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Sequences

Sequence FANCA.bAug05

TCACGAGATACTTTGTGCAGCTGCTTATCTCCAGGCCTTATTAAAAAGTTTCAGTTCCTCATGTTCAGATTGTTCTCAGAGGCCCGACAGCCTCTTTCTG
AGGAGGACGTAGCCAGCCTTTCCTGGAGACCCTTGCACCTTCCTTCTGCAGACTGGCAGAGAGCTGCCCTCTCTCTCTGGACACACAGAACCTTCCGAGA
GGTGTTGAAAGAGGAAGATGTTCACTTAACTTACCAAGACTGGTTACACCTGGAGCTGGAAATTCAACCTGAAGCTGATGCTCTTTCAGATACTGAACGC
TCAAGGAGTTATGACCACTCAGAAAATTCTGATTTGGTCTTTGGTGGCCGCACAGGAAATGAGGATATTATTTCCAGATTGCAGGAGATGGTAGCTGACC
TGGAGCTGCAGCAAGACCTCATAGTGCCTCTCGGCCACACCCCTTCCCAGGAGCACTTCCTCTTTGAGATTTTCCGCAGACGGCTCCAGGCTCTGACAAG
CGGGTGGAGCGTGGCTGCCAGCCTTCAGAGACAGAGGGAGCTGCTAATGTACAAACGGATCCTCCTCCGCCTGCCTTCGTCTGTCCTCTGCGGCAGCAGC
TTCCAGGCAGAACAGCCCATCACTGCCAGATGCGAGCAGTTCTTCCACTTGGTCAACTCTGAGATGAGAAACTTCTGCTCCCACGGAGGTGCCCTGACAC
AGGACATCACTGCCCACTTCTTCAGGGGCCTCCTGAACGCCTGTCTGCGGAGCAGAGACCCCTCCCTGATGGTCGACTTCATACTGGCCAAGTGCCAGAC
GAAATGCCCCTTAATTTTGACCTCTGCTCTGGTGTGGTGGCCGAGCCTGGAGCCTGTGCTGCTCTGCCGGTGGAGGAGACACTGCCAGAGCCCGCTGCCC
CGGGAACTGCAGAAGCTACAAGAAGGCCGGCAGTTTGCCAGCGATTTCCTCTCCCCTGAGGCTGCCTCCCCAGCACCCAACCCGGACTGGCTCTCAGCTG
CTGCACTGCACTTTGCGATTCAACAAGTCAGGGAAGAAAACATCAGGAAGCAGCTAAAGAAGCTGGACTGCGAGAGAGAGGAGCTATTGGTTTTCCTTTT
CTTCTTCTCCTTGATGGGCCTGCTGTCGTCACATCTGACCTCAAATAGCACCACAGACCTGCCAAAGGCTTTCCACGTTTGTGCAGCAATCCTCGAGTGT
TTAGAGAAGAGGAAGATATCCTGGCTGGCACTCTTTCAGTTGACAGAGAGTGACCTCAGGCTGGGGCGGCTCCTCCTCCGTGTGGCCCCGGATCAGCACA
CCAGGCTGCTGCCTTTCGCTTTTTACAGTCTTCTCTCCTACTTCCATGAAGACGCGGCCATCAGGGAAGAGGCCTTCCTGCATGTTGCTGTGGACATGTA
CTTGAAGCTGGTCCAGCTCTTCGTGGCTGGGGATACAAGCACAGTTTCACCTCCAGCTGGCAGGAGCCTGGAGCTCAAGGGTCAGGCAGGGCAACCCCGT
GGAACTGATAACAAAAGCTCGTCTTTTTCTGCTGCAGTTAATACCTCGGTGCCCGAAAAAGAGCTTCTCACACGTGGCAGAGCTGCTGGCTGATCGTGGG
GACTGCGACCCAGAGGTGAGCGCCGCCCTCCAGAGCAGACAGCAGGCTGCCCCTGACGCTGACCTGTCCCAGGAGCCTCATCTCTTCTGACGGGACCTGC
CACTGCACACCAGCCCAGCTCCCGTGTAAATAATTTATTACAAGCATAACATGGAGCTCTTGTTGCACTAAAAAGTGGATTACAAATCTCCTCGACTGCT
TTAGTGGGGAAAGGAATCAATTATTTATGAACTGTCCGGCCCCGAGTCACTCAGCGTTTGCGGGAAAATAAACCACTGGTCCCAGAGCAGAGGAAGGCTA
CTTGAGCCGGACACCAAGCCCGCCTCCAGCACCAAGGGCGGGCAGCACCCTCCGACCCTCCCATGCGGGTGCACACGAAGGGTGAGGCTGACACAGCCAC
TGCGGAGTCCAGGCTGCTAGAGGTGCTCATCCTCACTGCCGTCCTCAGGTGGGTTCGGGCTTCACCGCCTGGCCCTCTGTGGTCACAGAGGGGCTCGGTG
GCCCAGGTGGTGGTTCCGCCTCCAGGGGCAGGGCCTTGTCCTGGGTCTGTGTCAGCGGGTGCACCATGGACATGTGTACATTGAGGTTGTGGGCCTTCTC
AAACCGCCGGCCACACTGGTCACAGGCAAAGTCCAGCTCAGTCTCAGCCTTGTGTTTGGTCATGTGGTACTTGAGGGATGCCCGCTGCCTGCACTGGAAC
CCACAGACCTCACACCTGGGGGACAGAGGCAGATAAGAAGGTGCGAGGGCCACAGCCCTGGGAGGGGGTCCTGACTCACACTTACTGCAAAGGCTTGGCT
CCCGAATGTCGCATTTGGTGGACGAGAAGGTGCTTCCGCTGCTTGAAGGTTTGTCCACATTCGTCACAGATATAGTTCCGCACCTCTGAGAGGGGAGAGT
CCAGTGAGTCCAGGCCCCTGATGCTCCAACCTCCCGGGGGGACGACGATGACAATGTGAAACCATCACAGCTGGGAAGACATTTCTGCACATGGTTCACC
ATGCAGTGGGCCCAAGCAAGGGGCCTATGAGGGCCTCGTTTATTAAGATCTTTAAACTGCTTTATACACTGTCACGTGGCTTCATCAGCTGTGTGCATTT
CAGGATGGTTTTTAAAGAAACCTCAGAAAGCTATTTCCTT
  • FANCA.F13 (forward primer)
  • FANCA.R12 (reverse primer)
  • Single exon skipping

