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Sequences

Sequence ZC3H12D.eAug05

CCTCCCGGGGTTTGTAGCGGAGGAGGAGCGGGCGCCATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAACGGC
CGCGTGGTGAGGCTTGAGCCCCACTCACCTGTCGCCGGGGCCCCCGAGCTTGTCTCCCTACTTTCCCTTCTCTGCGCAGTCCAGGGCAGAGATTGGCTAG
AGCTCGAGCTTCTGGGCTGCTGGTGCCCGCGACTGGGCTATTTTATTAACCGGCCTTGGCTAGAAAAGTTTTTGTGACGGGAATTCGTTTGAGCCTTGGC
AGCCCTTCTCCCGTATTTTGTGTTGGCCAGTCCGGTGGACGGTGCTTGCGGTCATTAATTGCCTTTGGGAGTGTGTGTGTGGGGCGTGTGTGTGTGTGAT
TCGAGCTGCACATACAATACCCATAGCTTTTGTGCCTTTATTGTGTTTACTCGTGAGAGGAACATAGGAACTATTGCTTGGTGTGTAATCGGTTAAATCT
TGATGAAGGCAGTTAGGGTGTGCTTGGCGCAGCGAGTGTGGGCATACCGCTTAGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTA
TTGCCTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGT
GATCAAGCAAGCTTTTTTGAGGCAGAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGCAGTGATCAACATGGAT
CTCAGGAGAAAATCTAGATTATCTTGATGGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAATGAGACCTTTGTT
GACAAGGACTTTG
TACCATATCAGGATATCAGGATAAATCATACGGTGATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGAT
CACCAGAACCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTCAAAGGAAGATCAGCTGAGGAAGTAGAAGAAAT
AAAGGCAGAAAAAGAGGCTAAAACTCAGGCTATACTTTTGGAGATGAATCTCGCTCTGTTATCAGGCTGGAGTGCAATGGCACGATCTTGGCTCACTGCA
ACCTCTGCCTCCCGGGTTCAAGCGATTCTTCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGGCGCCTGCCACCACATCCAGCTAATTTTTTGTAATTG
TAGTAGAGACAGGGTTTCACTGTGTTGCCCAGGCTGGTCTCCAACTCCCGACCTCAGGCGATCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGG
CATGAGCCACCGTGCCCGGCCAATAGAATATTTTTCAATTTAGGTTTATCTACTCTTTCCTCATGATTAGATTCAGGT
  • ZC3H12D.F2 (forward primer)
  • ZC3H12D.R2 (reverse primer)
  • Alternative acceptor

Frame 0

PPGVCSGGGAGAMAVLLETTLGDVVIDLYTEER
PRGEA*APLTCRRGPRACLPTFPSLRSPGQRLAR
ARASGLLVPATGLFY*PALARKVFVTGIRLSLG
SPSPVFCVGQSGGRCLRSLIAFGSVCVGRVCV*
FELHIQYP*LLCLYCVYS*EEHRNYCLVCNRLNL
DEGS*GVLGAASVGIPLSLLEFLETVQNKILQL
LPYSQCTEGFYHTNWRSYRDWPWRRVYLWPTVW
*SSKLF*GRKSPKN*AQEERHSVHGE*WQ*STWI
SGENLDYLDGVHTVFGEVTEGMDIIKKINETFV
DKDF
VPYQDIRINHTVILDDPFDDPPDLLIPDR
SPEPTREQLDSGRIGADEEIDDFKGRSAEEVEEI
KAEKEAKTQAILLEMNLALLSGWSAMARSWLTA
TSASRVQAILLPQAPE*LGLQAPATTSS*FFVI
VVETGFHCVAQAGLQLPTSGDPPALASQSAGITG
MSHRARPIEYFSI*VYLLFPHD*IQ

Frame 1

LPGFVAEEERAPWRFYWRPL*ATSSSTCTPKNG
RVVRLEPHSPVAGAPELVSLLSLLCAVQGRDWL
ELELLGCWCPRLGYFINRPWLEKFL*REFV*ALA
ALLPYFVLASPVDGACGH*LPLGVCVWGVCVCD
SSCTYNTHSFCAFIVFTRERNIGTIAWCVIG*I
LMKAVRVCLAQRVWAYRLACLNFLKLCKIKYYNY
CLIHNVQRDFIIQTGDPTGTGRGGESIFGQLYG
DQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHG
SQEKI*IILMVSIRCLVR*QKAWT*LRKLMRPLL
TRTL
YHIRISG*IIR*F*MIHLMTLLIY*SLID
HQNLQGNN*IVVE*EQMKKLMISKEDQLRK*KK
*RQKKRLKLRLYFWR*ISLCYQAGVQWHDLGSLQ
PLPPGFKRFFCLRLPSSWDYRRLPPHPANFL*L
**RQGFTVLPRLVSNSRPQAILLPWPPKVLGLQ
A*ATVPGQ*NIFQFRFIYSFLMIRFR

