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Sequences

Sequence VDR.dAug05

VDR.F3 (forward primer)
VDR.R3 (reverse primer)
Single exon skipping

Frame 0

LLVKRRQRSRVRRERGTACPPAGRTRAPEPSPA GRRNGL*PPLIASCQAPCTLLCLPPSILIASLWG MEAMAASTSLPDPGDFDRNVPRICGVCGDRATG FHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDC RITKDNRRHCQACRLKRCVDIGMMKEFILTDEEV QRKREMILKRKEEEALKDSLRPKLSEEQQRIIA ILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSH PSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSS SFSNLDLSEEDSDDPSVTLELSQLSMLPHLADL VSYSIQKVIGFAKMIPG

Frame 1

CLSKGGSGAVCAGSAEQLVHPPAGPGLLNLAQL DGEMDSSLL**PHARPRAHCFACLPQSS*LLFG GWRQWRPALPCLTLETLTGTCPGSVGCVETEPLA FTSML*PVKAAKASSGEA*SGRHYSPAPSTGTA ASPRTTDATARPAGSNAVWTSA**RSSF*QMRK CRGSGR*S*SGRRRRP*RTVCGPSCLRSSSASLP YCWTPTIRPTTPPTPTSASSGLQFV*MMVEGAI LPGPTPDTLPASLGTPPPPAQITVSPLQT*WTR PASPIWI*VKKIQMTLL*P*SCPSSPCCPTWLTW SVTASKRSLALLR*YQD

Frame 2

ACQKAAAEPCAPGARNSLSTRRPDQGS*T*PSW TEKWTLASSDSLMPGPVHIALLASLNPHSFSLG DGGNGGQHFPA*PWRL*PERAPDLWGVWRPSHW LSLQCYDL*RLQRLLQAKHEAEGTIHLPLQRGLP HHQGQPTPLPGLPAQTLCGHRHDEGVHSDR*GS AEEAGDDPEAEGGGGLEGQSAAQAV*GAAAHHC HTAGRPP*DLRPHLLRLLPVPASSSCE*WWREPS FQAQLQTHSQLLWGLLLLLLRSLYHLFRHDGLV QLLQSGSE*RRFR*PFCDPRAVPALHAAPPG*P GQLQHPKGHWLC*DDTR