Frame 0

SRDTLCSCLSPGLIKKFQFLMFRLFSEARQPLS
EEDVASLSWRPLHLPSADWQRAALSLWTHRTFRE
VLKEEDVHLTYQDWLHLELEIQPEADALSDTER
SRSYDHSENSDLVFGGRTGNEDIISRLQEMVAD
LELQQDLIVPLGHTPSQEHFLFEIFRRRLQALTS
GWSVAASLQRQRELLMYKRILLRLPSSVLCGSS
FQAEQPITARCEQFFHLVNSEMRNFCSHGGALT
QDITAHFFRGLLNACLRSRDPSLMVDFILAKCQT
KCPLILTSALVWWPSLEPVLLCRWRRHCQSPLP
RELQKLQEGRQFASDFLSPEAASPAPNPDWLSA
AALHFAIQQVREENIRKQLKKLDCEREELLVFLF
FFSLMGLLSSHLTSNSTTDLPKAFHVCAAILEC
LEKRKISWLALFQLTESDLRLGRLLLRVAPDQH
TRLLPFAFYSLLSYFHEDAAIREEAFLHVAVDMY
LKLVQLFVAGDTSTVSPPAGRSLELKGQAGQPR
GTDNKSSSFSAAVNTSVPEKELLTRGRAAG*SW
GLRPRGERRPPEQTAGCP*R*PVPGASSLLTGPA
TAHQPSSRVNNLLQA*HGALVALKSGLQISSTA
LVGKGINYL*TVRPRVTQRLRENKPLVPEQRKA
T*AGHQARLQHQGRAAPSDPPMRVHTKGEADTAT
AESRLLEVLILTAVLRWVRASPPGPLWSQRGSV
AQVVVPPPGAGPCPGSVSAGAPWTCVH*GCGPS
QTAGHTGHRQSPAQSQPCVWSCGT*GMPAACTGT
HRPHTWGTEADKKVRGPQPWEGVLTHTYCKGLA
PECRIWWTRRCFRCLKVCPHSSQI*FRTSERGE
SSESRPLMLQPPGGTTMTM*NHHSWEDISAHGSP
CSGPKQGAYEGLVY*DL*TALYTVTWLHQLCAF
QDGF*RNLRKLFP

