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Sequence SYNE2.kAug05
CAGGGTCTCCCCCACTAAACCGTGCGGGTGTCAGGGGATAAAAGAAGTGCAAAAGCACAGGCTTGGAGGGGATGGCTTGTGTTAAGTTGCTTTGCTCCCATCAGAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCTGCCC
AAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGAGCTC
ATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGACAACC
TTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGCTCAC
AGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTACATTA
AATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAGGAAC
TCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCTCTGA
GAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCAT
CTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCT
CCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTG
TCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTA
TTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGT
CAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGC
CATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGA
CTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCA
CAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCA
GTGCCAGGACTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGAC
CCCCGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACA
GCCTTCTCATTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCA
AGATTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCA
GCTCCCCGAGCAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCC
TCTCAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTG
CACTCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACAC
CACCTGCCTGATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGG
CTTACCCAGCACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGC
AGAACTAGTTGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATA
ATGTAGGTATGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCC
TTAGCTGATGGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTC
TGTTCAGTACCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCA
ATCTGTATGAATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGA
TAGGGATGTTTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCG
GGGGGTTGGTACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
QGLPH*TVRVSGDKRSAKAQAWRGWLVLSCFAPIRIEETWRLWQKFLDDYSRFEDWLKSAERTAACP
NSSEVLYTSAKEELKRFEAFQRQIHERLTQLEL
INKQYRRLARENRTDTASRLKQMVHEGNQRWDN
LQRRVTAVLRRLRHFTNQREEFEGTRESILVWLT
EMDLQLTNVEHFSESDADDKMRQLNGFQQEITL
NTNKIDQLIVFGEQLIQKSEPLDAVLIEDELEE
LHRYCQEVFGRVSRFHRRLTSCTPGLEDEKEASE
NETDMEDPREIQTDSWRKRGESEEPSSPQSLCH
LVAPGHERSGCETPVSVDSIPLEWDHTGDVGGS
SSHEEDEEGPYYSALSGKSISDGHSWHVPDSPSC
PEHHYKQMEGDRNVPPVPPASSTPYKPPYGKLL
LPPGTDGGKEGPRVLNGNPQQEDGGLAGITEQQ
SGAFDRWEMIQAQELHNKLKIKQNLQQLNSDISA
ITTWLKKTEAELEMLKMAKPPSDIQEIELRVKR
LQEILKAFDTYKALVVSVNVSSKEFLQTESPES
TELQSRLRQLSLLWEAAQGAVDSWRGGLRQSLMQ
CQDFHQLSQNLLLWLASAKNRRQKAHVTDPKAD
PRALLECRRELMQLEKELVERQPQVDMLQEISN
SLLIKGHGEDCIEAEEKVHVIEKKLKQLREQVSQ
DLMALQGTQNPASPLPSFDEVDSGDQPPATSVP
APRAKQFRAVRTTEGEEETESRVPGSTRPQRSF
LSRVVRAALPLQLLLLLLLLLACLLPSSEEDYSC
TQANNFARSFYPMLRYTNGPPPT*RA*LATVLH
HLPDCQGCPARGPRPI*VT*CWQGPGTCADFFW
AYPARAPWSPGQLSDCVPPQEQGTCVAGAPGILA
ELVD*FRDLWKCQFPEEPSTL*ILVCL*NSIII
M*VWSMSSGVHHIYYRSKRGHSTLEMVQKLIGT
LS*WKQSIIFNTLRISFTPISWQF*AAGYTPKCS
VQYLALLFYIALNF*RNCIAWMWLFVHIF*QCP
ICMNNVNFDFFLKKLDFSWSF*LM*SKCQTTDL
IGMFL*VNFLRLFHIQSDALLWVLTVWPRRGWGR
GVGTET*SCL*YVQLRCFSLKNKISQTSVVL
Frame 1
RVSPTKPCGCQGIKEVQKHRLGGDGLC*VALLPSESRRRGACGRSF*TTILALRTGSSQLRGRQPA
QIPQRCCTRVPKRN*RGLRPFSGRFMSGSLSWSS
STSSTGGWPGRTAQTRPAG*SRWSTRATSAGTT
FRGGSQPSCGDSGISPTRGKNLRAPGRAFWCGS
QRWTCS*PTWSTSQRVTPMTRCAN*MASNRKLH*
IPTRLISSLCLGSS*FRRASPWMLC*LRMSWRN
STATARRCLEGSPGSTGGSPPALRAWKMKRRPL
RMKQTWKTPEKSRLILGVNGERARNRHLLSPCVI
*WPQGTSGLAARPLSAWTPSPWSGTTQATWGAP
PLTKRTRRAHTTAHCQVNPFRMATRGMFPTALP
VPSITTSKWKVTGMFHLFPLRPAPLINHPMESYY
YLQARMVAKKARES*MATHSRKTGDWPVSQSSS
QVPSTDGR*FKHRSFTISSK*NKICNS*TLISA
PSLLG*KKLKQSWKC*RWQSLPLISRK*N*E*RD
CRRY*KPLTLTRH*WSLST*AARNFCKPRAPNP
QSSKVDSAS*ACSGKQHRAQWTAGEGAYDSRSC
SARTSTS*VKICCCG*RVPRTGGRRLMSPIQRQT
PGLS*SVGGN*CNWKRSW*NVNLKWTCYRRFQT
AFSLRDMEKTVLKLKKRCMLLRRNSNSYGSKCP
KI*WPCREPRTQPHPCPASTR*TRGTSLLQHPCQ
LPEQSSSEQ*ELQKARRRQRAGSPAAHGHSAPS
SQGWSGQPYPCSCSSCCCCSWPACCPPPKKTTA
ALRPTTLPGPFTPC*GTPMGHPPHRGHSWPQCYT
TCLIAKGAQHVAPDQSE*LSVGKVPGPVQTSSG
LTQHGLPGAQGSFQIVFLPRSREPVWQVPRVFW
QN*LISLGISGNVSFLKSQALCEFWFVCKTALL*
CRYGQ*AVVSITYIIEASEDIPP*KWFRNS*AP
LADGNNQSYLIRLESVLLQSAGNFELPVIHQNV
LFST*LCSFILL*IFKEIVLHGCGYLCIFFNNAQ
SV*IM*TSIFF*KN*ILAGAFD*CKVNAKLPT*
*GCFCKLIF*DFFTSKVMLCFGF*LFGHGGGGG
GGLVQKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 2
GSPPLNRAGVRG*KKCKSTGLEGMACVKLLCSHQNRGDVAPVAEVFRRLFSL*GLAQVS*EDGSLP
KFLRGVVHECQRGTEEV*GLSAADS*AAHSAGA
HQQAVPAAGPGEPHRHGQQAEADGPRGQPALGQP
SEAGHSRPAETQAFHQPEGRI*GHQGEHSGVAH
RDGPAADQRGALLRE*RR*QDAPTEWLPTGNYI
KYQQD*SAHCVWGAADSEERAPGCCAD*G*AGGT
PPLLPGGVWKGLPVPPAAHLLHSGLGR*KGGL*
E*NRHGRPQRNPD*FLA*TGRERGTVISSVPVS
SSGPRARAVWLRDPCQRGLHPPGVGPHRRRGGLL
LSRRGRGGPILQRTVR*IHFGWPLVACSRQPFL
SRASLQANGR*QECSTCSPCVQHPL*TTLWKAT
ITSRHGWWQRRPASPEWQPTAGRRGTGRYHRAAV
RCLRQMGDDSSTGASQ*AQNKTKFATAEL*YQR
HHYLAEKN*SRAGNVKDGKASL*YPGNRTESEE
TAGDTESL*HLQGISGLCQREQQGISANREPRIH
RAPK*TPPAEPALGSSTGRSGQLERGLTTVAHA
VPGLPPVESKSAAVVSECQEPEAEGSCHRSKGR
PPGSPRVSEGTNATGKGAGRTSTSSGHVTGDFKQ
PSH*GTWRRLY*S*RKGACY*EETQTVTGASVP
RFNGLAGNPEPSLTPAQLRRGRLGGPASCNIRA
SSPSKAVQSSENYRRRGGDREQGPRQHTATALLP
LKGGPGSPTPAAAPPAAAAPGLPAALLRRRLQL
HSGQQLCPVLLPHAEVHQWATPHIEGIAGHSAT
PPA*LPRVPSTWPQTNLSDLVLARSRDLCRLLLG
LPSTGSLEPRAAFRLCSSPGAGNLCGRCPGYFG
RTS*LV*GSLEMSVS*RAKHFVNSGLFVKQHYY
NVGMVNEQWCPSHIL*KQARTFHPRNGSETHRHP
*LMETINHI*YA*NQFYSNQLAILSCRLYTKMF
CSVPSSALLYCFKFLKKLYCMDVVICAYFLTMP
NLYE*CKLRFFFKKIRF*LELLTNVK*MPNYRLD
RDVFVS*FSKTFSHPK*CFALGFNCLATAGVGA
GGWYRNLKLFVICTTQMFLIKKQN*PDFCCTE
Sequence SYNE2.iAug05
CTGTTATCTGGCTTTCAAAGTGATATGATTTAGTAATGCTACAAATTAAAAATACTGGTTTTTCCACTTAGCCTTGGAACTTTAAGCATGTCTTTTTCATGGAGGTCATCTAGGCTCTGTCTTTGCTTTGTGCAGAAAAGAATATTGATATAAGTATTATGGAATTTTAATGGACATTACAGAGATTTTAATGCGTCTCT
CATTGGAGTCACAGCTACTGAACACATCAGTTTTACTTACAAGTGTGATCCACTAGGATGAATGTCTTTGAACAGACTGTGAAAAGGCAAGGCCTTGCAA
ATAACAATTTACCCTGAGTGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGACGTCCTACTGCACGACTC
CGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTGCCATGTCCATGGAGCGG
CGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCT
GCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGA
GCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGAC
AACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGC
TCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTAC
ATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAG
GAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCT
CTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTG
TCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGC
TCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTT
CCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCT
ACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAG
CAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCA
GCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAA
GAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAA
TCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCA
TGCAGTGCCAGGACTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGC
AGACCCCCGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCA
AACAGCCTTCTCATTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGT
CCCAAGATTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGT
GCCAGCTCCCCGAGCAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCC
TTCCTCTCAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACA
GCTGCACTCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCT
ACACCACCTGCCTGATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTC
TGGGCTTACCCAGCACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTT
TGGCAGAACTAGTTGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTAT
TATAATGTAGGTATGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGC
ACCCTTAGCTGATGGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAAT
GTTCTGTTCAGTACCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATG
CCCAATCTGTATGAATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGAC
TTGATAGGGATGTTTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGG
GGCGGGGGGTTGGTACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
LLSGFQSDMI**CYKLKILVFPLSLGTLSMSFSWRSSRLCLCFVQKRILI*VLWNFNGHYRDFNASL
IGVTATEHISFTYKCDPLG*MSLNRL*KGKALQ
ITIYPEWIYSEILNNTAQGWSPCLTSVTSYCTT
PMPVQMRPSVTRSSRPPGAWTDAGGTFVPCPWSG
A*KSRRRGACGRSF*TTILALRTGSSQLRGRQP
AQIPQRCCTRVPKRN*RGLRPFSGRFMSGSLSW
SSSTSSTGGWPGRTAQTRPAG*SRWSTRATSAGT
TFRGGSQPSCGDSGISPTRGKNLRAPGRAFWCG
SQRWTCS*PTWSTSQRVTPMTRCAN*MASNRKL
H*IPTRLISSLCLGSS*FRRASPWMLC*LRMSWR
NSTATARRCLEGSPGSTGGSPPALRAWKMKRRP
LRMKQTWKTPEKSRLILGVNGERARNRHLLSPC
VI*WPQGTSGLAARPLSAWTPSPWSGTTQATWGA
PPLTKRTRRAHTTAHCQVNPFRMATRGMFPTAL
PVPSITTSKWKVTGMFHLFPLRPAPLINHPMES
YYYLQARMVAKKARES*MATHSRKTGDWPVSQSS
SQVPSTDGR*FKHRSFTISSK*NKICNS*TLIS
APSLLG*KKLKQSWKC*RWQSLPLISRK*N*E*
RDCRRY*KPLTLTRH*WSLST*AARNFCKPRAPN
PQSSKVDSAS*ACSGKQHRAQWTAGEGAYDSRS
CSARTSTS*VKICCCG*RVPRTGGRRLMSPIQR
QTPGLS*SVGGN*CNWKRSW*NVNLKWTCYRRFQ
TAFSLRDMEKTVLKLKKRCMLLRRNSNSYGSKC
PKI*WPCREPRTQPHPCPASTR*TRGTSLLQHP
CQLPEQSSSEQ*ELQKARRRQRAGSPAAHGHSAP
SSQGWSGQPYPCSCSSCCCCSWPACCPPPKKTT
AALRPTTLPGPFTPC*GTPMGHPPHRGHSWPQC
YTTCLIAKGAQHVAPDQSE*LSVGKVPGPVQTSS
GLTQHGLPGAQGSFQIVFLPRSREPVWQVPRVF
WQN*LISLGISGNVSFLKSQALCEFWFVCKTAL
L*CRYGQ*AVVSITYIIEASEDIPP*KWFRNS*A
PLADGNNQSYLIRLESVLLQSAGNFELPVIHQN
VLFST*LCSFILL*IFKEIVLHGCGYLCIFFNN
AQSV*IM*TSIFF*KN*ILAGAFD*CKVNAKLPT
**GCFCKLIF*DFFTSKVMLCFGF*LFGHGGGG
GGGLVQKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 1
CYLAFKVI*FSNATN*KYWFFHLALEL*ACLFHGGHLGSVFALCRKEY*YKYYGILMDITEILMRL
SLESQLLNTSVLLTSVIH*DECL*TDCEKARPCK
*QFTLSGSTARY*TTQRRGGVRV*HL*RPTARL
RCLCK*DRV*LDPADHQEPGQTLEEHLCHVHGA
AHENRGDVAPVAEVFRRLFSL*GLAQVS*EDGSL
PKFLRGVVHECQRGTEEV*GLSAADS*AAHSAG
AHQQAVPAAGPGEPHRHGQQAEADGPRGQPALG
QPSEAGHSRPAETQAFHQPEGRI*GHQGEHSGVA
HRDGPAADQRGALLRE*RR*QDAPTEWLPTGNY
IKYQQD*SAHCVWGAADSEERAPGCCAD*G*AG
GTPPLLPGGVWKGLPVPPAAHLLHSGLGR*KGGL
*E*NRHGRPQRNPD*FLA*TGRERGTVISSVPV
SSSGPRARAVWLRDPCQRGLHPPGVGPHRRRGG
LLLSRRGRGGPILQRTVR*IHFGWPLVACSRQPF
LSRASLQANGR*QECSTCSPCVQHPL*TTLWKA
TITSRHGWWQRRPASPEWQPTAGRRGTGRYHRA
AVRCLRQMGDDSSTGASQ*AQNKTKFATAEL*YQ
RHHYLAEKN*SRAGNVKDGKASL*YPGNRTESE
ETAGDTESL*HLQGISGLCQREQQGISANREPR
IHRAPK*TPPAEPALGSSTGRSGQLERGLTTVAH
AVPGLPPVESKSAAVVSECQEPEAEGSCHRSKG
RPPGSPRVSEGTNATGKGAGRTSTSSGHVTGDF
KQPSH*GTWRRLY*S*RKGACY*EETQTVTGASV
PRFNGLAGNPEPSLTPAQLRRGRLGGPASCNIR
ASSPSKAVQSSENYRRRGGDREQGPRQHTATAL
LPLKGGPGSPTPAAAPPAAAAPGLPAALLRRRLQ
LHSGQQLCPVLLPHAEVHQWATPHIEGIAGHSA
TPPA*LPRVPSTWPQTNLSDLVLARSRDLCRLL
LGLPSTGSLEPRAAFRLCSSPGAGNLCGRCPGYF
GRTS*LV*GSLEMSVS*RAKHFVNSGLFVKQHY
YNVGMVNEQWCPSHIL*KQARTFHPRNGSETHR
HP*LMETINHI*YA*NQFYSNQLAILSCRLYTKM
FCSVPSSALLYCFKFLKKLYCMDVVICAYFLTM
PNLYE*CKLRFFFKKIRF*LELLTNVK*MPNYR
LDRDVFVS*FSKTFSHPK*CFALGFNCLATAGVG
AGGWYRNLKLFVICTTQMFLIKKQN*PDFCCTE
Frame 2
VIWLSK*YDLVMLQIKNTGFST*PWNFKHVFFMEVI*ALSLLCAEKNIDISIMEF*WTLQRF*CVS
HWSHSY*THQFYLQV*STRMNVFEQTVKRQGLA
NNNLP*VDLQRDIEQHSAGVESVFNICDVLLHDS
DACANETECDSIQQTTRSLDRRWRNICAMSMER
RMKIEETWRLWQKFLDDYSRFEDWLKSAERTAA
CPNSSEVLYTSAKEELKRFEAFQRQIHERLTQLE
LINKQYRRLARENRTDTASRLKQMVHEGNQRWD
NLQRRVTAVLRRLRHFTNQREEFEGTRESILVW
LTEMDLQLTNVEHFSESDADDKMRQLNGFQQEIT
LNTNKIDQLIVFGEQLIQKSEPLDAVLIEDELE
ELHRYCQEVFGRVSRFHRRLTSCTPGLEDEKEA
SENETDMEDPREIQTDSWRKRGESEEPSSPQSLC
HLVAPGHERSGCETPVSVDSIPLEWDHTGDVGG
SSSHEEDEEGPYYSALSGKSISDGHSWHVPDSP
SCPEHHYKQMEGDRNVPPVPPASSTPYKPPYGKL
LLPPGTDGGKEGPRVLNGNPQQEDGGLAGITEQ
QSGAFDRWEMIQAQELHNKLKIKQNLQQLNSDI
SAITTWLKKTEAELEMLKMAKPPSDIQEIELRVK
RLQEILKAFDTYKALVVSVNVSSKEFLQTESPE
STELQSRLRQLSLLWEAAQGAVDSWRGGLRQSL
MQCQDFHQLSQNLLLWLASAKNRRQKAHVTDPKA
DPRALLECRRELMQLEKELVERQPQVDMLQEIS
NSLLIKGHGEDCIEAEEKVHVIEKKLKQLREQV
SQDLMALQGTQNPASPLPSFDEVDSGDQPPATSV
PAPRAKQFRAVRTTEGEEETESRVPGSTRPQRS
FLSRVVRAALPLQLLLLLLLLLACLLPSSEEDY
SCTQANNFARSFYPMLRYTNGPPPT*RA*LATVL
HHLPDCQGCPARGPRPI*VT*CWQGPGTCADFF
WAYPARAPWSPGQLSDCVPPQEQGTCVAGAPGI
LAELVD*FRDLWKCQFPEEPSTL*ILVCL*NSII
IM*VWSMSSGVHHIYYRSKRGHSTLEMVQKLIG
TLS*WKQSIIFNTLRISFTPISWQF*AAGYTPK
CSVQYLALLFYIALNF*RNCIAWMWLFVHIF*QC
PICMNNVNFDFFLKKLDFSWSF*LM*SKCQTTD
LIGMFL*VNFLRLFHIQSDALLWVLTVWPRRGW
GRGVGTET*SCL*YVQLRCFSLKNKISQTSVVL
Sequence SYNE2.hAug05
GGCCCAGAGTCTGCTCCAGCTCTGGAAGGCCTATAGCAATGCTCATGGTGAAGCTGCCGCAAGGCTGAAGCAGCAGGAAGCAAAGTTTCAACAGCTCGCAAACATCAGCATGTCTGGAAACAACCTGGCAGAGATCCTGCCCCCAGCCCTGCAGGACATAAAGGAGCTGCAGCATGATGTGCAGAAAACAAAAGAAGCCT
TTCTCCAAAATTCCAGTGTCCTGGATCGACTCCCACAACCCGCAGAGTCCAGCACCCACATGCTCCTCCCGGGCCCCCTGCACTCTCTCCAGAGGGCTGC
TTATTTGGAAAAGATGCTGCTTGTGAAAGCAAATGAATTTGAGTTTGTTCTCTCACAGTTTAAGGATTTTGGAGTCCGGCTGGAATCTTTAAAAGGTCTT
ATTATGCATGAAGAAGAGAATTTGGATAGACTTCACCAACAGGAAAAAGAAAATCCTGACTCATTCCTGAATCATGTGCTGGCACTGACAGCCCAATCAC
CTGATATTGAACATTTGAATGAAGTGAGCCTCAAGCTCCCACTTAGTGACGTAGCTGTGAAGACGTTACAAAATATGAACCGGCAATGGATTCGGGCCAC
GGCCACGGCACTGGAGCGCTGCAGTGAGCTTCAGGGAATTGGATTGAATGAAAAGTTTCTTTATTGCTGTGAAAAGTGGATCCAACTTTTGGAGAAGATA
GAAGAAGCACTCAAAGTGGATGTGGCTAACAGCCTTCCTGAGCTCCTGGAGCAGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGA
CAATTGCTGATTCCTATGTCACCCAGTCCTTACAACTCCTGGACACAACAGAAATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGA
TCGGTGGCAGAATGCTGTCCAGGGTGTTCGGCAGAGGAAGGGTGACGTTGATGGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTG
TTTCGCTTCCTCACTGACACCAGCCACCTGCTATCTGCAGTGAAGGGCCAGGAGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGA
ATAAAGAAATTCATTTTCAAAGGAGGCGAACTACCTGTGCCCTAACCTTGGAAGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTC
TGTGGGTAGGAGAATCAGTCAACTTCAGGACAGCTGGAAAGACATGGAGCCCCAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGG
GACCAGTGTGAAAAGAAAATCAAGGAGTTGAAAAGCAGGCTGCAAGTTTTAAAGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATA
ACGAAAAAGAGCTGATTAAGGAACTAGAACAGTCTTTGGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGT
TCTCGTGGAAGATGTGATGGTTTTGAAGGAGCAAATAGAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATT
GAAGACAGACTCAATACATGGGTTGTATTCAATGAAAAAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACA
TTAGTATTGAAGAAATGATTGAAAAGTTACAGAAGGGTGAAGAAGCTGAAGGAGACCTTTGCTTTTATTCAGCAGTTGGACAAAAACATGAGCAACCTTC
GCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGATGATCAAGAGATCCAGAAGAGGCTCGCTGAGCAGCAGGA
TCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGACGTCCTACTGCACGACTCCGATGCCTGTGCAAATGAGACC
GAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTGCCATGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGT
GGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTT
GTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGG
CGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAG
CCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCT
GACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTACATTAAATACCAACAAGATTGAT
CAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGG
AGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGA
AGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCAC
GAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACG
AGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAA
GCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTACCTCCAGGCACGGAT
GGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGAT
GGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCATCACTACTTGGCTGAA
AAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAA
GCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGAC
TCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTGCCAGGACTTCCACCA
GTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCCCGGGCTCTCCTAGAG
TGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCATTAAGGGAC
ATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGATTTAATGGCCTTGCA
GGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGAGCAAAGCAG
TTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCTCAAGGGTGGTCCGGG
CAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGCCAACAACTT
TGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCACCTGCCTGATTGCCAA
GGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTTACCCAGCACGGGCTC
CCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGAACTAGTTGATTAGTT
TAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATGTAGGTATGGTCAATG
AGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTAGCTGATGGAAACAAT
CAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGTTCAGTACCTAGCTCT
GCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATCTGTATGAATAATGTA
AACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAGGGATGTTTTTGTAAG
TTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGGGGTTGGTACAGAAAC
TTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
GPESAPALEGL*QCSW*SCRKAEAAGSKVSTARKHQHVWKQPGRDPAPSPAGHKGAAA*CAENKRSL
SPKFQCPGSTPTTRRVQHPHAPPGPPALSPEGC
LFGKDAACESK*I*VCSLTV*GFWSPAGIFKRS
YYA*RREFG*TSPTGKRKS*LIPESCAGTDSPIT
*Y*TFE*SEPQAPT**RSCEDVTKYEPAMDSGH
GHGTGALQ*ASGNWIE*KVSLLL*KVDPTFGED
RRSTQSGCG*QPS*APGAAENL*DVRS*SFYKPD
NC*FLCHPVLTTPGHNRNREQTRIYYRILKADG
SVAECCPGCSAEEG*R*WAGEAVARFHYFCGEL
VSLPH*HQPPAICSEGPGALQPLPNQKSDP*AEE
*RNSFSKEANYLCPNLGSWRKVTAHN*PEN*RV
CG*ENQSTSGQLERHGAPAGRDD*AVPEHCRDL
GPV*KENQGVEKQAASFKGTK*RSSSRASRGPP*
RKRAD*GTRTVFG*LDSELERTSNYEGGLNPAR
SRGRCDGFEGANRAFAQTMGGPLLKGGHT*TGD
*RQTQYMGCIQ*KK*RVVCLAGADGKQSSTDSGH
*Y*RND*KVTEG*RS*RRPLLLFSSWTKT*ATF
APGWLELSLSFPSLLFMMSAMIKRSRRGSLSSR
IYSEILNNTAQGWSPCLTSVTSYCTTPMPVQMRP
SVTRSSRPPGAWTDAGGTFVPCPWSGA*KSRRR
GACGRSF*TTILALRTGSSQLRGRQPAQIPQRC
CTRVPKRN*RGLRPFSGRFMSGSLSWSSSTSSTG
GWPGRTAQTRPAG*SRWSTRATSAGTTFRGGSQ
PSCGDSGISPTRGKNLRAPGRAFWCGSQRWTCS
*PTWSTSQRVTPMTRCAN*MASNRKLH*IPTRLI
SSLCLGSS*FRRASPWMLC*LRMSWRNSTATAR
RCLEGSPGSTGGSPPALRAWKMKRRPLRMKQTW
KTPEKSRLILGVNGERARNRHLLSPCVI*WPQGT
SGLAARPLSAWTPSPWSGTTQATWGAPPLTKRT
RRAHTTAHCQVNPFRMATRGMFPTALPVPSITT
SKWKVTGMFHLFPLRPAPLINHPMESYYYLQARM
VAKKARES*MATHSRKTGDWPVSQSSSQVPSTD
GR*FKHRSFTISSK*NKICNS*TLISAPSLLG*
KKLKQSWKC*RWQSLPLISRK*N*E*RDCRRY*K
PLTLTRH*WSLST*AARNFCKPRAPNPQSSKVD
SAS*ACSGKQHRAQWTAGEGAYDSRSCSARTST
S*VKICCCG*RVPRTGGRRLMSPIQRQTPGLS*S
VGGN*CNWKRSW*NVNLKWTCYRRFQTAFSLRD
MEKTVLKLKKRCMLLRRNSNSYGSKCPKI*WPC
REPRTQPHPCPASTR*TRGTSLLQHPCQLPEQSS
SEQ*ELQKARRRQRAGSPAAHGHSAPSSQGWSG
QPYPCSCSSCCCCSWPACCPPPKKTTAALRPTT
LPGPFTPC*GTPMGHPPHRGHSWPQCYTTCLIAK
GAQHVAPDQSE*LSVGKVPGPVQTSSGLTQHGL
PGAQGSFQIVFLPRSREPVWQVPRVFWQN*LIS
LGISGNVSFLKSQALCEFWFVCKTALL*CRYGQ*
AVVSITYIIEASEDIPP*KWFRNS*APLADGNN
QSYLIRLESVLLQSAGNFELPVIHQNVLFST*L
CSFILL*IFKEIVLHGCGYLCIFFNNAQSV*IM*