Sequence VDR.bAug05

CTGCTTGTCAAAAGGCGGCAGCGGAGCCGTGTGCGCCGGGAGCGCGGAACAGCTTGTCCACCCGCCGGCCGGACCAGGGCTCCTGAACCTAGCCCAGCTG GACGGAGAAATGGACTCTAGCCTCCTCTGATAGCCTCATGCCAGGCCCCGTGCACATTGCTTTGCTTGCCTCCCTCAATCCTCATAGCTTCTCTTTGGGA AGCCTTTGGGTCTGAAGTGTCTGTGAGACCTCACAGAAGAGCACCCCTGGGCTCCACTTACCTGCCCCCTGCTCCTTCAGGGATGGAGGCAATGGCGGCC AGCACTTCCCTGCCTGACCCTGGAGACTTTGACCGGAACGTGCCCCGGATCTGTGGGGTGTGTGGAGACCGAGCCACTGGCTTTCACTTCAATGCTATGA CCTGTGAAGGCTGCAAAGGCTTCTTCAGGCGAAGCATGAAGCGGAAGGCACTATTCACCTGCCCCTTCAACGGGGACTGCCGCATCACCAAGGACAACCG ACGCCACTGCCAGGCCTGCCGGCTCAAACGCTGTGTGGACATCGGCATGATGAAGGAGTTCATTCTGACAGATGAGGAAGTGCAGAGGAAGCGGGAGATG ATCCTGAAGCGGAAGGAGGAGGAGGCCTTGAAGGACAGTCTGCGGCCCAAGCTGTCTGAGGAGCAGCAGCGCATCATTGCCATACTGCTGGACGCCCACC ATAAGACCTACGACCCCACCTACTCCGACTTCTGCCAGTTCCGGCCTCCAGTTCGTGTGAATGATGGTGGAGGGAGCCATCCTTCCAGGCCCAACTCCAG ACACACTCCCAGCTTCTCTGGGGACTCCTCCTCCTCCTGCTCAGATCACTGTATCACCTCTTCAGACATGATGGACTCGTCCAGCTTCTCCAATCTGGAT CTGAGTGAAGAAGATTCAGATGACCCTTCTGTGACCCTAGAGCTGTCCCAGCTCTCCATGCTGCCCCACCTGGCTGACCTGGTCAGTTACAGCATCCAAA AGGTCATTGGCTTTGCTAAGATGATACCAGGATTCAGAGACCTCACCTCTGAGGACCAGATCGTACTGCTGAAGTCAAGTGCCATTGAGGTCATCATGTT GCGCTCCAATGAGTCCTTCACCATGGACGACATGTCCTGGACCTGTGGCAACCAAGACTACAAGTACCGCGTCAGTGACGTGACCAAAGCCGGACACAGC CTGGAGCTGATTGAGCCCCTCATCAAGTTCCAGGTGGGACTGAAGAAGCTGAACTTGCATGAGGAGGAGCATGTCCTGCTCATGGCCATCTGCATCGTCT CCCCAGATCGTCCTGGGGTGCAGGACGCCGCGCTGATTGAGGCCATCCAGGACCGCCTGTCCAACACACTGCAGACGTACATCCGCTGCCGCCACCCGCC CCCGGGCAGCCACCTGCTCTATGCCAAGATGATCCAGAAGCTAGCCGACCTGCGCAGCCTCAATGAGGAGCACTCCAAGCAGTACCGCTGCCTCTCCTTC CAGCCTGAGTGCAGCATGAAGCTAACGCCCCTTGTGCTCGAAGTGTTTGGCAATGAGATCTCCTGACTAGGACAGCCTGTGGCGGTGCCTGGGTGGGGCT GCTCCTCCAGGGCCACGTGCCAGGCCCGGGGCTGGCGGCTACTCAGCAGCCCTCCTCACCCCGTCTGGGGTTCAGCCCCTCCTCTGCCACCTCCCCTATC CACCCAGCCCATTCTCTCTCCTGTCCAACCTAACCCCTTTCCTGCGGGCTTTTCCCCGGTCCCTTGAGACCTCAGCCATGAGGAGTTGCTGTTTGTTTGA CAAAGAAACCCAAGTGGGGGCAGAGGGCAGAGGCTGGAGGCAGGGCCTTGCCCAGAGATGCCTCCACCGCTGCCTAAGTGGCTGCTGACTGATGTTGAGG GAACAGACAGGAGAAATGCATCCATTCCTCAGGGACAGAGACACCTGCACCTCCCCCCACTGCAGGCCCCGCTTGTCCAGCGCCTAGTGGGGTCTCCCTC TCCTGCCTACTCACGATAAATAATCGGCCCACAGCTCCCACCCCACCCCCTTCAGTGCCCACCAACATCCCATTGCCCTGGTTATATTCTCACGGGCAGT AGCTGTGGTGAGGTGGGTTTTCTTCCCATCACTGGAGCACCAGGCACGAACCCACCTGCTGAGAGACCCAAGGAGGAAAAACAGACAAAAACAGCCTCAC AGAAGAATATGACAGCTGTCCCTGTCACCAAGCTCACAGTTCCTCGCCCTGGGTCTAAGGGGTTGGTTGAGGTGGAAGCCCTCCTTCCACGGATCCATGT AGCAGGACTGAATTGTCCCCAGTTTGCAGAAAAGCACCTGCCGACCTCGTCCTCCCCCTGCCAGTGCCTTACCTCCTGCCCAGGAGAGCCAGCCCTCCCT