Frame 1

HEILCAAAYLQALLKSFSSSCSDCSQRPDSLFL
RRT*PAFPGDPCTFLLQTGRELPSLSGHTEPSE
RC*KRKMFT*LTKTGYTWSWKFNLKLMLFQILNA
QGVMTTQKILIWSLVAAQEMRILFPDCRRW*LT
WSCSKTS*CLSATPLPRSTSSLRFSADGSRL*Q
AGGAWLPAFRDRGSC*CTNGSSSACLRLSSAAAA
SRQNSPSLPDASSSSTWSTLR*ETSAPTEVP*H
RTSLPTSSGAS*TPVCGAETPP*WSTSYWPSAR
RNAP*F*PLLWCGGRAWSLCCSAGGGDTARARCP
GNCRSYKKAGSLPAISSPLRLPPQHPTRTGSQL
LHCTLRFNKSGKKTSGSS*RSWTARERSYWFSF
SSSP*WACCRHI*PQIAPQTCQRLSTFVQQSSSV
*RRGRYPGWHSFS*QRVTSGWGGSSSVWPRIST
PGCCLSLFTVFSPTSMKTRPSGKRPSCMLLWTC
T*SWSSSSWLGIQAQFHLQLAGAWSSRVRQGNPV
ELITKARLFLLQLIPRCPKKSFSHVAELLADRG
DCDPEVSAALQSRQQAAPDADLSQEPHLF*RDL
PLHTSPAPV*IIYYKHNMELLLH*KVDYKSPRLL
*WGKESIIYELSGPESLSVCGKINHWSQSRGRL
LEPDTKPASSTKGGQHPPTLPCGCTRRVRLTQP
LRSPGC*RCSSSLPSSGGFGLHRLALCGHRGARW
PRWWFRLQGQGLVLGLCQRVHHGHVYIEVVGLL
KPPATLVTGKVQLSLSLVFGHVVLEGCPLPALE
PTDLTPGGQRQIRRCEGHSPGRGS*LTLTAKAWL
PNVAFGGREGASAA*RFVHIRHRYSSAPLRGES
PVSPGP*CSNLPGGRR*QCETITAGKTFLHMVH
HAVGPSKGPMRASFIKIFKLLYTLSRGFISCVHF
RMVFKETSESYFL

Frame 2

TRYFVQLLISRPY*KVSVPHVQIVLRGPTASF*
GGRSQPFLETLAPSFCRLAESCPLSLDTQNLPR
GVERGRCSLNLPRLVTPGAGNST*S*CSFRY*T
LKEL*PLRKF*FGLWWPHRK*GYYFQIAGDGS*P
GAAARPHSASRPHPFPGALPL*DFPQTAPGSDK
RVERGCQPSETEGAANVQTDPPPPAFVCPLRQQ
LPGRTAHHCQMRAVLPLGQL*DEKLLLPRRCPDT
GHHCPLLQGPPERLSAEQRPLPDGRLHTGQVPD
EMPLNFDLCSGVVAEPGACAALPVEETLPEPAA
PGTAEATRRPAVCQRFPLP*GCLPSTQPGLALSC
CTALCDSTSQGRKHQEAAKEAGLRERGAIGFPF
LLLLDGPAVVTSDLK*HHRPAKGFPRLCSNPRV
FREEEDILAGTLSVDRE*PQAGAAPPPCGPGSAH
QAAAFRFLQSSLLLP*RRGHQGRGLPACCCGHV
LEAGPALRGWGYKHSFTSSWQEPGAQGSGRATP
WN**QKLVFFCCS*YLGARKRASHTWQSCWLIVG
TATQR*APPSRADSRLPLTLTCPRSLISSDGTC
HCTPAQLPCK*FITSITWSSCCTKKWITNLLDC
FSGERNQLFMNCPAPSHSAFAGK*TTGPRAEEGY
LSRTPSPPPAPRAGSTLRPSHAGAHEG*G*HSH
CGVQAARGAHPHCRPQVGSGFTAWPSVVTEGLG
GPGGGSASRGRALSWVCVSGCTMDMCTLRLWAFS
NRRPHWSQAKSSSVSALCLVMWYLRDARCLHWN
PQTSHLGDRGR*EGARATALGGGPDSHLLQRLG
SRMSHLVDEKVLPLLEGLSTFVTDIVPHL*EGRV
Q*VQAPDAPTSRGDDDDNVKPSQLGRHFCTWFT
MQWAQARGL*GPRLLRSLNCFIHCHVASSAVCI
SGWFLKKPQKAIS