Frame 2

SRGL*RRRSGRHGGSTGDHFRRRRHRLVHRRTA
AW*GLSPTHLSPGPPSLSPYFPFSAQSRAEIG*
SSSFWAAGARDWAILLTGLG*KSFCDGNSFEPW
QPFSRILCWPVRWTVLAVINCLWECVCGACVCVI
RAAHTIPIAFVPLLCLLVRGT*ELLLGV*SVKS
**RQLGCAWRSECGHTA*PA*IS*NCAK*NITI
IALFTMYRGILSYKLAILQGLAVEESLSLANCMV
IKQAFLRQKKSQELSTRRKAQCPW*IMAVINMD
LRRKSRLS*WCPYGVW*GDRRHGHN*EN**DLC
*QGLC
TISGYQDKSYGDFR*SI**PS*FINP*SI
TRTYKGTIR*WSNRSR*RN**FQRKIS*GSRRN
KGRKRG*NSGYTFGDESRSVIRLECNGTILAHC
NLCLPGSSDSSASGSRVAGITGACHHIQLIFCNC
SRDRVSLCCPGWSPTPDLRRSSCLGLPKCWDYR
HEPPCPANRIFFNLGLSTLSS*LDSG

Sequence ZC3H12D.aAug05

CTCCTCCCGGGGTTTGTAGCGGAGGAGGAGCGGGCGCCATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAACG
GCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGCCTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGC
GATCCTACAGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCAGAAAAAGTCCCAAGAATTAAGC
ACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGCAGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGATGG
TGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAATGAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATC
AGGATAAATCATACGGTGATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAACCTACAAGGGAACAATTAGATA
GTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTCAAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAGGC
TATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAATGTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAG
GATCTGGAAATAATATTCTCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCCCTCTGTTACGCTTTTATTGAAT
TTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAAATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCAAA
GGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAAAAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAA
GTAAAGCCCAAACAGGATACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACACACAAGTAAAAAACACAAGAAGA
AAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGACTACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTATGA
AGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAACCGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGT
AATAGTCATAAATCAAAATACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCAAAGAAGTCCAAAGATAAAGAAA
AATCTAAGTATAGATGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTGTCTAACTTAAGAGTGCCAGGAAAGCAGATGCTTAGATTTT
GTGTCAAAGCTTGTTATTTTTTTCATACTAGGATTATGGTCTTTAGATTAATACTGATTATATAGAGCACGGAAAGATAAAGAATTGAACATTTTCTTTG
TATACTTTTTTACACTAATTTTATTGTTATACATAAATGGTAGTCTTCATTTTTGAAGTCTTACATTTTCACTCTTTTTTTAATGAAGTATTTCATACTA
CAAAAATACATAAACGTATATATAAAGGGATAATAAATGTAAATATCTGTGTACTCATCAGCCAGCTTAAGATACAGATGTTGTCGACATTTTAGAAGTT
CCCTAAGGCCCTCTCCCTCTCAAATAATTATTTGGAATTTTGTGTTTGTCATTTGTCTATTATAGTTTTACAACATACGTATGTATCTGTAAGTGAAATG
TTAATTTTGTATGTTTCTGAATTTTATATAAATGGCAAAATGTTTACTTCTGTGACTTTCTTTCATTTTTATTGCTATATAGTATTATATAAATATACTA
CAACTTATTCATTCTTGATGGACATTTGAGTTTTGTTTATTTGTTTTTGCTGTTGAAACAATGCTGCTGTGAACATTGTATCTAGCAGTTATATTGCTTG
ATATATTGATAAAATAATGCCAAATTTGTGTTCTGAAGTGACTGTACTAGTTTATACCTCTCTTGGCACTGTGAGTTACCTTTGTTTCATAGCATCTCCA
ACACTTAAGTTCAGACTTAAAAATTTGCCAACCTAGTGGATGTTAAGTGGTATCTACCTGTGATTTCCAGCAAGATTACTGTTATCTCTGCTCTTCCATG
TGTAAGATGTCATTTTTTTCCTGGCTGCTTTTAAGGTTTTCTGTCACTGGTTGTAAGAAGTTTGATTATGATGTG
  • ZC3H12D.F2 (forward primer)
  • ZC3H12D.R2 (reverse primer)
  • Alternative acceptor