GTCCTCCTCGGATCACCGAGAGTAGCCGAGAGCCTGCTCCCCCACCCCCTCCCCAGGGGAGAGGGTCTGGAGAAGCAGTGAGCCGCATCTTCTCCATCTG GCAGGGTGGGATGGAGGAGAAGAATTTTCAGACCCCAGCGGCTGAGTCATGATCTCCCTGCCGCCTCAATGTGGTTGCAAGGCCGCTGTTCACCCACAGG GCTAAGAGCTAGCGCTGCCGCACCCCAGAGTGTGGGAAGGGAGAGCGGGGCAGTCTCGGGTGGCTAGTCAGAGAGAGTGTTTGGGGGTTCCGTGATGTAG GGTAAGGTGCCTTCTTATTCTCACTCCACCACCCAAAAGTCAAAAGGTGCCTGTGAGGCAGGGGCGGAGTGATACAACTTCAAGTGCATGCTCTCTGCAG CCAGCCCAGCCCAGCTGGTGGGAAGCGTCTGTCCGTTTACTCCAAGGTGGGGTCTTTGTGAGAGTGAGCTGTAGGTGTGCGGGACCGGTACAGAAAGGCG TTCTTCGAGGTGGATCACAGAGGCTTCTTCAGATCAGTGCTTGAGTTTGGGGAATGCGGCCGCATTCCCTGAGTCACCAGGAATGTTAAAGTCAGTGGGA ACGTGACTGCCCCAACTCCTGGAAGCTGTGTCCTTGCACCTGCATCCGTAGTTCCCTGAAAACCCAGAGAGGAATCAGACTTCACACTGCAAGAGCCTTG GTGTCCACCTGGCCCCATGTCTCTCAGAATTCTTCAGGTGGAAAAACATCTGAAAGCCACGTTCCTTACTGCAGAATAGCATATATATCGCTTAATCTTA AATTTATTAGATATGAGTTGTTTTCAGACTCAGACTCCATTTGTATTATAGTCTAATATACAGGGTAGCAGGTACCACTGATTTGGAGATATTTATGGGG GGAGAACTTACATTGTGAAACTTCTGTACATTAATTATTATTGCTGTTGTTATTTTACAAGGGTCTAGGGAGAGACCCTTGTTTGATTTTAGCTGCAGAA CGTATTGGTCCAGCTTGCTCTTCAGTGGGAGAAAACACTTGTAAGTTGCTAAACGAGTCAATCCCCTCATTCAGGAAAACTGACAGAGGAGGGCGTGACT CACCCAAGCATATATAACTAGCTAGAAGTGGGCCAGGACAGGCCCGGCGCGGTGGCTCACGCCTGTAATCCCAGCAGTTTGGGAGGTCGAGGTAGGTGGA TCACCTGAGGTCGGGAGTTCGAGACCAACCTGACCAACATGGAGAAACCCTGTCTCTATTAAAAATACAAAAAAAAAAAAAAAAAAAATAGCCGGGCATG GTGGCGCAAGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGAAGAATTGAACCCAGGAGGTGGAGGTTGCAGTGAGCTGAGATCGTGCCGTTACTCT CCAACCTGGACAACAAGAGCGAAACTCCGTCTTAGAAGTGGACCAGGACAGGACCAGATTTTGGAGTCATGGTCCGGTGTCCTTTTCACTACACCATGTT TGAGCTCAGACCCCCACTCTCATTCCCCAGGTGGCTGACCCAGTCCCTGGGGGAAGCCCTGGATTTCAGAAAGAGCAAGTCTGGATCTGGGACCCTTTCC TTCCTTCCCTGGCTTGTAACTCCACCAACCCATCAGAAGGAGAAGGAAGGAGACTCACCTCTGCCTCAATGTGAATCAGACCCTACCCCACCACGATGTG GCCCTGGCCTGCTGGGCTCTCCACCTCAGCCTTGGATAATGCTGTTGCCTCATCTATAACATGCATTTGTCTTTGTAATGTCACCACCTTCCCAGCTCTC CCTCTGGCCCTGCCTTCTTCGGGGAACTCCTGGAAATATCAGTTACTCAGCCCTGGGCCCCACCACCTAGGCCACTCCTCCAAAGGAAGTCTAGGAGCTG GGAGGAAAAGAAAAGAGGGGAAAATGAGTTTTTATGGGGCTGAACGGGGAGAAAAGGTCATCATCGATTCTACTTTAGAATGAGAGTGTGAAATAGACAT TTGTAAATGTAAAACTTTTAAGGTATATCATTATAACTGAAGGAGAAGGTGCCCCAAAATGCAAGATTTTCCACAAGATTCCCAGAGACAGGAAAATCCT CTGGCTGGCTAACTGGAAGCATGTAGGAGAATCCAAGCGAGGTCAACAGAGAAGGCAGGAATGTGTGGCAGATTTAGTGAAAGCTAGAGATATGGCAGCG AAAGGATGTAAACAGTGCCTGCTGAATGATTTCCAAAGAGAAAAAAAGTTTGCCAGAAGTTTGTCAAGTCAACCAATGTAGAAAGCTTTGCTTATGGTAA TAAAAATGGCTCATACTTATATAGCACTTACTTTGTTGCAAGTACTGCTGTAAATAAATGCTTTATGCAAACCAA