Sequence FANCA.aAug05

CGGGCGCAGGGAGCCGCCGCCGGGGCTGTAGGCGCCAAGGCCATGTCCGACTCGTGGGTCCCGAACTCCGCCTCGGGCCAGGACCCAGGGGGCCGCCGGA
GGGCCTGGGCCGAGCTGCTGGCGGGAAGGGTCAAGAGGGAAAAATATAATCCTGAAAGGGCACAGAAATTAAAGGAATCAGCTGTGCGCCTCCTGCGAAG
CCATCAGGACCTGAATGCCCTTTTGCTTGAGGTAGAAGGTCCACTGTGTAAAAAATTGTCTCTCAGCAAAGTGATTGACTGTGACAGTTCTGAGGCCTAT
GCTAATCATTCTAGTTCATTTATAGGCTCTGCTTTGCAGGATCAAGCCTCAAGGCTGGGGGTTCCCGTGGGTATTCTCTCAGCCGGGATGGTTGCCTCTA
GCGTGGGACAGATCTGCACGGCTCCAGCGGAGACCAGTCACCCTGTGCTGCTGACTGTGGAGCAGAGAAAGAAGCTGTCTTCCCTGTTAGAGTTTGCTCA
GTATTTATTGGCACACAGTATGTTCTCCCGTCTTTCCTTCTGTCAAGAATTATGGAAAATACAGAGTTCTTTGTTGCTTGAAGCGGTGTGGCATCTTCAC
GTACAAGGCATTGTGAGCCTGCAAGAGCTGCTGGAAAGCCATCCCGACATGCATGCTGTGGGATCGTGGCTCTTCAGGAATCTGTGCTGCCTTTGTGAAC
AGATGGAAGCATCCTGCCAGCATGCTGACGTCGCCAGGGCCATGCTTTCTGATTTTGTTCAAATGTTTGTTTTGAGGGGATTTCAGAAAAACTCAGATCT
GAGAAGAACTGTGGAGCCTGAAAAAATGCCGCAGGTCACGGTTGATGTACTGCAGAGAATGCTGATTTTTGCACTTGACGCTTTGGCTGCTGGAGTACAG
GAGGAGTCCTCCACTCACAAGATCGTGAGGTGCTGGTTCGGAGTGTTCAGTGGACACACGCTTGGCAGTGTAATTTCCACAGATCCTCTGAAGAGGTTCT
TCAGTCATACCCTGACTCAGATACTCACTCACAGCCCTGTGCTGAAAGCATCTGATGCTGTTCAGATGCAGAGAGAGTGGAGCTTTGCGCGGACACACCC
TCTGCTCACCTCACTGTACCGCAGGCTCTTTGTGATGCTGAGTGCAGAGGAGTTGGTTGGCCATTTGCAAGAAGTTCTGGAAACGCAGGAGGTTCACTGG
CAGAGAGTGCTCTCCTTTGTGTCTGCCCTGGTTGTCTGCTTTCCAGAAGCGCAGCAGCTGCTTGAAGACTGGGTGGCGCGTTTGATGGCCCAGGCATTCG
AGAGCTGCCAGCTGGACAGCATGGTCACTGCGTTCCTGGTTGTGCGCCAGGCAGCACTGGAGGGCCCCTCTGCGTTCCTGTCATATGCAGACTGGTTCAA
GGCCTCCTTTGGGAGCACACGAGGCTACCATGGCTGCAGCAAGAAGGCCCTGGTCTTCCTGTTTACGTTCTTGTCAGAACTCGTGCCTTTTGAGTCTCCC
CGGTACCTGCAGGTGCACATTCTCCACCCACCCCTGGTTCCCGGCAAGTACCGCTCCCTCCTCACAGACTACATCTCATTGGCCAAGACACGGCTGGCCG
ACCTCAAGGTTTCTATAGAAAACATGGGACTCTACGAGGATTTGTCATCAGCTGGGGACATTACTGAGCCCCACAGCCAAGCTCTTCAGGATGTTGAAAA
GGCCATCATGGTGTTTGAGCATACGGGGAACATCCCAGTCACCGTCATGGAGGCCAGCATATTCAGGAGGCCTTACTACGTGTCCCACTTCCTCCCCGCC
CTGCTCACACCTCGAGTGCTCCCCAAAGTCCCTGACTCCCGTGTGGCGTTTATAGAGTCTCTGAAGAGAGCAGATAAAATCCCCCCATCTCTGTACTCCA
CCTACTGCCAGGCCTGCTCTGCTGCTGAAGAGAAGCCAGAAGATGCAGCCCTGGGAGTGAGGGCAGAACCCAACTCTGCTGAGGAGCCCCTGGGACAGCT
CACAGCTGCACTGGGAGAGCTGAGAGCCTCCATGACAGACCCCAGCCAGCGTGATGTTATATCGGCACAGGTGGCAGTGATTTCTGAAAGACTGAGGGCT
GTCCTGGGCCACAATGAGGATGACAGCAGCGTTGAGATATCAAAGATTCAGCTCAGCATCAACACGCCGAGACTGGAGCCACGGGAACACATGGCTGTGG
ACCTCCTGCTGACGTCTTTCTGTCAGAACCTGATGGCTGCCTCCAGTGTCGCTCCCCCGGAGAGGCAGGGTCCCTGGGCTGCCCTCTTCGTGAGGACCAT
GTGTGGACGTGTGCTCCCTGCAGTGCTCACCCGGCTCTGCCAGCTGCTCCGTCACCAGGGCCCGAGCCTGAGTGCCCCACATGTGCTGGGGTTGGCTGCC
CTGGCCGTGCACCTGGGTGAGTCCAGGTCTGCGCTCCCAGAGGTGGATGTGGGTCCTCCTGCACCTGGTGCTGGCCTTCCTGTCCCTGCGCTCTTTGACA
GCCTCCTGACCTGTAGGACGAGGGATTCCTTGTTCTTCTGCCTGAAATTTTGTACAGCAGCAATTTCTTACTCTCTCTGCAAGTTTTCTTCCCAGTCACG
AGATACTTTGTGCAGCTGCTTATCTCCAGGCCTTATTAAAAAGTTTCAGTTCCTCATGTTCAGATTGTTCTCAGAGGCCCGACAGCCTCTTTCTGAGGAG
GACGTAGCCAGCCTTTCCTGGAGACCCTTGCACCTTCCTTCTGCAGACTGGCAGAGAGCTGCCCTCTCTCTCTGGACACACAGAACCTTCCGAGAGGTGT