Frame 0

LLPGFVAEEERAPWRFYWRPL*ATSSSTCTPKN
GRVPA*IS*NCAK*NITIIALFTMYRGILSYKLA
ILQGLAVEESLSLANCMVIKQAFLRQKKSQELS
TRRKAQCPW*IMAVINMDLSFLSPQEKI*IILM
VSIRCLVR*QKAWT*LRKLMRPLLTRTLYHIRIS
G*IIR*F*MIHLMTLLIY*SLIDHQNLQGNN*I
VVE*EQMKKLMISKEDQLRK*KK*RQKKRLKLR
LYFWRWWETYLMQILNLQKMYCLCVN*TQ*PQMR
IWK*YSLDLGQ*EVVKLSETGRQESPSVTLLLN
LKRKKIVRKHSSKWTMCL*MTEEYMWILASRLQ
RLNGKEKVGNTPRVISRSMKKNRINHLIWF*KIK
*SPNRIQNTILY*MSRPKTQNQVTHTQVKNTRR
KPITVLKRKKMRTTCQSKILIRISIEKWGLVTM
KKKKAVGRNKRVKRETELRTEVVADLERGMAIIV
IVINQNTKQIFMKEKGVKRETEAEVQRSPKIKK
NLSIDER*RGRIERLTYLLLSNLRVPGKQMLRF
CVKACYFFHTRIMVFRLILII*STER*RIEHFLC
ILFYTNFIVIHKW*SSFLKSYIFTLFLMKYFIL
QKYINVYIKG**M*ISVYSSASLRYRCCRHFRS
SLRPSPSQIIIWNFVFVICLL*FYNIRMYL*VKC
*FCMFLNFI*MAKCLLL*LSFIFIAI*YYINIL
QLIHS*WTFEFCLFVFAVETMLL*TLYLAVILL
DILIK*CQICVLK*LY*FIPLLAL*VTFVS*HLQ
HLSSDLKICQPSGC*VVSTCDFQQDYCYLCSSM
CKMSFFSWLLLRFSVTGCKKFDYDV

Frame 1

SSRGL*RRRSGRHGGSTGDHFRRRRHRLVHRRT
AACLLEFLETVQNKILQLLPYSQCTEGFYHTNW
RSYRDWPWRRVYLWPTVW*SSKLF*GRKSPKN*A
QEERHSVHGE*WQ*STWISVSYHHRRKSRLS*W
CPYGVW*GDRRHGHN*EN**DLC*QGLCTISGY
QDKSYGDFR*SI**PS*FINP*SITRTYKGTIR*
WSNRSR*RN**FQRKIS*GSRRNKGRKRG*NSG
YTFGDGGRPT*CRY*TSRKCTVCV*IEPSDHR*
GSGNNIL*IWANKKL*SYPRLEDRRVPLLRFY*I
*KGRRL*ESILQNGQCAYR*QKNTCGF*PVGCK
G*MERKRWEIHQE*FQGV*KRTG*TT*FGSER*
SKAQTGYKIRSYIR*AGRRLKIKSLTHK*KTQEE
NPSLF*RERR*GLHANQKY*SGYL*RNGVWSL*
RRRKLLGETKE*KERPNSEPKS*PISREGWPL*
**S*IKIPNRSL*KRKE*KERPKQKSKEVQR*RK
I*V*MKDEEAELRG*HIYSCLT*ECQESRCLDF
VSKLVIFFILGLWSLD*Y*LYRARKDKELNIFF
VYFFTLILLLYINGSLHF*SLTFSLFF**SISYY
KNT*TYI*RDNKCKYLCTHQPA*DTDVVDILEV
P*GPLPLK*LFGILCLSFVYYSFTTYVCICK*N
VNFVCF*ILYKWQNVYFCDFLSFLLLYSII*IYY
NLFILDGHLSFVYLFLLLKQCCCEHCI*QLYCL
IY**NNAKFVF*SDCTSLYLSWHCELPLFHSIS
NT*VQT*KFANLVDVKWYLPVISSKITVISALPC
VRCHFFPGCF*GFLSLVVRSLIMM