TSIFF*KN*ILAGAFD*CKVNAKLPT**GCFCK
LIF*DFFTSKVMLCFGF*LFGHGGGGGGGLVQK
LEAVCDMYNSDVSH*KTKLARLLLY*
Frame 1
AQSLLQLWKAYSNAHGEAAARLKQQEAKFQQLANISMSGNNLAEILPPALQDIKELQHDVQKTKEA
FLQNSSVLDRLPQPAESSTHMLLPGPLHSLQRAA
YLEKMLLVKANEFEFVLSQFKDFGVRLESLKGL
IMHEEENLDRLHQQEKENPDSFLNHVLALTAQS
PDIEHLNEVSLKLPLSDVAVKTLQNMNRQWIRAT
ATALERCSELQGIGLNEKFLYCCEKWIQLLEKI
EEALKVDVANSLPELLEQQKTYKMLEAEVSINQ
TIADSYVTQSLQLLDTTEIENRPEFITEFSKLTD
RWQNAVQGVRQRKGDVDGLVRQWQDFTTSVENL
FRFLTDTSHLLSAVKGQERFSLYQTRSLIHELK
NKEIHFQRRRTTCALTLEAGEKLLLTTDLKTKES
VGRRISQLQDSWKDMEPQLAEMIKQFQSTVETW
DQCEKKIKELKSRLQVLKAQSEDPLPELHEDLH
NEKELIKELEQSLASWTQNLKELQTMKADLTRHV
LVEDVMVLKEQIEHLHRQWEDLCLRVAIRKQEI
EDRLNTWVVFNEKNKELCAWLVQMENKVLQTAD
ISIEEMIEKLQKGEEAEGDLCFYSAVGQKHEQPS
HLVGSN*V*AFQACCL*CLR*SRDPEEAR*AAG
STARY*TTQRRGGVRV*HL*RPTARLRCLCK*D
RV*LDPADHQEPGQTLEEHLCHVHGAAHENRGDV
APVAEVFRRLFSL*GLAQVS*EDGSLPKFLRGV
VHECQRGTEEV*GLSAADS*AAHSAGAHQQAVP
AAGPGEPHRHGQQAEADGPRGQPALGQPSEAGHS
RPAETQAFHQPEGRI*GHQGEHSGVAHRDGPAA
DQRGALLRE*RR*QDAPTEWLPTGNYIKYQQD*
SAHCVWGAADSEERAPGCCAD*G*AGGTPPLLPG
GVWKGLPVPPAAHLLHSGLGR*KGGL*E*NRHG
RPQRNPD*FLA*TGRERGTVISSVPVSSSGPRA
RAVWLRDPCQRGLHPPGVGPHRRRGGLLLSRRGR
GGPILQRTVR*IHFGWPLVACSRQPFLSRASLQ
ANGR*QECSTCSPCVQHPL*TTLWKATITSRHG
WWQRRPASPEWQPTAGRRGTGRYHRAAVRCLRQM
GDDSSTGASQ*AQNKTKFATAEL*YQRHHYLAE
KN*SRAGNVKDGKASL*YPGNRTESEETAGDTE
SL*HLQGISGLCQREQQGISANREPRIHRAPK*T
PPAEPALGSSTGRSGQLERGLTTVAHAVPGLPP
VESKSAAVVSECQEPEAEGSCHRSKGRPPGSPR
VSEGTNATGKGAGRTSTSSGHVTGDFKQPSH*GT
WRRLY*S*RKGACY*EETQTVTGASVPRFNGLA
GNPEPSLTPAQLRRGRLGGPASCNIRASSPSKA
VQSSENYRRRGGDREQGPRQHTATALLPLKGGPG
SPTPAAAPPAAAAPGLPAALLRRRLQLHSGQQL
CPVLLPHAEVHQWATPHIEGIAGHSATPPA*LP
RVPSTWPQTNLSDLVLARSRDLCRLLLGLPSTGS
LEPRAAFRLCSSPGAGNLCGRCPGYFGRTS*LV
*GSLEMSVS*RAKHFVNSGLFVKQHYYNVGMVN
EQWCPSHIL*KQARTFHPRNGSETHRHP*LMETI
NHI*YA*NQFYSNQLAILSCRLYTKMFCSVPSS
ALLYCFKFLKKLYCMDVVICAYFLTMPNLYE*C
KLRFFFKKIRF*LELLTNVK*MPNYRLDRDVFVS
*FSKTFSHPK*CFALGFNCLATAGVGAGGWYRN
LKLFVICTTQMFLIKKQN*PDFCCTE
Frame 2
PRVCSSSGRPIAMLMVKLPQG*SSRKQSFNSSQTSACLETTWQRSCPQPCRT*RSCSMMCRKQKKP
FSKIPVSWIDSHNPQSPAPTCSSRAPCTLSRGL
LIWKRCCL*KQMNLSLFSHSLRILESGWNL*KVL
LCMKKRIWIDFTNRKKKILTHS*IMCWH*QPNH
LILNI*MK*ASSSHLVT*L*RRYKI*TGNGFGP
RPRHWSAAVSFRELD*MKSFFIAVKSGSNFWRR*
KKHSKWMWLTAFLSSWSSRKPIRC*KLKFL*TR
QLLIPMSPSPYNSWTQQK*RTDQNLLQNSQS*R
IGGRMLSRVFGRGRVTLMGW*GSGKISLLLWRTC
FASSLTPATCYLQ*RARSASASTKPEV*SMS*R
IKKFIFKGGELPVP*PWKLEKSYCSQLT*KLKS
LWVGESVNFRTAGKTWSPSWQR*LSSSRAL*RPG
TSVKRKSRS*KAGCKF*RHKVKILFQSFTRTSI
TKKS*LRN*NSLWLAGLRT*KNFKL*RRT*PGT
FSWKM*WF*RSK*SICTDNGRTSA*GWPYVNRRL
KTDSIHGLYSMKKIKSCVPGWCRWKTKFYRQRT
LVLKK*LKSYRRVKKLKETFAFIQQLDKNMSNL
RTWLARIESELSKPVVYDVCDDQEIQKRLAEQQD
LQRDIEQHSAGVESVFNICDVLLHDSDACANET
ECDSIQQTTRSLDRRWRNICAMSMERRMKIEET
WRLWQKFLDDYSRFEDWLKSAERTAACPNSSEVL
YTSAKEELKRFEAFQRQIHERLTQLELINKQYR
RLARENRTDTASRLKQMVHEGNQRWDNLQRRVT
AVLRRLRHFTNQREEFEGTRESILVWLTEMDLQL
TNVEHFSESDADDKMRQLNGFQQEITLNTNKID
QLIVFGEQLIQKSEPLDAVLIEDELEELHRYCQ
EVFGRVSRFHRRLTSCTPGLEDEKEASENETDME
DPREIQTDSWRKRGESEEPSSPQSLCHLVAPGH
ERSGCETPVSVDSIPLEWDHTGDVGGSSSHEED
EEGPYYSALSGKSISDGHSWHVPDSPSCPEHHYK
QMEGDRNVPPVPPASSTPYKPPYGKLLLPPGTD
GGKEGPRVLNGNPQQEDGGLAGITEQQSGAFDR
WEMIQAQELHNKLKIKQNLQQLNSDISAITTWLK
KTEAELEMLKMAKPPSDIQEIELRVKRLQEILK
AFDTYKALVVSVNVSSKEFLQTESPESTELQSR
LRQLSLLWEAAQGAVDSWRGGLRQSLMQCQDFHQ
LSQNLLLWLASAKNRRQKAHVTDPKADPRALLE
CRRELMQLEKELVERQPQVDMLQEISNSLLIKG
HGEDCIEAEEKVHVIEKKLKQLREQVSQDLMALQ
GTQNPASPLPSFDEVDSGDQPPATSVPAPRAKQ
FRAVRTTEGEEETESRVPGSTRPQRSFLSRVVR
AALPLQLLLLLLLLLACLLPSSEEDYSCTQANNF
ARSFYPMLRYTNGPPPT*RA*LATVLHHLPDCQ
GCPARGPRPI*VT*CWQGPGTCADFFWAYPARA
PWSPGQLSDCVPPQEQGTCVAGAPGILAELVD*F
RDLWKCQFPEEPSTL*ILVCL*NSIIIM*VWSM
SSGVHHIYYRSKRGHSTLEMVQKLIGTLS*WKQ
SIIFNTLRISFTPISWQF*AAGYTPKCSVQYLAL
LFYIALNF*RNCIAWMWLFVHIF*QCPICMNNV
NFDFFLKKLDFSWSF*LM*SKCQTTDLIGMFL*
VNFLRLFHIQSDALLWVLTVWPRRGWGRGVGTET
*SCL*YVQLRCFSLKNKISQTSVVL
Sequence SYNE2.eAug05
CACTGAGAGCTTCTTTAGCACAGTTTGAACAAAAATGGACAATGCTCATAACTCAACTTCCAGATATTCAAGAAAAACTTCACCAGCTTCAAATGGAGAAATTGCCGTCTCGTAAAGCAATCACAGAAATGATTAGCTGGATGAACAATGTGGAGCATCAAACTTCAGATGAAGACTCCGTGCATTCACCAAGTTCTGCA
TCTCAAGTTAAACATCTTCTTCAGAAGCACAAGGAGTTTAGAATGGAAATGGACTATAAACAGTGGATAGTTGACTTCGTTAACCAGTCATTACTTCAGC
TAAGCACCTGTGATGTAGAAAGCAAGCGCTATGAAAGAACGGAGTTTGCAGAGCACCTGGGGGAGATGAACCGCCAGTGGCACCGTGTACATGGAATGCT
GAATAGAAAGATACAACATTTAGAACAACTTCTAGAAAGTATCACTGAGAGTGAAAATAAAATACAGATCTTGAACAACTGGCTGGAAGCACAAGAAGAG
AGACTGAAAACTTTACAAAAACCTGAAAGTGTGATCTCAGTGCAGAAGCTGCTCCTGGACTGTCAGGATATAGAAAATCAACTTGCAATTAAATCCAAAG
CACTAGATGAGTTGAAACAAAGTTATCTGACTTTGGAGAGTGGGGCAGTGCCATTGTTAGAAGATACAGCATCCCGAATTGATGAGTTATTTCAAAAGAG
AAGCAGTGTTCTCACTCAGGTCAATCAGCTCAAAACCTCCATGCAGTCAGTTTTACAGGAGTGGAAGATTTATGATCAACTCTATGATGAAGTGAATATG
ATGACAATCCGATTCTGGTACTGCATGGAACACAGCAAGCCTGTGGTGTTATCATTGGAGACCTTGAGATGCCAGGTGGAGAACCTTCAGTCTCTGCAAG
ATGAAGCTGAGAGCAGTGAAGGGAGTTGGGAGAAACTCCAGGAGGTTATCGGCAAACTCAAAGGTCTCTGCCCCTCTGTTGCTGAAATAATCGAAGAGAA
ATGCCAAAATACTCATAAAAGGTGGACTCAGGTGAACCAAGCCATTGCAGACCAGTTGCAGAAGGCCCAGAGTCTGCTCCAGCTCTGGAAGGCCTATAGC
AATGCTCATGGTGAAGCTGCCGCAAGGCTGAAGCAGCAGGAAGCAAAGTTTCAACAGCTCGCAAACATCAGCATGTCTGGAAACAACCTGGCAGAGATCC
TGCCCCCAGCCCTGCAGGACATAAAGGAGCTGCAGCATGATGTGCAGAAAACAAAAGAAGCCTTTCTCCAAAATTCCAGTGTCCTGGATCGACTCCCACA
ACCCGCAGAGTCCAGCACCCACATGCTCCTCCCGGGCCCCCTGCACTCTCTCCAGAGGGCTGCTTATTTGGAAAAGATGCTGCTTGTGAAAGCAAATGAA
TTTGAGTTTGTTCTCTCACAGTTTAAGGATTTTGGAGTCCGGCTGGAATCTTTAAAAGGTCTTATTATGCATGAAGAAGAGAATTTGGATAGACTTCACC
AACAGGAAAAAGAAAATCCTGACTCATTCCTGAATCATGTGCTGGCACTGACAGCCCAATCACCTGATATTGAACATTTGAATGAAGTGAGCCTCAAGCT
CCCACTTAGTGACGTAGCTGTGAAGACGTTACAAAATATGAACCGGCAATGGATTCGGGCCACGGCCACGGCACTGGAGCGCTGCAGTGAGCTTCAGGGA
ATTGGATTGAATGAAAAGTTTCTTTATTGCTGTGAAAAGTGGATCCAACTTTTGGAGAAGATAGAAGAAGCACTCAAAGTGGATGTGGCTAACAGCCTTC
CTGAGCTCCTGGAGCAGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGACAATTGCTGATTCCTATGTCACCCAGTCCTTACAACT
CCTGGACACAACAGAAATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGATCGGTGGCAGAATGCTGTCCAGGGTGTTCGGCAGAGG
AAGGGTGACGTTGATGGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTGTTTCGCTTCCTCACTGACACCAGCCACCTGCTATCTG
CAGTGAAGGGCCAGGAGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGAATAAAGAAATTCATTTTCAAAGGAGGCGAACTACCTG
TGCCCTAACCTTGGAAGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTCTGTGGGTAGGAGAATCAGTCAACTTCAGGACAGCTGG
AAAGACATGGAGCCCCAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGGGACCAGTGTGAAAAGAAAATCAAGGAGTTGAAAAGCA
GGCTGCAAGTTTTAAAGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATAACGAAAAAGAGCTGATTAAGGAACTAGAACAGTCTTT
GGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGTTCTCGTGGAAGATGTGATGGTTTTGAAGGAGCAAATA
GAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATTGAAGACAGACTCAATACATGGGTTGTATTCAATGAAA
AAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACATTAGTATTGAAGAAATGATTGAAAAGTTACAGAAGGA
CTGCATGGAAGAAATAAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCAGTTGATCAAGGCCAGCAACAAATCAAGAGCAGCTGAG
ATCGATGACAAGCTCAACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCAAGGGTGAAGAAGCTGAAGGAGACCTTTGCTTTTATTC
AGCAGTTGGACAAAAACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGATGATCAAGA
GATCCAGAAGAGGCTCGCTGAGCAGCAGCCCACCCATGGGGTACAGCAGAAATATTATCTGATGATGACTAAAAATGCCATGTTTATTCGTGAAGAGGTT
TTCCAGTTTTTTCCTATGACCATGCATTTTCTCTTCATAAATGTTATTTTTCCCAAATTGGGGAACTGCATCACTATCATCATCAAAGGACAAGACTCCA
GAGACCCCACCTCTCTCCAAGCCACCACAGCTTTGGCTGGACTTTATCAGTTGGGGAGGCAAGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGG
TGGAGTCCGTGTTTAACATCTGTGACGTCCTACTGCACGACTCCGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCT
GGACAGACGCTGGAGGAACATTTGTGCCATGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCT
CGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTG
AGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAG
CAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAG
AGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCG
ATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTACATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAA
GAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGG
CTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTA
AACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGA
CTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAA
TCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTC
CCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAA
CCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAG
CTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGG
CAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGT
CAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAG
GGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTGCCAGGGCTCTAAGACACGGCCGCGCTCTGATGTGTTGTTTTTTAAGGACT
TCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCCCGGGCTCT
CCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCATT
AAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGATTTAATGG
CCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGAGC
AAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCTCAAGGGTG
GTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGCCA
ACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCACCTGCCTGA
TTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTTACCCAGCA
CGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGAACTAGTTG
ATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATGTAGGTATG
GTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTAGCTGATGG
AAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGTTCAGTACC
TAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATCTGTATGAA
TAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAGGGATGTTT
TTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGGGGTTGGTA
CAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
H*ELL*HSLNKNGQCS*LNFQIFKKNFTSFKWRNCRLVKQSQK*LAG*TMWSIKLQMKTPCIHQVLH
LKLNIFFRSTRSLEWKWTINSG*LTSLTSHYFS
*APVM*KASAMKERSLQSTWGR*TASGTVYMEC
*IERYNI*NNF*KVSLRVKIKYRS*TTGWKHKKR
D*KLYKNLKV*SQCRSCSWTVRI*KINLQLNPK
H*MS*NKVI*LWRVGQCHC*KIQHPELMSYFKR
EAVFSLRSISSKPPCSQFYRSGRFMINSMMK*I*
*QSDSGTAWNTASLWCYHWRP*DARWRTFSLCK
MKLRAVKGVGRNSRRLSANSKVSAPLLLK*SKR
NAKILIKGGLR*TKPLQTSCRRPRVCSSSGRPIA
MLMVKLPQG*SSRKQSFNSSQTSACLETTWQRS
CPQPCRT*RSCSMMCRKQKKPFSKIPVSWIDSH
NPQSPAPTCSSRAPCTLSRGLLIWKRCCL*KQMN
LSLFSHSLRILESGWNL*KVLLCMKKRIWIDFT
NRKKKILTHS*IMCWH*QPNHLILNI*MK*ASS
SHLVT*L*RRYKI*TGNGFGPRPRHWSAAVSFRE
LD*MKSFFIAVKSGSNFWRR*KKHSKWMWLTAF
LSSWSSRKPIRC*KLKFL*TRQLLIPMSPSPYN
SWTQQK*RTDQNLLQNSQS*RIGGRMLSRVFGRG
RVTLMGW*GSGKISLLLWRTCFASSLTPATCYL
Q*RARSASASTKPEV*SMS*RIKKFIFKGGELP
VP*PWKLEKSYCSQLT*KLKSLWVGESVNFRTAG
KTWSPSWQR*LSSSRAL*RPGTSVKRKSRS*KA
GCKF*RHKVKILFQSFTRTSITKKS*LRN*NSL
WLAGLRT*KNFKL*RRT*PGTFSWKM*WF*RSK*
SICTDNGRTSA*GWPYVNRRLKTDSIHGLYSMK
KIKSCVPGWCRWKTKFYRQRTLVLKK*LKSYRR
TAWKK*TCLVKTSYS*SRWVTS*SRPATNQEQLR
SMTSSTKLTIVGNIFLMSSDQG*RS*RRPLLLF
SSWTKT*ATFAPGWLELSLSFPSLLFMMSAMIK
RSRRGSLSSSPPMGYSRNII***LKMPCLFVKRF
SSFFL*PCIFSS*MLFFPNWGTASLSSSKDKTP
ETPPLSKPPQLWLDFISWGGKDLQRDIEQHSAG
VESVFNICDVLLHDSDACANETECDSIQQTTRSL
DRRWRNICAMSMERRMKIEETWRLWQKFLDDYS
RFEDWLKSAERTAACPNSSEVLYTSAKEELKRF
EAFQRQIHERLTQLELINKQYRRLARENRTDTAS
RLKQMVHEGNQRWDNLQRRVTAVLRRLRHFTNQ
REEFEGTRESILVWLTEMDLQLTNVEHFSESDA
DDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQK
SEPLDAVLIEDELEELHRYCQEVFGRVSRFHRR
LTSCTPGLEDEKEASENETDMEDPREIQTDSWR
KRGESEEPSSPQSLCHLVAPGHERSGCETPVSVD
SIPLEWDHTGDVGGSSSHEEDEEGPYYSALSGK
SISDGHSWHVPDSPSCPEHHYKQMEGDRNVPPV
PPASSTPYKPPYGKLLLPPGTDGGKEGPRVLNGN
PQQEDGGLAGITEQQSGAFDRWEMIQAQELHNK
LKIKQNLQQLNSDISAITTWLKKTEAELEMLKM
AKPPSDIQEIELRVKRLQEILKAFDTYKALVVSV
NVSSKEFLQTESPESTELQSRLRQLSLLWEAAQ
GAVDSWRGGLRQSLMQCQGSKTRPRSDVLFFKD
FHQLSQNLLLWLASAKNRRQKAHVTDPKADPRAL
LECRRELMQLEKELVERQPQVDMLQEISNSLLI
KGHGEDCIEAEEKVHVIEKKLKQLREQVSQDLM
ALQGTQNPASPLPSFDEVDSGDQPPATSVPAPRA
KQFRAVRTTEGEEETESRVPGSTRPQRSFLSRV
VRAALPLQLLLLLLLLLACLLPSSEEDYSCTQA
NNFARSFYPMLRYTNGPPPT*RA*LATVLHHLPD
CQGCPARGPRPI*VT*CWQGPGTCADFFWAYPA
RAPWSPGQLSDCVPPQEQGTCVAGAPGILAELV
D*FRDLWKCQFPEEPSTL*ILVCL*NSIIIM*VW
SMSSGVHHIYYRSKRGHSTLEMVQKLIGTLS*W
KQSIIFNTLRISFTPISWQF*AAGYTPKCSVQY
LALLFYIALNF*RNCIAWMWLFVHIF*QCPICMN
NVNFDFFLKKLDFSWSF*LM*SKCQTTDLIGMF
L*VNFLRLFHIQSDALLWVLTVWPRRGWGRGVG
TET*SCL*YVQLRCFSLKNKISQTSVVL
Frame 1
TESFFSTV*TKMDNAHNSTSRYSRKTSPASNGEIAVS*SNHRND*LDEQCGASNFR*RLRAFTKFC
ISS*TSSSEAQGV*NGNGL*TVDS*LR*PVITSA
KHL*CRKQAL*KNGVCRAPGGDEPPVAPCTWNA
E*KDTTFRTTSRKYH*E*K*NTDLEQLAGSTRR
ETENFTKT*KCDLSAEAAPGLSGYRKSTCN*IQS
TR*VETKLSDFGEWGSAIVRRYSIPN**VISKE
KQCSHSGQSAQNLHAVSFTGVEDL*STL**SEY
DDNPILVLHGTQQACGVIIGDLEMPGGEPSVSAR
*S*EQ*RELGETPGGYRQTQRSLPLCC*NNRRE
MPKYS*KVDSGEPSHCRPVAEGPESAPALEGL*
QCSW*SCRKAEAAGSKVSTARKHQHVWKQPGRDP
APSPAGHKGAAA*CAENKRSLSPKFQCPGSTPT
TRRVQHPHAPPGPPALSPEGCLFGKDAACESK*
I*VCSLTV*GFWSPAGIFKRSYYA*RREFG*TSP
TGKRKS*LIPESCAGTDSPIT*Y*TFE*SEPQA
PT**RSCEDVTKYEPAMDSGHGHGTGALQ*ASG
NWIE*KVSLLL*KVDPTFGEDRRSTQSGCG*QPS
*APGAAENL*DVRS*SFYKPDNC*FLCHPVLTT
PGHNRNREQTRIYYRILKADGSVAECCPGCSAE
EG*R*WAGEAVARFHYFCGELVSLPH*HQPPAIC
SEGPGALQPLPNQKSDP*AEE*RNSFSKEANYL
CPNLGSWRKVTAHN*PEN*RVCG*ENQSTSGQL
ERHGAPAGRDD*AVPEHCRDLGPV*KENQGVEKQ
AASFKGTK*RSSSRASRGPP*RKRAD*GTRTVF
G*LDSELERTSNYEGGLNPARSRGRCDGFEGAN
RAFAQTMGGPLLKGGHT*TGD*RQTQYMGCIQ*K
K*RVVCLAGADGKQSSTDSGH*Y*RND*KVTEG
LHGRNKLV**KQVTVKADG*PVDQGQQQIKSS*
DR*QAQQN*RSLATSF*CHRIKGEEAEGDLCFYS
AVGQKHEQPSHLVGSN*V*AFQACCL*CLR*SR
DPEEAR*AAAHPWGTAEILSDDD*KCHVYS*RG
FPVFSYDHAFSLHKCYFSQIGELHHYHHQRTRLQ
RPHLSPSHHSFGWTLSVGEARIYSEILNNTAQG
WSPCLTSVTSYCTTPMPVQMRPSVTRSSRPPGA
WTDAGGTFVPCPWSGA*KSRRRGACGRSF*TTIL
ALRTGSSQLRGRQPAQIPQRCCTRVPKRN*RGL
RPFSGRFMSGSLSWSSSTSSTGGWPGRTAQTRP
AG*SRWSTRATSAGTTFRGGSQPSCGDSGISPTR
GKNLRAPGRAFWCGSQRWTCS*PTWSTSQRVTP
MTRCAN*MASNRKLH*IPTRLISSLCLGSS*FR
RASPWMLC*LRMSWRNSTATARRCLEGSPGSTGG
SPPALRAWKMKRRPLRMKQTWKTPEKSRLILGV
NGERARNRHLLSPCVI*WPQGTSGLAARPLSAW
TPSPWSGTTQATWGAPPLTKRTRRAHTTAHCQVN
PFRMATRGMFPTALPVPSITTSKWKVTGMFHLF
PLRPAPLINHPMESYYYLQARMVAKKARES*MA
THSRKTGDWPVSQSSSQVPSTDGR*FKHRSFTIS
SK*NKICNS*TLISAPSLLG*KKLKQSWKC*RW
QSLPLISRK*N*E*RDCRRY*KPLTLTRH*WSL
ST*AARNFCKPRAPNPQSSKVDSAS*ACSGKQHR
AQWTAGEGAYDSRSCSARALRHGRALMCCFLRT
STS*VKICCCG*RVPRTGGRRLMSPIQRQTPGL
S*SVGGN*CNWKRSW*NVNLKWTCYRRFQTAFSL
RDMEKTVLKLKKRCMLLRRNSNSYGSKCPKI*W
PCREPRTQPHPCPASTR*TRGTSLLQHPCQLPE
QSSSEQ*ELQKARRRQRAGSPAAHGHSAPSSQGW
SGQPYPCSCSSCCCCSWPACCPPPKKTTAALRP
TTLPGPFTPC*GTPMGHPPHRGHSWPQCYTTCL
IAKGAQHVAPDQSE*LSVGKVPGPVQTSSGLTQH
GLPGAQGSFQIVFLPRSREPVWQVPRVFWQN*L
ISLGISGNVSFLKSQALCEFWFVCKTALL*CRY
GQ*AVVSITYIIEASEDIPP*KWFRNS*APLADG
NNQSYLIRLESVLLQSAGNFELPVIHQNVLFST
*LCSFILL*IFKEIVLHGCGYLCIFFNNAQSV*
IM*TSIFF*KN*ILAGAFD*CKVNAKLPT**GCF
CKLIF*DFFTSKVMLCFGF*LFGHGGGGGGGLV
QKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 2
LRASLAQFEQKWTMLITQLPDIQEKLHQLQMEKLPSRKAITEMISWMNNVEHQTSDEDSVHSPSSA
SQVKHLLQKHKEFRMEMDYKQWIVDFVNQSLLQ
LSTCDVESKRYERTEFAEHLGEMNRQWHRVHGML
NRKIQHLEQLLESITESENKIQILNNWLEAQEE
RLKTLQKPESVISVQKLLLDCQDIENQLAIKSK
ALDELKQSYLTLESGAVPLLEDTASRIDELFQKR
SSVLTQVNQLKTSMQSVLQEWKIYDQLYDEVNM
MTIRFWYCMEHSKPVVLSLETLRCQVENLQSLQ
DEAESSEGSWEKLQEVIGKLKGLCPSVAEIIEEK
CQNTHKRWTQVNQAIADQLQKAQSLLQLWKAYS
NAHGEAAARLKQQEAKFQQLANISMSGNNLAEI
LPPALQDIKELQHDVQKTKEAFLQNSSVLDRLPQ
PAESSTHMLLPGPLHSLQRAAYLEKMLLVKANE
FEFVLSQFKDFGVRLESLKGLIMHEEENLDRLH
QQEKENPDSFLNHVLALTAQSPDIEHLNEVSLKL
PLSDVAVKTLQNMNRQWIRATATALERCSELQG
IGLNEKFLYCCEKWIQLLEKIEEALKVDVANSL
PELLEQQKTYKMLEAEVSINQTIADSYVTQSLQL
LDTTEIENRPEFITEFSKLTDRWQNAVQGVRQR
KGDVDGLVRQWQDFTTSVENLFRFLTDTSHLLS
AVKGQERFSLYQTRSLIHELKNKEIHFQRRRTTC
ALTLEAGEKLLLTTDLKTKESVGRRISQLQDSW
KDMEPQLAEMIKQFQSTVETWDQCEKKIKELKS
RLQVLKAQSEDPLPELHEDLHNEKELIKELEQSL
ASWTQNLKELQTMKADLTRHVLVEDVMVLKEQI
EHLHRQWEDLCLRVAIRKQEIEDRLNTWVVFNE
KNKELCAWLVQMENKVLQTADISIEEMIEKLQKD
CMEEINLFSENKLQLKQMGDQLIKASNKSRAAE
IDDKLNKINDRWQHLFDVIGSRVKKLKETFAFI
QQLDKNMSNLRTWLARIESELSKPVVYDVCDDQE
IQKRLAEQQPTHGVQQKYYLMMTKNAMFIREEV
FQFFPMTMHFLFINVIFPKLGNCITIIIKGQDS
RDPTSLQATTALAGLYQLGRQGSTARY*TTQRRG
GVRV*HL*RPTARLRCLCK*DRV*LDPADHQEP
GQTLEEHLCHVHGAAHENRGDVAPVAEVFRRLF
SL*GLAQVS*EDGSLPKFLRGVVHECQRGTEEV*
GLSAADS*AAHSAGAHQQAVPAAGPGEPHRHGQ
QAEADGPRGQPALGQPSEAGHSRPAETQAFHQP
EGRI*GHQGEHSGVAHRDGPAADQRGALLRE*RR
*QDAPTEWLPTGNYIKYQQD*SAHCVWGAADSE
ERAPGCCAD*G*AGGTPPLLPGGVWKGLPVPPA
AHLLHSGLGR*KGGL*E*NRHGRPQRNPD*FLA*
TGRERGTVISSVPVSSSGPRARAVWLRDPCQRG
LHPPGVGPHRRRGGLLLSRRGRGGPILQRTVR*
IHFGWPLVACSRQPFLSRASLQANGR*QECSTCS
PCVQHPL*TTLWKATITSRHGWWQRRPASPEWQ
PTAGRRGTGRYHRAAVRCLRQMGDDSSTGASQ*
AQNKTKFATAEL*YQRHHYLAEKN*SRAGNVKDG
KASL*YPGNRTESEETAGDTESL*HLQGISGLC
QREQQGISANREPRIHRAPK*TPPAEPALGSST
GRSGQLERGLTTVAHAVPGL*DTAAL*CVVF*GL
PPVESKSAAVVSECQEPEAEGSCHRSKGRPPGS
PRVSEGTNATGKGAGRTSTSSGHVTGDFKQPSH
*GTWRRLY*S*RKGACY*EETQTVTGASVPRFNG
LAGNPEPSLTPAQLRRGRLGGPASCNIRASSPS
KAVQSSENYRRRGGDREQGPRQHTATALLPLKG
GPGSPTPAAAPPAAAAPGLPAALLRRRLQLHSGQ
QLCPVLLPHAEVHQWATPHIEGIAGHSATPPA*
LPRVPSTWPQTNLSDLVLARSRDLCRLLLGLPS
TGSLEPRAAFRLCSSPGAGNLCGRCPGYFGRTS*
LV*GSLEMSVS*RAKHFVNSGLFVKQHYYNVGM
VNEQWCPSHIL*KQARTFHPRNGSETHRHP*LM
ETINHI*YA*NQFYSNQLAILSCRLYTKMFCSVP
SSALLYCFKFLKKLYCMDVVICAYFLTMPNLYE
*CKLRFFFKKIRF*LELLTNVK*MPNYRLDRDV
FVS*FSKTFSHPK*CFALGFNCLATAGVGAGGWY
RNLKLFVICTTQMFLIKKQN*PDFCCTE
Sequence SYNE2.dAug05
GAAGAAGCACTCAAAGTGGATGTGGCTAACAGCCTTCCTGAGCTCCTGGAGCAGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGACAATTGCTGATTCCTATGTCACCCAGTCCTTACAACTCCTGGACACAACAGAAATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGA
TCGGTGGCAGAATGCTGTCCAGGGTGTTCGGCAGAGGAAGGGTGACGTTGATGGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTG
TTTCGCTTCCTCACTGACACCAGCCACCTGCTATCTGCAGTGAAGGGCCAGGAGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGA
ATAAAGAAATTCATTTTCAAAGGAGGCGAACTACCTGTGCCCTAACCTTGGAAGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTC
TGTGGGTAGGAGAATCAGTCAACTTCAGGACAGCTGGAAAGACATGGAGCCCCAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGG
GACCAGTGTGAAAAGAAAATCAAGGAGTTGAAAAGCAGGCTGCAAGTTTTAAAGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATA
ACGAAAAAGAGCTGATTAAGGAACTAGAACAGTCTTTGGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGT
TCTCGTGGAAGATGTGATGGTTTTGAAGGAGCAAATAGAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATT
GAAGACAGACTCAATACATGGGTTGTATTCAATGAAAAAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACA
TTAGTATTGAAGAAATGATTGAAAAGTTACAGAAGGACTGCATGGAAGAAATAAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCA
GTTGATCAAGGCCAGCAACAAATCAAGAGCAGCTGAGATCGATGACAAGCTCAACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCA
AGGGTGAAGAAGCTGAAGGAGACCTTTGCTTTTATTCAGCAGTTGGACAAAAACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTT
CCAAGCCTGTTGTTTATGATGTCTGCGATGATCAAGAGATCCAGAAGAGGCTCGCTGAGCAGCAGGATCTACAGCGAGATATTGAACAACACAGCGCAGG