TGAAAGAGGAAGATGTTCACTTAACTTACCAAGACTGGTTACACCTGGAGCTGGAAATTCAACCTGAAGCTGATGCTCTTTCAGATACTGAACGGCAGGA
CTTCCACCAGTGGGCGATCCATGAGCACTTTCTCCCTGAGTCCTCGGCTTCAGGGGGCTGTGACGGAGACCTGCAGGCTGCGTGTACCATTCTTGTCAAC
GCACTGATGGATTTCCACCAAAG
CTCAAGGAGTTATGACCACTCAGAAAATTCTGATTTGGTCTTTGGTGGCCGCACAGGAAATGAGGATATTATTTCCA
GATTGCAGGAGATGGTAGCTGACCTGGAGCTGCAGCAAGACCTCATAGTGCCTCTCGGCCACACCCCTTCCCAGGAGCACTTCCTCTTTGAGATTTTCCG
CAGACGGCTCCAGGCTCTGACAAGCGGGTGGAGCGTGGCTGCCAGCCTTCAGAGACAGAGGGAGCTGCTAATGTACAAACGGATCCTCCTCCGCCTGCCT
TCGTCTGTCCTCTGCGGCAGCAGCTTCCAGGCAGAACAGCCCATCACTGCCAGATGCGAGCAGTTCTTCCACTTGGTCAACTCTGAGATGAGAAACTTCT
GCTCCCACGGAGGTGCCCTGACACAGGACATCACTGCCCACTTCTTCAGGGGCCTCCTGAACGCCTGTCTGCGGAGCAGAGACCCCTCCCTGATGGTCGA
CTTCATACTGGCCAAGTGCCAGACGAAATGCCCCTTAATTTTGACCTCTGCTCTGGTGTGGTGGCCGAGCCTGGAGCCTGTGCTGCTCTGCCGGTGGAGG
AGACACTGCCAGAGCCCGCTGCCCCGGGAACTGCAGAAGCTACAAGAAGGCCGGCAGTTTGCCAGCGATTTCCTCTCCCCTGAGGCTGCCTCCCCAGCAC
CCAACCCGGACTGGCTCTCAGCTGCTGCACTGCACTTTGCGATTCAACAAGTCAGGGAAGAAAACATCAGGAAGCAGCTAAAGAAGCTGGACTGCGAGAG
AGAGGAGCTATTGGTTTTCCTTTTCTTCTTCTCCTTGATGGGCCTGCTGTCGTCACATCTGACCTCAAATAGCACCACAGACCTGCCAAAGGCTTTCCAC
GTTTGTGCAGCAATCCTCGAGTGTTTAGAGAAGAGGAAGATATCCTGGCTGGCACTCTTTCAGTTGACAGAGAGTGACCTCAGGCTGGGGCGGCTCCTCC
TCCGTGTGGCCCCGGATCAGCACACCAGGCTGCTGCCTTTCGCTTTTTACAGTCTTCTCTCCTACTTCCATGAAGACGCGGCCATCAGGGAAGAGGCCTT
CCTGCATGTTGCTGTGGACATGTACTTGAAGCTGGTCCAGCTCTTCGTGGCTGGGGATACAAGCACAGTTTCACCTCCAGCTGGCAGGAGCCTGGAGCTC
AAGGGTCAGGGCAACCCCGTGGAACTGATAACAAAAGCTCGTCTTTTTCTGCTGCAGTTAATACCTCGGTGCCCGAAAAAGAGCTTCTCACACGTGGCAG
AGCTGCTGGCTGATCGTGGGGACTGCGACCCAGAGGTGAGCGCCGCCCTCCAGAGCAGACAGCAGGCTGCCCCTGACGCTGACCTGTCCCAGGAGCCTCA
TCTCTTCTGACGGGACCTGCCACTGCACACCAGCCCAGCTCCCGTGTAAATAATTTATTACAAGCATAACATGGAGCTCTTGTTGCACTAAAAAGTGGAT
TACAAATCTCCTCGACTGCTTTAGTGGGGAAAGGAATCAATTATTTATGAACTGTCCGGCCCCGAGTCACTCAGCGTTTGCGGGAAAATAAACCACTGGT
CCCAGAGCAGAGGAAGGCTACTTGAGCCGGACACCAAGCCCGCCTCCAGCACCAAGGGCGGGCAGCACCCTCCGACCCTCCCATGCGGGTGCACACGAAG
GGTGAGGCTGACACAGCCACTGCGGAGTCCAGGCTGCTAGAGGTGCTCATCCTCACTGCCGTCCTCAGGTGGGTTCGGGCTTCACCGCCTGGCCCTCTGT
GGTCACAGAGGGGCTCGGTGGCCCAGGTGGTGGTTCCGCCTCCAGGGGCAGGGCCTTGTCCTGGGTCTGTGTCAGCGGGTGCACCATGGACATGTGTACA
TTGAGGTTGTGGGCCTTCTCAAACCGCCGGCCACACTGGTCACAGGCAAAGTCCAGCTCAGTCTCAGCCTTGTGTTTGGTCATGTGGTACTTGAGGGATG
CCCGCTGCCTGCACTGGAACCCACAGACCTCACACCTGGGGGACAGAGGCAGATAAGAAGGTGCGAGGGCCACAGCCCTGGGAGGGGGTCCTGACTCACA
CTTACTGCAAAGGCTTGGCTCCCGAATGTCGCATTTGGTGGACGAGAAGGTGCTTCCGCTGCTTGAAGGTTTGTCCACATTCGTCACAGATATAGTTCCG
CACCTCTGAGAGGGGAGAGTCCAGTGAGTCCAGGCCCCTGATGCTCCAACCTCCCGGGGGGACGACGATGACAATGTGAAACCATCACAGCTGGGAAGAC
ATTTCTGCACATGGTTCACCATGCAGTGGGCCCAAGCAAGGGGCCTATGAGGGCCTCGTTTATTAAGATCTTTAAACTGCTTTATACACTGTCACGTGGC
TTCATCAGCTGTGTGCATTTCAGGATGGTTTTTAAAGAAACCTCAGAAAGCTATTTCCTT
  • FANCA.F13 (forward primer)
  • FANCA.R12 (reverse primer)
  • Single exon skipping