Frame 2

PPGVCSGGGAGAMAVLLETTLGDVVIDLYTEER
PRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTG
DPTGTGRGGESIFGQLYGDQASFFEAEKVPRIK
HKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDG
VHTVFGEVTEGMDIIKKINETFVDKDFVPYQDI
RINHTVILDDPFDDPPDLLIPDRSPEPTREQLD
SGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQA
ILLEMVGDLPDADIKPPENVLFVCKLNPVTTDE
DLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIE
FEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAK
VKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDK
VKPKQDTKYDLILDEQAEDSKSSHSHTSKKHKK
KTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHYE
EEESCWEKQKSEKRDRTQNRSRSRSRERDGHYS
NSHKSKYQTDLYERERSKKRDRSRSPKKSKDKE
KSKYR*KMKRQN*EANIFTLV*LKSARKADA*IL
CQSLLFFSY*DYGL*INTDYIEHGKIKN*TFSL
YTFLH*FYCYT*MVVFIFEVLHFHSFFNEVFHT
TKIHKRIYKGIINVNICVLISQLKIQMLSTF*KF
PKALSLSNNYLEFCVCHLSIIVLQHTYVSVSEM
LILYVSEFYINGKMFTSVTFFHFYCYIVLYKYT
TTYSFLMDI*VLFICFCC*NNAAVNIVSSSYIA*
YIDKIMPNLCSEVTVLVYTSLGTVSYLCFIASP
TLKFRLKNLPT*WMLSGIYL*FPARLLLSLLFH
V*DVIFFLAAFKVFCHWL*EV*L*C

Sequence ZC3H12D.bAug05

CTCCTCCCGGGGTTTGTAGCGGAGGAGGAGCGGGCGCCATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAACG
GCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGCCTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGC
GATCCTACAGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCAGAAAAAGTCCCAAGAATTAAGC
ACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGCAGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGATGG
TGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAATGAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATC
AGGATAAATCATACGGTGATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAACCTACAAGGGAACAATTAGATA
GTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTCAAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAGGC
TATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAATGTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAG
GATCTGGAAATAATATTCTCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCCCTCTGTTACGCTTTTATTGAAT
TTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAAATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCAAA
GGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAAAAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAA
GTAAAGCCCAAACAGGAGTATCCTTACCAAGAATCAGTTAGTTAATATGTATTGACCATCTGCTATGTGTAGAGTACTGTGTGATGTGTTATGAAAAGTC
ACAAAAGCTATAGAAGACAAATTTCCTGAATCCAGATTGTTTGTGAGGAAGTAACTTAGTTTATCATTATCTCTTTTCAAGATCTTACATATACTTTGCT
TTTTCACTATACTCTAGTGTATGGAGAAAGTTAAGGTTGAAAGAAATCTGTAGCCTAATTCTTTTATTAATACTTTCCTACTCTAGGTTAAGTACTTTCC
TACTTATCATATTCTCAGAAGATCTTCAGGTAAGATTTGTATTTTCTTTTTGGGGGGGCTTTCCTGCAAAAACCGGAAAGCCTACTAGACAAATTCTAAA
AGAGCTGTAACACTAGCTTTGTATTTTCTATATGTAATTGTAATGTTTTCTATTCCTCATAATCTGAAATTTATTATTGCTAAGCTGATTCCTTTGTGCT
ACCTGCTGTTATGTTTAACTTCCTTACCTAGATAACAAAATCCTATTGTTCTCTGTTACAATGTTAGGTTACTTCTTTCGGTATAGTATATTGTAATTTC
AACAACAAATATTTATTGGGCTTCTACTGAGTGCAAGAGTTCACTTTTGTAGGATTAAAAAGAAATCCATTGACTTGACCATTTACTTCAGGTTCTTCCA
AGTTTTAGGTTGAGACATGTAAAAAGTAATGAAACATACTGTTTATATTATGACATTTAAATTGGTTCACTAATTTTTCCCATTATAAATATATCACTCC
ACACATTGCAAGAGTTATTTCTGTTGTGATTCTTTCTGGTAAATGGTGTCTTTGTTTCCTAGTTTCTATTGGTATTTCAGGGATTTTTTTTTCTACATAT
GTAGACATTTCTTTTCCAAGTACTCATCAAGAAAGATGAATATCAGGTTGACCTGGTCTATATTCATGTTTTAAGATGTGTTGATTGTCTGCAATGTATA
AAGCATTGTGAGGTGTTAGAACTGGATAAAGTCTGAAGAAAGCTGATAAAATCAGCATAAGGAGAGGCTTTCACATGCAGACAGACCAAATAAGAAGCTG
AGATTTGGTCAGAAAAGATCAACATTCAAGGGCATATGATTGAAAGCTCAAAATCATAAAAGTTATGAATGGAATACATTTGATATTTATTTACCAAATC
TTTGAAATTAAATTTAGGCTTATTACTAATAACTATAGCTGGAAGAAGGGGAATTTTAAGTTTTCTTTAATTTTAATTAATTGAAACAACCACACATATA
CTTATATGGAATAGTGTAGCTTCTAGCCTTTTGAAGTTGATGCAAAAGAGAGAAATGCTGTTAGGGGATAGTCTGATGTGCAGATAAATTGTCTTAAGTC
AGTTAAGAAGGCATGGTGGCATCTACTTTATGGGCCTTAAGAGAAAGAGAAATTAGTGAAAGTATAGATTATTAATAAGAAGAATCAAAAATAGAAGTAG
GAGAAGGAAATAACTTGGCCCAACTAGACCTCAGGGAAGAAATTGGAGAAAAGATGGCAGTGAGCTCACAAATTTGTGTTTTATTCAAAGGGAGAGGGAT
TTGATGTCTTCTGAAAGTGTTGATTTTAGTGATAAGTACAAGATAGTGGGTAAGTGAGGAAGCAGGAGATACAACAAGTGTCTTGAGGGTATGACAAGTA
ATCAACAGATACTTAGTAAGCATCTGTCACATACTCAGTTTAGCAATGAAAGATATTCAGTTCCTGCCCACATAGAGCTTACATTTTGCTGTTGGAGTGG
GGAAAAAAAACCAGACATTAAATAATTACCATTCTAAATACGTCTAAATTATTGCAAATAAATATTGCAAAGAGATATGGGACTCATAATAGGGGGCGAG
AGGTTAGAGAATACCCTCAACACTGAAAAAAGTGGCATTAAAGCTGTGACACTAATATGGGAGATAGGAGGGCTGAGGTGGAGTGCTGTCTTCTTTCGTA
TGATCCATCATTTAAGAAATAGGCTGGGTGCAGTGGCTCATGGGAGTGCTGTCTTCTTTCGTATGATCCATCATTTAAGAAATAGGCTGGGTGCAGTGGC
TCATGCCTGTAATCCCAGCAGTGTGGGAGGCCGAGGTGGGAGGATTGCTGGAGCCCAGAAGTTTGAGAGCAGCCAGGGCAATATAGCGAGACCCGGTCTC
CATTTTTTTTTTCTTA
  • ZC3H12D.F2 (forward primer)
  • ZC3H12D.R2 (reverse primer)
  • Alternative acceptor