GGTGGAGTCCGTGTTTAACATCTGTGACGTCCTACTGCACGACTCCGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGC
CTGGACAGACGCTGGAGGAACATTTGTGCCATGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATT
CTCGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTT
TGAGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCC
AGCAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACC
AGAGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGC
CGATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTACATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAG
AAGAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGC
GGCTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCG
TAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTG
GACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTA
AATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGT
TCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGC
AACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATA
AGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGAT
GGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCT
GTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCAC
AGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTGCCAGGGCTCTAAGACACGGCCGCGCTCTGATGTGTTGTTTTTTAAGGA
CTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCCCGGGCT
CTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCA
TTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGATTTAAT
GGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGA
GCAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCTCAAGGG
TGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGC
CAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCACCTGCCT
GATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTTACCCAG
CACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGAACTAGT
TGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATGTAGGTA
TGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTAGCTGAT
GGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGTTCAGTA
CCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATCTGTATG
AATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAGGGATGT
TTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGGGGTTGG
TACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
EEALKVDVANSLPELLEQQKTYKMLEAEVSINQTIADSYVTQSLQLLDTTEIENRPEFITEFSKLTD
RWQNAVQGVRQRKGDVDGLVRQWQDFTTSVENL
FRFLTDTSHLLSAVKGQERFSLYQTRSLIHELK
NKEIHFQRRRTTCALTLEAGEKLLLTTDLKTKES
VGRRISQLQDSWKDMEPQLAEMIKQFQSTVETW
DQCEKKIKELKSRLQVLKAQSEDPLPELHEDLH
NEKELIKELEQSLASWTQNLKELQTMKADLTRHV
LVEDVMVLKEQIEHLHRQWEDLCLRVAIRKQEI
EDRLNTWVVFNEKNKELCAWLVQMENKVLQTAD
ISIEEMIEKLQKDCMEEINLFSENKLQLKQMGDQ
LIKASNKSRAAEIDDKLNKINDRWQHLFDVIGS
RVKKLKETFAFIQQLDKNMSNLRTWLARIESEL
SKPVVYDVCDDQEIQKRLAEQQDLQRDIEQHSAG
VESVFNICDVLLHDSDACANETECDSIQQTTRS
LDRRWRNICAMSMERRMKIEETWRLWQKFLDDY
SRFEDWLKSAERTAACPNSSEVLYTSAKEELKRF
EAFQRQIHERLTQLELINKQYRRLARENRTDTA
SRLKQMVHEGNQRWDNLQRRVTAVLRRLRHFTN
QREEFEGTRESILVWLTEMDLQLTNVEHFSESDA
DDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQ
KSEPLDAVLIEDELEELHRYCQEVFGRVSRFHR
RLTSCTPGLEDEKEASENETDMEDPREIQTDSWR
KRGESEEPSSPQSLCHLVAPGHERSGCETPVSV
DSIPLEWDHTGDVGGSSSHEEDEEGPYYSALSG
KSISDGHSWHVPDSPSCPEHHYKQMEGDRNVPPV
PPASSTPYKPPYGKLLLPPGTDGGKEGPRVLNG
NPQQEDGGLAGITEQQSGAFDRWEMIQAQELHN
KLKIKQNLQQLNSDISAITTWLKKTEAELEMLKM
AKPPSDIQEIELRVKRLQEILKAFDTYKALVVS
VNVSSKEFLQTESPESTELQSRLRQLSLLWEAA
QGAVDSWRGGLRQSLMQCQGSKTRPRSDVLFFKD
FHQLSQNLLLWLASAKNRRQKAHVTDPKADPRA
LLECRRELMQLEKELVERQPQVDMLQEISNSLL
IKGHGEDCIEAEEKVHVIEKKLKQLREQVSQDLM
ALQGTQNPASPLPSFDEVDSGDQPPATSVPAPR
AKQFRAVRTTEGEEETESRVPGSTRPQRSFLSR
VVRAALPLQLLLLLLLLLACLLPSSEEDYSCTQA
NNFARSFYPMLRYTNGPPPT*RA*LATVLHHLP
DCQGCPARGPRPI*VT*CWQGPGTCADFFWAYP
ARAPWSPGQLSDCVPPQEQGTCVAGAPGILAELV
D*FRDLWKCQFPEEPSTL*ILVCL*NSIIIM*V
WSMSSGVHHIYYRSKRGHSTLEMVQKLIGTLS*
WKQSIIFNTLRISFTPISWQF*AAGYTPKCSVQY
LALLFYIALNF*RNCIAWMWLFVHIF*QCPICM
NNVNFDFFLKKLDFSWSF*LM*SKCQTTDLIGM
FL*VNFLRLFHIQSDALLWVLTVWPRRGWGRGVG
TET*SCL*YVQLRCFSLKNKISQTSVVL
Frame 1
KKHSKWMWLTAFLSSWSSRKPIRC*KLKFL*TRQLLIPMSPSPYNSWTQQK*RTDQNLLQNSQS*R
IGGRMLSRVFGRGRVTLMGW*GSGKISLLLWRTC
FASSLTPATCYLQ*RARSASASTKPEV*SMS*R
IKKFIFKGGELPVP*PWKLEKSYCSQLT*KLKS
LWVGESVNFRTAGKTWSPSWQR*LSSSRAL*RPG
TSVKRKSRS*KAGCKF*RHKVKILFQSFTRTSI
TKKS*LRN*NSLWLAGLRT*KNFKL*RRT*PGT
FSWKM*WF*RSK*SICTDNGRTSA*GWPYVNRRL
KTDSIHGLYSMKKIKSCVPGWCRWKTKFYRQRT
LVLKK*LKSYRRTAWKK*TCLVKTSYS*SRWVT
S*SRPATNQEQLRSMTSSTKLTIVGNIFLMSSDQ
G*RS*RRPLLLFSSWTKT*ATFAPGWLELSLSF
PSLLFMMSAMIKRSRRGSLSSRIYSEILNNTAQ
GWSPCLTSVTSYCTTPMPVQMRPSVTRSSRPPGA
WTDAGGTFVPCPWSGA*KSRRRGACGRSF*TTI
LALRTGSSQLRGRQPAQIPQRCCTRVPKRN*RG
LRPFSGRFMSGSLSWSSSTSSTGGWPGRTAQTRP
AG*SRWSTRATSAGTTFRGGSQPSCGDSGISPT
RGKNLRAPGRAFWCGSQRWTCS*PTWSTSQRVT
PMTRCAN*MASNRKLH*IPTRLISSLCLGSS*FR
RASPWMLC*LRMSWRNSTATARRCLEGSPGSTG
GSPPALRAWKMKRRPLRMKQTWKTPEKSRLILG
VNGERARNRHLLSPCVI*WPQGTSGLAARPLSAW
TPSPWSGTTQATWGAPPLTKRTRRAHTTAHCQV
NPFRMATRGMFPTALPVPSITTSKWKVTGMFHL
FPLRPAPLINHPMESYYYLQARMVAKKARES*MA
THSRKTGDWPVSQSSSQVPSTDGR*FKHRSFTI
SSK*NKICNS*TLISAPSLLG*KKLKQSWKC*R
WQSLPLISRK*N*E*RDCRRY*KPLTLTRH*WSL
ST*AARNFCKPRAPNPQSSKVDSAS*ACSGKQH
RAQWTAGEGAYDSRSCSARALRHGRALMCCFLR
TSTS*VKICCCG*RVPRTGGRRLMSPIQRQTPGL
S*SVGGN*CNWKRSW*NVNLKWTCYRRFQTAFS
LRDMEKTVLKLKKRCMLLRRNSNSYGSKCPKI*
WPCREPRTQPHPCPASTR*TRGTSLLQHPCQLPE
QSSSEQ*ELQKARRRQRAGSPAAHGHSAPSSQG
WSGQPYPCSCSSCCCCSWPACCPPPKKTTAALR
PTTLPGPFTPC*GTPMGHPPHRGHSWPQCYTTCL
IAKGAQHVAPDQSE*LSVGKVPGPVQTSSGLTQ
HGLPGAQGSFQIVFLPRSREPVWQVPRVFWQN*
LISLGISGNVSFLKSQALCEFWFVCKTALL*CRY
GQ*AVVSITYIIEASEDIPP*KWFRNS*APLAD
GNNQSYLIRLESVLLQSAGNFELPVIHQNVLFS
T*LCSFILL*IFKEIVLHGCGYLCIFFNNAQSV*
IM*TSIFF*KN*ILAGAFD*CKVNAKLPT**GC
FCKLIF*DFFTSKVMLCFGF*LFGHGGGGGGGL
VQKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 2
RSTQSGCG*QPS*APGAAENL*DVRS*SFYKPDNC*FLCHPVLTTPGHNRNREQTRIYYRILKADG
SVAECCPGCSAEEG*R*WAGEAVARFHYFCGEL
VSLPH*HQPPAICSEGPGALQPLPNQKSDP*AEE
*RNSFSKEANYLCPNLGSWRKVTAHN*PEN*RV
CG*ENQSTSGQLERHGAPAGRDD*AVPEHCRDL
GPV*KENQGVEKQAASFKGTK*RSSSRASRGPP*
RKRAD*GTRTVFG*LDSELERTSNYEGGLNPAR
SRGRCDGFEGANRAFAQTMGGPLLKGGHT*TGD
*RQTQYMGCIQ*KK*RVVCLAGADGKQSSTDSGH
*Y*RND*KVTEGLHGRNKLV**KQVTVKADG*P
VDQGQQQIKSS*DR*QAQQN*RSLATSF*CHRI
KGEEAEGDLCFYSAVGQKHEQPSHLVGSN*V*AF
QACCL*CLR*SRDPEEAR*AAGSTARY*TTQRR
GGVRV*HL*RPTARLRCLCK*DRV*LDPADHQE
PGQTLEEHLCHVHGAAHENRGDVAPVAEVFRRLF
SL*GLAQVS*EDGSLPKFLRGVVHECQRGTEEV
*GLSAADS*AAHSAGAHQQAVPAAGPGEPHRHG
QQAEADGPRGQPALGQPSEAGHSRPAETQAFHQP
EGRI*GHQGEHSGVAHRDGPAADQRGALLRE*R
R*QDAPTEWLPTGNYIKYQQD*SAHCVWGAADS
EERAPGCCAD*G*AGGTPPLLPGGVWKGLPVPPA
AHLLHSGLGR*KGGL*E*NRHGRPQRNPD*FLA
*TGRERGTVISSVPVSSSGPRARAVWLRDPCQR
GLHPPGVGPHRRRGGLLLSRRGRGGPILQRTVR*
IHFGWPLVACSRQPFLSRASLQANGR*QECSTC
SPCVQHPL*TTLWKATITSRHGWWQRRPASPEW
QPTAGRRGTGRYHRAAVRCLRQMGDDSSTGASQ*
AQNKTKFATAEL*YQRHHYLAEKN*SRAGNVKD
GKASL*YPGNRTESEETAGDTESL*HLQGISGL
CQREQQGISANREPRIHRAPK*TPPAEPALGSST
GRSGQLERGLTTVAHAVPGL*DTAAL*CVVF*G
LPPVESKSAAVVSECQEPEAEGSCHRSKGRPPG
SPRVSEGTNATGKGAGRTSTSSGHVTGDFKQPSH
*GTWRRLY*S*RKGACY*EETQTVTGASVPRFN
GLAGNPEPSLTPAQLRRGRLGGPASCNIRASSP
SKAVQSSENYRRRGGDREQGPRQHTATALLPLKG
GPGSPTPAAAPPAAAAPGLPAALLRRRLQLHSG
QQLCPVLLPHAEVHQWATPHIEGIAGHSATPPA
*LPRVPSTWPQTNLSDLVLARSRDLCRLLLGLPS
TGSLEPRAAFRLCSSPGAGNLCGRCPGYFGRTS
*LV*GSLEMSVS*RAKHFVNSGLFVKQHYYNVG
MVNEQWCPSHIL*KQARTFHPRNGSETHRHP*LM
ETINHI*YA*NQFYSNQLAILSCRLYTKMFCSV
PSSALLYCFKFLKKLYCMDVVICAYFLTMPNLY
E*CKLRFFFKKIRF*LELLTNVK*MPNYRLDRDV
FVS*FSKTFSHPK*CFALGFNCLATAGVGAGGW
YRNLKLFVICTTQMFLIKKQN*PDFCCTE
Sequence SYNE2.cAug05
TGCTGCTTGCAGCTTTTTAGATCAGGACTGCAATGGAGATACAATTAAATCCTAAAGGTACTTTAGAAGCAGGGGGTGTTGTAATGTCTTTTCTAGATAATGGTGTGACACTATGAAGAGATATCAAGTTTAATTCTTACAAATTAGAAAGCATCATCTGACTAAACTGAGTGTCTCTGGGGCTTAATCAGCTTGTATCA
CTAGTGGGGTTTTGTCATGCAAACTGCACTCCTTTAAATCATAAGGATGTGCTGGAAGGCTGCTTTCACTTTTTAGAATTAAACTTTTCCCCCAGTCTCG
TGAGTATTGCAACTCTCTTAATGCTTTTAGGAGCTTCAAAGCATCCTTAAGAAAGGGAAACTAACTTTTGAGAATATTATGGAAAAACTGCGAATCAAGT
ATTCCGAAATGTACACCATAGTCCCTGCAGAGATTGAATCCCAGGTGGAAGAATGCAGAAAAGCTTTAGAAGACATAGATGAGAAGATTAGCAATGAAGT
CTTAAAAAGCTCACCATCATATGCAATGAGGAGAAAAATAGAAGAAATTAACAATGGGCTTCATAATGTTGAAAAGATGTTGCAGCAGAAAAGCAAAAAT
ATTGAGAAAGCTCAAGAAATTCAAAAGAAAATGTGGGACGAGTTAGATCTATGGCATTCCAAACTAAATGAGCTGGATTCTGAAGTTCAGGACATTGTTG
AACAGGACCCAGGACAGGCTCAAGAATGGATGGATAACTTGATGATTCCTTTCCAGCAGTATCAGCAAGTATCACAGAGAGCAGAGTGTAGAACCTCACA
GTTGAATAAGGCCACAGTTAAGATGGAGGAATATAGTGACCTTCTGAAGAGCACTGAGGCTTGGATAGAAAATACCAGTCATTTGCTGGCCAATCCTGCT
GACTATGACTCTTTGAGGACACTGAGTCACCATGCTAGCACTGTGCAGGATTCCAAGTGTTTTAGAAGTGGACCAAGACCAAATATTTATGTTTCTTATT
ATGTTACAGTTATACAGATGGCTTTGGAAGATTCAGAACAGAAGCACAATCTTTTACATTCAATCTTTATGGATCTAGAAGACCTGTCAATAATTTTTGA
AACAGATGAATTAACCCAATCCATACAAGAGTTAAGTAATCAAGTAACAGCTTTACAACAAAAAATAATGGAAAGCCTTCCACAGATTCAGCGAATGGCT
GATGATGTGGTTGCTATTGAATCTGAAGTAAAATCAATGGAAAAAAGAGTTTCAAAAATCAAAACTATCCTATTATCAAAAGAAATATTTGATTTTTCAC
CTGAAGAACATCTCAAACATGGGGAGGTCATACTTGAAAATATACGTCCCATGAAGAAAACCATTGCTGAGATAGTGTCTTACCAAGTGGAACTGAGGTT
GCCCCAAACAGGAATGAAACCTCTGCCTGTGTTTCAGCGGACAAATCAGCTTTTACAAGATATAAAACTATTGGAAAATGTGACTCAAGAACAAAATGAG
TTATTAAAGGTAGTCATAAAACAGACCAATGAATGGGATGAAGAAATAGAAAATTTGAAACAGATCTTAAATAATTATTCAGCTCAGTTCTCCCTTGAAC
ATATGTCACCAGACCAAGCTGACAAGCTGCCACAACTACAGGGAGAAATCGAACGTATGGAGAAACAGATTCTGAGTTTGAACCAGAGAAAAGAAGACCT
GTTGGTGGACTTGAAGGCCACCGTACTAAACCTTCACCAGCATTTGAAGCAAGAACAAGAAGGAGTAGAAAGAGATAGGCTGCCAGCTGTAACATCAGAG
GAAGGTGGAGTGGCAGAGAGGGATGCTTCTGAGCGGAAGTTGAACAGAAGAGGCTCCATGTCTTACCTGGCAGCAGTCGAGGAAGAGGTGGAAGAAAGTT
CCGTGAAGAGCGATAATGGAGATGAGAAGGCAGAGCCATCGCCTCAGTCTTGGTCTTCACTTTGGAAGCATGACAAGGACATGGAAGAAGACAGAGCTTC
CTCATCCTCTGGAACAATTGTTCAGGAAGCATATGGGAAAATAAGCACCTCTGATAATTCCATGGCACAAATCCTCACACCAGACTCACTAAACACTGAG
CAAGGCCCAGAATGTTCCCTAAGGCCCAACCAAACAGAAGAGGGCACCACACCTCCTATTGAGGCTGACACTCTGGACTCTTCTGACGCGCAAGGAGGTT
TGGAGCCCAGGGTGGAGAAAACTAGGCCGGAGCCCACAGAAGTCCTGCATGCCTGCAAGACCCAGGTGGCCGAGCTGGAGCTGTGGCTGCAACAAGCCAA
CGTGGCAGTTGAGCCGGAAACATTAAACGCAGACATGCAGCAGGTGCTGGAACAGCAGCTGGTAGGGTGCCAGGCTATGCTAACAGAGATTGAGCACAAG
GTTGCCTTTCTGTTAGAGACTTGCAAAGATCAGGGCCTGGGAGATAATGGAGCCACTCAACATGAGGCTGAAGCGCTTTCCCTGAAACTGAAAACAGTGA
AGTGCAATTTAGAAAAAGTCCAGATGATGCTTCAGGAGAAGCACAGTGAAGATCAGCATCCTACCATTCTAAAGAAATCCTCAGAGCCAGAGCATCAAGA
AGCTCTCCAACCAGTTAACCTTTCTGAATTGGAATCCATTGTAACTGAAAGGCCACAATTCAGCAGACAAAAAGATTTCCAGCAGCAACAGGTTCTGGAG
TTAAAACCAATGGAACAGAAAGATTTCATCAAATTCATAGAATTTAATGCTAAGAAAATGTGGCCCCAGTATTGCCAACATGATAACGATACAACTCAGG
AATCATCTGCAAGCAACCAGGCATCCAGCCCTGAAAATGACGTTCCAGACTCGATCTTGTCACCCCAGGGCCAAAATGGAGATAAGTGGCAATATCTGCA
TCATGAACTCTCATCAAAAATAAAGCTCCCACTCCCTCAGCTTGTGGAGCCTCAGGTTTCCACAAATATGGGTATTCTACCCAGCGTGACTATGTATAAC
TTTAGATACCCAACAACTGAAGAACTGAAAACCTATACCACCCAACTTGAAGACCTGCGCCAAGAAGCAAGTAACCTTCAGACACAGGAAAATATGACAG
AAGAAGCATATATCAATTTGGATAAAAAATTGTTTGAACTATTCCTGACCCTCAGTCAGTGCCTCAGCAGTGTGGAGGAGATGCTGGAGATGCCCAGACT
TTACAGGGAGGATGGTTCTGGCCAGCAGGTGCACTACGAGACGCTGGCTCTTGAGTTGAAGAAACTTTATTTAGCGCTAAGTGACAAGAAGGGTGATCTT
TTGAAAGCCATGACTTGGCCTGGCGAGAACACCAACTTGCTCCTTGAATGTTTTGACAACCTTCAAGTCTGCCTGGAGCACACTCAGGCTGCAGCTGTCT
GTAGAAGCAAGTCCCTGAAAGCTGGCCTCGATTACAACCGCAGTTACCAGAATGAAATAAAGAGATTATATCATCAGCTCATTAAGAGTAAGACATCTTT
ACAACAGTCTTTGAATGAAATCAGTGGGCAGAGTGTTGCTGAACAGCTTCAGAAAGCAGATGCATATACAGTGGAGCTGGAGAACGCCGAGAGCCGAGTG
GCCAAACTAAGAGATGAAGGGGAGAGGCTTCATTTACCTTATGCTTTACTCCAGGAGGTTTACAAATTAGAGGATGTACTTGACAGTATGTGGGGAATGC
TAAGAGCCAGGTACACAGAACTCAGCAGCCCTTTCGTCACTGAGAGCCAGCAAGATGCTTTGTTGCAAGGCATGGTGGAACTGGTGAAGATTGGGAAGGA
AAAGCTTGCTCATGGCCACTTAAAACAAACCAAAAGTAAAGTGGCGTTACAGGCTCAAATAGAAAATCACAAGGTTTTTTTCCAGAAGCTTGTTGCTGAC
ATGTTGTTGATCCAAGCATACTCTGCCAAAATACTTCCTTCTTTATTGCAAAACAGAGAGACATTTTGGGCAGAACAAGTAACAGAAGTTAAAATACTAG
AAGAAAAGTCACGCCAATGTGGTATGAAGCTGCAGAGTTTGTTGCAGAAATGGGAAGAATTTGATGAAAACTATGCATCTCTTGAAAAGGACCTGGAAAT
TCTTATATCTACATTGCCCTCTGTGAGTTTGGTGGAAGAAACAGAGGAAAGATTAGTGGAAAGGATTTCATTTTACCAGCAAATAAAAAGAAACATTGGT
GGAAAACACGCCCGGCTTTACCAAACTCTGAACGAAGGCAAACAGTTGGTGGCGTCTGTGAGCTGTCCTGAATTAGAGGGCCAGATCGCAAAACTGGAAG
AGCAGTGGTTGTCCCTGAACAAGAAAATTGACCATGAGCTCCACAGGCTGCAAGCTCTTCTCAAGCATCTGCTCAGTTATAACAGAGATTCGGATCAGTT
AACCAAGTGGTTGGAATCTTCCCAGCATACTCTGAATTACTGGAAAGAACAGTCCCTCAATGTGTCTCAGGACTTGGATACAATCAGAAGCAACATCAAC
AATTTTTTTGAGTTTTCAAAAGAAGTTGATGAAAAATCCTCCTTGAAGACTGCCGTTATCAGTATCGGGAACCAGCTTCTTCACCTGAAAGAAACTGATA
CAGCTACACTGAGAGCTTCTTTAGCACAGTTTGAACAAAAATGGACAATGCTCATAACTCAACTTCCAGATATTCAAGAAAAACTTCACCAGCTTCAAAT
GGAGAAATTGCCGTCTCGTAAAGCAATCACAGAAATGATTAGCTGGATGAACAATGTGGAGCATCAAACTTCAGATGAAGACTCCGTGCATTCACCAAGT
TCTGCATCTCAAGTTAAACATCTTCTTCAGAAGCACAAGGAGTTTAGAATGGAAATGGACTATAAACAGTGGATAGTTGACTTCGTTAACCAGTCATTAC
TTCAGCTAAGCACCTGTGATGTAGAAAGCAAGCGCTATGAAAGAACGGAGTTTGCAGAGCACCTGGGGGAGATGAACCGCCAGTGGCACCGTGTACATGG
AATGCTGAATAGAAAGATACAACATTTAGAACAACTTCTAGAAAGTATCACTGAGAGTGAAAATAAAATACAGATCTTGAACAACTGGCTGGAAGCACAA
GAAGAGAGACTGAAAACTTTACAAAAACCTGAAAGTGTGATCTCAGTGCAGAAGCTGCTCCTGGACTGTCAGGATATAGAAAATCAACTTGCAATTAAAT
CCAAAGCACTAGATGAGTTGAAACAAAGTTATCTGACTTTGGAGAGTGGGGCAGTGCCATTGTTAGAAGATACAGCATCCCGAATTGATGAGTTATTTCA
AAAGAGAAGCAGTGTTCTCACTCAGGTCAATCAGCTCAAAACCTCCATGCAGTCAGTTTTACAGGAGTGGAAGATTTATGATCAACTCTATGATGAAGTG
AATATGATGACAATCCGATTCTGGTACTGCATGGAACACAGCAAGCCTGTGGTGTTATCATTGGAGACCTTGAGATGCCAGGTGGAGAACCTTCAGTCTC
TGCAAGATGAAGCTGAGAGCAGTGAAGGGAGTTGGGAGAAACTCCAGGAGGTTATCGGCAAACTCAAAGGTCTCTGCCCCTCTGTTGCTGAAATAATCGA
AGAGAAATGCCAAAATACTCATAAAAGGTGGACTCAGGTGAACCAAGCCATTGCAGACCAGTTGCAGAAGGCCCAGAGTCTGCTCCAGCTCTGGAAGGCC
TATAGCAATGCTCATGGTGAAGCTGCCGCAAGGCTGAAGCAGCAGGAAGCAAAGTTTCAACAGCTCGCAAACATCAGCATGTCTGGAAACAACCTGGCAG
AGATCCTGCCCCCAGCCCTGCAGGACATAAAGGAGCTGCAGCATGATGTGCAGAAAACAAAAGAAGCCTTTCTCCAAAATTCCAGTGTCCTGGATCGACT
CCCACAACCCGCAGAGTCCAGCACCCACATGCTCCTCCCGGGCCCCCTGCACTCTCTCCAGAGGGCTGCTTATTTGGAAAAGATGCTGCTTGTGAAAGCA
AATGAATTTGAGTTTGTTCTCTCACAGTTTAAGGATTTTGGAGTCCGGCTGGAATCTTTAAAAGGTCTTATTATGCATGAAGAAGAGAATTTGGATAGAC
TTCACCAACAGGAAAAAGAAAATCCTGACTCATTCCTGAATCATGTGCTGGCACTGACAGCCCAATCACCTGATATTGAACATTTGAATGAAGTGAGCCT
CAAGCTCCCACTTAGTGACGTAGCTGTGAAGACGTTACAAAATATGAACCGGCAATGGATTCGGGCCACGGCCACGGCACTGGAGCGCTGCAGTGAGCTT
CAGGGAATTGGATTGAATGAAAAGTTTCTTTATTGCTGTGAAAAGTGGATCCAACTTTTGGAGAAGATAGAAGAAGCACTCAAAGTGGATGTGGCTAACA
GCCTTCCTGAGCTCCTGGAGCAGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGACAATTGCTGATTCCTATGTCACCCAGTCCTT
ACAACTCCTGGACACAACAGAAATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGATCGGTGGCAGAATGCTGTCCAGGGTGTTCGG
CAGAGGAAGGGTGACGTTGATGGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTGTTTCGCTTCCTCACTGACACCAGCCACCTGC
TATCTGCAGTGAAGGGCCAGGAGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGAATAAAGAAATTCATTTTCAAAGGAGGCGAAC
TACCTGTGCCCTAACCTTGGAAGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTCTGTGGGTAGGAGAATCAGTCAACTTCAGGAC
AGCTGGAAAGACATGGAGCCCCAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGGGACCAGTGTGAAAAGAAAATCAAGGAGTTGA
AAAGCAGGCTGCAAGTTTTAAAGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATAACGAAAAAGAGCTGATTAAGGAACTAGAACA
GTCTTTGGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGTTCTCGTGGAAGATGTGATGGTTTTGAAGGAG
CAAATAGAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATTGAAGACAGACTCAATACATGGGTTGTATTCA
ATGAAAAAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACATTAGTATTGAAGAAATGATTGAAAAGTTACA
GAAGGACTGCATGGAAGAAATAAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCAGTTGATCAAGGCCAGCAACAAATCAAGAGCA
GCTGAGATCGATGACAAGCTCAACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCAAGGGTGAAGAAGCTGAAGGAGACCTTTGCTT
TTATTCAGCAGTTGGACAAAAACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGATGA
TCAAGAGATCCAGAAGAGGCTCGCTGAGCAGCAGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGACGTC
CTACTGCACGACTCCGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTGCCA
TGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGA
GAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGG
CTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCA
ACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAG
CATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTC
CAACAGGAAATTACATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTG
AGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGA
TGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCT
CCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAG
GCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGT
TCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCA
CCCTATGGAAAGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCG
GTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCT
GAACTCTGATATCAGCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATA
GAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAA
CCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTT
ACGACAGTCGCTCATGCAGTGCCAGGACTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTC
ACCGATCCAAAGGCAGACCCCCGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGT
TACAGGAGATTTCAAACAGCCTTCTCATTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTT
ACGGGAGCAAGTGTCCCAAGATTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCT
CCTGCAACATCCGTGCCAGCTCCCCGAGCAAAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGC
CACAGCGCTCCTTCCTCTCAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGA
AGAAGACTACAGCTGCACTCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTG
GCCACAGTGCTACACCACCTGCCTGATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTG
CAGACTTCTTCTGGGCTTACCCAGCACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGC
CCCGGGTATTTTGGCAGAACTAGTTGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAA
AACAGCATTATTATAATGTAGGTATGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGA
AACTCATAGGCACCCTTAGCTGATGGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTA
TACACCAAAATGTTCTGTTCAGTACCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATT
TTTTAACAATGCCCAATCTGTATGAATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCC
AAACTACCGACTTGATAGGGATGTTTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACG
GCGGGGGTGGGGGCGGGGGGTTGGTACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCT
GTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
CCLQLFRSGLQWRYN*ILKVL*KQGVL*CLF*IMV*HYEEISSLILTN*KASSD*TECLWGLISLYH
*WGFVMQTALL*IIRMCWKAAFTF*N*TFPPVS
*VLQLS*CF*ELQSILKKGKLTFENIMEKLRIK
YSEMYTIVPAEIESQVEECRKALEDIDEKISNEV
LKSSPSYAMRRKIEEINNGLHNVEKMLQQKSKN
IEKAQEIQKKMWDELDLWHSKLNELDSEVQDIV
EQDPGQAQEWMDNLMIPFQQYQQVSQRAECRTSQ
LNKATVKMEEYSDLLKSTEAWIENTSHLLANPA
DYDSLRTLSHHASTVQDSKCFRSGPRPNIYVSY
YVTVIQMALEDSEQKHNLLHSIFMDLEDLSIIFE
TDELTQSIQELSNQVTALQQKIMESLPQIQRMA