Frame 0

RAQGAAAGAVGAKAMSDSWVPNSASGQDPGGRR
RAWAELLAGRVKREKYNPERAQKLKESAVRLLRS
HQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAY
ANHSSSFIGSALQDQASRLGVPVGILSAGMVAS
SVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQ
YLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLH
VQGIVSLQELLESHPDMHAVGSWLFRNLCCLCE
QMEASCQHADVARAMLSDFVQMFVLRGFQKNSDL
RRTVEPEKMPQVTVDVLQRMLIFALDALAAGVQ
EESSTHKIVRCWFGVFSGHTLGSVISTDPLKRF
FSHTLTQILTHSPVLKASDAVQMQREWSFARTHP
LLTSLYRRLFVMLSAEELVGHLQEVLETQEVHW
QRVLSFVSALVVCFPEAQQLLEDWVARLMAQAF
ESCQLDSMVTAFLVVRQAALEGPSAFLSYADWFK
ASFGSTRGYHGCSKKALVFLFTFLSELVPFESP
RYLQVHILHPPLVPGKYRSLLTDYISLAKTRLA
DLKVSIENMGLYEDLSSAGDITEPHSQALQDVEK
AIMVFEHTGNIPVTVMEASIFRRPYYVSHFLPA
LLTPRVLPKVPDSRVAFIESLKRADKIPPSLYS
TYCQACSAAEEKPEDAALGVRAEPNSAEEPLGQL
TAALGELRASMTDPSQRDVISAQVAVISERLRA
VLGHNEDDSSVEISKIQLSINTPRLEPREHMAV
DLLLTSFCQNLMAASSVAPPERQGPWAALFVRTM
CGRVLPAVLTRLCQLLRHQGPSLSAPHVLGLAA
LAVHLGESRSALPEVDVGPPAPGAGLPVPALFD
SLLTCRTRDSLFFCLKFCTAAISYSLCKFSSQSR
DTLCSCLSPGLIKKFQFLMFRLFSEARQPLSEE
DVASLSWRPLHLPSADWQRAALSLWTHRTFREV
LKEEDVHLTYQDWLHLELEIQPEADALSDTERQD
FHQWAIHEHFLPESSASGGCDGDLQAACTILVN
ALMDFHQS
SRSYDHSENSDLVFGGRTGNEDIIS
RLQEMVADLELQQDLIVPLGHTPSQEHFLFEIFR
RRLQALTSGWSVAASLQRQRELLMYKRILLRLP
SSVLCGSSFQAEQPITARCEQFFHLVNSEMRNF
CSHGGALTQDITAHFFRGLLNACLRSRDPSLMVD
FILAKCQTKCPLILTSALVWWPSLEPVLLCRWR
RHCQSPLPRELQKLQEGRQFASDFLSPEAASPA
PNPDWLSAAALHFAIQQVREENIRKQLKKLDCER
EELLVFLFFFSLMGLLSSHLTSNSTTDLPKAFH
VCAAILECLEKRKISWLALFQLTESDLRLGRLL
LRVAPDQHTRLLPFAFYSLLSYFHEDAAIREEAF
LHVAVDMYLKLVQLFVAGDTSTVSPPAGRSLEL
KGQGNPVELITKARLFLLQLIPRCPKKSFSHVA
ELLADRGDCDPEVSAALQSRQQAAPDADLSQEPH
LF*RDLPLHTSPAPV*IIYYKHNMELLLH*KVD
YKSPRLL*WGKESIIYELSGPESLSVCGKINHW
SQSRGRLLEPDTKPASSTKGGQHPPTLPCGCTRR
VRLTQPLRSPGC*RCSSSLPSSGGFGLHRLALC
GHRGARWPRWWFRLQGQGLVLGLCQRVHHGHVY
IEVVGLLKPPATLVTGKVQLSLSLVFGHVVLEGC
PLPALEPTDLTPGGQRQIRRCEGHSPGRGS*LT
LTAKAWLPNVAFGGREGASAA*RFVHIRHRYSS
APLRGESPVSPGP*CSNLPGGRR*QCETITAGKT
FLHMVHHAVGPSKGPMRASFIKIFKLLYTLSRG
FISCVHFRMVFKETSESYFL