Frame 0

LLPGFVAEEERAPWRFYWRPL*ATSSSTCTPKN
GRVPA*IS*NCAK*NITIIALFTMYRGILSYKLA
ILQGLAVEESLSLANCMVIKQAFLRQKKSQELS
TRRKAQCPW*IMAVINMDLSFLSPQEKI*IILM
VSIRCLVR*QKAWT*LRKLMRPLLTRTLYHIRIS
G*IIR*F*MIHLMTLLIY*SLIDHQNLQGNN*I
VVE*EQMKKLMISKEDQLRK*KK*RQKKRLKLR
LYFWRWWETYLMQILNLQKMYCLCVN*TQ*PQMR
IWK*YSLDLGQ*EVVKLSETGRQESPSVTLLLN
LKRKKIVRKHSSKWTMCL*MTEEYMWILASRLQ
RLNGKEKVGNTPRVISRSMKKNRINHLIWF*KIK
*SPNRSILTKNQLVNMY*PSAMCRVLCDVL*KV
TKAIEDKFPESRLFVRK*LSLSLSLFKILHILC
FFTIL*CMEKVKVERNL*PNSFINTFLL*VKYFP
TYHILRRSSGKICIFFLGGLSCKNRKAY*TNSK
RAVTLALYFLYVIVMFSIPHNLKFIIAKLIPLC
YLLLCLTSLPR*QNPIVLCYNVRLLLSV*YIVIS
TTNIYWASTECKSSLL*D*KEIH*LDHLLQVLP
SFRLRHVKSNETYCLYYDI*IGSLIFPIINISL
HTLQELFLL*FFLVNGVFVS*FLLVFQGFFFLHM
*TFLFQVLIKKDEYQVDLVYIHVLRCVDCLQCI
KHCEVLELDKV*RKLIKSA*GEAFTCRQTK*EA
EIWSEKINIQGHMIESSKS*KL*MEYI*YLFTKS
LKLNLGLLLITIAGRRGILSFL*F*LIETTTHI
LIWNSVASSLLKLMQKREMLLGDSLMCR*IVLS
QLRRHGGIYFMGLKRKRN**KYRLLIRRIKNRSR
RRK*LGPTRPQGRNWRKDGSELTNLCFIQRERD
LMSSESVDFSDKYKIVGK*GSRRYNKCLEGMTS
NQQILSKHLSHTQFSNERYSVPAHIELTFCCWSG
EKKPDIK*LPF*IRLNYCK*ILQRDMGLIIGGE
RLENTLNTEKSGIKAVTLIWEIGGLRWSAVFFR
MIHHLRNRLGAVAHGSAVFFRMIHHLRNRLGAVA
HACNPSSVGGRGGRIAGAQKFESSQGNIARPGL
HFFFL