DDVVAIESEVKSMEKRVSKIKTILLSKEIFDFS
PEEHLKHGEVILENIRPMKKTIAEIVSYQVELRL
PQTGMKPLPVFQRTNQLLQDIKLLENVTQEQNE
LLKVVIKQTNEWDEEIENLKQILNNYSAQFSLE
HMSPDQADKLPQLQGEIERMEKQILSLNQRKEDL
LVDLKATVLNLHQHLKQEQEGVERDRLPAVTSE
EGGVAERDASERKLNRRGSMSYLAAVEEEVEES
SVKSDNGDEKAEPSPQSWSSLWKHDKDMEEDRAS
SSSGTIVQEAYGKISTSDNSMAQILTPDSLNTE
QGPECSLRPNQTEEGTTPPIEADTLDSSDAQGG
LEPRVEKTRPEPTEVLHACKTQVAELELWLQQAN
VAVEPETLNADMQQVLEQQLVGCQAMLTEIEHK
VAFLLETCKDQGLGDNGATQHEAEALSLKLKTV
KCNLEKVQMMLQEKHSEDQHPTILKKSSEPEHQE
ALQPVNLSELESIVTERPQFSRQKDFQQQQVLE
LKPMEQKDFIKFIEFNAKKMWPQYCQHDNDTTQ
ESSASNQASSPENDVPDSILSPQGQNGDKWQYLH
HELSSKIKLPLPQLVEPQVSTNMGILPSVTMYN
FRYPTTEELKTYTTQLEDLRQEASNLQTQENMT
EEAYINLDKKLFELFLTLSQCLSSVEEMLEMPRL
YREDGSGQQVHYETLALELKKLYLALSDKKGDL
LKAMTWPGENTNLLLECFDNLQVCLEHTQAAAV
CRSKSLKAGLDYNRSYQNEIKRLYHQLIKSKTSL
QQSLNEISGQSVAEQLQKADAYTVELENAESRV
AKLRDEGERLHLPYALLQEVYKLEDVLDSMWGM
LRARYTELSSPFVTESQQDALLQGMVELVKIGKE
KLAHGHLKQTKSKVALQAQIENHKVFFQKLVAD
MLLIQAYSAKILPSLLQNRETFWAEQVTEVKIL
EEKSRQCGMKLQSLLQKWEEFDENYASLEKDLEI
LISTLPSVSLVEETEERLVERISFYQQIKRNIG
GKHARLYQTLNEGKQLVASVSCPELEGQIAKLE
EQWLSLNKKIDHELHRLQALLKHLLSYNRDSDQL
TKWLESSQHTLNYWKEQSLNVSQDLDTIRSNIN
NFFEFSKEVDEKSSLKTAVISIGNQLLHLKETD
TATLRASLAQFEQKWTMLITQLPDIQEKLHQLQM
EKLPSRKAITEMISWMNNVEHQTSDEDSVHSPS
SASQVKHLLQKHKEFRMEMDYKQWIVDFVNQSL
LQLSTCDVESKRYERTEFAEHLGEMNRQWHRVHG
MLNRKIQHLEQLLESITESENKIQILNNWLEAQ
EERLKTLQKPESVISVQKLLLDCQDIENQLAIK
SKALDELKQSYLTLESGAVPLLEDTASRIDELFQ
KRSSVLTQVNQLKTSMQSVLQEWKIYDQLYDEV
NMMTIRFWYCMEHSKPVVLSLETLRCQVENLQS
LQDEAESSEGSWEKLQEVIGKLKGLCPSVAEIIE
EKCQNTHKRWTQVNQAIADQLQKAQSLLQLWKA
YSNAHGEAAARLKQQEAKFQQLANISMSGNNLA
EILPPALQDIKELQHDVQKTKEAFLQNSSVLDRL
PQPAESSTHMLLPGPLHSLQRAAYLEKMLLVKA
NEFEFVLSQFKDFGVRLESLKGLIMHEEENLDR
LHQQEKENPDSFLNHVLALTAQSPDIEHLNEVSL
KLPLSDVAVKTLQNMNRQWIRATATALERCSEL
QGIGLNEKFLYCCEKWIQLLEKIEEALKVDVAN
SLPELLEQQKTYKMLEAEVSINQTIADSYVTQSL
QLLDTTEIENRPEFITEFSKLTDRWQNAVQGVR
QRKGDVDGLVRQWQDFTTSVENLFRFLTDTSHL
LSAVKGQERFSLYQTRSLIHELKNKEIHFQRRRT
TCALTLEAGEKLLLTTDLKTKESVGRRISQLQD
SWKDMEPQLAEMIKQFQSTVETWDQCEKKIKEL
KSRLQVLKAQSEDPLPELHEDLHNEKELIKELEQ
SLASWTQNLKELQTMKADLTRHVLVEDVMVLKE
QIEHLHRQWEDLCLRVAIRKQEIEDRLNTWVVF
NEKNKELCAWLVQMENKVLQTADISIEEMIEKLQ
KDCMEEINLFSENKLQLKQMGDQLIKASNKSRA
AEIDDKLNKINDRWQHLFDVIGSRVKKLKETFA
FIQQLDKNMSNLRTWLARIESELSKPVVYDVCDD
QEIQKRLAEQQDLQRDIEQHSAGVESVFNICDV
LLHDSDACANETECDSIQQTTRSLDRRWRNICA
MSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAE
RTAACPNSSEVLYTSAKEELKRFEAFQRQIHER
LTQLELINKQYRRLARENRTDTASRLKQMVHEG
NQRWDNLQRRVTAVLRRLRHFTNQREEFEGTRES
ILVWLTEMDLQLTNVEHFSESDADDKMRQLNGF
QQEITLNTNKIDQLIVFGEQLIQKSEPLDAVLI
EDELEELHRYCQEVFGRVSRFHRRLTSCTPGLED
EKEASENETDMEDPREIQTDSWRKRGESEEPSS
PQSLCHLVAPGHERSGCETPVSVDSIPLEWDHT
GDVGGSSSHEEDEEGPYYSALSGKSISDGHSWHV
PDSPSCPEHHYKQMEGDRNVPPVPPASSTPYKP
PYGKLLLPPGTDGGKEGPRVLNGNPQQEDGGLA
GITEQQSGAFDRWEMIQAQELHNKLKIKQNLQQL
NSDISAITTWLKKTEAELEMLKMAKPPSDIQEI
ELRVKRLQEILKAFDTYKALVVSVNVSSKEFLQ
TESPESTELQSRLRQLSLLWEAAQGAVDSWRGGL
RQSLMQCQDFHQLSQNLLLWLASAKNRRQKAHV
TDPKADPRALLECRRELMQLEKELVERQPQVDM
LQEISNSLLIKGHGEDCIEAEEKVHVIEKKLKQL
REQVSQDLMALQGTQNPASPLPSFDEVDSGDQP
PATSVPAPRAKFRAVRTTEGEEETESRVPGSTR
PQRSFLSRVVRAALPLQLLLLLLLLLACLLPSSE
EDYSCTQANNFARSFYPMLRYTNGPPPT*RA*L
ATVLHHLPDCQGCPARGPRPI*VT*CWQGPGTC
ADFFWAYPARAPWSPGQLSDCVPPQEQGTCVAGA
PGILAELVD*FRDLWKCQFPEEPSTL*ILVCL*
NSIIIM*VWSMSSGVHHIYYRSKRGHSTLEMVQ
KLIGTLS*WKQSIIFNTLRISFTPISWQF*AAGY
TPKCSVQYLALLFYIALNF*RNCIAWMWLFVHI
F*QCPICMNNVNFDFFLKKLDFSWSF*LM*SKC
QTTDLIGMFL*VNFLRLFHIQSDALLWVLTVWPR
RGWGRGVGTET*SCL*YVQLRCFSLKNKISQTS
VVL
Frame 1
AACSFLDQDCNGDTIKS*RYFRSRGCCNVFSR*WCDTMKRYQV*FLQIRKHHLTKLSVSGA*SACI
TSGVLSCKLHSFKS*GCAGRLLSLFRIKLFPQSR
EYCNSLNAFRSFKASLRKGN*LLRILWKNCESS
IPKCTP*SLQRLNPRWKNAEKL*KT*MRRLAMK
S*KAHHHMQ*GEK*KKLTMGFIMLKRCCSRKAKI
LRKLKKFKRKCGTS*IYGIPN*MSWILKFRTLL
NRTQDRLKNGWIT**FLSSSISKYHREQSVEPH
S*IRPQLRWRNIVTF*RALRLG*KIPVICWPILL
TMTL*GH*VTMLALCRIPSVLEVDQDQIFMFLI
MLQLYRWLWKIQNRSTIFYIQSLWI*KTCQ*FL
KQMN*PNPYKS*VIK*QLYNKK*WKAFHRFSEWL
MMWLLLNLK*NQWKKEFQKSKLSYYQKKYLIFH
LKNISNMGRSYLKIYVP*RKPLLR*CLTKWN*G
CPKQE*NLCLCFSGQISFYKI*NYWKM*LKNKMS
Y*R*S*NRPMNGMKK*KI*NRS*IIIQLSSPLN
ICHQTKLTSCHNYREKSNVWRNRF*V*TREKKT
CWWT*RPPY*TFTSI*SKNKKE*KEIGCQL*HQR
KVEWQRGMLLSGS*TEEAPCLTWQQSRKRWKKV
P*RAIMEMRRQSHRLSLGLHFGSMTRTWKKTEL
PHPLEQLFRKHMGK*APLIIPWHKSSHQTH*TLS
KAQNVP*GPTKQKRAPHLLLRLTLWTLLTRKEV
WSPGWRKLGRSPQKSCMPARPRWPSWSCGCNKP
TWQLSRKH*TQTCSRCWNSSW*GARLC*QRLSTR
LPFC*RLAKIRAWEIMEPLNMRLKRFP*N*KQ*
SAI*KKSR*CFRRSTVKISILPF*RNPQSQSIK
KLSNQLTFLNWNPL*LKGHNSADKKISSSNRFWS
*NQWNRKISSNS*NLMLRKCGPSIANMITIQLR
NHLQATRHPALKMTFQTRSCHPRAKMEISGNIC
IMNSHQK*SSHSLSLWSLRFPQIWVFYPA*LCIT
LDTQQLKN*KPIPPNLKTCAKKQVTFRHRKI*Q
KKHISIWIKNCLNYS*PSVSASAVWRRCWRCPD
FTGRMVLASRCTTRRWLLS*RNFI*R*VTRRVIF
*KP*LGLARTPTCSLNVLTTFKSAWSTLRLQLS
VEASP*KLASITTAVTRMK*RDYIISSLRVRHL
YNSL*MKSVGRVLLNSFRKQMHIQWSWRTPRAEW
PN*EMKGRGFIYLMLYSRRFTN*RMYLTVCGEC
*EPGTQNSAALSSLRASKMLCCKAWWNW*RLGR
KSLLMAT*NKPKVKWRYRLK*KITRFFSRSLLLT
CC*SKHTLPKYFLLYCKTERHFGQNK*QKLKY*
KKSHANVV*SCRVCCRNGKNLMKTMHLLKRTWK
FLYLHCPL*VWWKKQRKD*WKGFHFTSK*KETLV
ENTPGFTKL*TKANSWWRL*AVLN*RARSQNWK
SSGCP*TRKLTMSSTGCKLFSSICSVITEIRIS
*PSGWNLPSIL*ITGKNSPSMCLRTWIQSEATST
IFLSFQKKLMKNPP*RLPLSVSGTSFFT*KKLI
QLH*ELL*HSLNKNGQCS*LNFQIFKKNFTSFK
WRNCRLVKQSQK*LAG*TMWSIKLQMKTPCIHQV
LHLKLNIFFRSTRSLEWKWTINSG*LTSLTSHY
FS*APVM*KASAMKERSLQSTWGR*TASGTVYM
EC*IERYNI*NNF*KVSLRVKIKYRS*TTGWKHK
KRD*KLYKNLKV*SQCRSCSWTVRI*KINLQLN
PKH*MS*NKVI*LWRVGQCHC*KIQHPELMSYF
KREAVFSLRSISSKPPCSQFYRSGRFMINSMMK*
I**QSDSGTAWNTASLWCYHWRP*DARWRTFSL
CKMKLRAVKGVGRNSRRLSANSKVSAPLLLK*S
KRNAKILIKGGLR*TKPLQTSCRRPRVCSSSGRP
IAMLMVKLPQG*SSRKQSFNSSQTSACLETTWQ
RSCPQPCRT*RSCSMMCRKQKKPFSKIPVSWID
SHNPQSPAPTCSSRAPCTLSRGLLIWKRCCL*KQ
MNLSLFSHSLRILESGWNL*KVLLCMKKRIWID
FTNRKKKILTHS*IMCWH*QPNHLILNI*MK*A
SSSHLVT*L*RRYKI*TGNGFGPRPRHWSAAVSF
RELD*MKSFFIAVKSGSNFWRR*KKHSKWMWLT
AFLSSWSSRKPIRC*KLKFL*TRQLLIPMSPSP
YNSWTQQK*RTDQNLLQNSQS*RIGGRMLSRVFG
RGRVTLMGW*GSGKISLLLWRTCFASSLTPATC
YLQ*RARSASASTKPEV*SMS*RIKKFIFKGGE
LPVP*PWKLEKSYCSQLT*KLKSLWVGESVNFRT
AGKTWSPSWQR*LSSSRAL*RPGTSVKRKSRS*
KAGCKF*RHKVKILFQSFTRTSITKKS*LRN*N
SLWLAGLRT*KNFKL*RRT*PGTFSWKM*WF*RS
K*SICTDNGRTSA*GWPYVNRRLKTDSIHGLYS
MKKIKSCVPGWCRWKTKFYRQRTLVLKK*LKSY
RRTAWKK*TCLVKTSYS*SRWVTS*SRPATNQEQ
LRSMTSSTKLTIVGNIFLMSSDQG*RS*RRPLL
LFSSWTKT*ATFAPGWLELSLSFPSLLFMMSAM
IKRSRRGSLSSRIYSEILNNTAQGWSPCLTSVTS
YCTTPMPVQMRPSVTRSSRPPGAWTDAGGTFVP
CPWSGA*KSRRRGACGRSF*TTILALRTGSSQL
RGRQPAQIPQRCCTRVPKRN*RGLRPFSGRFMSG
SLSWSSSTSSTGGWPGRTAQTRPAG*SRWSTRA
TSAGTTFRGGSQPSCGDSGISPTRGKNLRAPGR
AFWCGSQRWTCS*PTWSTSQRVTPMTRCAN*MAS
NRKLH*IPTRLISSLCLGSS*FRRASPWMLC*L
RMSWRNSTATARRCLEGSPGSTGGSPPALRAWK
MKRRPLRMKQTWKTPEKSRLILGVNGERARNRHL
LSPCVI*WPQGTSGLAARPLSAWTPSPWSGTTQ
ATWGAPPLTKRTRRAHTTAHCQVNPFRMATRGM
FPTALPVPSITTSKWKVTGMFHLFPLRPAPLINH
PMESYYYLQARMVAKKARES*MATHSRKTGDWP
VSQSSSQVPSTDGR*FKHRSFTISSK*NKICNS
*TLISAPSLLG*KKLKQSWKC*RWQSLPLISRK*
N*E*RDCRRY*KPLTLTRH*WSLST*AARNFCK
PRAPNPQSSKVDSAS*ACSGKQHRAQWTAGEGA
YDSRSCSARTSTS*VKICCCG*RVPRTGGRRLMS
PIQRQTPGLS*SVGGN*CNWKRSW*NVNLKWTC
YRRFQTAFSLRDMEKTVLKLKKRCMLLRRNSNS
YGSKCPKI*WPCREPRTQPHPCPASTR*TRGTSL
LQHPCQLPEQSSEQ*ELQKARRRQRAGSPAAHG
HSAPSSQGWSGQPYPCSCSSCCCCSWPACCPPP
KKTTAALRPTTLPGPFTPC*GTPMGHPPHRGHSW
PQCYTTCLIAKGAQHVAPDQSE*LSVGKVPGPV
QTSSGLTQHGLPGAQGSFQIVFLPRSREPVWQV
PRVFWQN*LISLGISGNVSFLKSQALCEFWFVCK
TALL*CRYGQ*AVVSITYIIEASEDIPP*KWFR
NS*APLADGNNQSYLIRLESVLLQSAGNFELPV
IHQNVLFST*LCSFILL*IFKEIVLHGCGYLCIF
FNNAQSV*IM*TSIFF*KN*ILAGAFD*CKVNA
KLPT**GCFCKLIF*DFFTSKVMLCFGF*LFGH
GGGGGGGLVQKLEAVCDMYNSDVSH*KTKLARLL
LY*
Frame 2
LLAAF*IRTAMEIQLNPKGTLEAGGVVMSFLDNGVTL*RDIKFNSYKLESII*LN*VSLGLNQLVS
LVGFCHANCTPLNHKDVLEGCFHFLELNFSPSL
VSIATLLMLLGASKHP*ERETNF*EYYGKTANQV
FRNVHHSPCRD*IPGGRMQKSFRRHR*ED*Q*S
LKKLTIICNEEKNRRN*QWAS*C*KDVAAEKQK
Y*ESSRNSKENVGRVRSMAFQTK*AGF*SSGHC*
TGPRTGSRMDG*LDDSFPAVSASITESRV*NLT
VE*GHS*DGGI**PSEEH*GLDRKYQSFAGQSC
*L*LFEDTESPC*HCAGFQVF*KWTKTKYLCFLL
CYSYTDGFGRFRTEAQSFTFNLYGSRRPVNNF*
NR*INPIHTRVK*SSNSFTTKNNGKPSTDSANG
**CGCY*I*SKINGKKSFKNQNYPIIKRNI*FFT
*RTSQTWGGHT*KYTSHEENHC*DSVLPSGTEV
APNRNETSACVSADKSAFTRYKTIGKCDSRTK*
VIKGSHKTDQ*MG*RNRKFETDLK*LFSSVLP*T
YVTRPS*QAATTTGRNRTYGETDSEFEPEKRRP
VGGLEGHRTKPSPAFEARTRRSRKR*AASCNIR
GRWSGREGCF*AEVEQKRLHVLPGSSRGRGGRKF
REER*WR*EGRAIASVLVFTLEA*QGHGRRQSF
LILWNNCSGSIWENKHL**FHGTNPHTRLTKH*
ARPRMFPKAQPNRRGHHTSY*G*HSGLF*RARRF
GAQGGEN*AGAHRSPACLQDPGGRAGAVAATSQ
RGS*AGNIKRRHAAGAGTAAGRVPGYANRD*AQ
GCLSVRDLQRSGPGR*WSHST*G*SAFPETENSE
VQFRKSPDDASGEAQ*RSASYHSKEILRARASR
SSPTS*PF*IGIHCN*KATIQQTKRFPAATGSG
VKTNGTERFHQIHRI*C*ENVAPVLPT**RYNSG
IICKQPGIQP*K*RSRLDLVTPGPKWR*VAISA
S*TLIKNKAPTPSACGASGFHKYGYSTQRDYV*
L*IPNN*RTENLYHPT*RPAPRSK*PSDTGKYDR
RSIYQFG*KIV*TIPDPQSVPQQCGGDAGDAQT
LQGGWFWPAGALRDAGS*VEETLFSAK*QEG*S
FESHDLAWREHQLAP*MF*QPSSLPGAHSGCSCL
*KQVPESWPRLQPQLPE*NKEIISSAH*E*DIF
TTVFE*NQWAECC*TASESRCIYSGAGERREPS
GQTKR*RGEASFTLCFTPGGLQIRGCT*QYVGNA
KSQVHRTQQPFRH*EPARCFVARHGGTGEDWEG
KACSWPLKTNQK*SGVTGSNRKSQGFFPEACC*
HVVDPSILCQNTSFFIAKQRDILGRTSNRS*NTR
RKVTPMWYEAAEFVAEMGRI**KLCIS*KGPGN
SYIYIALCEFGGRNRGKISGKDFILPANKKKHW
WKTRPALPNSERRQTVGGVCELS*IRGPDRKTGR
AVVVPEQEN*P*APQAASSSQASAQL*QRFGSV
NQVVGIFPAYSELLERTVPQCVSGLGYNQKQHQ
QFF*VFKRS**KILLEDCRYQYREPASSPERN*Y
SYTESFFSTV*TKMDNAHNSTSRYSRKTSPASN
GEIAVS*SNHRND*LDEQCGASNFR*RLRAFTK
FCISS*TSSSEAQGV*NGNGL*TVDS*LR*PVIT
SAKHL*CRKQAL*KNGVCRAPGGDEPPVAPCTW
NAE*KDTTFRTTSRKYH*E*K*NTDLEQLAGST
RRETENFTKT*KCDLSAEAAPGLSGYRKSTCN*I
QSTR*VETKLSDFGEWGSAIVRRYSIPN**VIS
KEKQCSHSGQSAQNLHAVSFTGVEDL*STL**S
EYDDNPILVLHGTQQACGVIIGDLEMPGGEPSVS
AR*S*EQ*RELGETPGGYRQTQRSLPLCC*NNR
REMPKYS*KVDSGEPSHCRPVAEGPESAPALEG
L*QCSW*SCRKAEAAGSKVSTARKHQHVWKQPGR
DPAPSPAGHKGAAA*CAENKRSLSPKFQCPGST
PTTRRVQHPHAPPGPPALSPEGCLFGKDAACES
K*I*VCSLTV*GFWSPAGIFKRSYYA*RREFG*T
SPTGKRKS*LIPESCAGTDSPIT*Y*TFE*SEP
QAPT**RSCEDVTKYEPAMDSGHGHGTGALQ*A
SGNWIE*KVSLLL*KVDPTFGEDRRSTQSGCG*Q
PS*APGAAENL*DVRS*SFYKPDNC*FLCHPVL
TTPGHNRNREQTRIYYRILKADGSVAECCPGCS
AEEG*R*WAGEAVARFHYFCGELVSLPH*HQPPA
ICSEGPGALQPLPNQKSDP*AEE*RNSFSKEAN
YLCPNLGSWRKVTAHN*PEN*RVCG*ENQSTSG
QLERHGAPAGRDD*AVPEHCRDLGPV*KENQGVE
KQAASFKGTK*RSSSRASRGPP*RKRAD*GTRT
VFG*LDSELERTSNYEGGLNPARSRGRCDGFEG
ANRAFAQTMGGPLLKGGHT*TGD*RQTQYMGCIQ
*KK*RVVCLAGADGKQSSTDSGH*Y*RND*KVT
EGLHGRNKLV**KQVTVKADG*PVDQGQQQIKS
S*DR*QAQQN*RSLATSF*CHRIKGEEAEGDLCF
YSAVGQKHEQPSHLVGSN*V*AFQACCL*CLR*
SRDPEEAR*AAGSTARY*TTQRRGGVRV*HL*R
PTARLRCLCK*DRV*LDPADHQEPGQTLEEHLCH
VHGAAHENRGDVAPVAEVFRRLFSL*GLAQVS*
EDGSLPKFLRGVVHECQRGTEEV*GLSAADS*A
AHSAGAHQQAVPAAGPGEPHRHGQQAEADGPRGQ
PALGQPSEAGHSRPAETQAFHQPEGRI*GHQGE
HSGVAHRDGPAADQRGALLRE*RR*QDAPTEWL
PTGNYIKYQQD*SAHCVWGAADSEERAPGCCAD*
G*AGGTPPLLPGGVWKGLPVPPAAHLLHSGLGR
*KGGL*E*NRHGRPQRNPD*FLA*TGRERGTVI
SSVPVSSSGPRARAVWLRDPCQRGLHPPGVGPHR
RRGGLLLSRRGRGGPILQRTVR*IHFGWPLVAC
SRQPFLSRASLQANGR*QECSTCSPCVQHPL*T
TLWKATITSRHGWWQRRPASPEWQPTAGRRGTGR
YHRAAVRCLRQMGDDSSTGASQ*AQNKTKFATA
EL*YQRHHYLAEKN*SRAGNVKDGKASL*YPGN
RTESEETAGDTESL*HLQGISGLCQREQQGISAN
REPRIHRAPK*TPPAEPALGSSTGRSGQLERGL
TTVAHAVPGLPPVESKSAAVVSECQEPEAEGSC
HRSKGRPPGSPRVSEGTNATGKGAGRTSTSSGHV
TGDFKQPSH*GTWRRLY*S*RKGACY*EETQTV
TGASVPRFNGLAGNPEPSLTPAQLRRGRLGGPA
SCNIRASSPSKVQSSENYRRRGGDREQGPRQHTA
TALLPLKGGPGSPTPAAAPPAAAAPGLPAALLR
RRLQLHSGQQLCPVLLPHAEVHQWATPHIEGIA
GHSATPPA*LPRVPSTWPQTNLSDLVLARSRDLC
RLLLGLPSTGSLEPRAAFRLCSSPGAGNLCGRC
PGYFGRTS*LV*GSLEMSVS*RAKHFVNSGLFV
KQHYYNVGMVNEQWCPSHIL*KQARTFHPRNGSE
THRHP*LMETINHI*YA*NQFYSNQLAILSCRL
YTKMFCSVPSSALLYCFKFLKKLYCMDVVICAY
FLTMPNLYE*CKLRFFFKKIRF*LELLTNVK*MP
NYRLDRDVFVS*FSKTFSHPK*CFALGFNCLAT
AGVGAGGWYRNLKLFVICTTQMFLIKKQN*PDF
CCTE
Sequence SYNE2.bAug05
TGCTGCTTGCAGCTTTTTAGATCAGGACTGCAATGGAGATACAATTAAATCCTAAAGGTACTTTAGAAGCAGGGGGTGTTGTAATGTCTTTTCTAGATAATGGTGTGACACTATGAAGAGATATCAAGTTTAATTCTTACAAATTAGAAAGCATCATCTGACTAAACTGAGTGTCTCTGGGGCTTAATCAGCTTGTATCA
CTAGTGGGGTTTTGTCATGCAAACTGCACTCCTTTAAATCATAAGGATGTGCTGGAAGGCTGCTTTCACTTTTTAGAATTAAACTTTTCCCCCAGTCTCG
TGAGTATTGCAACTCTCTTAATGCTTTTAGGAGCTTCAAAGCATCCTTAAGAAAGGGAAACTAACTTTTGAGAATATTATGGAAAAACTGCGAATCAAGT
ATTCCGAAATGTACACCATAGTCCCTGCAGAGATTGAATCCCAGGTGGAAGAATGCAGAAAAGCTTTAGAAGACATAGATGAGAAGATTAGCAATGAAGT
CTTAAAAAGCTCACCATCATATGCAATGAGGAGAAAAATAGAAGAAATTAACAATGGGCTTCATAATGTTGAAAAGATGTTGCAGCAGAAAAGCAAAAAT
ATTGAGAAAGCTCAAGAAATTCAAAAGAAAATGTGGGACGAGTTAGATCTATGGCATTCCAAACTAAATGAGCTGGATTCTGAAGTTCAGGACATTGTTG
AACAGGACCCAGGACAGGCTCAAGAATGGATGGATAACTTGATGATTCCTTTCCAGCAGTATCAGCAAGTATCACAGAGAGCAGAGTGTAGAACCTCACA
GTTGAATAAGGCCACAGTTAAGATGGAGGAATATAGTGACCTTCTGAAGAGCACTGAGGCTTGGATAGAAAATACCAGTCATTTGCTGGCCAATCCTGCT
GACTATGACTCTTTGAGGACACTGAGTCACCATGCTAGCACTGTGCAGGATTCCAAGTGTTTTAGAAGTGGACCAAGACCAAATATTTATGTTTCTTATT
ATGTTACAGTTATACAGATGGCTTTGGAAGATTCAGAACAGAAGCACAATCTTTTACATTCAATCTTTATGGATCTAGAAGACCTGTCAATAATTTTTGA
AACAGATGAATTAACCCAATCCATACAAGAGTTAAGTAATCAAGTAACAGCTTTACAACAAAAAATAATGGAAAGCCTTCCACAGATTCAGCGAATGGCT
GATGATGTGGTTGCTATTGAATCTGAAGTAAAATCAATGGAAAAAAGAGTTTCAAAAATCAAAACTATCCTATTATCAAAAGAAATATTTGATTTTTCAC
CTGAAGAACATCTCAAACATGGGGAGGTCATACTTGAAAATATACGTCCCATGAAGAAAACCATTGCTGAGATAGTGTCTTACCAAGTGGAACTGAGGTT
GCCCCAAACAGGAATGAAACCTCTGCCTGTGTTTCAGCGGACAAATCAGCTTTTACAAGATATAAAACTATTGGAAAATGTGACTCAAGAACAAAATGAG
TTATTAAAGGTAGTCATAAAACAGACCAATGAATGGGATGAAGAAATAGAAAATTTGAAACAGATCTTAAATAATTATTCAGCTCAGTTCTCCCTTGAAC
ATATGTCACCAGACCAAGCTGACAAGCTGCCACAACTACAGGGAGAAATCGAACGTATGGAGAAACAGATTCTGAGTTTGAACCAGAGAAAAGAAGACCT
GTTGGTGGACTTGAAGGCCACCGTACTAAACCTTCACCAGCATTTGAAGCAAGAACAAGAAGGAGTAGAAAGAGATAGGCTGCCAGCTGTAACATCAGAG
GAAGGTGGAGTGGCAGAGAGGGATGCTTCTGAGCGGAAGTTGAACAGAAGAGGCTCCATGTCTTACCTGGCAGCAGTCGAGGAAGAGGTGGAAGAAAGTT
CCGTGAAGAGCGATAATGGAGATGAGAAGGCAGAGCCATCGCCTCAGTCTTGGTCTTCACTTTGGAAGCATGACAAGGACATGGAAGAAGACAGAGCTTC
CTCATCCTCTGGAACAATTGTTCAGGAAGCATATGGGAAAATAAGCACCTCTGATAATTCCATGGCACAAATCCTCACACCAGACTCACTAAACACTGAG
CAAGGCCCAGAATGTTCCCTAAGGCCCAACCAAACAGAAGAGGGCACCACACCTCCTATTGAGGCTGACACTCTGGACTCTTCTGACGCGCAAGGAGGTT
TGGAGCCCAGGGTGGAGAAAACTAGGCCGGAGCCCACAGAAGTCCTGCATGCCTGCAAGACCCAGGTGGCCGAGCTGGAGCTGTGGCTGCAACAAGCCAA
CGTGGCAGTTGAGCCGGAAACATTAAACGCAGACATGCAGCAGGTGCTGGAACAGCAGCTGGTAGGGTGCCAGGCTATGCTAACAGAGATTGAGCACAAG
GTTGCCTTTCTGTTAGAGACTTGCAAAGATCAGGGCCTGGGAGATAATGGAGCCACTCAACATGAGGCTGAAGCGCTTTCCCTGAAACTGAAAACAGTGA
AGTGCAATTTAGAAAAAGTCCAGATGATGCTTCAGGAGAAGCACAGTGAAGATCAGCATCCTACCATTCTAAAGAAATCCTCAGAGCCAGAGCATCAAGA
AGCTCTCCAACCAGTTAACCTTTCTGAATTGGAATCCATTGTAACTGAAAGGCCACAATTCAGCAGACAAAAAGATTTCCAGCAGCAACAGGTTCTGGAG
TTAAAACCAATGGAACAGAAAGATTTCATCAAATTCATAGAATTTAATGCTAAGAAAATGTGGCCCCAGTATTGCCAACATGATAACGATACAACTCAGG
AATCATCTGCAAGCAACCAGGCATCCAGCCCTGAAAATGACGTTCCAGACTCGATCTTGTCACCCCAGGGCCAAAATGGAGATAAGTGGCAATATCTGCA
TCATGAACTCTCATCAAAAATAAAGCTCCCACTCCCTCAGCTTGTGGAGCCTCAGGTTTCCACAAATATGGGTATTCTACCCAGCGTGACTATGTATAAC
TTTAGATACCCAACAACTGAAGAACTGAAAACCTATACCACCCAACTTGAAGACCTGCGCCAAGAAGCAAGTAACCTTCAGACACAGGAAAATATGACAG
AAGAAGCATATATCAATTTGGATAAAAAATTGTTTGAACTATTCCTGACCCTCAGTCAGTGCCTCAGCAGTGTGGAGGAGATGCTGGAGATGCCCAGACT
TTACAGGGAGGATGGTTCTGGCCAGCAGGTGCACTACGAGACGCTGGCTCTTGAGTTGAAGAAACTTTATTTAGCGCTAAGTGACAAGAAGGGTGATCTT
TTGAAAGCCATGACTTGGCCTGGCGAGAACACCAACTTGCTCCTTGAATGTTTTGACAACCTTCAAGTCTGCCTGGAGCACACTCAGGCTGCAGCTGTCT
GTAGAAGCAAGTCCCTGAAAGCTGGCCTCGATTACAACCGCAGTTACCAGAATGAAATAAAGAGATTATATCATCAGCTCATTAAGAGTAAGACATCTTT
ACAACAGTCTTTGAATGAAATCAGTGGGCAGAGTGTTGCTGAACAGCTTCAGAAAGCAGATGCATATACAGTGGAGCTGGAGAACGCCGAGAGCCGAGTG
GCCAAACTAAGAGATGAAGGGGAGAGGCTTCATTTACCTTATGCTTTACTCCAGGAGGTTTACAAATTAGAGGATGTACTTGACAGTATGTGGGGAATGC
TAAGAGCCAGGTACACAGAACTCAGCAGCCCTTTCGTCACTGAGAGCCAGCAAGATGCTTTGTTGCAAGGCATGGTGGAACTGGTGAAGATTGGGAAGGA
AAAGCTTGCTCATGGCCACTTAAAACAAACCAAAAGTAAAGTGGCGTTACAGGCTCAAATAGAAAATCACAAGGTTTTTTTCCAGAAGCTTGTTGCTGAC
ATGTTGTTGATCCAAGCATACTCTGCCAAAATACTTCCTTCTTTATTGCAAAACAGAGAGACATTTTGGGCAGAACAAGTAACAGAAGTTAAAATACTAG
AAGAAAAGTCACGCCAATGTGGTATGAAGCTGCAGAGTTTGTTGCAGAAATGGGAAGAATTTGATGAAAACTATGCATCTCTTGAAAAGGACCTGGAAAT
TCTTATATCTACATTGCCCTCTGTGAGTTTGGTGGAAGAAACAGAGGAAAGATTAGTGGAAAGGATTTCATTTTACCAGCAAATAAAAAGAAACATTGGT
GGAAAACACGCCCGGCTTTACCAAACTCTGAACGAAGGCAAACAGTTGGTGGCGTCTGTGAGCTGTCCTGAATTAGAGGGCCAGATCGCAAAACTGGAAG
AGCAGTGGTTGTCCCTGAACAAGAAAATTGACCATGAGCTCCACAGGCTGCAAGCTCTTCTCAAGCATCTGCTCAGTTATAACAGAGATTCGGATCAGTT
AACCAAGTGGTTGGAATCTTCCCAGCATACTCTGAATTACTGGAAAGAACAGTCCCTCAATGTGTCTCAGGACTTGGATACAATCAGAAGCAACATCAAC
AATTTTTTTGAGTTTTCAAAAGAAGTTGATGAAAAATCCTCCTTGAAGACTGCCGTTATCAGTATCGGGAACCAGCTTCTTCACCTGAAAGAAACTGATA
CAGCTACACTGAGAGCTTCTTTAGCACAGTTTGAACAAAAATGGACAATGCTCATAACTCAACTTCCAGATATTCAAGAAAAACTTCACCAGCTTCAAAT
GGAGAAATTGCCGTCTCGTAAAGCAATCACAGAAATGATTAGCTGGATGAACAATGTGGAGCATCAAACTTCAGATGAAGACTCCGTGCATTCACCAAGT
TCTGCATCTCAAGTTAAACATCTTCTTCAGAAGCACAAGGAGTTTAGAATGGAAATGGACTATAAACAGTGGATAGTTGACTTCGTTAACCAGTCATTAC
TTCAGCTAAGCACCTGTGATGTAGAAAGCAAGCGCTATGAAAGAACGGAGTTTGCAGAGCACCTGGGGGAGATGAACCGCCAGTGGCACCGTGTACATGG
AATGCTGAATAGAAAGATACAACATTTAGAACAACTTCTAGAAAGTATCACTGAGAGTGAAAATAAAATACAGATCTTGAACAACTGGCTGGAAGCACAA
GAAGAGAGACTGAAAACTTTACAAAAACCTGAAAGTGTGATCTCAGTGCAGAAGCTGCTCCTGGACTGTCAGGATATAGAAAATCAACTTGCAATTAAAT
CCAAAGCACTAGATGAGTTGAAACAAAGTTATCTGACTTTGGAGAGTGGGGCAGTGCCATTGTTAGAAGATACAGCATCCCGAATTGATGAGTTATTTCA
AAAGAGAAGCAGTGTTCTCACTCAGGTCAATCAGCTCAAAACCTCCATGCAGTCAGTTTTACAGGAGTGGAAGATTTATGATCAACTCTATGATGAAGTG
AATATGATGACAATCCGATTCTGGTACTGCATGGAACACAGCAAGCCTGTGGTGTTATCATTGGAGACCTTGAGATGCCAGGTGGAGAACCTTCAGTCTC
TGCAAGATGAAGCTGAGAGCAGTGAAGGGAGTTGGGAGAAACTCCAGGAGGTTATCGGCAAACTCAAAGGTCTCTGCCCCTCTGTTGCTGAAATAATCGA
AGAGAAATGCCAAAATACTCATAAAAGGTGGACTCAGGTGAACCAAGCCATTGCAGACCAGTTGCAGAAGGCCCAGAGTCTGCTCCAGCTCTGGAAGGCC
TATAGCAATGCTCATGGTGAAGCTGCCGCAAGGCTGAAGCAGCAGGAAGCAAAGTTTCAACAGCTCGCAAACATCAGCATGTCTGGAAACAACCTGGCAG
AGATCCTGCCCCCAGCCCTGCAGGACATAAAGGAGCTGCAGCATGATGTGCAGAAAACAAAAGAAGCCTTTCTCCAAAATTCCAGTGTCCTGGATCGACT
CCCACAACCCGCAGAGTCCAGCACCCACATGCTCCTCCCGGGCCCCCTGCACTCTCTCCAGAGGGCTGCTTATTTGGAAAAGATGCTGCTTGTGAAAGCA
AATGAATTTGAGTTTGTTCTCTCACAGTTTAAGGATTTTGGAGTCCGGCTGGAATCTTTAAAAGGTCTTATTATGCATGAAGAAGAGAATTTGGATAGAC
TTCACCAACAGGAAAAAGAAAATCCTGACTCATTCCTGAATCATGTGCTGGCACTGACAGCCCAATCACCTGATATTGAACATTTGAATGAAGTGAGCCT
CAAGCTCCCACTTAGTGACGTAGCTGTGAAGACGTTACAAAATATGAACCGGCAATGGATTCGGGCCACGGCCACGGCACTGGAGCGCTGCAGTGAGCTT
CAGGGAATTGGATTGAATGAAAAGTTTCTTTATTGCTGTGAAAAGTGGATCCAACTTTTGGAGAAGATAGAAGAAGCACTCAAAGTGGATGTGGCTAACA
GCCTTCCTGAGCTCCTGGAGCAGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGACAATTGCTGATTCCTATGTCACCCAGTCCTT
ACAACTCCTGGACACAACAGAAATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGATCGGTGGCAGAATGCTGTCCAGGGTGTTCGG
CAGAGGAAGGGTGACGTTGATGGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTGTTTCGCTTCCTCACTGACACCAGCCACCTGC
TATCTGCAGTGAAGGGCCAGGAGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGAATAAAGAAATTCATTTTCAAAGGAGGCGAAC
TACCTGTGCCCTAACCTTGGAAGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTCTGTGGGTAGGAGAATCAGTCAACTTCAGGAC
AGCTGGAAAGACATGGAGCCCCAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGGGACCAGTGTGAAAAGAAAATCAAGGAGTTGA
AAAGCAGGCTGCAAGTTTTAAAGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATAACGAAAAAGAGCTGATTAAGGAACTAGAACA
GTCTTTGGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGTTCTCGTGGAAGATGTGATGGTTTTGAAGGAG
CAAATAGAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATTGAAGACAGACTCAATACATGGGTTGTATTCA
ATGAAAAAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACATTAGTATTGAAGAAATGATTGAAAAGTTACA
GAAGGACTGCATGGAAGAAATAAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCAGTTGATCAAGGCCAGCAACAAATCAAGAGCA
GCTGAGATCGATGACAAGCTCAACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCAAGGGTGAAGAAGCTGAAGGAGACCTTTGCTT
TTATTCAGCAGTTGGACAAAAACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGATGA
TCAAGAGATCCAGAAGAGGCTCGCTGAGCAGCAGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGACGTC
CTACTGCACGACTCCGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTGCCA
TGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGA
GAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGG
CTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCA
ACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAG
CATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTC
CAACAGGAAATTACATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTG
AGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGA
TGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCT
CCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAG
GCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGT
TCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCA
CCCTATGGAAAGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCG
GTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCT
GAACTCTGATATCAGCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATA
GAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAA
CCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTT
ACGACAGTCGCTCATGCAGTGCCAGGACTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTC
ACCGATCCAAAGGCAGACCCCCGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGT
TACAGGAGATTTCAAACAGCCTTCTCATTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTT
ACGGGAGCAAGTGTCCCAAGATTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCT
CCTGCAACATCCGTGCCAGCTCCCCGAGCAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACAC
GGCCACAGCGCTCCTTCCTCTCAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTC
CGAAGAAGACTACAGCTGCACTCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAG
CTGGCCACAGTGCTACACCACCTGCCTGATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCT
GTGCAGACTTCTTCTGGGCTTACCCAGCACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGG
TGCCCCGGGTATTTTGGCAGAACTAGTTGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTG
TAAAACAGCATTATTATAATGTAGGTATGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTC
AGAAACTCATAGGCACCCTTAGCTGATGGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGG
TTATACACCAAAATGTTCTGTTCAGTACCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCAT
ATTTTTTAACAATGCCCAATCTGTATGAATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAAT
GCCAAACTACCGACTTGATAGGGATGTTTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCC
ACGGCGGGGGTGGGGGCGGGGGGTTGGTACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACT
TCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
CCLQLFRSGLQWRYN*ILKVL*KQGVL*CLF*IMV*HYEEISSLILTN*KASSD*TECLWGLISLYH
*WGFVMQTALL*IIRMCWKAAFTF*N*TFPPVS
*VLQLS*CF*ELQSILKKGKLTFENIMEKLRIK
YSEMYTIVPAEIESQVEECRKALEDIDEKISNEV
LKSSPSYAMRRKIEEINNGLHNVEKMLQQKSKN
IEKAQEIQKKMWDELDLWHSKLNELDSEVQDIV
EQDPGQAQEWMDNLMIPFQQYQQVSQRAECRTSQ
LNKATVKMEEYSDLLKSTEAWIENTSHLLANPA
DYDSLRTLSHHASTVQDSKCFRSGPRPNIYVSY
YVTVIQMALEDSEQKHNLLHSIFMDLEDLSIIFE
TDELTQSIQELSNQVTALQQKIMESLPQIQRMA
DDVVAIESEVKSMEKRVSKIKTILLSKEIFDFS
PEEHLKHGEVILENIRPMKKTIAEIVSYQVELRL
PQTGMKPLPVFQRTNQLLQDIKLLENVTQEQNE
LLKVVIKQTNEWDEEIENLKQILNNYSAQFSLE
HMSPDQADKLPQLQGEIERMEKQILSLNQRKEDL
LVDLKATVLNLHQHLKQEQEGVERDRLPAVTSE
EGGVAERDASERKLNRRGSMSYLAAVEEEVEES
SVKSDNGDEKAEPSPQSWSSLWKHDKDMEEDRAS
SSSGTIVQEAYGKISTSDNSMAQILTPDSLNTE
QGPECSLRPNQTEEGTTPPIEADTLDSSDAQGG
LEPRVEKTRPEPTEVLHACKTQVAELELWLQQAN
VAVEPETLNADMQQVLEQQLVGCQAMLTEIEHK
VAFLLETCKDQGLGDNGATQHEAEALSLKLKTV
KCNLEKVQMMLQEKHSEDQHPTILKKSSEPEHQE
ALQPVNLSELESIVTERPQFSRQKDFQQQQVLE
LKPMEQKDFIKFIEFNAKKMWPQYCQHDNDTTQ
ESSASNQASSPENDVPDSILSPQGQNGDKWQYLH
HELSSKIKLPLPQLVEPQVSTNMGILPSVTMYN
FRYPTTEELKTYTTQLEDLRQEASNLQTQENMT
EEAYINLDKKLFELFLTLSQCLSSVEEMLEMPRL
YREDGSGQQVHYETLALELKKLYLALSDKKGDL
LKAMTWPGENTNLLLECFDNLQVCLEHTQAAAV
CRSKSLKAGLDYNRSYQNEIKRLYHQLIKSKTSL
QQSLNEISGQSVAEQLQKADAYTVELENAESRV
AKLRDEGERLHLPYALLQEVYKLEDVLDSMWGM
LRARYTELSSPFVTESQQDALLQGMVELVKIGKE
KLAHGHLKQTKSKVALQAQIENHKVFFQKLVAD
MLLIQAYSAKILPSLLQNRETFWAEQVTEVKIL
EEKSRQCGMKLQSLLQKWEEFDENYASLEKDLEI
LISTLPSVSLVEETEERLVERISFYQQIKRNIG
GKHARLYQTLNEGKQLVASVSCPELEGQIAKLE
EQWLSLNKKIDHELHRLQALLKHLLSYNRDSDQL
TKWLESSQHTLNYWKEQSLNVSQDLDTIRSNIN
NFFEFSKEVDEKSSLKTAVISIGNQLLHLKETD
TATLRASLAQFEQKWTMLITQLPDIQEKLHQLQM
EKLPSRKAITEMISWMNNVEHQTSDEDSVHSPS
SASQVKHLLQKHKEFRMEMDYKQWIVDFVNQSL
LQLSTCDVESKRYERTEFAEHLGEMNRQWHRVHG
MLNRKIQHLEQLLESITESENKIQILNNWLEAQ
EERLKTLQKPESVISVQKLLLDCQDIENQLAIK
SKALDELKQSYLTLESGAVPLLEDTASRIDELFQ
KRSSVLTQVNQLKTSMQSVLQEWKIYDQLYDEV
NMMTIRFWYCMEHSKPVVLSLETLRCQVENLQS
LQDEAESSEGSWEKLQEVIGKLKGLCPSVAEIIE
EKCQNTHKRWTQVNQAIADQLQKAQSLLQLWKA
YSNAHGEAAARLKQQEAKFQQLANISMSGNNLA
EILPPALQDIKELQHDVQKTKEAFLQNSSVLDRL
PQPAESSTHMLLPGPLHSLQRAAYLEKMLLVKA
NEFEFVLSQFKDFGVRLESLKGLIMHEEENLDR
LHQQEKENPDSFLNHVLALTAQSPDIEHLNEVSL
KLPLSDVAVKTLQNMNRQWIRATATALERCSEL
QGIGLNEKFLYCCEKWIQLLEKIEEALKVDVAN
SLPELLEQQKTYKMLEAEVSINQTIADSYVTQSL
QLLDTTEIENRPEFITEFSKLTDRWQNAVQGVR
QRKGDVDGLVRQWQDFTTSVENLFRFLTDTSHL
LSAVKGQERFSLYQTRSLIHELKNKEIHFQRRRT
TCALTLEAGEKLLLTTDLKTKESVGRRISQLQD
SWKDMEPQLAEMIKQFQSTVETWDQCEKKIKEL
KSRLQVLKAQSEDPLPELHEDLHNEKELIKELEQ
SLASWTQNLKELQTMKADLTRHVLVEDVMVLKE
QIEHLHRQWEDLCLRVAIRKQEIEDRLNTWVVF
NEKNKELCAWLVQMENKVLQTADISIEEMIEKLQ
KDCMEEINLFSENKLQLKQMGDQLIKASNKSRA
AEIDDKLNKINDRWQHLFDVIGSRVKKLKETFA
FIQQLDKNMSNLRTWLARIESELSKPVVYDVCDD
QEIQKRLAEQQDLQRDIEQHSAGVESVFNICDV
LLHDSDACANETECDSIQQTTRSLDRRWRNICA
MSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAE
RTAACPNSSEVLYTSAKEELKRFEAFQRQIHER
LTQLELINKQYRRLARENRTDTASRLKQMVHEG
NQRWDNLQRRVTAVLRRLRHFTNQREEFEGTRES
ILVWLTEMDLQLTNVEHFSESDADDKMRQLNGF
QQEITLNTNKIDQLIVFGEQLIQKSEPLDAVLI
EDELEELHRYCQEVFGRVSRFHRRLTSCTPGLED
EKEASENETDMEDPREIQTDSWRKRGESEEPSS
PQSLCHLVAPGHERSGCETPVSVDSIPLEWDHT
GDVGGSSSHEEDEEGPYYSALSGKSISDGHSWHV
PDSPSCPEHHYKQMEGDRNVPPVPPASSTPYKP
PYGKLLLPPGTDGGKEGPRVLNGNPQQEDGGLA
GITEQQSGAFDRWEMIQAQELHNKLKIKQNLQQL
NSDISAITTWLKKTEAELEMLKMAKPPSDIQEI
ELRVKRLQEILKAFDTYKALVVSVNVSSKEFLQ
TESPESTELQSRLRQLSLLWEAAQGAVDSWRGGL
RQSLMQCQDFHQLSQNLLLWLASAKNRRQKAHV
TDPKADPRALLECRRELMQLEKELVERQPQVDM
LQEISNSLLIKGHGEDCIEAEEKVHVIEKKLKQL
REQVSQDLMALQGTQNPASPLPSFDEVDSGDQP
PATSVPAPRAKQFRAVRTTEGEEETESRVPGST
RPQRSFLSRVVRAALPLQLLLLLLLLLACLLPSS
EEDYSCTQANNFARSFYPMLRYTNGPPPT*RA*
LATVLHHLPDCQGCPARGPRPI*VT*CWQGPGT
CADFFWAYPARAPWSPGQLSDCVPPQEQGTCVAG
APGILAELVD*FRDLWKCQFPEEPSTL*ILVCL
*NSIIIM*VWSMSSGVHHIYYRSKRGHSTLEMV
QKLIGTLS*WKQSIIFNTLRISFTPISWQF*AAG
YTPKCSVQYLALLFYIALNF*RNCIAWMWLFVH
IF*QCPICMNNVNFDFFLKKLDFSWSF*LM*SK
CQTTDLIGMFL*VNFLRLFHIQSDALLWVLTVWP
RRGWGRGVGTET*SCL*YVQLRCFSLKNKISQT
SVVL
Frame 1
AACSFLDQDCNGDTIKS*RYFRSRGCCNVFSR*WCDTMKRYQV*FLQIRKHHLTKLSVSGA*SACI
TSGVLSCKLHSFKS*GCAGRLLSLFRIKLFPQSR
EYCNSLNAFRSFKASLRKGN*LLRILWKNCESS
IPKCTP*SLQRLNPRWKNAEKL*KT*MRRLAMK
S*KAHHHMQ*GEK*KKLTMGFIMLKRCCSRKAKI
LRKLKKFKRKCGTS*IYGIPN*MSWILKFRTLL
NRTQDRLKNGWIT**FLSSSISKYHREQSVEPH
S*IRPQLRWRNIVTF*RALRLG*KIPVICWPILL
TMTL*GH*VTMLALCRIPSVLEVDQDQIFMFLI
MLQLYRWLWKIQNRSTIFYIQSLWI*KTCQ*FL
KQMN*PNPYKS*VIK*QLYNKK*WKAFHRFSEWL
MMWLLLNLK*NQWKKEFQKSKLSYYQKKYLIFH
LKNISNMGRSYLKIYVP*RKPLLR*CLTKWN*G
CPKQE*NLCLCFSGQISFYKI*NYWKM*LKNKMS
Y*R*S*NRPMNGMKK*KI*NRS*IIIQLSSPLN
ICHQTKLTSCHNYREKSNVWRNRF*V*TREKKT
CWWT*RPPY*TFTSI*SKNKKE*KEIGCQL*HQR
KVEWQRGMLLSGS*TEEAPCLTWQQSRKRWKKV
P*RAIMEMRRQSHRLSLGLHFGSMTRTWKKTEL
PHPLEQLFRKHMGK*APLIIPWHKSSHQTH*TLS
KAQNVP*GPTKQKRAPHLLLRLTLWTLLTRKEV
WSPGWRKLGRSPQKSCMPARPRWPSWSCGCNKP
TWQLSRKH*TQTCSRCWNSSW*GARLC*QRLSTR
LPFC*RLAKIRAWEIMEPLNMRLKRFP*N*KQ*
SAI*KKSR*CFRRSTVKISILPF*RNPQSQSIK
KLSNQLTFLNWNPL*LKGHNSADKKISSSNRFWS
*NQWNRKISSNS*NLMLRKCGPSIANMITIQLR
NHLQATRHPALKMTFQTRSCHPRAKMEISGNIC
IMNSHQK*SSHSLSLWSLRFPQIWVFYPA*LCIT
LDTQQLKN*KPIPPNLKTCAKKQVTFRHRKI*Q
KKHISIWIKNCLNYS*PSVSASAVWRRCWRCPD
FTGRMVLASRCTTRRWLLS*RNFI*R*VTRRVIF
*KP*LGLARTPTCSLNVLTTFKSAWSTLRLQLS
VEASP*KLASITTAVTRMK*RDYIISSLRVRHL
YNSL*MKSVGRVLLNSFRKQMHIQWSWRTPRAEW
PN*EMKGRGFIYLMLYSRRFTN*RMYLTVCGEC
*EPGTQNSAALSSLRASKMLCCKAWWNW*RLGR
KSLLMAT*NKPKVKWRYRLK*KITRFFSRSLLLT
CC*SKHTLPKYFLLYCKTERHFGQNK*QKLKY*
KKSHANVV*SCRVCCRNGKNLMKTMHLLKRTWK
FLYLHCPL*VWWKKQRKD*WKGFHFTSK*KETLV
ENTPGFTKL*TKANSWWRL*AVLN*RARSQNWK
SSGCP*TRKLTMSSTGCKLFSSICSVITEIRIS
*PSGWNLPSIL*ITGKNSPSMCLRTWIQSEATST
IFLSFQKKLMKNPP*RLPLSVSGTSFFT*KKLI
QLH*ELL*HSLNKNGQCS*LNFQIFKKNFTSFK
WRNCRLVKQSQK*LAG*TMWSIKLQMKTPCIHQV
LHLKLNIFFRSTRSLEWKWTINSG*LTSLTSHY
FS*APVM*KASAMKERSLQSTWGR*TASGTVYM
EC*IERYNI*NNF*KVSLRVKIKYRS*TTGWKHK
KRD*KLYKNLKV*SQCRSCSWTVRI*KINLQLN
PKH*MS*NKVI*LWRVGQCHC*KIQHPELMSYF
KREAVFSLRSISSKPPCSQFYRSGRFMINSMMK*
I**QSDSGTAWNTASLWCYHWRP*DARWRTFSL
CKMKLRAVKGVGRNSRRLSANSKVSAPLLLK*S
KRNAKILIKGGLR*TKPLQTSCRRPRVCSSSGRP
IAMLMVKLPQG*SSRKQSFNSSQTSACLETTWQ
RSCPQPCRT*RSCSMMCRKQKKPFSKIPVSWID
SHNPQSPAPTCSSRAPCTLSRGLLIWKRCCL*KQ
MNLSLFSHSLRILESGWNL*KVLLCMKKRIWID
FTNRKKKILTHS*IMCWH*QPNHLILNI*MK*A
SSSHLVT*L*RRYKI*TGNGFGPRPRHWSAAVSF
RELD*MKSFFIAVKSGSNFWRR*KKHSKWMWLT
AFLSSWSSRKPIRC*KLKFL*TRQLLIPMSPSP
YNSWTQQK*RTDQNLLQNSQS*RIGGRMLSRVFG
RGRVTLMGW*GSGKISLLLWRTCFASSLTPATC
YLQ*RARSASASTKPEV*SMS*RIKKFIFKGGE
LPVP*PWKLEKSYCSQLT*KLKSLWVGESVNFRT
AGKTWSPSWQR*LSSSRAL*RPGTSVKRKSRS*
KAGCKF*RHKVKILFQSFTRTSITKKS*LRN*N
SLWLAGLRT*KNFKL*RRT*PGTFSWKM*WF*RS
K*SICTDNGRTSA*GWPYVNRRLKTDSIHGLYS
MKKIKSCVPGWCRWKTKFYRQRTLVLKK*LKSY
RRTAWKK*TCLVKTSYS*SRWVTS*SRPATNQEQ
LRSMTSSTKLTIVGNIFLMSSDQG*RS*RRPLL
LFSSWTKT*ATFAPGWLELSLSFPSLLFMMSAM
IKRSRRGSLSSRIYSEILNNTAQGWSPCLTSVTS
YCTTPMPVQMRPSVTRSSRPPGAWTDAGGTFVP
CPWSGA*KSRRRGACGRSF*TTILALRTGSSQL
RGRQPAQIPQRCCTRVPKRN*RGLRPFSGRFMSG
SLSWSSSTSSTGGWPGRTAQTRPAG*SRWSTRA
TSAGTTFRGGSQPSCGDSGISPTRGKNLRAPGR
AFWCGSQRWTCS*PTWSTSQRVTPMTRCAN*MAS
NRKLH*IPTRLISSLCLGSS*FRRASPWMLC*L
RMSWRNSTATARRCLEGSPGSTGGSPPALRAWK
MKRRPLRMKQTWKTPEKSRLILGVNGERARNRHL
LSPCVI*WPQGTSGLAARPLSAWTPSPWSGTTQ
ATWGAPPLTKRTRRAHTTAHCQVNPFRMATRGM
FPTALPVPSITTSKWKVTGMFHLFPLRPAPLINH
PMESYYYLQARMVAKKARES*MATHSRKTGDWP
VSQSSSQVPSTDGR*FKHRSFTISSK*NKICNS
*TLISAPSLLG*KKLKQSWKC*RWQSLPLISRK*
N*E*RDCRRY*KPLTLTRH*WSLST*AARNFCK
PRAPNPQSSKVDSAS*ACSGKQHRAQWTAGEGA
YDSRSCSARTSTS*VKICCCG*RVPRTGGRRLMS
PIQRQTPGLS*SVGGN*CNWKRSW*NVNLKWTC
YRRFQTAFSLRDMEKTVLKLKKRCMLLRRNSNS
YGSKCPKI*WPCREPRTQPHPCPASTR*TRGTSL
LQHPCQLPEQSSSEQ*ELQKARRRQRAGSPAAH
GHSAPSSQGWSGQPYPCSCSSCCCCSWPACCPP
PKKTTAALRPTTLPGPFTPC*GTPMGHPPHRGHS
WPQCYTTCLIAKGAQHVAPDQSE*LSVGKVPGP
VQTSSGLTQHGLPGAQGSFQIVFLPRSREPVWQ
VPRVFWQN*LISLGISGNVSFLKSQALCEFWFVC
KTALL*CRYGQ*AVVSITYIIEASEDIPP*KWF
RNS*APLADGNNQSYLIRLESVLLQSAGNFELP
VIHQNVLFST*LCSFILL*IFKEIVLHGCGYLCI
FFNNAQSV*IM*TSIFF*KN*ILAGAFD*CKVN
AKLPT**GCFCKLIF*DFFTSKVMLCFGF*LFG
HGGGGGGGLVQKLEAVCDMYNSDVSH*KTKLARL
LLY*
Frame 2
LLAAF*IRTAMEIQLNPKGTLEAGGVVMSFLDNGVTL*RDIKFNSYKLESII*LN*VSLGLNQLVS
LVGFCHANCTPLNHKDVLEGCFHFLELNFSPSL
VSIATLLMLLGASKHP*ERETNF*EYYGKTANQV
FRNVHHSPCRD*IPGGRMQKSFRRHR*ED*Q*S
LKKLTIICNEEKNRRN*QWAS*C*KDVAAEKQK
Y*ESSRNSKENVGRVRSMAFQTK*AGF*SSGHC*
TGPRTGSRMDG*LDDSFPAVSASITESRV*NLT
VE*GHS*DGGI**PSEEH*GLDRKYQSFAGQSC
*L*LFEDTESPC*HCAGFQVF*KWTKTKYLCFLL
CYSYTDGFGRFRTEAQSFTFNLYGSRRPVNNF*
NR*INPIHTRVK*SSNSFTTKNNGKPSTDSANG
**CGCY*I*SKINGKKSFKNQNYPIIKRNI*FFT
*RTSQTWGGHT*KYTSHEENHC*DSVLPSGTEV
APNRNETSACVSADKSAFTRYKTIGKCDSRTK*
VIKGSHKTDQ*MG*RNRKFETDLK*LFSSVLP*T
YVTRPS*QAATTTGRNRTYGETDSEFEPEKRRP
VGGLEGHRTKPSPAFEARTRRSRKR*AASCNIR
GRWSGREGCF*AEVEQKRLHVLPGSSRGRGGRKF
REER*WR*EGRAIASVLVFTLEA*QGHGRRQSF
LILWNNCSGSIWENKHL**FHGTNPHTRLTKH*
ARPRMFPKAQPNRRGHHTSY*G*HSGLF*RARRF
GAQGGEN*AGAHRSPACLQDPGGRAGAVAATSQ
RGS*AGNIKRRHAAGAGTAAGRVPGYANRD*AQ
GCLSVRDLQRSGPGR*WSHST*G*SAFPETENSE
VQFRKSPDDASGEAQ*RSASYHSKEILRARASR
SSPTS*PF*IGIHCN*KATIQQTKRFPAATGSG
VKTNGTERFHQIHRI*C*ENVAPVLPT**RYNSG
IICKQPGIQP*K*RSRLDLVTPGPKWR*VAISA
S*TLIKNKAPTPSACGASGFHKYGYSTQRDYV*
L*IPNN*RTENLYHPT*RPAPRSK*PSDTGKYDR
RSIYQFG*KIV*TIPDPQSVPQQCGGDAGDAQT
LQGGWFWPAGALRDAGS*VEETLFSAK*QEG*S
FESHDLAWREHQLAP*MF*QPSSLPGAHSGCSCL
*KQVPESWPRLQPQLPE*NKEIISSAH*E*DIF
TTVFE*NQWAECC*TASESRCIYSGAGERREPS
GQTKR*RGEASFTLCFTPGGLQIRGCT*QYVGNA
KSQVHRTQQPFRH*EPARCFVARHGGTGEDWEG
KACSWPLKTNQK*SGVTGSNRKSQGFFPEACC*
HVVDPSILCQNTSFFIAKQRDILGRTSNRS*NTR
RKVTPMWYEAAEFVAEMGRI**KLCIS*KGPGN
SYIYIALCEFGGRNRGKISGKDFILPANKKKHW
WKTRPALPNSERRQTVGGVCELS*IRGPDRKTGR
AVVVPEQEN*P*APQAASSSQASAQL*QRFGSV
NQVVGIFPAYSELLERTVPQCVSGLGYNQKQHQ
QFF*VFKRS**KILLEDCRYQYREPASSPERN*Y
SYTESFFSTV*TKMDNAHNSTSRYSRKTSPASN
GEIAVS*SNHRND*LDEQCGASNFR*RLRAFTK
FCISS*TSSSEAQGV*NGNGL*TVDS*LR*PVIT
SAKHL*CRKQAL*KNGVCRAPGGDEPPVAPCTW
NAE*KDTTFRTTSRKYH*E*K*NTDLEQLAGST
RRETENFTKT*KCDLSAEAAPGLSGYRKSTCN*I
QSTR*VETKLSDFGEWGSAIVRRYSIPN**VIS
KEKQCSHSGQSAQNLHAVSFTGVEDL*STL**S
EYDDNPILVLHGTQQACGVIIGDLEMPGGEPSVS
AR*S*EQ*RELGETPGGYRQTQRSLPLCC*NNR
REMPKYS*KVDSGEPSHCRPVAEGPESAPALEG
L*QCSW*SCRKAEAAGSKVSTARKHQHVWKQPGR
DPAPSPAGHKGAAA*CAENKRSLSPKFQCPGST
PTTRRVQHPHAPPGPPALSPEGCLFGKDAACES
K*I*VCSLTV*GFWSPAGIFKRSYYA*RREFG*T
SPTGKRKS*LIPESCAGTDSPIT*Y*TFE*SEP
QAPT**RSCEDVTKYEPAMDSGHGHGTGALQ*A
SGNWIE*KVSLLL*KVDPTFGEDRRSTQSGCG*Q
PS*APGAAENL*DVRS*SFYKPDNC*FLCHPVL
TTPGHNRNREQTRIYYRILKADGSVAECCPGCS
AEEG*R*WAGEAVARFHYFCGELVSLPH*HQPPA
ICSEGPGALQPLPNQKSDP*AEE*RNSFSKEAN
YLCPNLGSWRKVTAHN*PEN*RVCG*ENQSTSG
QLERHGAPAGRDD*AVPEHCRDLGPV*KENQGVE
KQAASFKGTK*RSSSRASRGPP*RKRAD*GTRT
VFG*LDSELERTSNYEGGLNPARSRGRCDGFEG
ANRAFAQTMGGPLLKGGHT*TGD*RQTQYMGCIQ
*KK*RVVCLAGADGKQSSTDSGH*Y*RND*KVT
EGLHGRNKLV**KQVTVKADG*PVDQGQQQIKS
S*DR*QAQQN*RSLATSF*CHRIKGEEAEGDLCF
YSAVGQKHEQPSHLVGSN*V*AFQACCL*CLR*
SRDPEEAR*AAGSTARY*TTQRRGGVRV*HL*R
PTARLRCLCK*DRV*LDPADHQEPGQTLEEHLCH
VHGAAHENRGDVAPVAEVFRRLFSL*GLAQVS*
EDGSLPKFLRGVVHECQRGTEEV*GLSAADS*A
AHSAGAHQQAVPAAGPGEPHRHGQQAEADGPRGQ
PALGQPSEAGHSRPAETQAFHQPEGRI*GHQGE
HSGVAHRDGPAADQRGALLRE*RR*QDAPTEWL
PTGNYIKYQQD*SAHCVWGAADSEERAPGCCAD*
G*AGGTPPLLPGGVWKGLPVPPAAHLLHSGLGR
*KGGL*E*NRHGRPQRNPD*FLA*TGRERGTVI
SSVPVSSSGPRARAVWLRDPCQRGLHPPGVGPHR
RRGGLLLSRRGRGGPILQRTVR*IHFGWPLVAC
SRQPFLSRASLQANGR*QECSTCSPCVQHPL*T
TLWKATITSRHGWWQRRPASPEWQPTAGRRGTGR
YHRAAVRCLRQMGDDSSTGASQ*AQNKTKFATA
EL*YQRHHYLAEKN*SRAGNVKDGKASL*YPGN
RTESEETAGDTESL*HLQGISGLCQREQQGISAN
REPRIHRAPK*TPPAEPALGSSTGRSGQLERGL
TTVAHAVPGLPPVESKSAAVVSECQEPEAEGSC
HRSKGRPPGSPRVSEGTNATGKGAGRTSTSSGHV
TGDFKQPSH*GTWRRLY*S*RKGACY*EETQTV
TGASVPRFNGLAGNPEPSLTPAQLRRGRLGGPA
SCNIRASSPSKAVQSSENYRRRGGDREQGPRQHT
ATALLPLKGGPGSPTPAAAPPAAAAPGLPAALL
RRRLQLHSGQQLCPVLLPHAEVHQWATPHIEGI
AGHSATPPA*LPRVPSTWPQTNLSDLVLARSRDL
CRLLLGLPSTGSLEPRAAFRLCSSPGAGNLCGR
CPGYFGRTS*LV*GSLEMSVS*RAKHFVNSGLF
VKQHYYNVGMVNEQWCPSHIL*KQARTFHPRNGS
ETHRHP*LMETINHI*YA*NQFYSNQLAILSCR
LYTKMFCSVPSSALLYCFKFLKKLYCMDVVICA
YFLTMPNLYE*CKLRFFFKKIRF*LELLTNVK*M
PNYRLDRDVFVS*FSKTFSHPK*CFALGFNCLA
TAGVGAGGWYRNLKLFVICTTQMFLIKKQN*PD
FCCTE
Sequence SYNE2.aAug05
GCCAAGGAGCGGAGCGCGCTCAGCTGCGCCCAGAGCCTTCGGCCGGACCTGAAAAAGCGAGAGGGAGAGCGAGCAAAAGGCGCGATCCAGAGAGCCAGGCAAGCGGGGCGCCGACCTCGGGCGGCCCCGGCCCGCGCCCTTGCCCCTCGGCCGGGCGGACGTTCACTTCTTCAACTGAGATGGACATGATATAATCTCCA
TTGAGTCAAAGAATGGCATCTAGTCCTGAGCTTCCCACCGAAGATGAACAGGGTTCCTGGGGCATCGACGATCTCCATATTTCATTGCAAGCTGAACAGG
AAGACACCCAGAAGAAAGCCTTCACGTGCTGGATAAACTCACAGTTGGCCAGGCACACTTCTCCCTCAGTTATATCCGACCTATTCACAGACATTAAAAA
GGGGCATGTCCTCCTGGATCTGCTAGAAGTACTTTCTGGGCAACAGTTGCCTCGGGATAAAGGATCTAATACCTTCCAGTGTAGAATCAATATAGAACAT
GCCTTGACATTCCTAAGAAACCGATCAATTAAGCTAATAAATATTCATGTTACTGATATCATTGATGGAAACCCATCCATTATCCTTGGCCTAATTTGGA
CAATTATCCTGCACTTTCATATTGAGAAGCTTGCCCAGACTCTTTCTTGCAATTACAATCAGCCTTCCCTGGATGATGTGAGTGTGGTTGACTCATCTCC
TGCCTCAAGTCCTCCAGCTAAGAAATGCTCTAAAGTGCAAGCAAGATGGCAAATGTCTGCAAGAAAGGCCCTTCTTTTGTGGGCTCAGGAACAATGCGCC
ACCTATGAGTCTGTCAATGTGACCGATTTTAAGTCAAGTTGGAGAAATGGGATGGCTTTTTTGGCCATCATTCATGCCTTGCGACCAGACCTAATTGACA
TGAAGAGTGTGAAGCATAGATCCAACAAAGACAATCTGAGAGAGGCCTTCAGAATTGCAGAACAAGAATTAAAAATCCCCAGATTGCTGGAACCAGAAGA
TGTGGATGTTGTTGATCCTGATGAAAAGTCCATCATGACCTATGTGGCACAGTTTCTGCAGTATTCCAAAGATGCCCCTGGGACTGGAGAGGAGGCTCAG
GGAAAGGTGAAAGATGCTATGGGCTGGTTAACTCTGCAAAAGGAAAAACTACAGAAGTTGCTAAAGGATTCAGAGAATGATACCTACTTTAAAAAGTATA
ATAGCCTGCTGTCCTTTATGGAGTCATTCAATGAAGAAAAAAAGTCCTTTTTGGATGTCCTGTCAATAAAACGGGATCTGGATGAGCTGGACAAGGATCA
TTTACAGTTGAGAGAAGCCTGGGATGGCCTCGATCACCAGATTAATGCATGGAAAATAAAGCTAAATTATGCCTTGCCCCCACCCCTCCATCAAACTGAA
GCTTGGCTCCAGGAGGTAGAAGAGCTTATGGATGAAGATTTGTCAGCCTCCCAGGATCACTCTCAAGCCGTGACTCTGATACAAGAGAAAATGACTTTAT
TCAAGAGCCTGATGGATAGATTTGAGCATCATTCGAACATTCTCCTTACCTTTGAAAATAAGGATGAAAATCACTTGCCATTGGTACCACCTAACAAATT
GGAGGAAATGAAAAGACGAATCAACAACATTTTGGAGAAAAAATTTATTCTACTTCTAGAATTTCATTACTACAAGTGCTTAGTTCTTGGTTTGGTAGAT
GAAGTGAAATCAAAATTGGATATTTGGAACATTAAATATGGGAGCAGAGAATCTGTGGAATTATTGCTGGAAGACTGGCATAAATTTATTGAAGAAAAAG
AATTCCTAGCTCGACTTGATACTTCTTTTCAAAAATGTGGAGAAATTTATAAGAATTTGGCTGGAGAATGTCAGAATATTAATAAACAGTATATGATGGT
GAAATCTGATGTTTGTATGTATAGAAAAAATATATATAATGTGAAGTCCACTCTACAAAAAGTGCTGGCATGTTGGGCTACTTATGTGGAAAACCTTCGC
TTACTAAGGGCTTGCTTTGAGGAGACAAAGAAGGAAGAAATTAAAGAGGTACCCTTTGAGACACTAGCCCAGTGGAATCTAGAACACGCTACTTTAAATG
AAGCAGGAAATTTCTTAGTCGAAGTCAGCAATGATGTGGTTGGATCATCTATTTCTAAAGAACTGAGAAGGCTGAATAAAAGATGGAGAAAGTTGGTTTC
AAAAACTCAACTTGAAATGAACCTGCCACTGATGATAAAAAAACAGGATCAGCCCACTTTTGACAATTCTGGAAATATTCTATCTAAAGAAGAGAAAGCA
ACTGTTGAGTTTTCAACAGATATGTCAGTAGAACTTCCTGAAAATTATAATCAAAATATAAAGGCTGGAGAGAAACATGAAAAAGAAAATGAAGAATTCA
CAGGGCAACTAAAAGTGGCTAAAGATGTTGAAAAACTCATTGGACAAGTGGAAATCTGGGAGGCAGAAGCCAAATCTGTTTTGGATCAAGATGATGTGGA
CACCTCAATGGAAGAATCTTTGAAGCATCTTATTGCCAAAGGCTCTATGTTTGATGAGCTTATGGCAAGAAGTGAAGATATGTTACAAATGGATATACAA
AATATTTCAAGCCAGGAGTCCTTTCAACATGTTCTCACAACTGGGCTTCAGGCAAAGATTCAAGAAGCTAAAGAGAAAGTCCAGATCAATGTGGTAAAAC
TCATTGCAGCGTTGAAGAACTTAACTGACGTTTCACCAGATTTGGACATCAGGCTGAAGATGGAAGAATCCCAGAAGGAACTTGAATCATATATGATGAG
GGCTCAGCAGTTACTGGGGCAAAGAGAGAGCCCCGGTGAACTCATTTCAAAACACAAGGAAGCACTAATAATTTCTAATACAAAAAGTCTGGCCAAGTAT
TTGAAAGCTGTTGAAGAACTAAAAAATAATGTAACTGAGGACATAAAGATGTCTTTAGAAGAAAAGAGTAGAGATGTCTGTGCCAAATGGGAGTCTCTTC
ATCATGAACTGTCTTTATATGTTCAACAACTAAAAATAGATATTGAAAAAGGAAAGCTTAGTGACAATATTTTAAAACTTGAAAAGCAAATAAATAAAGA