Frame 1

GRREPPPGL*APRPCPTRGSRTPPRARTQGAAG
GPGPSCWREGSRGKNIILKGHRN*RNQLCASCE
AIRT*MPFCLR*KVHCVKNCLSAK*LTVTVLRPM
LIILVHL*ALLCRIKPQGWGFPWVFSQPGWLPL
AWDRSARLQRRPVTLCC*LWSRERSCLPC*SLL
SIYWHTVCSPVFPSVKNYGKYRVLCCLKRCGIFT
YKAL*ACKSCWKAIPTCMLWDRGSSGICAAFVN
RWKHPASMLTSPGPCFLILFKCLF*GDFRKTQI
*EELWSLKKCRRSRLMYCREC*FLHLTLWLLEYR
RSPPLTRS*GAGSECSVDTRLAV*FPQIL*RGS
SVIP*LRYSLTALC*KHLMLFRCRESGALRGHT
LCSPHCTAGSL*C*VQRSWLAICKKFWKRRRFTG
RECSPLCLPWLSAFQKRSSCLKTGWRV*WPRHS
RAASWTAWSLRSWLCARQHWRAPLRSCHMQTGS
RPPLGAHEATMAAARRPWSSCLRSCQNSCLLSLP
GTCRCTFSTHPWFPASTAPSSQTTSHWPRHGWP
TSRFL*KTWDSTRICHQLGTLLSPTAKLFRMLK
RPSWCLSIRGTSQSPSWRPAYSGGLTTCPTSSPP
CSHLECSPKSLTPVWRL*SL*REQIKSPHLCTP
PTARPALLLKRSQKMQPWE*GQNPTLLRSPWDS
SQLHWES*EPP*QTPASVMLYRHRWQ*FLKD*GL
SWATMRMTAALRYQRFSSASTRRDWSHGNTWLW
TSC*RLSVRT*WLPPVSLPRRGRVPGLPSS*GP
CVDVCSLQCSPGSASCSVTRARA*VPHMCWGWLP
WPCTWVSPGLRSQRWMWVLLHLVLAFLSLRSLT
AS*PVGRGIPCSSA*NFVQQQFLTLSASFLPSH
EILCAAAYLQALLKSFSSSCSDCSQRPDSLFLRR
T*PAFPGDPCTFLLQTGRELPSLSGHTEPSERC
*KRKMFT*LTKTGYTWSWKFNLKLMLFQILNGR
TSTSGRSMSTFSLSPRLQGAVTETCRLRVPFLST
H*WISTK
AQGVMTTQKILIWSLVAAQEMRILFP
DCRRW*LTWSCSKTS*CLSATPLPRSTSSLRFS
ADGSRL*QAGGAWLPAFRDRGSC*CTNGSSSACL
RLSSAAAASRQNSPSLPDASSSSTWSTLR*ETS
APTEVP*HRTSLPTSSGAS*TPVCGAETPP*WS
TSYWPSARRNAP*F*PLLWCGGRAWSLCCSAGGG
DTARARCPGNCRSYKKAGSLPAISSPLRLPPQH
PTRTGSQLLHCTLRFNKSGKKTSGSS*RSWTAR
ERSYWFSFSSSP*WACCRHI*PQIAPQTCQRLST
FVQQSSSV*RRGRYPGWHSFS*QRVTSGWGGSS
SVWPRISTPGCCLSLFTVFSPTSMKTRPSGKRP
SCMLLWTCT*SWSSSSWLGIQAQFHLQLAGAWSS
RVRATPWN**QKLVFFCCS*YLGARKRASHTWQ
SCWLIVGTATQR*APPSRADSRLPLTLTCPRSL
ISSDGTCHCTPAQLPCK*FITSITWSSCCTKKWI
TNLLDCFSGERNQLFMNCPAPSHSAFAGK*TTG
PRAEEGYLSRTPSPPPAPRAGSTLRPSHAGAHE
G*G*HSHCGVQAARGAHPHCRPQVGSGFTAWPSV
VTEGLGGPGGGSASRGRALSWVCVSGCTMDMCT
LRLWAFSNRRPHWSQAKSSSVSALCLVMWYLRD
ARCLHWNPQTSHLGDRGR*EGARATALGGGPDSH
LLQRLGSRMSHLVDEKVLPLLEGLSTFVTDIVP
HL*EGRVQ*VQAPDAPTSRGDDDDNVKPSQLGR
HFCTWFTMQWAQARGL*GPRLLRSLNCFIHCHVA
SSAVCISGWFLKKPQKAIS