Frame 1

SSRGL*RRRSGRHGGSTGDHFRRRRHRLVHRRT
AACLLEFLETVQNKILQLLPYSQCTEGFYHTNW
RSYRDWPWRRVYLWPTVW*SSKLF*GRKSPKN*A
QEERHSVHGE*WQ*STWISVSYHHRRKSRLS*W
CPYGVW*GDRRHGHN*EN**DLC*QGLCTISGY
QDKSYGDFR*SI**PS*FINP*SITRTYKGTIR*
WSNRSR*RN**FQRKIS*GSRRNKGRKRG*NSG
YTFGDGGRPT*CRY*TSRKCTVCV*IEPSDHR*
GSGNNIL*IWANKKL*SYPRLEDRRVPLLRFY*I
*KGRRL*ESILQNGQCAYR*QKNTCGF*PVGCK
G*MERKRWEIHQE*FQGV*KRTG*TT*FGSER*
SKAQTGVSLPRIS*LICIDHLLCVEYCVMCYEKS
QKL*KTNFLNPDCL*GSNLVYHYLFSRSYIYFA
FSLYSSVWRKLRLKEICSLILLLILSYSRLSTF
LLIIFSEDLQVRFVFSFWGGFPAKTGKPTRQILK
EL*H*LCIFYM*L*CFLFLII*NLLLLS*FLCA
TCCYV*LPYLDNKILLFSVTMLGYFFRYSIL*F
QQQIFIGLLLSARVHFCRIKKKSIDLTIYFRFFQ
VLG*DM*KVMKHTVYIMTFKLVH*FFPL*IYHS
THCKSYFCCDSFW*MVSLFPSFYWYFRDFFFYI
CRHFFSKYSSRKMNIRLTWSIFMF*DVLIVCNV*
SIVRC*NWIKSEES**NQHKERLSHADRPNKKL
RFGQKRSTFKGI*LKAQNHKSYEWNTFDIYLPN
L*N*I*AYY**L*LEEGEF*VFFNFN*LKQPHIY
LYGIV*LLAF*S*CKREKCC*GIV*CADKLS*V
S*EGMVASTLWALREREISESIDY**EESKIEV
GEGNNLAQLDLREEIGEKMAVSSQICVLFKGRGI
*CLLKVLILVISTR*WVSEEAGDTTSVLRV*QV
INRYLVSICHILSLAMKDIQFLPT*SLHFAVGV
GKKNQTLNNYHSKYV*IIANKYCKEIWDS**GAR
G*RIPSTLKKVALKL*H*YGR*EG*GGVLSSFV
*SII*EIGWVQWLMGVLSSFV*SII*EIGWVQW
LMPVIPAVWEAEVGGLLEPRSLRAARAI*RDPVS
IFFFL

Frame 2

PPGVCSGGGAGAMAVLLETTLGDVVIDLYTEER
PRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTG
DPTGTGRGGESIFGQLYGDQASFFEAEKVPRIK
HKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDG
VHTVFGEVTEGMDIIKKINETFVDKDFVPYQDI
RINHTVILDDPFDDPPDLLIPDRSPEPTREQLD
SGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQA
ILLEMVGDLPDADIKPPENVLFVCKLNPVTTDE
DLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIE
FEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAK
VKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDK
VKPKQEYPYQESVS*YVLTICYV*STV*CVMKS
HKSYRRQIS*IQIVCEEVT*FIIISFQDLTYTLL
FHYTLVYGES*G*KKSVA*FFY*YFPTLG*VLS
YLSYSQKIFR*DLYFLFGGAFLQKPESLLDKF*
KSCNTSFVFSICNCNVFYSS*SEIYYC*ADSFVL
PAVMFNFLT*ITKSYCSLLQC*VTSFGIVYCNF
NNKYLLGFY*VQEFTFVGLKRNPLT*PFTSGSS
KF*VETCKK**NILFIL*HLNWFTNFSHYKYITP
HIARVISVVILSGKWCLCFLVSIGISGIFFSTY
VDISFPSTHQER*ISG*PGLYSCFKMC*LSAMY
KAL*GVRTG*SLKKADKISIRRGFHMQTDQIRS*
DLVRKDQHSRAYD*KLKIIKVMNGIHLIFIYQI
FEIKFRLITNNYSWKKGNFKFSLILIN*NNHTY
TYME*CSF*PFEVDAKERNAVRG*SDVQINCLKS
VKKAWWHLLYGP*EKEKLVKV*IINKKNQK*K*
EKEITWPN*TSGKKLEKRWQ*AHKFVFYSKGEG
FDVF*KC*F***VQDSG*VRKQEIQQVS*GYDK*
STDT**ASVTYSV*Q*KIFSSCPHRAYILLLEW
GKKTRH*IITILNTSKLLQINIAKRYGTHNRGR
EVREYPQH*KKWH*SCDTNMGDRRAEVECCLLSY
DPSFKK*AGCSGSWECCLLSYDPSFKK*AGCSG
SCL*SQQCGRPRWEDCWSPEV*EQPGQYSETRS
PFFFS

