGCACAACAGGAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACT CTGTCACAAAGGGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAA CCTTATCAAGAGGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATG AATACCTTTTCTTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCC TCAAGCAATATAGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCA TGCCTTTAATGTTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTC ATTTAATATAAATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACG AAAAAAGGCTGTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCT CCACAACCTCTTGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTG AGAAAGCAAATCGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTG TATACACATAATGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAA CAGAGTTTTTGTATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCG ACTTGTCACACGAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAA ACGCAACTGTTTCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCC CAAAATAATGAAGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTG CAGCCTCCGCCTGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATT TTTAGTAGAGGAGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAG AATTATAGGCATGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTA TTTTTTGGCTCAGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAG GAGACAGCAAAGGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGA AACCCCACAGCTCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAA GAAGCGCAACACCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTG TTAAATAATGTAAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCA GTAGCACTAGTTCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAA AAGATGTCAAAATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGG CCCCTTTCTCAGCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGA AGCCCTTGCCCTTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGG CCCTTAAGGAATGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAA GGTTGGAAATATTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTA AACATAGCCAGTTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCA GTTATTACTTACGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCAT TTGGACATCATAAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAG TACCCCAAGCTGAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTAT CAGGTTCCCCAACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTT TATATGACAGTCACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGT TACAACACCCCCTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAG ACAAAGTTGTTGAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCA CATGCCCTTTATTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACA GAAGTATTCAAGTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTA AAGGACAGAGCTAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCA CATCAAAACTGATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTT AAAGCACTAATGACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGT AAAAAAAAGGGAGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCA AAAGCAAGAGCTCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCA TTACTGAGTATAGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGG AGACTCTCCATTCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTAC TTCCCACCCCCACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGG GGTTGCTGATGGATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACC CGTCCCACTCCATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCT GCAGAGATGGGGGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAA ACCACCACGTGTCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCT GTAGTATTTATTGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTT GAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAPVTAGVDKVTKGLKDTQGQEDLKCCRST RIKMSGTEEAILGGRDSHPAAGGGSVLCFGQCQ YTAEEYQAIQKALRQRLGPEYISSRMAGGGQKV CYIEGHRVINLANEMFGYNGWAHSITQQNVDFVD LNNGKFYVGVCAFVRVQLKVRGWSRPAARKDQW VVGEGWFIS*RCWLWC**GPQVQGFIFGEGKEG GGDRRAEASPQASLAGSKW*NPVLSLLRALSHVC RRPSHSYCECTDSTLLSVLLSSPPLRTGVLGMH LETVFWTKTT*DH*ISFHASCLLKWI*LKRRDK ILNRLWRRQDTTAADRTWPWDTHSCSR*PPLPDP AML*YRRTRTAAPEA*AHPPWRARPRTSGSSGR SSCSSSSGSGWRSSRFESPRRQLRRVRQRLRPL L*RTALL*LSQNHSWRKTSLQE*LKN*SRLLKTT LKSGL*LPMQGMVWSSPRLEQTQPRPLTH*P*T TRW*PRTGLHTAFATRNHKQNLDLGTSKLIALT NAQQETGNLIGRART*RKGNMIHLN*GSGHIIGL CHKGTLENYFLVMKLFIAAGE*TSLRFILLHSE PYQEDLTESPLQLELSTFEKLVHHSSRERLWTD EYLFFGL*GFPLFITELLC**RWTF*ESPMAPCP QAI*ARLGPKHLPQQLSTFSCFA*RLPSFLYGP CL*CCPH*ALWITTGKRLEQCFSLLCINPGYNL HLI*IIYGFMTLQSCIVSRLTFFPKEGNNHLRPR KKAVFCFFFFFFF*DGVWLCCPDWSSVVQTQLS PQPLGPK*YSHLCLTKYRDYWCEPLCLARKGIF EKANRIPY*QNRIYIYCLSEMLETRIVLHFLNIC IHIMRPWGWDPSLNVEFTCVSCICLIHIILCMK QSFCIRRYTAAEEGWVFFSLRVAA*TVVCLVLC DLSHEVTCGIFHFWHHVSAQKFSDLRAFQLGMLK RNCFYFPISGVRCNPP*P*IGFS*CSDVS*FFP QNNEVCSLTTFFLRGGVWLCSAGWSAAVPS*LI AASACQDSSNSPASGSRVAGITDTHHDARLIFVF LVEETGFLHVGQAGLKLTSSDLPTSASQSKKY* NYRHEPLRPANLIIFLDVLFWAGLWLILKQ*LP IFWLRPIWEAVNLR*GSLKVLAGSRPFLFLSKLR RQQRSSQELGIQL*IALNSK*CQ*ECEGIFWK* NPTAQQKQVWFI*MVSLNKSTKVTN*STKRNLT RSATPRVSAKCTINGC*ETPKV*IFIFIFNSVHC *IM*GKHL*FRKFLMWKKILKVYY*HKNNLNSS VALVHSSRLVISYTGG*NQQ*ITPVLTFFVYE* KDVKIIIKPK*QMIMCCMRLHCSSGIKYVSLLGG PFLSYSAPCWFYPYSLHKKYKQRINKSF*SRGG SPCPFPFYLLKTFSSHLHI*GRRPTYLLTKGSR ALKE*TGYRSLQPLSVVVVVVVVIPRICH*RG*K VGNIFSFPTKSQEAPQN*ES*LLSLSSEITKIL NIAS*DSISGKL*K*LITTHYKEA*H*ITLIGC SYYLRGVLFI*KCEHISCTSRNKGGTIDYDPATI WTS*RK*VGISILS*ND*FRCGKIDRLTFHKM* YPKLRNAWYMCNKRKAIQTVFLRAIFSICDFSL SGSPT*KLAPHPLLFMFSFF*VTSVQA*LK*ERF I*QSLARHHVCFLIL*SLSFTQHTPFF*VVQFC YNTPSSLFPKQNFLFLTTDANLTSHPTHLTPAL DKVVE*LKKILFQFPFGITTAGLLGSHGPTISFH MPFIDLGSGKLMLMAEAIEVQGTKMSSKQHWET EVFKYSNLYNFTFTFIYLIQWKYKGKS*SVSIS KGQS*LFLELFKRFIFYS*NYCCPTTFYISQCFH IKTDGKVTCPQGT*FFFFPNC*CCSQSC*YLLL KALMTYHKKELHPSTLVFFTGF*RAKKK*KREG *KKGRRAG*ERAQ*VVFVLWFGFLRE*GIQGMYQ KQELHPLEITDGFDFTLSSPASL*TRLS*PSSS LLSIDKQESMV*SCPLTKKLFFPSSPIPITGAG ETLHSEGSMQY*KHSPLARNNQLNAALVVSI*AT SHPHPPPQHSISCPQIYSVNVSRSKWSAGWSLG GC*WISANC*C*NSARRQFPQVGTGQVAVVLGH PSHSIERQMG*LESHTAVGGT*TW*SDLER*LML QRWGQSHPGSRSWRCTVAQVQRTSLPFQGPENK TTTCQVWCGPWYKMCGANIYF*YNPVRYSRTVF CSIYWLFIFTK*G*K*SS*EGGMSCACVSVCV*L RMTK