VDR.F3 (forward primer)
VDR.R3 (reverse primer)
Single exon skipping

Frame 0

LLVKRRQRSRVRRERGTACPPAGRTRAPEPSPA GRRNGL*PPLIASCQAPCTLLCLPPSILIASLWE AFGSEVSVRPHRRAPLGSTYLPPAPSGMEAMAA STSLPDPGDFDRNVPRICGVCGDRATGFHFNAM TCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNR RHCQACRLKRCVDIGMMKEFILTDEEVQRKREM ILKRKEEEALKDSLRPKLSEEQQRIIAILLDAH HKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSR HTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLD LSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQ KVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIML RSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHS LELIEPLIKFQVGLKKLNLHEEEHVLLMAICIV SPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPP PGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSF QPECSMKLTPLVLEVFGNEIS*LGQPVAVPGWG CSSRATCQARGWRLLSSPPHPVWGSAPPLPPPLS TQPILSPVQPNPFPAGFSPVP*DLSHEELLFV* QRNPSGGRGQRLEAGPCPEMPPPLPKWLLTDVE GTDRRNASIPQGQRHLHLPPLQAPLVQRLVGSPS PAYSR*IIGPQLPPHPLQCPPTSHCPGYILTGS SCGEVGFLPITGAPGTNPPAERPKEEKQTKTAS QKNMTAVPVTKLTVPRPGSKGLVEVEALLPRIHV AGLNCPQFAEKHLPTSSSPCQCLTSCPGEPALP VLLGSPRVAESLLPHPLPRGEGLEKQ*AASSPS GRVGWRRRIFRPQRLSHDLPAASMWLQGRCSPTG LRASAAAPQSVGRESGAVSGG*SERVFGGSVM* GKVPSYSHSTTQKSKGACEAGAE*YNFKCMLSA ASPAQLVGSVCPFTPRWGLCESEL*VCGTGTERR SSRWITEASSDQCLSLGNAAAFPESPGMLKSVG T*LPQLLEAVSLHLHP*FPENPERNQTSHCKSL GVHLAPCLSEFFRWKNI*KPRSLLQNSIYIA*S* IY*I*VVFRLRLHLYYSLIYRVAGTTDLEIFMG GELTL*NFCTLIIIAVVILQGSRERPLFDFSCR TYWSSLLFSGRKHL*VAKRVNPLIQEN*QRRA*L TQAYITS*KWARTGPARWLTPVIPAVWEVEVGG SPEVGSSRPT*PTWRNPVSIKNTKKKKKKIAGH GGASL*SQLLRRLRQKN*TQEVEVAVS*DRAVTL QPGQQERNSVLEVDQDRTRFWSHGPVSFSLHHV *AQTPTLIPQVADPVPGGSPGFQKEQVWIWDPF LPSLACNSTNPSEGEGRRLTSASM*IRPYPTTMW PWPAGLSTSALDNAVASSITCICLCNVTTFPAL PLALPSSGNSWKYQLLSPGPHHLGHSSKGSLGA GRKRKEGKMSFYGAERGEKVIIDSTLE*ECEIDI CKCKTFKVYHYN*RRRCPKMQDFPQDSQRQENP LAG*LEACRRIQARSTEKAGMCGRFSES*RYGS ERM*TVPAE*FPKRKKVCQKFVKSTNVESFAYGN KNGSYLYSTYFVASTAVNKCFMQT