Sequence ZC3H12D.cAug05

CTCCTCCCGGGGTTTGTAGCGGAGGAGGAGCGGGCGCCATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAACG
GCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGCCTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGC
GATCCTACAGGGACTGGCCGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCAGAAAAAGTCCCAAGAATTAAGC
ACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGCAGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGATGG
TGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAATGAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATC
AGCCTCAAACTCCTGGATTCAAGTGATCCCCCTGCCTCAGCCTCCTGAGTAACTGGGACTGTAAACATGACAGCCGCACTTCACAGTTACACTGGGGCTG
GATGAGTCAGGACTTTTGCTCTCAAATCTACAGGTGGGTTAGGAGAAAACTGACATGAGCTCAGTCAGAGTTTATTGCCACCTTCCTGGTGCCGTTTCTT
CTAGCCAGCTACTGCTCTGGGTTTGAGGAGAAAGGGCCTACTCATTTGCAATGGCTAAGAAGTGAGATGGTGCTGCCAGAGTGCCGACCCCCGAATCCTC
AGGGGCCCTGGCCTCTCCTGGCAGCATCCACCCATTCTGGTTACCATGCCAGCCCTCCAGACTATGGCTCATTTGTTCACTAGAGGGCTTGGTAGCTTGG
CAGTTTGGGGAAAGCGGGAGGCACTTCCCAGGGGAAGACCAGAGAAGACCAGCCCCCAGCCCCACAAACTTCCTGCAGCCTGCTGGTGCAGAGGGAACTC
TTGCCAGTTTACTACCAGATTTCATGAGATGTTGGCAGGCCTGGGGGGCACCTCAGGGAGAGTGCGTTCCCAGCCCTTTCATTCATGGTAAATGACACCC
CTGGGGCATACAGTGATGTTAGGTTTTTCTCTGAAACTTCTTTGATCTTTTAAAACCAGATTCTTAGCATGGATTGAGCCAGATCTGTTCCTTACCCCCT
TTTTCCAAAGAGGACGGTGCACCCACAATTTTACCATAACATATTGGGATCTCAGGCCTGAAGATGACCCATTCTTTGTAAATAAGTTGGATTTTCCTCA
TAATATGTGTGGGTTTTTTTGGTGGGGGAGGAGGGGGCAGGGTTCCGCTCTGTCATCTGTCACCCAGGCTGGAGTGCAGTAGTGCAATCAGGGCTCACTG
CAGCTTTGACTTCCCAGGCTCAAGCAATCCTTGTGCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACCGCGCCTGGCCCCGGGCCTTTATT
TTTATTTTTAACCTTTTGACAAGACACGCTTTCTATTAGAGCTGCTTTTTGTCTCCCTGGTTCGATTTCCTGATGTAGAATCATTGTGTTGCTGATTTTT
CAGTTGAAAATGTTTAAACTGCTTCCTTTGAGAACAAGTTTGAGTTTTAGTAAGCTGTAAAGCTGTTTTATTTGATTCACTGTGAATAAGACAAGTACAA
AAAAAAAAGTGAAAACACAGCACATTTCAAGTGTAATCCCCTTTGGAATTTTTCCAAAGTCTTAGTATATGTCTTTGTGACAGAAAAACATAGATGGGAC
AGGTAAATGAAATGTTCCTAGTTGCATAGCTGTTAACCATGCTTCAAAAATAAATCATTTTTTACCACACCTG
  • ZC3H12D.F2 (forward primer)
  • ZC3H12D.R2 (reverse primer)
  • Alternative acceptor