AAAGAAACTTATCCGTAGAGGAAGGACCAAGGGTCTCATCAAAGAACATGAGGCCTGCTTTTCTGAGGAAGGCTGCCTGTACCAGCTTAATCACCACATG
GAAGTCCTGAGGGAGCTGTGTGAAGAGCTGCCTTCACAGAAGAGTCAACAAGAAGTGAAGAGACTACTCAAAGATTATGAACAAAAGATAGAAAGACTTC
TGAAATGTGCTTCCGAGATTCATATGACACTGCAGCCCACAGCGGGAGGCACGTCGAAAAACGAGGGGACCATCACCACATCTGAGAATAGAGGAGGGGA
TCCCCACAGTGAGGCACCATTTGCAAAATCAGATAATCAGCCATCAACTGAAAAGGCAATGGAACCCACTATGAAGTTTAGCCTGGCATCAGTGTTAAGG
CCTCTGCAAGAAGAAAGCATTATGGAAAAGGATTACAGTGCATCTATAAATAGTTTACTAGAGAGGTATGATACATACAGAGATATTCTTGAACACCACC
TGCAAAACAACAAATTCAGGATTACTTCTGATTTCTCTAGTGAAGAGGACAGGAGTAGTTCTTGTCTGCAGGCTAAACTGACAGATCTACAGGTCATAAA
AAATGAAACTGATGCTCGCTGGAAAGAGTTTGAAATTATTTCATTGAAGTTAGAAAATCATGTGAATGACATAAAAAAGCCTTTTGTAATTAAGGAAAGA
GACACACTAAAGGAAAGAGAAAGAGAGCTTCAGATGACTCTTAATACCAGAATGGAATCTTTAGAGACAGCACTGCGGCTTGTGTTACCTGTAGAGAAGG
CATCACTTCTTCTCTGTGGCTCGGACCTGCCTCTCCATAAAATGGCCATCCAGGGATTTCATCTCATTGATGCTGATCGCATCTATCAACACCTAAGGAA
TATCCAAGATTCCATAGCAAAACAGATTGAAATATGTAACCGCTTAGAAGAGCCAGGCAACTTTGTATTAAAGGAGTTACACCCATTTGATCTACACGCA
ATGCAGAATATTATACTGAAATACAAAACACAATTTGAAGGAATGAACCACAGGGTGCAGAGGAGTGAAGATACTCTCAAAGCTCTGGAAGACTTTTTGG
CGTCTCTCAGAACAGCTAAACTCTCTGCTGAGCCCGTTACAGACCTTTCAGCCTCAGATACACAGGTGGCACAAGAAAATACGTTGACAGTAAAAAATAA
AGAGGGAGAAATTCATCTGATGAAAGACAAGGCCAAACATTTGGATAAATGTTTGAAGATGCTCGATATGAGCTTTAAAGATGCTGAACGGGGTGATGAC
ACCTCCTGTGAAAACCTGCTTGATGCTTTTTCAATAAAGTTATCTGAGACACATGGCTATGGGGTACAGGAGGAATTCACTGAGGAAAACAAATTACTAG
AGGCTTGTATTTTCAAAAATAATGAACTCCTTAAAAATATTCAAGATGTGCAGAGTCAAATCAGTAAAATTGGTCTTAAGGATCCTACTGTTCCAGCTGT
GAAACATCGGAAAAAATCATTAATCAGACTGGATAAGGTTCTAGATGAATATGAAGAAGAGAAGAGACATTTACAAGAAATGGCTAATTCTCTTCCACAC
TTCAAAGATGGCAGAGAAAAAACCGTGAATCAACAGTGCCAAAATACAGTAGTCTTGTGGGAGAATACCAAAGCCTTGGTCACCGAATGTCTTGAACAAT
GTGGGAGAGTTTTGGAGCTCTTAAAACAATATCAGAATTTTAAAAGCATCTTGACAACTTTGATTCAAAAAGAAGAGAGTGTCATCTCCCTGCAGGCTTC
GTACATGGGAAAGGAGAACCTGAAGAAAAGGATAGCAGAGATTGAAATTGTCAAAGAAGAATTTAATGAGCATTTAGAAGTTGTAGACAAGATAAACCAG
GTCTGCAAAAATCTACAATTTTATCTAAATAAAATGAAAACTTTTGAAGAGCCCCCTTTTGAAAAAGAGGCTAATATTATTGTGGATAGATGGCTTGATA
TAAATGAGAAGACAGAAGATTACTATGAAAATCTTGGTCGAGCTCTAGCTTTGTGGGACAAACTTTTTAACTTAAAAAATGTCATTGATGAGTGGACAGA
AAAGGCCCTTCAAAAAATGGAATTACATCAATTGACTGAAGAGGACAGAGAAAGGCTGAAGGAAGAATTACAAGTCCATGAACAAAAAACTTCAGAATTT
TCTAGAAGAGTGGCTGAAATACAGTTTTTGCTCCAAAGCAGTGAAATACCTCTTGAATTGCAGGTCATGGAGTCCTCTATTTTGAACAAGATGGAACATG
TACAGAAGTGCTTAACAGGAGAATCCAACTGCCATGCACTCAGTGGCAGCACTGCTGAGCTAAGGGAGGATCTCGACCAAGCCAAGACCCAGATCGGGAT
GACTGAATCCCTCTTAAAAGCCCTGTCTCCTTCTGACAGCTTGGAGATCTTCACTAAACTAGAGGAGATACAACAGCAGATTCTACAGCAAAAACACAGT
ATGATATTACTTGAGAATCAAATAGGTTGTCTGACTCCTGAACTCTCTGAATTGAAAAAGCAATATGAAAGTGTCAGTGATTTATTTAATACCAAAAAAA
GTGTTTTGCAAGATCACTTTTCTAAGTTATTGAATGATCAATGCAAGAACTTTAATGACTGGTTCAGCAACATTAAAGTGAACCTTAAGGAGTGTTTTGA
ATCATCAGAAACAAAAAAGAGTGTGGAACAAAAGCTACAAAAACTTTCTGATTTCTTGACTCTTGAAGGAAGAAACAGTAAAATAAAGCAGGTGGACAGC
GTACTGAAGCATGTGAAGAAGCATCTGCCCAAAGCACATGTGAAGGAGCTTATCAGTTGGCTCGTGGGTCAGGAATTCGAATTAGAAAAAATGGAGTCCA
TATGCCAGGCTCGAGCAAAGGAGCTTGAAGACTCCTTGCAGCAGCTACTGAGACTCCAGGATGACCATAGAAACCTGAGGAAGTGGTTGACTAATCAAGA
AGAGAAATGGAAAGGAATGGAAGAACCAGGGGAGAAAACTGAGCTGTTCTGCCAAGCTTTAGCTAGAAAGAGGGAACAGTTTGAATCTGTGGCCCAATTG
AACAACTCTTTGAAGGAATATGGGTTTACTGAAGAAGAAGAAATAATAATGGAAGCAACATGTTTGATGGATAGATACCAGACATTACTGAGACAACTAA
GTGAAATCGAGGAAGAGGATAAGTTACTACCCACAGAGGACCAGAGCTTTAATGATCTTGCACATGATGTAATTCATTGGATAAAAGAGATTAAAGAGTC
CCTTATGGTTTTGAATTCATCCGAAGGCAAAATGCCACTTGAGGAAAGAATCCAAAAAATCAAGGAAATCATTTTGCTGAAGCCTGAAGGGGATGCCAGA
ATAGAGACCATCATGAAGCAGGCTGAGAGCAGCGAGGCCCCGCTGGTTCAGAAGACCCTCACTGACATCAGCAACCAGTGGGACAACACACTCCATTTAG
CTAGCACCTACCTAAGCCATCAAGAAAAGCTTCTACTAGAAGGAGAGAAATATTTACAAAGTAAGGAGGATCTGAGATTAATGCTCATAGAACTAAAGAA
GAAACAGGAAGCAGGCTTTGCTCTACAACATGGTCTGCAGGAGAAGAAAGCTCAGTTAAAGATTTATAAGAAATTCCTCAAGAAAGCCCAAGATTTGACA
TCCTTGCTAAAGGAGTTAAAATCTCAGGGAAACTACCTCTTGGAGTGCACTAAAAATCCCAGCTTCAGTGAAGAGCCTTGGCTGGAAATAAAGCATCTAC
ACGAAAGTCTTCTTCAACAACTGCAGGATTCTGTGCAAAACTTGGACGGTCACGTTCGAGAACATGATTCATACCAGGTTTGCGTCACAGACCTGAATAC
TACATTGGACAATTTCTCCAAGGAATTTGTCAGTTTTTCTGATAAGCCTGTGGATCAAATAGCGGTTGAGGAAAAATTGCAGAAACTGCAGGAACTAGAG
AATAGACTCAGTTTACAAGATGGCACATTAAAGAAGATTTTAGCTTTAGCAAAATCCGTCAAGCAAAATACATCTTCAGTGGGGCAGAAGATTATTAAAG
ATGATATAAAATCACTTCAGTGTAAACAAAAAGATTTGGAAAACAGGCTTGCATCTGCTAAGCAGGAGATGGAATGTTGTCTCAACAGCATTCTCAAATC
AAAACGCTCAACAGAAAAGAAAGGAAAGTTTACTCTGCCAGGCAGAGAGAAGCAGGCCACTTCTGATGTGCAGGAGTCTACTCAGGAATCAGCTGCAGTG
GAAAAGTTGGAGGAAGACTGGGAAATAAACAAGGATTCAGCTGTGGAAATGGCTATGTCAAAACAACTTTCTCTTAATGCTCAAGAAAGCATGAAAAACA
CTGAAGATGAGCGGAAAGTCAATGAGCTGCAAAATCAACCTTTAGAATTAGATACTATGTTAAGAAATGAACAATTAGAAGAGATAGAGAAATTATATAC
CCAGTTGGAAGCAAAGAAAGCAGCCATTAAGCCACTGGAACAAACAGAATGTCTTAACAAAACAGAAACTGGGGCCTTGGTTCTCCACAATATAGGATAT
TCGGCACAGCATTTGGACAATTTGCTTCAGGCACTTATTACTTTGAAGAAAAACAAAGAAAGCCAATATTGTGTCCTCAGAGATTTTCAGGAATACCTTG
CTGCAGTTGAATCTTCAATGAAAGCCTTGTTGACAGACAAGGAAAGTCTTAAAGTAGGACCACTGGACAGTGTAACGTATCTGGACAAAATTAAAAAATT
CATAGCATCCATAGAAAAAGAGAAAGATTCTTTAGGCAACTTGAAAATCAAATGGGAGAATTTATCAAACCACGTGACTGACATGGATAAGAAATTGTTG
GAAAGCCAGATTAAGCAACTTGAACATGGTTGGGAACAAGTGGAACAGCAGATTCAAAAGAAGTATTCTCAGCAGGTAGTGGAATATGATGAATTTACAA
CCCTCATGAATAAGGTACAGGACACTGAGATTTCTCTGCAACAGCAGCAGCAACATCTACAGTTAAGGCTGAAGTCTCCAGAAGAACGGGCAGGGAACCA
AAGCATGATTGCCTTGACCACTGACCTCCAGGCTACCAAGCATGGATTTTCTGTTTTAAAGGGGCAAGCTGAACTTCAGATGAAGAGGATTTGGGGAGAA
AAAGAAAAGAAGAATTTGGAGGATGGAATAAATAACTTGAAGAAACAATGGGAAACATTGGAGCCATTACACTTAGAAGCAGAAAATCAGATTAAGAAGT
GTGACATAAGGAACAAGATGAAAGAGACTATCTTATGGGCCAAGAATTTGTTGGGTGAACTTAATCCCTCCATTCCCCTTCTCCCAGATGACATTCTTTC
ACAGATCAGAAAGTGCAAAGTGACACATGATGGCATTCTAGCTAGGCAGCAGTCTGTGGAATCGTTGGCTGAAGAGGTCAAAGATAAGGTTCCTAGCCTT
ACAACCTATGAGGGCAGTGATTTAAATAATACCCTAGAGGACTTACGGAATCAATACCAAATGCTGGTTTTAAAATCAACTCAAAGATCACAGCAATTAG
AATTTAAGTTGGAAGAAAGAAGCAATTTTTTTGCTATAATAAGGAAGTTTCAACTTATGGTTCAAGAAAGTGAAACACTGATAATTCCCAGGGTGGAGAC
AGCTGCCACGGAAGCTGAACTAAAACATCACCATGTTACTTTGGAGGCATCTCAGAAGGAATTGCAAGAAATTGACAGTGGAATCTCAACACATCTTCAG
GAGCTAACAAACATCTATGAGGAGCTGAATGTGTTTGAAAGATTATTTCTGGAAGATCAGTTGAAAAATCTTAAGATTAGGACCAACAGAATACAAAGAT
TCATTCAGAATACATGTAATGAAGTGGAACACAAGATAAAGTTTTGCAGACAATTCCATGAAAAAACATCAGCGCTTCAGGAGGAGGCTGACAGTATACA
GCGCAATGAACTATTACTTAATCAAGAAGTAAATAAAGGTGTTAAAGAGGAGATCTATAATCTTAAAGACAGACTCACCGCTATTAAGTGTTGCATCTTA
CAGGTATTGAAACTTAAAAAAGTGTTTGACTATATTGGACTAAACTGGGATTTTTCACAACTTGACCAATTACAAACCCAAGTATTTGAAAAAGAAAAGG
AACTTGAAGAAAAAATTAAGCAGTTGGACACATTTGAGGAAGAACATGGCAAATATCAGGCATTATTAAGTAAAATGAGAGCTATTGATTTGCAAATTAA
GAAAATGACTGAAGTAGTACTAAAAGCTCCTGATAGCTCTCCGGAAAGCAGACGGCTCAATGCCCAAATTTTAAGTCAGAGAATTGAGAAAGCCAAGTGT
TTATGTGATGAGATAATAAAGAAATTAAATGAAAATAAGACCTTTGATGACTCATTCAAGGAGAAAGAAATACTACAAATAAAGCTGAATGCAGAAGAAA
ATGATAAGTTATACAAAGTTCTCCAAAACATGGTATTAGAACTCTCACCAAAAGAATTGGATGAAAAGAATTGTCAGGACAAACTAGAAACTTCCTTACA
TGTTTTAAATCAGATAAAATCTCAATTACAGCAGCCATTACTTATAAATTTGGAAATTAAACATATTCAAAATGAAAAGGACAATTGTGAAGCATTTCAG
GAGCAAGTTTGGGCAGAAATGTGTAGTATTAAAGCTGTGACTGCTATTGAGAAACAAAGAGAAGAAAACTCTTCTGAAGCGAGTGATGTGGAGACAAAAC
TACGTGAGTTTGAAGATCTTCAGATGCAGCTTAACACAAGCATTGATTTGCGCACAAATGTCTTGAATGATGCTTATGAAAATCTAACACGCTATAAAGA
AGCAGTCACCAGGGCAGTGGAGAGCATCACTTCCCTCGAAGCCATCATTATACCCTACAGAGTAGATGTTGGTAATCCAGAAGAATCTTTAGAGATGCCT
CTTCGAAAACAAGAGGAATTGGAATCCACAGTAGCACACATCCAGGACCTCACTGAGAAACTGGGAATGATATCCAGCCCCGAAGCCAAACTACAACTTC
AGTATACTTTACAGGAACTAGTTTCTAAGAACTCAGCAATGAAGGAAGCTTTCAAAGCACAGGAAACTGAGGCAGAAAGGTATCTTGAGAATTACAAATG
CTATAGAAAAATGGAAGAGGATATTTACACTAACCTCAGCAAAATGGAGACAGTTCTTGGACAGTCCATGTCCTCGTTGCCACTGTCTTACAGAGAAGCT
TTAGAGCGCTTGGAACAGAGCAAGGCCTTGGTGTCAAATCTTATATCAACCAAAGAAGAGTTAATGAAACTACGACAGATCCTTAGACTCTTGAGACTCA
GGTGCACAGAAAATGATGGCATATGTTTGCTCAAGATTGTGTCGGCTCTGTGGGAGAAATGGCTGAGTTTGCTGGAAGCTGCTAAAGAGTGGGAGATGTG
GTGCGAAGAACTGAAGCAGGAATGGAAATTTGTCAGTGAAGAAATTGAACGAGAGGCAATTATTTTAGATAATCTTCAGGAAGAACTCCCTGAAATTTCC
AAAACAAAAGAGGCAGCCACCACAGAGGAACTCTCTGAGCTGCTAGACTGTTTATGCCAATATGGAGAGAACGTGGAGAAGCAACAGCTGTTACTGACTC
TACTTCTTCAGCGCATCAGAAGTATCCAGAATGTTCCTGAAAGCTCAGGGGCTGTGGAAACTGTTCCAGCATTTCAAGAAATTACTTCTATGAAAGAACG
ATGCAACAAGCTTCTTCAGAAAGTTCAGAAAAATAAAGAATTGGTGCAGACTGAAATCCAAGAAAGACATTCCTTCACAAAAGAGATAATTGCTTTGAAG
AATTTCTTTCAACAGACCACAACTTCATTCCAAAATATGGCATTCCAGGATCACCCAGAAAAGTCAGAACAATTTGAGGAGCTTCAAAGCATCCTTAAGA
AAGGGAAACTAACTTTTGAGAATATTATGGAAAAACTGCGAATCAAGTATTCCGAAATGTACACCATAGTCCCTGCAGAGATTGAATCCCAGGTGGAAGA
ATGCAGAAAAGCTTTAGAAGACATAGATGAGAAGATTAGCAATGAAGTCTTAAAAAGCTCACCATCATATGCAATGAGGAGAAAAATAGAAGAAATTAAC
AATGGGCTTCATAATGTTGAAAAGATGTTGCAGCAGAAAAGCAAAAATATTGAGAAAGCTCAAGAAATTCAAAAGAAAATGTGGGACGAGTTAGATCTAT
GGCATTCCAAACTAAATGAGCTGGATTCTGAAGTTCAGGACATTGTTGAACAGGACCCAGGACAGGCTCAAGAATGGATGGATAACTTGATGATTCCTTT
CCAGCAGTATCAGCAAGTATCACAGAGAGCAGAGTGTAGAACCTCACAGTTGAATAAGGCCACAGTTAAGATGGAGGAATATAGTGACCTTCTGAAGAGC
ACTGAGGCTTGGATAGAAAATACCAGTCATTTGCTGGCCAATCCTGCTGACTATGACTCTTTGAGGACACTGAGTCACCATGCTAGCACTGTGCAGATGG
CTTTGGAAGATTCAGAACAGAAGCACAATCTTTTACATTCAATCTTTATGGATCTAGAAGACCTGTCAATAATTTTTGAAACAGATGAATTAACCCAATC
CATACAAGAGTTAAGTAATCAAGTAACAGCTTTACAACAAAAAATAATGGAAAGCCTTCCACAGATTCAGCGAATGGCTGATGATGTGGTTGCTATTGAA
TCTGAAGTAAAATCAATGGAAAAAAGAGTTTCAAAAATCAAAACTATCCTATTATCAAAAGAAATATTTGATTTTTCACCTGAAGAACATCTCAAACATG
GGGAGGTCATACTTGAAAATATACGTCCCATGAAGAAAACCATTGCTGAGATAGTGTCTTACCAAGTGGAACTGAGGTTGCCCCAAACAGGAATGAAACC
TCTGCCTGTGTTTCAGCGGACAAATCAGCTTTTACAAGATATAAAACTATTGGAAAATGTGACTCAAGAACAAAATGAGTTATTAAAGGTAGTCATAAAA
CAGACCAATGAATGGGATGAAGAAATAGAAAATTTGAAACAGATCTTAAATAATTATTCAGCTCAGTTCTCCCTTGAACATATGTCACCAGACCAAGCTG
ACAAGCTGCCACAACTACAGGGAGAAATCGAACGTATGGAGAAACAGATTCTGAGTTTGAACCAGAGAAAAGAAGACCTGTTGGTGGACTTGAAGGCCAC
CGTACTAAACCTTCACCAGCATTTGAAGCAAGAACAAGAAGGAGTAGAAAGAGATAGGCTGCCAGCTGTAACATCAGAGGAAGGTGGAGTGGCAGAGAGG
GATGCTTCTGAGCGGAAGTTGAACAGAAGAGGCTCCATGTCTTACCTGGCAGCAGTCGAGGAAGAGGTGGAAGAAAGTTCCGTGAAGAGCGATAATGGAG
ATGAGAAGGCAGAGCCATCGCCTCAGTCTTGGTCTTCACTTTGGAAGCATGACAAGGACATGGAAGAAGACAGAGCTTCCTCATCCTCTGGAACAATTGT
TCAGGAAGCATATGGGAAAATAAGCACCTCTGATAATTCCATGGCACAAATCCTCACACCAGACTCACTAAACACTGAGCAAGGCCCAGAATGTTCCCTA
AGGCCCAACCAAACAGAAGAGGGCACCACACCTCCTATTGAGGCTGACACTCTGGACTCTTCTGACGCGCAAGGAGGTTTGGAGCCCAGGGTGGAGAAAA
CTAGGCCGGAGCCCACAGAAGTCCTGCATGCCTGCAAGACCCAGGTGGCCGAGCTGGAGCTGTGGCTGCAACAAGCCAACGTGGCAGTTGAGCCGGAAAC
ATTAAACGCAGACATGCAGCAGGTGCTGGAACAGCAGCTGGTAGGGTGCCAGGCTATGCTAACAGAGATTGAGCACAAGGTTGCCTTTCTGTTAGAGACT
TGCAAAGATCAGGGCCTGGGAGATAATGGAGCCACTCAACATGAGGCTGAAGCGCTTTCCCTGAAACTGAAAACAGTGAAGTGCAATTTAGAAAAAGTCC
AGATGATGCTTCAGGAGAAGCACAGTGAAGATCAGCATCCTACCATTCTAAAGAAATCCTCAGAGCCAGAGCATCAAGAAGCTCTCCAACCAGTTAACCT
TTCTGAATTGGAATCCATTGTAACTGAAAGGCCACAATTCAGCAGACAAAAAGATTTCCAGCAGCAACAGGTTCTGGAGTTAAAACCAATGGAACAGAAA
GATTTCATCAAATTCATAGAATTTAATGCTAAGAAAATGTGGCCCCAGTATTGCCAACATGATAACGATACAACTCAGGAATCATCTGCAAGCAACCAGG
CATCCAGCCCTGAAAATGACGTTCCAGACTCGATCTTGTCACCCCAGGGCCAAAATGGAGATAAGTGGCAATATCTGCATCATGAACTCTCATCAAAAAT
AAAGCTCCCACTCCCTCAGCTTGTGGAGCCTCAGGTTTCCACAAATATGGGTATTCTACCCAGCGTGACTATGTATAACTTTAGATACCCAACAACTGAA
GAACTGAAAACCTATACCACCCAACTTGAAGACCTGCGCCAAGAAGCAAGTAACCTTCAGACACAGGAAAATATGACAGAAGAAGCATATATCAATTTGG
ATAAAAAATTGTTTGAACTATTCCTGACCCTCAGTCAGTGCCTCAGCAGTGTGGAGGAGATGCTGGAGATGCCCAGACTTTACAGGGAGGATGGTTCTGG
CCAGCAGGTGCACTACGAGACGCTGGCTCTTGAGTTGAAGAAACTTTATTTAGCGCTAAGTGACAAGAAGGGTGATCTTTTGAAAGCCATGACTTGGCCT
GGCGAGAACACCAACTTGCTCCTTGAATGTTTTGACAACCTTCAAGTCTGCCTGGAGCACACTCAGGCTGCAGCTGTCTGTAGAAGCAAGTCCCTGAAAG
CTGGCCTCGATTACAACCGCAGTTACCAGAATGAAATAAAGAGATTATATCATCAGCTCATTAAGAGTAAGACATCTTTACAACAGTCTTTGAATGAAAT
CAGTGGGCAGAGTGTTGCTGAACAGCTTCAGAAAGCAGATGCATATACAGTGGAGCTGGAGAACGCCGAGAGCCGAGTGGCCAAACTAAGAGATGAAGGG
GAGAGGCTTCATTTACCTTATGCTTTACTCCAGGAGGTTTACAAATTAGAGGATGTACTTGACAGTATGTGGGGAATGCTAAGAGCCAGGTACACAGAAC
TCAGCAGCCCTTTCGTCACTGAGAGCCAGCAAGATGCTTTGTTGCAAGGCATGGTGGAACTGGTGAAGATTGGGAAGGAAAAGCTTGCTCATGGCCACTT
AAAACAAACCAAAAGTAAAGTGGCGTTACAGGCTCAAATAGAAAATCACAAGGTTTTTTTCCAGAAGCTTGTTGCTGACATGTTGTTGATCCAAGCATAC
TCTGCCAAAATACTTCCTTCTTTATTGCAAAACAGAGAGACATTTTGGGCAGAACAAGTAACAGAAGTTAAAATACTAGAAGAAAAGTCACGCCAATGTG
GTATGAAGCTGCAGAGTTTGTTGCAGAAATGGGAAGAATTTGATGAAAACTATGCATCTCTTGAAAAGGACCTGGAAATTCTTATATCTACATTGCCCTC
TGTGAGTTTGGTGGAAGAAACAGAGGAAAGATTAGTGGAAAGGATTTCATTTTACCAGCAAATAAAAAGAAACATTGGTGGAAAACACGCCCGGCTTTAC
CAAACTCTGAACGAAGGCAAACAGTTGGTGGCGTCTGTGAGCTGTCCTGAATTAGAGGGCCAGATCGCAAAACTGGAAGAGCAGTGGTTGTCCCTGAACA
AGAAAATTGACCATGAGCTCCACAGGCTGCAAGCTCTTCTCAAGCATCTGCTCAGTTATAACAGAGATTCGGATCAGTTAACCAAGTGGTTGGAATCTTC
CCAGCATACTCTGAATTACTGGAAAGAACAGTCCCTCAATGTGTCTCAGGACTTGGATACAATCAGAAGCAACATCAACAATTTTTTTGAGTTTTCAAAA
GAAGTTGATGAAAAATCCTCCTTGAAGACTGCCGTTATCAGTATCGGGAACCAGCTTCTTCACCTGAAAGAAACTGATACAGCTACACTGAGAGCTTCTT
TAGCACAGTTTGAACAAAAATGGACAATGCTCATAACTCAACTTCCAGATATTCAAGAAAAACTTCACCAGCTTCAAATGGAGAAATTGCCGTCTCGTAA
AGCAATCACAGAAATGATTAGCTGGATGAACAATGTGGAGCATCAAACTTCAGATGAAGACTCCGTGCATTCACCAAGTTCTGCATCTCAAGTTAAACAT
CTTCTTCAGAAGCACAAGGAGTTTAGAATGGAAATGGACTATAAACAGTGGATAGTTGACTTCGTTAACCAGTCATTACTTCAGCTAAGCACCTGTGATG
TAGAAAGCAAGCGCTATGAAAGAACGGAGTTTGCAGAGCACCTGGGGGAGATGAACCGCCAGTGGCACCGTGTACATGGAATGCTGAATAGAAAGATACA
ACATTTAGAACAACTTCTAGAAAGTATCACTGAGAGTGAAAATAAAATACAGATCTTGAACAACTGGCTGGAAGCACAAGAAGAGAGACTGAAAACTTTA
CAAAAACCTGAAAGTGTGATCTCAGTGCAGAAGCTGCTCCTGGACTGTCAGGATATAGAAAATCAACTTGCAATTAAATCCAAAGCACTAGATGAGTTGA
AACAAAGTTATCTGACTTTGGAGAGTGGGGCAGTGCCATTGTTAGAAGATACAGCATCCCGAATTGATGAGTTATTTCAAAAGAGAAGCAGTGTTCTCAC
TCAGGTCAATCAGCTCAAAACCTCCATGCAGTCAGTTTTACAGGAGTGGAAGATTTATGATCAACTCTATGATGAAGTGAATATGATGACAATCCGATTC
TGGTACTGCATGGAACACAGCAAGCCTGTGGTGTTATCATTGGAGACCTTGAGATGCCAGGTGGAGAACCTTCAGTCTCTGCAAGATGAAGCTGAGAGCA
GTGAAGGGAGTTGGGAGAAACTCCAGGAGGTTATCGGCAAACTCAAAGGTCTCTGCCCCTCTGTTGCTGAAATAATCGAAGAGAAATGCCAAAATACTCA
TAAAAGGTGGACTCAGGTGAACCAAGCCATTGCAGACCAGTTGCAGAAGGCCCAGAGTCTGCTCCAGCTCTGGAAGGCCTATAGCAATGCTCATGGTGAA
GCTGCCGCAAGGCTGAAGCAGCAGGAAGCAAAGTTTCAACAGCTCGCAAACATCAGCATGTCTGGAAACAACCTGGCAGAGATCCTGCCCCCAGCCCTGC
AGGACATAAAGGAGCTGCAGCATGATGTGCAGAAAACAAAAGAAGCCTTTCTCCAAAATTCCAGTGTCCTGGATCGACTCCCACAACCCGCAGAGTCCAG
CACCCACATGCTCCTCCCGGGCCCCCTGCACTCTCTCCAGAGGGCTGCTTATTTGGAAAAGATGCTGCTTGTGAAAGCAAATGAATTTGAGTTTGTTCTC
TCACAGTTTAAGGATTTTGGAGTCCGGCTGGAATCTTTAAAAGGTCTTATTATGCATGAAGAAGAGAATTTGGATAGACTTCACCAACAGGAAAAAGAAA
ATCCTGACTCATTCCTGAATCATGTGCTGGCACTGACAGCCCAATCACCTGATATTGAACATTTGAATGAAGTGAGCCTCAAGCTCCCACTTAGTGACGT
AGCTGTGAAGACGTTACAAAATATGAACCGGCAATGGATTCGGGCCACGGCCACGGCACTGGAGCGCTGCAGTGAGCTTCAGGGAATTGGATTGAATGAA
AAGTTTCTTTATTGCTGTGAAAAGTGGATCCAACTTTTGGAGAAGATAGAAGAAGCACTCAAAGTGGATGTGGCTAACAGCCTTCCTGAGCTCCTGGAGC
AGCAGAAAACCTATAAGATGTTAGAAGCTGAAGTTTCTATAAACCAGACAATTGCTGATTCCTATGTCACCCAGTCCTTACAACTCCTGGACACAACAGA
AATAGAGAACAGACCAGAATTTATTACAGAATTCTCAAAGCTGACGGATCGGTGGCAGAATGCTGTCCAGGGTGTTCGGCAGAGGAAGGGTGACGTTGAT
GGGCTGGTGAGGCAGTGGCAAGATTTCACTACTTCTGTGGAGAACTTGTTTCGCTTCCTCACTGACACCAGCCACCTGCTATCTGCAGTGAAGGGCCAGG
AGCGCTTCAGCCTCTACCAAACCAGAAGTCTGATCCATGAGCTGAAGAATAAAGAAATTCATTTTCAAAGGAGGCGAACTACCTGTGCCCTAACCTTGGA
AGCTGGAGAAAAGTTACTGCTCACAACTGACCTGAAAACTAAAGAGTCTGTGGGTAGGAGAATCAGTCAACTTCAGGACAGCTGGAAAGACATGGAGCCC
CAGCTGGCAGAGATGATTAAGCAGTTCCAGAGCACTGTAGAGACCTGGGACCAGTGTGAAAAGAAAATCAAGGAGTTGAAAAGCAGGCTGCAAGTTTTAA
AGGCACAAAGTGAAGATCCTCTTCCAGAGCTTCACGAGGACCTCCATAACGAAAAAGAGCTGATTAAGGAACTAGAACAGTCTTTGGCTAGCTGGACTCA
GAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGTTCTCGTGGAAGATGTGATGGTTTTGAAGGAGCAAATAGAGCATTTGCACAGA
CAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATTGAAGACAGACTCAATACATGGGTTGTATTCAATGAAAAAAATAAAGAGTTGT
GTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACATTAGTATTGAAGAAATGATTGAAAAGTTACAGAAGGACTGCATGGAAGAAAT
AAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCAGTTGATCAAGGCCAGCAACAAATCAAGAGCAGCTGAGATCGATGACAAGCTC
AACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCAAGGGTGAAGAAGCTGAAGGAGACCTTTGCTTTTATTCAGCAGTTGGACAAAA
ACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGATGATCAAGAGATCCAGAAGAGGCT
CGCTGAGCAGCAGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGACGTCCTACTGCACGACTCCGATGCC
TGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTGCCATGTCCATGGAGCGGCGCATGA
AAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGCTGAGAGGACGGCAGCCTGCCCAAA
TTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAGCGGCTCACTCAGCTGGAGCTCATC
AACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGGGCAACCAGCGCTGGGACAACCTTC
AGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGAGAGCATTCTGGTGTGGCTCACAGA
GATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGCTTCCAACAGGAAATTACATTAAAT
ACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGATTGAGGATGAGCTGGAGGAACTCC
ACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGAAGATGAAAAGGAGGCCTCTGAGAA
TGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCATCTA
GTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCTCCT
CTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGGTAAATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCC
CGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTA
CCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAG
GTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCAT
CACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTG
CAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAG
AGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTG
CCAGGACTTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCC
CGGGCTCTCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCC
TTCTCATTAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGA
TTTAATGGCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCT
CCCCGAGCAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCT
CAAGGGTGGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCAC
TCAGGCCAACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCAC
CTGCCTGATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTT
ACCCAGCACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGA
ACTAGTTGATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATG
TAGGTATGGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTA
GCTGATGGAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGT
TCAGTACCTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATC
TGTATGAATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAG
GGATGTTTTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGG
GGTTGGTACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
AKERSALSCAQSLRPDLKKREGERAKGAIQRARQAGRRPRAAPARALAPRPGGRSLLQLRWT*YNLH
*VKEWHLVLSFPPKMNRVPGASTISIFHCKLNR
KTPRRKPSRAG*THSWPGTLLPQLYPTYSQTLK
RGMSSWIC*KYFLGNSCLGIKDLIPSSVESI*NM
P*HS*ETDQLS**IFMLLISLMETHPLSLA*FG