Frame 2

GAGSRRRGCRRQGHVRLVGPELRLGPGPRGPPE
GLGRAAGGKGQEGKI*S*KGTEIKGISCAPPAK
PSGPECPFA*GRRSTV*KIVSQQSD*L*QF*GL
C*SF*FIYRLCFAGSSLKAGGSRGYSLSRDGCL*
RGTDLHGSSGDQSPCAADCGAEKEAVFPVRVCS
VFIGTQYVLPSFLLSRIMENTEFFVA*SGVASS
RTRHCEPARAAGKPSRHACCGIVALQESVLPL*T
DGSILPAC*RRQGHAF*FCSNVCFEGISEKLRS
EKNCGA*KNAAGHG*CTAENADFCT*RFGCWST
GGVLHSQDREVLVRSVQWTHAWQCNFHRSSEEVL
QSYPDSDTHSQPCAESI*CCSDAERVELCADTP
SAHLTVPQALCDAECRGVGWPFARSSGNAGGSL
AESALLCVCPGCLLSRSAAAA*RLGGAFDGPGIR
ELPAGQHGHCVPGCAPGSTGGPLCVPVICRLVQ
GLLWEHTRLPWLQQEGPGLPVYVLVRTRAF*VS
PVPAGAHSPPTPGSRQVPLPPHRLHLIGQDTAGR
PQGFYRKHGTLRGFVISWGHY*APQPSSSGC*K
GHHGV*AYGEHPSHRHGGQHIQEALLRVPLPPR
PAHTSSAPQSP*LPCGVYRVSEESR*NPPISVLH
LLPGLLCC*REARRCSPGSEGRTQLC*GAPGTA
HSCTGRAESLHDRPQPA*CYIGTGGSDF*KTEG
CPGPQ*G*QQR*DIKDSAQHQHAETGATGTHGCG
PPADVFLSEPDGCLQCRSPGEAGSLGCPLREDH
VWTCAPCSAHPALPAAPSPGPEPECPTCAGVGC
PGRAPG*VQVCAPRGGCGSSCTWCWPSCPCAL*Q
PPDL*DEGFLVLLPEILYSSNFLLSLQVFFPVT
RYFVQLLISRPY*KVSVPHVQIVLRGPTASF*G
GRSQPFLETLAPSFCRLAESCPLSLDTQNLPRGV
ERGRCSLNLPRLVTPGAGNST*S*CSFRY*TAG
LPPVGDP*ALSP*VLGFRGL*RRPAGCVYHSCQ
RTDGFPPK
LKEL*PLRKF*FGLWWPHRK*GYYFQ
IAGDGS*PGAAARPHSASRPHPFPGALPL*DFP
QTAPGSDKRVERGCQPSETEGAANVQTDPPPPA
FVCPLRQQLPGRTAHHCQMRAVLPLGQL*DEKLL
LPRRCPDTGHHCPLLQGPPERLSAEQRPLPDGR
LHTGQVPDEMPLNFDLCSGVVAEPGACAALPVE
ETLPEPAAPGTAEATRRPAVCQRFPLP*GCLPST
QPGLALSCCTALCDSTSQGRKHQEAAKEAGLRE
RGAIGFPFLLLLDGPAVVTSDLK*HHRPAKGFP
RLCSNPRVFREEEDILAGTLSVDRE*PQAGAAPP
PCGPGSAHQAAAFRFLQSSLLLP*RRGHQGRGL
PACCCGHVLEAGPALRGWGYKHSFTSSWQEPGA
QGSGQPRGTDNKSSSFSAAVNTSVPEKELLTRGR
AAG*SWGLRPRGERRPPEQTAGCP*R*PVPGAS
SLLTGPATAHQPSSRVNNLLQA*HGALVALKSG
LQISSTALVGKGINYL*TVRPRVTQRLRENKPLV
PEQRKAT*AGHQARLQHQGRAAPSDPPMRVHTK
GEADTATAESRLLEVLILTAVLRWVRASPPGPL
WSQRGSVAQVVVPPPGAGPCPGSVSAGAPWTCVH
*GCGPSQTAGHTGHRQSPAQSQPCVWSCGT*GM
PAACTGTHRPHTWGTEADKKVRGPQPWEGVLTH
TYCKGLAPECRIWWTRRCFRCLKVCPHSSQI*FR
TSERGESSESRPLMLQPPGGTTMTM*NHHSWED
ISAHGSPCSGPKQGAYEGLVY*DL*TALYTVTW
LHQLCAFQDGF*RNLRKLFP

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