Frame 1

CLSKGGSGAVCAGSAEQLVHPPAGPGLLNLAQL DGEMDSSLL**PHARPRAHCFACLPQSS*LLFG KPLGLKCL*DLTEEHPWAPLTCPLLLQGWRQWRP ALPCLTLETLTGTCPGSVGCVETEPLAFTSML* PVKAAKASSGEA*SGRHYSPAPSTGTAASPRTT DATARPAGSNAVWTSA**RSSF*QMRKCRGSGR* S*SGRRRRP*RTVCGPSCLRSSSASLPYCWTPT IRPTTPPTPTSASSGLQFV*MMVEGAILPGPTP DTLPASLGTPPPPAQITVSPLQT*WTRPASPIWI *VKKIQMTLL*P*SCPSSPCCPTWLTWSVTASK RSLALLR*YQDSETSPLRTRSYC*SQVPLRSSC CAPMSPSPWTTCPGPVATKTTSTASVT*PKPDTA WS*LSPSSSSRWD*RS*TCMRRSMSCSWPSASS PQIVLGCRTPR*LRPSRTACPTHCRRTSAAATR PRAATCSMPR*SRS*PTCAASMRSTPSSTAASPS SLSAA*S*RPLCSKCLAMRSPD*DSLWRCLGGA APPGPRARPGAGGYSAALLTPSGVQPLLCHLPY PPSPFSLLSNLTPFLRAFPRSLETSAMRSCCLFD KETQVGAEGRGWRQGLAQRCLHRCLSGC*LMLR EQTGEMHPFLRDRDTCTSPHCRPRLSSA*WGLP LLPTHDK*SAHSSHPTPFSAHQHPIALVIFSRAV AVVRWVFFPSLEHQARTHLLRDPRRKNRQKQPH RRI*QLSLSPSSQFLALGLRGWLRWKPSFHGSM *QD*IVPSLQKSTCRPRPPPASALPPAQESQPSL SSSDHRE*PRACSPTPSPGERVWRSSEPHLLHL AGWDGGEEFSDPSG*VMISLPPQCGCKAAVHPQ G*ELALPHPRVWEGRAGQSRVASQRECLGVP*CR VRCLLILTPPPKSQKVPVRQGRSDTTSSACSLQ PAQPSWWEASVRLLQGGVFVRVSCRCAGPVQKG VLRGGSQRLLQISA*VWGMRPHSLSHQEC*SQWE RDCPNSWKLCPCTCIRSSLKTQRGIRLHTARAL VSTWPHVSQNSSGGKTSESHVPYCRIAYISLNL KFIRYELFSDSDSICIIV*YTG*QVPLIWRYLWG ENLHCETSVH*LLLLLLFYKGLGRDPCLILAAE RIGPACSSVGENTCKLLNESIPSFRKTDRGGRD SPKHI*LARSGPGQARRGGSRL*SQQFGRSR*VD HLRSGVRDQPDQHGETLSLLKIQKKKKKK*PGM VAQACNPSYSGG*GRRIEPRRWRLQ*AEIVPLL SNLDNKSETPS*KWTRTGPDFGVMVRCPFHYTMF ELRPPLSFPRWLTQSLGEALDFRKSKSGSGTLS FLPWLVTPPTHQKEKEGDSPLPQCESDPTPPRC GPGLLGSPPQPWIMLLPHL*HAFVFVMSPPSQLS LWPCLLRGTPGNISYSALGPTT*ATPPKEV*EL GGKEKRGK*VFMGLNGEKRSSSILL*NESVK*T FVNVKLLRYIIITEGEGAPKCKIFHKIPRDRKIL WLANWKHVGESKRGQQRRQECVADLVKARDMAA KGCKQCLLNDFQREKKFARSLSSQPM*KALLMV IKMAHTYIALTLLQVLL*INALCKP