QLSCTFILRSLPRLFLAITISLPWMM*VWLTHL
LPQVLQLRNALKCKQDGKCLQERPFFCGLRNNAP
PMSLSM*PILSQVGEMGWLFWPSFMPCDQT*LT
*RV*SIDPTKTI*ERPSELQNKN*KSPDCWNQK
MWMLLILMKSPS*PMWHSFCSIPKMPLGLERRLR
ER*KMLWAG*LCKRKNYRSC*RIQRMIPTLKSI
IACCPLWSHSMKKKSPFWMSCQ*NGIWMSWTRI
IYS*EKPGMASITRLMHGK*S*IMPCPHPSIKLK
LGSRR*KSLWMKICQPPRITLKP*L*YKRK*LY
SRA*WIDLSIIRTFSLPLKIRMKITCHWYHLTN
WRK*KDESTTFWRKNLFYF*NFITTSA*FLVW*M
K*NQNWIFGTLNMGAENLWNYCWKTGINLLKKK
NS*LDLILLFKNVEKFIRIWLENVRILINSI*W
*NLMFVCIEKIYIM*SPLYKKCWHVGLLMWKTFA
Y*GLALRRQRRKKLKRYPLRH*PSGI*NTLL*M
KQEIS*SKSAMMWLDHLFLKN*EG*IKDGESWF
QKLNLK*TCH***KNRISPLLTILEIFYLKKRKQ
LLSFQQICQ*NFLKIIIKI*RLERNMKKKMKNS
QGN*KWLKMLKNSLDKWKSGRQKPNLFWIKMMW
TPQWKNL*SILLPKALCLMSLWQEVKICYKWIYK
IFQARSPFNMFSQLGFRQRFKKLKRKSRSMW*N
SLQR*RT*LTFHQIWTSG*RWKNPRRNLNHI**
GLSSYWGKERAPVNSFQNTRKH**FLIQKVWPSI
*KLLKN*KIM*LRT*RCL*KKRVEMSVPNGSLF
IMNCLYMFNN*K*ILKKESLVTIF*NLKSK*IK
KRNLSVEEGPRVSSKNMRPAFLRKAACTSLITTW
KS*GSCVKSCLHRRVNKK*RDYSKIMNKR*KDF
*NVLPRFI*HCSPQREARRKTRGPSPHLRIEEG
IPTVRHHLQNQIISHQLKRQWNPL*SLAWHQC*G
LCKKKALWKRITVHL*IVY*RGMIHTEIFLNTT
CKTTNSGLLLISLVKRTGVVLVCRLN*QIYRS*
KMKLMLAGKSLKLFH*S*KIM*MT*KSLL*LRKE
TH*RKEKESFR*LLIPEWNL*RQHCGLCYL*RR
HHFFSVARTCLSIKWPSRDFISLMLIASINT*G
ISKIP*QNRLKYVTA*KSQATLY*RSYTHLIYTQ
CRILY*NTKHNLKE*TTGCRGVKILSKLWKTFW
RLSEQLNSLLSPLQTFQPQIHRWHKKIR*Q*KI
KREKFI**KTRPNIWINV*RCSI*ALKMLNGVMT
PPVKTCLMLFQ*SYLRHMAMGYRRNSLRKTNY*
RLVFSKIMNSLKIFKMCRVKSVKLVLRILLFQL
*NIGKNH*SDWIRF*MNMKKRRDIYKKWLILFHT
SKMAEKKP*INSAKIQ*SCGRIPKPWSPNVLNN
VGEFWSS*NNIRILKAS*QL*FKKKRVSSPCRL
RTWERRT*RKG*QRLKLSKKNLMSI*KL*TR*TR
SAKIYNFI*IK*KLLKSPLLKKRLILLWIDGLI
*MRRQKITMKILVEL*LCGTNFLT*KMSLMSGQ
KRPFKKWNYIN*LKRTEKG*RKNYKSMNKKLQNF
LEEWLKYSFCSKAVKYLLNCRSWSPLF*TRWNM
YRSA*QENPTAMHSVAALLS*GRISTKPRPRSG
*LNPS*KPCLLLTAWRSSLN*RRYNSRFYSKNTV
*YYLRIK*VV*LLNSLN*KSNMKVSVIYLIPKK
VFCKITFLSY*MINARTLMTGSATLK*TLRSVL
NHQKQKRVWNKSYKNFLIS*LLKEETVK*SRWTA
Y*SM*RSICPKHM*RSLSVGSWVRNSN*KKWSP
YARLEQRSLKTPCSSY*DSRMTIET*GSG*LIK
KRNGKEWKNQGRKLSCSAKL*LERGNSLNLWPN*
TTL*RNMGLLKKKK**WKQHV*WIDTRHY*DN*
VKSRKRISYYPQRTRALMILHMM*FIG*KRLKS
PLWF*IHPKAKCHLRKESKKSRKSFC*SLKGMPE
*RPS*SRLRAARPRWFRRPSLTSATSGTTHSI*
LAPT*AIKKSFY*KERNIYKVRRI*D*CS*N*R
RNRKQALLYNMVCRRRKLS*RFIRNSSRKPKI*H
PC*RS*NLRETTSWSALKIPASVKSLGWK*SIY
TKVFFNNCRILCKTWTVTFENMIHTRFASQT*I
LHWTISPRNLSVFLISLWIK*RLRKNCRNCRN*R
IDSVYKMAH*RRF*L*QNPSSKIHLQWGRRLLK
MI*NHFSVNKKIWKTGLHLLSRRWNVVSTAFSN
QNAQQKRKESLLCQAERSRPLLMCRSLLRNQLQW
KSWRKTGK*TRIQLWKWLCQNNFLLMLKKA*KT
LKMSGKSMSCKINL*N*ILC*EMNN*KR*RNYI
PSWKQRKQPLSHWNKQNVLTKQKLGPWFSTI*DI
RHSIWTICFRHLLL*RKTKKANIVSSEIFRNTL
LQLNLQ*KPC*QTRKVLK*DHWTV*RIWTKLKN
S*HP*KKRKIL*AT*KSNGRIYQTT*LTWIRNCW
KARLSNLNMVGNKWNSRFKRSILSR*WNMMNLQ
PS*IRYRTLRFLCNSSSNIYS*G*SLQKNGQGT
KA*LP*PLTSRLPSMDFLF*RGKLNFR*RGFGEK
KKRRIWRME*IT*RNNGKHWSHYT*KQKIRLRS
VT*GTR*KRLSYGPRICWVNLIPPFPFSQMTFF
HRSESAK*HMMAF*LGSSLWNRWLKRSKIRFLAL
QPMRAVI*IIP*RTYGINTKCWF*NQLKDHSN*
NLSWKKEAIFLL**GSFNLWFKKVKH**FPGWR
QLPRKLN*NITMLLWRHLRRNCKKLTVESQHIFR
S*QTSMRS*MCLKDYFWKIS*KILRLGPTEYKD
SFRIHVMKWNTR*SFADNSMKKHQRFRRRLTVY
SAMNYYLIKK*IKVLKRRSIILKTDSPLLSVASY
RY*NLKKCLTILD*TGIFHNLTNYKPKYLKKKR
NLKKKLSSWTHLRKNMANIRHY*VK*ELLICKL
RK*LK*Y*KLLIALRKADGSMPKF*VRELRKPSV
YVMR**RN*MKIRPLMTHSRRKKYYK*S*MQKK
MISYTKFSKTWY*NSHQKNWMKRIVRTN*KLPY
MF*IR*NLNYSSHYL*IWKLNIFKMKRTIVKHFR
SKFGQKCVVLKL*LLLRNKEKKTLLKRVMWRQN
YVSLKIFRCSLTQALICAQMS*MMLMKI*HAIK
KQSPGQWRASLPSKPSLYPTE*MLVIQKNL*RCL
FENKRNWNPQ*HTSRTSLRNWE*YPAPKPNYNF
SILYRN*FLRTQQ*RKLSKHRKLRQKGILRITN
AIEKWKRIFTLTSAKWRQFLDSPCPRCHCLTEKL
*SAWNRARPWCQILYQPKKS**NYDRSLDS*DS
GAQKMMAYVCSRLCRLCGRNG*VCWKLLKSGRC
GAKN*SRNGNLSVKKLNERQLF*IIFRKNSLKFP
KQKRQPPQRNSLSC*TVYANMERTWRSNSCY*L
YFFSASEVSRMFLKAQGLWKLFQHFKKLLL*KN
DATSFFRKFRKIKNWCRLKSKKDIPSQKR*LL*R
ISFNRPQLHSKIWHSRITQKSQNNLRSFKASLR
KGN*LLRILWKNCESSIPKCTP*SLQRLNPRWK
NAEKL*KT*MRRLAMKS*KAHHHMQ*GEK*KKLT
MGFIMLKRCCSRKAKILRKLKKFKRKCGTS*IY
GIPN*MSWILKFRTLLNRTQDRLKNGWIT**FL
SSSISKYHREQSVEPHS*IRPQLRWRNIVTF*RA
LRLG*KIPVICWPILLTMTL*GH*VTMLALCRW
LWKIQNRSTIFYIQSLWI*KTCQ*FLKQMN*PN
PYKS*VIK*QLYNKK*WKAFHRFSEWLMMWLLLN
LK*NQWKKEFQKSKLSYYQKKYLIFHLKNISNM
GRSYLKIYVP*RKPLLR*CLTKWN*GCPKQE*N
LCLCFSGQISFYKI*NYWKM*LKNKMSY*R*S*N
RPMNGMKK*KI*NRS*IIIQLSSPLNICHQTKL
TSCHNYREKSNVWRNRF*V*TREKKTCWWT*RP
PY*TFTSI*SKNKKE*KEIGCQL*HQRKVEWQRG
MLLSGS*TEEAPCLTWQQSRKRWKKVP*RAIME
MRRQSHRLSLGLHFGSMTRTWKKTELPHPLEQL
FRKHMGK*APLIIPWHKSSHQTH*TLSKAQNVP*
GPTKQKRAPHLLLRLTLWTLLTRKEVWSPGWRK
LGRSPQKSCMPARPRWPSWSCGCNKPTWQLSRK
H*TQTCSRCWNSSW*GARLC*QRLSTRLPFC*RL
AKIRAWEIMEPLNMRLKRFP*N*KQ*SAI*KKS
R*CFRRSTVKISILPF*RNPQSQSIKKLSNQLT
FLNWNPL*LKGHNSADKKISSSNRFWS*NQWNRK
ISSNS*NLMLRKCGPSIANMITIQLRNHLQATR
HPALKMTFQTRSCHPRAKMEISGNICIMNSHQK
*SSHSLSLWSLRFPQIWVFYPA*LCITLDTQQLK
N*KPIPPNLKTCAKKQVTFRHRKI*QKKHISIW
IKNCLNYS*PSVSASAVWRRCWRCPDFTGRMVL
ASRCTTRRWLLS*RNFI*R*VTRRVIF*KP*LGL
ARTPTCSLNVLTTFKSAWSTLRLQLSVEASP*K
LASITTAVTRMK*RDYIISSLRVRHLYNSL*MK
SVGRVLLNSFRKQMHIQWSWRTPRAEWPN*EMKG
RGFIYLMLYSRRFTN*RMYLTVCGEC*EPGTQN
SAALSSLRASKMLCCKAWWNW*RLGRKSLLMAT
*NKPKVKWRYRLK*KITRFFSRSLLLTCC*SKHT
LPKYFLLYCKTERHFGQNK*QKLKY*KKSHANV
V*SCRVCCRNGKNLMKTMHLLKRTWKFLYLHCP
L*VWWKKQRKD*WKGFHFTSK*KETLVENTPGFT
KL*TKANSWWRL*AVLN*RARSQNWKSSGCP*T
RKLTMSSTGCKLFSSICSVITEIRIS*PSGWNL
PSIL*ITGKNSPSMCLRTWIQSEATSTIFLSFQK
KLMKNPP*RLPLSVSGTSFFT*KKLIQLH*ELL
*HSLNKNGQCS*LNFQIFKKNFTSFKWRNCRLV
KQSQK*LAG*TMWSIKLQMKTPCIHQVLHLKLNI
FFRSTRSLEWKWTINSG*LTSLTSHYFS*APVM
*KASAMKERSLQSTWGR*TASGTVYMEC*IERY
NI*NNF*KVSLRVKIKYRS*TTGWKHKKRD*KLY
KNLKV*SQCRSCSWTVRI*KINLQLNPKH*MS*
NKVI*LWRVGQCHC*KIQHPELMSYFKREAVFS
LRSISSKPPCSQFYRSGRFMINSMMK*I**QSDS
GTAWNTASLWCYHWRP*DARWRTFSLCKMKLRA
VKGVGRNSRRLSANSKVSAPLLLK*SKRNAKIL
IKGGLR*TKPLQTSCRRPRVCSSSGRPIAMLMVK
LPQG*SSRKQSFNSSQTSACLETTWQRSCPQPC
RT*RSCSMMCRKQKKPFSKIPVSWIDSHNPQSP
APTCSSRAPCTLSRGLLIWKRCCL*KQMNLSLFS
HSLRILESGWNL*KVLLCMKKRIWIDFTNRKKK
ILTHS*IMCWH*QPNHLILNI*MK*ASSSHLVT
*L*RRYKI*TGNGFGPRPRHWSAAVSFRELD*MK
SFFIAVKSGSNFWRR*KKHSKWMWLTAFLSSWS
SRKPIRC*KLKFL*TRQLLIPMSPSPYNSWTQQ
K*RTDQNLLQNSQS*RIGGRMLSRVFGRGRVTLM
GW*GSGKISLLLWRTCFASSLTPATCYLQ*RAR
SASASTKPEV*SMS*RIKKFIFKGGELPVP*PW
KLEKSYCSQLT*KLKSLWVGESVNFRTAGKTWSP
SWQR*LSSSRAL*RPGTSVKRKSRS*KAGCKF*
RHKVKILFQSFTRTSITKKS*LRN*NSLWLAGL
RT*KNFKL*RRT*PGTFSWKM*WF*RSK*SICTD
NGRTSA*GWPYVNRRLKTDSIHGLYSMKKIKSC
VPGWCRWKTKFYRQRTLVLKK*LKSYRRTAWKK
*TCLVKTSYS*SRWVTS*SRPATNQEQLRSMTSS
TKLTIVGNIFLMSSDQG*RS*RRPLLLFSSWTK
T*ATFAPGWLELSLSFPSLLFMMSAMIKRSRRG
SLSSRIYSEILNNTAQGWSPCLTSVTSYCTTPMP
VQMRPSVTRSSRPPGAWTDAGGTFVPCPWSGA*
KSRRRGACGRSF*TTILALRTGSSQLRGRQPAQ
IPQRCCTRVPKRN*RGLRPFSGRFMSGSLSWSSS
TSSTGGWPGRTAQTRPAG*SRWSTRATSAGTTF
RGGSQPSCGDSGISPTRGKNLRAPGRAFWCGSQ
RWTCS*PTWSTSQRVTPMTRCAN*MASNRKLH*I
PTRLISSLCLGSS*FRRASPWMLC*LRMSWRNS
TATARRCLEGSPGSTGGSPPALRAWKMKRRPLR
MKQTWKTPEKSRLILGVNGERARNRHLLSPCVI*
WPQGTSGLAARPLSAWTPSPWSGTTQATWGAPP
LTKRTRRAHTTAHCQVNPFRMATRGMFPTALPV
PSITTSKWKVTGMFHLFPLRPAPLINHPMESYYY
LQARMVAKKARES*MATHSRKTGDWPVSQSSSQ
VPSTDGR*FKHRSFTISSK*NKICNS*TLISAP
SLLG*KKLKQSWKC*RWQSLPLISRK*N*E*RDC
RRY*KPLTLTRH*WSLST*AARNFCKPRAPNPQ
SSKVDSAS*ACSGKQHRAQWTAGEGAYDSRSCS
ARTSTS*VKICCCG*RVPRTGGRRLMSPIQRQTP
GLS*SVGGN*CNWKRSW*NVNLKWTCYRRFQTA
FSLRDMEKTVLKLKKRCMLLRRNSNSYGSKCPK
I*WPCREPRTQPHPCPASTR*TRGTSLLQHPCQL
PEQSSSEQ*ELQKARRRQRAGSPAAHGHSAPSS
QGWSGQPYPCSCSSCCCCSWPACCPPPKKTTAA
LRPTTLPGPFTPC*GTPMGHPPHRGHSWPQCYTT
CLIAKGAQHVAPDQSE*LSVGKVPGPVQTSSGL
TQHGLPGAQGSFQIVFLPRSREPVWQVPRVFWQ
N*LISLGISGNVSFLKSQALCEFWFVCKTALL*C
RYGQ*AVVSITYIIEASEDIPP*KWFRNS*APL
ADGNNQSYLIRLESVLLQSAGNFELPVIHQNVL
FST*LCSFILL*IFKEIVLHGCGYLCIFFNNAQS
V*IM*TSIFF*KN*ILAGAFD*CKVNAKLPT**
GCFCKLIF*DFFTSKVMLCFGF*LFGHGGGGGG
GLVQKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 1
PRSGARSAAPRAFGRT*KSERESEQKARSREPGKRGADLGRPRPAPLPLGRADVHFFN*DGHDIIS
IESKNGI*S*ASHRR*TGFLGHRRSPYFIAS*TG
RHPEESLHVLDKLTVGQAHFSLSYIRPIHRH*K
GACPPGSARSTFWATVASG*RI*YLPV*NQYRT
CLDIPKKPIN*ANKYSCY*YH*WKPIHYPWPNLD
NYPALSY*EACPDSFLQLQSAFPG*CECG*LIS
CLKSSS*EML*SASKMANVCKKGPSFVGSGTMR
HL*VCQCDRF*VKLEKWDGFFGHHSCLATRPN*H
EECEA*IQQRQSERGLQNCRTRIKNPQIAGTRR
CGCC*S**KVHHDLCGTVSAVFQRCPWDWRGGS
GKGERCYGLVNSAKGKTTEVAKGFRE*YLL*KV*
*PAVLYGVIQ*RKKVLFGCPVNKTGSG*AGQGS
FTVERSLGWPRSPD*CMENKAKLCLAPTPPSN*
SLAPGGRRAYG*RFVSLPGSLSSRDSDTRENDFI
QEPDG*I*ASFEHSPYL*K*G*KSLAIGTT*QI
GGNEKTNQQHFGEKIYSTSRISLLQVLSSWFGR
*SEIKIGYLEH*IWEQRICGIIAGRLA*IY*RKR
IPSST*YFFSKMWRNL*EFGWRMSEY**TVYDG
EI*CLYV*KKYI*CEVHSTKSAGMLGYLCGKPS
LTKGLL*GDKEGRN*RGTL*DTSPVESRTRYFK*
SRKFLSRSQQ*CGWIIYF*RTEKAE*KMEKVGF
KNST*NEPATDDKKTGSAHF*QFWKYSI*RRES
NC*VFNRYVSRTS*KL*SKYKGWRET*KRK*RIH
RATKSG*RC*KTHWTSGNLGGRSQICFGSR*CG
HLNGRIFEASYCQRLYV**AYGKK*RYVTNGYT
KYFKPGVLSTCSHNWASGKDSRS*RESPDQCGKT
HCSVEELN*RFTRFGHQAEDGRIPEGT*IIYDE
GSAVTGAKREPR*THFKTQGSTNNF*YKKSGQV
FESC*RTKK*CN*GHKDVFRRKE*RCLCQMGVSS
S*TVFICSTTKNRY*KRKA**QYFKT*KANK*R
KETYP*RKDQGSHQRT*GLLF*GRLPVPA*SPH
GSPEGAV*RAAFTEESTRSEETTQRL*TKDRKTS
EMCFRDSYDTAAHSGRHVEKRGDHHHI*E*RRG
SPQ*GTICKIR*SAIN*KGNGTHYEV*PGISVK
ASARRKHYGKGLQCIYK*FTREV*YIQRYS*TPP
AKQQIQDYF*FL**RGQE*FLSAG*TDRSTGHK
K*N*CSLERV*NYFIEVRKSCE*HKKAFCN*GK
RHTKGKRKRASDDS*YQNGIFRDSTAACVTCREG
ITSSLWLGPASP*NGHPGISSH*C*SHLSTPKE
YPRFHSKTD*NM*PLRRARQLCIKGVTPI*STR
NAEYYTEIQNTI*RNEPQGAEE*RYSQSSGRLFG
VSQNS*TLC*ARYRPFSLRYTGGTRKYVDSKK*
RGRNSSDERQGQTFG*MFEDARYEL*RC*TG**
HLL*KPA*CFFNKVI*DTWLWGTGGIH*GKQITR
GLYFQK**TP*KYSRCAESNQ*NWS*GSYCSSC
ETSEKIINQTG*GSR*I*RREETFTRNG*FSST
LQRWQRKNRESTVPKYSSLVGEYQSLGHRMS*TM
WESFGALKTISEF*KHLDNFDSKRRECHLPAGF
VHGKGEPEEKDSRD*NCQRRI**AFRSCRQDKP
GLQKSTILSK*NENF*RAPF*KRG*YYCG*MA*Y
K*EDRRLL*KSWSSSSFVGQTF*LKKCH**VDR
KGPSKNGITSID*RGQRKAEGRITSP*TKNFRI
F*KSG*NTVFAPKQ*NTS*IAGHGVLYFEQDGTC
TEVLNRRIQLPCTQWQHC*AKGGSRPSQDPDRD
D*IPLKSPVSF*QLGDLH*TRGDTTADSTAKTQ
YDIT*ESNRLSDS*TL*IEKAI*KCQ*FI*YQKK
CFARSLF*VIE*SMQEL**LVQQH*SEP*GVF*
IIRNKKECGTKATKTF*FLDS*RKKQ*NKAGGQ
RTEACEEASAQSTCEGAYQLARGSGIRIRKNGVH
MPGSSKGA*RLLAAATETPG*P*KPEEVVD*SR
REMERNGRTRGEN*AVLPSFS*KEGTV*ICGPI
EQLFEGIWVY*RRRNNNGSNMFDG*IPDITETTK
*NRGRG*VTTHRGPEL**SCT*CNSLDKRD*RV
PYGFEFIRRQNAT*GKNPKNQGNHFAEA*RGCQ
NRDHHEAG*EQRGPAGSEDPH*HQQPVGQHTPFS
*HLPKPSRKASTRRREIFTK*GGSEINAHRTKE
ETGSRLCSTTWSAGEESSVKDL*EIPQESPRFD
ILAKGVKISGKLPLGVH*KSQLQ*RALAGNKAST
RKSSSTTAGFCAKLGRSRSRT*FIPGLRHRPEY
YIGQFLQGICQFF**ACGSNSG*GKIAETAGTR
E*TQFTRWHIKEDFSFSKIRQAKYIFSGAEDY*R
*YKITSV*TKRFGKQACIC*AGDGMLSQQHSQI
KTLNRKERKVYSARQREAGHF*CAGVYSGISCS
GKVGGRLGNKQGFSCGNGYVKTTFS*CSRKHEKH
*R*AESQ*AAKSTFRIRYYVKK*TIRRDREIIY
PVGSKESSH*ATGTNRMS*QNRNWGLGSPQYRI
FGTAFGQFASGTYYFEEKQRKPILCPQRFSGIPC
CS*IFNESLVDRQGKS*SRTTGQCNVSGQN*KI
HSIHRKRERFFRQLENQMGEFIKPRD*HG*EIV
GKPD*AT*TWLGTSGTADSKEVFSAGSGI**IYN
PHE*GTGH*DFSATAAATSTVKAEVSRRTGREP
KHDCLDH*PPGYQAWIFCFKGAS*TSDEEDLGR
KRKEEFGGWNK*LEETMGNIGAITLRSRKSD*EV
*HKEQDERDYLMGQEFVG*T*SLHSPSPR*HSF
TDQKVQSDT*WHSS*AAVCGIVG*RGQR*GS*P
YNL*GQ*FK*YPRGLTESIPNAGFKINSKITAIR
I*VGRKKQFFCYNKEVSTYGSRK*NTDNSQGGD
SCHGS*TKTSPCYFGGISEGIARN*QWNLNTSS
GANKHL*GAECV*KIISGRSVEKS*D*DQQNTKI
HSEYM**SGTQDKVLQTIP*KNISASGGG*QYT
AQ*TIT*SRSK*RC*RGDL*S*RQTHRY*VLHL
TGIET*KSV*LYWTKLGFFTT*PITNPSI*KRKG
T*RKN*AVGHI*GRTWQISGIIK*NESY*FAN*
END*SSTKSS**LSGKQTAQCPNFKSEN*ESQV
FM**DNKEIK*K*DL**LIQGERNTTNKAECRRK
**VIQSSPKHGIRTLTKRIG*KELSGQTRNFLT
CFKSDKISITAAITYKFGN*TYSK*KGQL*SIS
GASLGRNV*Y*SCDCY*ETKRRKLF*SE*CGDKT
T*V*RSSDAA*HKH*FAHKCLE*CL*KSNTL*R
SSHQGSGEHHFPRSHHYTLQSRCW*SRRIFRDA
SSKTRGIGIHSSTHPGPH*ETGNDIQPRSQTTTS
VYFTGTSF*ELSNEGSFQSTGN*GRKVS*ELQM
L*KNGRGYLH*PQQNGDSSWTVHVLVATVLQRS
FRALGTEQGLGVKSYINQRRVNETTTDP*TLETQ
VHRK*WHMFAQDCVGSVGEMAEFAGSC*RVGDV
VRRTEAGMEICQ*RN*TRGNYFR*SSGRTP*NF
QNKRGSHHRGTL*AARLFMPIWRERGEATAVTDS
TSSAHQKYPECS*KLRGCGNCSSISRNYFYERT
MQQASSESSEK*RIGAD*NPRKTFLHKRDNCFE
EFLSTDHNFIPKYGIPGSPRKVRTI*GASKHP*E
RETNF*EYYGKTANQVFRNVHHSPCRD*IPGGR
MQKSFRRHR*ED*Q*SLKKLTIICNEEKNRRN*
QWAS*C*KDVAAEKQKY*ESSRNSKENVGRVRSM
AFQTK*AGF*SSGHC*TGPRTGSRMDG*LDDSF
PAVSASITESRV*NLTVE*GHS*DGGI**PSEE
H*GLDRKYQSFAGQSC*L*LFEDTESPC*HCADG
FGRFRTEAQSFTFNLYGSRRPVNNF*NR*INPI
HTRVK*SSNSFTTKNNGKPSTDSANG**CGCY*
I*SKINGKKSFKNQNYPIIKRNI*FFT*RTSQTW
GGHT*KYTSHEENHC*DSVLPSGTEVAPNRNET
SACVSADKSAFTRYKTIGKCDSRTK*VIKGSHK
TDQ*MG*RNRKFETDLK*LFSSVLP*TYVTRPS*
QAATTTGRNRTYGETDSEFEPEKRRPVGGLEGH
RTKPSPAFEARTRRSRKR*AASCNIRGRWSGRE
GCF*AEVEQKRLHVLPGSSRGRGGRKFREER*WR
*EGRAIASVLVFTLEA*QGHGRRQSFLILWNNC
SGSIWENKHL**FHGTNPHTRLTKH*ARPRMFP
KAQPNRRGHHTSY*G*HSGLF*RARRFGAQGGEN
*AGAHRSPACLQDPGGRAGAVAATSQRGS*AGN
IKRRHAAGAGTAAGRVPGYANRD*AQGCLSVRD
LQRSGPGR*WSHST*G*SAFPETENSEVQFRKSP
DDASGEAQ*RSASYHSKEILRARASRSSPTS*P
F*IGIHCN*KATIQQTKRFPAATGSGVKTNGTE
RFHQIHRI*C*ENVAPVLPT**RYNSGIICKQPG
IQP*K*RSRLDLVTPGPKWR*VAISAS*TLIKN
KAPTPSACGASGFHKYGYSTQRDYV*L*IPNN*
RTENLYHPT*RPAPRSK*PSDTGKYDRRSIYQFG
*KIV*TIPDPQSVPQQCGGDAGDAQTLQGGWFW
PAGALRDAGS*VEETLFSAK*QEG*SFESHDLA
WREHQLAP*MF*QPSSLPGAHSGCSCL*KQVPES
WPRLQPQLPE*NKEIISSAH*E*DIFTTVFE*N
QWAECC*TASESRCIYSGAGERREPSGQTKR*R
GEASFTLCFTPGGLQIRGCT*QYVGNAKSQVHRT
QQPFRH*EPARCFVARHGGTGEDWEGKACSWPL
KTNQK*SGVTGSNRKSQGFFPEACC*HVVDPSI
LCQNTSFFIAKQRDILGRTSNRS*NTRRKVTPMW
YEAAEFVAEMGRI**KLCIS*KGPGNSYIYIAL
CEFGGRNRGKISGKDFILPANKKKHWWKTRPAL
PNSERRQTVGGVCELS*IRGPDRKTGRAVVVPEQ
EN*P*APQAASSSQASAQL*QRFGSVNQVVGIF
PAYSELLERTVPQCVSGLGYNQKQHQQFF*VFK
RS**KILLEDCRYQYREPASSPERN*YSYTESFF
STV*TKMDNAHNSTSRYSRKTSPASNGEIAVS*
SNHRND*LDEQCGASNFR*RLRAFTKFCISS*T
SSSEAQGV*NGNGL*TVDS*LR*PVITSAKHL*C
RKQAL*KNGVCRAPGGDEPPVAPCTWNAE*KDT
TFRTTSRKYH*E*K*NTDLEQLAGSTRRETENF
TKT*KCDLSAEAAPGLSGYRKSTCN*IQSTR*VE
TKLSDFGEWGSAIVRRYSIPN**VISKEKQCSH
SGQSAQNLHAVSFTGVEDL*STL**SEYDDNPI
LVLHGTQQACGVIIGDLEMPGGEPSVSAR*S*EQ
*RELGETPGGYRQTQRSLPLCC*NNRREMPKYS
*KVDSGEPSHCRPVAEGPESAPALEGL*QCSW*
SCRKAEAAGSKVSTARKHQHVWKQPGRDPAPSPA
GHKGAAA*CAENKRSLSPKFQCPGSTPTTRRVQ
HPHAPPGPPALSPEGCLFGKDAACESK*I*VCS
LTV*GFWSPAGIFKRSYYA*RREFG*TSPTGKRK
S*LIPESCAGTDSPIT*Y*TFE*SEPQAPT**R
SCEDVTKYEPAMDSGHGHGTGALQ*ASGNWIE*
KVSLLL*KVDPTFGEDRRSTQSGCG*QPS*APGA
AENL*DVRS*SFYKPDNC*FLCHPVLTTPGHNR
NREQTRIYYRILKADGSVAECCPGCSAEEG*R*
WAGEAVARFHYFCGELVSLPH*HQPPAICSEGPG
ALQPLPNQKSDP*AEE*RNSFSKEANYLCPNLG
SWRKVTAHN*PEN*RVCG*ENQSTSGQLERHGA
PAGRDD*AVPEHCRDLGPV*KENQGVEKQAASFK
GTK*RSSSRASRGPP*RKRAD*GTRTVFG*LDS
ELERTSNYEGGLNPARSRGRCDGFEGANRAFAQ
TMGGPLLKGGHT*TGD*RQTQYMGCIQ*KK*RVV
CLAGADGKQSSTDSGH*Y*RND*KVTEGLHGRN
KLV**KQVTVKADG*PVDQGQQQIKSS*DR*QA
QQN*RSLATSF*CHRIKGEEAEGDLCFYSAVGQK
HEQPSHLVGSN*V*AFQACCL*CLR*SRDPEEA
R*AAGSTARY*TTQRRGGVRV*HL*RPTARLRC
LCK*DRV*LDPADHQEPGQTLEEHLCHVHGAAHE
NRGDVAPVAEVFRRLFSL*GLAQVS*EDGSLPK
FLRGVVHECQRGTEEV*GLSAADS*AAHSAGAH
QQAVPAAGPGEPHRHGQQAEADGPRGQPALGQPS
EAGHSRPAETQAFHQPEGRI*GHQGEHSGVAHR
DGPAADQRGALLRE*RR*QDAPTEWLPTGNYIK
YQQD*SAHCVWGAADSEERAPGCCAD*G*AGGTP
PLLPGGVWKGLPVPPAAHLLHSGLGR*KGGL*E
*NRHGRPQRNPD*FLA*TGRERGTVISSVPVSS
SGPRARAVWLRDPCQRGLHPPGVGPHRRRGGLLL
SRRGRGGPILQRTVR*IHFGWPLVACSRQPFLS
RASLQANGR*QECSTCSPCVQHPL*TTLWKATI
TSRHGWWQRRPASPEWQPTAGRRGTGRYHRAAVR
CLRQMGDDSSTGASQ*AQNKTKFATAEL*YQRH
HYLAEKN*SRAGNVKDGKASL*YPGNRTESEET
AGDTESL*HLQGISGLCQREQQGISANREPRIHR
APK*TPPAEPALGSSTGRSGQLERGLTTVAHAV
PGLPPVESKSAAVVSECQEPEAEGSCHRSKGRP
PGSPRVSEGTNATGKGAGRTSTSSGHVTGDFKQP
SH*GTWRRLY*S*RKGACY*EETQTVTGASVPR
FNGLAGNPEPSLTPAQLRRGRLGGPASCNIRAS
SPSKAVQSSENYRRRGGDREQGPRQHTATALLPL
KGGPGSPTPAAAPPAAAAPGLPAALLRRRLQLH
SGQQLCPVLLPHAEVHQWATPHIEGIAGHSATP
PA*LPRVPSTWPQTNLSDLVLARSRDLCRLLLGL
PSTGSLEPRAAFRLCSSPGAGNLCGRCPGYFGR
TS*LV*GSLEMSVS*RAKHFVNSGLFVKQHYYN
VGMVNEQWCPSHIL*KQARTFHPRNGSETHRHP*
LMETINHI*YA*NQFYSNQLAILSCRLYTKMFC
SVPSSALLYCFKFLKKLYCMDVVICAYFLTMPN
LYE*CKLRFFFKKIRF*LELLTNVK*MPNYRLDR
DVFVS*FSKTFSHPK*CFALGFNCLATAGVGAG
GWYRNLKLFVICTTQMFLIKKQN*PDFCCTE
Frame 2
QGAERAQLRPEPSAGPEKARGRASKRRDPESQASGAPTSGGPGPRPCPSAGRTFTSSTEMDMI*SP
LSQRMASSPELPTEDEQGSWGIDDLHISLQAEQ
EDTQKKAFTCWINSQLARHTSPSVISDLFTDIKK
GHVLLDLLEVLSGQQLPRDKGSNTFQCRINIEH
ALTFLRNRSIKLINIHVTDIIDGNPSIILGLIW
TIILHFHIEKLAQTLSCNYNQPSLDDVSVVDSSP
ASSPPAKKCSKVQARWQMSARKALLLWAQEQCA
TYESVNVTDFKSSWRNGMAFLAIIHALRPDLID
MKSVKHRSNKDNLREAFRIAEQELKIPRLLEPED
VDVVDPDEKSIMTYVAQFLQYSKDAPGTGEEAQ
GKVKDAMGWLTLQKEKLQKLLKDSENDTYFKKY
NSLLSFMESFNEEKKSFLDVLSIKRDLDELDKDH
LQLREAWDGLDHQINAWKIKLNYALPPPLHQTE
AWLQEVEELMDEDLSASQDHSQAVTLIQEKMTL
FKSLMDRFEHHSNILLTFENKDENHLPLVPPNKL
EEMKRRINNILEKKFILLLEFHYYKCLVLGLVD
EVKSKLDIWNIKYGSRESVELLLEDWHKFIEEK
EFLARLDTSFQKCGEIYKNLAGECQNINKQYMMV
KSDVCMYRKNIYNVKSTLQKVLACWATYVENLR
LLRACFEETKKEEIKEVPFETLAQWNLEHATLN
EAGNFLVEVSNDVVGSSISKELRRLNKRWRKLVS
KTQLEMNLPLMIKKQDQPTFDNSGNILSKEEKA
TVEFSTDMSVELPENYNQNIKAGEKHEKENEEF
TGQLKVAKDVEKLIGQVEIWEAEAKSVLDQDDVD
TSMEESLKHLIAKGSMFDELMARSEDMLQMDIQ
NISSQESFQHVLTTGLQAKIQEAKEKVQINVVK
LIAALKNLTDVSPDLDIRLKMEESQKELESYMMR
AQQLLGQRESPGELISKHKEALIISNTKSLAKY
LKAVEELKNNVTEDIKMSLEEKSRDVCAKWESL
HHELSLYVQQLKIDIEKGKLSDNILKLEKQINKE
KKLIRRGRTKGLIKEHEACFSEEGCLYQLNHHM
EVLRELCEELPSQKSQQEVKRLLKDYEQKIERL
LKCASEIHMTLQPTAGGTSKNEGTITTSENRGGD
PHSEAPFAKSDNQPSTEKAMEPTMKFSLASVLR
PLQEESIMEKDYSASINSLLERYDTYRDILEHH
LQNNKFRITSDFSSEEDRSSSCLQAKLTDLQVIK
NETDARWKEFEIISLKLENHVNDIKKPFVIKER
DTLKERERELQMTLNTRMESLETALRLVLPVEK
ASLLLCGSDLPLHKMAIQGFHLIDADRIYQHLRN
IQDSIAKQIEICNRLEEPGNFVLKELHPFDLHA
MQNIILKYKTQFEGMNHRVQRSEDTLKALEDFL
ASLRTAKLSAEPVTDLSASDTQVAQENTLTVKNK
EGEIHLMKDKAKHLDKCLKMLDMSFKDAERGDD
TSCENLLDAFSIKLSETHGYGVQEEFTEENKLL
EACIFKNNELLKNIQDVQSQISKIGLKDPTVPAV
KHRKKSLIRLDKVLDEYEEEKRHLQEMANSLPH
FKDGREKTVNQQCQNTVVLWENTKALVTECLEQ
CGRVLELLKQYQNFKSILTTLIQKEESVISLQAS
YMGKENLKKRIAEIEIVKEEFNEHLEVVDKINQ
VCKNLQFYLNKMKTFEEPPFEKEANIIVDRWLD
INEKTEDYYENLGRALALWDKLFNLKNVIDEWTE
KALQKMELHQLTEEDRERLKEELQVHEQKTSEF
SRRVAEIQFLLQSSEIPLELQVMESSILNKMEH
VQKCLTGESNCHALSGSTAELREDLDQAKTQIGM
TESLLKALSPSDSLEIFTKLEEIQQQILQQKHS
MILLENQIGCLTPELSELKKQYESVSDLFNTKK
SVLQDHFSKLLNDQCKNFNDWFSNIKVNLKECFE
SSETKKSVEQKLQKLSDFLTLEGRNSKIKQVDS
VLKHVKKHLPKAHVKELISWLVGQEFELEKMES
ICQARAKELEDSLQQLLRLQDDHRNLRKWLTNQE
EKWKGMEEPGEKTELFCQALARKREQFESVAQL
NNSLKEYGFTEEEEIIMEATCLMDRYQTLLRQL
SEIEEEDKLLPTEDQSFNDLAHDVIHWIKEIKES
LMVLNSSEGKMPLEERIQKIKEIILLKPEGDAR
IETIMKQAESSEAPLVQKTLTDISNQWDNTLHL
ASTYLSHQEKLLLEGEKYLQSKEDLRLMLIELKK
KQEAGFALQHGLQEKKAQLKIYKKFLKKAQDLT
SLLKELKSQGNYLLECTKNPSFSEEPWLEIKHL
HESLLQQLQDSVQNLDGHVREHDSYQVCVTDLNT
TLDNFSKEFVSFSDKPVDQIAVEEKLQKLQELE
NRLSLQDGTLKKILALAKSVKQNTSSVGQKIIK
DDIKSLQCKQKDLENRLASAKQEMECCLNSILKS
KRSTEKKGKFTLPGREKQATSDVQESTQESAAV
EKLEEDWEINKDSAVEMAMSKQLSLNAQESMKN
TEDERKVNELQNQPLELDTMLRNEQLEEIEKLYT
QLEAKKAAIKPLEQTECLNKTETGALVLHNIGY
SAQHLDNLLQALITLKKNKESQYCVLRDFQEYL
AAVESSMKALLTDKESLKVGPLDSVTYLDKIKKF
IASIEKEKDSLGNLKIKWENLSNHVTDMDKKLL
ESQIKQLEHGWEQVEQQIQKKYSQQVVEYDEFT
TLMNKVQDTEISLQQQQQHLQLRLKSPEERAGNQ
SMIALTTDLQATKHGFSVLKGQAELQMKRIWGE
KEKKNLEDGINNLKKQWETLEPLHLEAENQIKK
CDIRNKMKETILWAKNLLGELNPSIPLLPDDILS
QIRKCKVTHDGILARQQSVESLAEEVKDKVPSL
TTYEGSDLNNTLEDLRNQYQMLVLKSTQRSQQL
EFKLEERSNFFAIIRKFQLMVQESETLIIPRVET
AATEAELKHHHVTLEASQKELQEIDSGISTHLQ
ELTNIYEELNVFERLFLEDQLKNLKIRTNRIQR
FIQNTCNEVEHKIKFCRQFHEKTSALQEEADSIQ
RNELLLNQEVNKGVKEEIYNLKDRLTAIKCCIL
QVLKLKKVFDYIGLNWDFSQLDQLQTQVFEKEK
ELEEKIKQLDTFEEEHGKYQALLSKMRAIDLQIK
KMTEVVLKAPDSSPESRRLNAQILSQRIEKAKC