Frame 0

LLPGFVAEEERAPWRFYWRPL*ATSSSTCTPKN
GRVPA*IS*NCAK*NITIIALFTMYRGILSYKLA
ILQGLAVEESLSLANCMVIKQAFLRQKKSQELS
TRRKAQCPW*IMAVINMDLSFLSPQEKI*IILM
VSIRCLVR*QKAWT*LRKLMRPLLTRTLYHIRIS
ASNSWIQVIPLPQPPE*LGL*T*QPHFTVTLGL
DESGLLLSNLQVG*EKTDMSSVRVYCHLPGAVS
SSQLLLWV*GERAYSFAMAKK*DGAARVPTPESS
GALASPGSIHPFWLPCQPSRLWLICSLEGLVAW
QFGESGRHFPGEDQRRPAPSPTNFLQPAGAEGT
LASLLPDFMRCWQAWGAPQGECVPSPFIHGK*HP
WGIQ*C*VFL*NFFDLLKPDS*HGLSQICSLPP
FSKEDGAPTILP*HIGISGLKMTHSL*ISWIFL
IICVGFFGGGGGGRVPLCHLSPRLECSSAIRAHC
SFDFPGSSNPCASASQSAGIIGVSHRAWPRAFI
FIFNLLTRHAFY*SCFLSPWFDFLM*NHCVADF
SVENV*TASFENKFEF**AVKLFYLIHCE*DKYK
KKSENTAHFKCNPLWNFSKVLVYVFVTEKHRWD
R*MKCS*LHSC*PCFKNKSFFTTP

Frame 1

SSRGL*RRRSGRHGGSTGDHFRRRRHRLVHRRT
AACLLEFLETVQNKILQLLPYSQCTEGFYHTNW
RSYRDWPWRRVYLWPTVW*SSKLF*GRKSPKN*A
QEERHSVHGE*WQ*STWISVSYHHRRKSRLS*W
CPYGVW*GDRRHGHN*EN**DLC*QGLCTISGY
QPQTPGFK*SPCLSLLSNWDCKHDSRTSQLHWGW
MSQDFCSQIYRWVRRKLT*AQSEFIATFLVPFL
LASYCSGFEEKGPTHLQWLRSEMVLPECRPPNP
QGPWPLLAASTHSGYHASPPDYGSFVH*RAW*LG
SLGKAGGTSQGKTREDQPPAPQTSCSLLVQREL
LPVYYQIS*DVGRPGGHLRESAFPALSFMVNDT
PGAYSDVRFFSETSLIF*NQILSMD*ARSVPYPL
FPKRTVHPQFYHNILGSQA*R*PILCK*VGFSS
*YVWVFLVGEEGAGFRSVICHPGWSAVVQSGLT
AALTSQAQAILVPQPPKVLGL*A*ATAPGPGPLF
LFLTF*QDTLSIRAAFCLPGSIS*CRIIVLLIF
QLKMFKLLPLRTSLSFSKL*SCFI*FTVNKTST
KKKVKTQHISSVIPFGIFPKS*YMSL*QKNIDGT
GK*NVPSCIAVNHASKINHFLPHL

Frame 2

PPGVCSGGGAGAMAVLLETTLGDVVIDLYTEER
PRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTG
DPTGTGRGGESIFGQLYGDQASFFEAEKVPRIK
HKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDG
VHTVFGEVTEGMDIIKKINETFVDKDFVPYQDI
SLKLLDSSDPPASAS*VTGTVNMTAALHSYTGA
G*VRTFALKSTGGLGEN*HELSQSLLPPSWCRFF
*PATALGLRRKGLLICNG*EVRWCCQSADPRIL
RGPGLSWQHPPILVTMPALQTMAHLFTRGLGSL
AVWGKREALPRGRPEKTSPQPHKLPAACWCRGNS
CQFTTRFHEMLAGLGGTSGRVRSQPFHSW*MTP
LGHTVMLGFSLKLL*SFKTRFLAWIEPDLFLTP
FFQRGRCTHNFTITYWDLRPEDDPFFVNKLDFPH
NMCGFFWWGRRGQGSALSSVTQAGVQ*CNQGSL
QL*LPRLKQSLCLSLPKCWDYRREPPRLAPGLY
FYF*PFDKTRFLLELLFVSLVRFPDVESLCC*FF
S*KCLNCFL*EQV*VLVSCKAVLFDSL*IRQVQ
KKK*KHSTFQV*SPLEFFQSLSICLCDRKT*MG
QVNEMFLVA*LLTMLQK*IIFYHT

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