Frame 2

ACQKAAAEPCAPGARNSLSTRRPDQGS*T*PSW TEKWTLASSDSLMPGPVHIALLASLNPHSFSLG SLWV*SVCETSQKSTPGLHLPAPCSFRDGGNGG QHFPA*PWRL*PERAPDLWGVWRPSHWLSLQCYD L*RLQRLLQAKHEAEGTIHLPLQRGLPHHQGQP TPLPGLPAQTLCGHRHDEGVHSDR*GSAEEAGD DPEAEGGGGLEGQSAAQAV*GAAAHHCHTAGRPP *DLRPHLLRLLPVPASSSCE*WWREPSFQAQLQ THSQLLWGLLLLLLRSLYHLFRHDGLVQLLQSG SE*RRFR*PFCDPRAVPALHAAPPG*PGQLQHPK GHWLC*DDTRIQRPHL*GPDRTAEVKCH*GHHV ALQ*VLHHGRHVLDLWQPRLQVPRQ*RDQSRTQ PGAD*APHQVPGGTEEAELA*GGACPAHGHLHRL PRSSWGAGRRAD*GHPGPPVQHTADVHPLPPPA PGQPPALCQDDPEASRPAQPQ*GALQAVPLPLL PA*VQHEANAPCARSVWQ*DLLTRTACGGAWVGL LLQGHVPGPGLAATQQPSSPRLGFSPSSATSPI HPAHSLSCPT*PLSCGLFPGPLRPQP*GVAVCL TKKPKWGQRAEAGGRALPRDASTAA*VAAD*C*G NRQEKCIHSSGTETPAPPPTAGPACPAPSGVSL SCLLTINNRPTAPTPPPSVPTNIPLPWLYSHGQ *LW*GGFSSHHWSTRHEPTC*ETQGGKTDKNSLT EEYDSCPCHQAHSSSPWV*GVG*GGSPPSTDPC SRTELSPVCRKAPADLVLPLPVPYLLPRRASPP CPPRITESSREPAPPPPPQGRGSGEAVSRIFSIW QGGMEEKNFQTPAAES*SPCRLNVVARPLFTHR AKS*RCRTPECGKGERGSLGWLVRESVWGFRDV G*GAFLFSLHHPKVKRCL*GRGGVIQLQVHALCS QPSPAGGKRLSVYSKVGSL*E*AVGVRDRYRKA FFEVDHRGFFRSVLEFGECGRIP*VTRNVKVSG NVTAPTPGSCVLAPASVVP*KPREESDFTLQEPW CPPGPMSLRILQVEKHLKATFLTAE*HIYRLIL NLLDMSCFQTQTPFVL*SNIQGSRYH*FGDIYG GRTYIVKLLYINYYCCCYFTRV*GETLV*F*LQN VLVQLALQWEKTLVSC*TSQSPHSGKLTEEGVT HPSIYN*LEVGQDRPGAVAHACNPSSLGGRGRW IT*GREFETNLTNMEKPCLY*KYKKKKKKNSRAW WRKPVIPATQEAEAEELNPGGGGCSELRSCRYS PTWTTRAKLRLRSGPGQDQILESWSGVLFTTPC LSSDPHSHSPGG*PSPWGKPWISERASLDLGPFP SFPGL*LHQPIRRRRKETHLCLNVNQTLPHHDV ALACWALHLSLG*CCCLIYNMHLSL*CHHLPSS PSGPAFFGELLEISVTQPWAPPPRPLLQRKSRSW EEKKRGENEFLWG*TGRKGHHRFYFRMRV*NRH L*M*NF*GISL*LKEKVPQNARFSTRFPETGKS SGWLTGSM*ENPSEVNREGRNVWQI**KLEIWQR KDVNSAC*MISKEKKSLPEVCQVNQCRKLCLW* *KWLILI*HLLCCKYCCK*MLYANQ