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAPQ*QLEWTK*PRASRTHKAKKI*SAVGQ PESRCLGLRKQFLEDVTAILLLAAAQCYALDSAS TQQKSTRPSRRP*GRGWAQNT*VAAWLAEARRC ATLRVIG*LIWPMRCLVTMAGHTPSRSRMWILL TSTMASSTWESVHL*GSS*R*GDGVDLLPGRISG W*EKDGSYHEDVGYGVSEGLKSKALSLEKARKE AVTDGLKRALRLPLLGVSGRILYSLFSVHSVMC AGGLPTPTASAQTAPSSPCSSAVLRYAQEFWECT WKLYSGQRLPEITK*ASTPVAS*SGFN*SEETR S*TVCGGGKIQQLPTEHGPGTPTAAAGDLPFQT QPCCDTGGPGLQLPKPELIRRGERGHAPAEAPAE AAAAAVPGADGEAAGSSLHAVS*EE*GSASGPS CDAQHSCNCLRTTPGERLPCRSDSRINQDS*RQ L*KVGCDSRCRGWCGQALV*SRPSPDL*HISLEQ PDGDPEQDSTQRLPPETTSKIWILGPPNL*R*P THNRKLGIS*EEPGHEEKEI*SILTEAQAT*LD SVTKGLWKTTFWS*NCSSLLENERHCDLSCFILN LIKRI*LRAHCS*S*ALLKSLSITLVGRGSGQM NTFSSACEASHYLLLNYYVNEDGHFRNHQWLLA LKQYRPDLVLSTCLSNCLHSVVLHNVCLLSFTVH AFNVAHIKHCGSRQEKGWSSAFHYFVSIQATIF I*YK*FMDL*HYNPALFQD*HFFLRKEIII*DH EKRLFFVFFFFFFFETGSGCVALTGVQWCKHSSL HNLLGPSDTPTSALQNTGITGVSHCVWPEKAFL RKQIVYLINKIEYIYIAYLKCLKPELFCIF*IF VYT**DLGDGTQV*TWNSPVFRVYASYT*FCA*N RVFV*EDTLQLKRAGFFFLLGSLHKLLYAWCFA TCHTRSRVEFSTSGITSVLRNFLISEHFN*GCS NATVSTSPFQV*DVTHLDHKLAFHSAQMFHNSSP KIMKFAV*QLFF*EGGSGSVAQAGVQRCHLDSL QPPPARIQAILLPQAPE*LGLQTPTTTPD*FLY F**RRRGFSMLARLVSN*PQVICPPRPPKVKSTR IIGMSHCAQPI**FF*TYYFGLGCG*S*SSDFL FFGSDPFGKL*T*DKEV*KY*PAADRFYSFRS* GDSKGAPRN*GSSYKLHLTQNSASENVKESSGNE TPQLNKSKFGLFEWFH*ISLQR*QTEAQSAILQ EAQHPELVQNVQLTAAEKPPKFEFLFLFLTVYI VK*CKENIYNSESF*CGKRYSKCTINTKII*TVQ *H*FIPLGLLFHTQEDEINSK*HQF*LFLCMSE KMSKLLLNPNNK**CAA*GSTAPLV*SMFHSWV APFSATQPLVGSILTLSTRNTSRE*INHFKAGVE ALALFLSTF*KPSALICIFRAVDQLTYLLREAG PLRNEQGTGASSLFLW*WWWWW*FPEYAIDVGK RLEIFSVSQLRVKKHPKIKSPSFFLCLQR*QRS* T*PVRTVFPESSENN**LPITRRHSTE*H*LVA VITYGEYYLFENVNILVVLLEIKVAQ*IMILQP FGHHKGNE*E*VFFPRMINSDVER*ID*LSTKCS TPS*EMHGTCATRGKQSKQFF*EQFSPSVTFHY QVPQLEN*PPTPFSLCSVSSRSRLFKPSSNKKG LYDSHWPGITFVSSFFSLCLSPSTHHSFKLSNFV TTPPPHYSRNRTFCF*PLMPT*LPTLLILHLL* TKLLNDLKKYFFNFPLA*PLLDCWGHMDLRFPS TCPLLIWGLVS*C*WQKQ*KSKALKCPASNTGKQ KYSSTVIYTILHSHLFI*YNGNTKEKVKVSQ*V KDRASFFWNFLNVSYFTLKTTVVQQHFIYHSVS TSKLMAKLHVRRELNFFFFLIANAAVKAANIFCL KH**PIIRKSCTPVPWYFSLGSKEQRKNKKERG KKKGGGQGRREPSRWFLSCGSDS*GSEGFRACT KSKSSTHSKLQMGLILH*VLQLPYEQDFPSPHPH Y*V*INRKAWSEAAP*QKSCSFLPLPFQ*LERG RLSILRAQCSIKNIPLWQEITS*MQH**LVYRL LPTPTHPLSIPSPAPRSIQLMSLGLSGPQVGAWG VADGFLQIADVEILQGGSSHRLELAKWLWCWAT RPTPLSAKWGSWKAILLWGAHRLGEVT*RGS*C CRDGGRATQVRGHGDAQLPRCRELPCHSRALKTK PPRVKSGVGHGIKCVALTYTSSIIL*DTAEQSS VVFIGSLYLPSKGENNPHEREGCHVRVCLCVCS *G*RR

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRPSDSWSGQSDQGPQGHTRPRRFEVL*VN QNQDVWD*GSNSWRT*QPSCCWRRLSVMLWTVP VHSRRVPGHPEGPEAEAGPRIHK*PHGWRRPEGV LH*GSSGN*SGQ*DVWLQWLGTLHHAAECGFC* PQQWQVLRGSLCICEGPAEGEGME*TCCPEGSV GGRRRMVHIMKMLVMVLVRASSPRLYLWRRQGRR R*QTG*SEPSGFPCWE*VVESCTLSSPCTQSCV PEAFPLLLRVHRQHPPLRAPQQSSATHRSFGNA LGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQD LEPSVEEARYNSCRPNMALGHPQLQQVTSPSRP SHAVIPADQDCSSRSLSSSAVESEATHQRKLRQ KQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPP VTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDN SEKWAVTPDAGDGVVKPSSRADPAQTSDTLALN NQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQ RTTGNWESHRKSQDMKKRKYDPS*LRLRPHNWT LSQRDFGKLLFGHEIVHRCWRMNVIAIYLASF* TLSRGSD*EPTAVRAEHF*KACPSL**GEALDR* IPFLRLVRLPTIYY*TIMLMKMDILGITNGSLP SSNIGQTWS*APASAIVYIQLFCITSAFFPLRS MPLMLPTLSTVDHDRKKVGAVLFTTLYQSRLQSS FNINNLWIYDITILHCFKTDIFS*GRK*SSKTT KKGCFLFFFFFFFLRRGLAVLP*LEFSGANTAL STTSWAQVILPPLPYKIQGLLV*ATVSGQKRHF* ESKSYTLLTK*NIYILLI*NA*NQNCFAFFEYL YTHNETLGMGPKSERGIHLCFVYMPHTHNFVHE TEFLYKKIHCS*RGLGFFFS*GRCINCCMPGALR LVTRGHVWNFPLLASRQCSEIF*SQSISIRDAQ TQLFLLPHFRCEM*PTLTINWLFIVLRCFIILP PK**SLQFNNFFFERGGLAL*RRLECSGAILTHC SLRLPGFKQFSCLRLPSSWDYRHPPRRPTNFCI FSRGDGVSPCWPGWSQTDLK*SAHLGLPK*KVL EL*A*AIAPSQFNNFFRRIILGWAVANLEAVTSY FLAQTHLGSCEPEIRKSESTSRQPTVSIPFEVK ETAKELPGIRDPVINCT*LKIVPVRM*RNLLEM KPHSSTKASLVYLNGFIK*VYKGNKLKHKAQSYK KRNTQS*CKMYN*RLLRNPQSLNFYFYF*QCTL LNNVRKTFIIQKVFNVEKDTQSVLLTQK*FKQF SSTSSFL*ACYFIHRRMKSTVNNTSSDFFCV*VK RCQNYY*TQITNDNVLHEAPLLLWYKVCFTLGW PLSQLLSPLLVLSLLSPQEIQAENK*IILKQGW KPLPFSFLPFKNLQLSFAYLGP*TNLLTY*GKQG P*GMNRVQEPPASFCGSGGGGGDSQNMPLTWVK GWKYFQFPN*ESRSTPKLRVLASFFVFRDNKDP KHSQLGQYFRKALKIINNYPLQGGIALNNTDWLQ LLLTGSIIYLKM*TY*LYF*K*RWHNRL*SCNH LDIIKEMSRNKYSFLE*LIQMWKDR*INFPQNV VPQAEKCMVHVQQEESNPNSFSESNFLHL*LFTI RFPNLKTSPPPPSLYVQFLLGHVCSSLAQIRKV YMTVTGQASRLFPHSLVFVFHPAHTILLSCPIL LQHPLLTIPETELSVFNH*CQPNFPPYSSYTCSR QSC*MT*KNTFSISLWHNHCWTAGVTWTYDFLP HALY*SGVW*VDANGRSNRSPRH*NVQQATLGN RSIQVQ*FIQFYIHIYLSNTMEIQRKKLKCLNK* RTELAFFGTF*TFHILLLKLLLSNNILYITVFP HQN*WQSYMSAGNLIFFFS*LLMLQSKLLISFA *STNDLS*ERVAPQYPGIFHWVLKSKEKIKKRGV KKREEGRVGESPVGGFCPVVRILEGVRDSGHVP KARAPPTRNYRWV*FYIEFSSFLMNKTFLALIL ITEYR*TGKHGLKLPLDKKAVLSFLSHSNNWSGG DSPF*GLNAVLKTFPSGKK*PVKCSTSS*YIGY FPPPPTPSAFHLLPPDLFS*CL*V*VVRRLEPG GLLMDFCKLLMLKFCKEAVPTGWNWPSGCGAGPP VPLH*APNGVAGKPYCCGGHIDLVK*PREVADA AEMGAEPPRFEVMEMHSCPGAENFLAIPGP*KQ NHHVSSLVWAMV*NVWR*HILLV*SCKIQQNSLL *YLLALYIYQVRVKIILMRGRDVMCVCVCVCVV EDDE

Sequence RAD52.fAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCA GTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCA TGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCAC GCAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGATGGTTCATATCATGAA GATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCA GGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTTCTCCGTGCACTCAGTCATGTGTGCCGGAGGCCTTCCCACTCCTACTGCGAGTGC ACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTACGCACAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCT GAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATTTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATAC AACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACT GCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAGCAGTTCCGGGAGCGGAT GGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCCTGTGACGCACAGCACTCCTGTAACTGTCTCA GAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATCAGTAAATACCTTGAAATGCTTAGACTCTTGAAGACAACTCTGAAAAGTG GGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACAACCAGATGGTG ACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCAACGCACAACAG GAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACTCTGTCACAAAG GGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAACCTTATCAAGA GGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATGAATACCTTTTC TTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCCTCAAGCAATAT AGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCATGCCTTTAATG TTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTCATTTAATATAA ATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACGAAAAAAGGCTG TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCTCCACAACCTCT TGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTGAGAAAGCAAAT CGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTGTATACACATAA TGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAACAGAGTTTTTG TATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCGACTTGTCACAC GAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAAACGCAACTGTT TCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCCCAAAATAATGA AGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTGCAGCCTCCGCC TGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATTTTTAGTAGAGG AGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAGAATTATAGGCA TGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTATTTTTTGGCTC AGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAGGAGACAGCAAA GGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGAAACCCCACAGC TCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAAGAAGCGCAACA CCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTGTTAAATAATGT AAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCAGTAGCACTAGT TCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAAAAGATGTCAAA ATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGGCCCCTTTCTCA GCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGAAGCCCTTGCCC TTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGGCCCTTAAGGAA TGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAAGGTTGGAAATA TTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTAAACATAGCCAG TTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCAGTTATTACTTA CGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCATTTGGACATCAT AAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAGTACCCCAAGCT GAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTATCAGGTTCCCCA ACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTTTATATGACAGT CACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGTTACAACACCCC CTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAGACAAAGTTGTT GAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCACATGCCCTTTA TTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACAGAAGTATTCAA GTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTAAAGGACAGAGC TAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCACATCAAAACTG ATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTTAAAGCACTAAT GACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGTAAAAAAAAGGG AGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCAAAAGCAAGAGC TCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCATTACTGAGTAT AGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGGAGACTCTCCAT TCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTACTTCCCACCCCC ACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGGGGTTGCTGATG GATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACCCGTCCCACTCC ATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCTGCAGAGATGGG GGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAAACCACCACGTG TCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCTGTAGTATTTAT TGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTTGAGGATGACGA AGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWILLTSTMASSTWESVHL*GSS*RMVHIMK MLVMVLVRASSPRLYLWRRQGRRR*QTG*SEPS GFPCWE*VVESCTLSSPCTQSCVPEAFPLLLRV HRQHPPLRAPQQSSATHRSFGNALGNCILDKDYL RSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARY NSCRPNMALGHPQLQQVTSPSRPSHAVIPADQD CSSRSLSSSAVESEATHQRKLRQKQLQQQFRERM EKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVS EPLLEKDFLAGVTQELISKYLEMLRLLKTTLKS GL*LPMQGMVWSSPRLEQTQPRPLTH*P*TTRW* PRTGLHTAFATRNHKQNLDLGTSKLIALTNAQQ ETGNLIGRART*RKGNMIHLN*GSGHIIGLCHK GTLENYFLVMKLFIAAGE*TSLRFILLHSEPYQE DLTESPLQLELSTFEKLVHHSSRERLWTDEYLF FGL*GFPLFITELLC**RWTF*ESPMAPCPQAI *ARLGPKHLPQQLSTFSCFA*RLPSFLYGPCL*C CPH*ALWITTGKRLEQCFSLLCINPGYNLHLI* IIYGFMTLQSCIVSRLTFFPKEGNNHLRPRKKA VFCFFFFFFF*DGVWLCCPDWSSVVQTQLSPQPL GPK*YSHLCLTKYRDYWCEPLCLARKGIFEKAN RIPY*QNRIYIYCLSEMLETRIVLHFLNICIHI MRPWGWDPSLNVEFTCVSCICLIHIILCMKQSFC IRRYTAAEEGWVFFSLRVAA*TVVCLVLCDLSH EVTCGIFHFWHHVSAQKFSDLRAFQLGMLKRNC FYFPISGVRCNPP*P*IGFS*CSDVS*FFPQNNE VCSLTTFFLRGGVWLCSAGWSAAVPS*LIAASA CQDSSNSPASGSRVAGITDTHHDARLIFVFLVE ETGFLHVGQAGLKLTSSDLPTSASQSKKY*NYRH EPLRPANLIIFLDVLFWAGLWLILKQ*LPIFWL RPIWEAVNLR*GSLKVLAGSRPFLFLSKLRRQQ RSSQELGIQL*IALNSK*CQ*ECEGIFWK*NPTA QQKQVWFI*MVSLNKSTKVTN*STKRNLTRSAT PRVSAKCTINGC*ETPKV*IFIFIFNSVHC*IM *GKHL*FRKFLMWKKILKVYY*HKNNLNSSVALV HSSRLVISYTGG*NQQ*ITPVLTFFVYE*KDVK IIIKPK*QMIMCCMRLHCSSGIKYVSLLGGPFL SYSAPCWFYPYSLHKKYKQRINKSF*SRGGSPCP FPFYLLKTFSSHLHI*GRRPTYLLTKGSRALKE *TGYRSLQPLSVVVVVVVVIPRICH*RG*KVGN IFSFPTKSQEAPQN*ES*LLSLSSEITKILNIAS *DSISGKL*K*LITTHYKEA*H*ITLIGCSYYL RGVLFI*KCEHISCTSRNKGGTIDYDPATIWTS *RK*VGISILS*ND*FRCGKIDRLTFHKM*YPKL RNAWYMCNKRKAIQTVFLRAIFSICDFSLSGSP T*KLAPHPLLFMFSFF*VTSVQA*LK*ERFI*Q SLARHHVCFLIL*SLSFTQHTPFF*VVQFCYNTP SSLFPKQNFLFLTTDANLTSHPTHLTPALDKVV E*LKKILFQFPFGITTAGLLGSHGPTISFHMPF IDLGSGKLMLMAEAIEVQGTKMSSKQHWETEVFK YSNLYNFTFTFIYLIQWKYKGKS*SVSISKGQS *LFLELFKRFIFYS*NYCCPTTFYISQCFHIKT DGKVTCPQGT*FFFFPNC*CCSQSC*YLLLKALM TYHKKELHPSTLVFFTGF*RAKKK*KREG*KKG RRAG*ERAQ*VVFVLWFGFLRE*GIQGMYQKQE LHPLEITDGFDFTLSSPASL*TRLS*PSSSLLSI DKQESMV*SCPLTKKLFFPSSPIPITGAGETLH SEGSMQY*KHSPLARNNQLNAALVVSI*ATSHP HPPPQHSISCPQIYSVNVSRSKWSAGWSLGGC*W ISANC*C*NSARRQFPQVGTGQVAVVLGHPSHS IERQMG*LESHTAVGGT*TW*SDLER*LMLQRW GQSHPGSRSWRCTVAQVQRTSLPFQGPENKTTTC QVWCGPWYKMCGANIYF*YNPVRYSRTVFCSIY WLFIFTK*G*K*SS*EGGMSCACVSVCV*LRMT K