LCDEIIKKLNENKTFDDSFKEKEILQIKLNAEE
NDKLYKVLQNMVLELSPKELDEKNCQDKLETSLH
VLNQIKSQLQQPLLINLEIKHIQNEKDNCEAFQ
EQVWAEMCSIKAVTAIEKQREENSSEASDVETK
LREFEDLQMQLNTSIDLRTNVLNDAYENLTRYKE
AVTRAVESITSLEAIIIPYRVDVGNPEESLEMP
LRKQEELESTVAHIQDLTEKLGMISSPEAKLQL
QYTLQELVSKNSAMKEAFKAQETEAERYLENYKC
YRKMEEDIYTNLSKMETVLGQSMSSLPLSYREA
LERLEQSKALVSNLISTKEELMKLRQILRLLRL
RCTENDGICLLKIVSALWEKWLSLLEAAKEWEMW
CEELKQEWKFVSEEIEREAIILDNLQEELPEIS
KTKEAATTEELSELLDCLCQYGENVEKQQLLLT
LLLQRIRSIQNVPESSGAVETVPAFQEITSMKER
CNKLLQKVQKNKELVQTEIQERHSFTKEIIALK
NFFQQTTTSFQNMAFQDHPEKSEQFEELQSILK
KGKLTFENIMEKLRIKYSEMYTIVPAEIESQVEE
CRKALEDIDEKISNEVLKSSPSYAMRRKIEEIN
NGLHNVEKMLQQKSKNIEKAQEIQKKMWDELDL
WHSKLNELDSEVQDIVEQDPGQAQEWMDNLMIPF
QQYQQVSQRAECRTSQLNKATVKMEEYSDLLKS
TEAWIENTSHLLANPADYDSLRTLSHHASTVQM
ALEDSEQKHNLLHSIFMDLEDLSIIFETDELTQS
IQELSNQVTALQQKIMESLPQIQRMADDVVAIE
SEVKSMEKRVSKIKTILLSKEIFDFSPEEHLKH
GEVILENIRPMKKTIAEIVSYQVELRLPQTGMKP
LPVFQRTNQLLQDIKLLENVTQEQNELLKVVIK
QTNEWDEEIENLKQILNNYSAQFSLEHMSPDQA
DKLPQLQGEIERMEKQILSLNQRKEDLLVDLKAT
VLNLHQHLKQEQEGVERDRLPAVTSEEGGVAER
DASERKLNRRGSMSYLAAVEEEVEESSVKSDNG
DEKAEPSPQSWSSLWKHDKDMEEDRASSSSGTIV
QEAYGKISTSDNSMAQILTPDSLNTEQGPECSL
RPNQTEEGTTPPIEADTLDSSDAQGGLEPRVEK
TRPEPTEVLHACKTQVAELELWLQQANVAVEPET
LNADMQQVLEQQLVGCQAMLTEIEHKVAFLLET
CKDQGLGDNGATQHEAEALSLKLKTVKCNLEKV
QMMLQEKHSEDQHPTILKKSSEPEHQEALQPVNL
SELESIVTERPQFSRQKDFQQQQVLELKPMEQK
DFIKFIEFNAKKMWPQYCQHDNDTTQESSASNQ
ASSPENDVPDSILSPQGQNGDKWQYLHHELSSKI
KLPLPQLVEPQVSTNMGILPSVTMYNFRYPTTE
ELKTYTTQLEDLRQEASNLQTQENMTEEAYINL
DKKLFELFLTLSQCLSSVEEMLEMPRLYREDGSG
QQVHYETLALELKKLYLALSDKKGDLLKAMTWP
GENTNLLLECFDNLQVCLEHTQAAAVCRSKSLK
AGLDYNRSYQNEIKRLYHQLIKSKTSLQQSLNEI
SGQSVAEQLQKADAYTVELENAESRVAKLRDEG
ERLHLPYALLQEVYKLEDVLDSMWGMLRARYTE
LSSPFVTESQQDALLQGMVELVKIGKEKLAHGHL
KQTKSKVALQAQIENHKVFFQKLVADMLLIQAY
SAKILPSLLQNRETFWAEQVTEVKILEEKSRQC
GMKLQSLLQKWEEFDENYASLEKDLEILISTLPS
VSLVEETEERLVERISFYQQIKRNIGGKHARLY
QTLNEGKQLVASVSCPELEGQIAKLEEQWLSLN
KKIDHELHRLQALLKHLLSYNRDSDQLTKWLESS
QHTLNYWKEQSLNVSQDLDTIRSNINNFFEFSK
EVDEKSSLKTAVISIGNQLLHLKETDTATLRAS
LAQFEQKWTMLITQLPDIQEKLHQLQMEKLPSRK
AITEMISWMNNVEHQTSDEDSVHSPSSASQVKH
LLQKHKEFRMEMDYKQWIVDFVNQSLLQLSTCD
VESKRYERTEFAEHLGEMNRQWHRVHGMLNRKIQ
HLEQLLESITESENKIQILNNWLEAQEERLKTL
QKPESVISVQKLLLDCQDIENQLAIKSKALDEL
KQSYLTLESGAVPLLEDTASRIDELFQKRSSVLT
QVNQLKTSMQSVLQEWKIYDQLYDEVNMMTIRF
WYCMEHSKPVVLSLETLRCQVENLQSLQDEAES
SEGSWEKLQEVIGKLKGLCPSVAEIIEEKCQNTH
KRWTQVNQAIADQLQKAQSLLQLWKAYSNAHGE
AAARLKQQEAKFQQLANISMSGNNLAEILPPAL
QDIKELQHDVQKTKEAFLQNSSVLDRLPQPAESS
THMLLPGPLHSLQRAAYLEKMLLVKANEFEFVL
SQFKDFGVRLESLKGLIMHEEENLDRLHQQEKE
NPDSFLNHVLALTAQSPDIEHLNEVSLKLPLSDV
AVKTLQNMNRQWIRATATALERCSELQGIGLNE
KFLYCCEKWIQLLEKIEEALKVDVANSLPELLE
QQKTYKMLEAEVSINQTIADSYVTQSLQLLDTTE
IENRPEFITEFSKLTDRWQNAVQGVRQRKGDVD
GLVRQWQDFTTSVENLFRFLTDTSHLLSAVKGQ
ERFSLYQTRSLIHELKNKEIHFQRRRTTCALTLE
AGEKLLLTTDLKTKESVGRRISQLQDSWKDMEP
QLAEMIKQFQSTVETWDQCEKKIKELKSRLQVL
KAQSEDPLPELHEDLHNEKELIKELEQSLASWTQ
NLKELQTMKADLTRHVLVEDVMVLKEQIEHLHR
QWEDLCLRVAIRKQEIEDRLNTWVVFNEKNKEL
CAWLVQMENKVLQTADISIEEMIEKLQKDCMEEI
NLFSENKLQLKQMGDQLIKASNKSRAAEIDDKL
NKINDRWQHLFDVIGSRVKKLKETFAFIQQLDK
NMSNLRTWLARIESELSKPVVYDVCDDQEIQKRL
AEQQDLQRDIEQHSAGVESVFNICDVLLHDSDA
CANETECDSIQQTTRSLDRRWRNICAMSMERRM
KIEETWRLWQKFLDDYSRFEDWLKSAERTAACPN
SSEVLYTSAKEELKRFEAFQRQIHERLTQLELI
NKQYRRLARENRTDTASRLKQMVHEGNQRWDNL
QRRVTAVLRRLRHFTNQREEFEGTRESILVWLTE
MDLQLTNVEHFSESDADDKMRQLNGFQQEITLN
TNKIDQLIVFGEQLIQKSEPLDAVLIEDELEEL
HRYCQEVFGRVSRFHRRLTSCTPGLEDEKEASEN
ETDMEDPREIQTDSWRKRGESEEPSSPQSLCHL
VAPGHERSGCETPVSVDSIPLEWDHTGDVGGSS
SHEEDEEGPYYSALSGKSISDGHSWHVPDSPSCP
EHHYKQMEGDRNVPPVPPASSTPYKPPYGKLLL
PPGTDGGKEGPRVLNGNPQQEDGGLAGITEQQS
GAFDRWEMIQAQELHNKLKIKQNLQQLNSDISAI
TTWLKKTEAELEMLKMAKPPSDIQEIELRVKRL
QEILKAFDTYKALVVSVNVSSKEFLQTESPEST
ELQSRLRQLSLLWEAAQGAVDSWRGGLRQSLMQC
QDFHQLSQNLLLWLASAKNRRQKAHVTDPKADP
RALLECRRELMQLEKELVERQPQVDMLQEISNS
LLIKGHGEDCIEAEEKVHVIEKKLKQLREQVSQD
LMALQGTQNPASPLPSFDEVDSGDQPPATSVPA
PRAKQFRAVRTTEGEEETESRVPGSTRPQRSFL
SRVVRAALPLQLLLLLLLLLACLLPSSEEDYSCT
QANNFARSFYPMLRYTNGPPPT*RA*LATVLHH
LPDCQGCPARGPRPI*VT*CWQGPGTCADFFWA
YPARAPWSPGQLSDCVPPQEQGTCVAGAPGILAE
LVD*FRDLWKCQFPEEPSTL*ILVCL*NSIIIM
*VWSMSSGVHHIYYRSKRGHSTLEMVQKLIGTL
S*WKQSIIFNTLRISFTPISWQF*AAGYTPKCSV
QYLALLFYIALNF*RNCIAWMWLFVHIF*QCPI
CMNNVNFDFFLKKLDFSWSF*LM*SKCQTTDLI
GMFL*VNFLRLFHIQSDALLWVLTVWPRRGWGRG
VGTET*SCL*YVQLRCFSLKNKISQTSVVL
Sequence SYNE2.mAug05
GCAGAAGCCTATGAGTTGATTAATTCTAACAACTGGATACTGTGGTTTAGGGCTTGGAAGATGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCATCTCCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAG
CGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACACAGGCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGG
AGGGCCCATACTACAGCGCACTGTCAGATGTAGAAATCCCTGAAAATCCTGAGGCATATCTTAAAATGACCACAAAAACTTTGAAAGCGTCTTCTGGTAA
ATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCCCGAGCATCACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTT
CCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTACCTCCAGGCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCA
ACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTTCGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAA
GCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCATCACTACTTGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATG
GCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGATACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTG
TCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCAAAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACA
GGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTGCCAGGGCTCTAAGACACGGCCGCGCTCTGATGTGTTGTTTTTTAAGGAC
TTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCCCGGGCTC
TCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCAT
TAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGATTTAATG
GCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGAG
CAAAGCAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCTCAAGGGT
GGTCCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGCC
AACAACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCACCTGCCTG
ATTGCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTTACCCAGC
ACGGGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGAACTAGTT
GATTAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATGTAGGTAT
GGTCAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTAGCTGATG
GAAACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGTTCAGTAC
CTAGCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATCTGTATGA
ATAATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAGGGATGTT
TTTGTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGGGGTTGGT
ACAGAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
AEAYELINSNNWILWFRAWKMKRRPLRMKQTWKTPEKSRLILGVNGERARNRHLLSPCVI*WPQGTS
GLAARPLSAWTPSPWSGTTQATWGAPPLTKRTR
RAHTTAHCQM*KSLKILRHILK*PQKL*KRLLV
NPFRMATRGMFPTALPVPSITTSKWKVTGMFHLF
PLRPAPLINHPMESYYYLQARMVAKKARES*MA
THSRKTGDWPVSQSSSQVPSTDGR*FKHRSFTI
SSK*NKICNS*TLISAPSLLG*KKLKQSWKC*RW
QSLPLISRK*N*E*RDCRRY*KPLTLTRH*WSL
ST*AARNFCKPRAPNPQSSKVDSAS*ACSGKQH
RAQWTAGEGAYDSRSCSARALRHGRALMCCFLRT
STS*VKICCCG*RVPRTGGRRLMSPIQRQTPGL
S*SVGGN*CNWKRSW*NVNLKWTCYRRFQTAFS
LRDMEKTVLKLKKRCMLLRRNSNSYGSKCPKI*W
PCREPRTQPHPCPASTR*TRGTSLLQHPCQLPE
QSSSEQ*ELQKARRRQRAGSPAAHGHSAPSSQG
WSGQPYPCSCSSCCCCSWPACCPPPKKTTAALRP
TTLPGPFTPC*GTPMGHPPHRGHSWPQCYTTCL
IAKGAQHVAPDQSE*LSVGKVPGPVQTSSGLTQ
HGLPGAQGSFQIVFLPRSREPVWQVPRVFWQN*L
ISLGISGNVSFLKSQALCEFWFVCKTALL*CRY
GQ*AVVSITYIIEASEDIPP*KWFRNS*APLAD
GNNQSYLIRLESVLLQSAGNFELPVIHQNVLFST
*LCSFILL*IFKEIVLHGCGYLCIFFNNAQSV*
IM*TSIFF*KN*ILAGAFD*CKVNAKLPT**GC
FCKLIF*DFFTSKVMLCFGF*LFGHGGGGGGGLV
QKLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 1
QKPMS*LILTTGYCGLGLGR*KGGL*E*NRHGRPQRNPD*FLA*TGRERGTVISSVPVSSSGPRAR
AVWLRDPCQRGLHPPGVGPHRRRGGLLLSRRGRG
GPILQRTVRCRNP*KS*GIS*NDHKNFESVFW*
IHFGWPLVACSRQPFLSRASLQANGR*QECSTC
SPCVQHPL*TTLWKATITSRHGWWQRRPASPEWQ
PTAGRRGTGRYHRAAVRCLRQMGDDSSTGASQ*
AQNKTKFATAEL*YQRHHYLAEKN*SRAGNVKD
GKASL*YPGNRTESEETAGDTESL*HLQGISGLC
QREQQGISANREPRIHRAPK*TPPAEPALGSST
GRSGQLERGLTTVAHAVPGL*DTAAL*CVVF*G
LPPVESKSAAVVSECQEPEAEGSCHRSKGRPPGS
PRVSEGTNATGKGAGRTSTSSGHVTGDFKQPSH
*GTWRRLY*S*RKGACY*EETQTVTGASVPRFN
GLAGNPEPSLTPAQLRRGRLGGPASCNIRASSPS
KAVQSSENYRRRGGDREQGPRQHTATALLPLKG
GPGSPTPAAAPPAAAAPGLPAALLRRRLQLHSG
QQLCPVLLPHAEVHQWATPHIEGIAGHSATPPA*
LPRVPSTWPQTNLSDLVLARSRDLCRLLLGLPS
TGSLEPRAAFRLCSSPGAGNLCGRCPGYFGRTS
*LV*GSLEMSVS*RAKHFVNSGLFVKQHYYNVGM
VNEQWCPSHIL*KQARTFHPRNGSETHRHP*LM
ETINHI*YA*NQFYSNQLAILSCRLYTKMFCSV
PSSALLYCFKFLKKLYCMDVVICAYFLTMPNLYE
*CKLRFFFKKIRF*LELLTNVK*MPNYRLDRDV
FVS*FSKTFSHPK*CFALGFNCLATAGVGAGGW
YRNLKLFVICTTQMFLIKKQN*PDFCCTE
Frame 2
RSL*VD*F*QLDTVV*GLEDEKEASENETDMEDPREIQTDSWRKRGESEEPSSPQSLCHLVAPGHE
RSGCETPVSVDSIPLEWDHTGDVGGSSSHEEDE
EGPYYSALSDVEIPENPEAYLKMTTKTLKASSGK
SISDGHSWHVPDSPSCPEHHYKQMEGDRNVPPV
PPASSTPYKPPYGKLLLPPGTDGGKEGPRVLNG
NPQQEDGGLAGITEQQSGAFDRWEMIQAQELHNK
LKIKQNLQQLNSDISAITTWLKKTEAELEMLKM
AKPPSDIQEIELRVKRLQEILKAFDTYKALVVS
VNVSSKEFLQTESPESTELQSRLRQLSLLWEAAQ
GAVDSWRGGLRQSLMQCQGSKTRPRSDVLFFKD
FHQLSQNLLLWLASAKNRRQKAHVTDPKADPRA
LLECRRELMQLEKELVERQPQVDMLQEISNSLLI
KGHGEDCIEAEEKVHVIEKKLKQLREQVSQDLM
ALQGTQNPASPLPSFDEVDSGDQPPATSVPAPR
AKQFRAVRTTEGEEETESRVPGSTRPQRSFLSRV
VRAALPLQLLLLLLLLLACLLPSSEEDYSCTQA
NNFARSFYPMLRYTNGPPPT*RA*LATVLHHLP
DCQGCPARGPRPI*VT*CWQGPGTCADFFWAYPA
RAPWSPGQLSDCVPPQEQGTCVAGAPGILAELV
D*FRDLWKCQFPEEPSTL*ILVCL*NSIIIM*V
WSMSSGVHHIYYRSKRGHSTLEMVQKLIGTLS*W
KQSIIFNTLRISFTPISWQF*AAGYTPKCSVQY
LALLFYIALNF*RNCIAWMWLFVHIF*QCPICM
NNVNFDFFLKKLDFSWSF*LM*SKCQTTDLIGMF
L*VNFLRLFHIQSDALLWVLTVWPRRGWGRGVG
TET*SCL*YVQLRCFSLKNKISQTSVVL
Sequence SYNE2.fAug05
CCAGAGTCTAATCAAAGGATTAGCCTACAGGTTTGGAAACAACAGCCGCTTGAGTTGAAGGCTTTTTACCCCCTTCTTGTGATTAAATGCAGGAACTAGAACAGTCTTTGGCTAGCTGGACTCAGAACTTGAAAGAACTTCAAACTATGAAGGCGGACTTAACCCGGCACGTTCTCGTGGAAGATGTGATGGTTTTGAAG
GAGCAAATAGAGCATTTGCACAGACAATGGGAGGACCTCTGCTTAAGGGTGGCCATACGTAAACAGGAGATTGAAGACAGACTCAATACATGGGTTGTAT
TCAATGAAAAAAATAAAGAGTTGTGTGCCTGGCTGGTGCAGATGGAAAACAAAGTTCTACAGACAGCGGACATTAGTATTGAAGAAATGATTGAAAAGTT
ACAGAAGGACTGCATGGAAGAAATAAACTTGTTTAGTGAAAACAAGTTACAGTTAAAGCAGATGGGTGACCAGTTGATCAAGGCCAGCAACAAATCAAGA
GCAGCTGAGATCGATGACAAGCTCAACAAAATTAACGATCGTTGGCAACATCTTTTTGATGTCATCGGATCAAGGGTGAAGAAGCTGAAGGAGACCTTTG
CTTTTATTCAGCAGTTGGACAAAAACATGAGCAACCTTCGCACCTGGTTGGCTCGAATTGAGTCTGAGCTTTCCAAGCCTGTTGTTTATGATGTCTGCGA
TGATCAAGAGATCCAGAAGAGGCTCGCTGAGCAGCAGGATCTACAGCGAGATATTGAACAACACAGCGCAGGGGTGGAGTCCGTGTTTAACATCTGTGAC
GTCCTACTGCACGACTCCGATGCCTGTGCAAATGAGACCGAGTGTGACTCGATCCAGCAGACCACCAGGAGCCTGGACAGACGCTGGAGGAACATTTGTG
CCATGTCCATGGAGCGGCGCATGAAAATCGAGGAGACGTGGCGCCTGTGGCAGAAGTTTTTAGACGACTATTCTCGCTTTGAGGACTGGCTCAAGTCAGC
TGAGAGGACGGCAGCCTGCCCAAATTCCTCAGAGGTGTTGTACACGAGTGCCAAAGAGGAACTGAAGAGGTTTGAGGCCTTTCAGCGGCAGATTCATGAG
CGGCTCACTCAGCTGGAGCTCATCAACAAGCAGTACCGGCGGCTGGCCCGGGAGAACCGCACAGACACGGCCAGCAGGCTGAAGCAGATGGTCCACGAGG
GCAACCAGCGCTGGGACAACCTTCAGAGGCGGGTCACAGCCGTCCTGCGGAGACTCAGGCATTTCACCAACCAGAGGGAAGAATTTGAGGGCACCAGGGA
GAGCATTCTGGTGTGGCTCACAGAGATGGACCTGCAGCTGACCAACGTGGAGCACTTCTCAGAGAGTGACGCCGATGACAAGATGCGCCAACTGAATGGC
TTCCAACAGGAAATTACATTAAATACCAACAAGATTGATCAGCTCATTGTGTTTGGGGAGCAGCTGATTCAGAAGAGCGAGCCCCTGGATGCTGTGCTGA
TTGAGGATGAGCTGGAGGAACTCCACCGCTACTGCCAGGAGGTGTTTGGAAGGGTCTCCCGGTTCCACCGGCGGCTCACCTCCTGCACTCCGGGCTTGGA
AGATGAAAAGGAGGCCTCTGAGAATGAAACAGACATGGAAGACCCCAGAGAAATCCAGACTGATTCTTGGCGTAAACGGGGAGAGAGCGAGGAACCGTCA
TCTCCTCAGTCCCTGTGTCATCTAGTGGCCCCAGGGCACGAGCGGTCTGGCTGCGAGACCCCTGTCAGCGTGGACTCCATCCCCCTGGAGTGGGACCACA
CAGGCGACGTGGGGGGCTCCTCCTCTCACGAAGAGGACGAGGAGGGCCCATACTACAGCGCACTGTCAGATGTAGAAATCCCTGAAAATCCTGAGGCATA
TCTTAAAATGACCACAAAAACTTTGAAAGCGTCTTCTGGTAAATCCATTTCGGATGGCCACTCGTGGCATGTTCCCGACAGCCCTTCCTGTCCCGAGCAT
CACTACAAGCAAATGGAAGGTGACAGGAATGTTCCACCTGTTCCCCCTGCGTCCAGCACCCCTTATAAACCACCCTATGGAAAGCTACTATTACCTCCAG
GCACGGATGGTGGCAAAGAAGGCCCGCGAGTCCTGAATGGCAACCCACAGCAGGAAGACGGGGGACTGGCCGGTATCACAGAGCAGCAGTCAGGTGCCTT
CGACAGATGGGAGATGATTCAAGCACAGGAGCTTCACAATAAGCTCAAAATAAAACAAAATTTGCAACAGCTGAACTCTGATATCAGCGCCATCACTACT
TGGCTGAAAAAAACTGAAGCAGAGCTGGAAATGTTAAAGATGGCAAAGCCTCCCTCTGATATCCAGGAAATAGAACTGAGAGTGAAGAGACTGCAGGAGA
TACTGAAAGCCTTTGACACTTACAAGGCATTAGTGGTCTCTGTCAACGTGAGCAGCAAGGAATTTCTGCAAACCGAGAGCCCCGAATCCACAGAGCTCCA
AAGTAGACTCCGCCAGCTGAGCCTGCTCTGGGAAGCAGCACAGGGCGCAGTGGACAGCTGGAGAGGGGGCTTACGACAGTCGCTCATGCAGTGCCAGGAC
TTCCACCAGTTGAGTCAAAATCTGCTGCTGTGGTTAGCGAGTGCCAAGAACCGGAGGCAGAAGGCTCATGTCACCGATCCAAAGGCAGACCCCCGGGCTC
TCCTAGAGTGTCGGAGGGAACTAATGCAACTGGAAAAGGAGCTGGTAGAACGTCAACCTCAAGTGGACATGTTACAGGAGATTTCAAACAGCCTTCTCAT
TAAGGGACATGGAGAAGACTGTATTGAAGCTGAAGAAAAGGTGCATGTTATTGAGAAGAAACTCAAACAGTTACGGGAGCAAGTGTCCCAAGATTTAATG
GCCTTGCAGGGAACCCAGAACCCAGCCTCACCCCTGCCCAGCTTCGACGAGGTAGACTCGGGGGACCAGCCTCCTGCAACATCCGTGCCAGCTCCCCGAG
CAAAGTTCAGAGCAGTGAGAACTACAGAAGGCGAGGAGGAGACAGAGAGCAGGGTCCCCGGCAGCACACGGCCACAGCGCTCCTTCCTCTCAAGGGTGGT
CCGGGCAGCCCTACCCCTGCAGCTGCTCCTCCTGCTGCTGCTGCTCCTGGCCTGCCTGCTGCCCTCCTCCGAAGAAGACTACAGCTGCACTCAGGCCAAC
AACTTTGCCCGGTCCTTTTACCCCATGCTGAGGTACACCAATGGGCCACCCCCCACATAGAGGGCATAGCTGGCCACAGTGCTACACCACCTGCCTGATT
GCCAAGGGTGCCCAGCACGTGGCCCCAGACCAATCTGAGTGACTTAGTGTTGGCAAGGTCCCGGGACCTGTGCAGACTTCTTCTGGGCTTACCCAGCACG
GGCTCCCTGGAGCCCAGGGCAGCTTTCAGATTGTGTTCCTCCCCAGGAGCAGGGAACCTGTGTGGCAGGTGCCCCGGGTATTTTGGCAGAACTAGTTGAT
TAGTTTAGGGATCTCTGGAAATGTCAGTTTCCTGAAGAGCCAAGCACTTTGTGAATTCTGGTTTGTTTGTAAAACAGCATTATTATAATGTAGGTATGGT
CAATGAGCAGTGGTGTCCATCACATATATTATAGAAGCAAGCGAGGACATTCCACCCTAGAAATGGTTCAGAAACTCATAGGCACCCTTAGCTGATGGAA
ACAATCAATCATATTTAATACGCTTAGAATCAGTTTTACTCCAATCAGCTGGCAATTTTGAGCTGCCGGTTATACACCAAAATGTTCTGTTCAGTACCTA
GCTCTGCTCTTTTATATTGCTTTAAATTTTTAAAGAAATTGTATTGCATGGATGTGGTTATTTGTGCATATTTTTTAACAATGCCCAATCTGTATGAATA
ATGTAAACTTCGATTTTTTTTTAAAAAAATTAGATTTTAGCTGGAGCTTTTGACTAATGTAAAGTAAATGCCAAACTACCGACTTGATAGGGATGTTTTT
GTAAGTTAATTTTCTAAGACTTTTTCACATCCAAAGTGATGCTTTGCTTTGGGTTTTAACTGTTTGGCCACGGCGGGGGTGGGGGCGGGGGGTTGGTACA
GAAACTTGAAGCTGTTTGTGATATGTACAACTCAGATGTTTCTCATTAAAAAACAAAATTAGCCAGACTTCTGTTGTACTGAA
- SYNE2.u.f.128 (forward primer)
- SYNE2.u.r.125 (reverse primer)
- Single exon skipping
Frame 0
PESNQRISLQVWKQQPLELKAFYPLLVIKCRN*NSLWLAGLRT*KNFKL*RRT*PGTFSWKM*WF*R
SK*SICTDNGRTSA*GWPYVNRRLKTDSIHGLY
SMKKIKSCVPGWCRWKTKFYRQRTLVLKK*LKS
YRRTAWKK*TCLVKTSYS*SRWVTS*SRPATNQE
QLRSMTSSTKLTIVGNIFLMSSDQG*RS*RRPL
LLFSSWTKT*ATFAPGWLELSLSFPSLLFMMSA
MIKRSRRGSLSSRIYSEILNNTAQGWSPCLTSVT
SYCTTPMPVQMRPSVTRSSRPPGAWTDAGGTFV
PCPWSGA*KSRRRGACGRSF*TTILALRTGSSQ
LRGRQPAQIPQRCCTRVPKRN*RGLRPFSGRFMS
GSLSWSSSTSSTGGWPGRTAQTRPAG*SRWSTR
ATSAGTTFRGGSQPSCGDSGISPTRGKNLRAPG
RAFWCGSQRWTCS*PTWSTSQRVTPMTRCAN*MA
SNRKLH*IPTRLISSLCLGSS*FRRASPWMLC*
LRMSWRNSTATARRCLEGSPGSTGGSPPALRAW
KMKRRPLRMKQTWKTPEKSRLILGVNGERARNRH
LLSPCVI*WPQGTSGLAARPLSAWTPSPWSGTT
QATWGAPPLTKRTRRAHTTAHCQM*KSLKILRH
ILK*PQKL*KRLLVNPFRMATRGMFPTALPVPSI
TTSKWKVTGMFHLFPLRPAPLINHPMESYYYLQ
ARMVAKKARES*MATHSRKTGDWPVSQSSSQVP
STDGR*FKHRSFTISSK*NKICNS*TLISAPSLL
G*KKLKQSWKC*RWQSLPLISRK*N*E*RDCRR
Y*KPLTLTRH*WSLST*AARNFCKPRAPNPQSS
KVDSAS*ACSGKQHRAQWTAGEGAYDSRSCSART
STS*VKICCCG*RVPRTGGRRLMSPIQRQTPGL
S*SVGGN*CNWKRSW*NVNLKWTCYRRFQTAFS
LRDMEKTVLKLKKRCMLLRRNSNSYGSKCPKI*W
PCREPRTQPHPCPASTR*TRGTSLLQHPCQLPE
QSSEQ*ELQKARRRQRAGSPAAHGHSAPSSQGW
SGQPYPCSCSSCCCCSWPACCPPPKKTTAALRPT
TLPGPFTPC*GTPMGHPPHRGHSWPQCYTTCLI
AKGAQHVAPDQSE*LSVGKVPGPVQTSSGLTQH
GLPGAQGSFQIVFLPRSREPVWQVPRVFWQN*LI
SLGISGNVSFLKSQALCEFWFVCKTALL*CRYG
Q*AVVSITYIIEASEDIPP*KWFRNS*APLADG
NNQSYLIRLESVLLQSAGNFELPVIHQNVLFST*
LCSFILL*IFKEIVLHGCGYLCIFFNNAQSV*I
M*TSIFF*KN*ILAGAFD*CKVNAKLPT**GCF
CKLIF*DFFTSKVMLCFGF*LFGHGGGGGGGLVQ
KLEAVCDMYNSDVSH*KTKLARLLLY*
Frame 1
QSLIKGLAYRFGNNSRLS*RLFTPFL*LNAGTRTVFG*LDSELERTSNYEGGLNPARSRGRCDGFE
GANRAFAQTMGGPLLKGGHT*TGD*RQTQYMGCI
Q*KK*RVVCLAGADGKQSSTDSGH*Y*RND*KV
TEGLHGRNKLV**KQVTVKADG*PVDQGQQQIK
SS*DR*QAQQN*RSLATSF*CHRIKGEEAEGDLC
FYSAVGQKHEQPSHLVGSN*V*AFQACCL*CLR
*SRDPEEAR*AAGSTARY*TTQRRGGVRV*HL*
RPTARLRCLCK*DRV*LDPADHQEPGQTLEEHLC
HVHGAAHENRGDVAPVAEVFRRLFSL*GLAQVS
*EDGSLPKFLRGVVHECQRGTEEV*GLSAADS*
AAHSAGAHQQAVPAAGPGEPHRHGQQAEADGPRG
QPALGQPSEAGHSRPAETQAFHQPEGRI*GHQG
EHSGVAHRDGPAADQRGALLRE*RR*QDAPTEW
LPTGNYIKYQQD*SAHCVWGAADSEERAPGCCAD
*G*AGGTPPLLPGGVWKGLPVPPAAHLLHSGLG
R*KGGL*E*NRHGRPQRNPD*FLA*TGRERGTV
ISSVPVSSSGPRARAVWLRDPCQRGLHPPGVGPH
RRRGGLLLSRRGRGGPILQRTVRCRNP*KS*GI
S*NDHKNFESVFW*IHFGWPLVACSRQPFLSRA
SLQANGR*QECSTCSPCVQHPL*TTLWKATITSR
HGWWQRRPASPEWQPTAGRRGTGRYHRAAVRCL
RQMGDDSSTGASQ*AQNKTKFATAEL*YQRHHY
LAEKN*SRAGNVKDGKASL*YPGNRTESEETAGD
TESL*HLQGISGLCQREQQGISANREPRIHRAP
K*TPPAEPALGSSTGRSGQLERGLTTVAHAVPG
LPPVESKSAAVVSECQEPEAEGSCHRSKGRPPGS
PRVSEGTNATGKGAGRTSTSSGHVTGDFKQPSH
*GTWRRLY*S*RKGACY*EETQTVTGASVPRFN
GLAGNPEPSLTPAQLRRGRLGGPASCNIRASSPS
KVQSSENYRRRGGDREQGPRQHTATALLPLKGG
PGSPTPAAAPPAAAAPGLPAALLRRRLQLHSGQ
QLCPVLLPHAEVHQWATPHIEGIAGHSATPPA*L
PRVPSTWPQTNLSDLVLARSRDLCRLLLGLPST
GSLEPRAAFRLCSSPGAGNLCGRCPGYFGRTS*
LV*GSLEMSVS*RAKHFVNSGLFVKQHYYNVGMV
NEQWCPSHIL*KQARTFHPRNGSETHRHP*LME
TINHI*YA*NQFYSNQLAILSCRLYTKMFCSVP
SSALLYCFKFLKKLYCMDVVICAYFLTMPNLYE*
CKLRFFFKKIRF*LELLTNVK*MPNYRLDRDVF
VS*FSKTFSHPK*CFALGFNCLATAGVGAGGWY
RNLKLFVICTTQMFLIKKQN*PDFCCTE
Frame 2
RV*SKD*PTGLETTAA*VEGFLPPSCD*MQELEQSLASWTQNLKELQTMKADLTRHVLVEDVMVLK
EQIEHLHRQWEDLCLRVAIRKQEIEDRLNTWVV
FNEKNKELCAWLVQMENKVLQTADISIEEMIEKL
QKDCMEEINLFSENKLQLKQMGDQLIKASNKSR
AAEIDDKLNKINDRWQHLFDVIGSRVKKLKETF
AFIQQLDKNMSNLRTWLARIESELSKPVVYDVCD
DQEIQKRLAEQQDLQRDIEQHSAGVESVFNICD
VLLHDSDACANETECDSIQQTTRSLDRRWRNIC
AMSMERRMKIEETWRLWQKFLDDYSRFEDWLKSA
ERTAACPNSSEVLYTSAKEELKRFEAFQRQIHE
RLTQLELINKQYRRLARENRTDTASRLKQMVHE
GNQRWDNLQRRVTAVLRRLRHFTNQREEFEGTRE
SILVWLTEMDLQLTNVEHFSESDADDKMRQLNG
FQQEITLNTNKIDQLIVFGEQLIQKSEPLDAVL
IEDELEELHRYCQEVFGRVSRFHRRLTSCTPGLE
DEKEASENETDMEDPREIQTDSWRKRGESEEPS
SPQSLCHLVAPGHERSGCETPVSVDSIPLEWDH
TGDVGGSSSHEEDEEGPYYSALSDVEIPENPEAY
LKMTTKTLKASSGKSISDGHSWHVPDSPSCPEH
HYKQMEGDRNVPPVPPASSTPYKPPYGKLLLPP
GTDGGKEGPRVLNGNPQQEDGGLAGITEQQSGAF
DRWEMIQAQELHNKLKIKQNLQQLNSDISAITT
WLKKTEAELEMLKMAKPPSDIQEIELRVKRLQE
ILKAFDTYKALVVSVNVSSKEFLQTESPESTELQ
SRLRQLSLLWEAAQGAVDSWRGGLRQSLMQCQD
FHQLSQNLLLWLASAKNRRQKAHVTDPKADPRA
LLECRRELMQLEKELVERQPQVDMLQEISNSLLI
KGHGEDCIEAEEKVHVIEKKLKQLREQVSQDLM
ALQGTQNPASPLPSFDEVDSGDQPPATSVPAPR
AKFRAVRTTEGEEETESRVPGSTRPQRSFLSRVV
RAALPLQLLLLLLLLLACLLPSSEEDYSCTQAN
NFARSFYPMLRYTNGPPPT*RA*LATVLHHLPD
CQGCPARGPRPI*VT*CWQGPGTCADFFWAYPAR
APWSPGQLSDCVPPQEQGTCVAGAPGILAELVD
*FRDLWKCQFPEEPSTL*ILVCL*NSIIIM*VW
SMSSGVHHIYYRSKRGHSTLEMVQKLIGTLS*WK
QSIIFNTLRISFTPISWQF*AAGYTPKCSVQYL
ALLFYIALNF*RNCIAWMWLFVHIF*QCPICMN
NVNFDFFLKKLDFSWSF*LM*SKCQTTDLIGMFL
*VNFLRLFHIQSDALLWVLTVWPRRGWGRGVGT
ET*SCL*YVQLRCFSLKNKISQTSVVL
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