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGWFIS* RCWLWC**GPQVQGFIFGEGKEGGGDRRAEASPQ ASLAGSKW*NPVLSLLRALSHVCRRPSHSYCEC TDSTLLSVLLSSPPLRTGVLGMHLETVFWTKTT *DH*ISFHASCLLKWI*LKRRDKILNRLWRRQDT TAADRTWPWDTHSCSR*PPLPDPAML*YRRTRT AAPEA*AHPPWRARPRTSGSSGRSSCSSSSGSG WRSSRFESPRRQLRRVRQRLRPLL*RTALL*LSQ NHSWRKTSLQE*LKN*SVNTLKCLDS*RQL*KV GCDSRCRGWCGQALV*SRPSPDL*HISLEQPDG DPEQDSTQRLPPETTSKIWILGPPNL*R*PTHNR KLGIS*EEPGHEEKEI*SILTEAQAT*LDSVTK GLWKTTFWS*NCSSLLENERHCDLSCFILNLIK RI*LRAHCS*S*ALLKSLSITLVGRGSGQMNTFS SACEASHYLLLNYYVNEDGHFRNHQWLLALKQY RPDLVLSTCLSNCLHSVVLHNVCLLSFTVHAFN VAHIKHCGSRQEKGWSSAFHYFVSIQATIFI*YK *FMDL*HYNPALFQD*HFFLRKEIII*DHEKRL FFVFFFFFFFETGSGCVALTGVQWCKHSSLHNL LGPSDTPTSALQNTGITGVSHCVWPEKAFLRKQI VYLINKIEYIYIAYLKCLKPELFCIF*IFVYT* *DLGDGTQV*TWNSPVFRVYASYT*FCA*NRVF V*EDTLQLKRAGFFFLLGSLHKLLYAWCFATCHT RSRVEFSTSGITSVLRNFLISEHFN*GCSNATV STSPFQV*DVTHLDHKLAFHSAQMFHNSSPKIM KFAV*QLFF*EGGSGSVAQAGVQRCHLDSLQPPP ARIQAILLPQAPE*LGLQTPTTTPD*FLYF**R RRGFSMLARLVSN*PQVICPPRPPKVKSTRIIG MSHCAQPI**FF*TYYFGLGCG*S*SSDFLFFGS DPFGKL*T*DKEV*KY*PAADRFYSFRS*GDSK GAPRN*GSSYKLHLTQNSASENVKESSGNETPQ LNKSKFGLFEWFH*ISLQR*QTEAQSAILQEAQH PELVQNVQLTAAEKPPKFEFLFLFLTVYIVK*C KENIYNSESF*CGKRYSKCTINTKII*TVQ*H* FIPLGLLFHTQEDEINSK*HQF*LFLCMSEKMSK LLLNPNNK**CAA*GSTAPLV*SMFHSWVAPFS ATQPLVGSILTLSTRNTSRE*INHFKAGVEALA LFLSTF*KPSALICIFRAVDQLTYLLREAGPLRN EQGTGASSLFLW*WWWWW*FPEYAIDVGKRLEI FSVSQLRVKKHPKIKSPSFFLCLQR*QRS*T*P VRTVFPESSENN**LPITRRHSTE*H*LVAVITY GEYYLFENVNILVVLLEIKVAQ*IMILQPFGHH KGNE*E*VFFPRMINSDVER*ID*LSTKCSTPS *EMHGTCATRGKQSKQFF*EQFSPSVTFHYQVPQ LEN*PPTPFSLCSVSSRSRLFKPSSNKKGLYDS HWPGITFVSSFFSLCLSPSTHHSFKLSNFVTTP PPHYSRNRTFCF*PLMPT*LPTLLILHLL*TKLL NDLKKYFFNFPLA*PLLDCWGHMDLRFPSTCPL LIWGLVS*C*WQKQ*KSKALKCPASNTGKQKYS STVIYTILHSHLFI*YNGNTKEKVKVSQ*VKDRA SFFWNFLNVSYFTLKTTVVQQHFIYHSVSTSKL MAKLHVRRELNFFFFLIANAAVKAANIFCLKH* *PIIRKSCTPVPWYFSLGSKEQRKNKKERGKKKG GGQGRREPSRWFLSCGSDS*GSEGFRACTKSKS STHSKLQMGLILH*VLQLPYEQDFPSPHPHY*V *INRKAWSEAAP*QKSCSFLPLPFQ*LERGRLSI LRAQCSIKNIPLWQEITS*MQH**LVYRLLPTP THPLSIPSPAPRSIQLMSLGLSGPQVGAWGVAD GFLQIADVEILQGGSSHRLELAKWLWCWATRPTP LSAKWGSWKAILLWGAHRLGEVT*RGS*CCRDG GRATQVRGHGDAQLPRCRELPCHSRALKTKPPR VKSGVGHGIKCVALTYTSSIIL*DTAEQSSVVFI GSLYLPSKGENNPHEREGCHVRVCLCVCS*G*R R

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHE DVGYGVSEGLKSKALSLEKARKEAVTDGLKRAL RLPLLGVSGRILYSLFSVHSVMCAGGLPTPTASA QTAPSSPCSSAVLRYAQEFWECTWKLYSGQRLP EITK*ASTPVAS*SGFN*SEETRS*TVCGGGKI QQLPTEHGPGTPTAAAGDLPFQTQPCCDTGGPGL QLPKPELIRRGERGHAPAEAPAEAAAAAVPGAD GEAAGSSLHAVS*EE*GSASGPSCDAQHSCNCL RTTPGERLPCRSDSRINQ*IP*NA*TLEDNSEKW AVTPDAGDGVVKPSSRADPAQTSDTLALNNQMV TQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTT GNWESHRKSQDMKKRKYDPS*LRLRPHNWTLSQR DFGKLLFGHEIVHRCWRMNVIAIYLASF*TLSR GSD*EPTAVRAEHF*KACPSL**GEALDR*IPF LRLVRLPTIYY*TIMLMKMDILGITNGSLPSSNI GQTWS*APASAIVYIQLFCITSAFFPLRSMPLM LPTLSTVDHDRKKVGAVLFTTLYQSRLQSSFNI NNLWIYDITILHCFKTDIFS*GRK*SSKTTKKGC FLFFFFFFFLRRGLAVLP*LEFSGANTALSTTS WAQVILPPLPYKIQGLLV*ATVSGQKRHF*ESK SYTLLTK*NIYILLI*NA*NQNCFAFFEYLYTHN ETLGMGPKSERGIHLCFVYMPHTHNFVHETEFL YKKIHCS*RGLGFFFS*GRCINCCMPGALRLVT RGHVWNFPLLASRQCSEIF*SQSISIRDAQTQLF LLPHFRCEM*PTLTINWLFIVLRCFIILPPK** SLQFNNFFFERGGLAL*RRLECSGAILTHCSLR LPGFKQFSCLRLPSSWDYRHPPRRPTNFCIFSRG DGVSPCWPGWSQTDLK*SAHLGLPK*KVLEL*A *AIAPSQFNNFFRRIILGWAVANLEAVTSYFLA QTHLGSCEPEIRKSESTSRQPTVSIPFEVKETAK ELPGIRDPVINCT*LKIVPVRM*RNLLEMKPHS STKASLVYLNGFIK*VYKGNKLKHKAQSYKKRN TQS*CKMYN*RLLRNPQSLNFYFYF*QCTLLNNV RKTFIIQKVFNVEKDTQSVLLTQK*FKQFSSTS SFL*ACYFIHRRMKSTVNNTSSDFFCV*VKRCQ NYY*TQITNDNVLHEAPLLLWYKVCFTLGWPLSQ LLSPLLVLSLLSPQEIQAENK*IILKQGWKPLP FSFLPFKNLQLSFAYLGP*TNLLTY*GKQGP*G MNRVQEPPASFCGSGGGGGDSQNMPLTWVKGWKY FQFPN*ESRSTPKLRVLASFFVFRDNKDPKHSQ LGQYFRKALKIINNYPLQGGIALNNTDWLQLLL TGSIIYLKM*TY*LYF*K*RWHNRL*SCNHLDII KEMSRNKYSFLE*LIQMWKDR*INFPQNVVPQA EKCMVHVQQEESNPNSFSESNFLHL*LFTIRFP NLKTSPPPPSLYVQFLLGHVCSSLAQIRKVYMTV TGQASRLFPHSLVFVFHPAHTILLSCPILLQHP LLTIPETELSVFNH*CQPNFPPYSSYTCSRQSC *MT*KNTFSISLWHNHCWTAGVTWTYDFLPHALY *SGVW*VDANGRSNRSPRH*NVQQATLGNRSIQ VQ*FIQFYIHIYLSNTMEIQRKKLKCLNK*RTE LAFFGTF*TFHILLLKLLLSNNILYITVFPHQN* WQSYMSAGNLIFFFS*LLMLQSKLLISFA*STN DLS*ERVAPQYPGIFHWVLKSKEKIKKRGVKKR EEGRVGESPVGGFCPVVRILEGVRDSGHVPKARA PPTRNYRWV*FYIEFSSFLMNKTFLALILITEY R*TGKHGLKLPLDKKAVLSFLSHSNNWSGGDSP F*GLNAVLKTFPSGKK*PVKCSTSS*YIGYFPPP PTPSAFHLLPPDLFS*CL*V*VVRRLEPGGLLM DFCKLLMLKFCKEAVPTGWNWPSGCGAGPPVPL H*APNGVAGKPYCCGGHIDLVK*PREVADAAEMG AEPPRFEVMEMHSCPGAENFLAIPGP*KQNHHV SSLVWAMV*NVWR*HILLV*SCKIQQNSLL*YL LALYIYQVRVKIILMRGRDVMCVCVCVCVVEDDE

Sequence RAD52.mAug05

TGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCAT GGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCACG CAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGAC CTGCTGCCCGGAAGGATCAGTGGGTGGTAGGAGAAGGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTAT CTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCAGGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTT CTCCGTGCACTCAGTCATGTGTGCCGGAGGCCTTCCCACTCCTACTGCGAGTGCACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTAC GCACAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGAT TTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGC AGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGGTATGC

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Single exon skipping

Frame 0

CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPSR SRMWILLTSTMASSTWESVHL*GSS*R*GDGVD LLPGRISGW*EKDGSYHEDVGYGVSEGLKSKAL SLEKARKEAVTDGLKRALRLPLLGVSGRILYSLF SVHSVMCAGGLPTPTASAQTAPSSPCSSAVLRY AQEFWECTWKLYSGQRLPEITK*ASTPVAS*SG FN*SEETRS*TVCGGGKIQQLPTEHGPGTPTAAA GDLPFQTQPCCDTGGPGLQLPVC

Frame 1

VMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGEGME*T CCPEGSVGGRRRMVHIMKMLVMVLVRASSPRLY LWRRQGRRR*QTG*SEPSGFPCWE*VVESCTLS SPCTQSCVPEAFPLLLRVHRQHPPLRAPQQSSAT HRSFGNALGNCILDKDYLRSLNKLPRQLPLEVD LTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQ QVTSPSRPSHAVIPADQDCSSRY

Frame 2

LCFGQCQYTAEEYQAIQKALRQRLGPEYISSRM AGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKVRGWSR PAARKDQWVVGEGWFIS*RCWLWC**GPQVQGFI FGEGKEGGGDRRAEASPQASLAGSKW*NPVLSL LRALSHVCRRPSHSYCECTDSTLLSVLLSSPPL RTGVLGMHLETVFWTKTT*DH*ISFHASCLLKWI *LKRRDKILNRLWRRQDTTAADRTWPWDTHSCS R*PPLPDPAML*YRRTRTAAPGM